CA2076430C - Pyrrolidylthiocarbapenem derivative - Google Patents

Abstract

A pyrrolidylthiocarbapenem derivative represented by Formula I is provided:

(I) wherein R1 is hydrogen or lower alkyl; R2, R3 and R4 are hydrogen, lower alkyl which can be substituted or an amino protecting group independently, or R2 and R3 together with a nitrogen atom to which R2 and R3 are bonded form a saturated or unsaturated cyclic group, or R2 and R4, or R3 and R4 together with two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclic group;
each cyclic group can further include at least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be substituted;
X1 is hydrogen or a hydroxy protecting group; X2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali metal or an alkaline-earth metal; and Y2 is hydrogen or an amino protecting group.

Description

20~3~

BACKGROUND OF THE INVENTION

1. Field of -the Invention:
The present invention relates to a new pyrro-lidylthiocarbapenem derivative having a wide range ofan-tibacterial spectrum, an antibacterial agent comprising the carbapenem derivative, a new pyrrolidine derivative which is an intermediate for producing the carbapenem derivative, and a method for producing the pyrrolidylthiocarbapenem derivative and the pyrrolidine derivative.

2. Description of the Prior Art:
Various compounds are known as carbapenems, a ki.nd of a ~-lactam antibiotic. For example, imipenem, meropenem, the mesylate (mesylamino), and -the urea derivatives of a carbapenem as shown below are known.

OH ~ ml ~n~n~ OH Me meropenem rf -s~ ~NHCH=NH ,-.. f \ /~ ~ONH2 o~--N /~ N~ ,NH
COOH COOH

mesylate ure~
OH Me derivative OH Me derivative rl \-S--<'I~'Nt 502Me 'I' rf~ ~NHCONH2 o~--N/~ ,NH o~--N~ <,NH
COOH COOH

All of these compounds have a wide range of antibacterial spectrum, and are effective ayainst both Gram-positive bacteria and Gram-negative bacteria. A

2076~3~

carbapenem derivative having a wider range of antibac-terial spectrum and a stronger antimicrobial activity ha~s been desired.

The pyrrolidylthiocarbapenem derivative of this inven-tion is represented by Formula I:
OXl Rl R~

5~ 2N R3 COOX2 ( I ) wherein R1 is hydrogen or lower alkyl; R2, R3 and R4 are hydrogen, lower alkyl which can be substi-tuted or an amino protecting group independently, or R2and R3 together with a nitrogen atom to which R2 and R3 are bonded form a saturated or unsaturated cyclic group, or R2 and R4, or R3 and R4 together with two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclic group;
each cyclic group can further include at least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be substituted;
Xl is hydrogen or a hydroxy protecting group; x2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali metal or an alkaline-earth metal; and y2 is hydrogen or an amino protecting group.

25In ano-ther aspect of the present invention, the pyrrolidine derivative of the present invention is 2~76~30 represented by Formula Il:

NS02N ~ R3 2 ( I~ ) wherein R2, R3 and R4 are hydrogen, lower alkyl which can be substituted, or an amino protecting group independently, or R2 and R3 toge-ther with a nitrogen atom to which R2 and R3 are bonded form a saturated or unsaturated cyclic group, or R2 and R4, or R3 and R4 together with two nitrogen àtoms and one sulfur atom in -the sufamide group ~orm a saturated or unsaturated cyclic group; each cyclic group can further include at least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be subs-tituted; yl is hydrogen or a mercapto protecting group; and y2 is hydrogen or an amino protecting group.

Alternatively, the presen-t invention provides a method for producing a pyrrolidine derivative repre-sented by Formula II:

~ NSO N<
YlS~ I 2 R3 NY ( II ~

~76~3~

wherein R2, R3 and R4 are hydrogen, lower alkyl which can be subs-tituted, or an amino protecting group independently, or R2 and R3 toge-ther with a ni-trogen atom to which R2 and R3 are bonded form a saturated or unsaturated cyclic group, or R2 and R4, or R3 and R4 together wi-th two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclic ~roup; each cyclic group can further include a-t least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be substituted; yl is hydrogen or a mercapto protec-ting group; and y2 is hydrogen or an amino protecting group; and -the method comprises -the steps of: converting a hydroxy yroup at the 4-position of a 4-hydroxy~yllo-lidine-2-carboxylic acid derivative into a mercapto group; converting a carboxy group at the 2-po~ition into a hydroxymethyl group; converting a hydroxy group in the hydroxymethyl group into an amino group or a sulfamoyl group; and converting the amino group into a sulfamoyl group.

Alternatively the present invention provides a method for producing a pyrrolidylthiocarbapenem derivative comprising the step of: allowing a carbape-nem derivative to react with the pyrrolidine derivative of Formula II to obtain the pyrrolidylthiocarbapenem derivative of Formula I; the carbapenem derivative being represented by Formula III:

2 0 ~

oxl Rl ,1 ~ X3 ( m ) coox2 wherein Rl is hydrogen or lower alkyl; X1 is hydrogen or a hydroxy proteating group; x2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali metal or an alkaline-earth metal; and X3 i5 a leaving group (e.g., reactive ester group of hydroxy, alkyl-sulfinyl arylsulfinyl, alkylsalfonyl, or arylsulfonyl).

Thus, the invention described herein makes possible the advantages of (1) providing a new carbapenem derivative having a strong antimicrobial activity and a wide range of antibacterial spectrum, and a method for producing the carbapenem derivative;
(2) providing a new pyrrolidine derivative which is an intermediate for producing the carbapenem derivative, and a method for producing the pyrrolidine derivative;
and (3) providing an antibacterial agent co-nprising -the carbapenem derivative.

These and other advantages of the present invention will become apparent to those skilled in the art upon reading and understandiny the following de-tailed description.

~OP~3~

DESCRIPTION OF THE PREFERRED EMBOD:tMENTS

Followings are abbreviations used herein:

Ac : acetyl Alz : allyloxycarbonyl Boc : t-bu-toxycarbonyl Et : e-thyl Ft : phthalyl Me : methyl Ms : methanesulfonyl NPrc : protected amino Ph : phenyl PMB : p methoxybenzyl Pmz : p-methoxybenzyloxycarbonyl PNB : p-nitrobenzyl Pnz : p-nitrobenzyloxycarbonyl Tr : trityl Ts : p-toluenesulfonyl A preferred scope of each group herein is as follows:

The number of carbon atoms of "lower alkyl"
is 1 to 6. Examples of such an alkyl group include methyl, ethyl, n-propyl, iso-propyl, n-butyl, t-butyl, pentyl and hexyl. The number of carbon atoms of the lower alkyl is preferably 1 to 4. The most preferred lower alkyl is methyl or ethyl. Examples of a substit-uent of "a substi-tuted lower alkyl" include hydroxy, alkoxy, amino, acylamino, lower alkylamino, carbamoyl, lower alkylcarbamoyl, carbamoyloxy, lower alkylcarba-moyloxy and cyano. The number o~ carbon atoms o~

~7~3~

"arallcyl" is 7 to 15. Examples o.f "an ami.no protecting group" and "a hydroxy protecting yroup" include lower alkoxycarbonyl, lower alkenyloxycarbonyl, halogenoal-koxycarbonyl, aralkyloxycarbonyl, trialkylsilyl and diazo. ~n example of the lower alkoxycarbonyl includes t-butyloxycarbonyl; an example o:E the lower alkenyloxy-carbonyl includes allyloxycarbonyl; examples of the halogenoalkoxycarbonyl include 2-iodoethyloxycarbonyl and 2,2,2-trichloroethyloxycarbonyl; examples o~
the aralkyloxycarbonyl inclucle benzyloxycarbonyl, p-methoxybenzyloxycarbonyl, o-nitrobenzyloxycarbonyl, p-nitrobenzyloxycarbonyl and diphenylmethoxycarbonyl;
examples of the trialkyls:Llyl include trimethylsilyl, triethylsilyl and t-butyldimethylsilyl.
In a definition of a group represented as follows:

~ ~2 ' - N S02 N,~

a saturated or unsaturated cyclic group formed from R2 and R3 together with a nitrogen atom to which R2 and R3 are bonded can be, a saturated or unsatura-ted 3 to 8 membered residue further having one or more of nitro-gen, sulfur and/or oxygen atoms, i.f necessary, and a 5 or 6 membered monocyclic residue including a hetero atom is preferable. The examples include pyrrolidin-1-yl, pyrrol-1-yl, imidazolidin-1-yl, imida-zol-1-yl, pyrazolidin-l-yl, pyrazol-1-yl, piperidino, dihydro- or tetrahydropyridin-l-yl, piperazino, pipera-207~3~1 zin-1-yl wh~ch may have a substituent at the ~-position, morpholino and thiomo:rpholino. These groups may be substituted for one or more, preferably one or two, o~ the following groups: amino, protected amino, carbamoyl, lower alkyl, hydroxy, protected hydroxyl, lower alkoxy, oxo, lower alkylsulfonyl, hydroxy lower alkyl, carbamoyl lower alkyl, lower alkoxycarbonyl and cyano. Moreover, when the cyclic group is imidazoli~
din-l-yl, pyrazolidin-l-yl or piperazin-1-yl, the imino moiety thereof may be protected by a imino protecting group which is known in the art.

In the de~inition of the group IIa, a satu-rated or unsaturated cyclic group formed ~rom R2 and R4, or R3 and R~ can be a saturated or unsaturated 5 to 7 membered residue having 2 to 3 nitrogen atoms and one sulfur atom and if necessary, having an another hetero atom such as an oxygell atom, and 5 to 6 membered mono cyclic residue including a hetero atom is pre~erable.
Such a residue may include, if necessary, a substituent such as lower alkyl, halogen, lower alkoxy, acyloxy, hydroxy, amino, lower alkylamino, acylamino and oxo, and/or an unsaturated bond. ~he examples include 1,1-dioxothiadiazinyl, 1,1-dioxodihydrothiadiazinyl, 1,1,3-trioxodihydrothiadiazinyl, l,l-dioxothiadiazoli-zinyl, 1,1-dioxothiadiazolinyl, and 1,1,3-trioxothia-diazolinyl.

The "carboxy protec-ting group" is selected ~rom those used in the art and serve the ~unction of blocking the carboxyl group while reactions are carried out at other sites of the molecule. Such group gener-ally contains less than about 19 carbon atoms and bind .. . .

2 ~ 3 ~

to a carboxyl ~roup reversibly without affecting the other parts of the molecule. Typical examples include following groups: optiorlally substituted C1 - C8 alkyl, for example, rnethyl, methoxymethyl, ethyl, ethoxymethyl, iodomethyl, propyl, isopropyl, bu~yl, isobutyl, ethoxyethyl, methylthioethyl, me-thanesulfony-lethyl, trichloroethyl, t-butyl, and the like; option-ally substituted C3 - C8 alkenyl, for example, propenyl, allyl, isoprenyl, hexenyl, phenylpropenyl, dimethylhexenyl, and the like; optionally substituted C7 - C19 aralkyl, for example, benzyl, methylbenzyl, dimethylbenzyl, methoxybenzyl, e-thoxyben~yl, nitro-benzyl, aminobenzyl, diphen~lmethyl, phenylethyl, trityl, di-t~butylhydroxybenzyl, phthalidyl, phenacyl, and the like; optionally substituted C6 - ~12 aryl, for example, phenyl, toluyl dii.sopropylphenyl, xylyl, trichlorphenyl, pentachlorophenyl, indanyl, and the like; optionally subs-tituted C~ - C12 amino which is, e.g., an ester with acetone oxime, acetophenone oxime, acetoaldoxime, N-hydroxysuccineimide, N-hydroxyphthali-mide, or the like; optionally substituted C3 - C12 hydrocarbonated silyl, for example, trimethylsilyl, dimethylmethoxysilyl, t-butyldimethylsilyl, and the like; optionally substituted C3 - C12 hydrocarbonated stannyl, for example,-trimethylstannyl, and the like.
Another carboxy protecting group is a pharmaceutically active ester forming group. Examples of such a group include following groups: l-(oxgen-substituted)-C~ to C15 alkyl groups, for example, a straight, branched, ringed, or partially ringed alkanoyloxyalkyl, such as ace-toxymethyl, acetoxyethyl, propionyloxymethyl, pivaloyloxymethyl, pivaloyoxyethyl, cyclohexaneacetoxy-ethyl, cyclohexanecarbonyloxycyclohexylmethyl, and the 207S~3~

like; C3 - C15 alkoxycarbonyloxyalkyl such as ethoxyca~
bonyloxyethly, and the like; C2 - C8 alkoxyalkyl, such as methoxymethyl, me-thoxyethyl, and -the like; C~ - C8 2-oxacycloalkyls, such as tetraphdropyranyl, tetrahy-drofuranyl, and the like; substituted C8 - C12 aral-kyls, for example, phenacyl, phthalidyl., and the like;
C6 -C12 aryl, ~or example, phenyl, xylyl, indanyl, and the like; C2 - C12 alkenyl, for example, allyl, isopre-nyl, 2-oxo-1,3-dioxolyl-4-y].-methyl, and the like.
Among the above, a protecting group used to block the carboxyl group during reactions is usually removed at the final step of the reaction, and therefore its stxu~,ture is not essential. Thus, as one of skilled in the art can easily appreciate, the carboxy protecting group can be selected from various equivalent groups including amides, acid anhydrides ~ormed with carbonic acid or carboxylic acids, and the like as long as an aimed carboxyl group is protected properly.

An example of -the lower alkyl includes t-butyl; examples o~ the lower alkenyl include allyl, isopentenyl and 2-butenyl; examples of the halogeno lower alkyl include 2-iodoethyl and 2,2,2-trlchloroeth-yl; examples of the lower alkoxymethyl include methoxy-methyl, ethoxymethyl and isobutoxymethyl; examples of the lower aliphatic acyloxymethyl include acetoxymeth-yl, proplonyloxymethyl, butyryloxymethyl and pi~aloy-loxymethyl; examples of the 1-lower alkoxycarbonylox-yethyl include l-methoxycarbonyloxyethyl and l-ethoxy-carbonyloxyethyl; and examples of the aralkyl include benzyl, p-methoxybenzyl, o-nitrobenzyl, p-nitrobenzyl and diphenylmethyl. Examples of "an alkali metal"
include lithium, sodium and potassium, and sodium or ~7~3~

potassium is preferred. Examples of "an alkaline-earth metal" include magnesium and calcium.

AS " a mercapto protecting group", a conven-tional one, e.g., acyl and aryl substituted lower alkylsuch as benzyl, phenethyl, trityl and benzhydryl are included. As "a reactive estex group of hydroxy", a conventional one, e.g., a residue such as substituted or unsubsti-tuted arylsulfonyloxy, lower alkanesulfony-loxy, halogeno lower alkanesulfonyloxy, dialkylphospho-nyloxy, diarylphosphoryloxy and halogeno are included.
Examples of the arylsulfonyloxy include benzenesulfon-nyloxy, p-toluenesulfonyloxy, p-nitrobenzenesulfonyloxy and p-bromobenzenesulfonyloxy; examples of the lower alkanesulfonyloxy include methanesulfonyloxy and eth-anesulfonyloxy; an example of the halogeno lower al-kanesulfonyloxy includes trifluoromethanesulfonyloxy, an example of the dialkylphospheoryloxy includes dieth-ylphosphoryloxy; an example of the diarylphosphoryloxy includes diphenylphosphoryloxy; and examples of the halogeno include chloro, bromo and iodo.

An example of "an alkylsulfinyl group" in-cludes methylsulfinyl, and an example of "an arylsulfi-nyl group" includes phenylsulfinyl.

The pyrrolidylthiocarbapenem derivative ofthe present invention is represented by the following Formula I:

~6~3~

OXI ~ I R4 R2 COOX2 ( I ) wherein Rl is hydrogen or lower alkyl; R2, R3 and R4 are hydrogen, lower alkyl which can be substi-tuted or an amino protecting group independently, and pre~erably R4 is hydrogen, or R2 and R3 together with a ni-trogen atom to which R2 and R3 are bonded ~orm a saturated or unsaturated cyclic group, or R2 and R4, or R3 and R4 together with two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclia group and each cyclic group can further include at least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be substituted; X1 is hydroyen or a hydroxy protecting group; x2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali rnetal or an alkaline-earth metal; and y2 is hydrogen or an amino protecting group.

When the above pyrrolidylthiocarbapenem derivative I has a free -OH, -COOH, amino group, imino group, or substituted amino group, the pyrrolidylthio-carbapenem also includes pharmaceutically acceptable salts thereof. The same is the case with an intermedi-ate compound for synthesizing the pyrrolidylthiocar-bapenem derivative such as the pyrrolidine derivative 207~3~) - 13 ~

represented by Formula II. Examples of the pharmaceu-tically acceptable salts include a salt with a base, a salt with an acid, a salt with a basic or acidic amino acid and an intermolecular or intramolecular quarter-nary salt. Examples of the salt wi-th a base include alkali metal salts such as sodium salt and potassium salt; alkaline-earth metal salt:s such as calcium salt and magnesium salt; ammonium salt; and organic amine salts such as triethylamine sal~, pyridine salt, pico-line salt, ethanolamine salt, triethanolamine salt,dicyclohexylamine salt, N,N'-dibenzylethylenediamine salt and dibenzylamine salt. Examples of the salt with an acid include inorganic acid addition salts such as hydrochloride, hydrobromide, sulfuric acid salt and phosphoric acid salt; and organic acid addition salts such as formic acid salt, acetic acid salt, trifluoroa-cetic acid salt, maleic acid salt, tartaric acid salt, methanesulfonic acid salt, benzenesulfonic acid salt and -toluenesulfonic acid salt. Examples o~ the salt with an amino acid include a salt with arginine, aspar-tic acid or glutamic acid.

The pyrrolidylthiocarbapenem derivative (I) of the present invention can be produced in the steps of: by using, for example, 4-hydroxypyrrolidine-2~
carboxylic acid or the derivative thereof as a starting material, obtaining a pyrrolidine derivative II repre~
sented by the following formula:

yls~NS~2N < R3 2 ( I I ~

2a7~3~

wherein R2, R3 and ~ are hydrogen, lower alkyl which can be subst.ituted, or an amino protectiny group independently, and R4 is preferably hydrogen, or R2 and R3 toge-ther wi.th a nitro(~en atom to which R2 and R3 are bonded form a saturated or unsaturated cyclic group, or R2 and R~, or R3 and R~ together with two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclic group;
each cyclic group can further include one atom selected from the group consisting of oxygen, sulfur and nitro-gen, and each cyclic group can be substi-tuted; yl is hydrogen or a mercapto pro-tecting group; and y2 is hydrogen or an amino protecting group; and allowing the obtained pyrrolidine derivative II to react with a carbapenem derivative represented by the following Formula III:

oxl Rl COOx2 (III) wherein R1 is hydrogen or lower alkyl; Xl is hydro~en or a hydroxy protecting group; x2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali metal or an alkaline-earth metal; and X3 is leaving group (e.g., a reactive ester of hydroxy, alkylsulfi-nyl, arylsul:Einyl, alkylsulfonyl, or arylsulfonyl).

The present invention also includes a pyrro-lidine derivative represented by the following Formula II:

2~7~3~

yls~NS~2N < R3 2 ( II ) wherein R2, R3 and R4 are hydrogen, substitu-ent lower alkyl which can be substituted, or an amino protecting group independently, and R4 is preferably hydrogen, or R2 and R3 together with a nitrogen atom to which R2 and R3 are bonded form a saturated or unsatu-rated cyclic group, or R2 and ~4, or R3 and R4 together with two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclic group; each cyclic group can further include at least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be substituted; yl is hydrogen or a mercapto protecting group; and y2 is hydrogen or an amino protecting group.

The pyrrolidine derivative II is prepared according to the steps of converting a hydroxy group at the 4-position of a 4-hydroxypyrrolidine-2-carboxylic acid derivative into a mercapto group; converting a carboxy group at the 2-position into a hydroxymethyl group; directly sulfamidating a hydroxy group in the hydroxymethyl group or sulfamoylating it after convert-ing it into an amino group; and removing the protecting group Y~ if necessary. The order of these steps can be properly changed.

3 ~

Synthesis of pyrrolldine derivatlve II
Pyrrolldlne derivative II is synthesized, for example, in the following process, but is not limited to.

Route l HO' y~
~COOR~ N ~OR; N H
' 2 y2 y2 (IV) (~ ) (~) yl5~ NS~2 N < R3 y2 y_ In the above scheme, R2, R3 and R4 are the same as defined for Formula I, and R5 is a group for forming an ester together with a carboxy group such as lower alkyl. yl and y2 are the same as defined for Formulas I and II, but denote a mercapto protecting group and an amino protecting group, respectively, at the intermediate of the reaction route.

In this process , for example, 4-hydroxypyr-rolidine-2-carboxylic acid derivative IV is first provided. A mesyl group or the like is introduced to the hydroxy group at the 4-position of compound IV, and then a protected mercapto group such as a tritylthio 3 ~

group is introduced to the ~-position. In this way, a compound V is obtained. Then, a compound VI is ob-tained by reducing a carboxylate group at the 2 position. An azide group i5 introduced to the com pound VI, and the azide group i.5 converted to an amino group, or phthalimide is reacted with the compound VI, and the formed phthalyl group is removed from the com-pound VI, thereby introducing an amino group at a position o~ the hydroxy group o~ the compound VI.
Thus, a compound VII is obtained. A sul~amoyl group is then introduced to the compound VII to obtain a com-pound II.

Furthermore, the process of Route 1 can be variously modified. For example, after introducing a protected mercapto group to the 4-position of the compound IV, a carboxylate group is reduced and then a sulfamide group is introduced to obtain the compound II. Alternatively, after reducing the com pound IV, a protected mercapto group and a sulfamide group are successively introduced to obtain the com-pound II.

Route 2 HQ.............X40.............. X40.
R5 1 ~ H ~ N~c y2 (IV) y2 (~) y2 (IX) NPrc ~N~NH2~ NS02N~R2 y2 (X) y2 (~) y2 (II-1) '. ~' '': - ' ' - ' . " ' ~ ' .

6~3~

In the above scheme, R2, R3 and R4 are the same as defined in Formula I, and R5 is a group for forming ester together with a carboxy group such as lower alkyl. yl and y2 are the same as defined in F~ormulas I and II, but denote a rnercapto protecting group and an amino protecting group, respectively, at the intermediate of the reaction route. X4 is a hy-droxy protecting group.

In thls process, for example, a mesyl group or the like (represented by X4) is first introduced to the ~-position of the 4-hydroxypyrrolidine-2-carboxylic acid derivative IV, then a carboxylate group is reduced to a hydroxymethyl group as is in Route l to obtain a compound VIII. Then, a protected amino group such as a phthalimide group is :Lntroduced to a position of a hydroxy group in the hydroxymethyl ~roup. Thus a com-pound IX is obtained. In introducing the protected amino group, it is effec-tive to introduce a leaving group to the hydroxy group of the compound VIII to increase the reactivity. Next, a mercapto group pro-tected by thioacetate and the like (represented by YlS) is introduced to the 4-position (see a compound X), and removing the protection to obtain a compound XI. By introducing a sulfamoyl group to the compound XI, a compound II-l (a compound II wherein the -SY' at the 2-position of the pyrrolidine ring is SH) is obtained.

Furthermore, the process of Route 2 can be variously modiEied. For example, by introducing a pro-tected mercap-to group to the 4-position of the com-pound VIII, further introducing a sulfamoyl group and removing the protection, the compound II-l is obtained.

2 a ~ 3 0 Route 3 HO ~ ~0- ~ HO ~ ~

y2 y2 y2 tIV-!) OE~:¢) (~) .

HO R4 R? HS R~ R2 ~ ~ ~rSO2N~ 3 ~ so2N ~ ~3 y2 c ~ 3 y2 L~-l~

In the above scheme, R2, R3 and R4 are the same as defined in Formula I. y2 is -the same as de-fined in Formula I but denotes a protecting amino group at the intermediate of the reaction route.
s In this method, chloroformate or the like is first allowed to react with 4-hydroxypyrrolidine-2-carboxylic acid IV-l having protected nitrogen in the pyrrolidine ring. A carboxy group at the 2-posi-tion is then converted into a hydroxymethyl group by reduction.
Next, after converting a hydroxy group in the hydroxy-methyl group into a reactive ester and introducing a protected amino group, a compound XIII is obtained by removing the protection. A sulfamoyl group is intro-duced to the compound XIII resul-ting in a compound XIV, then, a protected mercapto group is introduced to a position of -the hydroxy group at the 4-pos tion. A
compound II-l is obtained by removing the protection of the mercapto group.

2~7S~

Synthesis of a pyrrolidylthiocarbapenem derivative The protection of the ~-position o-f the pyrrolidine derivative is removed to obtain an SH
compound, if necessary, then, the pyrrolidine deriva~
tive is allowed to react with a carbapenem derivative represented by the following ~ormula III to give a pyrrolidylthiocarbapenem derivative I of the present invention:
oxl Rl Jor ~ X3 ( ~ ) coox2 wherein R1 is hydrogen or lower alkyl; X1 is hydrogen or a hydroxy protecting group; x2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali metal or an alkaline-earth metal; X is a leaving group (e.g., reactive ester group of hydroxy, alkylsulfinyl, arylsulfinyl, alkylsulfonyl, or arylsufonyl).
The protection is removed from the compound I
if necessary to give a compound having free carboxy, hydroxy and/or amino.

An antibacterial agent comprising the pyrrolidylthio-carbapenem derivative A pharmacentical composition comprising the pyrrolidylthiocarbapenem derivative (including pharma-centically acceptable salts thereof) of the present invention is administered as an an-tibacterial agent.
An administration method is in oral administration or parenteral administration; as injection (a formulation 7,(~7~3~

in an ampoule or vial, a liquid, a suspension or the like for an intravenous injection, an intramuscular injection, a drip infusion, or subcutaneous injection), an external or local administration agent (an ear drop, a nasal drop, an ophthalmic solution, an ointment, an emulsion, a spray, a suppository and the like), and an oral preparation. Preferably, it the composition is administered by injection, through skin or mucosa.
The pharmaceutical composition includes at least 0.01~
by weight of the pyrrolidylthiocarbapenem derivative and further includes an appropriate excipient, auxil-iary agen-t, stabilizer, wetting agent, emulsifier, and other additives depending upon the administration method. These addi-tives must to be pharmaceutically and pharrnacologically acceptable materials which do not inhibit the effect of the pyrrolidylthiocarbapenem derivative and which show no adverse effects on pa-tients. For example, lactose, stearic acid, magnesium stearate, clay, sucrose, cornstarch, -talc, gelatin, agar, pectin, peanu-t oil, olive oil, cacao butter, ethylene glycol, tartaric acid, citric acid and fumaric acid can be contained in the oral preparation. For pa-ren-teral administration, a solvent (e.g., alcohol, a buffer, methyl oleate, water or the like), a buffer solution, a dispersing agent, a dissolving auxiliary agent, a stabilizer (e.g, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, sorbic acid or the like), an absorbefacient (mono- or dioctanoate of glycerin), an antioxidant, a perfume, an analgetic, a dispersing agent, an adverse effect inhibitor, an ac-tion potentia-tor (an agent for regulating absorption and elimina~
tion, an inhibitor for enzyme decomposition, a ~-lacta-mase inhibitor, and other kinds of antimicrobial 207~30 agents) and the li.ke can be contained in -the formula~
tion.

