CA2004068A1 - Hair analysis method - Google Patents
Hair analysis methodInfo
- Publication number
- CA2004068A1 CA2004068A1 CA 2004068 CA2004068A CA2004068A1 CA 2004068 A1 CA2004068 A1 CA 2004068A1 CA 2004068 CA2004068 CA 2004068 CA 2004068 A CA2004068 A CA 2004068A CA 2004068 A1 CA2004068 A1 CA 2004068A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- keratin
- enzyme
- mixture
- proteinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004458 analytical method Methods 0.000 title abstract 3
- 231100000640 hair analysis Toxicity 0.000 title 1
- 102000011782 Keratins Human genes 0.000 abstract 5
- 108010076876 Keratins Proteins 0.000 abstract 5
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 229940088598 enzyme Drugs 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 108091005804 Peptidases Proteins 0.000 abstract 2
- 102000035195 Peptidases Human genes 0.000 abstract 2
- 239000012491 analyte Substances 0.000 abstract 2
- 230000015556 catabolic process Effects 0.000 abstract 2
- 238000006731 degradation reaction Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 235000019833 protease Nutrition 0.000 abstract 2
- 108090001069 Chymopapain Proteins 0.000 abstract 1
- 108010067770 Endopeptidase K Proteins 0.000 abstract 1
- 108010059378 Endopeptidases Proteins 0.000 abstract 1
- 102000005593 Endopeptidases Human genes 0.000 abstract 1
- 108090000526 Papain Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- ISWQCIVKKSOKNN-UHFFFAOYSA-L Tiron Chemical compound [Na+].[Na+].OC1=CC(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C1O ISWQCIVKKSOKNN-UHFFFAOYSA-L 0.000 abstract 1
- 229960002976 chymopapain Drugs 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 abstract 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 abstract 1
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 abstract 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 abstract 1
- 210000004905 finger nail Anatomy 0.000 abstract 1
- 210000004209 hair Anatomy 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- PTLRDCMBXHILCL-UHFFFAOYSA-M sodium arsenite Chemical compound [Na+].[O-][As]=O PTLRDCMBXHILCL-UHFFFAOYSA-M 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 210000004906 toe nail Anatomy 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A method for the direct analysis of analyte in keratinized structures, e.g., hair, fingernails and toenails, which comprises preparing a mixture containing a low redox potential compound such as dithiothreitol or dithioerythritol, an enzyme suitable FOR
the degradation of the keratin structure and a sample of the keratin structure; permitting the enzyme to at least substantially degrade the sample of keratin structure, and subjecting the mixture to analysis to determine the identity and amount of analyte in the keratin substance sample. To accelerate the method, cupric sulfate or sodium arsenite may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention.
the degradation of the keratin structure and a sample of the keratin structure; permitting the enzyme to at least substantially degrade the sample of keratin structure, and subjecting the mixture to analysis to determine the identity and amount of analyte in the keratin substance sample. To accelerate the method, cupric sulfate or sodium arsenite may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/285,123 US5324642A (en) | 1987-12-28 | 1988-12-16 | Ligand assays of enzymatic hair digests |
| US285,123 | 1988-12-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2004068A1 true CA2004068A1 (en) | 1990-06-16 |
| CA2004068C CA2004068C (en) | 2001-06-19 |
Family
ID=23092819
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2004068 Expired - Lifetime CA2004068C (en) | 1988-12-16 | 1989-11-28 | Hair analysis method |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2004068C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0458594A2 (en) * | 1990-05-22 | 1991-11-27 | Psychemedics Corporation | Analysis of hair |
| EP0555440A1 (en) * | 1991-07-30 | 1993-08-18 | Psychemedics Corp | Hair analysis method. |
| WO1994018561A1 (en) * | 1993-02-03 | 1994-08-18 | Psychemedics Corporation | Hair analysis method |
| US5981204A (en) * | 1997-05-06 | 1999-11-09 | Johnson; Ted Donald | Method for forensically screening hair samples for the presence of cannabinoids |
-
1989
- 1989-11-28 CA CA 2004068 patent/CA2004068C/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5466579A (en) * | 1987-12-28 | 1995-11-14 | Psychemedics Corporation | Hair analysis method |
| US6949344B1 (en) * | 1987-12-28 | 2005-09-27 | Psychemical Corporation | Hair analysis method |
| EP0458594A2 (en) * | 1990-05-22 | 1991-11-27 | Psychemedics Corporation | Analysis of hair |
| EP0458594A3 (en) * | 1990-05-22 | 1992-10-07 | Psychemedics Corporation | Analysis of hair |
| EP0555440A1 (en) * | 1991-07-30 | 1993-08-18 | Psychemedics Corp | Hair analysis method. |
| EP0555440A4 (en) * | 1991-07-30 | 1994-03-23 | Psychemedics Corporation | |
| WO1994018561A1 (en) * | 1993-02-03 | 1994-08-18 | Psychemedics Corporation | Hair analysis method |
| US5981204A (en) * | 1997-05-06 | 1999-11-09 | Johnson; Ted Donald | Method for forensically screening hair samples for the presence of cannabinoids |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2004068C (en) | 2001-06-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |