CA1229549A - Immunoassay for human chorionic gonadotropin - Google Patents
Immunoassay for human chorionic gonadotropinInfo
- Publication number
- CA1229549A CA1229549A CA000452265A CA452265A CA1229549A CA 1229549 A CA1229549 A CA 1229549A CA 000452265 A CA000452265 A CA 000452265A CA 452265 A CA452265 A CA 452265A CA 1229549 A CA1229549 A CA 1229549A
- Authority
- CA
- Canada
- Prior art keywords
- hcg
- beta
- immunoassay
- subunit
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000003018 immunoassay Methods 0.000 title claims abstract description 45
- 102000011022 Chorionic Gonadotropin Human genes 0.000 title abstract description 5
- 108010062540 Chorionic Gonadotropin Proteins 0.000 title abstract description 5
- 229940084986 human chorionic gonadotropin Drugs 0.000 title abstract description 5
- 210000002700 urine Anatomy 0.000 claims abstract description 25
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims abstract description 23
- 210000002966 serum Anatomy 0.000 claims abstract description 13
- 239000011159 matrix material Substances 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 10
- 206010028980 Neoplasm Diseases 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 229920002684 Sepharose Polymers 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 229920000936 Agarose Polymers 0.000 claims 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims 1
- 208000024313 Testicular Neoplasms Diseases 0.000 claims 1
- 206010057644 Testis cancer Diseases 0.000 claims 1
- 201000003120 testicular cancer Diseases 0.000 claims 1
- 102100026328 Ciliogenesis and planar polarity effector 1 Human genes 0.000 description 79
- 238000003556 assay Methods 0.000 description 26
- 238000002360 preparation method Methods 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 230000027455 binding Effects 0.000 description 5
- 238000009739 binding Methods 0.000 description 5
- 231100000673 dose–response relationship Toxicity 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 208000000995 spontaneous abortion Diseases 0.000 description 5
- 108010062580 Concanavalin A Proteins 0.000 description 4
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 230000009027 insemination Effects 0.000 description 4
- 206010000234 Abortion spontaneous Diseases 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 102000006395 Globulins Human genes 0.000 description 3
- 108010044091 Globulins Proteins 0.000 description 3
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 3
- 241000282320 Panthera leo Species 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 210000004408 hybridoma Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 208000037062 Polyps Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000009260 cross reactivity Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 201000003511 ectopic pregnancy Diseases 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 229960004198 guanidine Drugs 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 208000000509 infertility Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000009871 nonspecific binding Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 238000009877 rendering Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- FJQZXCPWAGYPSD-UHFFFAOYSA-N 1,3,4,6-tetrachloro-3a,6a-diphenylimidazo[4,5-d]imidazole-2,5-dione Chemical compound ClN1C(=O)N(Cl)C2(C=3C=CC=CC=3)N(Cl)C(=O)N(Cl)C12C1=CC=CC=C1 FJQZXCPWAGYPSD-UHFFFAOYSA-N 0.000 description 1
- 229910052695 Americium Inorganic materials 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 108010032597 Cohn fraction II Proteins 0.000 description 1
- 102000009151 Luteinizing Hormone Human genes 0.000 description 1
- 108010073521 Luteinizing Hormone Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 101100118976 Mus musculus Clint1 gene Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- XOJVVFBFDXDTEG-UHFFFAOYSA-N Norphytane Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)C XOJVVFBFDXDTEG-UHFFFAOYSA-N 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- JQYMGXZJTCOARG-UHFFFAOYSA-N Reactive blue 2 Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC(S(O)(=O)=O)=C1 JQYMGXZJTCOARG-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- LXQXZNRPTYVCNG-UHFFFAOYSA-N americium atom Chemical compound [Am] LXQXZNRPTYVCNG-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000026045 iodination Effects 0.000 description 1
- 238000006192 iodination reaction Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229940040129 luteinizing hormone Drugs 0.000 description 1
- HOVAGTYPODGVJG-VEIUFWFVSA-N methyl alpha-D-mannoside Chemical compound CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]1O HOVAGTYPODGVJG-VEIUFWFVSA-N 0.000 description 1
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000001817 pituitary effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 150000003440 styrenes Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57476—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving oncofetal proteins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Endocrinology (AREA)
- Oncology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Hospice & Palliative Care (AREA)
- Reproductive Health (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US49221083A | 1983-05-06 | 1983-05-06 | |
| US492,210 | 1983-05-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1229549A true CA1229549A (en) | 1987-11-24 |