A dose of the pyrrolidylthiocarbapanern deriv-ative of the present invention depends upon the age of a patient, the type and the state of the disease and the kind of compounds to be used. Generally, daily dose ranges from 1 mg/patient to about ~000 mg/patient, but more can be administered if necessary. For exam-ple, a dose of 1 mg (the external application) isadministered ~ times a day, and a dose of 1000 mg (intravenous injection) is administered 2 to 4 times a day to -treat an infection.

Characteristics of the pyrrolidylthiocarbapenem deriva-tive The characteristics of the pyrrolidylthiocar-bapenem derivative of the present invention as an antibacterial agen-t will now be described as compared with same known compounds.

(1) Antimicrobial activity:
A minimum inhibitory grouwth inhibitory con-centration and an effect for preventing bacterial 25 ' infection of the pyrrolidylthiocarbapenem derivative of the present invention are compared with those of meropenem (Japanese Laid Open Patent Publication No. 60-233076) and imipenem (Japanese Laid Open Patent Publication No. 55-9090), respectively to find that the derivative of the present invention is superior -to meropenem against Gram positive bacteria and superior to imipenem against Gram negative bacteria. The deriv-ative of the present invention has an antibacterial 2 ~ 3 rJ

potency against Pseudomonas aeruginosa, a kind of a Gram negative bac-teria, equal to or twice as that of imipenem, meropenem and the mesylamino derivative of a carbapenem (Japanese Laid Ope,n Patent Publication No. 63-179876). When compared with the urea derivative of a carbapenem (Japanese Laid Open Paten-t Publication No. 62-155279), the derivative has equal to or twice the antibacterial potency against Gram positive bacte-ria, twice the potency against the Gram negatlve bacte-ria and twice to eight times the potency against Pseu-domonas aeruginosa.

(2) Rabbit nephrotoxicity test:
An administration of the derivative of the present invention of 250 mg per 1 kg of the body weight of a rabbit reveals no toxicity. The same result is obtained by an administration o~ meropenem. When 150 mg/kg of imipenem is administered, medium renal toxicity is revealed. Sugar and protein are found in urine and a white microgranular change in the kidney is found.

(3) Rate of decomposition by mouse renal dehydropepti-dase 1:
The enzymatic decomposition rate of the pyrrolidvlthiocarbapenem derivative of the present invention by the action of renal dehydropectidase I is 76% of that of imipenem, 40% of that of meropenem to show higher stability.

(4) Solubility in water:
The solubility in water of ~he derivative of the present invention is ~0% or more in a form of free 3 ~

-- 2~ -acid, enabling an intravenous injection. In contrast, the solubili-ty of imipenem and meropenem is abou-t 2 and it cannot be administered except for a drip infu sion.

(5) Pharmacokinetics in vivo:
When the derivative of the present invention is intravenously injected to a cynomolgus (10 mg/kg), the half-life is 1.1 hours, a recovery from urine is 62.2%, and an integrated value of a concentration in blood is 24.9 ~g-hr/ml. The half-life is l.A4 times, the recovery ~rom urine is 1.36 times and the integrat-ed value of a concentration in blood (Area under the curve: AUC) is 1.44 times as much as those of merope-nem. The half-life is 1.87 times, the recovery from urine is 1.93 times, and AUC is 1.87 times as much as those of imipenem.

When the derivative is intravenously injected into a mouse (20 mg/kg), the recovery from urine is 36.3~, and the integrated value of a concentration in blood is 12.1 ~g-hr/ml. The recovery from urine is 2.18 times and AUC is 2.32 times as much as those of meropenem. The recovery from urine is 1.15 times and AUC is 1.37 times as much as those of imipenem. The recovery from urine is 1.48 -times as much as that of mesylate derivative of meropenem.

In this way, the present invention provides a new pyrrolidylthiocarbapenem derivative having a widé
range of antibacterial spectrum and a strong antimicro-bial activity against both Gram positive bacteria and Gram negative bacteria, an antibacterial agent (compo-~7~3~

sition) comprising the carbapenem derivative, and a method for preparing the carbapenem derivative. Fur-thermore, a new pyrrolidin derivative as an intermedi-ate ~or preparing the carbapenem derivative and a method for preparing the same are provided.

A minimu~l bacterial growth inhibitory concen-tration and an effect ~or preverrting bacterial infec-tion of the pyrrolidylthiocarbapenem derivative of the present invention are compared with those of meropenern and imipenem, respectively to ~ind that the derivative of the present invention is superior to meropenem against Gram positive bacteria and superior to imipenem against Gram negative bacteria. The derivative of the present invention has an antibacterial potency against Pseudomonas aerugino~a, a kind of a Gram negative bacterium, equal to or twice of that o-f imipenem, meropenem and the mesylamino derivative of a carbape-nem. When compared with the urea derivative of a carbapenem, the derivative has an equal or twice the antibacterial potency against Gram positive bacteria, twice the potency against the Gram nega-tive bacteria and twice to eight times the potency against Pseudomo-nas aeruginosa. The pyrrolidylthiocarbapenem deriva-tive is less toxic to an organism than the conventionalcarbapenem derivatives. Since the derivative decom-poses slowly in a body, the antimicrobial effect there-of lasts for a longer period of time. Moreover, since the derivative has a higher solubili-ty in water than the conventional carbapenem derivatives, it can be applicable for injection.

~7~

Following Examples are given to show the present invention, but not to limit the scope therof.

Preparative Example 1 of a pyrro:Lidine derivative H~" Ms~. TrS~___ I~ ~COOMe 1 ~ Me 2 ~N ~ Me 3 Pmz Pmz Pmz {)H 4 S~NJ~)MS ~ S~N3 Pmz Pmz Pmz 17 l6 TrS~ TrS~
Ft - 8--~ ~NH2 Pmz Pmz 1 9 ~

~,NHS02NMe2 ~2~2Npm2 Pmz p~ 13 Pmz o l 12 l 14 N1 ~ 2NMe2 ~ ~ ~ 2NHP~ ~ ,NHS02NPn~
Pmz Pmz Pmz ~07~3~

Step 1. Preparation of an O mesyl compound To a solution of (2S,4R)-1-p-methoxybenzy-loxycarbonyl~4-hydroxypyrrolidine-2-carboxylic acid methyl ester (227.2 g: 0.735 mole) in dichloromethane (1.3 liter~ stirring at -30~C, triethylamine (112.5 ml:
1.1 eq.) and methanesulfonyl chloride (56.8 ml: 1 eq.) are added. The mixture is stirred at the same tempera-ture for 15 minutes. The reaction mixture is succes-sively washed with dilute hydrochloric acid and water, dried over magnesium sulfate, and concentrated in vacuo to give (2S,4R)-l-p-methoxybenzyloxy-carbonyl-4-meth-anesulfonyloxypyrrolidine-2-carboxylic acid methyl ester (280.1 g). Yield: 98%.
NMR ~ (CDCl3) ppm: 3.02, 3.04(2 x s, 3~), 3.56, 3.78(2 x s, 3H), 3.81(s, 3H), 4.98, 5.08(ABq, J=12Hz, lH), 5.04, 5.12(ABq, J~12H~, lH).
IR ~ (CHCl3) cm~l: 1755, 1709, 1620.

Step 2. Preparation of a tritylthio compound To a solution of triphenylmethylmercaptan (107.02 g: 1.5 eq.) in dimethylformamide (350 ml), an oil suspension containing 60% sodium hydride (13.42 g:
1.3 eq.) is added with stirring at 0~C. The mixture is stirred at room temperature for 1 hour. The reaction mixture is mixed with a solution of (2S,4R)-l-p-met-hoxybenzyloxycarbonyl-4-methanesulfonyloxypyrrolidine-2-carboxylic acid methyl ester (100 g: 0.258 mole) in dimethylformamide(70 ml) with stirring at 0~C. The mixture is stirred at 60~C for 30 minutes. The reaction mixture is poured into cold dilute hydro-chloric acid, and extracted with ethyl acetate.
The extract is successively washed with water and brine, dried, and concen-trated in vacuo. The residue 2~7~3~

is purified by silica gel co]umn chroma~ography (toluene : ethyl acetate = 5 : 1) to give (2S,4S)-1-p-methoxybenzyloxycarbonyl-4-tritylthiopyrrolidine-2-carboxylic acid methyl ester (127.1 g). Yield: 87%.
NMR ~ (CDC13) ppm: 3 50, 3.71(2 x s, 3H), 3.78, 3.84(2 x s, 3H), 4.87, 5.13(ABq, J=12H~, lH), 4.89, 5.13(ABq, J=12Hz, lH).
IR ~ (CHC13) cm~1: 1750, 1700, 1618.

Step 3. Preparation of a me-thylol compound To a solution of (2S,4S)-1-p-methoxybenzy-loxycarbonyl-4-tritylthiopyrrolidine-2-carboxylic acid methyl ester tl27.1 g: 0.224 mole) in tetrahydrofuran (1 liter), lithium borohyride (4.88 g: 1 eq.) is added with stirring at room temperature. The mixture is stirred at 60~C for 30 minutes. The reaction mixture is allowed to cool to room temperature and water (100 ml) is added in small portions with stirring. The formed precipitate is removed by filtration and the filtrate is concentrated in vacuo. The residue is dis-solved in dichloromethane, dried over magnesium sul-fate, and concentrated under reduced pressure. The residue is washed with ether to give (2S,4S)-1-p-methoxybenzyloxycarbonyl-4-tritylthiopyrrolidine-2-methanol as white crystals (82.3 g). Yield: 68%.
NMR ~ (CDC13) ppm: 3.84(s, 3H), 4.93, 4.99(ABq, J=12Hz, 2H).
IR ~ (CHC13) cm~1: 3400, 1668, 1610.

Step 4. Preparation of a mesyl compound A solution of (2S,4S)-1-p-methoxybenzyloxy-carbonyl-4-tritylthiopyrrolidine-2-methanol (22.33 g:
41.37 mmole) is diluted with dichloromethane (300 ml) ~0'~3~

and -the mixture is cooled to -30~C. To this rnixture, triethylamine (6.92 ml: 1.2 eq.) and methanesulfonyl chloride (3.52 ml: 1.1 eq.) are added, and the mixture is stirred for 20 minutes. The reaction mixture is successively washed with dilute hydrochloric acid and water, dried over magnesium sulfate, and filtered. The filtrate is concentrated in vacuo to give crude (2S,4S)-1-p-methoxybenzyloxycarbonyl-4-tritylthiopyrro-lidine-2-methanol me-thanesulfonate (27.81 y:
45.02 mmole). Yield: 100%.
NMR ~ (CDC13) ppm: 2.89(s, 3H), 3.81, 3.83(2 x s, 3H), 4.85 to 5.07(m, 2H).
IR ~ (CHC13) cm~1: 1725, 1690, 1610.

Step 5. Preparation of an azide compound To a solution of (ZS,4S)-1-p-methoxybenzy-loxycarbonyl-A-trityl-thiopyrrolidine-2-methanol meth-anesulfonate (27.81 g) in dimethylformamide (120 ml), a solution (12 ml) of sodium azide (3.50 g: 53.8 mmole) 2,0 in water is added. The mixture is stirred at 80~C for 8 hours. The reaction mix-ture is poured into ice water and extracted with ethyl acetate. The ex-tract is successively washed with water and brine and concen-trated. The residue is purified by silica gel column chromatography to give (2S,4S)-1-p-methoxybenzyloxycar-bonyl-2-azidomethyl-4-tritylthiopyrrolidine (17.27 g:
30.64 mmole). Total yield o~- Steps 4 and 5: 74%.
NMR ~ (CDC13) ppm: 3.84(s, 3H), 4.82 to 5.15(m, 2H).
IR ~ (CHC13) cm~1: 2105, 1685.
Step 6. Preparation of an amino compound A solution of (2S,4S)-1-p-methoxybenzyloxy-carbonyl-2-azidome-thyl-4-tritylthiopyrrolidine 207~3f) (17.27 y: 30.64 mmole) in a mixture of ethyl acetate (150 ml), methanol (200 ml), and acetic acid (2.63 ml:
46 mmole) is subjected to conventional hydrogenation over 5% palladium on carbon (5 g). After -the reaction, the catalyst is filtered off and -the filtrate is con-centrated in vacuo to give (2S,4S)-1-p-methoxybenzy-loxycarbonyl-2-aminomethyl-4-tri-tylthiopyrrolidine acetate (17.33 g) as a residue. The residue is dis-solved in dichloromethane, washed with aqueous sodium hydrogen carbonate, and concentrated to give (2S,4S)~2-aminomethyl-1-p-methoxybenzyloxycarbonyl-4-tritylthiopyrrolidine (16.82 y).

Step 7. Preparation of a ph-thalimido compound Cru~e (2S,4S)-1-p-methoxybenzyloxycarbonyl-4-tritylthiopyrrolidine-2-methanol me-thanesul~onate (115.4 g) produced from (2S,4S)-l-p-methoxybenzyloxy-carbonyl-4-tritylthiopyrrolidine-2-methanol (96.24 g:
178 mmole) in the same mamler as in the above-mentioned Step 4 is dissolved in dimethylformamide (1 liter).
After adding potassium phthalimide (65.94 g: 2 eq.), the mixture is stirred at 100~C for 1 hour. The reac-tion mixture is poured into ice water and extracted with e~hyl acetate. The extract is successively washed with water and brine, and concentrated. The residue is puri~ied by silica gel column chromatography (toluene : ethyl acetate) to give (2S,4S)-l-p-met-hoxybenzylo~ycarbonyl-2-phthalimidomethyl-4-tritylthio-pyrrolidine (99.4 g). Yield: 83.5~.
NMR ~ (CDCl3) ppm: 3.78, 3.84(2 x s, 3H), 4.65 to 5.00(m, 2H).
IR ~ (C~Cl3) cm 1 1770, 1712, 1693, 1611.

2~7~

~ 31 -Step 8. Removal of a phthalyl group To a solution of (2S,4S)-1-p-methoxybenzy-loxycarbonyl-2~phthalimidomethyl~4~tri-tylthiopyrroli-dine (752 mg: 1.124 mmole) in a mixture of dichloro-methane (3 ml) and methanol (12 ml), hydrazine hydrate (109 ,ul: 2 eq.) is added. The mixture is heated ~or 5 hours. The reaction mixture is concen-tratecl in vacuo. The residue is dissolved in dichloromethane (5 ml) and -the solid is filtered off. The filtrate is washed with water and concentrated in vacuo.
The residue i5 recrystallized from a mixture of dichloromethane and methanol to give (2S,4S)-1-p-methoxybenzyloxycarbonyl-2-aminomethyl-4-tritylthiopyr-rolidine (471 mg). Yield: 78%. mp. 165 to 167~C.
NMR ~ ( CDCl3:CD30D=2:1) ppm: 3.46(s, 3H), ~.96, 4.89(ABq, J=12Hz, 2H).
IR ~ (CHC13) cm~1: 1683, 1610.

Step 9. Preparation of a dimethylsulfamoyl compound A solution of (2S,4S)-1-p-methoxybenzyloxy-carbonyl-2-aminomethyl-4-tritylthiopyrrolidine (12.44 g: 23.13 mmole) in dichloromethane(70 ml) is cooled to -78~C. After adding triethylamine (4.21 ml:
1.3 eq.) and dimethylaminosul~onyl chloride (2.73 ml:
1.1 eq.), ths mixture is warmed to room temperature over about 1 hour. The reaction mixture is successive-ly washed with dilute hydrochloric acid and brine, and concentrated to give crude (2S,4S)-1-p-methoxybenzyloxycarbonyl-2-N,N-dimethylsulfamoylamino-methyl-4-tritylthiopyrrolidine (15.02 g). Yield: 100%.

3 ~3 Step 10. Preparation of a mercap~o compound by depro-tection To a solution of (2S,4S)-1-p-methoxybenzy-loxycarbonyl-2-N,N-dime-thylsuLfamoylaminomethyl-4-tritylthiopyrrolidine (3.55 g: 5.5 mmole) in a mixture of dichloromethane (70 ml) and methanol (35 ml), a solution of pyridine (0.66 ml: 1.5 eq.) and silver nitrate (1.40 g: 1.5 e~.) in water (3.5 ml) is added under ice cooling. The mixture is stirred for 10 minutes. The reaction mixture is poured into water and extraated with dichloromethane. The extract is dried over magnesium sul~ate, bubbled with hydrogen sulfide, and filtered to remove solid. The ~iltrate is concentrated in vacuo and the residue is purified by silica gel column chromatography (toluene : ethyl acetate) to give (2S,4S)-1-p-methoxybenzyloxycar-bonyl-2-N,N-dimethylsulfamoylaminomethyl-4-mercaptopyr-rolidine (1.93 g). Yield: 87.0%.
NMR ~ (CDC13) ppm: 2.77(s, 6H), 3.81(s, 3H), 5.00 to 1.12(m, 2H).
IR ~ (CHCl3) cm 1 3380, 1690, 1610.

Step 11. Preparation of a sulfamoyl compound To a solution of chlorosulfonyl isocyanate (3.95 ml: 45.4 mmole) in dichloromethane (70 ml), p-methoxybenzyl alcohol (5.66 ml: 45.4 mmole) is added at -50~C. The mixture is stirred at -50~C for 15 minutes. The resulting solution of p-methoxybenzy-loxycarbonylsulfamoyl chloride is added to a solution of (2S,4S)-1-p-methoxybenzyloxycarbonyl-2-aminomethyl-4-tritylthiopyrrolidine (obtained in the above Steps 6 or 8) (12.21 g: 22.7 mmole) and t-iethylamine (6.38 ml:
45.6 mmole) in dichloromethane (300 ml) at -78~C, and ,.~

~7~3~

the mixture i9 s-tirred for 10 minutes, successively washed with dilute hydrochloric acid and bri.ne, and concentra-ted in vacuo. The residue is purified by silica gel eolumn chromatography to give (2S,4S)-1-p-methox~7benzyloxycarbonyl-2-p-methoxybenzyloxycarbonyl-sulfamoylaminomethyl-4-tritylthiopyrrolidine (16.31 g).
Yield: 91.6~.
NMR ~ (CDCl3) ppm: 3.78(s, 3H), 3.81, 3.83(2 x s, 3H), 4.98, 4.89(ABq, J=12Hz, 2H), 5.09, 5.03(ABq, J=12Hz, 2H).
IR ~ (CHC13) cm~1: 3390, 1740, 1685.

Step 12. Preparation of a mercap-to eompound by depro-teeti.on To a solution of (2S,4S) 1-p-methoxybenzy-loxycaronyl-2-p-methoxybenzyloxycarbonylsulfamoylamino-methyl-4-tritylthiopyrrolidine (2.35 g: 3.13 mmole) in a mixture of dichloromethane (60 ml) and methanol (30 ml), a solution of pyridine (0.38 ml: 4.75 mmole:
~1.5 eq.) and silver nitrate (0.80 g: I.5 eq.) in water (2 ml) is added under ice cooling. The mixture is stirred for 10 m~nutes. The reaction mixture is poured into water and extracted with dichloromethane. The extract is dried over magnesium sulfate and filtered.
Hydrogen sulfide is passed through the filtrate and the resulting precipitate is filtered off. The fil-trate is concentrated in vacuo and the residue is purified by silica gel column chromatography to give (2S,4S)-1-p-methoxybenzylox~7carbonyl-2-p-methoxybenzy-loxycarbonylsulfamoylaminomethyl-4-mercaptopyrrolidine (1.56 g). Yield: 92.4~.
NMR ~ (CDC13) ppm: 2.42 to 2.58(m, lH), 3.80(s, 6H), 5.08, 5.02(ABq, J=12Hz, 2H), 5.12, 5.07(ABq, J=16Hz, 2~7~

- 3~ -2~
IR ~ (CHC13)cm 1 3380, 1740, 1685, 1610.

Step 13. Preparation of an N-methyl compound To a solution of (2S,4S)-l-p-methoxybenzy-loxycarbonyl-4-tri-tylthio-2-(p-methoxybenzyloxycarbo-nylaminosulfon~laminomethyl)pyrrolidine (2.06 g:
2.63 mmole) in dimethylformamide (15 ml), a solution of lM-lithium bis(trimethylsilyl)amide in tetrahydrofuran (2.76 ml: 1.05 eq.) is added with stirring under ice, cooling. After stirring for 1 hour, iodomethane (491 ,ul: 3 eq.) is added. The mixture is stirred at the same temperature for 3 hou~s. The reaction mixture is poured into a mixture of ethyl acetate and aqueous sodium sulfite and the ethyl acetate layer is taken.
The organic layer is successively washed wi-th water an~
brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography (toluene : ethyl aceta-te - 4:1) to give (2S,4S)-l-p-methoxybenzyloxycarbonyl-4-trityl-thio-2-(N-p-methoxybenzyloxycarbonyl-N-methylaminosulfonyl)-aminomethylpyrrolidine (1.51 g). Yield: 72%.
NMR ~ (CDCl3) ppm: 1.4 to 1.6(m, lH), 1.9 to 2.1(m, lH), 2.5 to 3.3(m, 4H), 3.23(s, 3H), 3.5 to 3.8(m, lH), 3.76(s, 3H), 3.81(s, 3H), 4.93(ABq,J=10.4Hz, 2H), 5.10(ABq, J=15.2Hz, 2H), 6.35 to 6.55(m, lH), 6.8 to 7.5(m 23H).
IR ~ (CHCl3) cm 1 1727, 1695.

Step 14. Preparation of a mercapto compound by depro-tection To a solution of (2S,4S)-l-p-methoxybenzy-loxycarbonyl-4-tritylthio~2-(N-p-me-thoxybenzyloxycarbo-2V7~3~

nyl-N-methylaminosul-~onyl)arninomethylpyrrolidine (1.5 g: l.88 mmole) in a mixture of dichloromethane (4 ml) and methanol (10 ml), a solution of pyridine (381 ~ l: 2.5 eq.) and silver nitrate (640 mg: 2 eq.) in water (6 ml) is added with st:irring under ice cool-ing. The mixture is stirred at the same temperature for 30 minutes. The reaction mixture is dilu-ted with dichloromethane, washed with water, dried over mayne-sium sulfate, and concentrated in vacuo to about 5 ml.
The residue is dissolved in methanol (10 ml~ and hydro-gen sulfide is bubbled through it. The mixture freed from solid by filtering is concen-tra-ted in vacuo. The residue is purified by silica gel column chroma-tography (toluene : ethyl acetate = 2:1) to give (2S,4S)-l-p-methoxybenzyloxycarbonyl-4-mercapto-2-(N-p-methoxyben~
zyloxycarbonyl-N-methylaminosulfonyl)aminomethylpyrro-lidine (866 mg). Yield: 83~.
NMR ~ (CDCl3) ppm: 1.6 to 1.8(m, lH), 2.3 to 2.6(m, lH), 2.9 to 3.4(m, 5H), 3.3(s, 3H), 3.8(s, 6H), 3.8 to 4.2(m, lH), 6.3 to 6.6(m, lH), 6.88(d, J=8.6Hz, 2H), 7.2 to 7.4(m~ 2H).
IR ~ (CHCl3) cm~l: 1690.

207~

Peparative Example 2 of a pyrrolidlne derlvative HQ~ MsO~ Tr ~
~NI ~ Me 1 ~N, ~ Me 2 'N,' ~OOMe 3 ~ Pnz p~
TrS Tr ~ , T
N
Pnz Pnz Prlz TrS~ rS~

6 ~N ~ NH2 7~~N' ~ NHS02N~
Pn~ Pnz TrS~__ 8 ~N ~ ~ Ntl2g ~ ~2NH2 Pnz Pnz Step 1 Preparation of an O-mesyl compound To a solution of (2S,4R)-l-p-nitrobenzyloxy-carbonyl-4-hydroxypyrrolidine-2-carboxylic acid methyl ester (59.44 g: 0.183 mole) in dichloromethane (150 ml) stirring at -20~C, triethylamine (30.5 ml: 1.2 eq.) and methanesulfonyl chloride (17 ml: 1 eq.) are added. The mixture is stirred at the same temperature for 35 minutes. To the mixture is added ice wa-ter and ethyl acetate. The organic layer is taken, washed with water, dried over magnesium sulfate, and concentrated in vacuo to give (2S,4R)-l-p-nitrobenzyloxyarbonyl-4-methanesulfonyloxypyrrolidine-2-carboxylic acid methyl ester (74.05 g). Yield: Quantitative.

21~ r~ 3 ~1 ~MR ~ (CDCl3) ppm: 2.20 to 2.42(m, lH), 2.55 to 2.85(m, lH), 3.07(s, 3H), 3.67(s, 1.5H), 3.78~s, 1.5H), 3.80 to 4.05(m, 2H), 4.53(t, J=7Hz, lH), 5.06 -to 5.40(m, 3H), 7.47(d, J=9Hz, lH), 7.51(d, J=9Hz, 1~), 8.23(d, J=9Hz, 2H).
IR ~ (CHCl3) cm 1 1748, 1712, 1608.

Step 2. Preparation of a tritylthio compound To a solution of tritylmercaptan (37.69 g:
10 1.5 eq.) in te-trahydro~uran (180 ml), an oil sl1spen-sion containing 60~ sodium hydride (4.73 g: 1.3 eq.) is added with stirring at 0~C. The mix-ture is stirred at room temperature overnight. ~ solution o~
(2S,4R)-1-p-ni.trobenzyloxycarhonyl~4-methanesulfonyl-15 oxypyrrolidine-2-carboxylic acid methyl ester (36.58 g:
90.9 mmole) in tetrahydro:Euran (180 ml) is added to the reaction mixture with stirrin~ at 0~C, and the mixture is stirred at 60~C for 30 mi.nutes. The reaction mix-ture is poured into cold dilute hydrochloric acid and 20 extracted with ethyl acetate. The extract is succes-sively washed with water and brine, dried, and concen-trated in vacuo. ~he residue is puri~ied by silica gel column chromatography (toluene : e-thyl acetate = 9:1 to 4:1) to give (2S,4S)-1-p-nitrobenzyloxycarbonyl-4-25 tritylthiopyrrolidine-2-carboxylic acid methyl ester (25.48 g). Yield: 48.1%.
NMR ~ (CDC13) ppm: 1.63 to 2.35(m, 2H), 2.68 to 3.50(m, 3H), 3.60(s, 1.5H), 3.72(s, 1.5H), 4.02 to 4.15(m, lH), 4.95 to 5.28(m, 2H), 7.10 to 7.52(m, 17H), 30 8.17(d, J=9Hz, lH), 8.24(d, J=9Hz, lH).
IR ~ (CHCl3) cm 1 1747, 1704, 1607.

207~3~

Step 3. Preparation of a methylol compound To a solution of (2S,4S)-l-p-nitrobenzyloxy-carbonyl-4-tritylthiopyrrolid:Lne-2-carboxylic acid methyl ester (5 g: 9.01 mmole) in tetrahydrofuran (180 ml) stirring under ice cooling, a solution o~
sodium borohydride (2.3 g: 1.4 eq.) in ethanol and a solution of lithium chloride (2.76 g: 1.5 eq.) in tetrahydrofuran (60 ml) are added. The mixture is stirred at room temperature for l hour. The reaction mixture is poured in-to a mixture of ice water and ethyl acetate and extracted with ethyl acetate. The extract is successively washed with cold dilute hydrochloric acid, aqueous sodium hydrogen carbonate, and saturated brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is recrystallized from methanol to give (2S,4S) 1-p-nitrobenzyloxycarbonyl-4-tritylthi-opyrrolidine-2-methanol (15.9 g). ~ield: 65.9~. mp.
122 to 125~C.
NMR ~ (CDC13) ppm: 1.32 to 1.53(m, 1~, 1.90 to 2.12(m, lH), 2.65 to 3.05(m, 3 H), 3.32 to 3.84(m, 3H), 5.08, 5.17(ABq, J=12Hz, 2H), 7.08 to 7.55(m, 17H), 8.26(d, J=9Hz, 2~).
IR ~ (CHC13) cm 1 3~00br, 1681, 1607.

Step 4. Preparation of a mesyl compound To a solution of (2S,4S)-1-p-nitrobenzyloxy-carbonyl-4-tritylthiopyrrolidine-2-methanol (5.0 g:
9.01 mmole) in di~hloromethane (50 ml) stirring at -15~C, triethylamine (1.63 ml: 1.3 eq.) and methanesul-fonyl chloride (0.85 ml: l.1 eq.) are added. The mix-ture is s-tirred at -15 to -10~C for 30 minutes. The reaction mixture is poured into water and e:tracted with dichloromethane. The extract is successively 2~76~3~

washed with dilute h~drochlor:ic acid, aqueous sodium hydrogen carbonate, and water, dried over magnesium sul~ate, and concentrated in vacuo. The residue is purified by silica gel column chromatography (toluene :
ethyl acetate = 9:1) to give (2S,4S)-1-p-nitrobenzy-loxycarbonyl-4-tritylthiopyrrolidine-2-methanol meth-anesulfonate (4.86 g). Yield: 85.2%.
NMR ~ (CDC13) ppm: 1.65 to 1.93(m, lH~, 2.00 to 2.26(m, lH), 2.68 to 2.92(rn, 3H), 2.96(s, 3H), 3.78 to 3.98(m, lH), 4.16 to 4.30(m, lH), 4.38 to 4.52(m, lH), 5.11(br s, 2H), 7.08 to 7.52(m, 17H), 8.24(d, J=9Hz, 2H).
IR ~ (CHCl3) cm 1 1699, 1606.

Step 5. Preparation of a phthalimido compound A solu-tion o~ (2S,4S)-1-p-ni~robenzyloxycar-bonyl-A-tritylthiopyrrolidine-2-methanol methanesulfo-nate (4.39 g: 6.93 mmole) and potassium phthalimide (2.57 g: 2 eq.) in dimethylformamide (30 ml) is stirred at 70~C for 6 hours. Tne reaction mixture is poured into ice water and the precipitate is filtered off.
The precipitate is dissolved in ethyl acetate, washed with saturated brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography (toluene : ethyl acetate) to give (2S,4S)-l-p-nitrobenzyloxycarbonyl-2-phthalimidomethyl-4--tri-tylthiopyrrolidine (3.12 g).
Yield: 64.3%.
NMR ~ (CDCl3) ppm: 1.40 to 2.30(m, 2H), 2.60 to 3.08(m, 2H), 3.10 to 3.40(m, lH), 3.55 to 4.23(m, 3H), 4.92, 5.06(ABq, J=12Hz, 2H), 7.08 to 7.50 (m, 17H), 7.60 to 7.82(m, ~H), 8.10(d, J=9Hz, lH), 8.19(d, J=9Hz, lH).

2 a ~

IR ~ (CHC13) cm : 1720, 1701, 1607.

Step 6. Xemoval of a phthalyl group To a solution of (2S,4S)-l-p-nitrobenzyloxy-carbonyl-2-phthalimidomethyl-~-tritylthiopyrrolidine (10.46 g: 15.31 mmole) in a mixture of dichloromethane (30 ml) and methanol (160 ml), hydrazine hydrate (1.53 ml: 2 eq.) is added, and the mixture is concen-trated to remove clichlorome-thane by warming and re-fluxed for 3 hours and 15 mlnutes. The reaction mixture is concentrated in vacuo. The residue is diluted with dichloromethane and filtered to remove solid. The filtrate is washed with water, dried over magnesium sulfate, and concentrated in vacuo to give crude (2S,4S)-1-p-nitrobenzyloxycarbonyl-2-aminomethyl-4-tritylthiopyrrolidine (7.71 g). Yield: 91%.
NMR ~ (CDC13:CD30D=2:1) ppm: 1.46 to 3.76(m, lOH), 5.04, 5.12(ABq, J= 15Hz, 2H), 7.10 to 7.56(m, 17H), 8.12 to 8.30(m, 2H).
IR ~ (CHCl3) cm~1: 1695, 1606.

Step 7. Preparation of an N-sulfamoyl compound A solution of (2S,4S)-1-p-nitrobenzyloxycar-bonyl-2-aminomethyl-4-tritylthiopyrrolidine (4.7 g:
8.49 mmole) is dissolved in dichloromethane (45 ml) and cooled to a temperature of -70~C. To the mixture, a solution of diisopropylethylamine (3.4 ml: 2.3 eq.) and lM t-butoxycarbonylaminosulfonyl chloride (prepared from chlorosulfonyl isocyanate and -t-butanol before hand) in dichloromethane (21 ml), and the mi~ture is stirred for 1 hour and diluted with ice water. I'he reaction mixture is successively washed with dilute hydrochloric acid and aqueous sodium hydroyen carbon-2 ~ 3-~J

- 41 ~

a-te, dried over magnesium sulfate, and concentrated.
The residue ~s purif:Led by silica gel column chromatog-raphy (toluene : ethyl acetate) to give (2S,4S)-l-p-nitrobenzyloxycarbonyl-2-t-buto~ycarbonyl-aminosulfo-nylaminornethyl-4-tritylthiopyrrolidine (1.49 g~.
Yield: 24%.
NMR ~ (CDCl3) ppm: 1.40 to 2.30(m, 2H), 1.44(s, 9}l), 2.60 to 3.40(m, 5H), 3.71 to 3.95(m, lH), 5.08, 5.13(ABq, J=12Hz, 2H), 6.27(br s, lH), 7.07 to 7.55(m, 17H), 8.21(d, J=7Hz, lH), 8.26(d, J=7Hz, lH).
IR ~ (CHC13) cm~l: 3390, 1737, 1695, 1606.

Step 8. Removal of a Boc group To a solution of (2S,4S)-l-p-nitrobenzyloxy-carbonyl-2-t-butoxycarbonylaminosulfonylaminomethyl-4-tritylthiopyrrolidine (1.46 g: 2 mmole) in dichloro-methane (5 ml) under ice cooling, anisole (2.4 ml) and trifluoroacetic acid (3.9 ml) are added. The mixture is stirred at room temperature for 2 hours. The reac-tion mixture is diluted with ethyl acetate and ice water and extracted with ethyl acetate. The extract is successively washed with water and saturated brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is recrystallized from n-hexane to give (2S,4S)-l-p-nitrobenzyloxycarbonyl-2-sulfamoyl-aminomethyl-4-tritylthiopyrrolidine (1.4 g). Yield:
Nearly quantitative.
NMR ~ (CDC13) ppm: 1.43 to 1.70(m, lH), 2.08 to 2.30(m, lH), 2.65 to 3.50(m, 5H), 3.74 to 4.00(m, lH), 5.03, 5.13(ABq, J=15Hz, 2H), 5.73(br s, lH), 7.00 to 7.60(m, 17H), 8.25(d, J-9Hz, 2H).
IR ~ (CHCl3) cm 1 3334br, 1688, 1607.

~0~3~

Step 9. Preparation of a mercapto compound by deprotec-tion To a solution of (2S,9S)-l-p-nitrobenzyloxy-carbonyl-2-sulfamoylaminomethyl-4-tritylthiopyrrolidine (668 mg: 0.95 mmole) in tetrahydrofuran (6 ml), a solution of pyridine (0.254 ml: 2.7 eq.) and silver nitrate (403 mg: 2.5 eq.) in water (2 ml) is added under ice cooling. The mixture is stirred at room temperature for 1 hour. The reaction mixture is dilut-ed with dichloromethane (3 ml) and methanol (3 ml), and hydrogen sulfide is bubbled through it under ice cool-ing for 10 minutes. The resulting precipitate is removed hy filtering. The filtrate is diluted with dichloromethane, washed with water, dried over magnesi-um sulfate, and concentrated in vacuo. The residue is purified by silica gel column chroma-tography (toluene :
ethyl acetate) to give (2S,4S)-l-p-nitrobenzyloxycarbo-nyl-2-sulfamoylaminomethyl-4-mercaptopyrrolidine (233 mg). Yield: 63~.
NMR ~ (CDCl3-CD30D) ppm: 1.42(t, J=7Hz, lH), 1.65 to 1.93(m, lH), 2.48 to 2 .70(m, lH), 3.05 to 3.63(m, 4H), 3.93 to 4.16(m, 2H), 5.22(s, 2H), 7.53(d, J=8Hz, 2H), 8.23(d, J=8Hz, 2H).
IR ~ (CHC13) cm 1 3276hr, 1692, 1607.

~7S~3'~
- ~3 -Preparative Example 3 of a pyrrolidine derivative MsO~. ~sO~, MsOI~, Me 1 ~ H 2 ~ Ts 3' Pnz Pnz Pnz MsO~ A ~ ~
~t4 ~N, ~ ~ ~ ~ ~ NH2 6 Pnz Pnz Pnz HS_ ~NJ~ 2NH2 Pnz , Step 1. Preparation o e a methylol compound To a solution of (2S,4R)~1-p-nitroben~yloxy-carbonyl-4-methanesulfonyloxypyrrolidine-2-carboxylic acid methyl ester (79.4 g: 0.197 mmole) in a mixture of ethanol (300 ml) and tetrahydrofuran (150 ml), sodium borohydride (10.44 g: 1.4 eq.) is added in small por-tions with stirring at 0~C. The mixture is stirred at 0~C for 1.5 hours and at room temperature for 5 hours.
To the reac~ion mixture under ice cooling, 5N-hydro-chloric acid (100 ml) is added. The mixture is diluted with water, and extracted with ethyl acetate. The extract is washed with brine, dried over sodium sul-fate, and concentrated in vacuo. The residue is re-crystallized from a mixture of dichloromethane and ether to give (2S,4R)-1-p-nitrobenzyloxycarbonyl-4-methanesulfonyloxypyrrolidine-2-methanol (51.9 g).
-Yield: 70%.
NMR ~ (CDCl3) ppm: 1.93 to 2.14(m, lH), 2.32 to 2.48(m, lH), 3.06(s, 3H), 3.53 to 4.28(m, 6H), 5.26(s, 2H), 7.53(d, J=9Hz, 2H), 8.24(d, J=9Hz, 2H).

2 ~ 3 ~

- ~4 -IR ~ (CHCl3) cm : 3404, 1698, 1607.

Step 2. Preparation of a tosyl compound To a solution of (2S,4R)-1-p-nitrobenzyloxv-carbonyl-4-methanesu].fonyloxypyrrolidine-2 methanol (28.8 g: 77 ~mole) in dichlorome-thane (150 ml) under ice cooling, p-toluenesulfonyl chloride (l9.11 g:
1.3 eq.), triethylamine (10.4 ml: 1.3 eq.) and dimeth-ylaminopyridine (0.94 g: 0.1 eq.) are added. The mixture is stirred at 25~C for 7 hour.s. The reaction mixture is diluted with ice water. The resultant organic layer is taken, successively washed with aque-ous sodium hydrogen carbonate and water, dried over magnesium sulfate, and concentrated in vacuo. The residue is recrystallized from n-hexane to give (2S,4R)-1-p-nitrobenzyloxycarbonyl-4-methanesulfonyl-OXY~YL,olidine-2-methanol p-toluenesulfonate (37.7 g).
Yield: 93%.
NMR ~ (CDCl3) ppm: 2.20 to 2.50(m, lH), 2.44(s, 3~), 3.05(s, 3H), 3.45 to 4.60(m, 5H), 5.18(s, 2H), 5.26(br s, lH), 7.34(d, J=8Hz, 2H), 7.50(d,J=~Hz, 2H), 7.75(d, J=8Hz, 2H), 8.23(d, J=8Hz, 2H).
IR ~ (CHCl3) cm~1: 1700, 1599.

Step 3. Preparation of a phthalimido compound A mixture Gf (2S,4R)-1-p-nitrobenzyloxycarbo-nyl-4-methanesulfonyloxypyrrolidine-2-methanol p-toluenesulfonate (25 g: 47.3 mmole) and potassium phthalimide (17.52 g: 2 eq.) in dimethylformamide (250 ml) is stirred at 60~C for 7 hours. The reaction mixture is poured into ice water and filtrated. The resulting precipitate is dissolved in ethyi acetate, washed with saturated brine, dried over magnesium 2 0 ~

- ~5 -sulfate, and concentrated in vacuo. The re~idue is recrys-tallized from methanol to g:ive (2S,4R)-1-p-nitro-benzyloxycarbonyl-2-phthalimidomethyl-4-methanesulfony-loxypyrrolidine (18.76 g). Yield: 79~, mp. 121 to ~23~C.
NMR ~ (CDCl3) ppm: 2.03 to 2.60(m, 2H), 3.02(s, 3H), 3.50 to 4.15(m, 4H), 4.40 to 4.63(m, lH), 5.10, 5.29(ABq, J=15Hz, 2H), 5.10 to 5.30(m, lH), 7.46 (d, J=9Hz, lH), 7.57(d, J-9Hz, lH), 7.63 to 7.88(m, 4H), 8.20(d, J=9Hz, 2H) IR ~ (CHCl3) cm 1 1773, 1715, 1605.

Step 4. Preparation of an acetylthio compound A solution of (2S,4R)-l-p-nitrobenzyloxycar-bonyl-2-phthalimidomethyl-4-methanesul~onyloxy~Lloli-dine (10 g: 19.88 mmole) and potassium thioacetate (4.54 g: 2 eq.) in dimethylformamide (60 ml) is stirred at 60~C for 3 hours. The reaction mixture is poured into ice water (200 ml) and filtered. The precipitate is dissolved in ethyl acetate, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography (toluene :
ethyl acetate) to give (2S,4S)-1-p-nitrobenzyloxycarbo-nyl-2-phthalimidomethyl-4-acetylthiopyrrolidine (8.7 g). Yield: 90~.
NMR ~ (CDCl3) ppm: 1.65 to 1.97(m, lH), 2.47 to 2.67(m, lH), 3.24 to 3.34(q, lH), 3.73 to 4.24(m, 4H), 4.30 to 4.54(m, lH), 5.02(dd', J=14Hz, Js7Hz, lH), 5.20(d, J=14Hz, lH), 7.42(d, J=9Hz, lH), 7.45(d, Js9Hz, lH), 7.60 to 8.86(m, 4H), 8.17(d, J=9Hz, 2H).
IR ~ (CHCl3) cm 1 1773, 1714, 1605.

2 ~ 3 ~

- ~6 -Step 5. Removal of a phthalyl and an acetyl groups To a solution of (2S,4S)-1-p-nitrobenzyloxy-carbonyl-2-phthalimidomethyl-4--acetylthiopyrrolidine (4.92 g: 10.18 mmole) in a mixtu~e of dichloromethane (15 ml) and methanol (75 ml), hydrazine hydrate (1.53 ml: 3 eq.) is added. The mixture is warrned to remove dichloromethana and heated to reflux for 1 hour and 10 minutes. The reaction mixture is concentrated in vacuo. The residue is diluted with dichloromethane and filtered. The filtrate is washed with water, dried over magnesium sul~ate, and concentrated in vacuo to give crude (2S,4S)-1-p-nitrobenzyloxycarbonyl-2-ami.nomethyl-4-mercaptopyrrolidine (3.3 ~). Yield:
Quantitative.
NMR ~ (CDC13) ppm: 1.63 to l.90(m, lH), 2.48 to 2.68(m, 1~), 2.86 to 3.43(m, 4H), 3.65 to 4.23(m, 2H), 5.22(s, 2H), 7.52(d, J=9Hz, 2H), 8.23 (d, J=9~z, 2H).

Step 6. Preparation of an N-sulfamoyl compound To a solu~ion of crude (2S,4S)-1-p-nitroben-zyloxycarbonyl-2-aminomethyl-4-mercaptopyrrolidine (3.3 g: 10.18 mmole) in dichloromethane (100 ml) at -78~C, triethylamine (2.84 ml: 2.2 eq.) and trimethyl-chlorosilane (3.12 ml: 2.2 eq.) are dropwise added.
After stirring for 20 minutes, triethylamine (4.25 ml:
3 eq.) and lM-sulfamoyl chloride in dichloromethane (25 ml: 2.5 eq.) are dropwise added to -the mixture.
After 20 minu-tes stirring, the reaction mixture is acidified with hydrochloric acid, warmed to room tem-perature, and extracted with dichloromethane. The extrac-t is washed with water, and lN-hydrochloric acid (10 ml) and methanol (30 ml) are added thereto. The solution is stirred at room temperature for 30 minutes.

2 o r~ 3 ~) - ~7 -The reaction mixture is washed with water, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography (toluene : ethyl acetate) to give (2S,4S)-l-p-nitro-benzyloxycarbonyl-2-sulfamoylaminomethyl-4-mercaptopyr-rolidine (2.65 g). Yield: 66.7~.
NMR ~ (CDCl3-CD30D) ppm: 1.42(t, J=7Hz, lH), 1.65 to 1.93(m, lH), 2.48 to 2.70(m, lH), 3.05 to 3.63(m, 4H), 3.93 to 4.16(m, 2H), 5.22(s, 2H), 7.53(d, J=8Hz, 2H), 8.23(d, J=8Hz, 2H).
IR ~ (CHCl3) cm~1: 3276br, 1692, 1607.

Preparative Example 4 of a pyrrolidine derivative H0~ H0~. MsO~
COOH 1 1 ~ H 2 I~ OOMe 3 Boc ~oc Ms~ Ms~. Ms~.r__ ~N ~ ~ rN ~ Ts 5~ 'N ~ NFt 6 Boc Boc Boc Nl ~ 7 ~NI, ~ NH2 8~ ~'N ~ N~~2NH2 Boc ' Boc Boc Step 1. Preparation of a Boc compound To a suspension of trans-4-hydroxy-L-proline (50 g) in a mixture of water (300 ml) and t-butanol (100 ml) are added aqueous sodium hydrogen carbonate (32.3 g), di-t-butyl dicarbonate (104 g) and dioxane 2 ~ 3 ~

- ~8 -(200 ml). The mixture is stirred at room temperature overnight. The organic solvent i5 removed and the resulting aqueous solu-tion is layered with methyl ethyl ketone and ethyl acetate, and acidified with conc.
hydrochloric acid (3~.5 ml) under ice cooling. The organic layer is taken, washed wi-th saturated brine, dried over sodium sulfate, and concentrated in vacuo.
The residue is recrystallized from ethyl acetate-tol-uene to give trans-l-t-butoxycarbonyl-~-hydroxy-L-proline (82.9 g). Colorless crystals. Yield: 94~.
mp. 126 to 128~C.
NMR ~ (CDC13) ppm: 1.~3, 1.~6(2 x s, 9~), 1.95 to2.36(m, 2H), 3.36 to 3.6(m, 2~), 4.23 to ~.~4(m, 2H).
IR ~ (CHCl3) cm~1: 3360, 1735, 16S6.
Elemental analysis (C1oH17N05) Calcd.: C, 51.94; H, 7.41; N, 6.06.
Found : C, 51.65; H, 7.38; N, 5.99.

Step 2. Preparation of a compound having mesyloxy and methoxycarbonyl groups To a solution of trans-1-t-butoxycarbonyl-4-hydroxy-L-proline (3.5 g) in tetrahydrofuran (110 ml) at -30~C, triethylamine (12.8 ml) and methanesulfonyl chloride (6.27 ml) are added. The mixture is stirred at the same temperature for 30 minutes. To the mixture triethylamine (5.13 ml) and methanol (30 ml) are added.
The mixture is stirred for 30 minutes. The reaction mixture is acidified with lN-hydrochloric acid (37 ml) and extracted with ethyl aceta-te. The extract is successively washed with water, aqueous sodium hydrogen carbonate, water and saturated brine, dried over sodium sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography and re-2~7643~J

crystallized from toluene--petroleum ether to yive (2S,4~)-1-t-butoxycarbonyl-4-metharlesulfonyloxypyrroli--dine-2-carboxylic acid methyl ester (9.16 g). Colorless crystals. Yield: 77~. mp. 86 to 87~C.
NMR ~ (CDCl3) ppm~ 2, 1.47, 1.50(3 x s, 9H), 2.19 ~o 2.35(m, lH), 2.48 -to 2 .75(m, lH), 3.06, 3.07, 3.26(3 x s, 3H), 3.59 to 4.12(m, 5H), 4.35 to 4.60(m, lH), 5.18 to 5.32(m, lH).
IR ~ (CHCl3) cm~1: 1748, 1698.
Step 3. Preparation of a methylol oompound To a solution of (2S,4R)-1-t-butoxycarbonyl 4-methanesulfonyloxypyrrolidirle-2-carboxylic acid methyl ester (8.11 g) in -tetrahydrofuran (49 ml) stir-ring under ice cooling, sodium borohydride (2.36 g) and rnethanol (20 ml) are added. The mixture is stirred at room temperature for 25 minu~es and at 60~C for 25 minutes. The mixture is cooled with ice and fil-tered. The filtrate is concentrated, diluted with ethyl acetate, washed with water, dried over sodium sulfate, and concentrated in vacuo. The residue is recrystallized from petroleum ether-ether to give (2S,4R)-1-t-butoxycarbonyl-4-methanesulfonyloxypyrroli-dine-2-methanol (5.96 g). Colorless crystals. Yield:
80~. mp. 95 to 96~C.
NMR ~ (CDCl3~ ppm: 1.48(s, 9H), 1.78 to 2.02(m, lH), 2.3 to 2.48(m, lH), 3 .05(s, 3H), 3.5 to 3.65(m, 2H), 3.65 to 4.0(m, 2H), 4.03 to 4.25(m, lH), 5.2(s , lH).
IR ~ (CHC13) cm~l: 3460, 1680.
Step 4. Preparation of a tosyl compound To a solution of (2S,4R)-1-t-butoxy~arbonyl-4-methanesulfonyloxypyrrolidine-2-methanol (12.0 g) in 20~3~

dichloromethane (1~0 ml) stirring under ice cooling, triethylamine (6.23 ml), p--toluenesul~onyl chloride (8.52 g) and N, N-dimethylaminopyridine (993 mg) are successively added. The mixture is heated to reflux for 3 hours, supplemented with trie~hylamine (0.57 ml) and p-toluenesulfonyl chloride (775 mg), and heated to reflux for 1 hour. The reaction mixture is acidified with dilute hydrochloric acid. The organic layer is taken, washed with water, dried over sod.ium sulfate and concentrated in vacuo. The residue is purified by silica gel column chromatography and recrystallized from n-hexane to gi~e (2S,4R)-l~t-butoxycarbonyl-4~
me-thanesulfonyloxypyrrolidine-2-methanol p-toluenesul-fonate (16.8 g). Yield: 92%. mp. 65 to 66~C.
NMR ~ (CDC13) ppm: 1.42(s, 9H), 2.15 to 2.55(m, 2~), 2.45(s, 3~1), 3.03(s, 3H), 3.3 to 4.5(m, 5H), 5.1 to 5.25(m, lH), 7.35(d, J=8.0Hz, 2H), 7.76(d, J=8.0Hz, 2H)-IR ~ (CHCl3) cm 1 1693.
Step 5. Preparation of a phthalimido compound To a solution of (2S,4R)-1-t-butoxycarbonyl-4-methanesulfonyloxypyrrolidine-2-methanol p-toluene-sulfonate (20.78 g) in dimethylformamide(200 ml), potassium phthalimide (9.61 g) is added. The mixture is stirred at 70~C for 3 hours. The reaction mixture is poured in-to a mixture of water and ethyl acetate.
The organic layer is taken, successively washed w~th dilute aqueous sodium hydroxide and water, dried over sodium sulfate, and concentrated in vacuo. The residue is purified by 5~ wet silica gel column chromatography to give (2S,4R)-1-t-butoxycarbonyl-2-phthalimidomethyl-4-methanesulfonyloxypyrrolidine (11.17 g). Yield: 60%.

2~7~3~

- 5:l -Colorless foam.
N~R ~ (CDCl3) ppm: 1.33, 1.42(2 x s, 9H), 2.0 to 2.55(m, 2H), 3.02(s, 3H), 3.4 to ~.6(m, 5H), 5.15 to 5.3(m, lH), 7.6 to 7.95(m, 4H).
IR ~ (C~Cl3) cm 1 1775, 1716, 1693.

Step 6. Preparation o~ an acetyll,hio compound To a solution of (2S,4R)-l-t-butoxycarbonyl-2-phthalimidomethyl-4-methanesul:fonyloxypyrrolidine (3 g) in dimethylformamide (30 ml), potassium thioace-tate (1.65 g) is added. The mixture is stirred at 60~C
for 3.5 hours. The reac-tion mixture is poured into a mixture of e-thyl acetate and dilute hydrochloric acid.
The organic layer is taken, washed with w~ter, dried over sodium sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography to give (2S,4S)-l-t-bu~oxycarbonyl-2-phthalimido methyl-4-acetylthiopyrrolidine (2.12 g). Yield: 74~.
Orange colored syrup.
NMR ~ (CDCl3) ppm: 1.30, 1.3~(2 x s, 9H), 1.6 to 2.0(m, lH), 2.3~(s, 3H), 2.~ to 2.67(m, lH), 3.15 to 3.3(m, lH), 3.6' to 4.55(m, 5H), 7.6 to 8.0(m, 4H).
IR ~ (CHC13) cm~l: 1774, 1715, 1688.

Step i. Removal of a phthalyl and an acetyl groups To a solution of (2S,4S)-l-t-butoxycarbonyl-2-phthalimidomethyl-4 acetylthiopyrrolidine (8.58 g) in a mixture of dichloromethane (26 ml) and methanol (129 ml), hydrazine hydrate (~.11 ml) is added. The mixture is heate~ to reflux for 2 hours and 45 minutes and filtered. The filtrate is concentrated in vacuo.
The residue is dissolved in dichloromet.~ane, washed with water, dried over sodium sulfate, and concentrated ~7~31~

in vacuo to give crude (2S,4S)-1-t-butoxycarbonyl-2-aminomethyl-4-mercap-topyrrolidine (4.1 g). Yellow syrup.

Step 8 Preparation of a sulfamoyl compound To a solution of crude (2S,4S)-1-t-butoxycar-bonyl-2-aminome~hyl-4-mercaptopyrrolidine (4.1 g) in dichloromethane (250 ml) at -70~C under a stream of ni-trogen, triethylamine (8.87 ml) and trimethylchlo-rosilane (6.73 ml) are added. The mixture is stirredfor 1 hour and 40 minutes, mi~ed with triethylamine (8.B7 ml) and a solution of lM-sulfamoyl chloride in dichloromethane (64 ml), and stirred for 1 hour. The reaction mixture is acidified with dilute hydrochloric acid. The organic layer is taken, dilu-ted with lN-hydrochloric acid (21 ml) and methanol (50 ml), stirred for 35 minutes at room temperature, and poured into water. The organic layer is -taken, washed with water, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography to give (2S,4S)-l~t-butoxycarbony,-2-sulfamoylaminomethyl-4-mercaptopyrrolidine (4.57 g).
Yield: 69~. Colorless syrup.
NMR ~ (CDC13) ppm: 1.46(s, 9H), 1.5 to 1.8(m, lH), 1.71(d, J=6.6Hz, lH), 2.5 to 2.67(m, lH), 3.0 to 3.46(m, 4H), 3.85 to 4.2(m, 2H), 4.6 (br s, 2H).
IR ~ (CHC13) cm~1: 3420, 3340, 3270, 1679, ~0~3~3 Preparative Example 5 of a pyrrol:idine derivative MsO~ MsO~ MsO~
I ~ Mel l~ H 2 l~ Ts 3 Pn~ P~ Pmz ~N ~ NrL 4 ' ~N ~ NFt 5 ~ ~ 2 \ ~ /

~N~ 2NH2 Pmz Step. 1 Preparation of a methylol compound To a solution of (2S,4R)-l-p-methoxybenzyl-oxycarbonyl-4-methanesulfonyloxypyrrolidine-2-carboxyl-ic acid methyl ester (79.4 g: 205 mmole) in a mixture of tetrahydrofuran (200 ml) and ethanol (300 ml), sodium borohyride (14 g) is added in several portions under ice cooling. The mixture is stirred at room temperature for 4 hours. The reaction mixture is neutralized with conc. sulfuric acid, concentrated in vacuo to approximately a half volume, diluted with water, and extracted with ethyl acetate. The extract is successively washed with aqueous sodium hydrogen carbona-te, water and brine, dried over magnesium sul-fate, and concentrated in vacuo. The residue i3 puri-fied by silica gel column chromatography (toluene :
ethyl acetate = 1:2) to give (2S,4R)-l-p-.

2 ~ 3 ~3 - 5~ -methoxybenzyloxycarbonyl-~-methanesulfonyloxypyrroli-dine-2-methanol (58.7 g). Yield: 81.7%.
NMR ~ (CDCl3) ppm: 1.8 to 2.2~m, lH), 2.3 to 2.5(m, lH), 3.01(s, 3H), 3.57 (d, J=4.4Hz, lH), 3.64(d, J=4.4Hz, lH), 3.81(s, 3H), 3.82 to 4.3(m,3H), 5.09(s, 2H), 5.21(br s, lH), 6.89(d, J=8.8Hz, 2H), 7.31(d, J=8.8Hz,2H).

Step 2. Preparation of a tosyl compound To a solution of (2S,4R)-1-p-methoxybenzyl-oxycarbonyl-4-methanesulfonyloxypyrrolidine-2-methanol (8.7 g: 24.2 mmole) in dichloromethane(80 ml) are added triethylamine (4.05 ml), p-toluenesulfonyl chloride (5.08 g) and A-dimethylam:Lnopyridine (148 mg). The mixture is stirred at room temperature overnight. The reaction mixture is washed with water and brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel col~lmn chromatography (toluene : ethyl acetate = 1 : 1) to give (2S,4R)-l-p-methoxybenzyloxycarbonyl-4-methanesul-fonyloxypyrrolidine-2-methanol p-toluenesulfonate (11.75 g). Yield: 95~.
NMR ~ (CDCl3) ppm: 2.2 to 2.5(m), 2.44(s, 3H), 2.98(s, 3H), 3.4 to 3.6(m, 2H), 3.82(s, 3H), 3.8 to 4.6(m), 5.03, 4.95(A~q, J=12Hz, 2H), 5.2 (br s, lH), 6.89(d, J=8.6Hz, 2H), 7.18 to 7.4(m, 4H), 7.6 to 7.8(m, 2H).
IR ~ (CHCl3) cm~l: 1698, 1612.

3~ Step 3. Preparation of a phthalimido compound To a solution of (2S,4R)-1-p-methoxybenzyl-oxycarbonyl-4-methanesulfonyloxypyrrolidine-2-me-thanol p-toluenesulfonate (6.35 g: 12.27 mmole) in dimethyl-... .

2~7~3~

formamide (60 ml), potassium phthalimide (2.7 g) is added. The mixtllre is s-tirred at 70JC for 4 hours.
The reaction mixture is poured into ice water and e~tracted with ethyl ace-tate. The extract is succes-sively washed with water and brine, dried over magnesi-um sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography (toluene :
ethyl acetate = 2:1) to give (2S,4R)-1-p-methoxybenzy-loxycarbonyl-4-methanesulfonyloxy-2-phthalimidome-thyl-pyrrolidine (4.65 g). Yield: 77.5%.
NMR ~ (CDCl3) ppm: 2 to 2.3(m, lH), 2.4 to 2.6(m, lH), 2.95, 2.97(2 x s,3H), 3.43 to 4.2(m, 5H), 3.80(s, 3H), [5.01(s)+5.07, 4.96(ABq, 12.2Hz), 2H], 5.13 to 5.3(m, lH)-IR ~ (CHCl3) cm~1: 1774, 1716, 1613.

Step 4. Preparation of an acetylthio compound To a solution of (2S,4R)-l-p-methoxybenzyl-oxycarbonyl-4-methanesulfonyloxy-2-phthalimidopyrroli-dine (4.0 g: 8.19 mmole) in dimethylformamide (40 ml), potassium thioacetate (2.1 g) is added. The mixture is stirred at 60~C for 3 hours. The reaction mixture i5 diluted with ethyl acetate, successively washed with water and brine, dried over magnesium sulfate, ar.d concentrated in vacuo. The residue is purified by silica gel column chromatography (toluene : ethyl acetate =2:1) to give (2S,4S)-1-p-methoxybenzyloxycar-bonyl-4-acetylthio-2-phthalimidomethylpyrrolidine (3.Z g). Yield: 78~.
NMR ~ (CDCl3) ppm: 1.7 to l.9(m, lH), 2.4 to 2.7(m, lH), 3.21, 3.26(2 x d, ~=7Hz, 2H), 3.8(s, 3H), 3.7 to 4.2(m), 4.2 to 4.5(m, lH), [4.95(s) +5.04, 4.83(AB~, J=12Hz), 2H], 6.83(d, J=7.6Hz, 2H), 7.18 to 7.3(m, 2H), 207~

7.6 to 7.9(m, 4H).
IR ~ (CHCl3) cm~1: 1773, 1714.

St2p 5. Removal of an acetyl and a phthalyl ~roups an~
introduction of a sulfamoyl group To a solution of (2S,4S)-1-p-methoxybenzyl-oxycarbonyl-4-acetylthio-2-ph-thalimidomethylpyrrolidine (4.3 g: 9.18 mmole) in a mixture of dlchloromethane (13 ml) and methanol (65 ml), hyrazine hydrate (1.78 ml) is added. The mixture is heated to reflux for 4 hours. The reaction mix-ture is concentrated in vacuo. The residue is dissolved in dichloromethane and filtered under a stream of nitrogen -to remove solid.
The filtrate is washed with water, dried over magnesium sulfate, and concentrated in vacuo. The resulting residue containing (2S,4S)-1-p-methoxybe,nzyloxyc~rbo-nyl-4-mercapto-2-aminonethylpyrrolidine is diluted with dichloromethane (lO0 ml), added triethylamine (2.63 g) and trimethylchlorosilane (2.4 ml) at -78~C, and stirred for 20 minutes. To the reaction mixture, triethylamine (2.63 ml) and a solution (16.5 ml) of lM-sulfamoyl chloride in dichloromethane are added.
The mixture is stirred for 20 minutes. The reaction mixture is washed with dilute hydrochloric acid, mixed with lN-hydrochloric acid (9 ml) and methanol (20 ml), and stirred at room temperature for 30 minutes. The reaction mixture is successively washed with water and brine, dried over magnesium sulfa-te, and concentrated in vacuo. The residue is purified by silica gel column chromatography (toluene : ethyl acetate = 1:2) to give (2S,4S)-l-p-methoxybenzyloxycarbonyl-4-mercapto-2-sul-famoylaminomethylpyrrolidine (2.71 g). ~ield:78.6~.

2~7~3~

NMR ~ (CDC13) ppm: 1.6 to 2.0(m, 2H), 2.~ to 2.7(m, lH), 3.1 to 3.8(m,4H), 3.81(s, 3H), 3.9 to 4.2(m, 2H), 4.6 to 5.0(m, 2H), 5.04(s, 2H), 5.97(br s, lH), 6.89(d, J=8.6Hz, 2H), 7.30(d, J=8.6Hz, 2H).
IR ~ (CHC13) cm~l: 3668, 3424, 1683.

Preparative Example 6 of a pyrrolidine derivative H0~ H~ H0~. r T 3-1 ~ ~ H 1 rN ~ H 2 Nl ~ 4-1 Pmz P~ P~

rN ~ ~C 4 2 rN ~ NH2 - ~ ~ NHS02NH2 P~ P~ Pmz 3 1~ NPrc a N3 4-2~ NPro 8 NFt ¦ 5 4~3 Ms~
'~N'I~NHS02NH2 f 6 ~

~N~ 2 7 ~N' ~ ~2N~2 Pm~ Pmz .

Step 1. Preparation of a methylol compound To a solution of trans-l-p-methoY.,ben2yloxy-carbonyl-4-hydroxyproline (105.5 g: 357.5 mmole) in tetrahydro:'uran (1 liter), triethylamine (54.8 ml) is added. After adding ethyl chloroformate (35.9 ml) dropwise at -30~C, the mixture is stirred for 2~7~

~ 5B -20 minutes. To the reac-tion mixture, a solution of sodium borohyride (33.25 g) in water (120 ml) is added dropwise at a temperature in the range of -15 to -5~C, neutralized with conc. hydrochloric acid, and concen-trated in vacuo. To the residue, ethyl acetate isadded, washed with brine, dried over magnesium sul-fate, and concentrated in vacuo to give (2S,4R)-1-p-methoxybenzyloxycarbonyl-4-hydroxy-pyrrolidine-2-methanol.
NMR ~ (CDCl3) ppm: 1.6 to 1.8(m, lH), 1.95 to 2.2(m, lH), 3.4 to 3.~ (m, 4H), 3.8(s, 3H), 4.0 to 4.3(m, 1~), 4.37(br s, lH), 5.07(s, 2H), 6.88(d, J=8.8Hz, 2~), 7.30(d, J-8.8Hz, 2H).

Step 2. Preparation of a tosyl compound To a solution of (2S,4R)-1-p-methoxybenzyl-oxycarbonyl-4-hydroxypyrrolidine-2-methanol (64 g:
227.6 mmole) in pyridine (350 ml), toluene p-sul~onyl chloride (48 g) is added. The mixture is stirred at room temperature for 4 hours. The reaction mixture is poured into ica water and extracted with ethyl acetate.
The extract is successively washed wi-th water, dilute hydrochloric acid and aqueous sodium hydrogen carbon-ate, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel column chromatography to give (2S,4R)-1-p-methoxybenzyloxycar-bonyl-4-hydroxypyrrolidine-2-methanol p-toluenesulfo-nate (60 g).
NMR ~ (CDCl3) ppm: 2~0 to 2.4(m, 2H), 2.44(s, 3H), 3.3 to 3.7(m, 2H), 3.82(s, 3H), 3.9 to 4.6(m, 4H), 4.8 to 5.1(m, 2H), 6.88(d, J=8.6Hz, 2H),7.2 to 7.4(m, 4H), 7.6 to 7.8(m, 2H).
IR ~ (CHCl3) cm 1 3446, 1693.

2~7~

Step 3. Preparation of an amino precursor (-NProc =
azido) 1) To a solution of (ZS,4R)-l~p-methoxybenzyl-oxycarbonyl-4-hydroxypyrrolidine--2-me-thanol p-toluene-sulfonate (8.7 g: 20 mmole) in dime-thylformamide (60 ml), a solution of sodium azide (1.56 g) in water (6 ml) is added. The mixture is stirred at 80~C over-night. The reaction mixture is poured into ice wa-ter and extracted with ethyl acetate. The extract is successively washed with water and brine, dried o~er magnesium sulfate, and concentrated in vacuo to give crude (2S,4R)~ p-methoxybenzyloxycarbonyl-4-hyd~oxy-2-azidomethylpyrrolidine (5.6 g). Yield: 92%.
NMR ~ (CDCl3) ppm: 1.95 to 2.1(m, 2H), 3.2 to 3.~(m, 3H), 3.81(s, 3H),3.83 to 4.6(m, 3H), 5.07(s, 2H), 6.89(d, J=8.8Hz, 2H), 7.31(d, J=8.8Hz, 2H).
IR ~ (CHC13) cm 1 3420, 2100, 1689.

2) To a solution of the thus obtained (2S,4R)-l-p-methoxybenzyloxycarbonyl-4-hydroxy-2-azido-methylpyrrolidine (5.57 g: 18.18 mmole) in methanol (30 ml) are added, 5~ palladium on charcoal (560 my) and ammonium formate (2~3 g). The mix-ture is stirred at 45~C for 2 hours. The reaction mixture is diluted with dichloromethane (50 ml), filtered to remove the catalyst, and concentrated in vacuo. The residue is crystallized from a mixture of dichloromethane and ether and washed with ether to give (2S,4R)-1-p-methoxybenzyloxycarbonyl-4-hydroxy-2-aminomethylpyrro-lidine formate (4.28 g). Yield: 72~.
NMR ~ (CDCl3-CD30D) ppm: 1.6 to 1.82(m, lH), 2.1 to 2.3(m, ]H), 2.7 to 3.7(m , 4H), 3.81(s, 3H), 4.1 to ~.4(m, 2H), 5.04(s, 2H), 6.88(d, J=8.8 Hz, 2H), 7.28(d, ~07~3~
~ 60 -J=8.8Hz, 2H), 8.47(s, 1~l).
IR ~ (CHCl3) cm 1 3678, 3412, 1678, 1602.

Step 4. Introduction of a protected amino group, remov-al of the protection, and introduction of a sulfamoyl group (-NProc = phthalimido) 1) To a solution of (2S,4R)-1-p-methoxyben-zyloxycarbonyl-4-hydroxypyrrolidine-2-methanol p-tol-uenesulfonate (24 g: 55.1 mmole) in dimeth~lformamide (200 ml), potassium phthalimide (15.3 g) is added. The mixture is stirred at 80~C for 4 hours. The reactlon mixture is diluted with ethyl ace-tate, successively washed with water and brine, drled over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica ~el column chromatography (toluene :
ethyl acetate = 1:2) to give (2S,4R)-1-p-methoxy-benzyloxycarbonyl-4-hydroxy-2-phthalimidomethylpyrroli-dine (18.1 g). Yield: 80%.
NMR ~ (CDCl3) ppm: 1.9 to 2.2(m, 2H), 3.4 to 4.05(m, 5H), 3.80(s, 3H), 4.3 to 4.6(m, 2H), 4.8 to 5.1(m, 2H), 6.83(d, J=8.2Hz, 2H), 7.25(d, J=8.2Hz, 2H), 7.6 to 7.9(m, 4H).
IR ~ (CHCl3) cm 1 3458, 1773, 1712.

2) To a solution of (2S,4R)-1-p-methoxyben-zyloxycarbonyl-4-hydroxy-2-phthalimidomethylpyrrolidine (5.13 g: 12.5 mmole) in a mixture of dichloromethane (15 ml) and methanol (50 ml), hydrazine hydrate (1.0 ml) is added. The mixture is heated to reflux for 2 hours and concentrated in vacuo. The residue is diluted with dichloromethane, filtered to remove solid, washed with brine, dried over magnesium sulfate, and concentrated in vacuo to give a residue containing 2~76~3~

(2S,4R)-l-p-methoxybenzyloxycarbonyl-4-hydroxy-2-amino-methylpyrrolidine as a main component.

3) To a solution of the above-mentioned residue in dichloromethane (70 ml) at -70~C, triethyla-mine (4.6 ml) and trimethylchlorosilane (3.7 ml) are added. The mixture is stirred for 20 minutes. To the reaction mixture, triethylamine (5.5 ml) and a solution of lM-sulfamoyl chloride in dichloromethane (34 ml) are added. The mixture is stirred for 15 minutes.
The reaction mixture is washed with dilute hydrochloric acid, mixed with methanol (50 ml), and then 4N-hydrochloric acid (3.3 ml) is added under lce cooling. A~ter stirring the mixture, aqueous sodium lS hydrogen carbonate is added. The organic layer is taken, washed with water and brine, dried over magnesium sulfate, and concentrated in vacuo to give crude (2S,4R)-l-p-methoxybenzyloxycarbonyl-4-hydroxy-2-sulfamoylaminomethylpyrrolidine (3.96 g).
NMR ~ (CDC13) ppm: l.8 to 2.25(m, 2H), 3 to 4.5(m, 7H), 3.79(s, 3H), 5.03(s, 2H), 5.2 to 5.8(m, 2H), 6.08(br s, lH), 6.87(d, J=8.8Hz, 2H), 7.29(d, J=8.8Hz, 2H).
IR ~ (CHCl3) cm l 3456, 1689.
Step 5 Preparation of a mesyl compound To a solution of crude (2S,4R)-l-p-methoxy-benzyloxycarbonyl-4-hydroxy-2-sulfamoylaminomethylpyr-rolidine (l.8 g: 5 mmole) obtained in Step 4 in dichlo-romethane (20 ml) at -70~C, triethylamine (0.77 ml) and methanesulfonyl chloride (0.39 ml) are added. The mixture is stirred for 45 minutes. ~he reaction mix-ture is neutralized with dilute hydrochloric acid, ~a7~3~

successively washed with water ancl brine, and concen-trated in vacuo to give crude (2S,4R)-1-p-methoxybenzy-loxycarbonyl-4-methanesulfonyloxy-2-sulfamoylaminometh-ylpyrrolidine (2.26 g).
NMR ~ (CDCl3) ppm: 2 to 2.5(m, 2H), 2.99(s, 3H), 3.0 to 4.3(m, 5H), 3.79(s, 3H), 4.8 to 5.3(m, 3H), 5.05(s, 2H), 5.7 to 5.85(m, lH), 6.88(d,J=8.8Hx, 2H), 7.29(d, J=8.8Hz, 2H).
IR ~ (CHCl3) cm 1 3606, 3416, 1690.
Step 6. Preparation of an acetylthio compound To a solution of crude (2S,4R)-1-p-methoxy-benzyloxycarbonyl-4-methanesulfonyloxy-2-sulfamoylami-nomethylpyrrolidine (2.26 g) obtained in Step 5 in dimethylformamide (12 ml), potassium thioacetate (1.7 g) is added. The mixture is stirred at 60~C for 5 hours. The reac-tion mixture is diluted with ethyl acetate, successively washed with water and brine, dried over magnesium sulfate, and concentraved in vacuo. The residue is purified by silica gel column chromatography (toluene : ethyl acetate = 1 : 2) to give (2S,4S)-l-p-methoxybenzyloxycarbonyl-4-acetylthio-2-sulfamoylaminomethylpyrrolidine (971 mg).
NMR ~ (CDCl3) ppm: 1.8(br s, lH), 2.33(s, 3H), 2.4 to 2.7(m, lH)-, 3.1 to 3.5(m), 3.81(s, 3H), 3.9 to 4.2(m, 2H), 5.05(s, 2H), 6.89(d, J=8.8Hz, 2H), 7.30(d, J=8.8Hz, 2H).
IR ~ (CHCl3) cm 1 3414, 3276, 1688.

S-tep 7. Removal of an acetyl group To a solution of (2S,4S)-1-p-methoxybenzyl-oxycarbonyl-4-acetylthio-2-sulfamoylaminomethylpyrroli-dine (982 mg: 2.35 mmole) in a mixture of dichlorometh-~07~

ane (2 ml) a~d methanol (10 ml), lN-sodium hydroxide (2.8 ml) is added under ice cooling. The mixture is stirred for 15 minutes. The reaction mixture is dilut-ed with water and extracted with ethyl acetate. The extract is successively washed with water and brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is purifiecl by silica gel colurnn chroma~ography (toluene : ethyl aceta-te = 1:2) to give (2S,4S)-1-p-methoxybenzyloxycarbonyl-4-mercapto-2-sulfamoylaminomethylpyrrolidine (783 mg). Yield: 89%.
NMR ~ (CDCl3) ppm: 1.6 to 2.0(m, 2H), 2.4 to 2.7(m,lH), 3.1 to 3.8(m, 4H), 3.81(s, 3H), 3.9 to 4.2(m, 2H), 4.6 to 5.0(m, 2H), 5.04(s, 2H), 5.97(br s, 1 H), 6.89(d, J=8.6Hz, 2H), 7.30(d, J=8.6Hz, 2H).
IR ~ (CHCl3) cm~1: 3668, 3424, 1683.

Preparative Example 7-A of a pyrrolidine derivative HO."n 1 HO." 2 HO." 3 Nl 'COOH ~ COOMe ~'CO~l~e H H HCl Boc MsO." MsO. 5 AcS~
~'COOlle ~--OH ' ~ OH
Boc Boc Boc 6AcS~ Boc 7, ~, B~c ~ ~ NS02N~2 N ''--~0 Boc BG~

~07~3~
- 6~ -Step A-1. Preparation of an ester compound To a suspension of cis-4-hydroxy-D-proline (16.46 g: 125.5 mmole) in methanol (66 ml), thionyl-chloride (9.16 ml: 125.5 mmole) :is added in a nitrogen atmosphere under ice cooling, and the mixture is stirred at room temperature for 30 minutes. The mix-ture is further stirred to react at 40~C for 4 hours to give (2R,4R)-4-hydrox~-2-methoxycarbonylpyrrolldine hydrochloride as crude crystals (25.74 g). Yield:
113 ~. Colorless crystals.
NMR ~ (D20) ppm: 2.3 to 2.6(m, 2H), 3.33(s, lH), 3.4 to 3.5(m, 2H), 3.84(s, 3H), 4.6 to 4.7(m, 2H).
IR ~ (KBr) cm~1: 3320, 2980, 1728.

Step A-2. Preparation of a Boc compound To a suspension of (2R,4R)-4-hydroxy-2 methoxycarbonylpyrrolidine hydrochloride (25.64 g:
125 mmole) in dichloromethane (125ml), triethylamine (l9.llml: 137.5 mmole) is added dropwise in a nitrogen atmosphere under ice cooling. The mixture is stirred for 5 minutes at room temperature. Then, a solution of di-t-butyl dicarbonate (34.11 g: 156.3 mmole) in di-chloromethane (125 ml) is added dropwise, and -the mix-ture is stirred for 40 minutes at room temperature to give (2R,4R)-1-t-butoxycarbonyl-4-hydroxy-2-methoxycar-bonylpyrrolidine (26~85 g). Yield: 88%. Colorless crystals.
NMR ~ (C~C13) ppm: 1.46(d, J=8.4Hz, 9H), 2.0 to 2.2(m, lH), 2.2 to 2.5(m, lH), 3.4 to 3.8(m, 2H), 3.79(d, J=3.0~z, 3H), 4.2 to 4.5(m, 2H .
IR ~ (KBr) cm 1 3460, 1730, 1680.

.

, ~07~43~

Step A-3. Prepara-tion o~ a mesyl compound To a solution of (2R,4R)-1-t-butoxycarbonyl-4-hydroxy-2-methoxycarbonylpyrrolidine (9.81 g:
40 mmole) in dichloromethane (49 ml) in a nitrogen atmosphere under ice cooling, triethylamine (6.67 ml:
48 mmole) and methanesulfonyl chloride (3.70 ml:
48 mmole) are added. The mixture is stirred for 20 minutes to give (2~,4R)-l-t-butoxycarbonyl-4-methanesulfonyloxy-2-methoxyca:rbonylpyrrolidine as a crude oil (13.05 ~). Yield: 101%.
NMR ~ (CDCl3) ppm: 1.46(d, J=9.6Hz, 9H), 2.5(m, 2H), 3.02(s, 3H), 3.76(s, 3H), 3.8(m, 2H), 4.3 to 4.6(m, 1~l), 5.2 to 5.3(m, lH).

Step A-4. Preparation of a methylol compound To a solution of (2R,4R)-1-t-butoxycarbonyl-4-methanesulfonyloxy-2-methoxycarbonylpyrrolidine (11.21 g: 34.4 mmole) in a mixture of tetrahydrofuran (34 ml) and ethanol (51 ml), sodium borohydride (5.21 g: 137.7 mmoie) is added in a nitrogen atmosphere under ice cooling. The mixture is stirred for 75 minutes at room temperature to give (2R,4R)-1-t-butoxycarbonyl-4-methanesulfonyloxypyrrolidine-2-metha-nol (8.47 g). Yield: 83%. Colorless crystals.
NMR ~ (CDCl3) ppm: 1.48(s, 9H), 1.9 to 2.2(m, lH), 2.3 to 2.5(m, lH), 8.06 (s, 3H), 3.65(dd, J=11.2Hz, J=4.0Hz, lH), 3.5 to 3.9(m, 2H), 3.84(dd, J=11.2Hz, J=7.6Hz, lH), 4.1(m, lH), 5.2(m, lH).
IR ~ (KBr) cm 1 3490, 1688.
Step A-5. Preparation of an acetylthio compound (2R,4R)-l-t-Butoxycarbonyl-4-methanesulfony-loxypyrrolidine-2-methanol (i.e., a substrate) and 2 ~ .3 ~

potassium thioacetate (KSAc) are dissolved in dimethyl-formamide (DMF), and -the mixture is stirred. The conditions for this reaction are shown in Table 1, Step A-5. The reaction mixture is diluted with ethyl ace-tate, and ice wa-ter is added. The organic layer is taken, successively washed with aqueous sodium hydrox-ide, hydrochloric acid, water and saturated brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel chroma tography -to give (2R,4S)-4-acetylthio-1-t-buto~ycarbo-nylpyrrolidine-2-methanol.
NMR ~ (CDCl3) ppm: 1.47(s, 9H), 2.05(t, 2H), 2.34(s, 3~), 3.0 to 3.3(m, lH), 3.~0(dd, Jsll.6Hz, ~=5.2Hz, lH), 3.5 to 3.9(m, 3H), 3.9 to 4.2(m, 2H).
Step A~6. Introduction of a sulfamide group a) Production of N-t-butoxycarbonylsulfamide A solution of t-butanol (4.72 ml: 50 mmole) in ethyl acetate (100 ml) i5 cooled to -40~C, chlorosulfonyl isocyanate (4.35 ml: 50 mmole) is dropwise added there-to, and the mixture is stirred at -18~C for 20 minutes.
The reaction mixture is cooled to -72~C, gaseous ammo-nia (2 mole) is bubbled with stirring, and the mixture is stirred for 50 minutes while warming up to 10~C.
The reaction mixture is acidified with 5N-hydrochloric acid (30 ml) and the formed precipitate is filtered off. The organic layer is taken, successively washed with water and brine, dried over magnesium sulfate, and concentrated under reduced pressure. The crystalline residue is washed with hexane-ethyl acetate (1:5, 90 ml) and recrystallized from ethyl acetate-hexane to give N-t-butoxycarbonylsulfamide (8.81 g). Yield: 89%.
Colorless crystals. mp.130 to 131~C.

~7~3~

NMR ~ (C~3SOCD3) pprn : 1.43(s, 9H), 7.27(s, 2H).
IR ~ (Nujol) cm 1 3360 , 3270, 1718, 1548.
Elemental Analysis (C5H12N204S) Calcd.: C, 30.60; H, 6.17; N, 14.28; S, 16.34.
~ound : C, 30.39; H, 6.11; N, 14.30; S, 16.30.

b) Preparation o~ a sul~amide compoun~
To a solution of ~2R,4S)-4-acetylthio-1-t-butoxycarbo-nylpyrrolidine-2-methanol (i.e., a substrate) in tet-rahydrofuran (THF), triphenylphosphine (PPh3), N-t-butoxycarbonylsulfamide (BSMD), and azodicarboxylic acid diethyl ester (DEAD) are successively added under ice cooling. ~he conditions for this reaction are shown in Table 2, Step A-6. The reaction mixture is diluted with toluene, concentrated, diluted with tol-uene, and the formed crystals are filtered off. The ~iltrate is concentrated. The residue is purified by silica gel column chromatography -to give (2R,4S)-4-acetylthio-1-t-butoxycarbonyl-2-(N-t-butoxycarbonyl-N-sulfamoylamino)methylpyrrolidine.
NMR ~ (CDCl3) ppm: 1.41(s, 9H), 1.55(s, 9H), l.9 - 2.0(m, 2H), 2.35(s, 3H), 3.32(dd, J=11~4Hz, J=8.2Hz, lH), 3.6 - 3.9(m, 3H), 3.9 - 4.1(m, lH), 4.5(m, lH), 6.15(s, 2H).
IR ~ (KBr) cm 1 3420, 3320, 1706, 1686, 1666.

Step A-7. Removal of an acetyl group To a solution o~ (2R,4S)-4-acetylthio-1-t-butoxycarbonyl-2-(N-t-butoxycarbonyl-N-sulfamoyl-amino)methylpyrrolidine (i.e., a substrate) in dichlo-romethane, 4.92 M sodium methoxide (NaOMe) in methanol is added. The mixture is stirred. The conditions for this reaction are shown in Table 3, Step A-7. The . , .

207~
- 6-~ -reaction mixture is diluted with water. 'rhe ~ater layer is -taken, toluene is added thereto, and acidified with conc. hydrochloric acid under ice cooling. The organic layer is taken, successive]y washed with water and saturated brine, dried over magnesium sulfate, and concentrated in vacuo to give (2R,4S)-1-t-butoxycarbo~
nyl-2-(~-t-butoxycarbonyl-N-sulfamoylamino)methyl-4-mercaptopyrrolidine. mp. 90.0 to 91.5~C.
NMR ~ (CDCl3) ppm: 1.43(s, 9H), 1.52(s, 9H), 1.72(d, 10J=7.0Hz, lH), 1.9 to 2.0(m, 2H), 3.2 to 3.8(m, 5H), 4.5(m, lH), 6.11(s, 2H).
IR ~ (KBr) cm~l: 3220, 1698, 1683.
Elemental AnalysiS (C15H2906N3S2) Calcd. C:43.78, H:7.10, N:10.21, S:15.58.
15Found. C:43.55, H:7.11, N:10.37, S:15.75.

Preparative Example 7-B of a pyrrolidine derivative HO,., 1 H ~ 2 HO ~ 3 'CQOH ~ 'COOMe ~ 'COOMe Boc Boc ~c Ms ~ 4 Ms ~ 5 AcS-"
~N~J"~coOMe N~ H --} ~ OH
Boc Boc Boc 6 AcS.. , Bcc 7 HS... I Boc '' NS02NH2 ~ ' NSO ~H2 Boc Boc ~7~J~3~

Step B-1. Substitution for a formyloisy group To a solution of (2R,4R)-1--t-butoxycarbonyl-4-hydroxy-2-methoxycarbonylpyrrolidine (2.45 g:
10 mmole) in tetrahydrofuran (10 ml), a solution of formic acid (453 ~1: 12 mmole), triphenylphosphine (3.15 g: 12 mmole), and diethyl azodicarboxylate (1.89 ml: 12 mmole) in tetrahydrofuran (5.0 ml) ls added in a nitrogen atmosphere under ice cooling. The mixture is stirred for 30 minutes at the same tempera~
ture to give (2R,4S)-l-t-butoxycarbonyl-4~formyloxy-2-methoxycarbonylpyrrolidine (2.17 g). Yield: 79%.
Colorless oil.
NMR ~ (CDC13) ppm: 1.44(d, J=7.8Hz, 9H), 2.1 to 2.6(m, 2H), 3.5 to 3.9(m, 5H), 4.4(m, lH), 5.4(m, lH), 8.0(s, lH).

Step B-2. Removal of a formyl group To a solution of (2R,4S)-1-t-butoxycarbonyl-4-formyloxy-2-methGxycarbonylpyrrolidine (2.0~ g:
7.6 mmole) in methanol (21.0 ml), aqueous lN-sodium hydroxide (7.6 ml) is added under ice cooling. The mixture is stirred at the same temperature for 25 minutes to give (2R, 4S)-1-t-butoxycarbonyl-4-hydroxy-2-methoxycarbonylpyrroli.dine (1.86 g).
Yield: 100%. Colorless oil.
NMR ~ (CDC13) ppm: 1.44(d, J=9.2Hz, 9H), 1.9 to 2.4(m, 2H), 3.4 to 3.7(m, 2H), 3.74 (s, 3H), 4.3 to 4.6(m, 2H).

Step B-3. Preparation of a mesyl compound To a solution of (2R,4S)-1-t-butoxycarbonyl-4-hydroxy-2-methoxycarbonylpyrrolidine ~3.17 g:
12.9 mmole) in dichloromethane (16 ml) in a nitrogen 2~7~3~

atmosphere under ice cooling, -triethylamine (2.15 ml:
15.5 mmole) and methanesulfonyl chloride (1.19 ml:
15.5 mmole) are added. The mixture is stirred to react ~or 30 minutes to give (2R,4S)~ -butoxycarbonyl-4-methanesulfonyloxy-2-methoxycarbonylpyrrolidine as oil (4.13 g). Yield: 99%.
NMR ~ (CDC13) ppm: 1.46(d, J=8.4Hz, 9H), 2.3(rn, lH), 2.5 to 2.8(m, lH), 3.08(s, 3H), 3.8 to 4.0(m, 5H), 4~3 to 4.6(m, lH), 5.3(m, 1~).
Step B-4. Preparation o-f a methylol compound To a solution of (2R,4S)-1-t-butoxycarbonyl-4-methanesulfonyloxy-2-methoxycarbonylpyrrolidine (3.96 g: 12.2 mmole) in a mixture of tetrahydrofuran (12 ml) and ethanol (18 ml), sodium borohydride (1.85 g: 48.8 mmole) is added in a nitrogen atmosphere under ice cooling. The mixture is stirred for 45 m.inutes at room temperature to give (2R,4S)-l-t-butoxycarbonyl-4-methanesulfonyloxypyrrolidine-2-metha-nol (2.97 g). Yield: 83~. Colorless crystals. I~p. 95 to 96~C.
NMR ~ (CDC13) ppm: 1.49(s, 9H), 1.7 to 2.1(m, lH), 2.3 to 2.5(m, lH), 3.06(s, 3H), 3.4 to 3.7(m, 2H), 3.7 to 4.0(m, 2H), 4.0 to 4.3(m, lH), 5.2(m, lH).
IR ~ (K~r) cm~1: 3400, 3420, 1648.

Step B-5. Substitution for an acetylthio ~roup (2R,4S)-l-t-butoxycarbonyl-4-methanesulfonyl-oxypyrrolidine-2-m~thanol (i.e., a ~ubstrate) is al-lowed to react in the same manner as in Step A-5 in Preparative Example 7-A under a condition for S-tep B-5 shown in Table 1 to give (2R,4R)-4-acetylthio-1-t-butoxycarbonylpyrrolidine-2-methanol.

~7~3~

NM~ ~ ~CDC:L3) ppm: 1.47~s, 9H), 2.3~s, 3H), 2.4 to 3.2 (m, 2H), 3.58 to 4.1(m, 6H).
IR ~ (CHC13) cm 1 3380, 1690.

Step B-6. Introduction of a sulfamide group N-t-butoxycarbonylsulfamide is prepared in the same manner as in the paragraph (a) of Step A-6 in Preparative Example 7-A. (2R,4R)-4-acetylthio-1-t-butoxycarbonylpyrroli~ine-2-methanol (i.e., a sub-strate) is allowed to react with the obtained N-t-butoxycarbonylsulfamide i.n the simmilar manner ag in paragraph (b) of Step A-6 in Preparative Example 7-A
under a condition for Step B-6 shown in Table 2 to give (2R,4R)-4-acetylthio-l~t-butoxycarbonyl-2-(N-t-butoxycarbonyl-N-sulfamoylamino)methylpyrrolidine.
N~.R ~ (CDC13) ppm : 1.43(s, 9H), 1.53(s, 9H), 2.34(s, 3H), 2.5(m, lH), 3.15(dd, J=12.2Hz, J=6.2Hz, lH), 3.58(dd, J=lg.~Hz, J=3.2Hz, lH), 3.8 to 4.1(m, 2H), 4.16(dd, J=12.2Hz, J=7.8Hz, lH), 4.4 to 4.7 (m, lH), 6.11(s, 2H).
IR ~ (CHC13) cm 1 3360, 3200, 1710, 1688.

Step B-7. Removal o~ an acetyl group (2R,4R)-4-acetylthio-1-t-butoxycarbonyl-2-(N-t-butoxycarbonyl-N-sulfamoylamino) methylpyrrolidine (i.e., a substrate) is deacetylated in the similar manner as in Step A-7 of Preparative Example 7-A under a condition for Step B-7 shown in Table 3 to give (2R,4R)-l-t-butoxycarbonyl-2-(N-t-butoxycarbonyl-N
sulfamoylamino)methyl-4-mercaptopyrrolidine. mp. 92 to 93~C.
NMR ~ (CDC13) ppm : 1.2-1.5(m, lH), 1.42 (s, 9H), 1.54(s, 9H), 1.82(d, J=6.2Hz, lH), 2.5-2.7(m, lH), ~ 0 7 ~

4.09, 3.05 (A~X, J=12.0Hz, J=7.4Hz, J=8.2Hz, 2H), 4.06, 3.62(ABX, J=15.0Hz, J=10.8 Hz, J-3.2Hz, 2H), 4.2,-4.6(m, lH), 6.08(s, 2H).
IR ~ (CHCl3) cm 1 3380, 3220, 1718, 1680.

Preparative Example 7-C of a pyrrolidine derivative H0~ HO.. , 2 H0.. , ~ 3 H ~ ~Me Nl ~Me H H HCl Boc H ~ 4 H ~ 5 MsO~
Me'N, ~ Me ~ Me Boc Boc Boc 6 Ms ~ 7 AcS. , 8 N~H ' ~ H -' B~c Boc AcS", Boc 9 HS.. , Boc NSO2NH2 ~N~NS02NH2 Boc Boc Step C-1. Preparation of an ester compound To a suspension of trans-4-hydroxy-L-proline (200 g: 1.525 mole) in methanol (800 ml), acetylchlo-ride (163 ml: 2.288 mole) is added dropwise under ice cooling in a nitrogen atmosphre. The mixture is warmed to room temperature, mixed with thionyl chloride (55.7 ml: 0.763 mole), and stirred for 4 hours at 40~C
to give (2S,4R)-4-hydroxy-2-methoxycarbonylpyrrolidine hydrochloride (244.27 g). Yield: 88%. Colorless crystals.

20~6~3~

NMR ~ (D2O) ppm~ to 2.0(m, lH), 2.0 to 2.2(m, lH), 2.9 to 3.1(m, lH), 3.1~(cld, J=12.6Hz, J=3.6Hz, lH), 3.49(s, 3H), 4.2 to 4.4(m, 2H).
IR ~ (K~r) cm~1: 3380, 3330, 2695, 2960, 1742.

Step C-2. Preparation of a Boa compound To a suspension of (2S,4R)-4-hydroxy-2-methoxycarbonylpyrrolidine hydrochloride (12.71 g:
70 mmole) in dichlorometi-ane (70 ml), triethylamine (10.7 ml: 77 mmole) is added dropwise under ice coollng in a nitrogen atmosphere. The mixture is stirred for 5 min. at room temperature. A solution of di-t-butyl dicarbonate (19.10 g: 87.5 mmole) in dichloromethane (72 ml) is added dropwise thereto, and the mixture is stirred for 45 minutes at room temperature to give (2S,4R)-1-t-butoxycarbonyl-4-hydroxy-2-methoxycarbonyl-pyrrolidine (14.06 g). Yield: 82g6. Colorless oil.
NMR ~ (CDCl3) ppm: 1.44(d, J=9.6Hz, 9H), 1.9 to 2.4(m, 3H), 3.4 to 3.7(m, 2H), 3.74 (s, 3H), 4.3 to 4.6(m, 2H).

Step C-3. Substitution with a formyloxy group To a solution o~ (2S,4R)-1-t-butoxycarbonyl-4-hydroxy-2-metho~ycarbonylpyrrolidine (7.36 g:
30 mmole) in tetrahydrofuran (30ml), formic acid (1.36 ml: 36 mmole), triphenylphosphine (9.44 g:
36 mmole) and diethyl azodicarboxylate (5.67 ml:
36 mmole) are successively added in a nitrogen atmos-phere under ice cooling. The mixture is stirred to react for 40 minutes at the same temperature to give (2S,4S)-1-t-butoxycarbonyl-4-formyloxy-2-methoxycarbo-nylpyrrolidine (5.38 g). Yield: 66g6. Colorless crys-tals.

~7~;~3~

- 7~ -NMR ~ (CDCl3) ppm: 1.45(d, J-8.6Hz, 9H), 2.2 to 2.4(m, lH), 2.4 to 2.7(m, lH), 3.5 to 3.9(m, 2H), 3.75(s, 3H), 4.3 to 4.6(m, lH), 5.3 to 5.5(m, lH), '7.98 (s, lH).
IR ~ (KBr) cm l 3420, 1748, 1712, 1681.

Step C-4. Removal of a formyl group To a solution of (2S,4S)-1-t-butox~carbonyl~
4-formyloxy-2-methoxycarbonylpyrrolidine (5.12 g:
18.7 mmole) in methanol (51.0 ml), aqueous lN-sodiurn hydroxide (18.7 ml) is added under ice cooling.
The mixture is stirred at the sarne -temperature for 20 minutes to give (2S,4S)-1-t-butoxycarbonyl-4-hydroxy-2-methoxycarbonylpyrrolidine (4.09 g).
Yield: 89~. Colorless crystals.
NMR ~ (CDCl3) ppm: 1.44(d, J=8.2Hz, 9H), 2.0 to 2.2(m, lH), 2.2 -to 2.5(m, lH), 3.2 to 3.8(m, 3H), 3.79(d, J=2.8Hz, 3~), 4.2 to 4.5(m, 2H).
IR ~ (KBr) cm~1: 3460, 1728, 1677.
Step C-5. Preparation of a mesyl compound In a manner similar to Step A-3 in Prepara-tive Example 7-A, (2S,4S)-l-t-butoxycarbonyl-4-hydroxy-2-methoxycarbonylpyrrolidine in dichloromethane is mesylated with triethylamine and methanesulfonylchlo-ride in a nitrogen atmosphere under ice cooling to give (2S,4S)-1-t-butoxycarbonyl-4-methanesulfonyloxy-2-meth-oxycarbonylpyrrolidine. mp. 90.0 to 91.5~C.

Step C-6. Preparation of a methylol compound In a similar manner to that in Step A-4 in Production Example 7-A, (2S,4S)-1-t-butoxycarbonyl-4-methanesulfonyloxy-2-methoxycarbonylpyrrolidine is , ~

2~7G~3~

allowed to react to give (2S,4S)-I-t-butoxycarbonyl-4-methanesulfonyloxypyrrolidine-2-rnethanol.

Step C-7. Prepara-tion of an acetylthio compound (2S,4S)-1-t-butoxycarbonyl-4-methanesulfonyl-oxypyrrolidine-2-methanol (i.e., a substrate) is al-lowed to react in a similar manner to Step A-5 in Preparative Example 7-A under a condition for Step C 7 shown in Table l to give (2S,4R)-4-acetylthio-1-t-butoxycarbonylpyrrolidine-2-methanol.
NMR ~ (CDCl3) ppm: 1.47(s, 9H), 2.05 (t, 2H), 2.3A
(s, 3H), 3.0 to 3.3 (m, lH), 3.40(dd, J=11.6Hz, J=5.2Hz, lH), 3.5 to 3.9(m, 3H), 3.9 to 4.2(m, 2H).

S-tep C-8. Introduction of a sulfamide group N-t-butoxycarbonylsulfamide is prepared in -the same manner as in the paragraph (a) in Step A-6 in Preparative Example 7-A. (2S,4R)-4-Acetylthio-1-t butoxycarbonylpyrrolidine-2-methanol (i.e., a sub-strate) is allowed to react with N-t-butoxycarbonylsul-famide in the similar manner as in the paragraph (b) in Step A-6 in Production Example 7-A under a condition for Step C-8 shown in Table 2 to give (2S,4R)-4-ace-tylthio-l~t-bu~oxycarbonyl-2-(N-t-butoxycarbonyl-N-sulfamoylamino) methylpyrrolidine.
NMR ~ (CDC13) ppm: 1.41(s, 9H), 1.55(s, 9H), 1.~ to 2.0 (m, 2H), 2.35(s, 3H), 3.32(dd, J=11.4Hz, J=8.2Hz, lH), 3.6 to 3.9(m, 3H), 3.9 to 4.1(m, lH), 4.5(m. lH), 6.15(s, 2H).
IR ~ (KBr) cm 1 3420, 3320, 1706, 1686, 1666.

2~g~

Step C-9. R~moval of an acetyl group (2S,4~ 4-acetyl~hio-1-t-bu-toxycarbonyl 2-(N-t-butoxycarbonyl-N-sulfamoylamino) methylpyrrolidine (i.e., a substrate) is allowed to react in the similar manner as in Step A-7 in Preparative Example 7-A under a condltion for Step C-9 shown in Table 3 to gi.ve (2S,4R)-l-t-butoxycarbonyl-2-(N-t-butoxycarbonyl-N-sulfamoylamino) methyl-4-mercaptopyrrolidine. mp. 90.0 ~o 91.5~C.
NMR ~ (CDCl3) ppm: 1.43(s, 9H), 1.52(s, 9H), 1.72(d, J=7.0Hz, lH), 1.9 to 2.0(m, 2H), 3.2 to 3.8(m, 5H), A.5 (m, lH), 6.11(s, 2H).
IR ~ (KBr) cm~l: 3220, 1698, 1683.

Prepartiv~ Example 7-D of a pyrrolidine derivative HO." 1 HO." 2 HO.. , EtOCO 3 ~X)H I~COOH ~CCO
H Boc Boc HO--~ EtOCO 4 Ms~.,[ 5 Ac~
~a)o ~H ' ~N~OH
Boc Boc ~c 6 ACS~ ~ Boc 7 H~ Boc N, ~NSO~NH2 ~ ~NJ~NSO~NH2 ~oc Boc Step D-l. Preparation of an N-Boc compound To a suspension of trans-~-hydroxy-L-proline (50 g: 0.381 mole) in methanol (250 ml), a so ution of 4N-sodium hydroxide (95.4 ml: 0.381 mole) and di-t-207~3~

butyl dicarbonate (91.6 g: 0.42 mole) in methanol (55 ml) is added at -20~C. The mixture is stirred at 20~C for 3 hours. The reac-ti.on mixture is concen-trated and then dilu~ed with toluene (100 ml) and shaken. The aqueous layer is taken, and mixed with conc. hydro-chloric acid (36 ml) under ice cooling, saturated brine (100 ml), and ethyl acetate (800 ml). The organic layer is taken, washed wi-th satura-ted brine, dried over sodium sulfate, and concentrated under reduced pressure. The residue is recrystallized from a tol-uene-ethyl aceta-te mixture to give (2S,4R-)-l t-butoxy-carbonyl-2-carboxy-4-hydroxypyrrolidine (84.7 g).
Yield: 96%. Colorless crystals. mp. 126 to 128~C.
NMR ~ (CDCl3) ppm: 1.43, 1.46(2 x s, 9H), 1.95 to 2.36(m, 2H), 3.36 to 3.6(m, 2~), 4.23 to 4.44(m, 2H).
IR ~ (CHCl3) cm l 3360, 1735, 1656.

Step D-2. Protection of a carboxyl group To a solution of (2S,4R)-l-t~butoxycarbonyl-2-carboxy-4-hydroxypyrrolidine (84.5 g: 0.365 mole) in dichloromethane (1.27 liter) in a nitrogen atmosphere at -30~C, triethy~amine (61.1 ml: 0.438 mole) and ethyl chloroformate (38.4 ml: 0.402 mole) are added, and the mixture is stirred for 40 minutes.
Step D-3. I'reparation of an 0-mesyl compound Th~ resulting reaction mixture containing (2S,4R)-1-t-butoxycarbonyl-2--ethoxycarbonyloxycarbonyl-4-hydroxypyrrolidine obtained in Step D-2 is cooled to -40~C, triethylamine (61.1 ml: 0.438 mole) and methane-sulfonyl chloride (31.1 ml: 0.402 mole) are added thereto, and the mixture is stirred for 40 minutes.

2~7~k3~
- 7~ -Step D-4. Reduction To the resulting react;ion mixture containing (2S,4R)-1-t-butoxycarbonyl-2-ethoxycarbonyloxycarbonyl 4-methanesulfonyloxypyrrolidineobtained in Step D-3 cooling at -40~C, tetra-n-butylammonium bromide (11.8 g: 0.0365 mole) and a solution of sodium borohy-dride (52.5 g: 1.35 mole) in water (55 ml) are added.
The mixture is allowed to warm ~o -10~C and stirred for 1 hour. The aqueous layer is acidi~ied with ~ilute hydrochloric acid to pH 3. The organic layer is taken, successively washed with aqueous sodium hydrogerl car-bonate and water, dried over magnesium sulfate, and concentrated under reduced pressure. The residue is recrystallized from a toluene-hexane mixture to give (2S,4R)-1-t-butoxycarbonyl-4-methallesulfonyloxypyrroli-dine-2-methanol (101.3 g). Yield: 94%. Colorless crys-tals. mp. 95 to 96~C.
NMR ~ (CDC13) ppm: 1.48(s, 9H), 1.78 to 2.02(m, lH), 2.3 to 2.48(m, lH), 3.05 (s, 3H), 3.5 to 3.65(m, 2H), 3.65 to 4.0(m, 2H), 4.03 to 4.25 (m, lH), 5.2(s, lH).
IR ~ (CHC13) cm~1: 3460, 1680.

Step D-5. Substitution for an acetylthio group A solui~ion of (2R,4S)-1-t-butoxycarbonyl-4-methanesulfonyloxypyrrolidine-2-methanol (i.e., a sub-strate) (11.8 g: 40 mmole) and potassium thioacetate (5.94 g: 52 mmole) in dimethylformamide (120 ml) is stirred at 65~C for 3.75 hours. The reaction rnixture is mixed with ethyl acetate (330 ml), ice water (100 ml), and lN-hydrochloric acid (20 ml) to adjust the aqueous layer at pH 4. The organic layer is taken, successively washed with water and saturated brine, dried over sodium sulfate, and concentrated under 2 V ~ 3 ~1 reduced pressure. The residue is purified by silica gel chromatography (toluene-ethyl acetate = 2:1) to give (2S,4S)-4-ace-tylthio-l-t butoxycarbonylpyrroli-dine-2-methanol (9.48 g). Yield: 86~. Pale orange colored oil.
NMR ~ (CDC13) ppm : 1.47(s, 9H), 2.3~(s, 3H), 2.4 to 3.2(m, 2H), 3.58 to 4.1(m, 6H).
IR ~ (CHCl3) cm~l: 3380, 1690.

Step D-6. In-troduction of a sulfamide group N-t-butoxycarbonylsul~amide is prepared in the same manner as in the paragraph (a) of Step A--6 in Preparative Example 7-A. To a solution of (2S,4S)-4-acetylthio~1-t-butoxycarbonylpyrrolidine~2-methanol (i.e., a substrate) (9.04 g: 32.~ mmole) in tetra-hydrofuran (THF') (95 ml), triphenylphosphine (PPh3) (10.16 g: 38.7 mmole), N-t-butoxycarbonylsulfamide (BSMD) (9.66 g: 49.2 mmole), and azodicarboxylic acid diethyl ester ~DE~D) (6.20 ml: 39.4 mmole) are succes-sively added under ice cooling. The conditions for this reaction are shown in Table 2, Step D-6. The reaction mixture is diluted with toluene (30 ml), concentrated, diluted with toluene (60 ml), and the formed crystals are filtered off. The filtrate is concentrated.

Step D-7. Removal of an acetyl group The residue obtained in Step D-6 is dissolved in toluene (95 ml), then, 4.92M sodium methoxide in methanol (20 ml: 98.4 mmole) is added at -35~C, and the mixture is stirred for 30 minutes. The reaction mix-ture is diluted with water (100 ml). The aqueous layer is ta~en, ethyl acetate (300 ml) is added, mixed with ~07~3~

- ~o -concentrated hydrochloric acid (10 m:L) under ice cool-ing, and the mi~ture is stirred. rrhe organic layer i8 taken, successively washed with water and brine, dried over sodium sulfate, and concentrated under reduced pressure. The residue is purified by silica gel chromatography. Obtained colorless oil is rcerystal-lized ~rom toluene-hexane mixture to give (2S,4S)-1-t-butoxycarbonyl-2-(N-t-butoxycarbonyl-N-sulfamoylamino~
methyl-4-mercaptopyrrolidine (9.32 g). Yield: 69%.
Colorless crystals. mp. 92 to 93~C.
NMR ~ (CDC13) ppm : 1.2 to 1.5(m, lH), 1.42(s, 9H), 1.54(s, 9H), 1.82 (d, J=6.2Hz, lH), 2.5 to 2.7(m, lH), 4.09, 3.05(A~X, J=12.0Hz, J=7.4}lz, J=8.2Hz, 2H), 4.06, 3.62(ABX, J=15.0Hz, J=10.8Hz, ~=3.2Hz, 2H), 4.2 to 4.6(m, lH), 6.08(s, 2H).
IR ~ (CHC13) cm 1 3380, 3220, 1718, 1680.
Elemental Analysi~ (C15H29N3O6S2) Calcd. : C, 43.78; H, 7.10; N, 10.21; S, 15.58 Found : C, 43.64; H, 7.10; N, 10.19; S, 15.34 .. ..

.

~07~;~3~
- 8:L -Table 1 KSAc DMF Temp. Time Yield Step equiv.a) vol.b) ~C min.

A-5 1.55 20 fold 70 90 75 B-5 1.20 5 fold 70 300 81.

C-7 1.30 10 fold 65 105 70 D-5 1.30 10 fold 65 225 86 a) Molar ratio to the substrate b) The volume (ml) of the solvent to the weight (g) of the substrate 2~76~3~J
- ~2 -Table 2 THF PPh3 BSMD l)EAD Temp. Time Yield Step vol.C) equiv.d) equiv.d) equiv.d) min. %

A-620 fold 1.34 1.20:L.30 45~C 150 76 B-67 fold 1.50 1.66 1.500~C 300 84 C-810 fold 1.28 1.501.30 room 240 82 -temp.

D-611 fold 1.18 1.501.20 room 180 __e) temp.

c) The volume (ml) of the solvent to the weight (g) of the substrate d) Molar ratio to the substrate e) Not measured :
' 2V76~3~
- ~3 -Table 3 NaOMe Solventg) Temp. Time Yield Stepequiv.f) vol.h) ~C min.

A-71.5 15 fold -40 120 72 8-72.0 5 fold -10 60 70 C-93.0 4 fold -35 30 85 D-73.0 11 fold -35 30 69 f) Molar ratio to the substrate Y) Dichloromethane is used in Steps A-7, B-7, C-9 and toluene is used in Step D-7.
h) The volume (ml) o~ the solvent to the weight (g) of the substrate 3 ~

Preparative Example 8 of a pyrrolidine derivative Ms~., 1 Ms~., 2 MsO., 3 H ~-~ ~ S02 ~ ~ ~ NFt - ' AlzAlz C6H4C1-p Alz A ~ 4 ~ 5~ ~
N ~ N~t ~N ~ NH2 ~ ~2NH2 Alz ~ Alz / Alz Step 1. Preparation of a p-chlorobenzenesul~onyl com-pound To a solution of (2S,4R)-1-allyloxycarbonyl-4-methanesulfonyloxypyrrolidine-2-methanol (13.4 g:
50 mmole) in dichloromethane (50 ml), p-chlolobenzenesulfonyl chloride (12.66 g: 60 mmole) is added in a nitrogen atmosphere at room temperature and a solution of triethylamine (8.69 ml: 62.5 mmole) in dichloromethane (10 ml) is further added dropwise. The mixtura is stirred a-t room temperature overnight. The reaction mixture is successively washed with aqueous sodium hydrogen carbonate and saturated brine, dried over magnesium sulfate, concentrated in vacuo, and purified by silica gel chromatography (toluene-ethyl acetate) to give crude (2S,4R)-1-allyloxycarbonyl-2-p-chlolobenzenesulfonyloxy-4-methanesul~onyloxypyrroli-dine (23.73 g) as oil. Yield: 105%.
NMR ~ (CDCl3) ppm: 2.2 to 2.6(m, 2H), 3.04(s, 3H), 3.58(dd, J=5.0Hz, 11.4Hz, lH), 3.8 to 4.0(m, lH), 4.1 2~7~

- ~5 -to 4.3(m, 3H), 4.5(m, 3H), 5.1 to 5.4(m, 3H), 5.7 to 6.0(m, lH).

Step 2. Preparation of a phthalimide compound To a solution of (2S,4R)-1-allyloxycarbonyl~
2-p-chlorobenzenesulfonyloxymethyl-4-methanesulfony-loxypyrrolidine (23.7 g: ca. 50 mmole) in dimethylfor~
mamide (50 ml), potassium phthalimide (10.2 g:
55 mmole) is added in a nitrogen a-tmoshpere, and the mixture is stirred at 60~C for 3.5 hours. The reaction mixture is poured into a stirring mis~ture of ice water (500ml) and ethyl acetate (500 ml). The organic layer is successively washed with water (4 times) and satu-rated brine, dried over magnesium sulfate, and concen-trated in vacuo. The resudue is recrystallized from a mixture of n-hexane and toluene. The solid is filtered off and the filtrate is purified by silica gel chroma-tography (toluene-ethyl acetate) to give crude (2S,4R)~
1-allyloxycarbo~yl-2-phthalimidomethyl-4-me-thanesulfo-nyloxypyrrolidine (12.41 g). Yield: 61%. Colorless oil.

Step 3. Preparation of an acetylthio compound A solution of (2S,4~)-1-allyloxycarbonyl-2-phthalimidomethyl-4-methanesulfonyloxypyrrolidine (12.4 g: 30.46 mmole) and 90~ potassium thioacetate (5.22 g: 45.69 mmole) in dimethylformamide (130 ml) is heated with stirring at 60~C for 4 hours. The reac-tion mixture is diluted with ethyl acetate (200 ml) and ice water (2~0 ml). The organic layer is taken, suc-cessively washed with water (3 times) and saturated brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel 2 ~ 3 ~1 - ~6 -chromatography -to ~ive crude (2S,4S)-4-acetylthio-l-allyloxycarbonyl-2-phthalimidomethylpyrrolidine (9.33 g). Yield: 81%.
NMR ~ ~CDCl3) ppm: 1.7 to 1.9(m, lH), 2.33(s, 3H), 2.4 to 2.7(m, lH), 3.25 (dd, J=6.8Hz, 11.4Hz, lH), 3.7 to 4.0(m, 2H), 4.0 ~o ~.2(m, 2H), 4.3 to 4.6(m, 3~), 5.0 to 5.3(m, 2H), 5.7 to 5.9(m, lH), 7.7(m, 2H), 7.85(m, 2H).

Step 4. Removal of a phthalyl and an acetyl groups To a solution of crude (2S,AS)-1-acetylthio-1-allyloxycarbonyl-2-phthalimidomethylpyrrblidine (5.61 g: 14.90 mmole) in dichloromethane (5.4 ml), and methanol (5.4 ml), hydrazine monohydrate (2.17 ml:
44.7 mmole) is added. The mixture is heated at 60~C
with stirring for 4 hours. The solid in the reaction mixture is filtered off, washed with dichloromethane (70 ml) and the washing is combined with the filtrate.
The mixture is concentrated to give crude (2S,4S)-2-aminomethyl-1-allyloxycarbonyl-4-mercaptopyrrolidine (2.80 g). Yield: 68~. Oil.

Step 5. Preparation of a sulfamoyl compound To a solution of (2S,4S)-2-aminomethyl-l-allyloxycarbonyl-4-mercaptopyrrolidine (2.80 g: ca.
13.14 mmole) in dichloromethane (66 ml) at -50~C, triethylamine (4.02 ml: 28.91 mmole) and trimethylchlo-rosilane (3.76 ml: 28.91 mmole) are added dropwise over 15 minutes. The mixture is stirred at the same teM-perature for 20 minutes. To the reaction mixture triethylamine (0.92 ml: 6.57 mmole) and a solution of sulfamoyl chloride (19.37 mmole) in dichloromethane (6.8 ml) are added dropwise over 20 minutes at -70~C, ~7~3~

and the mixture is stirred for 30 minutes. To the reac-tion rni~sture triethylamine (3.84 ml: 27.59 mmole) is again added over 1 hour at -50~C. The reaction mix-ture is kept at the same temperature overnight and concentrated in vacuo. The residual oil is purified by silica gel chromatography (ethyl acetate) to give (2S,4S)-l-allyloxycarbonyl-2-(sulfamoylamino)methyl-4-mercaptopyrrolidine (2.64 g). Yield: 68%. White powder.
NMR ~ (CDC13) ppm: 1.4 to 1.6(m, lH), 1.83(d, J=6.2Hz, lH), 2.5 to 2.7(m, lH), 3.11(dd, J=8.2Hz, 11.6Hz, lH), 3.3 to 3.4(m, lH), 3.71(dd, J=2.9Hz, 15.2Hz, lH), 4.13(dd, J=7.3Hz, J=11.7Hz, lH), 4.16(dd, J=10.3, 14.9Hz, lH), 4.3 to 4.6(m, 3H), 4.7(m, 2H), 5.2 to 5.4(m, 4H), 5.8 to 6.0(m, 2H), 6.0(m, 2H).
IR ~ (CHC13) cm l 684, 1158.

Preparative Example 9 of a pyrrolidine deriva-tive H0-., 1 Ms~......... 2 Ms~......... 3 ~NIlCOOMe ~COO~Ie ~OH
H HCl Alz Alz AcS~ ,. AcS~r~ Alz 5 H~ Alz NJ OH ' N~NS02NH2 N~NSC~2NH2 Alz Alz Alz Step 1. Preparation of an N-protected and an 0-mesyl compound To a suspension of (2S,4R)-4-hydroxy-2-methoxycarbonylpyrrolidine hydrochloride (17.0 g:

- ~3 -lO0 mmole ) in d:i chlox~ome thane (200 ml ), triethylamine (29.2 ml: 210 mmole ) ls added in a nitrogen a tmosphere under i.ce cooling. The mixture is stirred for 5 minutes at room temperature, mixed dropwise with a solution of ally:l chloro~ormate (11.2 ml: 100 mrnole) in dichloromethane (20 ml ), stirred for 1 hour at room tempera-ture, and dilu-t;ed with water (250 ml ) . The organic layer is taken, successively washed with wa ter and saturated brine, dried over magnesium sulfate, and concentrated in vacuo to give (2S,4R)-1-allyloxy-carbonyl-4-hydroxy-2-methoxycarbonylpyrrolidine (21.82 g ) as oil . To a solution o~ this product in dichloromethane ( 100 ml ), triethylamine (16.7 ml:
120 mmole ) and methanesulfonylchloride ( 9 .2 m~:
120 mmole ) are added in a nitrogen a tmosphere under ice cooling, and -the mixture is stlrred for 10 minute :; .
The reac tion mixture is successively washed with aque-ous sodium hydrogen carbonate and saturatad brine, dried over magnesium sulfate, concentrated in vacuo, and purified by silica gel chromatography ( toluene-ethyl acetate) to give (2S,4R)-1-allylo~-ycarbonyl-4-methanesulfonyloxy-2-methoxycarbonylpyrrolidine (27.62 g ) as oil . Yield: 90% .
NMR ~ (CDCl3) ppm: 2.2 to 2.4(m, lH), 2.2 to 2.5(m, lH), 2.5 to 2.8(m, 2H~, 3.06(s, 3H), 3.74 & 3.77(2 x s, 3H), 3.8 to 4.0(m, 2H), 4.4 to 4.7(m, 3H), 5.2 to 5.4(m, 3H), 5.8 to 6.0(m, lH).

Step 2. Preparation of a methylol compound To a solu-tion of (2S,4R)-1-allyloxycarbonyl-4-methanesul f onyloxy- 2 -methoxycarbonylpyrrolidine (27.12 g: 74.0 mmole ) ir a mixture of tetrahydrofuran (94 ml ) and e~hanol (14() ml ), sodium ~orohydride ~ 12 g:

~ ~ 7 ~ 3 31.7 mmole) is added in a nitrogen atmosphere under ice cooling. The mixture is stirred for 4 hours at room temperature. To the reaction mix-ture concentrated sulfuric acid (8.8 ml: 158.4 mrnole) is added dropw:ise under ice cooling. The reaction mixture is concentrat-ed to hal f a volume in vacuo, and diluted with ethyl acetate (100 ml) and ice water (100 ml). The organic layer is taken, sucoessively washed with aqueous sodium hydrogen carbonate and saturated brine, dried over magnesium sul~ate, and concentrated :Ln vacuo to give (2S,4R)-l-allyloxycarbonyl-4-methanesulfonyloxypyrroli-dine-2-methanol (19.33 y). Yield: 77 ~. Colorless oil.
NMR ~ (CDC13) ppm: 1.9 to 2.1(m, lH), 2.3 to 2.5(m, lH), 3.05(s, 3H), 3.5 to 3.7(m, 2~1), 3.7 to 4.1(m, 2H), 4.1 to 4.3(m, lH), ~.6(m, 2H), 5.2 to 5.4(m, 3H), 5.8 to 6.1(m, lH).

Step 3. Preparation of an acetylthio compound A solution of (2S,4R)-l-allyloxyc:rbonyl-4-methanesulfonyloxypyrrolidine-2-methanol (19.32 g:
69.17 mmole) and 90~ potassium thioacetate (10.73 g:
89.9 mmole) in dime~hylformamide (217 ml) is ~ea-ted with stirring at 65~C for 5 hours. To the reaction mixture ethyl acetate (200 ml) and ice water (200 ml) are added. The organic layer is taken, successively washed with aqueous 0.05N-sodium hydroxide, O.lN-hydro-chloric acid, water and satura-ted brine, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel chroma-tography to give (2S,4S)-4-acetylthio-1-allyloxycarbonylpyrroli-dine-2-methanol (15.34 g). Yield: 90~.

2~7~30 NMR ~ (CDCl3) ppm: 1.5 to 1.7(m, lH), 2.34(s, 3H), 2.4 to 2.6(m, lH), 3.19(dd, J=8.OHz, 11.5Hz, lH), 3.6 to 3.8(m, 2H), 3.8 to 4.0(rn, lH), 4.0 to 4.2(m, 2H), 4.6(m, 2H), 5.2 to 5.4(m, 2H), 5.B to 6.1(m, lH).

Step 4. Preparation of a sulfamide compound To a solution of (2S,4S)-4-acetylthio-1-allyloxycarbonylpyrrolidine-2-methanol (8.02 g: ca 30 mmole) in ethyl acetate (83 ml) under ice cooling, triphenylphosphine (9.44 g: 13.6 mmole), N-allyloxy-carbonylsulfamide (3.12 g: 15.9 mmole), and azodicar~
boxylic acid diethylester (5.67 ml: 36 mmole) are successively added. The mixture is stirred under ice cooling for 55 minutes and at room temperature ~or 4 hours. The reaction mixture is dissolved in toluene (60 ml), concentrated, diluted with toluene (60 ml), filtered to remove separating crystals, and the fil-trate is concentrated. The residue is puri~ied by silica gel chromatography to give (2S,4S)-4-acetylthio-1-allyloxycarbonyl-2-(N-sulfamoyl-N-allyloxycarbonyl-amino)methylpyrrolidine (6.74 g). Yield: 55%. Color-less oil.
NMR ~ (CDC13) ppm: 1.5 to 1.7(m, lH), 2.35(s, 3H), 2.5 to 2.7(m, lH), 3.19 (dd, J=6.3Hz, J=11.5Hz, lH), 3.68(dd, J=3.8Hz, J-14.5Hz, lH), 3.9 to 4.3(m, 3H), 4.3 to 4.7(m, 5H), 5.2 to 5.4(m, 4H), 5.8 to 6.1(m, 4H).

Step 5. Removal of an acetyl group To a solution of (2S,4S)-4-acetylthio-1-allyloxycarbonyl-2-(N-sulfamoyl-N-allyloxycarbonylami-no)methylpyrrolidine (6.70 g: 16.4 mmole)in -toluene (50 ml), 4.92 M solution o~ sodium methoxide in metha-~6!~3 nol (5.0 ml: 24.7 mrnole) is added at -30~C. The mix-cure is stirred for 30 minutes, and diluted with water (55 ml). The aqueous layer is -taken, diluted with -toluene (50 ml), acidified with concentrated hydrochloric acid (2.3 ml) under ice cooling, and stirred. The organic layer is taken, successively washed with water and saturated brine, dried over magnesium sulfa-te and concentra-ted in vacuo. The residual oil is purified by silica gel chromatography (toluene~e-thyl acetate) to give (2S,4S)~1-allyloxycar-bonyl-2-(N-sulfamoyl-N-allyloxycarbonylamino)methyl-4-mercaptopyrroli.dine (4.89 g). Yield: 78~. Colorlessoil.
NMR ~ (CDC13) ppm: 1.5 -to 1.7(m, lH), 2.35(s, 3H), 2.5 to 2.7(m, lH), 3.19(dd, J=6.3Hz, J=11.5Hz, lH), 3.68(dd, J=3.8Hz, J=14.5Hz, lH), 3.9 -to 4.3(m, 3H), 4.3 to 4.7(m, 5H), 5.2 -to 5.4(m, 4H), 5.8 to 6.1(m, 4H).
IR ~ (CHCl3) cm~1: 1718, 1684, 1179, 1160.

Examples OXl Me OXlMe R4 NS02N~R3 ~ox2 '~'' coox2 e c .,: -, . . .

207 :;~3~

Example Xl X2 y2 -Nso2N~R3 1 H PMB Boc -Nt~S02NH2 2 H PNB Pnz NUS02~H2 3 H PMB Pffle -NHS02N~H

4 H PMB Pmz ~NtLgo2N~mez H PMB Pmz -NHS02N'Me 6 SiMe3 CMPh2 Boc _N,Boc 7 H PMB Pa~ -NHS02NHCH2CH20H
8 H PMB Pmz -N ~ NFmz 9 H PMB Pmz -N~c,NPme ~7~3~3 OH Me R4 ~ ~Nso2N~R3 2 (~ lH
COOH

Example - NS02N~R3 3 -~HSO2NH2 4 ~ 502NH~Ie -NHS02N~e 7 -NHSO2NHCHzCH20H
.
8 -~N~Noll 9 -~N~oH

.

~r~

_ 9~ _ Example 1. Synthesis of a (3S,5S)-pyrrolidylthio-carbapenem derivative Step 1. Preparation of a pro-tected pyrrolidylthio-carbapenem dexivative To a solu-tion of (1R,5S,6S)-6-[(lR)-1-hydroxyethyl]-2-oxo-1-methyl-1-carbapenam-3-carboxylic acid p-methoxybenzyles-ter (1.45 g) in acetonitrile (15 ml) at -25~C, diphenylpho~phoric acid chloride (0.953 ml) and diisopropylethylamine (0.872 ml) are successively added. The ml~ture is stirred at room temperature for 1 hour. To this nix-ture 2-sulfamoyl-aminomethyl-l-t-butoxycarbonyl-4-mercaptopyrrolidine (1.69 g) and diisopropylethylamine (0.945 ml) are added under ice cooling, and the mixture is stirred for 22 hours. The reaction mixture is mixed with lN-hydro-chloric acid (15 ml) and diluted wi-th ethyl ace-tate.
The organic layer is taken, washed with water, dried over sodium sulfate and concentrated. ~he residue is purified by column chromatography over 10~ wet silica gel to give (lR,5S,6S)-6-[(lR)-1-hydroxyethyl]-2-[(3S,5S)-5-sulfamoylaminomethyl-l-t-butoxycarbonylpyr-rolidin-3-yl]thio- 1-methyl-1-carba-2-penem-3-carboxyl-ic acid p-methoxybenzylester (1.61 g). Yield: 60%.
Pale yellow foam.
NMR ~ (CDCl3) ppm: 1.25(d, J=7.2Hz, 3H), 1.32(d, J=6.4Hz, 3H), 1.47(s, 9H), 1.75 to 2.0(m, lH), 2.4 to 2.65(m, lH), 2.61(br s, 4H), 3.1 to 3.7 (m, 6H), 3.81(s, 3H), 3.75 -to 4.25(m, 4H), 5.19, 5.25(ABq, J=12.1Hz, 2H), 6.89(d, J=8.6Hz, 2H), 7. 39(d, J=8.6Hz, 2H).
IR ~ (CHCl3) cm 1 3400, 3290, 1770, 1682.

2 0 ~

Step 2. Deprotection To a solution of (:LR,5$,6S)-6-[(lR)-1-hydroxyethyl]-Z-[(3S,5S)-5-sulEamoylaminomethyl-1-t-butoxycarbonylpyrrolidin-3-yl]thio-l-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzylester (1.083 g) in a mixture of dich]oromethane (16.5 ml), anisole (1.52 ml) and nitromethane (3.1 ml) at -60~C, a solu-tion of l.OM aluminum chloride in nitromethane (12.93 ml) ~s added dropwise. The mixture is stirred for 2 lQ hours slowly warming up to -40~C. The reaction rnixture is poured into a solution of sodium acetate (3.18 g) in water (24 ml), successively washed with ether and ether-petroleum ether, desalted and purified by sty-rene-divinylbenzene copolymer resin column chromatogra-phy, and lyophilized the objective fraction to ~ive (lR,5S,6S)-6-[(lR)-l-hydroxyethyl]-2-[(3S,5S) 5-sulfamoylaminomethyl-1-pyrrolidin-3-yl]thio-1-methyl-l-carba-2-penem-3-carboxylic acid (0.429 g). Yie;~ld: 67%.
Pale yellow ~oam.
NMR ~ (D20) ppm: 1.22(d, J=7.2Hz, 3H), 1.27(d, J=6.3Hz, 3H), 1.64 to 1.82(m, lH), 2.62 to 2.80(m, lH), 3.26 to 3.59(m, 5H), 3.63 to 3.76(m, lH), 3.84 to 4.;0 (m, 2H), 4.16 to 4.29(m, 2H).
IR ~ (KBr) cm 1 3400, 1750.
MIC ( ~ / ml) : Staphylococcus aureus strain 3626:
25, Streptococcus pyogenes C203: <0.003.

Example 2. Coupling 2 of a (3S,5S)-pyrrolidylthio-carbapenem derivative Step 1. Preparation of a Protected pyrrolidylthio-carbapenem derivative To a solution of (lR,5S,6S)-6-[(lR)-l-hydroxyethyl]-2-diphenoxyphosphonyloxy-l-methyl-1-~7~3~J

carba-2-penenn-3-carboxylic acid p-nitrobenzylester (3.0~ g: 5.12 mmole) in acetoni-trile (30 ml) under ice cooling, a solution of diisopropylethylamine (1.16 ml:
1.3 eq.) and 2-sul~amoylaminometyl-1-p-ni-trobenzyloxy-carbonyl-4-mercaptopyrrolidine (2.4 g: 1.2 eq.) in acetonitrile (20 ml) is added. The mixture is stirred under ice cooling for 140 minutes. The reaction mix-ture is diluted with ethyl acetate, successively washed wi-th water and saturated brine, dried over magneslum sulfate and concentrated. The residue is purified by silica gel column chromatography (toluene : ethyl aceta-te) to give (lR,5S,6S)-6-[(lR)-l-hydroxyethyl~-2-[(3S,5S)-5-sulfamoylaminomethyl-1-p-nitrobenzyloxy-carbonylpyrrolidin-3-yl~-thio-1-methyl-1-carba-2-penem-3-carboxylic acid p-nitrobenzylester (3.35 g).
Yield: 89~.
NMR ~ (CDC13) ppm: 1.2~(d, J=7~1z, 3H), 1.37(d, J=6Hz, 3H), 4.68(s, 2il), 5.22, 5.50(ABq, J=14Hz, 2H), 5.23(s, 2~1), 7.52(d, J=9Hz, 2~), 7.65 (d, J=9Hz, 2H), 8.21(d, 2.0 J=2.5Hz, 2H), 8.26(d, J=2.5Hz, 2H).
IR ~ (CHC13) cm~l: 1773, 1720, 1704.

Step 2. Deprotection To a solution of (lP~,5S,6S)-6-[(lR)-l-hydroxyethyl]-2-[(3S,5S)-5-sulfamoylaminomethy~-1-p-nitrobenzyloxycarbonylpyrrolidin-3-yl]thio-1-methyl-1-carba-2-penern-3-carboxylic acid p-nitrobenzylester (3 g) in a mixture of tetrahydrofuran (60 ml) and O.lM-MES buffer (pH 7.0), 1~'~ palladiurn on carbon (2 g) as a catalys-t is added. The mixture is shaken under a stream of hydrogen at atmospheric pressure for 4 hours.
The reaction mix-ture is filtered to remove the cata-lyst, washed with ethyl acctate to remove a neutral 2 ~ 7 ~

substance, and concentrated. The residual aqueous solution is purified by s-tyrene-divinylbenzene copolymer resin column chromatography. The fraction eluting wi-th 5 to 10% ethanol water is lyophilized to give (lR,5S,6S)-6-[(lR)-l-hydroxyethyl]-2-[(3S,5S)-5-sulfamoylaminomethyl~l-pyrrolidin-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid (1.42 g). Yield:
84.8~.

Example 3. Synthesis of a (3S,5S)-pyrrolidylthio-carbapenem derivative Step 1. Preparation of a protec-ted pyrrolidylthio-carbapenem berivative To a solution of (lR,5S,6S)-6-[(lR)-l-hydroxyethyl]-2-dipheno~syphosphonyloxy-1-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzylester (1 mmole) in acetonitrile (lO ml) under ice cooling, diisopropylethylamine (1.2 mmole) and 2-p-methoxybenzy-loxycarbonylsulfamoylaminomethyl-l-p-methoxybenzyloxy-carbonyl-4-mercaptopyrrolidine (1 mmole) are added.
The mixture is allowed to stand overnight. The reaction mixture is diluted with dichloromethane, successively washed with dilute hydrochloric acid, water, aqueous sodium hydrogen carbonate, and brine, dried and concentrated. The residue is purified by silica gel column chromatography to give (lR,5S,6S)-6-[(lR)-l-hydroxyethyl]-2-[(3S,5S)-5-p-methoxybenzyloxycarbonylsulfamoylaminomethyl-l-p-met-hoxybenzyloxy-carbonylpyrrolidin-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzylester.
Yield : 50 to 80~.
NMR ~ (CDCl3) ppm: 1.20(d, J=6.4Hz, 3H), 1.34(d, J=6.1Hz, 3H), 3.7S(s,9H), 5.00 to 5.12(m, 4H), 5.23, ~07~3~

- 9~

5.15(ABq, J=14.0~lz, 2~1).
IR ~ (CHCl3) cm 1 3390, 1770, 1740, 1693, 1610.

Step 2. Deprotection To a solution o~ (lR,5S,6S)-6-[(lR)-1-hydroxyethyl]-2-[(3S,5S)-5wp-methoxybenzyloxycarbonyl-sulfamoylaminomethyl-1-p-methoxybenzyloxycarbonyl-pyrrolidin-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzylester (1 mmole) in dichloromethane (2.0 ml) at -40~C, anisole (10 mmole) and a solution o~ 2M aluminum chloride in nitromethane (3 to 4 ml) are added. The mixture is stirred at the same temperature for 1 to 1.5 hours. The reac-tion mixture is poured into a solution of sodium acetate (19 to 25 mmole) in water (100 ml), washed with dichlo-romethane to remove a neutral substance. The aqueous layer is puri~ied by styrene-divinylbenzene copolymer resin column chromatography. The objective eluate is lyophlized to give (1~,5S,6S)-6-[(lR)-l~hydroxyethyl]-2-~(3S,5S)-5-sulfamoylaminomethyl-1-pyrrolidin-3-yl~-thio-1-methyl-1-carba-2-penem-3-carboxylic acid.
Yield: 60 to 70%.

Example 4. Synthesis of a (3S,5S)-pyrrolidylthio-carbapenem derivative Step 1. Preparation of a protected pyrrolidylthio-carbapenem derivative To a solution of (lR,5S,6S)-6-[~lR)-l-hydroxyethyl]-2-diphenoxyphosphonyloxy-1-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzylester (700 mg) in acetonitrile (4 ml) at _30 a C, a solution of diisopropylethylamine (182 ,ul) and 1-p-methoxyben7.y-loxycarbonyl-4-mercapto-2-(N-p-methoxybenzyloxycarbo-~ ~ 7 ~
_ ~9 _ nyl-N-methylaminosulfonylaminomethyl)pyrrolidine (~01 mg) in acetoni-trile (3 ml) is added. The rnixture is stirred under ice cooling ~or 90 minutes. The reaction mixture is poured into a mixture of ethyl acetate and dilute hydrochloric acid. The ethyl ace-tate layer is taken, successively ~ashed with water, aqueous sodium hydrogen carbona-te, an~ brine, dried over magnesium sulfate, and concentrated in vacuo. rrhe residue is purified by silica gel column chromatography (toluene : ethyl ace-tate Y 1 : 2) to give (lR,5S,6S)-6-[(lR)-1-hydroxyethyl]~2-[(3S,5S)-l-p-meth-oxybenzyl-oxycarbonyl-5-(N-p-methoxybenzyloxycarbo- nyl-N-methyl-aminosulfonylaminomethyl)pyrrolidin-3-yl]thio-1-meth-yl-1-carba-2-penem~3-carboxylic acid p-methoxybenzyl-ester (512 mg).
NMR ~ (CDCl3) ppm: 1.22(d, J-7.0Hz, 3H), 1.34(d, J=6.4Hz, 3H), 1.6 to l.9(m, lH), 2.25 to 2.5(m, lH), 3 to 3.6(m, 7H), 3.778(s, 3H), 3.783(s, 3H), 3.738(s, 3H), 5.05(s, 2H), 5.13(s, 2H), 5.2(ABq, J=12Hz, 2H), 6.3 to 6.5(m, lH), 6.8 to 7.0( m, 6H), 7.2 to 7.4(m, 6H).
IR ~ (C~C13) cm~1: 1767, 1697.

Step 2. Deprotection To a solution of (lR,5S,6S)-6-[(lR)-l-hydroxyethyl]-2-[(3S,5S)-1-p-metho~ybenzyloxycarbonyl-5-(N-p-methoxybenzyloxycarbonyl-N-methylaminosulfonyl-aminomethyl)pyrrolidin-3-yl]thio-1-methyl 1-carha-2-penem-3-carboxylic acid p-methoxybenzylester (610 mg) in a mix-ture of dichloromethane (6 ml), nitromethane (2 ml) and anisole (4 ml) stirring at -~0~C, 2M-solu-tion of aluminum chloride in nitromethane (2.6 ml:
7.5 equivalents) is added. The mixture is stirred at ~7S~
~- 100 --35 ~5~C for 1 hour and 30 mlnutes. The reaction mix-ture is poured into a mixture of sodium aoetate(l.34 g), water (20 ml) and dichloromethane (20 ml). The aqueous layer is taken, subjected to a styrene-divinylbenzene copolymer resin column chroma-tography, and the ~raction eluting with 8~ ethanol is lyophilized to give (lR,5S,6S)-6-[(lR)-1-hydroxyeth--yl]-2-[(3S,5S)-5-N-methylaminosulfonylamino-methyl-pyrrolidin-3-yl]thio-l-methyl-1-carba-2-penem-3-carbox-10ylic acid (206 mg). Yield : 68.6%.
NMR ~ (CDCl3) ppm: 1.22(d, J-7.0Hz, 3H), 1.27(d, J=6.~Hz, 3H), 1.5 to 1.8(m, lH), 2.63(s, 3~), 2.6 to 2.~(m, lH), 3.1 to 3.6(m, 5~), 3.65, 3.72(dd, ~=6.6Hz, J=7.6Hz, lH), 3.8 to 4.4(m, ~H).
15IR ~ (CHCl3) cm ~: 1750, 1585.
MIC ( ~ / ml) : Staphylococcus aureus strain 3626:
25, S-treptococcus pyogenes C203: ~0.003.

Example 5. Synthesis of a (3S,5S)-pyrrolidyithio-carbapenem derivative Step 1. Preparation of a protec-ted pyrrolidylthio-carbapenem derivative To a solution of (lR,5S,6S)-6-[(lR)-l-hydroxyethyl]-2 diphenoxyphosphonyloxy-l-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzylester (l mmole) in acetonitrile (10 ml) under ice cooling, di-isopropylethylamine (1.2 mmole) and 2-N,N-dimethyl-sulfamoylaminomethyl-l-p-methoxyben~yloxycarbonyl-~-mercaptopyrrolidine (1 mmole) are added. The mlxture is allowed to stand overnight. The reaction mixture is diluted with dichloromethane, successively washed with dilute hydrochloric acia and water, dried, and concen-trated. The residue is purified by silica gel column .. .

chromatography -to give (lR,5S,6S)-6-[(lR)-1-hydroxyeth-yl~-2-[(3S,5S)-5-N,N-dimethylsulEamoylaminomethyl-1-p-methoxybenzyloxycarbonylpyrrolid:in-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzylester, Yield: 50 to 80%.
NMR ~ (CDC13) ppm: 1.22(d, J=7.2Hz, 3H), 1.34(d, J=6.2Hz, 3H), 2.76(S, 6H), 3.79(s, 3H), 3.81(s, 3H), 5.06(s, 2H), 5.24, 5.18(~Bq, ~=12Hz, 2H).
IR ~ (C~IC13) cm~1: 3390, 1770, 1725, 1690, 1610.
Step 2. Deprotection A solution of (lR,5S,6S)-6-[(lR)-1-hydroxy-ethyl]-2-[(3S,5S)-5-N,N-dimethylsul~amoylaminomethyl-1-p-methoxybenzyloxycarbonylpyrrolidin-3-yl]thio-1-15 methyl-1-carba-2-penem-3-carboxylic acid p-methoxy-benzylester (1 mmole) in dichloromethane (20 ml) is cooled to -40~C. Anisole (10 mmole) and a solution of 2M aluminum chloride in nitromethane (3 to 4 ml) are added -thereto, and the mixture is stirred at the same 20 temperature for 1 to 1.5 hours. The reaction mixture is poured into a solution of sodium acetate (19 to 25 mmole) in water (100 ml), and washed with dichloro-methane to remove a neutral material. The aqueous layer is purified by styrene-divinylbenzene copolymer 25 resin column chromatography and the objective eluate is lyophilized to give (lR,5S,6S)-6-[(lR)-1-hydroxyethyl~-2-[(3S,5S)-5-N,N-dimethylsulfamoylaminomethylpyrro-lidin-3-yl]~hio-1-methyl-1-carba-2-penem-3-carboxylic acid. Yield: 60 to 70~.
NMR ~ (D20) ppm: 1.2(d, Ja7.4Hz, 3H), 1.28(d, J=6.4Hz, 3H), 1.65 to 1.80(m, lH), 2.65 to 2.80(m, lH), 2.81(s, 6H), 3.29 to 3.55(m, 5H), 3.65 to 3.75(m, lH), 3.80 to 4.10(m, 2H), 4.16 to 4.30(m, 2H).

207~3~

IR ~ (KBr) cm 1; 3~00, 1750.
MIC ( ~ / ml) : Staphylococcus aureus strain 3~26:
25, Streptococcus pyogenes C203: <0.003.

Example 6. Syn-thesis o~ a (3S,5S)-pyrrolidylthio-carbapenem derivative Step 1. Preparation of a protected pyrrolidylthio-carbapenem derivative To a solution of (lR,5S,6S)-2-diphenoxy-phosphonyloxy-6-[(lR)-l-hydroxyethyl]-1-methyl-J.-carba-2-penem-3-carboxylic acid diphenylmethylester (6.88 g:
11 mmole) in dichloromethane (70 ml) under ice cool.ing, trimethylchlorosilane (1.81 ml: 14.3 mmole) and triethylamine (1.99 ml: 14.3 mrnole) are added. The mixture is stirred for 25 minutes. The reaction mix-ture is poured into aqueous sodium hydrogen carbonate.
The organic layer is taken, washed with water and brine, dried over sodium sul~ate, and concentrated under reduced pressure. ~he residue containing the product, (lR,5S,6S) 2-diphenoxyphosphonyloxy-1-methyl-6-[(lR)-1-trimethylsilyloxyethyl]-1-carba-2-penem 3-carboxylic acid diphenylmethylester is dissolved in acetonitrile (70 ml), and (2S,4S)-1-t-butoxycarbonyl-2-(N-t-butoxycarbonyl-N-sul~amoylamino)methyl-4-mercap--topyrrolidine (5.g3 g: 13.2 mmole) and diisopropylethy-lamine (2.30 g: 13.2 mmole) are added thereto under ice cooling. The obtained mixture is stirred for 4.5 hours. To the reaction mixture containing the product, (lR,5S,6S)-2-[(3S,5S)-1-t-butoxycarbonyl-5-(N-t-butoxycarbonyl-N-sulfamoylamino)methylpyrrolidin-3-yl]thio-l--methyl-6-[(lR)-1-trimethylsilyloxyethyl]-1-carba-2-penem-3-carboxylic acid diphenylmethylester, lN-hydrochloric acid (5.5 ml) is added, and the mixture ~7~3~

is stirred for 20 minutes, diluted with ethyl acetate(150 ml), and the mix-ture is poured into ice water. The organic layer is taken, successively washed wi-th aqueous sodium hydrogen carbona-te, water, and brine, dried over sodium sulfate, and concentrated under reduced pressure. The residue is recrystallized from toluene to give (lR,5S,6S)-2-[(3S,5S)-1-t-butoxy-carbonyl-5-(N-t-butoxycarbonyl-N-sulfamoylamino)methyl-pyrrolidin-3-yl]-thio-6-[(lR)-l-hydroxyethyl]-l-methyl-1-carba-2-penem-3-carboxylic acid diphenylmethylester (7.53 g). Yield: 87~. Colorless crystals. mp.163 to 164~C.
NMR ~ (CDC13) ppm : 1.27(d, J=7.2Hz, 3H), 1.39(s, 9H), 1.42(s, 9H), 2.45 to 2.65(m, lH), 3.1 to 3.35(m, 2H), 3.28(dd, J=7.2Hz, J=2.6Hz, lH),3.5 to 3.77(m, 2H), 3.9 to 4.15(m, 2H), 4.26(dd, J=7.0H~, ~=2.6Hz, lH), 4.2 to 4.37(m, lH), 4.45 to 4.66(m, lH), 6.07(s, 2H), 6.95(s, lH), 7.2 to 7.6(m, lOH).
I~ ~ (CHC13) cm~l: 3385, 3230, 1778, 1715, 1685.
Elemental AnalysiS (C38H50N4010S2) Calcd.: C, 57.99; H, 6.40; N, 7.12; S, 8.15.
Found : C, 57.87; H, 6.46; N, 6.99; S, 7.93.

Step 2. Deprotection - To a solution of aluminum chloride (3.20 g:
24 mmole) in a mixture of anisole (24 ml) and dichloro-methane (24 ml) at -40~C, a solution of (lR,5S,6S)-2-[(3S,5S)-l-t-butoxycarbonyl-5-(N-t-butoxy_arbonyl-N-sulfamoyl-amino)methylpyrrolidin-3-yl~thio-6-[(lR)-l-hydroxy-ethyl]--1-methyl-1-carba-2-penem 3-carboxylic acid diphenylmethylester (2.36 ~: 3 mmole) in dichloro-methane (12 ml) is dropwise and gradually added. The mix-ture is vigorously stirred at -25 to -30~C for ~7~

- 10'1 -3.5 hours. The reaction mixture is poured into a solution of sodium acetate (5.91 g: 72mmole) in water (48 ml). The a~ueous layer is taken, washed with dichloromethane, concentrated in vacuo to remove re-maining orgaic solvent and subjected to styrene-divi-nylbenzene copolymer resin coumn chromatography. The fraction elu-ting with rnethanol-water (1:9) is lyophil-lized to give (lR,5S,6S)-6-[~lR)-1-hydroxyethyl]-2-[(3S,5S)-5-sulfamidomethylpyrrolidin-3-yl~thio-1-meth-yl-1-carba-2-penem-3-carboxylic acid (910 mg).
Yield: 72~. Colorless foam.
NMR ~ (D20) ppm: 1.22(d, J=7.2Hz, 3H), 1.27(d, J=6.3Hz, 3H), 1.64 to 1.82(m, lH), 2.62 to 2.80(m, lH), 3.26 to 3.59(m, 5H), 3.63 to 3.76(m, lH), 3.84 to 4.10(m, 2H), 4.16 to 4.29(m, 2H).
IR ~ (KBr) cm 1 3400, 1750.
MIC ( ~ / ml) : Staphylococcus aureus 3626: 25.
Blood level: mice i.v., after 15 min. ( ~ / ml): 9.8.
Urinary recovery: mice i.v., (~): 36.3.
Example 7. Synthesis of a (3S,5S)-pyrrolidylthio-carbapenem derîvative Step 1. Preparation of a protected pyrrolidylthio-carbapeneln derivative To a solution of (lR,5S,6S)-6-[(lR)~l-hydroxyethyl]-1-methyl-2-oxo-1-carbapenam-3-carboxylic acid p-methoxybenzylester (277 mg) in acetonitrile (4 ml) under ice cooling, diphenylphosphoric acid chloride (198 ~l) and diisopropylethylamine (166 ,ul) are successively added. The mixture is stirred at room temperature for 1 hour. To the reaction mixture con-taining the product, (lR,5S,6S)-2-diphenoxyphosphorly-loxy-6-[(lR)-1-hydroxyethyl]-l-methyl-l-carba-2-penem-., ' . ' .

~ o ~

3-carboxylic acid p-methoxybenzylester, (2S,4S)-2-(2-hydroxyethyl)sulfamoylaminome-thyl-1-p-methox~benzy-loxy-carbonyl-4-mercaptopyrrolidine (344 mg) and diisopropylethylamine (166 ,ul) are added under ice cooling, and the mixture is stirred at the same temper-ature for 2 hours. The reaction mixture is diluted with ethyl acetate, successively washed with water, dilute hydrochloric acid, water, aqueous sodium hydro-gen carbonate and water, dried over magnesium sulfate, and concentrated. The residue is purified by sillca gel column chromatography to give (lR,SS,6S)-6-C(lR)-l-hydroxyethyl]-2-C(3S,5S)-5-(2-hydroxyethyl)sulfamoyl-aminomethyl-l-p-methoxybenzyloxycarbonylpyrrolidin-3-yl]thio-l~methyl-l-carba-2~penern-3-carboxylic acid p-methoxybenzylester (156 mg). Yield: 26%.
NMR ~ (CDC13) ppm: 1.22(d, ~=7.0Hz, 3H), 1.34(d, J=6.2Hz, 3H), 3.79(s, 3~), 3.80(s, 3H), 5.05(s, 2H), 5.17, 5.24(ABq, J=12.2Hz, 2H).
IR ~ (CHC13) cm~l: 1775, 1690.
Step 2. Deprotection To a solution of (lR,5S,6S)-~-[(lR)-1-hydroxyethyl]-2-[(3S,5S)-5-(2-hydroxyethyl)sulfamoyl-aminomethyl-1-p-methoxybenzyloxycarbonylpyrrolidin-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzyl ester (148 mg) in a mixture of dichlo-romethane (3 ml) and nitromethane (750 ,ul) in a nitro-gen atmosphere at -40~C, a solution of 1. OM aluminum chloride in nitromethane (1.8 ml) and anisole (258 ,ul) is added. The mixture is stirred at the same tempera-ture ~or 1.5 hours. The reaction mixture is poured in-to a solution of sodium acetate (454 mg) in water (8 ml) and washed with an ether-hixane mixture. The 2~7~v~3~

aqueous laye~ is concentratecl :In vacuo to 4 ml, an~
purified by styrene-divinylberlr~ene copolymer resin column chromatography to give (lR,5S,6S)-6-[(lR)-1-hydroxyethyl]-2-[(3S,5S)-5-(2-hydroxyethyl)sulfamoyl-aminometh~lpyrrolidin-3-yl]~hio-1-me-thyl-1-carba-2-penem-3-carboxylic acid (42 mg). Yield: 46%.
NMR ~ (D20) ppm: 1.21(d, J=7.4Hz, 3H), 1.28(d, J=6.4Hz, 3H), 1.66 to 1.81(m, lH), 2.66 to 2.81(m, lH), 3.15(t, J=5.6Hz, 2H), 3.32 to 3.54(m, 5H), 3.65 to 3.75(m, 3~1), 3.87 to 4.07(m, 2H), 4.18 to 4.27(m, 2H).
IR ~ (KBr) cm ~: 3400, 1750.
Blood level: mice i.v., after 15 min (~ / ml): 29.3.

Example 8. Synthesis of a (3S,5S)-pyrrolidylthiocar-bapenem derivative Step 1. Preparation of a protected pyrrolidylthiocar--bapenem derivative To a solution of (lR,5S,6S)-2-diphenoxy-phosphonyloxy-6-[(lR)-l-hydroxyethyl]-l-methyl-l-carba 2-penem-3-carboxylic acid p-methoxybenzylester (~56 mg) in acetonitrile (3 ml) under ice cooling, diisopropy-lethylamine (165 ul) and (2S,4S)-2-(1,1-dioxo-2-p-methoxybenzyloxycarbonyl-1,2,5-thiadiazolidin-5-yl) methyl-~-mercapto-l-p-methoxybenzyloxycarbonylpyrr lidine (445 mg) are added. The mixture is allowed to stand at O~C oTiernigh~. The reaction mixture is dilut-ed with ethyl acetate, successively washed with water, dilute hydrochloric acid and water, dried over magnesi-um sulfate, and concentrated in vacuo. The residue ispurified by silica gel chromatography to give (lR,5S,6S)-2-[(3S,5S)-5-(1,1-dioxo-2-p-methoxybenzyl-oxycarbonyl-1,2,5-thiadiazolidin-5-yl)-methyl-l-p-meth-2 ~ 3 ~

oxybenzyloxycarbonylpyrrolidin-3~yl]-thi.o~6~[(1R)-1~
hydroxyethyl]-1-methyl~l-carba~2-penem~3--carboxylic acid p-methoxybenzylester (510 mg). Yield- 72%.
NMR ~ ( CDC13) ppm: 1.22(d, J=7.4Hz, 3H), 1.34(d, J=6.2Hz, 3H), 5.04(s, 2H), 5.23(s, 2H), 5.18, 5.24(AB~, -J=11.9Hz, 2H).
IR ~ (CHC13) cm 1 1773, 1735, 1700.

Step 2 Deprotec-tion To a solu-tion o~ ,5S,6S)-2-[(3S,5S)-5~
(1,1-dioxo-2-p-methoxybenzyloxycarbonyl-1,2,5~thia-di-azolidin-5~yl)methyl-1-p-methoxybenzyloxycarbonyl-pyrrolidin-3-yl]thio-6-[(lK)-l-hydroxyethyl]-1-methyl 1-carba-2~penem-3-carboxylic acid p-methoxybenzylester (500 mg) in a mixture of dichlorome-thane (8 ml) and nitrome-thane (3 ml) in a nitrogen atmosphere at -40~C, anisole (729 ~1) and a solution (5.03 ml) of 1. OM
aluminum chloride in nitromethane are added. The mix-ture is stirred at the same temperature for 1.5 hours.
2Q The reaction mixture i5 poured into a soution o~ sodium acetate (1.28 g) in water (50 ml), then aqueous layer is taken, and washed with an ether-hexane mixture. The aqueous layer is concentrated under reduced pressure to about 15 ml, and is purified by styrene-divinylbenzene copolymer resin column chromatography to give (lR,5S,6S)-2-[(3S,5S)-5~(1,1-dioxo-1,2,5-thiadiazoli-din-2-yl)methylpyrrolidin-3-yl]thio-6-[(lR)-1-hydroxy-ethyl]-1-methyl- 1-carba-2-penem-3-carboxylic acid (180 mg). Yield: 72%.
NMR ~ (D20) ppm~ 1.21(d, J=7.4Hz, 3H), 1.28(d, J=6.4Hz, 3H), 1.68 to 1.84(m, lH), 2.71 to 2.85(m, lH), 3.28 to 3.77(m, lOH), 3.94 to ~.12( m, 2H), 4.17 to 4.31(m, 2H).

2 ~ 3 0 - :L08 -IR ~ (KBr) cm~1: 3400, 1750.
MIC ( ~ /ml): Staphylococcus aureus strain 3626: 25.
Blood level: mice i.v., after 15 min ( ~ / ml): 31.8.

Example 9 Synthesis of a (3S,5S)~pyrrolidylthiocarbape-nem derivative Step 1. Preparation of a protected pyrrolid~lthiocar-bapenem derivative To a solution of (lR,5S,6S)-2-diphenoxy-phosphonyloxy-6-~(lR)-l-hydroxyethyl]-1-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzyl ester (638 mg) in acetonitrile (6 ml) under lce cooling, diisopropylethylamine (230 ~1) and (2S,4S)-2-(1,1-dioxo 2-p-methoxybenzyloxycarbonyl-3,4,5,6-te~rahydro-1,2,6-thiadiazin-6-yl)methyl-4-mercapto-1-p-methoxyben-zyloxycarbonylpyrrolidine (700 mg) are added. The mixture is stirred at 5~C for 2 hours and at room temperature for 1 hour. The reaction mixture is dilut-ed with ethyl aoetate, successively washed with water, dilute hydrochloric acid, water, aqueous sodium hydro-gen carbonate and water, dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by silica gel chromatography -to give (lR,5S,6S)-2-[(3S,5S)-5-(1,1-dioxo-2-p-methoxybenzyloxycarbonyl-3,4,5,6-tetrahydro-1,2,6-thiadiazin-6-yl)methyl-1-p-methoxybenzyloxycarbonylpyrrolidin-3-yl]thio-6-[(lR)-1-hydroxyethyl]-1-methyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzyl ester (640 mg). Yield: 64%.
NMR ~ (CDC13) ppm: 1.22(d, J=7.4Hz, 3H), 1.34(d, J=6.4Hz, 3H), 5.04(s, 2H), 5.17, 5.25(ABq, J=12.3Hz, 2H), 5.19(s, 2H).
IR ~ (CHC13) cm 1 1700, 1770.

?,~7~30 ~.os ~tep 2. Depro~ection To a solution of (lR,5S,6S)--2-[(3S,5S)-5-(1,1-dioxo-2-p-me-thoxybenzyloxycarbonyl 3,4,5,6-tetra-hydro-1,2,6-thiadiazin-6-yl)methyl-1-p-me-thoxy~enzyl-oxycarbonylp~rrolidin 3-yl]thio-6-[(lR)-1-hydroxy-ethyl]-1-mathyl-1-carba-2-penem-3-carboxylic acid p-methoxybenzylester (600 mg) in a mixture of dichloro-methane (9 ml) and nitromethane (3.5 ml) in a nitrogen atmosphere at -40~C, anisole (861 ~l) and a solution o~
l.OM aluminum chloride in nitromethane (5.94 ml) are added. The mix-ture is s-tirred at the same temperature for 1.5 hours. The reaction mixture is poured into a solution o~ sodium acetate (1.52 g) in water (50 ml), and washed with a mix-ture of ether and hexane. The aqueous layer is concentrated in vacuo to about 15 ml, and the mixture is puri~ied by styr~ne-divinylbenzene copolymer resin column chromatography to give (lR,5S,6S)-2-[(3S,5S)-5-(1,1-dioxo-3,4,5,6-tetrahydro-1,2,6-thiadiazin 2-yl)methylpyrrolidin-3-yl]-thio-6-[(lR)-1-hydroxy-ethyl]-1-methyl-1-carba-2-penem-3-carboxylic acid (190 mg'. Yield: 63%.
NMR ~ (D20) ppm: 1.20(d, J=7.2Hz, 3H), 1.27(d, J=6.4Hz, 3H), 1.65 to 1.80(m, 3H), 2.65 to 2.80(m, lH), 3.27 to 3.56(m, 9H), 3.64 to 3.74(m, lH), 3.91 to 4.10(m, 2H), 4.15 to ~.30(m, 2H).
IR ~ (KBr) cm~1: 3~00, 1750.
MIC ( ~ / ml): Staphylococcus aureus strain 3626:
25. Blood level: mice i.v., a~ter 15 min. ( ~ / ml):
28.4.

- ~.10 -Examples 10 to 12. Synthesis of (3R,5R), (3R,5S) and (3S,5R) pyrrolidylthiocarbapenem derivatives OH ~ OH
J~oP( OPh) J ~ S ~ SO2NH2 cooCHPh2 COOCHPh2 OH

2 ~N~ S
COOH

Step 1. Preparation of a protected pyrrolidylthiocar-bapenem derivatives To a solution of (lR,5S,6S)-2-diphenoxy~
phosphonyloxy-6-[(lR)-1-hydroxyethyl]-1-methyl-1-carba-2-penem-3-carboxylic acid diphenylmethyl ester (i.e., a substrate) and 1-t-butoxycarbonyl-2~(N-t-butoxyc~rbo-nyl-N-sulfamoylamino)methyl-4-mercaptopyrrolidine (Pyld) in acetonitrile (MeCN) under ice cooling, diiso-propylethylamine (HNPr-i) is added dropwise. The mixture is stirred to react under a condi-tion shown in Table 4. The reaction mixture is dilu-ted with ethyl acetate, and ice water is added thereto. The organic layer is taken, successively washed with water and saturated brine, dried over magnesium suifate, and concentrated in vacuo. The residue is purified by silica gel chromatography to give (lR,5S,6S)-2-[1-t-butoxycarbonyl-5-(N-t-butoxycarbonyl-N-sulfamoylamino) methylpyrrolidin-3-yl]-thio-6-[(lR)-l-hydroxyethyl]-1-methyl-1-carba-2-penem-3-carboxylic acid diphenylmethyl ester.

~ 0 ~ 3 Table 4 Configura-tion of Pyldi) HNPr-ij)MeCNk)Temp. Time Yield Example Pyld equiv. equiv. vol. min. %

Ex. 10 3R5R 1.17 1.30 7 ice 240 86 fold cooling Ex. 11 3R5S 1.20 1.30 7 ice 120 88 fold cooling Ex. 12 3S5R 1.14 1.27 7 ice 270 73 fold cooling i) Configuration of a pyrrolidine ring i) Molar ratio to the substrate k) The volume (ml) of the solvent to the weight (g) of the substrate .

.

Physieal propert:ies of the products (3R,5R) Isomer:
NMR ~ (CDC13) ppm: 1.26(d, J=7.2Hz, 3H), 1.39(d, 5J=6.2H~, 3H), 1.43 (s, 9H), 1.51(s, 9~l), 2.5~m, lH), 3.1 to 3.9(m, 6H), 4.0 to 4.7(m, ~H), 6.1(m,lH), 6.98(s, lH), 7.1 to 7.6(m, lOH).
IR ~ (KBr) cm 1 3400, 32~0, 1770, 1710, 1670.
(3S,5~) Isomer:
10NMR ~ (CDC13) ppm: 1;28(d, J=7.0~z, 3H), 1.36(s, 9~l), 1.40(d, J=6.2 H~, 3H), 1.52(s, 9H), 2.0(m, lH), 3.2 to 3.9(m, 7H), 4.2 to 4.4 (m, 2H), 4.4 to ~.6(m, lH), 6.01(s, 2H), 6.94(s, lH), 7.1 to 7.6(m, lOH).
IR ~ (KBr) em~l: 3400, 3240, 1772, 1708, 168Z.
(3R,5S) Isomer:
NMR ~ (CDCl3) ppm: 1.76(d, J=7.2Hz, 3H), 1.3 to 1.5(m, 12~i), 1.52(s, 9H), 1.9 to 2.1(m, lH), 3.2 to 3.9(m, 7H), 4.1 to 4.~(m, 2H), 4.4 -to 4.6(m, lH), 6.04~s, 2H), 6.94(s, lH), 7.1 to 7.6(m, lOH).
20IR ~ (~CBr) cm~l: 3~20, 1770, 1710.

Step 2. Deproteetion A solution of (lR,5S,6S)-2-[1-t-butoxy-carbonyl-5-(N-t-butoxyearbonyl-N-sulfamoylamino)methyl-pyrrolidin-3-yl]thio-6 [(lR)-l-hydroxyethyl]-l-methyl-l-earba-2-penem-3-earboxylie acid diphenylmethyl ester (i.e., a substrate) in diehloromethane (DClV5) is added dropwise in a nitrogen atmosphere into a solu-tion o~
aluminum chloride (AlCl3) in a mixture of dichlorometh-ane (DCM) and anisole (PhOMe). The mixture is stirredto reaet under the condition shown in Table 5. To the reaetion mixture, aqueous sodium acetate is addec. The aqueous layer is taken, washed with diehloromethane, ~07~3~J
- l:L3 -and purified by column chromatoyraphy over styrene-divinylbenzene copolymer resin to glve (lR,5S,6S)-6-[(lR)-1-hydroxyethyl]-2-[5-sulfamidomethylpyrrolidir-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid.

Table 5 Con-figura-tionl) AlC13m) DCMn) PhOMen)Temp. Tirne Yield Example of Pyld e~uiv. vol. vol. ~C min. %
(fold) (fold) Ex. lO 3R5R 8.0 16 10 -30 300 86 Ex. 11 3R5S 8.0 17 10 -30 150 88 Ex. 12 3S5R 8.0 17 10 -30 150 73 l) Configuration of a pyrrolidine ring m) Molar ratio to the substrate n) The volume (ml) of the solvent to the weight (g) of -the subs-trate 2~7~3~

Physical pr~perties of the produc-ts (3R,5R) Isomer:
NMR ~ (D20) ppm: 1.18(d, J=7.2Hz, 3H), 1.27(d, J=6.2Hz, 3H), l.9(m, lH), 2.7(m, lH), 3.2 to 3.6(m, 5H), 3.6 to 3.8(m, lH), 3.8 to ~nl(m, 2H), 4.2(m, 2H).
IR ~ (KBr) cm 1 3360, 1750.
(3S,5R) Isomer:
NMR ~ (CD3SOCD3) ppm: l.O9(d, J=7.0Hz, 3H), 1.14(d, J=6.2Hz, 3H), 1.7 to 2.0(m, lH), 1.9 to 2.2(m, lH), 2.9(m, lH), 3.0 to 3.3(m, 4H), 3.3 -to 3.6(m, lH), 3.6 to 3.8(m, 2H), 3~9(m, lH), 4.1(m, lH).
IR ~ (KBr) cm 1 3340, 1765, 1740, 1620, 1S75, 1548.
(3R,5S) Isomer:
NMR ~ (D20) ppm: 0.86(d, J=7.4Hz, 3H), 0.93(d, J=6 .4Hz, 3H), 2.43(d,J=6.4Hz, 3H), l.90(dd, J=9.OHz, J=4.4Hz, 2H), 2.9 to 3.3(m, 5H), 3.48(dd, J=13.2Hz, J=7.2Hz, lH), 3.7 to 3.8(m, 2H), 3.8 to 4.0 (m, 2H), 4.47 DHO.
I~ ~ (KBr) cm~l: 3400, 1750, 1585.

Example 13. Synthesis of a (3S,5S)-pyrrolidylthiocar-bapenem derivative using a monoallyloxycarbonyl in-t~rmediate HO Me Me3SiO Me Me3SiO Me ~~1 ~ 1 o~FN l\COSPh ~ , o~F~{P~(~Ph)2 COOCH2CH=CH2 CoocH2cH=cH2 COOCH2CH=CH2 OH ~ OH ~
3 os~ ~ ~ ,N~Alz 2 2 ~ ~ ~N ~ ~2~12 C~,H2CH=CH2 COOH

20~ X~

- 115 ~

Step 1 .Prepara-tion of a trimethylsilyl com~ound To a solu-tion of (3S,4S) 3-[(lR)-l-hydroxy-ethyl]-~-[(lR)-l-phenylthiocarbonylethyl]-l-allyloxy-carbonylmethyl-2-azetidinone (5.04 g: 13.35 mmole) in toluene (40 ml) under ice cooling, pyridine (1.51 ml-18.69 mmole) is added and trimethylchlorosilane (2.26 ml: 17.36 mmole) is added dropwise. The mixture is stirred at room temperature ~or 1.5 hours. Water (80 ml) is added to the reaction mixture, and aqueous layer is ex-tracted with toluene. The extract and the organic layer are combined, washed with water (2 times) and saturated brine, dried over magnesium sulfate, and concentrated in vacuo to give crude (3S,4S)-3-[(lR)-l-trimethylsi].yloxyethyl]-4-[(lR)-l-phenylthiocarbonyl~-ethyl]-1-allyloxycarbonylmethyl-2-azetidinone (5.614 g) as oily residue. Yield: 94%.

Step 2. Ring closure To a solution of the crude (3S,4S)-3-[(lR)-l-trimethylsilyloxyethyl]-~-[(lR)-l-phenylthiocarbonyl-ethyl]-l-allyloxycarbonylmethyl-2-azetidinone (5.60 S:
12.454 mmole) obtained in Step 1 in tetrahydrofuran (62 ml) at -60~C, a solution of lM-potassium t-butoxide (24.9 mmole) in tetrahydrofuran (24.9 ml ) iS added dropwise. The mixture is stirred for 10 minu-tes.
After adding iodome~hane (0.48 ml: 14.94 mmole) and stirring at the same temperature for 20 minutes, diphe-nylphosphoryl chloride (2.73 ml: 12.45 mmole) is added thereto. After allowing to warm to an ice water tem-perature over 1 hour, the reaction mixture is dilutedwith toluene (120 ml) and water (120 ml). The aqueous layer is extracted with toluene. The extract and the organic layer are combined, successively washed with ~7~3~

water (2 times), aqueous sodium hydrogen carbonate, and saturated brine, dried over magnesium sulfate, and concentrated in vacuo to give crude (lR,5S,6S)-2-diphe-noxyphospholyloxy-6-[(lR)-1-trimethylsilyloxyethy].]-1-methyl-1-carba-2-penem-3-carboxylic acid allyl ester (3.795 g) as oily residue. Yield: 104~.
IR ~ (Cl-ICl3) cm 1 3008, 1778, 1722, 1636, 1589, 1489.
NMR ~ (CDC13) ppm: 0.12(9H, s), 1.19(3H, d, J=7.2Hz), 1.25(3H, d, J=6.2Hz), 3.24(1H, dd, J=3.0Hz, J=6.8Hz), 3.3 to 3.6(1H, m), 4.11(1H, dd, J-3.0Hz, ~=10.2Hz), 4.1 to 4.3(1H, m), 4.6 to 4.7(2H, m), 5.1 to 5.5(2~1, m), 5.7 to 6.0(1H, m), 7.1 to 7.5(10H, m).

Step 3. Preparation of a protec-ted pyrrolidylthiocar-bapenem derivative To a solution of crude (lR,5S,6S)-2-diphenoxy-phospholyloxy-6-[(lR)-1-trimethylsilyloxy-ethyl]-1-methyl-1-carba-2-penem-3-carbo~ylic acid allyl ester (2.56 g: 4.2 mmole) obtained in Step 2 and (2S,4S)-1-allyloxycarbonyl-2~(N-sulfamoylamino)methyl-4-mercaptopyrrolidine (1.48 g: r .o mmole) in acetoni-trile (13 ml) under ice cooling, diisopropylethylamine (0.95 ml: 5.46 mmole) is added dropwise, and the mix-ture is stirred at the same temperature for 7.5 hours.
The reaction mixture is acidified with lN-hydrochloric acid (6.3 ml), stirred at the same temperature for 30 minutes, and ethyl acetate (80 ml) and ice water (80 ml) are added thereto. The organic layer is taken, successively washed with water and saturated brine, dried over magnesium sulfate and concentrated in vacuo.
The residue is purified by silica gel chromatography (toluene-ethyl acetate) to yive (lR,5S,6S)-2-[(3S,5S)-2~7~3~

I L~

l-allyloxycarbonyl-5-(N-sulfamoy].amino)methyl-pyrroli-din-3-yl~thio-6-[(lR)-l-hydroxye-t;hyl]-l~methyl-l~carba~
2-penem-3-carboxylic acid allyl ester (1.63 g).
Yield: 71~.
IR ~ (CHC13) cm 1 1772, 1691, 1410.
lHNMR ~ (CDC13) ppm: 1.26(3H, d, J=7.0Hz), 1.35(3H, d, J=6.0Hz), 1.7 to 2.7(3H, m), 3.1 to 3.5(5H, m), 3.5 to 3.8(1H, m), 3.9 to 4.4(4H, m), 4.5 to 4.9 (4H, m), 5.0 to 5.5(5H, m), 5.8 to 6.1(2H, m).
Step 4. Deprotection To a solution o:E (lR,5S,6S)~2-[(3S,5S)-l-allyloxycarbonyl-5-(N-sulfamoylamino) methylpyrrolidin-3~yl]thio-6-[(lR)-l-hydroxyethyl]-l-methyl-l-carba-2-penem-3-carboxylic acid allyl ester (379 mg:
0.695 mmole) in acetone (14 ml), triphenylphosphine (55 mg: 0.21 mmole) and tri-n-butyltin hydride (0.424 ml: 1.53 mmole) are added. Under ice cooling palladium tetrakis(triphenylphosphine) (81 mg:
0.07 mmole) is further added. After stirring at the same temperature for 45 minutes and at room temperature for 1 hour, water (35 ml) and methylene chloride (50 ml) are added to the reaction mixture. The aqueous layer is taken, washed with methylene chloride and lyophilized to give (lR,5S,6S)-6-[(lR)-l-hydroxyethyl]-2-[(3S,5S)-5-sulfamidomethylpyrrolidin-3-yl]~hio-1-methyl-l-carha-2-penem-3-carboxylic acid (238 mg).
Yield: 82%. (HPLC purity : 85%) NMR ~ (D20) ppm: 1.22(d, J~7.ZHz, 3H), 1.27(d, J=6.3Hz, 3H), 1.64 to 1.82(m, lH), 2.62 to 2.80(m, lH), 3.26 to 3.59(m, 5H), 3.63 to 3.76(m, lH), 3.g4 to 4.10(m, 2H), 4.16 to 4.29(m, 2H).
IR ~ (KBr) cm 1 340, 1750.

~P~6~3~

Example 14. Synthesis of a (3S,5S)-pyrrolidylthiocar bapenem derivative using a diallyloxycarbonyl interme-diate HJ ~ Me3Si~ Me Me3SiO
o~cN~ coSph ~ rN'~PO(OPh)2 COOC~I2CH=CH2 COO~2~=CH2 COOCH2~H=CH2 OH Me A1Z OH Me SO2~H2 J ~ ~NHSO2N~2 3 O~ N~ ~N~A1Z4 ~ o~C'N'~ ~H

Step 1. Preparation of a trimethylsilyl compound (3S,4S)-3-~(lR)-l-hydroxyethyl]-4-[(lR)-1-phenylthiocarbonylethyl]-1-allyloxycarbonylmethyl-2-azetidinone is trimethylsilyla-ted in the same manner as in Step 1 in Example 13 to give (3S,4S)-3-C(lR)-l-trimethylsilyloxyethyl~-4-[(lR)-1-phenylthiocarbonyl-eth~-l]-1-allyoxycarbonylmethyl-2-azetidinone.

Step 2. Ring closure The crude (3S,4S)-3-[(lR)-1-trimethylsilyl-oxyethyl]-4-[(lR)-l-phenylthiocarbonylethyl]-1-ally-loxy-carbonylmethyl-2-azetidinone obtained in Step 1 is allowed to react to close the ring in the similar manner as in Step 2 of Example 13 to give crude (lR,5S,6S)-2-diphenoxyphosphoryloxy-6-[(lR)-1-trime-th-ylsilyloxyethyl]-1-methyl-1-carba-2-penem-3-carboxylic acid allyl ester.

~7~43~

Step 3 Preparation of a protected pyrrolidylthio car-bapenem derivative Under similar reaction condition, -tne crude (lR,5S,6S)-2-diphenoxyphosphoryloxy-6-[(lR)-1-tri-methylsilyloxyethyl]-1-methyl-l-carba-2-penem-3-carbox-ylic acid allyl ester (5.05 g: 8.3 mmole) obtained in Step 2 and (2S,4S)-1-allyloxycarbonyl-2-(N-allyloxy-carbonyl-N-sulfamoylamino)methyl-g-mercaptopyrrolidine (3.77 g: 9.9~ mmole) are reacted to give (lR,5S,6S)-2-[(3S,5S)-1-allyloxycarbonyl-5-(N-allyloxycarbonyl-N~
sulfamoylamino)methylpyrrolidin-3-yl]thio-6-[(lR)-1-hydroxyethyl]-1-methyl-1-carba-2-penem-3-carboxylic acid allyl ester (3.65 g). Yield: 70%.
I~ ~ (CHC13) cm~1: 1777, 1718, 1686, 1395.
NMR ~ (CDC13) ppm: 1.27(3H, d, J=7.2Hz), 1.37(3H, d, J=6.2Hz), 2.5 to 2.7(1H, m), 3.1 to 3.3(3H, m), 3.6 to 3.8(2~, m), 4.0 to 4.3(4H, m), 4.4 to 4.9 (6H, m), 5.2 to 5.5(6H, m), 5.7 to 6.1(5H, m).

Step 4 Deprotection Under similar reaction condition to that in Step 4 in Example 13, (lR,5S,6S)-2-[(3S,5S)-1-allyloxy-carbonyl-~-(N-allyloxycarbonyl-N-sul~amoylamino)methyl-pyrrolidin-3-yl]thio-6-[(lR)-l-hydroxyethyl]-1-me~hyl-l~carba-2-penem-3-carboxylic acid allyl ester (369 mg:
0.586 mmole) is deprotected with triphenylphosphine (83 mg: 0.32 mmole), tri-n-butyltin hydride (0.64 ml:
2.3 mmole), and palladium te-trakis(triphenylphosphine) (122mg: 0.11 mmole) to give (lR, 5S,6S)-6-[('.R)-l-hydroxyethyl]-2-[(3S,5S)-5-sulfamidomethylpyrrolidin-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid (206 mg). Yield: 84~. (HPLC purity : 85%) 3 ~

~o NMR ~ (D20) ppm: 1.22(d, J=7.2Hz, 3H), 1.27(d, J=6.3Hz, 3El), 1.6~ to 1.82(m, lH), 2.62 to 2.80(m, lH), 3.26 to 3.59(m, 5H), 3.63 to 3.76(rn, lH), 3.8~ to 4.10(m, 2H), 4.16 to 4.29(m, 2H).
IR ~ (KBr) cm 1 3~00, 1750.

Example 15 A solution of (lR,5S,6S)-6-[(lR)-I-hydroxyethyl]-2-[(3S,5S)-5-sulfamidomethylphrrolidin-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid (0.5 g) in aqueous sodium hydrogen carbonate (5 ml) at pH 7.0 is filled in a vial (lO ml) and lyophilized.
The lyophilizate is dissolved in water for in~ectior (5 ml) be~ore use and in;ected thrice a day intrave~
nously to a patient suffering from urinary tract infec-tion caused by a sensitive strain of Staphylococcus aureus to cure the disease.

Example 16 A solution o~ (lR,5S,6S)-6-[(lR)-1-hydroxy-ethyl]-2-[(3S,5S)-5-(methylsulfamoyl)aminomethylpyrrol-idin-3-yl]thio-1-methyl-1-carba-2-penem-3-carboxylic acid (0.5 g) in aqueous sodium hydrogen carbonate (5 ml) at pH 7.0 is filled in a vial (10 ml) and lyop-hilized. The lyophilizate is dissolved in water for infection (5 ml) before use and injected thrice a day intravenously -to a patient sufferin~ from pneumonia caused by a sensitive strain of Klebsiella pneumoniae to treat the disease.
3n Example 17 A solution of (lR,5S,6S)-6-[(lR)-l-hydroxy-ethyl]-2-[(3S,5S)-5-(2-hydroxyethylsulfamoyl)amino-2076~3~

methylpyrrolidin~3-yl]th:io-1-methyl~1-carba-2-penem-3-carboxylic acid (2.0 g) in aqueous sodium hydrogen carbonate (10 ml) at pH 7.0 is filled in vial (100 ml) and lyophilized. The lyophilizate is dissolved in water for injection (50 ml) before use and administered by infusion four times a day intravenously to a patient severely suffering from the respiratory tract infection caused by a sensitive strain of Enterobacter cloacae to cure the disease.

Various o-ther modifications will be apparent to and can be readily made by those skilled in the art without departing from the scope and sp:Lrit of this invention. Accordingly, it is not intended that the scope of the claims appended hereto be limited to the description as set for-th herein, but rather that the claims be broadly construed.

Claims (22)

1. A pyrrolidylthiocarbapenem derivative represented by Formula I:

(I) wherein R1 is or lower alkyl; R2, R3 and R4 are hydrogen, lower alkyl which can be substituted, or an amino protecting group independently, or R2 and R3 together with a nitrogen atom to which R2 and R3 are bonded form a saturated or unsaturated cyclic group, or R2 and R4, or R3 and R4 together with two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclic group; each cyclic group can further include at least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be substituted; X1 is hydrogen or a hydroxy protecting group: X2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali metal or an alkaline-earth metal; and Y2 is hydrogen or an amino protecting group.

2. A pyrrolidylthiocarbapenem derivative according to claim 1, wherein R1 is methyl.

3. A pyrrolidylthiocarbapenem derivative according to claim 2, wherein R4 is hydrogen.

4. A pyrrolidylthiocarbapenem derivative according to claim 3, wherein X1 and Y2 are hydrogens and X2 is hydrogen or alkali metal.

5. A pyrrolidylthiocarbapenem derivative according to claim 4, wherein R2 and R3 are hydrogens; R2 is methyl and R3 are hydrogen; both R2 and R3 are methyl; or R2 is 2-hydroxyethyl, and R3 are hydrogen.

6. A pyrrolidylthiocarbapenem derivative according to claim 2, wherein R3 is hydrogen, and R2 and R4 are bonded to each other to form -CH2-CH2-.

7. A pyrrolidylthiocarbapenem derivative according to claim 2, wherein R3 is hydrogen, and R2 and R4 are bonded to each other to form -CH2-CH2-CH2-

8. A pyrrolidylthiocarbapenem derivative according to claim 1, wherein at least one group selected from the group consisting of R2, R3, R4 and Y2 is selected from the group consisting of t-butyloxy carbonyl, allyloxy-carbonyl, p-nitrobenzyloxycarbonyl, p-methoxybenzyl-oxycarbonyl and diazo.

9. A pyrrolidylthiocarbapenem derivative according to claim 1, wherein X1 is selected from the group consisting of hydrogen, trimethylsilyl, triethylsilyl and t-butoxydimethylsilyl.

10. A pyrrolidylthiocarbapenem derivative according to claim 1, wherein X2 is selected from the group consisting of hydrogen, sodium potassium, t-butyl, allyl, p-nitrobenzyl, p-methoxybenzyl and diphenylmethyl.

11. A pyrrolidylthiocarbapenem derivative according to claim 1, wherein the pyrrolidine ring in Formula I has a configulation of (3S,5S).

12. A pyrrolidine derivative represented by Formula II:

(II) wherein R2, R3 and R4 are hydrogen, lower alkyl which can be substituted, or an amino protecting group independently, or R2 and R3 together with a nitrogen atom to which R2 and R3 are bonded form a saturated or unsaturated cyclic group, or R2 and R4, or R3 and R4 together with two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclic group; each cyclic group can further include at least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be substituted; Y1 is hydrogen or a mercapto protecting group; and Y2 is hydrogen or an amino protecting group.

13. A pyrrolidine derivative according to claim 12, wherein R4 is hydrogen.

14. A method for producing a pyrrolidine derivative represented by Formula II:

(II) wherein R2, R3 and R4 are hydrogen, lower alkyl which can be substituted, or an amino protecting group independently, or R2 and R3 together with a nitrogen atom to which R2 and R3 are bonded form a saturated or unsaturated cyclic group, or R2 and R4, or R3 and R4 together with two nitrogen atoms and one sulfur atom in the sufamide group form a saturated or unsaturated cyclic group; each cyclic group can further include at least one atom selected from the group consisting of oxygen, sulfur and nitrogen, and each cyclic group can be substituted; Y1 is hydrogen or a mercapto protecting group; and Y2 is hydrogen or an amino protecting group;
the method comprising the steps of:
converting a hydroxy group at the 4-position of a 4-hydroxypyrrolidine-2-carboxylic acid derivative into a mercapto group;
converting a carboxy group at the 2-position into a hydroxymethyl group;
converting a hydroxy group in the hydroxy-methyl group into an amino group; and converting the amino group into a sulfamido group.

15. A method according to claim 14, wherein R4 is hydrogen.

16. A method for producing a pyrrolidylthiocarbapenem derivative comprising the step of:
allowing a carbapenem derivative to react with the pyrrolidine derivative of claim 12 to obtain the pyrrolidylthiocarbapenem derivative of claim 1;

the carbapenem derivative being represented by Formula III:

(III) wherein R1 is hydrogen or lower alkyl; X1 is hydrogen or a hydroxy protecting group; X2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali metal or an alkaline-earth metal; and X3 is a leaving group.

17. A method for producing a pyrrolidylthiocarbapenem derivative comprising the step of:
allowing a carbapenem derivative to react with the pyrrolidine derivative according to claim 13 to obtain the pyrrolidylthiocarbapenem derivative of claim 2;
the carbapenem derivative being represented by Formula III:

(III) wherein R1 is hydrogen or lower alkyl; X1 is hydrogen or a hydroxy protecting group; X2 is hydrogen, a carboxy protecting group, an ammonio group, an alkali metal or an alkaline-earth metal; and X3 is a leaving group.

18. An antibacterial agent comprising an effective amount of the pyrrolidylthiocarbapenem derivative of claim 1 as an active ingredient.

19. An antibacterial agent comprising an effective amount of the pyrrolidylthiocarbapenem derivative of claim 4 as an active ingredient.

20. An antibacterial agent comprising an effective amount of the pyrrolidylthiocarbapenem derivative of claim 5 as an active ingredient.

21. An antibacterial agent comprising an effective amount of the pyrrolidylthiocarbapenem derivative of claim 11 as an active ingredient.

22. A method for inhibiting growth of bacteria sensitive to the pyrrolidylthiocarbapenem derivative of claim 1 by allowing the sensitive bacterium to be in contact with an effective amount of the pyrrolidylthiocarbapenem derivative.