CA1294871C - Immunoassay reagents, kits and methods - Google Patents
Immunoassay reagents, kits and methodsInfo
- Publication number
- CA1294871C CA1294871C CA000547275A CA547275A CA1294871C CA 1294871 C CA1294871 C CA 1294871C CA 000547275 A CA000547275 A CA 000547275A CA 547275 A CA547275 A CA 547275A CA 1294871 C CA1294871 C CA 1294871C
- Authority
- CA
- Canada
- Prior art keywords
- cells
- enzyme
- antigen
- viable
- hapten
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 37
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 26
- 238000003018 immunoassay Methods 0.000 title claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 57
- 108090000790 Enzymes Proteins 0.000 claims abstract description 57
- 239000003550 marker Substances 0.000 claims abstract description 26
- 238000009739 binding Methods 0.000 claims abstract description 20
- 238000012360 testing method Methods 0.000 claims abstract description 20
- 230000001580 bacterial effect Effects 0.000 claims abstract description 19
- 230000027455 binding Effects 0.000 claims abstract description 19
- 108060003951 Immunoglobulin Proteins 0.000 claims abstract description 18
- 102000018358 immunoglobulin Human genes 0.000 claims abstract description 18
- 239000007900 aqueous suspension Substances 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 7
- 238000011065 in-situ storage Methods 0.000 claims abstract description 5
- 239000000427 antigen Substances 0.000 claims description 36
- 102000036639 antigens Human genes 0.000 claims description 35
- 108091007433 antigens Proteins 0.000 claims description 35
- 108090000623 proteins and genes Proteins 0.000 claims description 27
- 239000007787 solid Substances 0.000 claims description 21
- 239000000725 suspension Substances 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 14
- 108020003175 receptors Proteins 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 241000191967 Staphylococcus aureus Species 0.000 claims description 8
- 102000016938 Catalase Human genes 0.000 claims description 7
- 108010053835 Catalase Proteins 0.000 claims description 7
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 7
- 230000000717 retained effect Effects 0.000 claims description 6
- 230000006641 stabilisation Effects 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000000159 protein binding assay Methods 0.000 claims description 4
- 239000000969 carrier Substances 0.000 claims description 3
- 230000002255 enzymatic effect Effects 0.000 claims description 3
- 230000009870 specific binding Effects 0.000 claims description 3
- 108010006464 Hemolysin Proteins Proteins 0.000 claims description 2
- 239000002250 absorbent Substances 0.000 claims description 2
- 230000002745 absorbent Effects 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000003228 hemolysin Substances 0.000 claims description 2
- 230000009083 hemolysis by symbiont of host erythrocytes Effects 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 7
- 210000004027 cell Anatomy 0.000 description 51
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 17
- 239000002953 phosphate buffered saline Substances 0.000 description 17
- 102000004169 proteins and genes Human genes 0.000 description 16
- 102000005962 receptors Human genes 0.000 description 9
- 238000003556 assay Methods 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
- 210000002421 cell wall Anatomy 0.000 description 6
- 101710120037 Toxin CcdB Proteins 0.000 description 5
- 229940072221 immunoglobulins Drugs 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 3
- 102000009109 Fc receptors Human genes 0.000 description 3
- 108010087819 Fc receptors Proteins 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 241000191940 Staphylococcus Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 239000001974 tryptic soy broth Substances 0.000 description 3
- OPEGYZAATHKDEM-HCWXCVPCSA-N (2r,4s)-2-[(r)-carboxy(formamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid Chemical compound CC1(C)S[C@H]([C@H](NC=O)C(O)=O)N[C@H]1C(O)=O OPEGYZAATHKDEM-HCWXCVPCSA-N 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 108091008324 binding proteins Proteins 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 239000000700 radioactive tracer Substances 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 238000009007 Diagnostic Kit Methods 0.000 description 1
- YCAGGFXSFQFVQL-UHFFFAOYSA-N Endothion Chemical compound COC1=COC(CSP(=O)(OC)OC)=CC1=O YCAGGFXSFQFVQL-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 108010087702 Penicillinase Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000295644 Staphylococcaceae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 108700011201 Streptococcus IgG Fc-binding Proteins 0.000 description 1
- 101150030413 Timp gene Proteins 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 239000008098 formaldehyde solution Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 231100000206 health hazard Toxicity 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229940027941 immunoglobulin g Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- XMBWDFGMSWQBCA-RNFDNDRNSA-M iodine-131(1-) Chemical compound [131I-] XMBWDFGMSWQBCA-RNFDNDRNSA-M 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229950009506 penicillinase Drugs 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000011272 standard treatment Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- -1 test tubes Substances 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56938—Staphylococcus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL80083A IL80083A0 (en) | 1986-09-19 | 1986-09-19 | Immunoassay reagents,kits and methods |
| IL80083 | 1986-09-19 | ||
| IL80313A IL80313A0 (en) | 1986-09-19 | 1986-10-15 | Immunoassay reagents,kits and methods |
| IL80313 | 1986-10-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1294871C true CA1294871C (en) | 1992-01-28 |
Family
ID=26321590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000547275A Expired - Lifetime CA1294871C (en) | 1986-09-19 | 1987-09-18 | Immunoassay reagents, kits and methods |
Country Status (9)
| Country | Link |
|---|---|
| AU (1) | AU594503B2 (enrdf_load_stackoverflow) |
| CA (1) | CA1294871C (enrdf_load_stackoverflow) |
| CH (1) | CH675309A5 (enrdf_load_stackoverflow) |
| DE (1) | DE3731227A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2604259B1 (enrdf_load_stackoverflow) |
| GB (1) | GB2197468B (enrdf_load_stackoverflow) |
| IL (1) | IL80313A0 (enrdf_load_stackoverflow) |
| IT (1) | IT1223303B (enrdf_load_stackoverflow) |
| NL (1) | NL8702235A (enrdf_load_stackoverflow) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989010974A1 (en) * | 1988-05-11 | 1989-11-16 | Trustees Of The Sisters Of Charity Of Australia | Enzyme immunoassay system |
| US5759774A (en) * | 1988-05-18 | 1998-06-02 | Cobe Laboratories, Inc. | Method of detecting circulating antibody types using dried or lyophilized cells |
| DE3936256A1 (de) * | 1989-10-31 | 1991-05-02 | Max Planck Gesellschaft | Test zur differentiellen quantifizierung des freien bzw. des peptidasekomplexierten proteinaseinhibitors (alpha)(pfeil abwaerts)2(pfeil abwaerts)-makroglobulin |
| US20100062418A1 (en) * | 2006-11-22 | 2010-03-11 | Mach Patrick A | Inactivated and dried biological preparations |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3990947A (en) * | 1975-03-07 | 1976-11-09 | Warner-Lambert Company | Composition for detecting fibrinogen, fibrinogen split products and fibrin split products |
| FR2405301A1 (fr) * | 1977-10-04 | 1979-05-04 | Api Labor | Substrats et procede permettant l'identification rapide de bacteries du genre streptococcus |
| SE7712244L (sv) * | 1977-10-31 | 1979-05-01 | Jonsson U R S | Forfarande for framstellning av preparationer av avdodade bakterier |
| US4471058A (en) * | 1982-07-26 | 1984-09-11 | Board Of Trustees Operating Michigan State University | Method for the detection and/or determination of a polyvalent antigen using at least two different monoclonal antibodies |
-
1986
- 1986-10-15 IL IL80313A patent/IL80313A0/xx unknown
-
1987
- 1987-09-10 GB GB8721276A patent/GB2197468B/en not_active Expired - Lifetime
- 1987-09-16 CH CH3575/87A patent/CH675309A5/fr not_active IP Right Cessation
- 1987-09-17 IT IT21950/87A patent/IT1223303B/it active
- 1987-09-17 DE DE19873731227 patent/DE3731227A1/de not_active Withdrawn
- 1987-09-17 FR FR878712904A patent/FR2604259B1/fr not_active Expired - Lifetime
- 1987-09-17 AU AU78623/87A patent/AU594503B2/en not_active Ceased
- 1987-09-18 CA CA000547275A patent/CA1294871C/en not_active Expired - Lifetime
- 1987-09-18 NL NL8702235A patent/NL8702235A/nl not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| IL80313A0 (en) | 1987-01-30 |
| GB8721276D0 (en) | 1987-10-14 |
| AU594503B2 (en) | 1990-03-08 |
| CH675309A5 (enrdf_load_stackoverflow) | 1990-09-14 |
| IT8721950A0 (it) | 1987-09-17 |
| AU7862387A (en) | 1988-03-24 |
| IT1223303B (it) | 1990-09-19 |
| GB2197468B (en) | 1991-03-13 |
| FR2604259A1 (fr) | 1988-03-25 |
| DE3731227A1 (de) | 1988-06-30 |
| GB2197468A (en) | 1988-05-18 |
| FR2604259B1 (fr) | 1991-05-10 |
| NL8702235A (nl) | 1988-04-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU611098B2 (en) | Connector and a disposable assembly utilizing said connector | |
| US4935339A (en) | Delayed solid phase immunologic assay | |
| US5126241A (en) | Process for the determination of a specifically bindable substance | |
| US6489129B1 (en) | Antigen-specific IgM detection | |
| EP0124352A2 (en) | Protected binding assay | |
| US4686181A (en) | Specific binding assay employing anti-G6PDH as label | |
| EP0315364A2 (en) | Simultaneous immunoassay for the determination of antigens and antibodies | |
| JPH01227061A (ja) | イオン捕捉イムノアッセイ法および装置 | |
| IE50457B1 (en) | Method for detecting and determining proteinaceous specific binding agents and materials bindable thereto,test composition and test kit therefor | |
| JP6947780B2 (ja) | 標的物質の検出用試薬、検出方法および標的物質を検出するために用いられる担体ならびにその製造方法 | |
| EP0348211A2 (en) | Visual discrimination qualitative enzyme assay | |
| US4410634A (en) | Method of passively adsorbing immuno-reactive haptens to solid phases | |
| EP0028132A2 (en) | Method of passively adsorbing immuno-reactive haptens to solid phases | |
| Burgett et al. | A solid phase fluorescent immunoassay for the quantitation of the C4 component of human complement | |
| CA1294871C (en) | Immunoassay reagents, kits and methods | |
| CA2359234C (en) | A method for labeled conjugate production | |
| US6207398B1 (en) | Cyclosporine derivatives and uses thereof | |
| CA1335173C (en) | Solid-phase non-separation enzyme assay | |
| CA1146856A (en) | Method of detecting and quantitating haptens and antigens | |
| EP0362284A1 (en) | Multiple antigen immunoassay | |
| US4971916A (en) | Liposome based homogeneous immunoassay for diagnostic tests | |
| JPH03503566A (ja) | 天然結合タンパク質に対するモノクローナル抗体を用いたイムノアツセイ | |
| WO1989010974A1 (en) | Enzyme immunoassay system | |
| JP4068259B2 (ja) | 免疫測定方法及び乾式免疫測定試薬 | |
| EP0301333A2 (en) | Liposome based homogeneous immunoassay for diagnostic tests |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |