CA2236003C - Process for making crystalline iron dextran - Google Patents
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Abstract
A process for the crystallization of an iron-dextran complex in readily-filterable form comprising adding slowly, with stirring, an acidified aqueous iron-dextran solution to a vessel containing a water miscible organic solvent such as methanol, as well as crystalline iron-dextran complexes so prepared.
Description
PROCESS FOR TAKING CRYSTAL~IaINE IRON DF.XTRAN
TP.e n'cal Field The present invention relates to a process for making iron preparations useful in the s treatment of iron deficiencies. More particularly, the invention relates to a process for the preparation of crystalline iron(III)-dextran complex.
ack~round ~f th_P Tnvention Iron dextran has been known for about 35 years. It has been utilized primarily as an 1 o injectable agent for countering iron deficiencies in animals and human patients, having severa advantages over other iron preparations including low toxicity, low incidence of adverse reactions, and satisfactory rate of iron adsorption.
Iron dextran is typically prepared by forming a complex of dextran with iron(III). The dextrans used are generally either (i) partially depolymerized dextrans having a molecular i s weight in the range of 1,000 to 10,000 or (ii) modified forms of dextran such as hydrogenated dextrans or oxidized dextrans. The iron(TII) is prepared by neutralization of an aqueous solution of an iron(III) salt with an alkali. In order to prepare an aqueous iron dextran solution in a form suitable for injection, it is necessary to remove the soluble salts formed in the reaction between the water-soluble ferric salt and the dextran. This can be accomplished by known 2o procedures such as dialysis or reverse osmosis, or by precipitation of the iron dextran complex from the aqueous solution, followed by drying and reconstitution with water.
Isolation of the iron-dextran complex from the aqueous solution by precipitation with a water-miscible solvent is known in the art. United States Patent No. 4,599,405 describes centrifuging and filtering the aqueous iron-dextran solution, followed by precipitation of the 2s complex with ethanol. The complex is then washed with ethanol, separated, and dried to give the iron-dextran complex as a solid which is then reconstituted with water to form an inject;ible solution.
United States Patent Number 3,093,545 describes isolation of a treacle-like solid iron dextran complex by addition of methanol to the aqueous iron dextran solution, allowing thc~
3o solid to settle, and decanting the supernatant liquid. The residual solid is then washed with aqueous ethanol, the liquid is decanted, and the process repeated. The residual liquid is removed by vacuum filtration, and the remaining solid complex is dried.
United States Patent Number 3,574,184 describes isolation of a treacle-like solid iron dextran complex by addition of ethanol to the aqueous solution and one or more centrifugation 35 Steps.
United States Patent Number 2,885,393 describes a process for making a gelatinous form of an iron dextxan complex wherein the complex is purified by precipitation from aqueous solution by addition of isopropanol, followed by decanting the supernatant liquid, redissolving the solid in water, and repeating the process. After further processing, the iron dextran complex is obtained as an aqueous solution.
United States Re. 24,642 describes purification of the iron-dextran complex by filtering the aqueous solution, precipitating the complex by mixing with ethanol, filtering the gelatinous s solid., and redissolving the complex in water and repeating the precipitation with ethanol. The , iron-dextran complex is then dissolved in water to form the injectable solution.
All of the prior art methods for isolating the solid iron-dextran complex require addition of the precipitating solvent to the aqueous iron dextran solution and result in gelatinous or treacle-like solids which are difficult to filter. Alternate methods of solvent removal, such as 1 o centrifugation or decantation are therefore required. Substantial further processing of the initial solid is also required to obtain complex of sufficient purity for use as injecta.ble solutions.
Consequently, there remains a need for a method of precipitation which produces pure iron dextran complex which can be easily isolated by filtration and dried. The dry powder can then be stored and reconstituted as needed to form the aqueous solution, thereby obviating storage i s difficulties which result from the prior art methods in which the complex is prepared and/or stored as a 5-to-10°~o aqueous solution.
