CA2346868C - Sustained release pharmaceutical composition and method of releasing pharmaceutically active agent - Google Patents

Abstract

The present invention is directed to solid, sustained-release, oral dosage form pharmaceutical compositions which contain therapeutic amounts of a pharmaceutically active agent, hydroxypropyl methyl cellulose and anon-ionic, hydrophilic polymer selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 90,000 to 1,300,000, hydroxypropyl cellulose having a number average molecular weight of 370,000 to 1,500,000, and poly(ethylene oxide) having a number average molecular weight ranging from 100,000 to 500,000.

Description

WO 00/21525 PCr/EP99/07627 SUSTAINED RELEASE PHARMACEUTICAL COMPOSITION AND METHOD
OF RELEASING PHARMACEUTICALLY ACTIVE AGENT
Field of the Invention The present invention relates to sustained-release, oral dosage forms of pharmaceutical compositions.

Background of the Invention Conventional sustained-release, oral dosage forms of phannaceutical compositions are utilized for a number of reasons. Such compositions provide for delivery of a pharmaceutically active agent over an extended time period, versus nonsustained-release, or immediate release, compositions, in which all of the pharmaceutically active agent is delivered over a short period of time immediately after the composition is ingested. Because this immediate release results in the active agent's peak concentration in the patient's system followed by concentrations reduced below therapeutically effective levels, nonsustained release compositions are typically administered in several, separate dosages throughout the day. Conventional sustained-release compositions therefore provide advantages over nonsustained-release compositions by providing the ability to reduce the number of doses required in a given period of time, e.g. single dosing versus multiple dosing, improving patient compliance, and providing a more constant active agent concentration in the blood over extended periods of time.
Althougli sustained release compositions may typically allow a single administration of the active agent's required dosage over a desired delivery period, for instance, a single daily dosage, such compositions may nonetheless exhibit premature release of significant amounts of the active agent. For a number of reasons, such a premature release, or "burst," of the pharmaceutically active agent can decrease the overall therapeutic efficiency of the active agent being delivered. One such problem occurs when the organ to which the active agent is delivered processes the active agent at a constant rate. Consequently, the premature release results in an ainount of active agent in excess of the amount the organ is capable of processing in a given time, i.e. the organ is "flooded" with active agent. Much of the active agent may therefore pass by the organ witliout being processed and essentially is lost in the user's system where it provides no therapeutic affect.

U.S. Patent 5,376,383 teaches in Example 8 a matrix delivery system containing the therapeutic agent lovastatin, a hydroxypropyl cellulose (KLUCEL LF), and a hydroxypropylmethyl cellulose (METHOCEL E5 and METHOCEL K15M). KLUCEL LF, according to the manufacturer's literature, has a molecular weight range of about 95,000. At such a low molecular weight, the KLUCEL LF is not known to have any effect on the matrix delivery system's release profile. The '383 patent remains silent regarding the release profile soon after administration.
It is desirable to develop a composition that provides all of the advantages of conventional sustained-release compositions, yet minimizes the premature release of significant amounts of active agent.

Brief Description of the Figures Figure 1 is a graph depicting dissolution versus time of a composition in water, wherein the amount of hydroxypropylmetliyl cellulose in the composition has been varied.
Figure 2 is a graph depicting dissolution versus time of compositions in acetate buffer, pH 4Ø
Figure 3 is a graph depicting dissolution versus time of compositions in phosphate buffer, pH 6.8.
Figure 4 is a graph depicting dissolution versus time of compositions in water.
Figure 5 is a graph depicting dissolution versus time of a composition containing both liydroxypropylmethyl cellulose and hydroxypropyl cellulose, wherein the dissolution medium is phosphate buffer, pH 6.8.
Figure 6 is a graph depicting dissolution versus time of a composition containing hydroxypropylmethyl cellulose but not hydroxypropyl cellulose, wherein the dissolution medium is phosphate buffer, pH 6.8.

Summary of the Invention The present invention is directed to a pharmaceutical composition containing a pharmaceutically active agent, hydroxypropyl methyl cellulose, and a non-ionic, hydrophilic polymer selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 90,000 to 1,300,000, liydroxypropyl cellulose having a number average molecular weight of 370,000 to 1,500,000, and poly(ethylene oxide) having a number average molecular weight ranging froni 100,000 to 500,000.

The present invention is also directed to a method of releasing a pharmaceutically active agent in a mammal, wherein the method includes orally administering the pharmaceutically active agent to the mammal as part of a pharmaceutical composition including the pharmaceutically active agent, hydroxypropyl methyl cellulose; and a non-ionic, hydrophilic polymer selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 90,000 to 1,300,000, hydroxypropyl cellulose having a number average molecular weight of 370,000 to 1,500,000, and poly(ethylene oxide) having a number average molecular weight ranging from 370,000 to 500,000.

According to one aspect of the present invention, there is provided a pharmaceutical composition, comprising:
fluvastatin or a pharmaceutically acceptable salt thereof, hydroxypropyl methyl cellulose; and a non-ionic, hydrophilic polymer selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 90,000 to 1,300,000, hydroxypropyl cellulose having a number average molecular weight of 370,000 to 1,500,000 and poly(ethylene oxide) having a number average molecular weight ranging from 100,000 to 500,000.

According to another aspect of the present invention, there is provided use of hydroxypropyl methyl cellulose and a non-ionic, hydrophilic polymer in preparation of a pharmaceutical composition comprising fluvastatin or a pharmaceutically acceptable salt thereof, wherein the pharmaceutical composition is for sustained release of the fluvastatin or the pharmaceutically acceptable salt thereof and wherein the non-ionic, hydrophilic polymer is selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 90,000 to 1,300,000, hydroxypropyl cellulose having a number average molecular weight of 370,000 to 1,500,000 and poly(ethylene oxide) having a number average molecular weight ranging from 100,000 to 500,000.

Detailed Description of the Invention It surprisingly has been discovered that compositions that contain, in addition to hydroxypropylmethyl cellulose (hereinafter "HPMC"), at least one non-ionic hydrophilic polymer, prevent premature release of the pharmaceutically active agent from the composition.
As used herein, "premature release" means that a substantial amount of the pharmaceutically active agent is released in a short period of time after ingestion of the composition, for instance in a burst, such that the amount of active agent converted to a bioavailable form is in excess of the amount of the active agent that can be processed efficiently at the targeted active site. Prematurely released active agent may therefore bypass the targeted active site without being processed. As a result, therapeutic efficacy of the pharmaceutical composition may be reduced.

