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Improved method for reverse transcription

Info

Publication number
CA2211367A1
CA2211367A1 CA 2211367 CA2211367A CA2211367A1 CA 2211367 A1 CA2211367 A1 CA 2211367A1 CA 2211367 CA2211367 CA 2211367 CA 2211367 A CA2211367 A CA 2211367A CA 2211367 A1 CA2211367 A1 CA 2211367A1
Authority
CA
Grant status
Application
Patent type
Prior art keywords
reverse
mrna
method
performed
transcription
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2211367
Other languages
French (fr)
Other versions
CA2211367C (en )
Inventor
Yoshihide Hayashizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Riken Corp
DNAFORM KK
Original Assignee
Yoshihide Hayashizaki
The Institute Of Physical And Chemical Research
Kabushiki Kaisha Dnaform
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions

Abstract

A method for preparing a cDNA from a mRNA using a reverse transcriptase wherein reverse transcription is performed at a temperature at which the mRNA does not take a secondary structure, for example, at a temperature of 45°C or more. The method is performed, for example, using a heat-labile reverse transcriptase in the presence of a substance exhibiting chaperone function having chaperone function such as saccharides. The method is performed, for example, in the presence of metal ions necessary for activation of the reverse transcriptase and a chelating agent for the metal ions such as a deoxynucleotide triphosphate. The method is capable of reverse transcription over the full length of mRNA template even if the mRNA is a long chain mRNA and, as a result, producing a full length cDNA.
CA 2211367 1996-07-25 1997-07-24 Improved method for reverse transcription Expired - Fee Related CA2211367C (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP196329/1996 1996-07-25
JP19633196A JP3709490B2 (en) 1996-07-25 1996-07-25 Method for improving the thermal stability of the Rna
JP19632996 1996-07-25
JP196331/1996 1996-07-25

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2512701 CA2512701C (en) 1996-07-25 1997-07-24 Improved method for reverse transcription

Publications (2)

Publication Number Publication Date
CA2211367A1 true true CA2211367A1 (en) 1998-01-25
CA2211367C CA2211367C (en) 2008-04-22

Family

ID=26509679

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2211367 Expired - Fee Related CA2211367C (en) 1996-07-25 1997-07-24 Improved method for reverse transcription

Country Status (4)

Country Link
US (3) US6013488A (en)
CA (1) CA2211367C (en)
DE (2) DE69738354D1 (en)
EP (2) EP1243661B1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001523098A (en) 1997-04-22 2001-11-20 ライフ テクノロジーズ,インコーポレイテッド The method for the production of aslv reverse transcriptase comprising a plurality of sub-units
WO1998059075A1 (en) 1997-06-25 1998-12-30 Life Technologies, Inc. Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
DE69937964T2 (en) 1998-11-12 2009-01-02 Invitrogen Corp., Carlsbad Transportreagentien
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
CA2365263A1 (en) * 1999-03-12 2000-09-21 Mcgill University A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor
US6271004B1 (en) * 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
DE10105208A1 (en) * 2001-02-06 2002-08-14 Ihf Inst Fuer Hormon Und Fortp Enzymatic production of cDNA by reverse transcription, in the presence of betaine, provides a high yield of full-length molecules from large RNA templates
ES2188357B1 (en) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Method for amplification by reverse transcription coupled to a reaction of nested or semi-nested PCR in a single step, of a target RNA, and their applications in the identification of enterovirus and poliovirus discrimination between non-polio enteroviruses and.
US20050250100A1 (en) * 2002-06-12 2005-11-10 Yoshihide Hayashizaki Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
US20060084799A1 (en) * 2003-09-24 2006-04-20 Williams Lewis T Human cDNA clones comprising polynucleotides encoding polypeptides and methods of their use
WO2005035569A3 (en) * 2003-10-10 2006-03-02 Five Prime Therapeutics Inc Kiaa0779, splice variants thereof, and methods of their use
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
JP4896006B2 (en) 2004-04-08 2012-03-14 バイオマトリカ, インコーポレイテッド Integration with sample storage and sample management for life sciences
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
JP5187847B2 (en) 2005-05-06 2013-04-24 ジェン−プローブ・インコーポレーテッド Capture methods of nucleic acid target
WO2007011901A3 (en) * 2005-07-15 2007-10-04 Applera Corp Hot start reverse transcription by primer design
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
JP5933544B2 (en) 2010-07-26 2016-06-08 バイオマトリカ, インコーポレーテッド Blood during shipping and storage at ambient temperature dna, compositions for stabilizing rna and proteins and other biological samples
US9458484B2 (en) 2010-10-22 2016-10-04 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
FR2984357B1 (en) 2011-12-16 2016-11-18 Biomerieux Sa Method transcriptional amplification of nucleic acids of different temperatures gathering steps
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
CN106164261A (en) 2014-01-22 2016-11-23 生命技术公司 Novel reverse transcriptases for use in high temperature nucleic acid synthesis

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
EP0035204B2 (en) * 1980-03-05 1991-05-22 Miles Inc. Pasteurized therapeutically active protein compositions
US4409200A (en) * 1980-04-28 1983-10-11 Research Corporation Reverse transcriptase from human milk, method for its purification, and its use in the detection of breast cancer
US5017492A (en) * 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
DE69030386D1 (en) * 1989-12-22 1997-05-07 Hoffmann La Roche transcriptases at high temperature active reverse
DE4124286A1 (en) * 1991-07-22 1993-01-28 Boehringer Mannheim Gmbh A process for the stabilization of proteins in optical tests
FR2690691B1 (en) * 1992-04-29 1999-02-12 Bio Merieux Method for RNA amplification requiring a single step of operation.
JP4372837B2 (en) * 1992-08-04 2009-11-25 ジェン−プローブ・インコーポレイテッドGen−Probe Incorporated Nucleic acid sequence amplification
WO1994012657A1 (en) * 1992-11-20 1994-06-09 Katcher Harold L Direct-phenol pcr, rt and rt-pcr methods
DE4239969A1 (en) * 1992-11-27 1994-06-01 Boehringer Mannheim Gmbh A process for the stabilization of proteins
CA2139623A1 (en) * 1993-05-06 1994-11-24 Baxter Diagnostics Inc. Human papillomavirus detection assay
WO1995014770A1 (en) * 1993-11-25 1995-06-01 Pacific Enzymes (1993) Limited Improved polymerase
DE69531487D1 (en) * 1994-01-31 2003-09-18 Univ California Method for elimination of sequenzierartefakten
DE4411588C1 (en) * 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Buffer, contg. betaine, for RNA- and DNA-polymerase reactions
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

Also Published As

Publication number Publication date Type
EP0821059B8 (en) 2006-03-08 grant
US6372437B2 (en) 2002-04-16 grant
DE69738354D1 (en) 2008-01-17 grant
EP0821059A3 (en) 1998-04-22 application
EP0821059A2 (en) 1998-01-28 application
CA2211367C (en) 2008-04-22 grant
US6013488A (en) 2000-01-11 grant
DE69734913D1 (en) 2006-01-26 grant
EP1243661B1 (en) 2007-12-05 grant
US6221599B1 (en) 2001-04-24 grant
EP0821059B1 (en) 2005-12-21 grant
US20010012617A1 (en) 2001-08-09 application
DE69734913T2 (en) 2006-08-24 grant
EP1243661A1 (en) 2002-09-25 application

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Effective date: 20140724