AU2020370481B2 - Systems and devices for electroporation of cell-containing fluid - Google Patents

Systems and devices for electroporation of cell-containing fluid

Info

Publication number
AU2020370481B2
AU2020370481B2 AU2020370481A AU2020370481A AU2020370481B2 AU 2020370481 B2 AU2020370481 B2 AU 2020370481B2 AU 2020370481 A AU2020370481 A AU 2020370481A AU 2020370481 A AU2020370481 A AU 2020370481A AU 2020370481 B2 AU2020370481 B2 AU 2020370481B2
Authority
AU
Australia
Prior art keywords
electroporation
chamber
electrode
cartridge
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2020370481A
Other languages
English (en)
Other versions
AU2020370481A1 (en
Inventor
Nektaria ANDRONIKOU
Chee Wai Chan
Kok Shyong CHONG
Xavier De Mollerat Du Jeu
Ulrich Forke
Michael Gordon
Siu Wee Hon
Way Xuang LEE
Beng Heng Lim
Li Yang LIM
Yvonne PECK
Chris Rossman
Dhanaraj SHANMUGAM
Benyong SHI
Stacey TEO
Jun Yan Tham
Han Wei
Steven Huei YEO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Holdings Pty Ltd
Life Technologies Corp
Original Assignee
Life Technologies Holdings Pty Ltd
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Holdings Pty Ltd, Life Technologies Corp filed Critical Life Technologies Holdings Pty Ltd
Publication of AU2020370481A1 publication Critical patent/AU2020370481A1/en
Application granted granted Critical
Publication of AU2020370481B2 publication Critical patent/AU2020370481B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
AU2020370481A 2019-10-25 2020-10-23 Systems and devices for electroporation of cell-containing fluid Active AU2020370481B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962926078P 2019-10-25 2019-10-25
US62/926,078 2019-10-25
PCT/US2020/057138 WO2021081381A1 (en) 2019-10-25 2020-10-23 Systems and devices for electroporation of cell-containing fluid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2026203079 Division 2020-10-23

Publications (2)

Publication Number Publication Date
AU2020370481A1 AU2020370481A1 (en) 2022-05-26
AU2020370481B2 true AU2020370481B2 (en) 2026-04-02

Family

ID=73402180

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020370481A Active AU2020370481B2 (en) 2019-10-25 2020-10-23 Systems and devices for electroporation of cell-containing fluid

Country Status (10)

Country Link
US (2) US20210123009A1 (https=)
EP (1) EP4048776A1 (https=)
JP (2) JP7784374B2 (https=)
KR (1) KR20220088469A (https=)
CN (2) CN214361426U (https=)
AU (1) AU2020370481B2 (https=)
BR (1) BR112022007622A2 (https=)
CA (1) CA3154506A1 (https=)
DE (1) DE202020106067U1 (https=)
WO (1) WO2021081381A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12097345B2 (en) * 2018-06-01 2024-09-24 Penumbra, Inc. Infusion catheter and methods of use
EP4048776A1 (en) * 2019-10-25 2022-08-31 Life Technologies Corporation Systems and devices for electroporation of cell-containing fluid
USD943760S1 (en) * 2020-05-11 2022-02-15 Maxcyte, Inc. Electroporation processing assembly
USD944410S1 (en) 2020-05-11 2022-02-22 Maxcyte, Inc. Electroporation processing assembly
USD965170S1 (en) * 2020-10-23 2022-09-27 Life Technologies Corporation Electroporation device
US20230110090A1 (en) * 2021-10-08 2023-04-13 Life Technologies Holdings Pte Limited System and Method of Electroporation Protocol Optimization
TW202400835A (zh) 2022-03-09 2024-01-01 荷蘭商Ａｓｍ Ｉｐ私人控股有限公司 反應器系統、控制液體源的溫度之方法、及經調適以容納容器的裝置
WO2023230253A1 (en) * 2022-05-27 2023-11-30 Sqz Biotechnologies Company Cartridges and devices for use in a system for delivery of a payload into a cell
CN117431158A (zh) * 2022-07-14 2024-01-23 苏州壹达生物科技有限公司 一种流式电穿孔装置
US20260092248A1 (en) 2022-09-15 2026-04-02 Life Technologies Corporation Bioreactors operable in static and dynamic modes and methods of use
KR102609307B1 (ko) * 2022-10-24 2023-12-04 티큐브잇(주) 유전자 형질 감염 장치
CN115722292B (zh) * 2022-11-22 2023-12-29 山东省煤田地质局第一勘探队 一种地下水自然资源调查用分类收集装置及使用方法
CN120882852A (zh) * 2023-06-21 2025-10-31 隆萨科隆有限公司 多腔室电穿孔盒及其使用方法
WO2026043806A2 (en) 2024-08-19 2026-02-26 Life Technologies Corporation Systems, methods, and assemblies for cell processing
CN120028438B (zh) * 2025-04-23 2025-08-26 晋城市鑫环球铸造有限公司 一种基于球墨铸铁管生产的探伤检测设备及方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650305A (en) * 1996-05-15 1997-07-22 Health Research Inc. Method to produce hybrid cells by electrofusion
US6969604B1 (en) * 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US20170335269A1 (en) * 2014-12-02 2017-11-23 Jian Chen A Shock Tube and Cell Electroporation Device with the Shock Tube

