AU594503B2 - Immunoassay reagents, kits and methods - Google Patents
Immunoassay reagents, kits and methods Download PDFInfo
- Publication number
- AU594503B2 AU594503B2 AU78623/87A AU7862387A AU594503B2 AU 594503 B2 AU594503 B2 AU 594503B2 AU 78623/87 A AU78623/87 A AU 78623/87A AU 7862387 A AU7862387 A AU 7862387A AU 594503 B2 AU594503 B2 AU 594503B2
- Authority
- AU
- Australia
- Prior art keywords
- cells
- enzyme
- antigen
- antibody
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims description 38
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 33
- 238000003018 immunoassay Methods 0.000 title claims description 25
- 102000004190 Enzymes Human genes 0.000 claims description 56
- 108090000790 Enzymes Proteins 0.000 claims description 56
- 239000000427 antigen Substances 0.000 claims description 33
- 102000036639 antigens Human genes 0.000 claims description 33
- 108091007433 antigens Proteins 0.000 claims description 33
- 108090000623 proteins and genes Proteins 0.000 claims description 30
- 239000003550 marker Substances 0.000 claims description 25
- 238000012360 testing method Methods 0.000 claims description 23
- 238000009739 binding Methods 0.000 claims description 22
- 230000027455 binding Effects 0.000 claims description 21
- 239000007787 solid Substances 0.000 claims description 21
- 108060003951 Immunoglobulin Proteins 0.000 claims description 20
- 102000018358 immunoglobulin Human genes 0.000 claims description 20
- 239000000725 suspension Substances 0.000 claims description 17
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 230000000694 effects Effects 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 13
- 239000007900 aqueous suspension Substances 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 12
- 241000191967 Staphylococcus aureus Species 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 10
- 241000191940 Staphylococcus Species 0.000 claims description 9
- 102000016938 Catalase Human genes 0.000 claims description 7
- 108010053835 Catalase Proteins 0.000 claims description 7
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 7
- 238000011065 in-situ storage Methods 0.000 claims description 6
- 230000000717 retained effect Effects 0.000 claims description 6
- 238000000159 protein binding assay Methods 0.000 claims description 5
- 241000194017 Streptococcus Species 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 230000009870 specific binding Effects 0.000 claims description 4
- 239000000969 carrier Substances 0.000 claims description 3
- 238000011160 research Methods 0.000 claims description 3
- 108010006464 Hemolysin Proteins Proteins 0.000 claims description 2
- 239000002250 absorbent Substances 0.000 claims description 2
- 230000002745 absorbent Effects 0.000 claims description 2
- 238000011161 development Methods 0.000 claims description 2
- 239000003228 hemolysin Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 230000009083 hemolysis by symbiont of host erythrocytes Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 55
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 20
- 239000002953 phosphate buffered saline Substances 0.000 description 20
- 102000004169 proteins and genes Human genes 0.000 description 17
- 238000003556 assay Methods 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
- 101710120037 Toxin CcdB Proteins 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
- 229940072221 immunoglobulins Drugs 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 3
- 101100026203 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) neg-1 gene Proteins 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000001974 tryptic soy broth Substances 0.000 description 3
- OPEGYZAATHKDEM-HCWXCVPCSA-N (2r,4s)-2-[(r)-carboxy(formamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid Chemical compound CC1(C)S[C@H]([C@H](NC=O)C(O)=O)N[C@H]1C(O)=O OPEGYZAATHKDEM-HCWXCVPCSA-N 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 108091008324 binding proteins Proteins 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 239000000700 radioactive tracer Substances 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 241000589562 Brucella Species 0.000 description 1
- 241000589567 Brucella abortus Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 238000009007 Diagnostic Kit Methods 0.000 description 1
- YCAGGFXSFQFVQL-UHFFFAOYSA-N Endothion Chemical compound COC1=COC(CSP(=O)(OC)OC)=CC1=O YCAGGFXSFQFVQL-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 108010087702 Penicillinase Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 241000295644 Staphylococcaceae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108700011201 Streptococcus IgG Fc-binding Proteins 0.000 description 1
- 241000193996 Streptococcus pyogenes Species 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 229940056450 brucella abortus Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 239000008098 formaldehyde solution Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 231100000206 health hazard Toxicity 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229940027941 immunoglobulin g Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- XMBWDFGMSWQBCA-RNFDNDRNSA-M iodine-131(1-) Chemical compound [131I-] XMBWDFGMSWQBCA-RNFDNDRNSA-M 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229950009506 penicillinase Drugs 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000011272 standard treatment Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- -1 test tubes Substances 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56938—Staphylococcus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL80083A IL80083A0 (en) | 1986-09-19 | 1986-09-19 | Immunoassay reagents,kits and methods |
| IL80083 | 1986-09-19 | ||
| IL80313 | 1986-10-15 | ||
| IL80313A IL80313A0 (en) | 1986-09-19 | 1986-10-15 | Immunoassay reagents,kits and methods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU7862387A AU7862387A (en) | 1988-03-24 |
| AU594503B2 true AU594503B2 (en) | 1990-03-08 |
Family
ID=26321590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU78623/87A Ceased AU594503B2 (en) | 1986-09-19 | 1987-09-17 | Immunoassay reagents, kits and methods |
Country Status (9)
| Country | Link |
|---|---|
| AU (1) | AU594503B2 (enrdf_load_stackoverflow) |
| CA (1) | CA1294871C (enrdf_load_stackoverflow) |
| CH (1) | CH675309A5 (enrdf_load_stackoverflow) |
| DE (1) | DE3731227A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2604259B1 (enrdf_load_stackoverflow) |
| GB (1) | GB2197468B (enrdf_load_stackoverflow) |
| IL (1) | IL80313A0 (enrdf_load_stackoverflow) |
| IT (1) | IT1223303B (enrdf_load_stackoverflow) |
| NL (1) | NL8702235A (enrdf_load_stackoverflow) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989010974A1 (en) * | 1988-05-11 | 1989-11-16 | Trustees Of The Sisters Of Charity Of Australia | Enzyme immunoassay system |
| US5759774A (en) * | 1988-05-18 | 1998-06-02 | Cobe Laboratories, Inc. | Method of detecting circulating antibody types using dried or lyophilized cells |
| DE3936256A1 (de) * | 1989-10-31 | 1991-05-02 | Max Planck Gesellschaft | Test zur differentiellen quantifizierung des freien bzw. des peptidasekomplexierten proteinaseinhibitors (alpha)(pfeil abwaerts)2(pfeil abwaerts)-makroglobulin |
| WO2008136861A2 (en) * | 2006-11-22 | 2008-11-13 | 3M Innovative Properties Company | Inactivated and dried biological preparations |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3990947A (en) * | 1975-03-07 | 1976-11-09 | Warner-Lambert Company | Composition for detecting fibrinogen, fibrinogen split products and fibrin split products |
| FR2405301A1 (fr) * | 1977-10-04 | 1979-05-04 | Api Labor | Substrats et procede permettant l'identification rapide de bacteries du genre streptococcus |
| SE7712244L (sv) * | 1977-10-31 | 1979-05-01 | Jonsson U R S | Forfarande for framstellning av preparationer av avdodade bakterier |
| US4471058A (en) * | 1982-07-26 | 1984-09-11 | Board Of Trustees Operating Michigan State University | Method for the detection and/or determination of a polyvalent antigen using at least two different monoclonal antibodies |
-
1986
- 1986-10-15 IL IL80313A patent/IL80313A0/xx unknown
-
1987
- 1987-09-10 GB GB8721276A patent/GB2197468B/en not_active Expired - Lifetime
- 1987-09-16 CH CH3575/87A patent/CH675309A5/fr not_active IP Right Cessation
- 1987-09-17 AU AU78623/87A patent/AU594503B2/en not_active Ceased
- 1987-09-17 IT IT21950/87A patent/IT1223303B/it active
- 1987-09-17 DE DE19873731227 patent/DE3731227A1/de not_active Withdrawn
- 1987-09-17 FR FR878712904A patent/FR2604259B1/fr not_active Expired - Lifetime
- 1987-09-18 CA CA000547275A patent/CA1294871C/en not_active Expired - Lifetime
- 1987-09-18 NL NL8702235A patent/NL8702235A/nl not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| AU7862387A (en) | 1988-03-24 |
| NL8702235A (nl) | 1988-04-18 |
| FR2604259A1 (fr) | 1988-03-25 |
| GB2197468A (en) | 1988-05-18 |
| IT1223303B (it) | 1990-09-19 |
| CH675309A5 (enrdf_load_stackoverflow) | 1990-09-14 |
| FR2604259B1 (fr) | 1991-05-10 |
| GB2197468B (en) | 1991-03-13 |
| DE3731227A1 (de) | 1988-06-30 |
| GB8721276D0 (en) | 1987-10-14 |
| IT8721950A0 (it) | 1987-09-17 |
| IL80313A0 (en) | 1987-01-30 |
| CA1294871C (en) | 1992-01-28 |
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