BR112017025961A2 - gene wms de macho- esterilidade de trigo e seu promotor de expressão específico de antera e usos dos mesmos - Google Patents

gene wms de macho- esterilidade de trigo e seu promotor de expressão específico de antera e usos dos mesmos

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Publication number
BR112017025961A2
BR112017025961A2 BR112017025961A BR112017025961A BR112017025961A2 BR 112017025961 A2 BR112017025961 A2 BR 112017025961A2 BR 112017025961 A BR112017025961 A BR 112017025961A BR 112017025961 A BR112017025961 A BR 112017025961A BR 112017025961 A2 BR112017025961 A2 BR 112017025961A2
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BR
Brazil
Prior art keywords
anther
gene
wms
wheat
specific
Prior art date
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BR112017025961A
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English (en)
Other versions
BR112017025961B1 (pt
Inventor
Lv Bo
Fu Daolin
Ni Fei
Qi Juan
Luo Mincheng
Wang Shuyun
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Univ Shandong Agricultural
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Application filed by Univ Shandong Agricultural filed Critical Univ Shandong Agricultural
Publication of BR112017025961A2 publication Critical patent/BR112017025961A2/pt
Publication of BR112017025961B1 publication Critical patent/BR112017025961B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

a presente invenção proporciona um novo gene wms que confere macho-esterilidade de trigo, o seu promotor de expressão específico de antera e usos dos mesmos. no trigo, um gene ms2 bem conhecido que causa macho-esterilidade dominante tem sido amplamente aplicado na seleção recorrente na china. uma abordagem de rna-seq foi realizada para revelar o transcritoma específico de antera em um par de linhas isogênicas de ms2, ?lumai 15? e ?lumai 15 + ms2?. como resultado, um gene wms foi identificado mostrando expressão específica de antera no estágio inicial da meiose e apenas em trigo carregando o gene ms2. a regulação do wms poderia alterar a macho-fertilidade da planta. verificou-se que o promotor de wms compreende uma atividade específica de antera. assim, a presente invenção pode ser usada para alcançar a expressão de genes específicos da antera, para desenvolver macho-esterilidade em várias espécies de plantas, para estabelecer a seleção recorrente em várias espécies de plantas e para auxiliar a produção de sementes híbridas.
BR112017025961-3A 2015-06-04 2016-04-11 Vetor compreendendo dna isolado e promotor de expressão específico de antera BR112017025961B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201510303817.0A CN104911194B (zh) 2015-06-04 2015-06-04 小麦雄性不育基因wms及其花药特异性启动子的应用
CN201510303817.0 2015-06-04
PCT/IB2016/000537 WO2016193798A1 (en) 2015-06-04 2016-04-11 Wheat male-sterility gene wms and its anther-specific expression promoter and uses thereof

Publications (2)

Publication Number Publication Date
BR112017025961A2 true BR112017025961A2 (pt) 2018-08-14
BR112017025961B1 BR112017025961B1 (pt) 2023-12-05

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Application Number Title Priority Date Filing Date
BR112017025961-3A BR112017025961B1 (pt) 2015-06-04 2016-04-11 Vetor compreendendo dna isolado e promotor de expressão específico de antera

Country Status (13)

Country Link
US (2) US11124797B1 (pt)
EP (1) EP3303592B1 (pt)
JP (1) JP6899779B2 (pt)
CN (2) CN109402141B (pt)
AU (2) AU2016272688B2 (pt)
BR (1) BR112017025961B1 (pt)
CA (1) CA2988040C (pt)
DK (1) DK3303592T3 (pt)
EA (1) EA201792344A1 (pt)
LT (1) LT3303592T (pt)
MX (1) MX2017013699A (pt)
UA (1) UA125682C2 (pt)
WO (1) WO2016193798A1 (pt)

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US9803214B2 (en) * 2013-03-12 2017-10-31 Pioneer Hi-Bred International, Inc. Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility
CN109402141B (zh) * 2015-06-04 2021-10-01 山东农业大学 小麦雄性不育基因wms及其花药特异性启动子的应用
CN106800594A (zh) * 2015-11-26 2017-06-06 中国农业科学院作物科学研究所 小麦太谷核不育基因ms2及其应用
CN106446597B (zh) * 2016-09-06 2018-11-23 清华大学 多物种特征选择及鉴定未知基因的方法
EP3601578A1 (en) * 2017-03-23 2020-02-05 Basf Se Anther-specific promoter and uses thereof
CN107974450A (zh) * 2017-12-31 2018-05-01 青岛袁策生物科技有限公司 一种无碱基突变长序列基因的pcr高效扩增方法
EP4061118A4 (en) * 2019-11-21 2024-09-04 Northern Agri Brands Llc TRITICALE CULTIVAR 343CMS AND NEW SEQUENCES FOR MALE STERILITY
CN113430209B (zh) * 2020-03-05 2022-09-20 山东农业大学 大麦雄性不育基因bms-1及其应用
CN112779269A (zh) * 2021-02-09 2021-05-11 南京农业大学 一个新疆稻麦子促进颖壳增长基因p1及其编码的蛋白
PL439284A1 (pl) * 2021-10-22 2023-04-24 Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Syntetyczny regulator ekspresji genów oraz sposób wytwarzania mieszańców roślin uprawnych z jego wykorzystaniem
CN116312796B (zh) * 2022-12-27 2023-11-14 江苏先声医学诊断有限公司 一种基于期望最大化算法的宏基因组丰度估计方法及系统

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CN109402141B (zh) * 2015-06-04 2021-10-01 山东农业大学 小麦雄性不育基因wms及其花药特异性启动子的应用
EP3601578A1 (en) * 2017-03-23 2020-02-05 Basf Se Anther-specific promoter and uses thereof

Also Published As

Publication number Publication date
CN109402141A (zh) 2019-03-01
BR112017025961B1 (pt) 2023-12-05
US20220073938A1 (en) 2022-03-10
WO2016193798A1 (en) 2016-12-08
JP6899779B2 (ja) 2021-07-07
CA2988040A1 (en) 2016-12-08
LT3303592T (lt) 2022-06-10
DK3303592T3 (da) 2022-05-30
US11124797B1 (en) 2021-09-21
EA201792344A1 (ru) 2018-08-31
CA2988040C (en) 2023-09-26
AU2022201656B2 (en) 2024-05-02
AU2016272688B2 (en) 2021-12-09
US11795466B2 (en) 2023-10-24
JP2018521630A (ja) 2018-08-09
CN104911194B (zh) 2019-03-08
EP3303592A4 (en) 2018-10-24
CN109402141B (zh) 2021-10-01
EP3303592B1 (en) 2022-02-23
MX2017013699A (es) 2018-08-01
UA125682C2 (uk) 2022-05-18
CN104911194A (zh) 2015-09-16
AU2016272688A1 (en) 2017-10-19
AU2022201656A1 (en) 2022-03-31
EP3303592A1 (en) 2018-04-11

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