CA2021489A1 - Methods and compositions for chromosome-specific staining - Google Patents
Methods and compositions for chromosome-specific stainingInfo
- Publication number
- CA2021489A1 CA2021489A1 CA 2021489 CA2021489A CA2021489A1 CA 2021489 A1 CA2021489 A1 CA 2021489A1 CA 2021489 CA2021489 CA 2021489 CA 2021489 A CA2021489 A CA 2021489A CA 2021489 A1 CA2021489 A1 CA 2021489A1
- Authority
- CA
- Canada
- Prior art keywords
- methods
- nucleic acid
- probes
- cytogenetic
- acid probes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses.
Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry.
Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications. Further provided is a method of preparing and applying chromosome-specific single-stranded nucleic acid probes to double stranded target DNA.
Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry.
Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications. Further provided is a method of preparing and applying chromosome-specific single-stranded nucleic acid probes to double stranded target DNA.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002449414A CA2449414A1 (en) | 1989-07-19 | 1990-07-18 | Methods and compositions for chromosome specific staining |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38209489A | 1989-07-19 | 1989-07-19 | |
US382,094 | 1989-07-19 | ||
US44466989A | 1989-12-01 | 1989-12-01 | |
US444,669 | 1989-12-01 | ||
US49709890A | 1990-03-20 | 1990-03-20 | |
US497,098 | 1990-03-20 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002449414A Division CA2449414A1 (en) | 1989-07-19 | 1990-07-18 | Methods and compositions for chromosome specific staining |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2021489A1 true CA2021489A1 (en) | 1991-01-20 |
CA2021489C CA2021489C (en) | 2004-01-27 |
Family
ID=27409560
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2021489 Expired - Lifetime CA2021489C (en) | 1989-07-19 | 1990-07-18 | Methods and compositions for chromosome-specific staining |
Country Status (3)
Country | Link |
---|---|
CA (1) | CA2021489C (en) |
IL (1) | IL94906A (en) |
PT (1) | PT94751A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0500290A2 (en) * | 1991-02-22 | 1992-08-26 | Regents Of The University Of California | Chromosome-specific staining to detect genetic rearrangements |
US5512433A (en) * | 1990-11-08 | 1996-04-30 | Vysis, Inc. | Methods and compounds for labeling DNA with xanthine and lower alkyl substituted xanthine derivatives and reagents for the in situ detection of chromosomes |
US5698686A (en) * | 1994-10-20 | 1997-12-16 | Arch Development Corporation | Yeast telomerase compositions |
US6025126A (en) * | 1991-10-28 | 2000-02-15 | Arch Development Corporation | Methods and compositions for the detection of chromosomal aberrations |
US8021837B2 (en) | 1992-03-04 | 2011-09-20 | The Regents Of The University Of California | Detection of chromosomal abnormalities associated with breast cancer |
CN112680442A (en) * | 2021-02-03 | 2021-04-20 | 河南省农业科学院 | Genome specific probe for peanut wild species and use method |
-
1990
- 1990-06-28 IL IL9490690A patent/IL94906A/en active IP Right Grant
- 1990-07-18 PT PT9475190A patent/PT94751A/en not_active Application Discontinuation
- 1990-07-18 CA CA 2021489 patent/CA2021489C/en not_active Expired - Lifetime
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5512433A (en) * | 1990-11-08 | 1996-04-30 | Vysis, Inc. | Methods and compounds for labeling DNA with xanthine and lower alkyl substituted xanthine derivatives and reagents for the in situ detection of chromosomes |
EP0500290A2 (en) * | 1991-02-22 | 1992-08-26 | Regents Of The University Of California | Chromosome-specific staining to detect genetic rearrangements |
EP0500290A3 (en) * | 1991-02-22 | 1993-03-03 | The Regents Of The University Of California | Chromosome-specific staining to detect genetic rearrangements |
US6025126A (en) * | 1991-10-28 | 2000-02-15 | Arch Development Corporation | Methods and compositions for the detection of chromosomal aberrations |
US6576421B1 (en) | 1991-10-28 | 2003-06-10 | Arch Development Corporation | Methods and compositions for the detection of chromosomal aberrations |
US8021837B2 (en) | 1992-03-04 | 2011-09-20 | The Regents Of The University Of California | Detection of chromosomal abnormalities associated with breast cancer |
US5698686A (en) * | 1994-10-20 | 1997-12-16 | Arch Development Corporation | Yeast telomerase compositions |
US5916752A (en) * | 1994-10-20 | 1999-06-29 | Arch Development Corporation | Telomerase screening methods |
US6387619B1 (en) | 1994-10-20 | 2002-05-14 | Arch Development | Telomerase compositions and methods |
CN112680442A (en) * | 2021-02-03 | 2021-04-20 | 河南省农业科学院 | Genome specific probe for peanut wild species and use method |
CN112680442B (en) * | 2021-02-03 | 2024-03-08 | 河南省农业科学院 | Peanut wild species genome specific probe and use method |
Also Published As
Publication number | Publication date |
---|---|
PT94751A (en) | 1991-03-20 |
CA2021489C (en) | 2004-01-27 |
IL94906A (en) | 1995-10-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |