BR0114928A - Métodos de detecção quantitativa para determinar quantitativamente uma relação de teor de um recombinante genético e uma relação de teor individual de uma linha de recombinante genético individual em uma amostra contendo pelo menos uma linha de recombinante genético, e, molécula de dna recombinante - Google Patents

Métodos de detecção quantitativa para determinar quantitativamente uma relação de teor de um recombinante genético e uma relação de teor individual de uma linha de recombinante genético individual em uma amostra contendo pelo menos uma linha de recombinante genético, e, molécula de dna recombinante

Info

Publication number
BR0114928A
BR0114928A BR0114928-8A BR0114928A BR0114928A BR 0114928 A BR0114928 A BR 0114928A BR 0114928 A BR0114928 A BR 0114928A BR 0114928 A BR0114928 A BR 0114928A
Authority
BR
Brazil
Prior art keywords
recombinant
content ratio
genetic
genetic recombinant
individual
Prior art date
Application number
BR0114928-8A
Other languages
English (en)
Inventor
Akihiro Hino
Takeshi Matsuoka
Hideo Kuribara
Tomoaki Yoshimura
Yoichiro Shindo
Satoshi Futo
Machiko Ogawa
Original Assignee
Nat Food Res
Asahi Breweries Ltd
Nippon Flour Mills
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Food Res, Asahi Breweries Ltd, Nippon Flour Mills filed Critical Nat Food Res
Publication of BR0114928A publication Critical patent/BR0114928A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Compounds That Contain Two Or More Ring Oxygen Atoms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

"MéTODOS DE DETECçãO QUANTITATIVA PARA DETERMINAR QUANTITATIVAMENTE UMA RELAçãO DE TEOR DE UM RECOMBINANTE GENéTICO E UMA RELAçãO DE TEOR INDIVIDUAL DE UMA LINHA DE RECOMBINANTE GENéTICO INDIVIDUAL EM UMA AMOSTRA CONTENDO PELO MENOS UMA LINHA DE RECOMBINANTE GENéTICO, E, MOLéCULA DE DNA RECOMBINANTE". Um método de detectar um recombinante genético usando-se o método da PCR. Isto é, um método de detectar quantitativamente uma modificação genética por meio da qual a relação das modificações genéticas totais em uma massa contendo um número múltiplo de cepas geneticamente modificadas e as relações individuais das cepas na massa podem ser acuradamente quantificadas. Este método compreende usar, como uma molécula padrão, uma molécula tendo uma seq³ência de DNA específica para uma modificação genética e uma molécula de DNA intrínseca comum às espécie biológicas que correspondem à modificação acima na molécula única, submetendo uma amostra que potencialmente contenha a modificação para a PCR quantitativa, respectivamente para a seq³ência de DNA específica e a seq³ência de DNA intrínseca como descrito acima e depois determinar a relação das moléculas existentes.
BR0114928-8A 2000-10-26 2001-10-24 Métodos de detecção quantitativa para determinar quantitativamente uma relação de teor de um recombinante genético e uma relação de teor individual de uma linha de recombinante genético individual em uma amostra contendo pelo menos uma linha de recombinante genético, e, molécula de dna recombinante BR0114928A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000326738 2000-10-26
PCT/JP2001/009344 WO2002034943A1 (fr) 2000-10-26 2001-10-24 Procede de quantification de modification genetique et molecule standard a utiliser dans ledit procede

Publications (1)

Publication Number Publication Date
BR0114928A true BR0114928A (pt) 2004-02-03

Family

ID=18803876

Family Applications (1)

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BR0114928-8A BR0114928A (pt) 2000-10-26 2001-10-24 Métodos de detecção quantitativa para determinar quantitativamente uma relação de teor de um recombinante genético e uma relação de teor individual de uma linha de recombinante genético individual em uma amostra contendo pelo menos uma linha de recombinante genético, e, molécula de dna recombinante

Country Status (13)

Country Link
US (1) US7709232B2 (pt)
EP (1) EP1335027B1 (pt)
JP (1) JP4291568B2 (pt)
KR (1) KR100624667B1 (pt)
CN (1) CN100422341C (pt)
AT (1) ATE396281T1 (pt)
AU (2) AU2002212678B2 (pt)
BR (1) BR0114928A (pt)
CA (1) CA2427126C (pt)
DE (1) DE60134168D1 (pt)
HK (1) HK1076487A1 (pt)
MX (1) MXPA03003731A (pt)
WO (1) WO2002034943A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080064028A1 (en) * 2003-05-16 2008-03-13 Takashi Hirao Quantitative Pcr Method of Detecting Specific Plant Genus in Food or Food Ingredient
KR100664992B1 (ko) 2005-03-21 2007-01-04 대한민국 유전자 변형체의 검정을 위한 프라이머 세트，검정방법 및이에 사용되는 표준 플라스미드
JP4925607B2 (ja) * 2005-05-11 2012-05-09 国立医薬品食品衛生研究所長 遺伝子組換え加工食品の定量的検知方法
CN102424863B (zh) * 2012-01-06 2013-06-12 海康生物科技（北京）有限公司 一种增强型荧光定量pcr方法
CN102586304B (zh) * 2012-01-12 2013-11-13 中国计量学院 含多种转座因子的抗虫水稻、棉花标准质粒及其应用
CN102676674A (zh) * 2012-05-10 2012-09-19 海康生物科技（北京）有限公司 一种转基因玉米检测用质粒标准分子及其制备方法
JP6093934B2 (ja) * 2012-08-10 2017-03-15 国立研究開発法人農業・食品産業技術総合研究機構 目的の塩基配列を有するｄｎａを１分子含む試料の調製方法
WO2019063795A1 (en) * 2017-09-30 2019-04-04 Bioatlas Oü MULTI-COMPONENT TABLET FOR THE PRODUCTION OF AGAROSE GEL AND RELATED METHODS
JP7386655B2 (ja) * 2019-09-13 2023-11-27 国立研究開発法人農業・食品産業技術総合研究機構 遺伝子組換え体の検出方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
JP4306957B2 (ja) * 1997-09-12 2009-08-05 ピーエイチアールアイ プロパティーズ，インコーポレイテッド 非競合的共増幅法
EP1358346A2 (en) * 2000-03-07 2003-11-05 E.I. Dupont De Nemours And Company Real time quantitative pcr with intercalating dye for single and multiplex target dna
US7122645B2 (en) * 2000-03-27 2006-10-17 Smithkline Beecham Corporation Detection of viable agents

Also Published As

Publication number Publication date
DE60134168D1 (de) 2008-07-03
CN100422341C (zh) 2008-10-01
US20040005605A1 (en) 2004-01-08
AU2002212678B8 (en) 2002-05-06
JP4291568B2 (ja) 2009-07-08
CN1623002A (zh) 2005-06-01
AU2002212678B2 (en) 2007-03-15
WO2002034943A1 (fr) 2002-05-02
AU1267802A (en) 2002-05-06
CA2427126A1 (en) 2003-05-12
EP1335027B1 (en) 2008-05-21
CA2427126C (en) 2009-05-26
EP1335027A1 (en) 2003-08-13
EP1335027A4 (en) 2004-08-04
KR100624667B1 (ko) 2006-09-18
HK1076487A1 (en) 2006-01-20
ATE396281T1 (de) 2008-06-15
JPWO2002034943A1 (ja) 2004-03-04
MXPA03003731A (es) 2004-10-15
KR20030051740A (ko) 2003-06-25
US7709232B2 (en) 2010-05-04

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B25D Requested change of name of applicant approved

Owner name: NATIONAL FOOD RESEARCH INSTITUTE (JP) , NIPPON FLO

Free format text: NOME ALTERADO DE: ASAHI BREWERIES, LTD.

B25G Requested change of headquarter approved

Owner name: NATIONAL FOOD RESEARCH INSTITUTE (JP) , NIPPON FLO

Free format text: ENDERECO ALTERADO CONFORME SOLICITADO NA PETICAO NO 020110128249/RJ DE 13/12/2011.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O COMBINADO COM ART. 13, ART. 10 E ART. 25 DA LPI

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.