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US6150148A (en) 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
US20050282283A1 (en) * 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
DK1766057T3 (en) * 2004-05-12 2015-03-02 Maxcyte Inc Methods and devices related to a regulated flowelektroporationskammer
WO2006112870A1 (en) * 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
WO2011050009A1 (en) * 2009-10-19 2011-04-28 Rational Biotechnology Inc. Method, device and apparatus for inducing self-adjusting cell electroporation
US9382510B2 (en) 2011-08-25 2016-07-05 Jian Chen Methods and devices for electroporation
US9204833B2 (en) * 2012-03-27 2015-12-08 Phlebotics, Inc. Cartridge for automated blood sampling system
US8766641B2 (en) * 2012-08-28 2014-07-01 Halliburton Energy Services, Inc. Determining surface wetting of rock with changing well fluids
CN104403943B (zh) * 2014-12-02 2016-06-15 陈剑 一种电击管和具有电击管的细胞电转仪
CA3017108A1 (en) * 2015-03-12 2016-09-15 The Trustees Of The University Of Pennsylvania System, method, and device for high-throughput, automated culturing of genetically modified organisms
JP6660057B2 (ja) 2015-12-11 2020-03-04 国立大学法人豊橋技術科学大学 エレクトロポレーション用電極及び外来物質導入装置
MX2019015505A (es) * 2017-06-30 2020-07-28 Inscripta Inc Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados.
CN109679844B (zh) 2017-10-19 2023-08-22 苏州壹达生物科技有限公司 一种流式电穿孔装置
JP2021506309A (ja) * 2017-12-20 2021-02-22 スクイーズ バイオテクノロジーズ カンパニー 細胞の中へのペイロードの送達のためのシステム
US20190225928A1 (en) * 2018-01-22 2019-07-25 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems comprising filtration devices
EP4048776A1 (en) * 2019-10-25 2022-08-31 Life Technologies Corporation Systems and devices for electroporation of cell-containing fluid

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650305A (en) * 1996-05-15 1997-07-22 Health Research Inc. Method to produce hybrid cells by electrofusion
US6969604B1 (en) * 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US20170335269A1 (en) * 2014-12-02 2017-11-23 Jian Chen A Shock Tube and Cell Electroporation Device with the Shock Tube

Also Published As

Publication number Publication date
AU2020370481A1 (en) 2022-05-26
WO2021081381A1 (en) 2021-04-29
JP2026040486A (ja) 2026-03-09
CA3154506A1 (en) 2021-04-29
US20210123009A1 (en) 2021-04-29
EP4048776A1 (en) 2022-08-31
CN214361426U (zh) 2021-10-08
KR20220088469A (ko) 2022-06-27
JP2023501142A (ja) 2023-01-18
DE202020106067U1 (de) 2020-12-04
JP7784374B2 (ja) 2025-12-11
BR112022007622A2 (pt) 2022-10-11
US20240263124A1 (en) 2024-08-08
CN112708552A (zh) 2021-04-27

Similar Documents

Publication Publication Date Title
AU2020370481B2 (en) Systems and devices for electroporation of cell-containing fluid
US12180453B2 (en) Systems, devices, and methods for electroporation within a cell processing system
JP7413408B2 (ja) 自動化された単一細胞処理のためのシステムおよび方法
JP2026062699A (ja) 細胞処理のためのシステム、デバイス、及び方法
US20240400964A1 (en) System and Method of Electroporation Protocol Optimization
EP3464548B1 (en) System for use in bioprocessing
JP6081070B2 (ja) 核酸検出カセット及び核酸検出装置
CA3205311A1 (en) Systems and methods for processing cells
US20200338563A1 (en) Method for controlling an analysis device and analysis system
EP3523033A1 (en) Cartridge for testing a sample and method for producing a cartridge of this kind
EP2923761B1 (en) Nucleic acid amplification reaction device and nucleic acid amplification method
US20230381770A1 (en) Sample Preparation Cartridge and System
US20260035648A1 (en) Cell culture containers and fluid handling for scalable cell manufacturing
US20250325753A1 (en) Syringe and syringe system for manipulating a fluid
EP3523032A2 (en) Cartridge for testing a sample
WO2025006533A1 (en) Assembly and system for controlled dispensing of fluid
ES2746180T3 (es) Sistema de procesamiento automatizado de muestras de plataforma abierta
Gransee et al. Automated DNA-preparation system for bacteria out of air sampler liquids