AU2021104128A4 - Measuring method and device of chemiluminescence detector - Google Patents
Measuring method and device of chemiluminescence detector Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/124—Sensitivity
- G01N2201/1245—Averaging several measurements
Abstract
The invention disclose a measuring method and device of a chemiluminescence detector. The
method comprises the follow steps: controlling a light sensor to sequentially collect the
luminescence of samples in each measured channel according to preset rules, and respectively
outputting luminescence signal values representing the luminescence intensity of samples in
the measured channels; The chemiluminescence detector comprises a plurality of measured
channels, and samples are respectively placed in each measured channel; Using an algorithm
matched with a preset rule to calculate the calculated value of the luminous signal of each
measured channel according to the luminous signal value data corresponding to each measured
channel; According to the calculated value of luminous signal of each measured channel and
the relationship between the specified analyte concentration value and the calculated value of
luminous signal, the specified analyte concentration value of samples in each measured channel
is calculated. According to the method and the device, a chemiluminescence detector can
measure a plurality of samples to obtain inspection results by using one light sensor, thereby
saving the cost of the device and improving the competitiveness of products.
1/1
FIGURES
Controlling a light sensor to sequentially collect the
luminescence of samples in each measured channel
according to preset rules
Using an algorithm matched with a preset rule to calculate S1 I
the calculated value of the luminous signal of each
measured channel according to the luminous signal value
data corresponding to each measured channel
According to the calculated value of luminous signal of each
measured channel and the relationship between the specified
analyte concentration value and the calculated value of
luminous signal, the specified analyte concentration value of
samples in each measured channel is calculated
Description
1/1
Controlling a light sensor to sequentially collect the luminescence of samples in each measured channel according to preset rules
Using an algorithm matched with a preset rule to calculate S1 I the calculated value of the luminous signal of each measured channel according to the luminous signal value data corresponding to each measured channel
According to the calculated value of luminous signal of each measured channel and the relationship between the specified analyte concentration value and the calculated value of luminous signal, the specified analyte concentration value of samples in each measured channel is calculated
Measuring method and device of chemiluminescence detector
The invention relate to that technical field of chemiluminescence device, in particular to a
measuring method and a measuring device of a chemiluminescence detector.
In recent years, chemiluminescence biological detection detector have been widely used
in the field of medical devices because of their advantages of high sensitivity, wide linear
range and good stability.
In addition to the large-scale chemiluminescence detector commonly used in hospital
inspection departments, there are also small-scale point-of-care testing
chemiluminescence detector.. Compared with large-scale detector, point-of-care testing
chemiluminescence detector can directly test at the sampling place without sending the
samples to the inspection department, and can get the results quickly. The point-of-care
testing chemiluminescence detector is small in size and can be placed on the desktop. It
can be conveniently applied to emergency departments of hospital, special specialized
hospitals, maternal and child health hospitals, community hospitals, clinics, pet hospitals
or projects with small test requirements.
For the point-of-care testing chemiluminescence detector, its light signal collector
accounts for a large proportion of the total device cost, so only one light signal collector
is installed for this kind of device due to the consideration of device cost. Then, how to
use a light signal collector to measure multiple samples at the same time to obtain the
inspection results has become a technical problem that the technicians in the field need to
consider and solve.
In view of this, it is an object of the present invention to provide a measuring method and
device for a chemiluminescence detector, which realizes that the chemiluminescence
detector can measure a plurality of samples to obtain inspection results by using one light
sensor, thereby saving device cost and improving product competitiveness.
To achieve the object above, the present invention provides the following technical
protocol:
A measuring method of a chemiluminescence detector comprises:
Controlling a light sensor to sequentially collect the luminescence of samples in each
measured channel according to preset rules and respectively outputting luminescence
signal values representing the luminescence intensity of samples in the measured
channels; The chemiluminescence detector comprises a plurality of measured channels,
and samples are respectively placed in each measured channel;
Using an algorithm matched with a preset rule to calculate the calculated value of the
luminous signal of each measured channel according to the luminous signal value data
corresponding to each measured channel;
According to the calculated value of luminous signal of each measured channel and the
relationship between the specified analyte concentration value and the calculated value of
luminous signal, the specified analyte concentration value of samples in each measured
channel is calculated.
Preferably, the preset rule is that the light sensor sequentially collects the luminescence of
samples in each measured channel according to a preset sequence; The light sensor comes
back to the foremost one after collecting the last measured channel, and sequentially collects the luminescence of samples in each measured channel again according to a preset sequence.
Preferably, according to the luminous signal value data corresponding to the measured
channel, the algorithm for calculating the luminous signal calculated value of the
measured channel is as follows:
X ,2 il
Where xj,1 represents the luminescence signal value when light sensor collects
luminescence for last time from the jth measured channel; Xj,2 represents the luminescence
signal value when the light sensor collects luminescence for next time from the jth
measured channel, and xj represents the calculated luminescence signal value of the jth
measured channel.
Preferably, the preset rules specifically include: when the light sensor collects
luminescence from a measured channel every time, it collects the luminescence from the
measured channel several times in turn and outputs several luminescence signal values
correspondingly;
According to the luminous signal value data corresponding to the measured channel, the
algorithm for calculating the luminous signal calculated value of the measured channel is
as follows:
x = xj,2,i - X ,l,i ) n;
Where xj,1,i represent luminescence signal value when the light sensor collected
luminescence from the jth measured channel for ith time in terms of last time, Xj,2,i
represent the luminescence signal value when the light sensor collected luminescence from the jth measured channel for ith time in terms of next time, n represents the light sensor collected luminescence for n times each time from jth measured channel, and xj represents the calculated value of luminescence signal of the jth measured channel.
Preferably, the time span from collecting luminescence of light sensor for last time from
jth measured channel for ith time to collecting luminescence for next time from jth
measured channel for ith time is equal to that of from collecting luminescence of light
sensor for last time from jth measured channel for (i+1)th time to colleting luminescence
for next time from jth measured channel for (i+l)th time; The jth measured channel
represents anyone of several measured channels, iSE [1, n)
. Preferably, the preset rule is that the light sensor sequentially collects the luminescence of
samples in each measured channel according to a preset order, and after the last measured
channel is collected, the luminescence of samples in each measured channel is
sequentially collected again according to the order opposite to the preset order starting
from the last measured channel.
According to the luminous signal value data corresponding to the measured channel, the
algorithm for calculating the luminous signal calculated value of the measured channel is
as follows:
=x,2 ±xj
Where xj,1 represents the luminescence signal value when light sensor collects
luminescence for last time from the jth measured channel; xj,2 represents the luminescence
signal value when the light sensor collects luminescence for next time from the jth
measured channel, and xj represents the calculated luminescence signal value of the jth
measured channel.
Preferably, the preset rules specifically include: when the light sensor collects
luminescence from a measured channel every time, it collects the luminescence from the
measured channel several times in turn and outputs several luminescence signal values
correspondingly;
According to the luminous signal value data corresponding to the measured channel, the
algorithm for calculating the luminous signal calculated value of the measured channel is
as follows:
x =( n X i=1 + i=1 x n~ I ;X
Where xj,1,i represent luminescence signal value when the light sensor collected
luminescence from the j th measured channel for ith time in terms of last time, Xj,2,i
represent the luminescence signal value when the light sensor collected luminescence
from the jth measured channel for ith time in terms of next time, n represents the light
sensor collected luminescence for n times each time from jth measured channel, and xj
represents the calculated value of luminescence signal of the jth measured channel.
Preferably, the light sensor sequentially collects the luminescence of samples in each
measured channel at equal time intervals.
A measuring device for chemiluminescence detector is used for implementing the
measuring method for chemiluminescence detector.
According to the technical protocol, the measuring method and device of the
chemiluminescence detector provided by the invention: firstly controlling a light sensor
to sequentially collect the luminescence of samples in each measured channel according
to preset rules, and respectively outputting luminescence signal values representing the
luminescence intensity of samples in the measured channels; Then using an algorithm matched with a preset rule to calculate the calculated value of the luminous signal of each measured channel according to the luminous signal value data corresponding to each measured channel; Furthermore, according to the calculated value of luminous signal of each measured channel and the relationship between the specified analyte concentration value and the calculated value of luminous signal, the specified analyte concentration value of samples in each measured channel is calculated.
Therefore, the measurement method and device of the chemiluminescence detector
realize that the chemiluminescence detector can measure a plurality of sample to obtain
inspection results by use one light sensor, thereby saving the cost of the device and
improving the competitiveness of products.
In order to explain the embodiments of the present invention or the technical protocol in
the prior art more clearly, the drawings required in the embodiments or the description of
the prior art will be briefly introduced below. Obviously, the drawings in the following
description are only some embodiments of the present invention, and other drawings can
be obtained according to these drawings for ordinary technicians in the field without
paying creative labor.
Brief description of the drawings Fig. 1 is a flow chart of a measurement method of a
chemiluminescence detector provided by an embodiment of the present invention.
In order to enable people in the technical field to better understand the technical protocol
in the present invention, the technical protocol in the embodiments of the present
invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative labor should belong to the scope of protection of the present invention.
Please refer to Fig. 1, which is a flow chart of a measurement method of a
chemiluminescence detector provided by an embodiment of the present invention. It can
be seen from the figure that the method includes the following steps:
S10: controlling a light sensor to sequentially collect the luminescence of samples in each
measured channel according to preset rules, and respectively outputting luminescence
signal values representing the luminescence intensity of samples in the measured
channels.
The chemiluminescence detector comprises a plurality of measured channels, and
samples to be measured can be placed in each measured channel. In practical application,
the number of measured channels included in the chemiluminescence detector can be
flexibly set according to the design requirements of the device, which is not specifically
limited in this embodiment. When applying measurement, the user can select any of
several measured channels, and place samples in each measured channel for
measurement. In one measurement, the samples placed in each channel can be the same
batch of samples or different batches of samples.
When the light sensor is turned on for measurement, the light sensor is controlled to
sequentially collect the luminescence of samples in each measured channel where
samples are placed according to preset rules, and the light sensor correspondingly outputs luminescence signal values representing luminescence intensity after each collection.
Optionally, the light sensor may be a photomultiplier tube installed in the
chemiluminescence detector.
Optionally, in a specific embodiment, the preset rule is that the light sensor sequentially
collects the luminescence of samples in each measured channel according to a preset
sequence, returns to the foremost measured channel after collecting the last measured
channel, and sequentially collects the luminescence of samples in each measured channel
again according to a preset sequence. Specifically, when the light sensor collects
luminescence from each measured channel in turn, no time interval can be set among the
collections of each measured channel, and the light sensor continuously and sequentially
collects the luminescence of samples in each channel. Or alternatively, when the light
sensor collects luminescence from each measured channel in turn, a time interval can be
set among the collections of each time, that is, there is a time interval between collecting
luminescence from the previous measured channel and collecting luminescence from the
next measured channel. Preferably, the light sensor sequentially collects the luminescence
of samples in each measured channel at equal time intervals.
Optionally, in another specific embodiment, the preset rule is that the light sensor
sequentially collects the luminescence of samples in each measured channel according to
a preset order, and after the last measured channel is collected, the luminescence of
samples in each measured channel is sequentially collected again according to the order
opposite to the preset order starting from the last measured channel. Specifically, when
the light sensor collects luminescence from each measured channel in turn, no time
interval can be set among the collections of each measured channel, and the light sensor continuously and sequentially collects the luminescence of samples in each channel. Or alternatively, when the light sensor collects luminescence from each measured channel in turn, a time interval can be set between each collection, that is, there is a time interval between collecting luminescence from the previous measured channel and collecting luminescence from the next measured channel.
S11: Using an algorithm matched with a preset rule to calculate the calculated value of
the luminous signal of each measured channel according to the luminous signal value
data corresponding to each measured channel;
In this step, an algorithm matching with the preset rules adopted when collecting samples
in each channel is used to calculate the calculated value of the luminous signal of each
measured channel according to the luminous signal value data collected corresponding to
the measured channel.
Specifically, corresponding to the preset rule applied in the first embodiment, the
algorithm for calculating the calculated value of the luminous signal of the measured
channel according to the luminous signal value data corresponding to the measured
channel is as follows:
X i ,2 , il
Where xj,1 represents the luminescence signal value when light sensor collects
luminescence for last time from the jth measured channel; Xj,2 represents the luminescence
signal value when the light sensor collects luminescence for next time from the jth
measured channel, and xj represents the calculated luminescence signal value of the jth
measured channel.
Further preferably, if the preset rules specifically include when the light sensor collects
luminescence from a measured channel every time, it collects the luminescence from the
measured channel several times in turn and outputs several luminescence signal values
correspondingly; Therefore, according to the luminous signal value data corresponding to
the measured channel, the algorithm for calculating the luminous signal calculated value
of the measured channel is as follows:
x = x,, - x~ )n;
Where xj,1,i represent luminescence signal value when the light sensor collected
luminescence from the j th measured channel for ith time in terms of last time, Xj,2,i
represent the luminescence signal value when the light sensor collected luminescence
from the jth measured channel for ith time in terms of next time, n represents the light
sensor collected luminescence for n times each time from jth measured channel, and xj
represents the calculated value of luminescence signal of the jth measured channel.
In practical application, the collection times, that is, the number of collected
luminescence signal values, can be flexibly set when luminescence for each measured
channel is collected, which is not specifically limited in this embodiment. Several
luminous signal values are collected in turn for each measured channel. By summing and
averaging several luminous signal values, it is helpful to reduce the measurement error
and improve the measurement accuracy and precision. Preferably, the time span from
collecting luminescence of light sensor for last time from jth measured channel for ithtime
to collecting luminescence for next time from jth measured channel for ith time is equal to
that of from collecting luminescence of light sensor for last time from jth measured
channel for (i+l)th time to collecting luminescence for next time from jth measured channel for (i+1)th time; The jth measured channel represents any one of several measured channels, iE [1, n). This is helpful to improve the accuracy of measurement.
In concrete implementation, when the light sensor collects each measured channel, it can
collect several times of luminescence in turn at equal time intervals, and acquire several
luminescence signal values.
Corresponding to the preset rule applied in the second embodiment, the algorithm for
calculating the calculated value of the luminous signal of the measured channel according
to the luminous signal value data corresponding to the measured channel is as follows:
X i ,2 , il
Where xj,1 represents the luminescence signal value when light sensor collects
luminescence for last time from the jth measured channel; Xj,2 represents the luminescence
signal value when the light sensor collects luminescence for next time from the jth
measured channel, and xj represents the calculated luminescence signal value of the jth
measured channel.
Further preferably, the preset rules specifically include: when the light sensor collects
luminescence from a measured channel every time, it collects the luminescence from the
measured channel several times in turn and outputs several luminescence signal values
correspondingly; Correspondingly, according to the luminous signal value data
corresponding to the measured channel, the algorithm for calculating the luminous signal
calculated value of the measured channel is as follows:
xi = ,2,i n
Where xj,1,i represent luminescence signal value when the light sensor collected
luminescence from the jth measured channel for ith time in terms of last time, Xj,2,i
represent the luminescence signal value when the light sensor collected luminescence
from the jth measured channel for ith time in terms of next time, n represents the light
sensor collected luminescence for n times each time from jth measured channel, and xj
represents the calculated value of luminescence signal of the jth measured channel.
In practical application, the collection times, that is, the number of collected
luminescence signal values, can be flexibly set when luminescence for each measured
channel is collected, which is not specifically limited in this embodiment. Several
luminous signal values are collected in turn for each measured channel. By summing and
averaging several luminous signal values, it is helpful to reduce the measurement error
and improve the measurement accuracy and precision. Preferably, in concrete
implementation, when the light sensor collects each measured channel, it can collect
several times of luminescence in turn at equal time intervals.
S12: According to the calculated value of luminous signal of each measured channel and
the relationship between the specified analyte concentration value and the calculated
value of luminous signal, the specified analyte concentration value of samples in each
measured channel is calculated.
In practical application, according to the sample to be tested and the analyte to be tested,
the relationship between the specified analyte concentration value corresponding to the
measured channel and the calculated value of luminescence signal can be selected and
set. The relationship between the specified analyte concentration value corresponding to the measured channel and the calculated value of the luminescence signal can be expressed as:
Yj=f (Xj) ;
xj represents the calculated value of the luminescence signal of the jth measured channel,
and Yj represents the specified analyte concentration value of the sample in the jth
measured channel.
According to the calculated luminous signal value of the corresponding measured channel
and the relationship between the specified analyte concentration value corresponding to
the channel and the calculated luminous signal value, the specified analyte concentration
of the sample in the channel is calculated.
It can be seen from the above, the measurement method of the chemiluminescence
detector provided in this embodiment realizes that the chemiluminescence detector can
measure a plurality of samples with one light sensor to obtain inspection results, which
can save the cost of the device and enhance the competitiveness of products.
The measurement method of this chemiluminescence detector will be described in detail
with two specific examples.
Example 1
After the reaction between the C-peptide detection reagent and the sample is completed
according to the specified process, C-peptide detection reagent and the sample is divived
into five parts which are put into five measuring channels of chemiluminescence detector
respectively. After starting the measurement, collecting 10 data in the first channel, which
are denoted as (x1, x1O), then move to the second channel to collect 10 data, which are
denoted as (x11, x20), and continue to collect 10 data in the third channel, the fourth channel and the fifth channel in turn, which are denoted as (x21, x50). This is the first time to collect data. Then return to the first channel, wait for 60s, and then repeatedly collect the data of the first to fifth channels in turn, which are recorded as (x51, x100), and this is the second time to collect data. After stopping collecting data, According to the algorithm of the first embodiment, each channel data is separately calibrated and corrected, and the calculated value of the luminous signal of each channel is calculated.
The calibration data of each channel is shown in Table 1-1 below; The test low values of
each channel are shown in Table 1-2 below (INS concentration is 15.8[IU/mL), and the
test high values of each channel are shown in Table 1-3 below (INS concentration is
253 U/mL).
Table 1-1
INS concentration( The first The second The third The fourth The fifth pU/mL) channel channel channel channel channel
1 2315 2221 2186 2073 2069 50 147598 140613 136688 130083 126144 100 286552 272145 264018 253941 244591 200 558532 532127 516594 494584 487156 300 837209 798112 779365 732573 715115
Table 1-2
Signal Output Deviation from Deviation from contrast INS (pU/mL) value concentration channel value the first channel 48242.66 15.62151 0.00% -1.13% thesecond 47318.42 16.24155 3.97% 2.79% channel the third channel 45559.2 16.32146 4.48% 3.30% the fourth 43372.93 15.46154 -1.02% -2.14% channel the fifth channel 37831.13 15.67495 0.34%o -0.79%o
CV 2.46%
Table 1-3
Signal Output Deviation from Deviation from contrast INS (gU/mL) value concentration channel value The first channel 696832.4 249.1689 0.00% -1.51% The second 681852.6 255.8985 2.70% 1.15% channel The third channel 678594.6 261.3468 4.89% 3.30%
616503.6 250.9264 0.71% -0.82% Tcha nulh The fifth channel 612588.9 255.0165 2.35% 0.80% CV 1.87%
It can be seen from the table data above that the deviation between the concentration
value output by the measurement method of this embodiment and the reference sample
value is within the controllable range, and the deviation between each channel is within
the controllable range.
Example 2
After the reaction between the C-peptide detection reagent and the sample is completed
according to the specified process, C-peptide detection reagent and the sample is divided
into six parts which are put into six measuring channels of chemiluminescence detector
respectively. After starting the measurement, collecting 10 data in the first channel, which
are denoted as (x1, x1O), then move to the second channel to collect 10 data, which are
denoted as (x11, x20), and continue to collect 10 data in the third channel, the fourth
channel and the fifth channel in turn, which are denoted as (x21, x50). This is the first
time to collect data. Without returning to the first channel, immediately collect 10 data in
the sixth channel, which are recorded as (x61, x70), and then move to the fifth channel to
collect 10 data, which are recorded as (x71, x80), and continue to collect the data of the fourth, third, second and first channels (x81, x120) in turn. This time is the second time to collect data and stop collecting data.
According to the algorithm of the second embodiment above, the data of each channel is
calibrated and corrected separately, and the calculated value of the luminous signal of
each channel is calculated. The calibration data of each channel is shown in Table 2-1
below; The test low values of each channel are shown in Table 2-2 below (CP
concentration is 0.49 ng/mL), and the test high values of each channel are shown in Table
2-3 below (CP concentration is 12.6 ng/mL).
Table 2-1
CP concentration The first The second The third The fourth The fifth The sixth (ng/mL) channel channel channel channel channel channel 0.1 945 943 962 956 976 987 5.4 219707 221001 224201 226623 230114 233009 10.5 478975 483338 497893 503261 510064 517185 15.6 711332 718976 738441 746232 758119 767851 20.7 921853 931756 957612 968775 983835 995515 Table 2-2
Signal Output Deviation from Deviation from contrast CP (ng/mL) value concentration channel value The first channel 13740.32 0.485 0.00% -1.09% The second 14595.18 0.513 5.78% 4.63% channel The third channel 13796.06 0.507 4.57% 3.43% The fourth 13041.26 0.491 1.38% 0.27% channel The fifth channel 12652.61 0.483 -0.25% -1.33% Thesixth 3966.28 0.501 3.47% 2.34% channel CV 2.43%o
Table 2-3
CP (ng/mL) Signal Output Deviation from Deviation from contrast value concentration channel value The first channel 542661.4 12.14 0.00% -3.68% The second 572874.7 12.67 4.44% 0.59% channel The third channel 582421.7 12.55 3.39% -0.41% The fourth 578364 12.33 1.57% -2.16% channel The fifth channel 590004 12.38 2.05% -1.71% Thesixth 05461.3 12.55 3.40% -0.41% channelI CV 1.55% It can be seen from the table data above that the deviation between the concentration
value output by the measurement method of this embodiment and the reference sample
value is within the controllable range, and the deviation between each channel is within
the controllable range.
Accordingly, the embodiment of the present invention also provides a
chemiluminescence detector measuring device, which is used for executing the above
mentioned chemiluminescence detector measuring method.
The measuring method and device of the chemiluminescence detector provided by the
invention: firstly controlling a light sensor to sequentially collect the luminescence of
samples in each measured channel according to preset rules, and respectively outputting
luminescence signal values representing the luminescence intensity of samples in the
measured channels; Then using an algorithm matched with a preset rule to calculate the
calculated value of the luminous signal of each measured channel according to the
luminous signal value data corresponding to each measured channel; Furthermore,
according to the calculated value of luminous signal of each measured channel and the
relationship between the specified analyte concentration value and the calculated value of luminous signal, the specified analyte concentration value of samples in each measured channel is calculated.
The measurement device of the chemiluminescence detecotor of the embodiment realizes
that the chemiluminescence detector can measure a plurality of samples to obtain
inspection results by using one light sensor, thereby saving the cost of the device and
improving the competitiveness of products.
The measurement method and device of the chemiluminescence detector provided by the
present invention are described in detail above. In this paper, a specific example is
applied to illustrate the principle and implementation of the present invention, and the
explanation of the embodiments above is only used to help understand the method and
core idea of the present invention. It should be pointed out that, for those of ordinary skill
in the technical field, without departing from the principle of the present invention,
several improvements and modifications can be made to the present invention, and these
improvements and modifications also fall within the protection scope of the claims of the
present invention.
Claims (10)
1. A measuring method of a chemiluminescence detector is characterised by following
steps:
controlling a light sensor to sequentially collect the luminescence of samples in each
measured channel according to preset rules, and respectively outputting luminescence
signal values representing the luminescence intensity of samples in the measured
channels; The chemiluminescence detector comprises a plurality of measured channels,
and samples are respectively placed in each measured channel;
Using an algorithm matched with a preset rule to calculate the calculated value of the
luminous signal of each measured channel according to the luminous signal value data
corresponding to each measured channel;
According to the calculated value of luminous signal of each measured channel and the
relationship between the specified analyte concentration value and the calculated value of
luminous signal, the specified analyte concentration value of samples in each measured
channel is calculated.
2. The measuring method of a chemiluminescence detector according to claim 1,
characterized in that the preset rule is that the light sensor sequentially collects the
luminescence of samples in each measured channel according to a preset sequence; The
light sensor comes back to the foremost one after collecting the last measured channel,
and sequentially collects the luminescence of samples in each measured channel again
according to a preset sequence.
3. The measuring method of a chemiluminescence detector according to claim 2, wherein
according to the luminous signal value data corresponding to the measured channel, the algorithm for calculating the luminous signal calculated value of the measured channel is as follows:
X i ,2 il
Where xj,1 represents the luminescence signal value when light sensor collects
luminescence for last time from the jth measured channel; Xj,2 represents the luminescence
signal value when the light sensor collects luminescence for next time from the jth
measured channel, and xj represents the calculated luminescence signal value of the jth
measured channel.
4. The measuring method of chemiluminescence detector according to claim 2, wherein
the preset rules specifically include: when the light sensor collects luminescence from a
measured channel every time, it collects the luminescence from the measured channel
several times in turn and outputs several luminescence signal values correspondingly;
According to the luminous signal value data corresponding to the measured channel, the
algorithm for calculating the luminous signal calculated value of the measured channel is
as follows:
x= x,2,i - X ,,i )n;
Where xj,1,i represent luminescence signal value when the light sensor collected
luminescence from the jth measured channel for the ith time in terms of last time, Xj,2,i
represent the luminescence signal value when the light sensor collected luminescence
from the jth measured channel for the ith time in terms of next time, n represents the light
sensor collected luminescence for n times each time from jth measured channel, and xj
represents the calculated value of luminescence signal of the jth measured channel.
5. The measuring method of chemiluminescence detector according to claim 4,
characterized in that the time span from collecting luminescence of light sensor for last
time from jth measured channel for ith time to collecting luminescence for next time from
jthmeasured channel for ithtime is equal to that of from collecting luminescence of light
sensor for last time from jth measured channel for (i+1)th time to colleting luminescence
for next time from jth measured channel for (i+l)th time; The jth measured channel
represents anyone of several measured channels, iE [1, n)
.
6.The measurement method of chemiluminescence detector according to claim 1,
characterized in that the preset rule is that the light sensor sequentially collects the
luminescence of samples in each measured channel according to a preset order, and after
the last measured channel is collected, the luminescence of samples in each measured
channel is sequentially collected again according to the order opposite to the preset order
starting from the last measured channel.
7. The measuring method of a chemiluminescence detector according to claim 6, wherein
according to the luminous signal value data corresponding to the measured channel, the
algorithm for calculating the luminous signal calculated value of the measured channel is
as follows:
Xi,2 + 11
Where xj,1 represents the luminescence signal value when light sensor collects
luminescence for last time from the jth measured channel; Xj,2 represents the luminescence
signal value when the light sensor collects luminescence for next time from the jth
measured channel, and xj represents the calculated luminescence signal value of the jth
measured channel.
8. The measuring method of chemiluminescence detector according to claim 6, wherein
the preset rules specifically include: when the light sensor collects luminescence from a
measured channel every time, it collects the luminescence from the measured channel
several times in turn and outputs several luminescence signal values correspondingly;
According to the luminous signal value data corresponding to the measured channel, the
algorithm for calculating the luminous signal calculated value of the measured channel is
as follows:
x = nx + nxI n;
Where Xj,1,i represent luminescence signal value when the light sensor collected
luminescence from the jth measured channel for the ith time in terms of last time; Xj,2,i
represent the luminescence signal value when the light sensor collected luminescence
from the jth measured channel for the ith time in terms of last time next time, n represents
the light sensor collected luminescence for n times each time from jth measured channel,
and Xj represents the calculated value of luminescence signal of the jth measured channel.
9. The measurement method of chemiluminescence detector according to any one of
claims 1-8, characterized in that the light sensor sequentially collects the luminescence of
samples in each measured channel at equal time intervals.
10. A measuring device for chemiluminescence detector, which is used for implementing
the measuring method for chemiluminescence detector according to any one of claims 1
9.
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