Family
ID=23955381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000452265A Expired CA1229549A (en) | 1983-05-06 | 1984-04-18 | Immunoassay for human chorionic gonadotropin |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0146563A4 (ja) |
| JP (1) | JPS60501271A (ja) |
| CA (1) | CA1229549A (ja) |
| WO (1) | WO1984004598A1 (ja) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6238362A (ja) * | 1985-08-12 | 1987-02-19 | Chemo Sero Therapeut Res Inst | Ｔｓｈの測定方法 |
| US4933275A (en) * | 1985-10-24 | 1990-06-12 | The General Hospital Corporation | Method for the detection of a polypeptide subunit in the presence of a quaternary protein containing the subunit |
| JPS63127160A (ja) * | 1986-06-30 | 1988-05-31 | Sukeyasu Yoneda | 特異的蛋白質の検出方法 |
| JPS63305251A (ja) * | 1987-06-05 | 1988-12-13 | Dai Ichi Pure Chem Co Ltd | ラテツクス凝集反応を利用する免疫学的測定方法 |
| CA2040664A1 (en) * | 1990-05-11 | 1991-11-12 | Harold C. Warren, Iii | Immunoreagent composition, test kit and rapid assay for human chorionic gonadotropin |
| AU5136100A (en) * | 1999-05-17 | 2000-12-05 | Avi Biopharma, Inc. | Combined approach to treatment of cancer with hcg vaccines |
| GB201402668D0 (en) * | 2014-02-14 | 2014-04-02 | Concepta Diagnostics Ltd | Fertility and pregnancy monitoring device and method |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4048298A (en) * | 1975-02-25 | 1977-09-13 | Rohm And Haas Company | Solid phase double-antibody radioimmunoassay procedure |
| GB1572220A (en) * | 1976-10-07 | 1980-07-30 | Mochida Pharm Co Ltd | Immunochemical process of measuring physiologically active substances |
| US4098876A (en) * | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
| US4244940A (en) * | 1978-09-05 | 1981-01-13 | Bio-Rad Laboratories, Inc. | Single-incubation two-site immunoassay |
| CH642458A5 (en) * | 1980-04-25 | 1984-04-13 | Hoffmann La Roche | Immunological method |
| US4376110A (en) * | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
| ES511156A0 (es) * | 1981-04-13 | 1983-08-01 | Hoechst Co American | Un metodo de determinar la presencia de un antigeno en un medio liquido sospechoso de contenerlo. |
-
1984
- 1984-04-13 EP EP19840901866 patent/EP0146563A4/en not_active Ceased
- 1984-04-13 WO PCT/US1984/000572 patent/WO1984004598A1/en not_active Application Discontinuation
- 1984-04-13 JP JP50209384A patent/JPS60501271A/ja active Pending
- 1984-04-18 CA CA000452265A patent/CA1229549A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| WO1984004598A1 (en) | 1984-11-22 |
| JPS60501271A (ja) | 1985-08-08 |
| EP0146563A1 (en) | 1985-07-03 |
| EP0146563A4 (en) | 1988-11-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4851356A (en) | Immunoassay for human chorionic gonadotropin | |
| Armstrong et al. | Use of a highly sensitive and specific immunoradiometric assay for detection of human chorionic gonadotropin in urine of normal, nonpregnaiit, and pregnant individuals | |
| Klug et al. | Monoclonal antibody immunoradiometric assay for an antigenic determinant (CA 125) associated with human epithelial ovarian carcinomas | |
| Goldsmith | Radioimmunoassay: Review of basic principles | |
| O'Connor et al. | Development of highly sensitive immunoassays to measure human chorionic gonadotropin, its β-subunit, and β core fragment in the urine: application to malignancies | |
| US4514505A (en) | Monoclonal antibody mixtures and use thereof for enhanced sensitivity immunoassays | |
| US4232001A (en) | Methods and materials for detection of estrophilin | |
| JP2606722B2 (ja) | 超高感度抗原物質の測定法 | |
| Wilde et al. | A sensitive radioimmunoassay for human chorionic gonadotrophin and luteinizing hormone | |
| Norman et al. | Monoclonal antibodies to human chorionic gonadotropin: implications for antigenic mapping, immunoradiometric assays, and clinical applications | |
| JP2006523298A (ja) | 部分的ｐｒｏＡＮＰペプチドを同定するためのサンドイッチイムノアッセイ | |
| CA1229549A (en) | Immunoassay for human chorionic gonadotropin | |
| Schroeder et al. | Specificity of human beta-choriogonadotropin assays for the hormone and for an immunoreactive fragment present in urine during normal pregnancy. | |
| US4693969A (en) | Reagent for use in a sandwich solid-phase enzyme-immunoassay and process for employing same | |
| US5236846A (en) | Ectopic pregnancy test | |
| CA1073812A (en) | Thyroxine binding globulin assay using radiolabeled thyroxine and immobilized anti-tbg antibodies | |
| Kelsey et al. | An enzyme immunoassay for the detection of human tumor nucleolar antigens | |
| Joshi et al. | Serum and peritoneal fluid proteins in women with and without endometriosis | |
| Miller et al. | Chemiluminescence immunoassay for progesterone in plasma incorporating acridinium ester labelled antigen | |
| Van Orden et al. | Radioimmunoassay of PGE and an approach to the specific measurement of PGE1 | |
| OHTAKI et al. | Radioimmunoassay of saliva estrone sulfate in pregnant sows | |
| Schanbacher et al. | Radioimmunoassay of ovine and bovine serum progesterone without extraction and chromatography | |
| Dericks-Tan et al. | Pseudohypergonadotropinemia and pseudohyperprolactinemia induced by heterophilic antibodies? | |
| Lai et al. | Radioimmunassay of ovine alpha-fetoprotein | |
| HABER et al. | The application of antibody to the measurement of substances of physiological and pharmacological interest |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |