CA2401654A1 - Identification et utilisation de molecules effectrices et allosteriques pour alterer l'expression genique - Google Patents

Identification et utilisation de molecules effectrices et allosteriques pour alterer l'expression genique Download PDF

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Publication number
CA2401654A1
CA2401654A1 CA002401654A CA2401654A CA2401654A1 CA 2401654 A1 CA2401654 A1 CA 2401654A1 CA 002401654 A CA002401654 A CA 002401654A CA 2401654 A CA2401654 A CA 2401654A CA 2401654 A1 CA2401654 A1 CA 2401654A1
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CA
Canada
Prior art keywords
effector
control module
allosteric control
rna
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002401654A
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English (en)
Inventor
William S. Marshall
Anastasia Khvorova
Sumedha Jayasena
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/US2001/006615 external-priority patent/WO2001064956A2/fr
Publication of CA2401654A1 publication Critical patent/CA2401654A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La présente invention concerne la mise au points d'un module de régulation allostérique dans lequel l'ARN catalytique forme une partie/est lié à un domaine d'ARN de liaison d'effecteur ou à un aptamère. Ces produits de synthèse placent l'activité de l'ARN catalytique sous le contrôle de l'effecteur et exige la présence d'un effecteur approprié pour l'activation ou l'inactivation. En outre, cette invention concerne un dispositif d'identification de molécules effectrices utiles, ainsi que leur utilisation pour élaborer des aptamères correspondants. Cette invention implique à la fois l'élaboration de séquence d'ARN qui se lient à l'effecteur, et un procédé de sélection dans lequel les modules de régulation allostérique sont identifiés par leur fonction catalytique en présence et en l'absence de l'effecteur. Les ARN catalytiques régulables obtenus peuvent être utilisés pour modifier l'expression d'une molécule d'ARN cible de manière contrôlée.
CA002401654A 2000-03-01 2001-03-01 Identification et utilisation de molecules effectrices et allosteriques pour alterer l'expression genique Abandoned CA2401654A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US18624800P 2000-03-01 2000-03-01
US60/186,248 2000-03-01
US72925800A 2000-11-28 2000-11-28
US09/729,258 2000-11-28
PCT/US2001/006615 WO2001064956A2 (fr) 2000-03-01 2001-03-01 Identification et utilisation de molecules effectrices et allosteriques pour alterer l'expression genique

Publications (1)

Publication Number Publication Date
CA2401654A1 true CA2401654A1 (fr) 2001-09-07

Family

ID=26881904

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002401654A Abandoned CA2401654A1 (fr) 2000-03-01 2001-03-01 Identification et utilisation de molecules effectrices et allosteriques pour alterer l'expression genique

Country Status (1)

Country Link
CA (1) CA2401654A1 (fr)

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued