CA2367396A1 - Methode de selection de variantes de recombinase a specificite modifiee - Google Patents

Methode de selection de variantes de recombinase a specificite modifiee Download PDF

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Publication number
CA2367396A1
CA2367396A1 CA002367396A CA2367396A CA2367396A1 CA 2367396 A1 CA2367396 A1 CA 2367396A1 CA 002367396 A CA002367396 A CA 002367396A CA 2367396 A CA2367396 A CA 2367396A CA 2367396 A1 CA2367396 A1 CA 2367396A1
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CA
Canada
Prior art keywords
recombination
sites
variant
site
recombination sites
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002367396A
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English (en)
Inventor
Brian Lee Sauer
Andreas Walter Rufer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oklahoma Medical Research Foundation
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Individual
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Filing date
Publication date
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Publication of CA2367396A1 publication Critical patent/CA2367396A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des variantes de recombinase Cre augmentant la spécificité du site de recombinaison. Plus particulièrement, l'invention concerne des variantes servant de médiation dans la recombinaison entre des séquences autres que la séquence loxP et autres séquences du site lox sur lesquelles la recombinase Cre de type sauvage est active. En général, les variantes Cre de l'invention servent de médiation dans la recombinaison efficace entre les sites lox sur lesquels peut agir la Cre de type sauvage (désignés sous le nom de sites lox de type sauvage), entre des sites lox variants n'étant pas utilisés de manière efficace par la Cre de type sauvage (désignés sous le nom de sites lox variants), et entre un site lox sauvage et un site lox variant. L'invention concerne également des méthodes de recombinaison d'acides nucléiques à l'aide des variantes Cre de l'invention. Par exemple, ces variantes Cre peuvent être utilisées dans toute méthode ou technique dans lesquelles peut être utilisée la recombinase Cre (ou d'autres recombinases semblables telles que la FLP). De plus, les variantes Cre permettent d'effectuer des recombinaisons diverses. En effet, les variantes Cre permettent une recombinaison beaucoup plus efficace entre les sites lox de type sauvage et les sites lox variants. La commande d'une recombinaison de ce type permet de réaliser des recombinaisons séquentielles plus sophistiquées et d'obtenir des résultats impossibles à atteindre avec la recombinase Cre de type sauvage.
CA002367396A 1999-04-06 2000-04-06 Methode de selection de variantes de recombinase a specificite modifiee Abandoned CA2367396A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12797799P 1999-04-06 1999-04-06
US60/127,977 1999-04-06
PCT/US2000/009154 WO2000060091A2 (fr) 1999-04-06 2000-04-06 Methode de selection de variantes de recombinase a specificite modifiee

Publications (1)

Publication Number Publication Date
CA2367396A1 true CA2367396A1 (fr) 2000-10-12

Family

ID=22432967

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002367396A Abandoned CA2367396A1 (fr) 1999-04-06 2000-04-06 Methode de selection de variantes de recombinase a specificite modifiee

Country Status (4)

Country Link
EP (1) EP1169458A2 (fr)
AU (1) AU4205500A (fr)
CA (1) CA2367396A1 (fr)
WO (1) WO2000060091A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888732A (en) 1995-06-07 1999-03-30 Life Technologies, Inc. Recombinational cloning using engineered recombination sites
DE69836092T2 (de) 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
ATE443074T1 (de) 1999-03-02 2009-10-15 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
WO2001005961A1 (fr) * 1999-07-14 2001-01-25 Clontech Laboratories, Inc. Procedes faisant intervenir la recombinase pour la production de vecteurs d'expression et compositions permettant d'atteindre ce resultat
US6746870B1 (en) 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
ES2355493T3 (es) * 2000-02-18 2011-03-28 The Board Of Trustees Of The Leland Stanford Junior University Recombinasas alteradas para la modificación del genoma.
WO2002008409A2 (fr) 2000-07-21 2002-01-31 The United States Of America, As Represented By The Secretary Of Agriculture Procedes de remplacement, transposition et empilage de l'adn dans des genomes eucaryotes
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
GB0029375D0 (en) * 2000-12-01 2001-01-17 Total Fina Elf Substrate linked directed evolution (slide)
US6977165B2 (en) 2001-01-18 2005-12-20 Clontech Laboratories, Inc. Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same
CA2441937A1 (fr) 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Plate-formes basees sur des chromosomes
WO2005054438A2 (fr) 2003-12-01 2005-06-16 Invitrogen Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
EP2067858A1 (fr) 2007-12-07 2009-06-10 Universidad de Sevilla Modèles d'animaux pour maladies neurodégénératives
ES2337973B8 (es) 2008-05-16 2011-07-21 Proyecto De Biomedicina Cima, S.L. Adenovirus auxiliares auto-inactivantes para la produccion de adenovirus recombinantes de alta capacidad.
ES2523016B1 (es) 2013-05-20 2015-09-09 3P Biopharmaceuticals Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes

Also Published As

Publication number Publication date
WO2000060091A2 (fr) 2000-10-12
WO2000060091A3 (fr) 2001-05-10
EP1169458A2 (fr) 2002-01-09
AU4205500A (en) 2000-10-23

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EEER Examination request
FZDE Discontinued