CA1297406C - Methode pour la detection et la quantification des ligands dans des liquides - Google Patents

Methode pour la detection et la quantification des ligands dans des liquides

Info

Publication number
CA1297406C
CA1297406C CA000556618A CA556618A CA1297406C CA 1297406 C CA1297406 C CA 1297406C CA 000556618 A CA000556618 A CA 000556618A CA 556618 A CA556618 A CA 556618A CA 1297406 C CA1297406 C CA 1297406C
Authority
CA
Canada
Prior art keywords
biotin
ligand
interest
avidin
labeled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA000556618A
Other languages
English (en)
Inventor
Richard C. Hevey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/013,581 external-priority patent/US4863876A/en
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of CA1297406C publication Critical patent/CA1297406C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA000556618A 1987-01-15 1988-01-15 Methode pour la detection et la quantification des ligands dans des liquides Expired - Lifetime CA1297406C (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US356687A 1987-01-15 1987-01-15
US360487A 1987-01-15 1987-01-15
US003,604 1987-01-15
US003,566 1987-01-15
US013,581 1987-02-11
US07/013,581 US4863876A (en) 1987-01-15 1987-02-11 Method of detecting and quantifying ligands in liquids via biotin-avidin-medicated fluorescence polarization

Publications (1)

Publication Number Publication Date
CA1297406C true CA1297406C (fr) 1992-03-17

Family

ID=27357435

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000556618A Expired - Lifetime CA1297406C (fr) 1987-01-15 1988-01-15 Methode pour la detection et la quantification des ligands dans des liquides

Country Status (4)

Country Link
JP (1) JPH02502122A (fr)
AU (1) AU1342488A (fr)
CA (1) CA1297406C (fr)
WO (1) WO1988005538A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE64286B1 (en) * 1988-05-25 1995-07-26 Boehringer Mannheim Gmbh Process for the determination of an immunologically detectable substance and a suitable vessel therefor
DE3834766A1 (de) * 1988-10-12 1990-04-26 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
US6087188A (en) * 1992-11-13 2000-07-11 Alk A/S Two-site immunoassay for an antibody with chemiluminescent label and biotin bound ligand
US6503709B1 (en) 1997-07-03 2003-01-07 Id Biomedical Corporation Methods for rapidly detecting methicillin resistant staphylococci
JP4193055B2 (ja) 2001-09-19 2008-12-10 積水メディカル株式会社 発光性高分子化合物、及びそのバイオアッセイにおける利用
WO2016183092A1 (fr) * 2015-05-11 2016-11-17 Access Medical Systems, Ltd. Procédé de réutilisation d'une sonde d'essai et de réactifs dans un immunodosage

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0201211A1 (fr) * 1985-04-10 1986-11-12 Whittaker Corporation Méthode et compositions pour immuno-essais visuels à phase solide basés sur des particules microsphériques et luminescente

Also Published As

Publication number Publication date
AU1342488A (en) 1988-08-10
WO1988005538A1 (fr) 1988-07-28
JPH02502122A (ja) 1990-07-12

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