Non-ionic, hydrophilic polymers used in the pharmaceutical composition are selected from the group consisting of hydroxy ethylcellulose (hereinafter "HEC") having a number average molecular weight ranging from 90,000 to 1,300,000, preferably about 1,000,000 to about 1,300,000, hydroxypropylcellulose (hereinafter "HPC") having a number average molecular weight of 370,000 to 1,500,000, preferably 850,000 to 1,500,000, more preferably 1,000,000 to 1,200,000, and poly(ethylene oxide) (hereinafter "PEO") having a number average molecular weight 3a ranging from 100,000 to 500,000, preferably 150,000 to 300,000, more preferably 200,000.
Examples of HEC polymers are commercially available from Hercules Incorporated, Aqualon Division, under the tradename NATROSOLO 250H or NATROSOLO 250L.
Examples of HPC polymers are also available from Hercules Incorporation, Aqualon Division under the tradename KLUCELO
or KLUCEL HXF, and examples of PEO polymers are available from Union Carbide Corporation under the tradename POLYOXO.
Methods of making the non-ionic, 3b hydrophilic polymers suitable for use in the compositions described herein are known by those skilled in the art.
The non-ionic, hydrophilic polymer may be present in the pharmaceutical composition in an amount ranging from about 1 to about 20 weight percent, preferably about 3 to about 12 weight percent, more preferably about 4 to about 7 weight percent. The non-ionic, hydrophilic polymer is present in an amount sufficient to prevent premature release of the pharmaceutically active agent.
As mentioned, the pharmaceutical compositions described herein also contain HPMC in an amount effective to provide sustained-release of the pharmaceutically active agent upon ingestion. As used herein, "sustained-release" means that the pharmaceutically active agent is released from the dosage form over an extended period of time, for example greater than about six hours. Preferably, the pharmaceutical compositions release less than about 80 weight percent of the active agent in the first eight hours after ingestion of the composition, with the balance of the pharmaceutically active agent being released thereafter. In preferred compositions, less than about 15 weight percent of the pharmaceutically active agent is released in the first 0.5 hour after ingestion, from about 10 to about 50 weight percent of the pharmaceutically active agent is released within about 2 hours after ingestion, and from about 40 to about 60 weight percent of the pharmaceutically active agent is released within about 6 hours after ingestion.
The pharmaceutical compositions comprise from about 15 to about 50 weight percent of HPMC, preferably from about 20 to about 40 weight percent of HPMC, based on total weight of the composition. The HPMC and the non-ionic, hydrophilic polymer preferably are present at a weight ratio of HPMC to non-ionic, hydrophilic polymer ranging from about 10:1 to about 3:1, more preferably from about 7:1 to about 5:1, and even more preferably about 6:1.
One HPMC polymer useful in the pharmaceutical composition described herein is available commercially from Dow Chemical under the trade name METHOCEL .
Preferably, the HPMC will have a hydroxypropyl (HP) degree of substitution up to about 12, i.e., the HPMC
will comprise up to about 12 percent HP functionality. Preferably, the HPMC
will comprise from about 7 to about 12 percent HP functionality, and more preferably from about 7 to about 9 percent HP. The HPMC preferably will have normal viscosity (2.0% HPMC in water) of from about 100 to about 100,000 cps and a number average molecular weiglit of about 20,000 to about 170,000. A particularly preferred HPMC is METHOCEL KIOOLV, which has a number average molecular weight of about 20,000 to about 30,000. Methods of making such HPMC
polymers are well known by those skilled in the art.

Upon ingestion, the non-ionic, hydrophilic polymer and the HPMC form a gel matrix in which the active agent is contained. The pharmaceutically active agent is then released from the gel matrix over time, thereby providing sustained-release of the active agent, such that a substantial amount of the released active agent may be processed efficiently at the targeted active site. Preferably, the gel matrix has sufficient strength to prevent substantial premature degradation of the matrix. The gel matrix should also be formed within a time period that is effective to prevent the premature release of the active agent prior to formation of the gel matrix.
For example, the gel matrix preferably forms within about 5 minutes after ingestion of the composition to prevent a burst of active agent prior to gel formation. It is believed that the nonionic, hydrophilic polymer operates to decrease the rate of gel formation to an acceptable level.
Typical pharmaceutically active agents which may be administered via the instant invention include, but are not limited to: (a) central nervous system (CNS) agents, such as antipsychotics, anticonvulsants, including carbamazepine and oxcarbazepine, antidepressants, antiepileptics, anxiolytics, and hypnotics; (b) cardiovascular agents, such as anti-arrhythmics, hypolipedemics, anti-anginals, anti-coagulants, anti-hypertensives, antiplatelets, diuretics, and electrolytes (Ca, K, Mg); and (c) antiinflammatories, antiasthmatics, antiarthritics, oral hypoglycemics, and aromatase inhibitors; to name a few.
The pharmaceutically active agents that can be delivered include inorganic and organic compounds without limitation, including drugs that act on the peripheral nerves, adrenergic receptors, cholinergic receptors, nervous system, skeletal muscles, cardiovascular, smooth muscles, blood circulatory system, synaptic sites, neuroeffector junctional sites, endocrine and hormone systems, immunological system, reproductive system, skeletal system, alimentary and excretory systems, inhibitory of hormonal and histamine systems, those materials that act on the central nervous system, such as antidepressants, including amiflamine, amitriptyline, alaproclate, protriptyline, doxepin, imiprimine, trazodine, paprotiline, zimelidine, fluvoxamine; antipsychotic-neuroleptic agents such as chlorpromazine, haloperidol, thioridazine, trifluoperazine, MK-0212, remoxipride; anticonvulsants, such as carbamazepine, oxcarbamazepine, phenytoin, phenobarbital; sedative-hypnotic agents, such as triazolam, clllordiazepoxide, temazepam, chlorazepate, alprazolam, diazepam, flurazepam, lorazepam, oxazepam, hydroxyzine, prazepam, meprobamate, butalbital, orphenadrine, chlorzoxazone, cyclobenzaprine;
antiparkinson agents, such as benztropine, carbidopa, levodopa, L 647,339; analgesics, such as acetaminoplien, oxycodone, hydrocodone, codeine, and propoxyphen. Respiratory agents, including sympathomimetics, brochodilators, antihistamines; and antiasthmatics, such as diethylpropion, ephedrine, epinephrine, isoproterenol, metaproterenol, terbutaline, cyproheptadine, azatadine, diphenhydramine, promethazine, chlorpheniramine, brompheniramine, aminophylline, theophylline, albuterol, tranilast, enprofylline, and budesonide, also may be used. -Cardiovascular and antihypertensive agents, including coronary vasodilators, cardiac glycosides, betablockers, slow calcium channel blockers, antiarrhythmics, peripheral vasodilators such as isosorbide dinitrate, nitroglycerin, dipyridamole, digoxin, nadolol, propranolol, metaprolol, atenolol, timolol, disopyramide, procainamide, nifedipine, quinidine, lidocaine, diltiazam, verapamil, prazosin, clinidine, hydralazine, methyldopa, captopril, metyresine, enalapril, lysinopril, felodipine, tocainide, also may be used. Diuretics, such as amiloride, spiranolactone, hydrochlorothiazide, clilorothiazide, acetazolamide, chlorthalidone, metolazone, furosemide, triamterene, methyclothiazide, ethacrynic acid, indacrinone; antiartereosclerotic agents, such as conjugated estrogens, estradiol, ethinyl estradiol, diethylstilbesterol; progestins, such as progesterone, hydroxyprogesterone, medroxyprogesterone, norethindrone; glucocorticoids and mineralocorticoids, such as hydrocortisone, betametliasone, dexamethasone, metliylprednisolone, prednisolone, prednisone, triamcinolone, and MK-0621, also may be used.
Nonsteroidal anti-inflammatory agents, antiarthritic and antigout agents, such as allopurinol, aspirin, fenprofen, ibuprofen, indomethacin, naproxen, phenylbutazone, sulindac, tolmetin, diflunisol, piroxicam, mectofenamate, penicillamine, probenecid, and coichicine; gastrointestinal agents, including anticholinergics, antispasmodics, antidiarrheal; and antiulcer histamine-H2-antagonists, such as bethanechol, clidiniutn, dicyclomine, meclizine, prochlorperazine, trimetllobenzamide, loperamide, cimetadine, ranitidine, diphenoxylate, famotidine, and omeprazole;
oral hypoglycemics, such as chlorpropamide tolazamide and tolbutamide;
anticoagulants, such as warfarin, phenindione, and anisindione; anti-infective agents, including antibiotic, antimicrobial, antiviral, antiparasitic; and antifungal agents, such as cefoxitin, thiabendazole, cephalexin, tetracycline, ampicillin, amoxicillin, sulfamethoxacole, cefaclor, erytliromycin, penicillin, nitrofurantoin, minocycline, doxycycline, cefadroxil, miconazole, phenazopyridine, norfloxacin, clorsulon, fludalanine, pentizidone, cilastin, phosplionomycin, ivermectin, imipenem, arprinocid, and foscarnet; nutritional supplements, including vitamins such as isotretinion (Vit. A), Vit. D, tocoplierols (Vit. E), and pliytonadione (Vit. K); amino acids, such as L-tryptophan and L-lysine;
aiid lipids, such as corn oil and medium chain triglycerides, also may be used. Another class of pliarmaceutical agents which may be used include those agents which aid in the reduction of cholesterol in humans.

'WO 00/21525 PCT/EP99/07627 The pharmaceutically active agents previously listed may be present in the pharmaceutical composition in an amount ranging from about 0.1 to about 80 weight percent, preferably about 10 to about 50 weight percent, more preferably about 20 to about 40 weight percent.
A class of pharmaceutically active agents known as HMG-CoA reductase inhibitors are known for use in certain pharmaceutical compositions to enhance the lowering of plasma cholesterol level in humans. Methods of making the HMG-CoA reductase inhibitors are well known by those skilled in the art and such agents include those commercially available as fluvastatin (available from Novartis Pharmaceuticals, Inc. under the trade name LESCOL ), simvastatin (available from Merck & Co., Inc. under the trade name ZOCORO), atorvastatin (available from Warner-Lambert under the trade name LIPITORO), pravastatin (available from Bristol-Myer Squibb under the trade name PRAVACHOL ), cerivastatin (available from BASF
under the trade name LIPOBAYO), lovastatin (available froin Merck & Co., Inc.
under the trade name MEVACORO) and mevastatin. The HMG-CoA reductase inhibitors may be used in their free acid forms, in their ester forms, or as their pharmaceutically acceptable salts. Such pharmaceutically acceptable salts include, for example, sodium salts, calcium salts, and ester salts.
The HMG-CoA reductase inhibitors may be used as racemic mixtures, or as a more active stereoisomer as appropriate. For example, a racemic mixture of 3-R-5-S-fluvastatin sodium and 3-S-5-R fluvastatin sodium may be used, although the stereoisomer 3-fluvastatin sodium has been found to be the more active form.
The HMG-CoA reductase inhibitors may be present in an amount effective to inhibit biosyiithesis of etiolesterol in humans. In one embodiment, the pliarmaceutical compositions comprise from about 5 to about 50 weight percent of the HMG-CoA reductase inhibitor, based on total weiglit of the composition. More preferably, the compositions comprise from about 20 to about 40 weigllt percent of the HMG-CoA reductase inhibitors, based on total weight of the composition.
Other ingredients which may be incorporated into the conipositions to facilitate processing and/or provide enhanced properties of the composition include well-known tableting binders (e.g., gelatin, sugars, natural and synthetic gums, polyvinylpyrrolidone), disintegrants (e.g., croscarmelose, crospovidone, sodium starch glycolate), lubricants (e.g., magnesium stearate, hydrogenated vegetable oil, carnauba wax); flow agents (e.g., silicon dioxide), anti-adherents or glidants (e.g., talc) as well as sweeteners, coloring niediums (e.g., iron oxide, aluminum flakes), filler materials (e.g., lactose and other carbohydrates, pregelitinized starch, potassium bicarbonate), flavoring mediums, and antioxidants. Selection of a particular ingredient or combinations of ingredients and the amounts used will be readily determinable by one skilled in the art by reference to standard procedures and practices for preparing tableted or encapsulated or other dosage forms.
The pharmaceutical compositions described herein may be administered to mammals, more particularly humans, as treatments associated with the particular pharmaceutically active agents included therein.

Example 1 A portion of fluvastatin sodium is calculated and weighed. Potassium bicarbonate, microcrystalline cellulose, povidone, HPC, and HPMC are weighed and placed into individual separately labeled containers. A 20 weight percent excess of the batch quantity of OPADRYO
Yellow, YS-1-6347-G, is then placed into a labeled container. The microcrystalline cellulose, fluvastatin sodium, povidone, HPC, and HPMC are transferred, in that order, into a collette gral and mixed for 5 minutes with the plow at slow speed and the chopper off. The resulting mixture is passed througll a 0.033 inch screen using a tornado mill with knives forward and at a slow speed. The screened material is then mixed again in a collette grat with the plow at slow speed and the chopper off.
Potassium bicarbonate is dissolved into purified water until a clear homogenous solution is obtained. The potassium bicarbonate solution is then combined with the screened material, and the resulting mixture is granulated in a collette gral with the plow at fast speed and the chopper at slow speed. After adding the above solution, granulation sliould continue for 30 seconds with the plow at fast speed and the cliopper at slow speed and for anotlier 30 seconds with the plow at fast speed and the chopper at fast speed. The granulated mixture is then dried in a fluid bed dryer using a target inlet temperature of 50 degrees C until an LOD of 2 percent to 3 percent is obtained.
The dried granules are then passed througlia 1/16 inch screen using a tornado mill with knives forward and at slow speed. An amount of magnesiuin stearate based on the proportion of actual yield from the 1/16 inch screening step to the theoretical yield from the same step is calculated and weighed. The weiglied magnesiuni stearate is then passed througli a 60 mesh screen and blended with the dried granules in a free fall blender and the resulting granulation blend discliarged into a plastic lined labeled drum. The granulation blend is then compressed into tablets and the tablets are dedusted, passed through a metal checker, and stored in a plastic, labeled drum.
To coat the tablets, the OPADRY Yellow is mixed with a required quantity of purified water to obtain a 10 w/w percent suspension. The tablets are transferred to a coating pan and warmed to a temperature of 40-45 degrees C. The OPADRY Yellow suspension is then added, to spray coat the tablets until a 3 percent solid weight gain per tablet is achieved. The coating spray is shut off, and the tablets are cooled by shutting off the pan heat and jogging the pan for 5 minutes.

Example 2 84.24 mg of fluvastatin sodium were combined with the following excipients according to the method described in Example I to provide a single dosage form described in Table 1:

Fluvastatin sodium 84.24 mg Potassium bicarbonate, USP 8.42 mg Microcrystalline cellulose, NF, PH101 111.26 mg (AVICEL ).
Povidone,USP 4.88 mg HPC,NF (KLUCEL HXF) 16.25 mg HPMC, USP (METHOCEL K 100LV) 97.50 mg Magnesium Stearate 2.44 mg OPADRY Yellow 9.75 mg Example 3 84.25 mg of fluvastatin sodium were combined with the following excipients by the method described in Example I to provide a single dosage form described in Table 2:

Fluvastatin sodium 84.25 mg Potassium bicarbonate, USP 8.42 mg Microcrystalline cellulose, NF, PH101 111.2 mg (AVICEL ) Povidone, USP 4.88 mg HPC, HF (KLUCEL HXF) 16.25 mg HPMC, USP (METHOCEL K IOOLV) 32.50 mg HPMC, USP (METHOCEL K15M) 32.50 mg HPMC, USP (METHOCEL K4M) 32.50 mg Magnesium Stearate, NF 2.44 mg OPADRYO Yellow, YS-1-6347-G 9.75 mg Example 4 168.48 mg of fluvastatin sodium were combined with the following excipients according to the method described in Example 1 to provide a single dosage form described in Table 3:

Fluvastatin sodium 168.48 mg Potassium bicarbonate, USP 8.42 mg Microcrystalline cellulose, NF, PH101 65 mg (AVICEL ) Povidone, USP 20.5 mg HPC, NF (KLUCEL HXF) 20.5 mg HPMC,USP (METHOCEL K 100LV) 110.7 mg HPMC, USP (METHOCEL ) 12.3 mg Magnesium stearate, NF (1%) 4.1 mg OPADRYO Red 12.3 mg Example 5 Dosage forms of the pharmaceutical composition described in Example 2 were prepared, while varying the weight percentage of HPMC from 30 weight percent to 10 weight percent in 5 weight percent increments. Each dosage form was then tested for its dissolution in water while stirring at a paddle speed of 50 rpm, at a temperature of 37 C.
The results of each experiment were plotted in a graph as percentage dissolution versus time as shown in Figure 1.

Comparative Example 1 A dosage form having the composition described below in Table 4 was prepared according to the method described in Example 1:

Fluvastatin sodium 42.12 mg Sodium bicarbonate 4.21 mg Microcrystalline cellulose, NF (PH01) 146.17 mg Povidone 6.25 mg HPC, NF (KLUCEL HXF) 50.00 mg Magnesium stearate NF 1.25 mg OPADRY Yellow 10.00 mg Comparative Example 2 A dosage form liaving the composition sliown below in Table 5 was prepared according to the metliod described in Example 1:

Fluvastatin sodium 42.12 mg Sodium bicarbonate 4.21 mg Microcrystalline cellulose, NF (PH01) 118.67 mg Povidone 6.25 mg HPMC, NF (METHOCEL HXF) 77.50 mg Magnesium stearate NF 1.25 mg OPADRY Yellow 10.00 mg Comparative Example 3 The dosage forms of Example 2, Comparative Example 1, and Comparative Example were tested for their dissolution at a temperature of 37 C by placing each dosage form in 100 mM acetate buffer and stirring at a paddle speed of 50 rpm.
The acetate buffer contained 4.0 grams of sodium hydroxide dissolved in about milliliters of water. The pH was adjusted to 4.0 by the addition of acetic acid, and the solution was diluted to one liter with distilled water.
The dissolution data were plotted in a graph as percentage dissolution versus time as shown in Figure 2. As can be seen from the plot, the fluvastatin composition of Comparative Example I containing HPC but no HPMC showed an undesirably high rate of dissolution as compared to the composition of Example 2.

Comparative Example 4 The dosage forms of Example 2, Comparative Example 1, and Comparative Example were tested for their dissolution at a temperature of 37 C by placing each dosage form in 50 mM
phosphate buffer, pH 6.8, and stirring at paddle speeds of 50 rpm and 100 rpm.
The phosphate buffer contained 3.312 grams of monobasic sodium phosphate monohydrate and 3.692 grams of dibasic sodium phosphate anliydrous dissolved in about 500 milliliters of water. The resulting solution was diluted to one liter with distilled water.
The dissolution data were plotted in a graph as percentage dissolution versus time as shown in Figure 3. As can be seen from the plot, the fluvastatin composition of Example 2 sliowed a release profile comparable at a stirring speed of 50 rpm to the fluvastatin compositions having only one of HPMC or HPC.

Comparative Example 5 Tiie dosage forms of Example 2, Comparative Example 1, and Comparative Example were tested for their dissolution at a temperature of 37 C by placing each dosage form distilled water and stirring at a paddle speed of 50 rpm.
The results of eacli experiment were plotted in a grapli as percentage dissolution versus tinie as shown in Figure 4. As can be seen from the plot, the fluvastatin composition of Example 2 showed a dissolution profile comparable to the fluvastatin compositions liaving only one of HPMC or HPC.

Comparative Example 6 The dosage forms of Example 2 and Comparative Example 2 were repeatedly tested for their dissolution at a temperature of 37 C by placing each dosage form in 50 mM pliosphate buffer, pH 6.8, and stirring at a paddle speed of 50 rpm.
The phosphate buffer contained 3.312 grams of monobasic sodium phosphate monoliydrate and 3.692 grams of dibasic sodium phosphate anhydrous dissolved in about 500 milliliters of water. The resulting solution was diluted to one liter with distilled water.

Dissolution data for Example 2 and Comparative Example 2 were plotted on a graph as percentage dissolution versus time as shown in Figures 5 and 6, respectively.
Each of Figures 5 and 6 shows multiple repetitions of the same dissolution tests with each repetition shown with a separate set of uniquely marked data points (for example, stars, asterisks, crosses, boxes and circles), with each set of data points joined to form a curve of percent released vs. time (hours). A
comparison of Figures 5 and 6 show that the composition of Example 2, containing both HPMC and HPC, showed better reproducibility in its dissolution profile than the composition of Comparative Example 2, which contained only HPMC.

