AU2016304599A1 - Antigen detection using photocleavable labels - Google Patents

Antigen detection using photocleavable labels Download PDF

Info

Publication number
AU2016304599A1
AU2016304599A1 AU2016304599A AU2016304599A AU2016304599A1 AU 2016304599 A1 AU2016304599 A1 AU 2016304599A1 AU 2016304599 A AU2016304599 A AU 2016304599A AU 2016304599 A AU2016304599 A AU 2016304599A AU 2016304599 A1 AU2016304599 A1 AU 2016304599A1
Authority
AU
Australia
Prior art keywords
ligand
antigen
sample
pct
photocleavable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2016304599A
Other languages
English (en)
Inventor
Xiyi CHEN
Stacie FRYE
Mimi Healy
Sophia PETRICHENKO
Jinchun Wang
Weidong Wu
Su Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agilent Technologies Inc
Original Assignee
Agilent Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agilent Technologies Inc filed Critical Agilent Technologies Inc
Publication of AU2016304599A1 publication Critical patent/AU2016304599A1/en
Assigned to AGILENT TECHNOLOGIES, INC. reassignment AGILENT TECHNOLOGIES, INC. Request for Assignment Assignors: LASERGEN, INC.
Priority to AU2022210222A priority Critical patent/AU2022210222B2/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/082—Active control of flow resistance, e.g. flow controllers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/302—Stain compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00138—Slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AU2016304599A 2015-08-06 2016-08-08 Antigen detection using photocleavable labels Abandoned AU2016304599A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2022210222A AU2022210222B2 (en) 2015-08-06 2022-08-01 Antigen detection using photocleavable labels

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562201978P 2015-08-06 2015-08-06
US62/201,978 2015-08-06
PCT/US2016/045981 WO2017024298A1 (en) 2015-08-06 2016-08-08 Antigen detection using photocleavable labels

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2022210222A Division AU2022210222B2 (en) 2015-08-06 2022-08-01 Antigen detection using photocleavable labels

Publications (1)

Publication Number Publication Date
AU2016304599A1 true AU2016304599A1 (en) 2018-02-15

Family

ID=56686978

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2016304599A Abandoned AU2016304599A1 (en) 2015-08-06 2016-08-08 Antigen detection using photocleavable labels
AU2022210222A Active AU2022210222B2 (en) 2015-08-06 2022-08-01 Antigen detection using photocleavable labels

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2022210222A Active AU2022210222B2 (en) 2015-08-06 2022-08-01 Antigen detection using photocleavable labels

Country Status (9)

Country Link
US (2) US10942176B2 (https=)
EP (1) EP3332253A1 (https=)
JP (1) JP6893914B2 (https=)
CN (1) CN107949790B (https=)
AU (2) AU2016304599A1 (https=)
BR (1) BR112018002525A2 (https=)
CA (2) CA3138794C (https=)
MX (1) MX2018001519A (https=)
WO (1) WO2017024298A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018200713A1 (en) * 2017-04-25 2018-11-01 Dana-Farber Cancer Institute, Inc. Compositions and methods for targeted tumor immunotherapy
EP3431173A1 (en) * 2017-07-19 2019-01-23 Bayer Pharma Aktiengesellschaft Continuous manufacture of guidance molecule drug conjugates
JP2021511799A (ja) * 2018-01-30 2021-05-13 ラッシュ ユニヴァーシティ メディカル センター 組織および細胞の多重分析のための連続染色
WO2019236522A1 (en) * 2018-06-04 2019-12-12 Dana-Farber Cancer Institute, Inc. Compositions and methods for targeted immunotherapy
CA3125920A1 (en) 2019-01-07 2020-07-16 Agilent Technologies, Inc. Compositions and methods for genomic dna and gene expression analysis in single cells
JP2020143015A (ja) * 2019-03-06 2020-09-10 ソニー株式会社 粒子標識用試薬、粒子染色方法、及び顕微鏡システム
EP3983803A2 (en) * 2019-06-14 2022-04-20 Akoya Biosciences, Inc. Multiplexed tissue imaging
CN111381034A (zh) * 2020-03-03 2020-07-07 武汉生之源生物科技股份有限公司 一种gpc-3抗体包被磁珠及其制备方法、试剂盒
US20230390773A1 (en) * 2020-10-05 2023-12-07 Clara Biotech, Inc. Apparatus, system, and method for analyte release
CN112595845B (zh) * 2020-12-09 2022-10-21 深圳普门科技股份有限公司 透明质酸检测试剂盒及检测系统
CN121248767A (zh) * 2025-08-25 2026-01-02 北京科技大学 用于免疫共沉淀测序的含光裂解基团抗体、方法及试剂盒

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US41115A (en) * 1864-01-05 Edward b
US6358689B1 (en) 1994-05-11 2002-03-19 Boston University Detection of markers in nascent proteins
US6589736B1 (en) 1994-11-22 2003-07-08 The Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
CN2444230Y (zh) * 2000-05-16 2001-08-22 陈智 免疫组织化学仪
US7154598B2 (en) 2002-07-12 2006-12-26 Decision Biomarkers, Inc. Excitation and imaging of fluorescent arrays
CN101203597A (zh) * 2005-04-21 2008-06-18 塞莱勒斯诊断公司 用于自动快速免疫组织化学的芯吸盒方法和设备
AU2006324488B2 (en) 2005-12-12 2013-03-21 Abcam Limited Production of conjugates
ES2567402T3 (es) 2006-05-30 2016-04-22 Genentech, Inc. Anticuerpos anti CD22, sus inmunoconjugados y usos de los mismos
US20090203023A1 (en) 2008-02-11 2009-08-13 University Of Wyoming Concentrating Microorganisms in Aqueous Solution Prior to Selective Staining and Detection
US20090253163A1 (en) 2008-04-02 2009-10-08 General Electric Company Iterative staining of biological samples
KR101677772B1 (ko) 2008-06-11 2016-11-18 레이저젠, 인코퍼레이티드 뉴클레오타이드 및 뉴클레오사이드 그리고 시퀀싱에서의 이들의 이용 방법
DE102008062372B3 (de) * 2008-12-17 2010-06-17 Medizinische Hochschule Hannover Nachweiskonjugat und Verfahren zur Analyse
KR101066247B1 (ko) * 2009-08-04 2011-09-20 서울대학교산학협력단 멀티 루프 코일을 구비하는 판재용 비접촉식 트랜스듀서
CN102230895B (zh) 2011-03-31 2014-10-29 中国科学院合肥物质科学研究院 一种利用荧光蛋白作为荧光探针测量电离辐射下细胞氧化胁迫损伤的方法
MX342195B (es) 2011-09-13 2016-09-20 Lasergen Inc Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.
KR101969847B1 (ko) * 2012-10-30 2019-04-17 삼성전자주식회사 동일 검체에서 여러 항원을 검출하기 위한 순차적 다중 면역염색법
EP3126512B1 (en) * 2014-02-26 2019-04-17 Ventana Medical Systems, Inc. Photo-selective method for biological sample analysis field

Also Published As

Publication number Publication date
AU2022210222B2 (en) 2025-09-18
EP3332253A1 (en) 2018-06-13
CA3138794A1 (en) 2017-02-09
CN107949790A (zh) 2018-04-20
WO2017024298A1 (en) 2017-02-09
CA2993993A1 (en) 2017-02-09
US20210181191A1 (en) 2021-06-17
CA3138794C (en) 2024-01-23
JP6893914B2 (ja) 2021-06-23
US20170052175A1 (en) 2017-02-23
US10942176B2 (en) 2021-03-09
BR112018002525A2 (pt) 2018-09-25
CN107949790B (zh) 2024-07-26
AU2022210222A1 (en) 2022-08-25
MX2018001519A (es) 2018-08-01
JP2018523826A (ja) 2018-08-23
CA2993993C (en) 2022-01-04

Similar Documents

Publication Publication Date Title
US20210181191A1 (en) Antigen detection using photocleavable labels
US20260035738A1 (en) Multiplexed imaging with enzyme mediated amplification
JP7849956B2 (ja) 横断組織切片のユーザー定義領域における複数のタンパク質の同時定量
ES3008686T3 (en) Multiplex capture of gene and protein expression from a biological sample
ES2975362T3 (es) Cuantificación simultánea de la expresión génica en una región definida por el usuario de un tejido seccionado transversalmente
US7660454B2 (en) Process for identifying FISH signals
US20230193363A1 (en) METHOD TO GENERATE BIOCOMPATIBLE DENDRITIC POLYMERS FOR ANALYTE DETECTION WITH MULTIMODAL LABELING Ahttps://dav.uspto.gov/prex/index.html#domesticContinuityND SIGNAL AMPLIFICATION
US20250092441A1 (en) Sequential staining for multiplex analyses of tissues and cells
CN106715724A (zh) 用于检测第一和第二表位的空间接近性的方法
JP2012518613A (ja) スイッチング可能な親和性結合剤
US20140134645A1 (en) Method of isolating or counting target cells by using photocleavable linker coupled with fluorescent dye
CN101400805A (zh) 使用共价结合荧光团合并免疫染色法和荧光原位杂交法的方法
US20140057282A1 (en) Switchable affinity binders
WO2022118862A1 (ja) 生体分子構造検出用プローブ、生体分子構造検出用キット、及び生体分子構造の検出方法
Abuknesha et al. AMC-anti-FITC conjugates: novel reagents for amplified immunochemical techniques. Immunofluorescent staining of human fibroblasts

Legal Events

Date Code Title Description
PC1 Assignment before grant (sect. 113)

Owner name: AGILENT TECHNOLOGIES, INC.

Free format text: FORMER APPLICANT(S): LASERGEN, INC.

MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted