CA2021489A1 - Methods and compositions for chromosome-specific staining - Google Patents

Methods and compositions for chromosome-specific staining

Info

Publication number
CA2021489A1
CA2021489A1 CA 2021489 CA2021489A CA2021489A1 CA 2021489 A1 CA2021489 A1 CA 2021489A1 CA 2021489 CA2021489 CA 2021489 CA 2021489 A CA2021489 A CA 2021489A CA 2021489 A1 CA2021489 A1 CA 2021489A1
Authority
CA
Canada
Prior art keywords
methods
nucleic acid
probes
cytogenetic
acid probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2021489
Other languages
French (fr)
Other versions
CA2021489C (en
Inventor
Joe W. Gray
Daniel Pinkel
Douglas Tkachuk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
Joe W. Gray
Daniel Pinkel
Douglas Tkachuk
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joe W. Gray, Daniel Pinkel, Douglas Tkachuk, The Regents Of The University Of California filed Critical Joe W. Gray
Priority to CA002449414A priority Critical patent/CA2449414A1/en
Publication of CA2021489A1 publication Critical patent/CA2021489A1/en
Application granted granted Critical
Publication of CA2021489C publication Critical patent/CA2021489C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Abstract

Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses.
Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry.
Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications. Further provided is a method of preparing and applying chromosome-specific single-stranded nucleic acid probes to double stranded target DNA.
CA 2021489 1989-07-19 1990-07-18 Methods and compositions for chromosome-specific staining Expired - Lifetime CA2021489C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002449414A CA2449414A1 (en) 1989-07-19 1990-07-18 Methods and compositions for chromosome specific staining

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US38209489A 1989-07-19 1989-07-19
US382,094 1989-07-19
US44466989A 1989-12-01 1989-12-01
US444,669 1989-12-01
US49709890A 1990-03-20 1990-03-20
US497,098 1990-03-20

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002449414A Division CA2449414A1 (en) 1989-07-19 1990-07-18 Methods and compositions for chromosome specific staining

Publications (2)

Publication Number Publication Date
CA2021489A1 true CA2021489A1 (en) 1991-01-20
CA2021489C CA2021489C (en) 2004-01-27

Family

ID=27409560

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2021489 Expired - Lifetime CA2021489C (en) 1989-07-19 1990-07-18 Methods and compositions for chromosome-specific staining

Country Status (3)

Country Link
CA (1) CA2021489C (en)
IL (1) IL94906A (en)
PT (1) PT94751A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0500290A2 (en) * 1991-02-22 1992-08-26 Regents Of The University Of California Chromosome-specific staining to detect genetic rearrangements
US5512433A (en) * 1990-11-08 1996-04-30 Vysis, Inc. Methods and compounds for labeling DNA with xanthine and lower alkyl substituted xanthine derivatives and reagents for the in situ detection of chromosomes
US5698686A (en) * 1994-10-20 1997-12-16 Arch Development Corporation Yeast telomerase compositions
US6025126A (en) * 1991-10-28 2000-02-15 Arch Development Corporation Methods and compositions for the detection of chromosomal aberrations
US8021837B2 (en) 1992-03-04 2011-09-20 The Regents Of The University Of California Detection of chromosomal abnormalities associated with breast cancer
CN112680442A (en) * 2021-02-03 2021-04-20 河南省农业科学院 Genome specific probe for peanut wild species and use method

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512433A (en) * 1990-11-08 1996-04-30 Vysis, Inc. Methods and compounds for labeling DNA with xanthine and lower alkyl substituted xanthine derivatives and reagents for the in situ detection of chromosomes
EP0500290A2 (en) * 1991-02-22 1992-08-26 Regents Of The University Of California Chromosome-specific staining to detect genetic rearrangements
EP0500290A3 (en) * 1991-02-22 1993-03-03 The Regents Of The University Of California Chromosome-specific staining to detect genetic rearrangements
US6025126A (en) * 1991-10-28 2000-02-15 Arch Development Corporation Methods and compositions for the detection of chromosomal aberrations
US6576421B1 (en) 1991-10-28 2003-06-10 Arch Development Corporation Methods and compositions for the detection of chromosomal aberrations
US8021837B2 (en) 1992-03-04 2011-09-20 The Regents Of The University Of California Detection of chromosomal abnormalities associated with breast cancer
US5698686A (en) * 1994-10-20 1997-12-16 Arch Development Corporation Yeast telomerase compositions
US5916752A (en) * 1994-10-20 1999-06-29 Arch Development Corporation Telomerase screening methods
US6387619B1 (en) 1994-10-20 2002-05-14 Arch Development Telomerase compositions and methods
CN112680442A (en) * 2021-02-03 2021-04-20 河南省农业科学院 Genome specific probe for peanut wild species and use method
CN112680442B (en) * 2021-02-03 2024-03-08 河南省农业科学院 Peanut wild species genome specific probe and use method

Also Published As

Publication number Publication date
PT94751A (en) 1991-03-20
CA2021489C (en) 2004-01-27
IL94906A (en) 1995-10-31

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