Summary of t_he Inv n ,nn It has now been found that a process in which an iron-dextran complex is added to a 2o solvent, instead of the solvent being added to the iron-dextran complex, unexpectedly results in the formation of an easily-filtered crystalline solid which can be dried to a powder and easily stored for later reconstitution with water. This ease of filtration, and the elimination of the need for reverse osmosis, decanting, or other means of isolation of the solid from the residual aqueous solution, offer a means of considerable cost savings.
2s Accordingly, in one aspect of the present invention is disclosed a method of making crystalline iron-dextran complex, comprising the steps of:
(a) combining an aqueous solution of dextran in which the dextran has an average molecular of 3,000 to 6,000 with an aqueous solution of an iron(III) salt;
(b) combining the mixture with alkali;
30 (c) heating and stirring the mixture until the formation of an iron-dextran complex is substantially complete;
(d) acidifying the mixture;
(e) precipitating the iron-dextran complex by adding the mixture to a water miscible solvent; and ss (f) isolating the crystalline iron-dextran complex.
TP.e n'cal Field The present invention relates to a process for making iron preparations useful in the s treatment of iron deficiencies. More particularly, the invention relates to a process for the preparation of crystalline iron(III)-dextran complex.
ack~round ~f th_P Tnvention Iron dextran has been known for about 35 years. It has been utilized primarily as an 1 o injectable agent for countering iron deficiencies in animals and human patients, having severa advantages over other iron preparations including low toxicity, low incidence of adverse reactions, and satisfactory rate of iron adsorption.
Iron dextran is typically prepared by forming a complex of dextran with iron(III). The dextrans used are generally either (i) partially depolymerized dextrans having a molecular i s weight in the range of 1,000 to 10,000 or (ii) modified forms of dextran such as hydrogenated dextrans or oxidized dextrans. The iron(TII) is prepared by neutralization of an aqueous solution of an iron(III) salt with an alkali. In order to prepare an aqueous iron dextran solution in a form suitable for injection, it is necessary to remove the soluble salts formed in the reaction between the water-soluble ferric salt and the dextran. This can be accomplished by known 2o procedures such as dialysis or reverse osmosis, or by precipitation of the iron dextran complex from the aqueous solution, followed by drying and reconstitution with water.
Isolation of the iron-dextran complex from the aqueous solution by precipitation with a water-miscible solvent is known in the art. United States Patent No. 4,599,405 describes centrifuging and filtering the aqueous iron-dextran solution, followed by precipitation of the 2s complex with ethanol. The complex is then washed with ethanol, separated, and dried to give the iron-dextran complex as a solid which is then reconstituted with water to form an inject;ible solution.
United States Patent Number 3,093,545 describes isolation of a treacle-like solid iron dextran complex by addition of methanol to the aqueous iron dextran solution, allowing thc~
3o solid to settle, and decanting the supernatant liquid. The residual solid is then washed with aqueous ethanol, the liquid is decanted, and the process repeated. The residual liquid is removed by vacuum filtration, and the remaining solid complex is dried.
United States Patent Number 3,574,184 describes isolation of a treacle-like solid iron dextran complex by addition of ethanol to the aqueous solution and one or more centrifugation 35 Steps.
United States Patent Number 2,885,393 describes a process for making a gelatinous form of an iron dextxan complex wherein the complex is purified by precipitation from aqueous solution by addition of isopropanol, followed by decanting the supernatant liquid, redissolving the solid in water, and repeating the process. After further processing, the iron dextran complex is obtained as an aqueous solution.
United States Re. 24,642 describes purification of the iron-dextran complex by filtering the aqueous solution, precipitating the complex by mixing with ethanol, filtering the gelatinous s solid., and redissolving the complex in water and repeating the precipitation with ethanol. The , iron-dextran complex is then dissolved in water to form the injectable solution.
All of the prior art methods for isolating the solid iron-dextran complex require addition of the precipitating solvent to the aqueous iron dextran solution and result in gelatinous or treacle-like solids which are difficult to filter. Alternate methods of solvent removal, such as 1 o centrifugation or decantation are therefore required. Substantial further processing of the initial solid is also required to obtain complex of sufficient purity for use as injecta.ble solutions.
Consequently, there remains a need for a method of precipitation which produces pure iron dextran complex which can be easily isolated by filtration and dried. The dry powder can then be stored and reconstituted as needed to form the aqueous solution, thereby obviating storage i s difficulties which result from the prior art methods in which the complex is prepared and/or stored as a 5-to-10°~o aqueous solution.
Summary of t_he Inv n ,nn It has now been found that a process in which an iron-dextran complex is added to a 2o solvent, instead of the solvent being added to the iron-dextran complex, unexpectedly results in the formation of an easily-filtered crystalline solid which can be dried to a powder and easily stored for later reconstitution with water. This ease of filtration, and the elimination of the need for reverse osmosis, decanting, or other means of isolation of the solid from the residual aqueous solution, offer a means of considerable cost savings.
2s Accordingly, in one aspect of the present invention is disclosed a method of making crystalline iron-dextran complex, comprising the steps of:
(a) combining an aqueous solution of dextran in which the dextran has an average molecular of 3,000 to 6,000 with an aqueous solution of an iron(III) salt;
(b) combining the mixture with alkali;
30 (c) heating and stirring the mixture until the formation of an iron-dextran complex is substantially complete;
(d) acidifying the mixture;
(e) precipitating the iron-dextran complex by adding the mixture to a water miscible solvent; and ss (f) isolating the crystalline iron-dextran complex.
In another aspect of the present invention is disclosed a process for the crystallization of an iron-dextran complex in readily-filterable form, comprising adding slowly, with stirring, ;an aqueous iron-dextran solution to a vessel already containing a water miscible organic solvent..
In a further aspect of the present invention are disclosed crystalline iron-dextran complexes prepared by the above processes.
Detailed Description of the Invention Dextran is a polysaccharide polymer of formula (C6H1p05)n composed exclusively of a-D-gluconpyranosyl units linked predominately a-1-6 and obtained by the action of bacteria 1 o grown on a sucrose substrate by methods well-known in the art. Native dextrans usually have high molecular weigh>~ Lower molecular weight dextrans are prepared by partial depolymerization of the native dextrans by methods known in the art, such as treatment with mineral acid.
A dextran obtained by partial depolymerization of such a native dextran may be further i s modified, as for example by partial reduction using sodium borohydride or catalytic hydrogenation, or by oxidation. The term "dextran" as used herein is intended to include dextran, reduced dextran, and oxidized dextran. Treatment of unmodified dextran with mild oxidizing agents such as sodium or potassium periodate introduces carboxyl groups on the terminal glucose unit. Optionally, the terminal glucose unit may be oxidized to carboxylic 2o groups using, for example, bromine, sodium hypobromite, sodium bromite, sodium hypochlorite or sodium chlorite. In one embodiment of the invention, dextran is dissolved in water and oxidized by treatment with between about 0.4 and about 0.6 parts per weight (ppv~r) 5°1o aqueous sodium hypochlorite, followed by between about 0.10 and about 0.15 ppw of aqueous sodium hydroxide. The oxidation is best earned out at ambient temperature (about 15 2s to about 35 °C). A representative dextran contemplated for use in the present invention is a partially depolymerized dextran having a molecular weight of between about 1,000 and about 10,000, preferably between about 3,000 and about 6,000.
In accordance with the foregoing, between about 1 and about 10 ppw of such a dextran are dissolved in water (preferred dextrans being unoxidized or oxidized dextrans and oxidized 3 o dextrans being most preferred). A particularly preferred embodiment of the process of the present invention utilizes between about 1.0 and about 1.5 ppw of oxidized dextran having a.
molecular weight of 5,000 to 6,000. The aqueous dextran solution is then added with stirring to an aqueous solution of about 1 ppw of iron(I117, where the term "iron(III)"
means trivalent hydrous ferric oxide. The iron(IZI) solution is prepared by neutralizing a ferric salt with alkali.
ss Suitable ferric salts include any water-soluble salts which generate hydrous fernc oxide when treated with alkalis. Representative salts include fernc chloride, ferric nitrate, ferric sulfate, WO 97/17377 PC'r/LTS96/18018 ferric perchlorate, ferric acetate, fernc trichloroacetate, ferric citrate and the like, as well as double salts such as ferric ammonium sulfate or fernc ammonium citrate and the like. Of the above salts, ferric chloride is particularly preferred. Representative alkalis include the hyroxides and carbonates of sodium, lithium and potassium. The ferric salt may be neutralized by treatment with excess alkali, or may be formed in a two step process involving partial neutralization with alkali followed by addition of the dextran solution and additional alkali to achieve complete neutralization and complex formation. The iron( solution is preferably prepared by adding between about 0.14 and about 0.24 ppw of aqueous sodium carbonate to an aqueous solution containing about 1 ppw of hydrated fernc chloride.
t o After combining the aqueous iron(III) and dextran solutions, the mixture is made basic by addition of between about 0.15 and about 0.25 ppw alkali, preferably sodium hydroxide.
The mixture is then heated, preferably at between about 80 and about 100 °C, and stirred until complex formation is complete. When unoxidized dextran is used, gradual heating may be required to prevent formation of a heterogeneous mixture. The solution may then be acidified i s using solid, gaseous or liquid acid, preferably aqueous hydrochloric acid, to obtain a pH of between about 4 and about S. The solution may then be heated, preferably at between about 118 and about 135 °C, at a pressure of between about 10 and about 20 psi (68 and 138 KPa).
After cooling to ambient temperature, crystalline iron-dextran complex is obtained by adding the reaction solution to a water-miscible organic solvent in a solution-to-solvent ratio of about not 20 less than 1:l by volume, followed by filtration. Representative solvents include methanol, ethanol, isopropanol, acetone and the Like, with methanol being the most preferred solvent and (when methanol is used) the preferred ratio of aqueous iron-dextran solution to solvent being about 1:8. To reduce solvent volume, the aqueous iron-dextran solution may be concentrated prior to addition to the solvent. The initially obtained crystalline iron-dextran may be washed 2s with additional solvent prior to drying and storage.
The process of the present invention will be better understood in connection with the following Examples, which are intended solely as an illustration thereof.
Example 1 30 ~paration of ('r~stalline Iron-Dextran Complex usin~Oxidized Dextran To a solution of molecular weight 5000 Dextran (60.7 g) in water (I08 mL) was added bleach (32 mL) and S% aqueous sodium hydroxide (7.5 mL). The solution was stirred for one hour and then was refrigerated for 17 hours. To a stirred solution of ferric chloride (60 g) in s5 water (300 mL) was added, dropwise over 60 minutes, a solution of sodium carbonate (11.8 g) in water (100 mL), followed by the bleach-dextran solution. To the resulting solution was added a solution of sodium hydroxide (22.6 g) in water (100 mL) over a 60 minute period. '7Che mixture was heated at 90-100 °C for 90 minutes and then was cooled to ambient temperature and acidified with to pH 4 with HCl to give 907 g of iron-dextran solution. A
447 g aliquot was removed and concentrated to a volume of 160 mL and then was added with stirring to 1038 s g of methanol. The resulting crystalline solid was collected by filtration and washed with methanol. The solid was slurried in methanol (173 g), filtered, and dried to afford 15.63 g of iron-dextran powder. The material passed gel permeation chromatographic analysis.
>?:enaration of Crystalline Iron Dextran Complex using Unoxidized Dextran To a solution in water (70 mL) of fernc chloride (24.0 g) was added a solution of sodium carbonate (4.7 g) in water (40 mL) over an 80-minute period. To the mixture was added a solution of dextran (27.3 g) in water (70 mL), followed by addition, over 40 minutes, is of a solution of sodium hydroxide (9.0 g) in water (110 mL). The mixture was then added t~o methanol (350 mL). The resulting solid was filtered, dissolved in water (150 mL) and heated at 85 °C for 60 minutes. The mixture was cooled and added to methanol ( 100 mL). The resulting solid was filtered and dried to afford 19.4 g of iron dextran powder. The material passed gel permeation chromatographic analysis.
example 3 P~paration of Crystalline Iron Dextran Complex with fn Situ Oxidation of Dextran To a solution of molecular weight 5000 dextran (27.3 g) in water (70 mL) was added 2s 2.25 N aqueous sodium hydroxide (5 mL); the resulting mixture was heated at 75-80 °C for 30 minutes_ The solution was cooled and added to a fernc solution prepared by treating a solution of ferric chloride (24.0 g) in water (70 mL) with 1.1 N aqueous sodium carbonate (40 mL).
The resulting solution was heated to 80-90 °C, cooled, and added to methanol (100 mL) witm stirnng. The resulting crystalline solid was filtered and dried to give iron-dextran powder. The s o material passed gel permeation chromatographic analysis.
Example 4 dal Preparation of Crystalline Iron-Dextran To a 30-gallon reactor containing a 25 °C solution under NZ of ferric chloride s hexahydrate (5.0 kg) in degassed water (25 kg) was added a solution of sodium carbonate (1.0 kg) in water (8.3 kg). The temperature was maintained at 20-26 °C while stirring at 30-50 rpm during the addition. In a 50-gallon reactor, dextran (5.2 kg) was dissolved in water (9.1 kg).
To the dextran solution was added 5°k aqueous sodium hypochlorite (2.9 kg) and after thorough mixing 10% aqueous sodium hydroxide solution (0.7 kg) was added. The solution i o was stirred until it did not react with KI starch paper. The oxidized dextran solution was then added to the ferric solution over 5 minutes while maintaining the temperature of the ferric solution at 23-25 °C and stirnng at 75-100 rpm. A solution of sodium hydroxide (2.0 kg) in water (8.3 kg) was added over a period of about one hour. The reaction mixture was then stirred for 90 minutes at 21-26 °C and then was warmed to 85-100 °C for 90 minutes. The i s reaction mixture was then cooled to 30-40 °C and adjusted to pH 4.3-5.0 with HCl.
The reaction mixture was then filtered and the filtrate was heated at 121-130 °C in a sealed vessel for 80-90 minutes. The solution was concentrated to a volume of about 15 L and cooled to ambient temperature. The iron-dextran solution was then slowly added to 121 kg of methanol over about 60 minutes with vigorous stirnng. A precipitate formed instantly. The 2o solid was filtered and rinsed with methanol (120 kg) and then was suspended in 64 kg of methanol. The suspension was stirred for 30 minutes. The solid was filtered and dried in vacruo at 85 °C to give 4_03 kg of iron-dextran powder.
It is understood that the foregoing detailed description and accompanying examples are merely illustrative and are not to be taken as limitations upon the scope of the invention, which 2s is defined solely by the appended claims and their equivalents. Various changes and rnodifications to the disclosed embodiments will be apparent to those skilled in the art. Such cha~lges and modifications, including without limitation those relating to the materials and/or methods of use of the invention, may be made without departing from the spirit and scope thereof.
In a further aspect of the present invention are disclosed crystalline iron-dextran complexes prepared by the above processes.
Detailed Description of the Invention Dextran is a polysaccharide polymer of formula (C6H1p05)n composed exclusively of a-D-gluconpyranosyl units linked predominately a-1-6 and obtained by the action of bacteria 1 o grown on a sucrose substrate by methods well-known in the art. Native dextrans usually have high molecular weigh>~ Lower molecular weight dextrans are prepared by partial depolymerization of the native dextrans by methods known in the art, such as treatment with mineral acid.
A dextran obtained by partial depolymerization of such a native dextran may be further i s modified, as for example by partial reduction using sodium borohydride or catalytic hydrogenation, or by oxidation. The term "dextran" as used herein is intended to include dextran, reduced dextran, and oxidized dextran. Treatment of unmodified dextran with mild oxidizing agents such as sodium or potassium periodate introduces carboxyl groups on the terminal glucose unit. Optionally, the terminal glucose unit may be oxidized to carboxylic 2o groups using, for example, bromine, sodium hypobromite, sodium bromite, sodium hypochlorite or sodium chlorite. In one embodiment of the invention, dextran is dissolved in water and oxidized by treatment with between about 0.4 and about 0.6 parts per weight (ppv~r) 5°1o aqueous sodium hypochlorite, followed by between about 0.10 and about 0.15 ppw of aqueous sodium hydroxide. The oxidation is best earned out at ambient temperature (about 15 2s to about 35 °C). A representative dextran contemplated for use in the present invention is a partially depolymerized dextran having a molecular weight of between about 1,000 and about 10,000, preferably between about 3,000 and about 6,000.
In accordance with the foregoing, between about 1 and about 10 ppw of such a dextran are dissolved in water (preferred dextrans being unoxidized or oxidized dextrans and oxidized 3 o dextrans being most preferred). A particularly preferred embodiment of the process of the present invention utilizes between about 1.0 and about 1.5 ppw of oxidized dextran having a.
molecular weight of 5,000 to 6,000. The aqueous dextran solution is then added with stirring to an aqueous solution of about 1 ppw of iron(I117, where the term "iron(III)"
means trivalent hydrous ferric oxide. The iron(IZI) solution is prepared by neutralizing a ferric salt with alkali.
ss Suitable ferric salts include any water-soluble salts which generate hydrous fernc oxide when treated with alkalis. Representative salts include fernc chloride, ferric nitrate, ferric sulfate, WO 97/17377 PC'r/LTS96/18018 ferric perchlorate, ferric acetate, fernc trichloroacetate, ferric citrate and the like, as well as double salts such as ferric ammonium sulfate or fernc ammonium citrate and the like. Of the above salts, ferric chloride is particularly preferred. Representative alkalis include the hyroxides and carbonates of sodium, lithium and potassium. The ferric salt may be neutralized by treatment with excess alkali, or may be formed in a two step process involving partial neutralization with alkali followed by addition of the dextran solution and additional alkali to achieve complete neutralization and complex formation. The iron( solution is preferably prepared by adding between about 0.14 and about 0.24 ppw of aqueous sodium carbonate to an aqueous solution containing about 1 ppw of hydrated fernc chloride.
t o After combining the aqueous iron(III) and dextran solutions, the mixture is made basic by addition of between about 0.15 and about 0.25 ppw alkali, preferably sodium hydroxide.
The mixture is then heated, preferably at between about 80 and about 100 °C, and stirred until complex formation is complete. When unoxidized dextran is used, gradual heating may be required to prevent formation of a heterogeneous mixture. The solution may then be acidified i s using solid, gaseous or liquid acid, preferably aqueous hydrochloric acid, to obtain a pH of between about 4 and about S. The solution may then be heated, preferably at between about 118 and about 135 °C, at a pressure of between about 10 and about 20 psi (68 and 138 KPa).
After cooling to ambient temperature, crystalline iron-dextran complex is obtained by adding the reaction solution to a water-miscible organic solvent in a solution-to-solvent ratio of about not 20 less than 1:l by volume, followed by filtration. Representative solvents include methanol, ethanol, isopropanol, acetone and the Like, with methanol being the most preferred solvent and (when methanol is used) the preferred ratio of aqueous iron-dextran solution to solvent being about 1:8. To reduce solvent volume, the aqueous iron-dextran solution may be concentrated prior to addition to the solvent. The initially obtained crystalline iron-dextran may be washed 2s with additional solvent prior to drying and storage.
The process of the present invention will be better understood in connection with the following Examples, which are intended solely as an illustration thereof.
Example 1 30 ~paration of ('r~stalline Iron-Dextran Complex usin~Oxidized Dextran To a solution of molecular weight 5000 Dextran (60.7 g) in water (I08 mL) was added bleach (32 mL) and S% aqueous sodium hydroxide (7.5 mL). The solution was stirred for one hour and then was refrigerated for 17 hours. To a stirred solution of ferric chloride (60 g) in s5 water (300 mL) was added, dropwise over 60 minutes, a solution of sodium carbonate (11.8 g) in water (100 mL), followed by the bleach-dextran solution. To the resulting solution was added a solution of sodium hydroxide (22.6 g) in water (100 mL) over a 60 minute period. '7Che mixture was heated at 90-100 °C for 90 minutes and then was cooled to ambient temperature and acidified with to pH 4 with HCl to give 907 g of iron-dextran solution. A
447 g aliquot was removed and concentrated to a volume of 160 mL and then was added with stirring to 1038 s g of methanol. The resulting crystalline solid was collected by filtration and washed with methanol. The solid was slurried in methanol (173 g), filtered, and dried to afford 15.63 g of iron-dextran powder. The material passed gel permeation chromatographic analysis.
>?:enaration of Crystalline Iron Dextran Complex using Unoxidized Dextran To a solution in water (70 mL) of fernc chloride (24.0 g) was added a solution of sodium carbonate (4.7 g) in water (40 mL) over an 80-minute period. To the mixture was added a solution of dextran (27.3 g) in water (70 mL), followed by addition, over 40 minutes, is of a solution of sodium hydroxide (9.0 g) in water (110 mL). The mixture was then added t~o methanol (350 mL). The resulting solid was filtered, dissolved in water (150 mL) and heated at 85 °C for 60 minutes. The mixture was cooled and added to methanol ( 100 mL). The resulting solid was filtered and dried to afford 19.4 g of iron dextran powder. The material passed gel permeation chromatographic analysis.
example 3 P~paration of Crystalline Iron Dextran Complex with fn Situ Oxidation of Dextran To a solution of molecular weight 5000 dextran (27.3 g) in water (70 mL) was added 2s 2.25 N aqueous sodium hydroxide (5 mL); the resulting mixture was heated at 75-80 °C for 30 minutes_ The solution was cooled and added to a fernc solution prepared by treating a solution of ferric chloride (24.0 g) in water (70 mL) with 1.1 N aqueous sodium carbonate (40 mL).
The resulting solution was heated to 80-90 °C, cooled, and added to methanol (100 mL) witm stirnng. The resulting crystalline solid was filtered and dried to give iron-dextran powder. The s o material passed gel permeation chromatographic analysis.
Example 4 dal Preparation of Crystalline Iron-Dextran To a 30-gallon reactor containing a 25 °C solution under NZ of ferric chloride s hexahydrate (5.0 kg) in degassed water (25 kg) was added a solution of sodium carbonate (1.0 kg) in water (8.3 kg). The temperature was maintained at 20-26 °C while stirring at 30-50 rpm during the addition. In a 50-gallon reactor, dextran (5.2 kg) was dissolved in water (9.1 kg).
To the dextran solution was added 5°k aqueous sodium hypochlorite (2.9 kg) and after thorough mixing 10% aqueous sodium hydroxide solution (0.7 kg) was added. The solution i o was stirred until it did not react with KI starch paper. The oxidized dextran solution was then added to the ferric solution over 5 minutes while maintaining the temperature of the ferric solution at 23-25 °C and stirnng at 75-100 rpm. A solution of sodium hydroxide (2.0 kg) in water (8.3 kg) was added over a period of about one hour. The reaction mixture was then stirred for 90 minutes at 21-26 °C and then was warmed to 85-100 °C for 90 minutes. The i s reaction mixture was then cooled to 30-40 °C and adjusted to pH 4.3-5.0 with HCl.
The reaction mixture was then filtered and the filtrate was heated at 121-130 °C in a sealed vessel for 80-90 minutes. The solution was concentrated to a volume of about 15 L and cooled to ambient temperature. The iron-dextran solution was then slowly added to 121 kg of methanol over about 60 minutes with vigorous stirnng. A precipitate formed instantly. The 2o solid was filtered and rinsed with methanol (120 kg) and then was suspended in 64 kg of methanol. The suspension was stirred for 30 minutes. The solid was filtered and dried in vacruo at 85 °C to give 4_03 kg of iron-dextran powder.
It is understood that the foregoing detailed description and accompanying examples are merely illustrative and are not to be taken as limitations upon the scope of the invention, which 2s is defined solely by the appended claims and their equivalents. Various changes and rnodifications to the disclosed embodiments will be apparent to those skilled in the art. Such cha~lges and modifications, including without limitation those relating to the materials and/or methods of use of the invention, may be made without departing from the spirit and scope thereof.
Claims (12)
1. A process for the preparation of crystalline iron-dextran complex, comprising the steps of:
(a) combining an aqueous solution of dextran in which the dextran has an average molecular of 3,000 to 6,000 with an aqueous solution of an iron(III) salt;
(b) combining the mixture with alkali;
(c) heating and stirring the mixture until the formation of an iron-dextran complex is substantially complete;
(d) precipitating the iron-dextran complex by adding the mixture to a water-miscible organic solvent; and (e) isolating the crystalline iron-dextran complex.
(a) combining an aqueous solution of dextran in which the dextran has an average molecular of 3,000 to 6,000 with an aqueous solution of an iron(III) salt;
(b) combining the mixture with alkali;
(c) heating and stirring the mixture until the formation of an iron-dextran complex is substantially complete;
(d) precipitating the iron-dextran complex by adding the mixture to a water-miscible organic solvent; and (e) isolating the crystalline iron-dextran complex.
2. The process of Claim 1 wherein the water-miscible solvent is methanol.
3. The process of Claim 1 wherein the dextran is selected from the group consisting of dextran, oxidized dextran, and hydrogenated dextran.
4. The crystalline iron-dextran complex prepared according to the process of Claim 3.
5. The process of Claim 3 wherein the dextran is oxidized dextran.
6. The crystalline iron-dextran complex prepared according to the process of Claim 5.
7. The process of Claim 5 wherein the oxidized dextran is prepared by combining sodium hypochlorite and alkali with an aqueous solution of dextran.
8. The crystalline iron-dextran complex prepared according to the process of Claim 7.
9. A process for the crystallization of an iron-dextran complex in readily-filterable form, comprising adding slowly, with stirring, an aqueous iron-dextran solution to a vessel containing a water miscible organic solvent.
10. The process of Claim 9 wherein the aqueous iron dextran solution is added to the water-miscible organic solvent in a ratio of not less than 1:1 by volume.
11. The process of Claim 10 wherein the water-miscible solvent is methanol.
12. The process of Claim 11 wherein the aqueous iron dextran solution is added to the methanol in a ratio of about 1:8 by volume.
13. The crystalline iron-dextran complex prepared according to the process of
12. The process of Claim 11 wherein the aqueous iron dextran solution is added to the methanol in a ratio of about 1:8 by volume.
13. The crystalline iron-dextran complex prepared according to the process of
Claim 12.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US651895P | 1995-11-09 | 1995-11-09 | |
| US60/006,518 | 1995-11-09 | ||
| PCT/US1996/018018 WO1997017377A1 (en) | 1995-11-09 | 1996-11-08 | Process for making crystalline iron dextran |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2236003A1 CA2236003A1 (en) | 1997-05-15 |
| CA2236003C true CA2236003C (en) | 2006-03-14 |
Family
ID=36095766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002236003A Expired - Fee Related CA2236003C (en) | 1995-11-09 | 1996-11-08 | Process for making crystalline iron dextran |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2236003C (en) |
-
1996
- 1996-11-08 CA CA002236003A patent/CA2236003C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2236003A1 (en) | 1997-05-15 |
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