CA1341290C - Method of genetically modifying plants - Google Patents
Method of genetically modifying plants Download PDFInfo
- Publication number
- CA1341290C CA1341290C CA000508796A CA508796A CA1341290C CA 1341290 C CA1341290 C CA 1341290C CA 000508796 A CA000508796 A CA 000508796A CA 508796 A CA508796 A CA 508796A CA 1341290 C CA1341290 C CA 1341290C
- Authority
- CA
- Canada
- Prior art keywords
- dna
- viral dna
- viral
- plant material
- plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 56
- 108020005202 Viral DNA Proteins 0.000 claims abstract description 67
- 108020004414 DNA Proteins 0.000 claims abstract description 50
- 239000000463 material Substances 0.000 claims abstract description 28
- 238000012546 transfer Methods 0.000 claims abstract description 24
- 244000005700 microbiome Species 0.000 claims abstract description 20
- 241000196324 Embryophyta Species 0.000 claims description 95
- 239000013612 plasmid Substances 0.000 claims description 28
- 241000700605 Viruses Species 0.000 claims description 27
- 241000589155 Agrobacterium tumefaciens Species 0.000 claims description 19
- 241000894006 Bacteria Species 0.000 claims description 18
- 239000013598 vector Substances 0.000 claims description 17
- 241000701489 Cauliflower mosaic virus Species 0.000 claims description 16
- 241000589158 Agrobacterium Species 0.000 claims description 13
- 210000004027 cell Anatomy 0.000 claims description 13
- 208000015181 infectious disease Diseases 0.000 claims description 11
- 239000002299 complementary DNA Substances 0.000 claims description 9
- 230000003612 virological effect Effects 0.000 claims description 7
- 102000053602 DNA Human genes 0.000 claims description 6
- 241000450599 DNA viruses Species 0.000 claims description 6
- 238000004113 cell culture Methods 0.000 claims description 6
- 244000075850 Avena orientalis Species 0.000 claims description 5
- 241000219198 Brassica Species 0.000 claims description 5
- 241000209094 Oryza Species 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- 241000726445 Viroids Species 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 231100000518 lethal Toxicity 0.000 claims description 5
- 230000001665 lethal effect Effects 0.000 claims description 5
- 235000011331 Brassica Nutrition 0.000 claims description 4
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- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- 241000209504 Poaceae Species 0.000 claims description 4
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- 235000007319 Avena orientalis Nutrition 0.000 claims description 3
- 241000219146 Gossypium Species 0.000 claims description 3
- 240000005979 Hordeum vulgare Species 0.000 claims description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000009973 maize Nutrition 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 241000234282 Allium Species 0.000 claims description 2
- 241000219317 Amaranthaceae Species 0.000 claims description 2
- 244000296825 Amygdalus nana Species 0.000 claims description 2
- 235000003840 Amygdalus nana Nutrition 0.000 claims description 2
- 244000099147 Ananas comosus Species 0.000 claims description 2
- 235000003911 Arachis Nutrition 0.000 claims description 2
- 244000105624 Arachis hypogaea Species 0.000 claims description 2
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- 241000234670 Bromeliaceae Species 0.000 claims description 2
- 240000001548 Camellia japonica Species 0.000 claims description 2
- 241000207199 Citrus Species 0.000 claims description 2
- 241000723377 Coffea Species 0.000 claims description 2
- 241000219104 Cucurbitaceae Species 0.000 claims description 2
- 241001507921 Cydonia Species 0.000 claims description 2
- 235000009807 Cydonia Nutrition 0.000 claims description 2
- 241000220485 Fabaceae Species 0.000 claims description 2
- 241000220223 Fragaria Species 0.000 claims description 2
- 241000702463 Geminiviridae Species 0.000 claims description 2
- 239000004471 Glycine Substances 0.000 claims description 2
- 235000009438 Gossypium Nutrition 0.000 claims description 2
- 241000208818 Helianthus Species 0.000 claims description 2
- 241000209219 Hordeum Species 0.000 claims description 2
- 241000234280 Liliaceae Species 0.000 claims description 2
- 241000220225 Malus Species 0.000 claims description 2
- 241000219071 Malvaceae Species 0.000 claims description 2
- 241000234295 Musa Species 0.000 claims description 2
- 241000234615 Musaceae Species 0.000 claims description 2
- 241000208125 Nicotiana Species 0.000 claims description 2
- 241000209117 Panicum Species 0.000 claims description 2
- 235000006443 Panicum miliaceum subsp. miliaceum Nutrition 0.000 claims description 2
- 235000009037 Panicum miliaceum subsp. ruderale Nutrition 0.000 claims description 2
- 241000218196 Persea Species 0.000 claims description 2
- 241000219833 Phaseolus Species 0.000 claims description 2
- 241000219843 Pisum Species 0.000 claims description 2
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- 241000220324 Pyrus Species 0.000 claims description 2
- 241001107098 Rubiaceae Species 0.000 claims description 2
- 241001092459 Rubus Species 0.000 claims description 2
- 241001093501 Rutaceae Species 0.000 claims description 2
- 241000209051 Saccharum Species 0.000 claims description 2
- 235000007238 Secale cereale Nutrition 0.000 claims description 2
- 235000005775 Setaria Nutrition 0.000 claims description 2
- 241000232088 Setaria <nematode> Species 0.000 claims description 2
- 241000207763 Solanum Species 0.000 claims description 2
- 235000002634 Solanum Nutrition 0.000 claims description 2
- 241001122767 Theaceae Species 0.000 claims description 2
- 241000219094 Vitaceae Species 0.000 claims description 2
- 241000209149 Zea Species 0.000 claims description 2
- 235000020971 citrus fruits Nutrition 0.000 claims description 2
- 235000018597 common camellia Nutrition 0.000 claims description 2
- 230000000977 initiatory effect Effects 0.000 claims description 2
- 235000014774 prunus Nutrition 0.000 claims description 2
- 244000000000 soil microbiome Species 0.000 claims description 2
- 240000006394 Sorghum bicolor Species 0.000 claims 2
- 241000219095 Vitis Species 0.000 claims 1
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- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 8
- 239000012634 fragment Substances 0.000 description 6
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- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 4
- 229960000268 spectinomycin Drugs 0.000 description 4
- 229960005322 streptomycin Drugs 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- 244000062793 Sorghum vulgare Species 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 241000218922 Magnoliophyta Species 0.000 description 2
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- 230000000813 microbial effect Effects 0.000 description 2
- 235000019713 millet Nutrition 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 2
- 229960001225 rifampicin Drugs 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 241001505951 Coconut cadang-cadang viroid Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000702489 Maize streak virus Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000025272 Persea americana Species 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 206010042566 Superinfection Diseases 0.000 description 1
- 241000723873 Tobacco mosaic virus Species 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000014599 transmission of virus Effects 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH2026/85-2 | 1985-05-13 | ||
| CH202685 | 1985-05-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1341290C true CA1341290C (en) | 2001-09-11 |
Family
ID=4224185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000508796A Expired - Fee Related CA1341290C (en) | 1985-05-13 | 1986-05-09 | Method of genetically modifying plants |
Country Status (13)
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3686633T2 (de) * | 1985-10-25 | 1993-04-15 | David Matthew Bisaro | Pflanzenvektoren. |
| GB8601680D0 (en) * | 1986-01-23 | 1986-02-26 | Agricultural Genetics Co | Modification of plant viruses |
| EP0267159A3 (de) * | 1986-11-07 | 1990-05-02 | Ciba-Geigy Ag | Verfahren zur genetischen Modifikation monokotyler Pflanzen |
| CA2230216A1 (en) | 1996-06-21 | 1997-12-24 | Monsanto Company | Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom |
| DE69841438D1 (de) | 1997-11-18 | 2010-02-25 | Pioneer Hi Bred Int | Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme |
| NZ504300A (en) | 1997-11-18 | 2002-06-28 | Pioneer Hi Bred Int | A method for directional stable transformation of eukaryotic cells using non-identical recombination sites |
| US7102055B1 (en) | 1997-11-18 | 2006-09-05 | Pioneer Hi-Bred International, Inc. | Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant |
| DE102020113705B4 (de) * | 2020-05-20 | 2024-09-12 | Lisa Dräxlmaier GmbH | Verfahren zum Herstellen eines Bauteils |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE52800T1 (de) * | 1983-01-13 | 1990-06-15 | Max Planck Gesellschaft | Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren. |
| WO1984002920A1 (en) * | 1983-01-17 | 1984-08-02 | Monsanto Co | Genetically transformed plants |
| EP0126546B2 (en) * | 1983-04-15 | 1994-03-30 | Lubrizol Genetics Inc. | Plant structural gene expression |
| US4646527A (en) * | 1985-10-22 | 1987-03-03 | Taylor Shelton E | Refrigerant recovery and purification system |
| DE3686633T2 (de) * | 1985-10-25 | 1993-04-15 | David Matthew Bisaro | Pflanzenvektoren. |
-
1986
- 1986-05-09 DD DD86290094A patent/DD279503A5/de not_active IP Right Cessation
- 1986-05-09 GB GB8611356A patent/GB2174995B/en not_active Expired
- 1986-05-09 CA CA000508796A patent/CA1341290C/en not_active Expired - Fee Related
- 1986-05-09 AR AR86303901A patent/AR243235A1/es active
- 1986-05-12 EP EP86106440A patent/EP0201904B1/de not_active Expired - Lifetime
- 1986-05-12 HU HU861955A patent/HU205388B/hu unknown
- 1986-05-12 ZA ZA863485A patent/ZA863485B/xx unknown
- 1986-05-12 AT AT86106440T patent/ATE88504T1/de not_active IP Right Cessation
- 1986-05-12 DE DE86106440T patent/DE3688301D1/de not_active Expired - Lifetime
- 1986-05-12 IL IL78761A patent/IL78761A/xx not_active IP Right Cessation
- 1986-05-12 BR BR8602126A patent/BR8602126A/pt not_active Application Discontinuation
- 1986-05-12 AU AU57356/86A patent/AU595527B2/en not_active Expired
- 1986-05-13 JP JP61109363A patent/JP3057204B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| GB8611356D0 (en) | 1986-06-18 |
| IL78761A0 (en) | 1986-08-31 |
| JP3057204B2 (ja) | 2000-06-26 |
| AU595527B2 (en) | 1990-04-05 |
| EP0201904B1 (de) | 1993-04-21 |
| GB2174995A (en) | 1986-11-19 |
| BR8602126A (pt) | 1987-01-13 |
| AU5735686A (en) | 1986-11-20 |
| ZA863485B (en) | 1987-01-28 |
| EP0201904A1 (de) | 1986-11-20 |
| IL78761A (en) | 1991-07-18 |
| ATE88504T1 (de) | 1993-05-15 |
| HU205388B (en) | 1992-04-28 |
| DD279503A5 (de) | 1990-06-06 |
| JPS61260886A (ja) | 1986-11-19 |
| HUT41939A (en) | 1987-06-29 |
| AR243235A1 (es) | 1993-07-30 |
| GB2174995B (en) | 1989-07-05 |
| DE3688301D1 (enrdf_load_stackoverflow) | 1993-05-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |