BR112022007622A2 - Sistemas, métodos e dispositivos para eletroporação de fluido contendo células - Google Patents

Sistemas, métodos e dispositivos para eletroporação de fluido contendo células

Info

Publication number
BR112022007622A2
BR112022007622A2 BR112022007622A BR112022007622A BR112022007622A2 BR 112022007622 A2 BR112022007622 A2 BR 112022007622A2 BR 112022007622 A BR112022007622 A BR 112022007622A BR 112022007622 A BR112022007622 A BR 112022007622A BR 112022007622 A2 BR112022007622 A2 BR 112022007622A2
Authority
BR
Brazil
Prior art keywords
electroporation
systems
cartridges
devices
methods
Prior art date
Application number
BR112022007622A
Other languages
English (en)
Portuguese (pt)
Inventor
Huei Yeo Steven
Wee Hon Siu
Wei Han
Peck Yvonne
Xuang Lee Way
Shi Benyong
Wai Chan Chee
Heng Lim Beng
Shanmugam Dhanaraj
Mei Lin Teo Stacey
Yan Tham Jun
Shyong Chong Kok
Yang Lim Li
Gordon Michael
Andronikou Nektaria
De Mollerat Du Jeu Xavier
Rossmann Chris
Forke Ulrich
Original Assignee
Life Technologies Corp
Life Tech Holdings Pte Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp, Life Tech Holdings Pte Limited filed Critical Life Technologies Corp
Publication of BR112022007622A2 publication Critical patent/BR112022007622A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
BR112022007622A 2019-10-25 2020-10-23 Sistemas, métodos e dispositivos para eletroporação de fluido contendo células BR112022007622A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962926078P 2019-10-25 2019-10-25
PCT/US2020/057138 WO2021081381A1 (en) 2019-10-25 2020-10-23 Systems and devices for electroporation of cell-containing fluid

Publications (1)

Publication Number Publication Date
BR112022007622A2 true BR112022007622A2 (pt) 2022-10-11

Family

ID=73402180

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022007622A BR112022007622A2 (pt) 2019-10-25 2020-10-23 Sistemas, métodos e dispositivos para eletroporação de fluido contendo células

Country Status (10)

Country Link
US (2) US20210123009A1 (https=)
EP (1) EP4048776A1 (https=)
JP (2) JP7784374B2 (https=)
KR (1) KR20220088469A (https=)
CN (2) CN214361426U (https=)
AU (1) AU2020370481B2 (https=)
BR (1) BR112022007622A2 (https=)
CA (1) CA3154506A1 (https=)
DE (1) DE202020106067U1 (https=)
WO (1) WO2021081381A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12097345B2 (en) * 2018-06-01 2024-09-24 Penumbra, Inc. Infusion catheter and methods of use
EP4048776A1 (en) * 2019-10-25 2022-08-31 Life Technologies Corporation Systems and devices for electroporation of cell-containing fluid
USD943760S1 (en) * 2020-05-11 2022-02-15 Maxcyte, Inc. Electroporation processing assembly
USD944410S1 (en) 2020-05-11 2022-02-22 Maxcyte, Inc. Electroporation processing assembly
USD965170S1 (en) * 2020-10-23 2022-09-27 Life Technologies Corporation Electroporation device
US20230110090A1 (en) * 2021-10-08 2023-04-13 Life Technologies Holdings Pte Limited System and Method of Electroporation Protocol Optimization
TW202400835A (zh) 2022-03-09 2024-01-01 荷蘭商Ａｓｍ Ｉｐ私人控股有限公司 反應器系統、控制液體源的溫度之方法、及經調適以容納容器的裝置
WO2023230253A1 (en) * 2022-05-27 2023-11-30 Sqz Biotechnologies Company Cartridges and devices for use in a system for delivery of a payload into a cell
CN117431158A (zh) * 2022-07-14 2024-01-23 苏州壹达生物科技有限公司 一种流式电穿孔装置
US20260092248A1 (en) 2022-09-15 2026-04-02 Life Technologies Corporation Bioreactors operable in static and dynamic modes and methods of use
KR102609307B1 (ko) * 2022-10-24 2023-12-04 티큐브잇(주) 유전자 형질 감염 장치
CN115722292B (zh) * 2022-11-22 2023-12-29 山东省煤田地质局第一勘探队 一种地下水自然资源调查用分类收集装置及使用方法
CN120882852A (zh) * 2023-06-21 2025-10-31 隆萨科隆有限公司 多腔室电穿孔盒及其使用方法
WO2026043806A2 (en) 2024-08-19 2026-02-26 Life Technologies Corporation Systems, methods, and assemblies for cell processing
CN120028438B (zh) * 2025-04-23 2025-08-26 晋城市鑫环球铸造有限公司 一种基于球墨铸铁管生产的探伤检测设备及方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5650305A (en) * 1996-05-15 1997-07-22 Health Research Inc. Method to produce hybrid cells by electrofusion
US6150148A (en) 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
US6969604B1 (en) * 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US20050282283A1 (en) * 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
DK1766057T3 (en) * 2004-05-12 2015-03-02 Maxcyte Inc Methods and devices related to a regulated flowelektroporationskammer
WO2006112870A1 (en) * 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
WO2011050009A1 (en) * 2009-10-19 2011-04-28 Rational Biotechnology Inc. Method, device and apparatus for inducing self-adjusting cell electroporation
US9382510B2 (en) 2011-08-25 2016-07-05 Jian Chen Methods and devices for electroporation
US9204833B2 (en) * 2012-03-27 2015-12-08 Phlebotics, Inc. Cartridge for automated blood sampling system
US8766641B2 (en) * 2012-08-28 2014-07-01 Halliburton Energy Services, Inc. Determining surface wetting of rock with changing well fluids
CN104403943B (zh) * 2014-12-02 2016-06-15 陈剑 一种电击管和具有电击管的细胞电转仪
WO2016088062A1 (zh) * 2014-12-02 2016-06-09 陈剑 一种电击管和具有电击管的细胞电转仪
CA3017108A1 (en) * 2015-03-12 2016-09-15 The Trustees Of The University Of Pennsylvania System, method, and device for high-throughput, automated culturing of genetically modified organisms
JP6660057B2 (ja) 2015-12-11 2020-03-04 国立大学法人豊橋技術科学大学 エレクトロポレーション用電極及び外来物質導入装置
MX2019015505A (es) * 2017-06-30 2020-07-28 Inscripta Inc Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados.
CN109679844B (zh) 2017-10-19 2023-08-22 苏州壹达生物科技有限公司 一种流式电穿孔装置
JP2021506309A (ja) * 2017-12-20 2021-02-22 スクイーズ バイオテクノロジーズ カンパニー 細胞の中へのペイロードの送達のためのシステム
US20190225928A1 (en) * 2018-01-22 2019-07-25 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems comprising filtration devices
EP4048776A1 (en) * 2019-10-25 2022-08-31 Life Technologies Corporation Systems and devices for electroporation of cell-containing fluid

Also Published As

Publication number Publication date
AU2020370481A1 (en) 2022-05-26
WO2021081381A1 (en) 2021-04-29
JP2026040486A (ja) 2026-03-09
CA3154506A1 (en) 2021-04-29
US20210123009A1 (en) 2021-04-29
EP4048776A1 (en) 2022-08-31
AU2020370481B2 (en) 2026-04-02
CN214361426U (zh) 2021-10-08
KR20220088469A (ko) 2022-06-27
JP2023501142A (ja) 2023-01-18
DE202020106067U1 (de) 2020-12-04
JP7784374B2 (ja) 2025-12-11
US20240263124A1 (en) 2024-08-08
CN112708552A (zh) 2021-04-27

Similar Documents

Publication Publication Date Title
BR112022007622A2 (pt) Sistemas, métodos e dispositivos para eletroporação de fluido contendo células
MX2017006557A (es) Aparato y metodo para medir la concentracion de desinfectante en un sistema de reprocesamiento de dispositivos medicos.
BR112014032923A2 (pt) instrumentos cirúrgicos com sistema de gerenciamento de fluido
BR112014000965A2 (pt) método, e aparelho
BR112012026366A2 (pt) sistemas e dispositivos para análise de amostras
BR112012013813A2 (pt) "aparelho e método para processamento de amostras biológicas".
BR112015012611B8 (pt) módulo de troca de calor para uso em um sistema de reator químico, farmacêutico ou biológico
BR112014017188A8 (pt) células hospedeiras com endossimbiontes artificiais
Astuti et al. Pemilihan Metode Intensitas Hujan yang Sesuai dengan Stasiun Hujan Pekanbaru
BR112016015394A8 (pt) sistemas de geração de pulso elétrico
BR112012020982A2 (pt) Estação de transferência para amostragem de material vegetal e sistema de rastreamento
BR112015009466A2 (pt) cartucho de medicamento líquido e métodos para tamponar solução de medicamento carregada em recipiente e medicamento líquido tendo tonicidade e ph alvos
EA202192289A1 (ru) Способы мечения эукариотных клеток многоклеточного организма, а также лечения и/или диагностики рака с использованием модифицированных моносахаридных соединений
BR112014017081A2 (pt) sistema de recuperação e preservação de amostra de tecido; sistema de preservação de tecido; e sistema de resfriamento
BR112012011952A2 (pt) conjunto de destilador solar
BR112016018797A2 (pt) Aperfeiçoamento de expressão de proteína recombinante com o uso de um sistema de retenção de célula baseado em membrana
MX2021007342A (es) Indicador biologico para sistema de esterilizacion quimico-liquido.
Deelder et al. Response to: accuracy of circulating cathodic antigen tests for rapid mapping of Schistosoma mansoni and S. haematobium infections in Southern Sudan by RA Ashton et al.(2011) Trop Med Int Health 16, pp. 1099-1103
BR112016029371A2 (pt) tira de teste analítico de base eletroquímica com câmaras receptoras de amostra cruzadas
BR112018068057A2 (pt) pluralidade de partículas de dispensação de fármaco sensíveis a ph, composição, frasco, dispositivo para administração parenteral, uso de uma pluralidade de partículas ou de uma composição, métodos para obter uma resposta imune, de tratamento ou profilaxia, para impedir ou reduzir a interação entre uma carga biologicamente ativa e componentes de um ambiente aquoso, para produzir uma pluralidade de partículas de dispensação de fármaco e para produzir uma composição, e, vacina
Walther et al. Microgravity cell culture systems and bioreactors: current status and future developments
AR117115A1 (es) Métodos para la conservación de reactivo de glóbulos rojos usando monóxido de carbono
Zhang et al. Histone ubiquitylation and its roles in DNA damage response
Antyushev «SOCIAL ORGANISM»: FROM THE ORIGIN TO THE PRESENT
Thenankal A Study on Futures Trading in Commodities With Special Reference to Rubber

Legal Events

Date Code Title Description
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements