AU2021100464A4 - A preparation method of an anti-freezing and anti-drying agent for grapevines - Google Patents

A preparation method of an anti-freezing and anti-drying agent for grapevines Download PDF

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AU2021100464A4
AU2021100464A4 AU2021100464A AU2021100464A AU2021100464A4 AU 2021100464 A4 AU2021100464 A4 AU 2021100464A4 AU 2021100464 A AU2021100464 A AU 2021100464A AU 2021100464 A AU2021100464 A AU 2021100464A AU 2021100464 A4 AU2021100464 A4 AU 2021100464A4
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Prior art keywords
grapevines
freezing
drying agent
parts
preparation
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AU2021100464A
Inventor
Shuzhen Jiao
Lan Wang
Weirong XU
Wenkong Yao
Junxiang ZHANG
Ningbo Zhang
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Yinchuan Industrial Technology Research Institute
Ningxia University
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Yinchuan Industrial Tech Research Institute
Ningxia University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/02Cultivation of hops or vines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/24Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N2300/00Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a preparation method of an anti-freezing and anti-drying agent for grapevines, which comprises the following raw materials: paraffin, glycerine, vegetable oil, sodium alginate, polysaccharide, abscisic acid, Terminalia chebula Retz. powder and microcrystalline cellulose. The preparation method comprises: uniformly mixing glycerine with water, and then adding sodium alginate, polysaccharide, abscisic acid, Terminalia chebula Retz. powder and microcrystalline cellulose in turn under the stirring condition, and uniformly stirring. Adding paraffin and vegetable oil under the condition of high-speed stirring, and uniformly mixing to obtain the anti-freezing and anti-drying agent for grapevines. The anti freezing and anti-drying agent of the invention is coated on the surface of the grapevines unearthed in spring, so that the grapevines can resist the cold spell in later spring, prevent the branches from losing water and drying out, and greatly reduce the labor cost.

Description

A preparation method of an anti-freezing and anti-drying agent for grapevines
TECHNICAL FIELD
The invention belongs to the field pf anti-freezing and anti-drying agent, in particular to a
preparation method of an anti-freezing and anti-drying agent for grapevines.
BACKGROUND
There are more than 8000 kinds of grapes in the world, which are widely used in wine
making, fresh-eating, juice making, canning and rootstock varieties. Grape branches are
easily affected by climate in winter and spring because of their loose epidermal tissue and
large stomata, especially the frequent occurrence of "cold spell in later spring", which
leads to the injury of grape flower buds and fruitlet or drying phenomenon of branches
and vines, which seriously affects the yield and benefit of grapes. Anti-freezing and anti
drying is a crucial problem in the cultivation and production of grapes in spring, and it is
also one of the reasons that restrict the improvement of grape planting techniques.
In order to prevent drying of grapevines mother branches and the harm of cold spell in
later spring after being buried in soil for cold protection, most grape growers adopt delay
ditching, and cover the grapes with soil and hay before the arrival of "cold spell in later
spring". Fumigation anti-freezing or irrigation strategies are adopted for unearthed
grapevines. It also sprays biostimulants such as amino oligosaccharide, fish protein,
alginic acid and amino acid before flowering to increase the toughness of cell membrane
and improve the cold resistance of grapevines. These methods have a certain effect on
preventing the harm of "cold spell in later spring", but the comprehensive effect is not
good. In addition, in early winter, before ditching and burying, the mother branches should be pruned reasonably, and the management should be strengthened to ensure the maturity of branches and vines. After burying, we should fill the freezing water thoroughly to protect against cold, etc., so as to ensure that the grapevines can be better prevented from drying out and cold spell in later spring after being unearthed in winter.
However, this overwintering method has some problems, such as labor-intensive and over
investment. Moreover, the method itself has defects. In the late autumn and early winter,
when grapes are buried under the trellis, if the grapevines are buried too early, the growth
of plants will be affected. If it is buried too late, once the earth cools and freezes, it will
be difficult to bury the grapes. When grapes are unearthed and put on trellis in spring, if
they are unearthed too early, the grapevines may suffer from drying out, or damage of
cold spell in later spring. If they are unearthed too late, the problem of bud rot may occur.
This method is greatly influenced by climate factors. Other treatment methods, such as
using electric heating cable to prevent freezing injury of grapevines, or using plastic
greenhouse to resist wind and cold and prevent water loss and frostbite of grapevines,
these methods can only be used on a small scale, and the cost is too high to be used in
large-scale grape plantations.
SUMMARY
In order to solve the above technical problems, the present invention provides a
preparation method of an anti-freezing and anti-drying agent for grapevines, which can
realize the purpose of preventing cold spell in later spring and anti-drying of grapevines
by smearing or spraying the anti-freezing and anti-drying agent on the surface of
grapevines after they are unearthed, thereby greatly reducing losses.
The invention relates to a preparation method of an anti-freezing and anti-drying agent
for grapevines, which comprises the following raw materials in parts by mass: paraffin 5
parts, glycerine 1-5 parts, vegetable oil 10-15 parts, sodium alginate 1-3 parts,
polysaccharide 5-10 parts, abscisic acid 1-2 parts, Terminalia chebula Retz. Powder 1-2
parts and microcrystalline cellulose 0.05-0.1 parts.
The preparation method comprises the following steps:
(1) Evenly mixing glycerine with water, and then adding sodium alginate,
polysaccharide, abscisic acid, Terminalia chebula Retz. powder and microcrystalline
cellulose in turn under the stirring condition, and evenly stirring.
(2) Adding paraffin and vegetable oil under the condition of high-speed stirring, and
uniformly mixing to obtain the anti-freezing and anti-drying agent for grapevines.
Furthermore, the mixing volume ratio of water and glycerine in the step (1) is (5-10): 1.
Furthermore, the stirring speed in the step (1) is 500-10OOr/min.
Furthermore, the high-speed stirring condition in the step (2) is 1500-2500r/min.
The invention also provides the anti-freezing and anti-drying agent for grapevines
prepared by the preparation method of the present invention.
The invention also provides the application of the anti-freezing and anti-drying agent for
grapevines in anti-freezing and anti-drying.
Furthermore, the anti-freezing and anti-drying agent for grapevines shall be sprayed on
the surface of the grapevines in clear and windless weather in spring.
Compared with the prior art, the invention has the following beneficial effects:
(1) The emulsion particles prepared by paraffin, glycerine and vegetable oil are fine and
uniform, and have good stability, which can form a protective film on the surface of the
vine, thus playing the role of sealing and keeping water, and slowing down the
phenomenon that the vine is drained due to excessive water loss caused by the influence
of cold and dry climate after the vine is unearthed.
(2) Sodium alginate is a kind of natural polysaccharide, which is stable in performance,
easy to dissolve and sticky. The addition of sodium alginate to the anti-freezing and anti
drying agent of the invention can increase the viscosity of the product, make its
components more easily adhere to the vine, and improve the film-forming property and
toughness of the anti-freezing and anti-drying agent, thus prolonging the residence time
of the anti-freezing and anti-drying agent on the vine.
(3) polysaccharide provides nutrients for plants during cold spring, ensures the normal
growth of plants, and protects the normal exchange of plant cells with external
substances.
(4) Abscisic acid, as a growth regulator, can prevent plants from stopping or delaying
their growth during cold spring.
(5) Terminalia chebula Retz. powder and microcrystalline cellulose can promote the
activation of biological enzymes in plants, prevent the formation of ice nucleation
bacteria, inhibit and destroy the ice-forming activity of frozen proteins, induce the
production of anti-freezing factors, increase the concentration of cell fluid, reduce the
freezing point, promote the formation of protective films on the surface of grapevines,
improve the water-holding capacity of grapevines and avoid heat loss.
DESCRIPTION OF THE INVENTION
Various exemplary embodiments of the present invention will be described in detail,
which should not be regarded as a limitation of the present invention, but rather as a more
detailed description of certain aspects, characteristics and embodiments of the present
invention.
It should be understood that the terms described in the present invention are only for
describing specific embodiments, and are not intended to limit the present invention. In
addition, as for the numerical range in the present invention, it should be understood that
every intermediate value between the upper limit and the lower limit of the range is also
specifically disclosed. Intermediate values within any stated value or stated range and
every smaller range between any other stated value or intermediate values within the
stated range are also included in the present invention. The upper and lower limits of
these smaller ranges can be independently included or excluded from the range.
Unless otherwise stated, all technical and scientific terms used herein have the same
meanings as commonly understood by those skilled in the art to which the present
invention relates. Although the present invention only describes preferred methods and
materials, any methods and materials similar or equivalent to those described herein may
be used in the practice or testing of the present invention. All documents mentioned in
this specification are incorporated by reference to disclose and describe methods and/or
materials related to the documents. In case of conflict with any incorporated documents,
the contents of this specification shall prevail.
Without departing from the scope or spirit of the invention, it is obvious to those skilled
in the art that many modifications and changes can be made to the specific embodiments
of the specification of the invention. Other embodiments derived from the description of
the present invention will be apparent to the skilled person. The specification and
embodiments of this application are only exemplary.
As used herein, "including", "having", "containing", etc. are all open terms, which means
including but not limited to.
Embodiment 1
The raw materials are weighed as follows: paraffinlO parts, glycerine 5 parts, vegetable
oil 10 parts, sodium alginate 3 parts, polysaccharide 10 parts, abscisic acid 2 parts,
Terminaliachebula Retz. powder 2 parts and microcrystalline cellulose 0.1 parts.
Uniformly mixing glycerine with 5 times of water, and adding sodium alginate,
polysaccharide, abscisic acid, Terminalia chebula Retz. powder and microcrystalline
cellulose in turn under the stirring condition of 500r/min, and stir uniformly.
Adding paraffin and vegetable oil under the condition of high-speed stirring at 1500r/min,
and uniformly mixing to obtain the anti-freezing and anti-drying agent for grapevines.
Embodiment 2
The raw materials are weighed as follows: paraffin 8 parts, glycerine 3 parts, vegetable
oil 15 parts, sodium alginate 1 part, polysaccharide 8 parts, abscisic acid 1 part,
Terminaliachebula Retz. Powder 1 part and microcrystalline cellulose 0.05 parts.
Uniformly mixing glycerine with 8 times of water, and then adding sodium alginate,
polysaccharide, abscisic acid, Terminalia chebula Retz. powder and microcrystalline
cellulose in turn under the stirring condition of 800r/min, and stir uniformly.
Adding paraffin and vegetable oil under the condition of high-speed stirring at 2000r/min,
and uniformly mixing to obtain the anti-freezing and anti-drying agent for grapevines.
Embodiment 3
The raw materials are weighed as follows: paraffin 5 parts, glycerine 1 part, vegetable oil
13 parts, sodium alginate 2 parts, polysaccharide 5 parts, abscisic acid 1.5 parts,
Terminaliachebula Retz. Powder 1.5 parts and microcrystalline cellulose 0.8 parts.
Uniformly mixing glycerine with 10 times of water, and then adding sodium alginate,
polysaccharide, abscisic acid, Terminalia chebula Retz. powder and microcrystalline
cellulose in turn under the stirring condition of 1000r/min, and stir uniformly.
Adding paraffin and vegetable oil under the condition of high-speed stirring at 2500r/min,
and uniformly mixing to obtain the anti-freezing and anti-drying agent for grapevines.
Embodiment 4
Same as embodiment 1, except that Terminalia chebula Retz. powder and
microcrystalline cellulose are omitted.
Embodiment 5
Same as embodiment 1, except that sodium alginate is omitted.
Embodiment 6
Same as embodiment 1, except that paraffin is omitted.
Embodiment 7
Same as embodiment 1, except that glycerine is omitted.
Embodiment 8
Same as embodiment 1, except that vegetable oil is omitted.
Embodiment 9
Same as embodiment 1, except that abscisic acid is omitted.
Embodiment 10
Same as embodiment 1, except that polysaccharide is omitted.
Embodiment of effect verification
A total of 165 wine grapevines in a 3-year-old vineyard in Yinchuan were divided into 11
groups, with 15 grapevines in each group on average. Before winter, the long shoots were
pruned, the upturned skin was removed, and they were buried for winter. After the
grapevines were unearthed in spring, the first to tenth groups were sprayed with the anti
freezing and anti-drying agent prepared in Embodiment 1-10, and the eleventh group was
used as a blank test without spraying after it was unearthed.
Yinchuan has a temperate continental climate with 38.5 degrees north latitude, 106.2
degrees east longitude and 1111 meters above sea level. It is coldest in January in winter,
with average temperature of -9°C and extreme low temperature below -22°C. After unearthed in spring, the temperature is 5-10°C, the humidity is 30-50%, during cold spell in later spring, the temperature is-3°C to -5°C, and the air humidity is 60-70%.
After cold spell in later spring, the survival rate of grapevines and the number of withered
plants were investigated. The results are shown in Table 1.
Table 1
Survival Rate Withered Plants
Embodiment 1 100% 0
Embodiment 2 100% 0
Embodiment 3 100% 0
Embodiment 4 75% 3
Embodiment 5 85% 5
Embodiment 6 78% 3
Embodiment 7 82% 4
Embodiment 8 79% 4
Embodiment 9 88% 3
Embodiment 10 89% 3
Embodiment 11 45% 8
The above contents are only preferred embodiments of the present invention, and is not
intended to limit the present invention. Any modifications, equivalent substitutions and
improvements made within the spirit and principles of the present invention shall be
included in the scope of protection of the present invention.