Claims (17)

CLAIMS:

1. A pharmaceutical composition, comprising:

fluvastatin or a pharmaceutically acceptable salt thereof, hydroxypropyl methyl cellulose; and a non-ionic, hydrophilic polymer selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 90,000 to 1,300,000, hydroxypropyl cellulose having a number average molecular weight of 370,000 to 1,500,000 and poly(ethylene oxide) having a number average molecular weight ranging from 100,000 to 500,000.

2. A pharmaceutical composition of claim 1, wherein the non-ionic, hydrophilic polymer is selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 1,000,000 to 1,300,000, hydroxypropyl cellulose having a number average molecular weight of 850,000 to 1,500,000, and poly(ethylene oxide) having a number average molecular weight ranging from 150,000 to 300,000.

3. A pharmaceutical composition of claim 1, wherein the non-ionic, hydrophilic polymer is hydroxypropyl cellulose having a number average molecular weight of about 1,150,000.

4. A pharmaceutical composition of any one of claims 1 to 3, comprising from about 5 to about 50 weight percent of the fluvastatin or the pharmaceutically acceptable salt thereof, based on total weight of said composition.

5. A pharmaceutical composition of any one of claims 1 to 3, comprising from about 20 to about 40 weight percent of the fluvastatin or the pharmaceutically acceptable salt thereof, based on total weight of said composition.

6. A pharmaceutical composition of any one of claims 1 to 5, comprising from about 15 to about 50 weight percent of said hydroxypropyl methyl cellulose, based on total weight of said composition.

7. A pharmaceutical composition of any one of claims 1 to 5, comprising from about 20 to about 40 weight percent of said hydroxypropyl methyl cellulose, based on total weight of said composition.

8. A pharmaceutical composition of any one of claims 1 to 7, comprising from about 3 to about 12 weight percent of said non-ionic hydrophilic polymer, based on total weight of said composition.

9. A pharmaceutical composition of any one of claims 1 to 7, comprising from about 4 to about 7 weight percent of said non-ionic hydrophilic polymer, based on total weight of said composition.

10. A pharmaceutical composition of any one of claims 1 to 9, wherein the weight ratio of said hydroxypropyl methyl cellulose to said non-ionic hydrophilic polymer ranges from about 10:1 to about 3:1.

11. A pharmaceutical composition of any one of claims 1 to 9, wherein the weight ratio of said hydroxypropyl methyl cellulose to said non-ionic hydrophilic polymer is about 7:1 to about 5:1.

12. Use of hydroxypropyl methyl cellulose and a non-ionic, hydrophilic polymer in preparation of a pharmaceutical composition comprising fluvastatin or a pharmaceutically acceptable salt thereof, wherein the pharmaceutical composition is for sustained release of the fluvastatin or the pharmaceutically acceptable salt thereof and wherein the non-ionic, hydrophilic polymer is selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 90,000 to 1,300,000, hydroxypropyl cellulose having a number average molecular weight of 370,000 to 1,500,000 and poly(ethylene oxide) having a number average molecular weight ranging from 100,000 to 500,000.

13. Use of claim 12, wherein the non-ionic, hydrophilic polymer is selected from the group consisting of hydroxyethyl cellulose having a number average molecular weight ranging from 1,000,000 to 1,300,000, hydroxypropyl cellulose having a number average molecular weight of 850,000 to 1,500,000, and poly(ethylene oxide) having a number average molecular weight ranging from 150,000 to 300,000.

14. Use of claim 12, wherein the non-ionic, hydrophilic polymer is hydroxypropyl cellulose having a number average molecular weight of about 1,150,000.

15. Use of any one of claims 12 to 14, wherein the pharmaceutical composition comprises from about to about 50 weight percent of the fluvastatin or the pharmaceutically acceptable salt thereof, based on total weight of said composition.

16. Use of any one of claims 12 to 15, wherein the pharmaceutical composition comprises from about 15 to about 50 weight percent of said hydroxypropyl methyl cellulose, based on total weight of said composition.

17. Use of any one of claims 12 to 16, wherein the pharmaceutical composition comprises from about 3 to about 12 weight percent of said non-ionic hydrophilic polymer, based on total weight of said composition.