BR0316078A - Métodos automáticos de isolamento e quantificação de ácido nucléico de alta passagem - Google Patents

Métodos automáticos de isolamento e quantificação de ácido nucléico de alta passagem

Info

Publication number
BR0316078A
BR0316078A BR0316078-5A BR0316078A BR0316078A BR 0316078 A BR0316078 A BR 0316078A BR 0316078 A BR0316078 A BR 0316078A BR 0316078 A BR0316078 A BR 0316078A
Authority
BR
Brazil
Prior art keywords
rna
nucleic acid
quantification
extracting
isolation
Prior art date
Application number
BR0316078-5A
Other languages
English (en)
Inventor
Diana R Mcwilliams
Robert G Becker
Charles W Bolten
Pamela De Ciechi
Shawn D Miller
Andrew T Pinz
Jeffrey V Surry
Original Assignee
Pharmacia Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Corp filed Critical Pharmacia Corp
Publication of BR0316078A publication Critical patent/BR0316078A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

"MéTODOS AUTOMáTICOS DE ISOLAMENTO E QUANTIFICAçãO DE áCIDO NUCLéICO DE ALTA PASSAGEM". Um protocolo de laboratório de RNA de alta velocidade de passagem é provido para extração e manutenção de uma quantidade suficiente de RNA de alta qualidade durante a preparação da amostra de modo a analisar vários genes de uma vez com a assistência de análise por computador. A invenção exposta inclui um método para analisar o RNA compreendendo as etapas de extrair o RNA de um constructo biológico complexo em quantidades suficientes para prover dados de RNA precisos, transferir o RNA para um aparelho que mantém o RNA e reagentes necessários em uma temperatura entre cerca de 0 a 10 <198>C e analisar o RNA com uma análise matemática gerada por computador dos dados para acessar a presença do RNA e finalmente testar a eficácia de uma droga.
BR0316078-5A 2002-11-08 2003-11-07 Métodos automáticos de isolamento e quantificação de ácido nucléico de alta passagem BR0316078A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42513902P 2002-11-08 2002-11-08
US42513602P 2002-11-08 2002-11-08
PCT/US2003/035686 WO2004044221A2 (en) 2002-11-08 2003-11-07 High throughput automatic nucleic acid isolation and quantitation methods

Publications (1)

Publication Number Publication Date
BR0316078A true BR0316078A (pt) 2005-09-27

Family

ID=32314573

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0316078-5A BR0316078A (pt) 2002-11-08 2003-11-07 Métodos automáticos de isolamento e quantificação de ácido nucléico de alta passagem

Country Status (8)

Country Link
US (1) US20040115720A1 (pt)
EP (1) EP1558765A4 (pt)
JP (1) JP2006505283A (pt)
AU (1) AU2003287603A1 (pt)
BR (1) BR0316078A (pt)
CA (1) CA2502822A1 (pt)
MX (1) MXPA05004970A (pt)
WO (1) WO2004044221A2 (pt)

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Also Published As

Publication number Publication date
EP1558765A2 (en) 2005-08-03
US20040115720A1 (en) 2004-06-17
AU2003287603A8 (en) 2004-06-03
EP1558765A4 (en) 2005-11-30
WO2004044221A3 (en) 2004-07-15
JP2006505283A (ja) 2006-02-16
MXPA05004970A (es) 2005-08-02
CA2502822A1 (en) 2004-05-27
AU2003287603A1 (en) 2004-06-03
WO2004044221A2 (en) 2004-05-27

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]