Claims (7)

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS
1. A preparation method of an anti-freezing and anti-drying agent for grapevines, which
is characterized by comprising the following raw materials in parts by mass: paraffin 5-10
parts, glycerine 1-5 parts, vegetable oil 10-15 parts, sodium alginate 1-3 parts,
polysaccharide 5-10 parts, abscisic acid 1-2 parts, Terminalia chebula Retz. Powder 1-2
parts and microcrystalline cellulose 0.05-0.1 parts.
The preparation method comprises the following steps:
(1) Evenly mixing glycerine with water, and then adding sodium alginate,
polysaccharide, abscisic acid, Terminalia chebula Retz. powder and microcrystalline
cellulose in turn under the stirring condition, and evenly stirring.
(2) Adding paraffin and vegetable oil under the condition of high-speed stirring, and
uniformly mixing to obtain the anti-freezing and anti-drying agent for grapevines.
2. The preparation method of the anti-freezing and anti-drying agent for grapevines
according to claim 1, characterized in that the mixing volume ratio of water and glycerine
instep (1) is (5-10): 1.
3. The preparation method of the anti-freezing and anti-drying agent for grapevines
according to claim 1, characterized in that the stirring speed in the step (1) is 500
1OOOr/min.
4. The preparation method of the anti-freezing and anti-drying agent for grapevines
according to claim 1, characterized in that the high-speed stirring condition in step (2) is
1500-2500r/min.
5. The anti-freezing and anti-drying agent for grapevines prepared by the preparation
method according to any one of claims 1-4.
6. The application of the anti-freezing and anti-drying agent for grapevines according to
claim 5 in preventing cold spell in later spring and anti-drying of grapevines.
7. The application of the anti-freezing and anti-drying agent for grapevines according to
claim 6, characterized in that the anti-freezing and anti-drying agent for grapevines shall
be sprayed on the surface of the grapevines in clear and windless weather in spring.
AU2021100464A 2021-01-25 2021-01-25 A preparation method of an anti-freezing and anti-drying agent for grapevines Ceased AU2021100464A4 (en)

Priority Applications (1)

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