AU2013202662B2 - Hemostatic compositions and therapeutic regimens - Google Patents

Abstract

A poly-p-1--4-N-acetylglucosamine composition comprising biocompatible poly- 1--4-N-acetylglucosamine fibers, wherein (i) the majority of the fibers are less than about 15 5 pm in length, and (ii) the composition (a) increases metabolic rate of serum-starved human umbilical cord vein endothelial cells in a MTT assay and/or does not rescue apoptosis of serum-starved human umbilical cord vein endothelial cells in a trypan blue exclusion test, and (b) is non-reactive when tested in an intramuscular implantation test. <filename>

Description

HEMOSTATIC COMPOSITIONS AND THERAPEUTIC REGIMENS This application is a divisional of Australian Patent No. 2008219065, the entire contents of which are to be taken as incorporated herein by this reference. 1. FIELD [0001] The application relates generally to the field of hemostasis, including methods, compositions, and devices that can be employed to achieve hemostasis at an increased rate or in a reduced period of time. More specifically, the application describes hemostatic compositions that can be applied to wounds and therapeutic regimens for wound treatment, The hemostatic compositions can comprise one or more biocompatible polymers or fibers, such as poly p-1--4 N-acetylglucosamine dressings. Optionally, the poly p-1--4-N-acetylglucosamine dressings comprise irradiated poly P--1--4-N-acetylglucosamine of reduced molecular weight and length. 2. BACKGROUND [0002] Wound healing, or wound repair, is the body's natural process of regenerating dermal and epidermal tissue. After a wound occurs, a set of complex biochemical events takes place in a closely orchestrated cascade to repair the damage. These events overlap in time and may be artificially categorized into separate steps: the inflammatory, proliferative, and maturation and remodeling phases. In the inflammatory phase, bacteria and debris are phagocytized and removed, and factors are released that cause the migration and division of cells involved in the proliferative phase. The proliferative phase is characterized by angiogenesis (formation of new blood vessels from endothelial cells), fibroplasia, collagen deposition, granulation tissue formation, epithelialization, and wound contraction. In the maturation and remodeling phase, collagen is remodeled and realigned along tension lines and cells that are no longer needed are removed by apoptosis. [0003] There are two types of wounds, open and closed. Open wounds are classified according to the object that caused the wound. For example, incisions or incised wounds (including surgical wounds) are caused by a clean, sharp-edged object such as a knife, a razor or a glass splinter. Lacerations are irregular wounds caused by a blunt impact to soft tissue which lies over hard tissue (e.g., laceration of the skin covering the skull) or tearing of skin and other tissues such as caused by childbirth. Abrasions or grazes are superficial wounds in which the topmost layer of the skin (the epidermis) is scraped off. Puncture wounds are caused by an object puncturing the skin, such as a nail or needle. Penetration wounds are 1 WO 2008/103345 PCT/US2008/002172 caused by an object such as a knife entering the body. Gunshot wounds are caused by a bullet or similar projectile driving into (e.g., entry wound) and/or through the body (e.g., exit wound). In a medical context, all stab wounds and gunshot wounds are considered major wounds. Open wounds also include burn wounds induced by thermal, chemical, or electrical injury. [00041 Closed wounds include contusions (more commonly known as a bruise, caused by blunt force trauma that damages tissue under the skin), hematoma (also called a blood tumor, caused by damage to a blood vessel that in turn causes blood to collect under the skin), and crushing injuries (caused by a great or extreme amount of force applied over a long period of time). [00051 Chronic wounds are wounds that have failed to proceed through an orderly and timely series of events to produce a durable structural, functional, and cosmetic closure. Many chronic wounds are cutaneous wounds or ulcers, caused by factors such as diabetes, venous stasis, arterial insufficiency, or pressure. Certain cutaneous wounds are burn wounds, induced by thermal, chemical, or electrical injury. Chronic wounds are the source of significant pain and suffering. If left without treatment they can cause life threatening complications, reduce the rate of recovery or worsen other health conditions. Intensive and effective treatment can help restore skin integrity, and avoid unwanted health problems. While these wounds are inflicted by different causes, the wound healing process and the wound treatment strategies are similar in many respects. [0006] Bed sore are one type of pressure ulcers which may significantly reduce the quality of life and negatively affect general prognosis. Bed sores are localized areas of skin injury that develop when soft tissue is compressed between a bony prominence and an hard surface for a long time. Bed sores usually develop from laying or sitting for a prolonged period without changing the body posture. For those who are bed-bound, pressure sores are most likely to form on or around the heels, the hip-bone, and the lower back or tailbone. Pressure ulcers may also develop in a variety of other areas, including the spine, ankles, knees shoulders, and head, depending upon the position of the patient. If left without treatment, bedsores may degenerate to the stage of decay of epithelial tissue, with inflammation, bacterial infection and other serious complications. The body's response to the infection often results in fever, shaking chills, changes in mental status, rapid pulse, and respiratory rate. 2 [00071 Temporary dressings, including interactive lNporry dressings, e intnAded to provide supportvecare until definitive closure cantbe accomplished. Temporary dressings ar.eexpected to function usabarier, much like human skin. AvSilable wound dressing are efctveto adegre but have significant shortcomings, such a high frequency ofdressing changes, wound drying or dressing adherence, hightrealmen costs, development ofa foreign body reation~ snd a low ra- of imrovemeni especially ins elderly patients. The foreign body recon*U begim as weud heading, Including accumulation of exudate at the lite of injury, infltraion ofinammatory cells to debride the area, and the formafion of granulation tissue. Howev, thepcrsistent prseneofa foreignbody cninhibit full hcalin Rlher than the reargpagnand reconsuctie that occurs in wound healing, the foreign body action is characterized bythe formation of foreign body giant cells, ea on of the foreign object, and chronic inflammatio. Encapsultion refer to the finn, generally avascular collegen shell deposited around a foreign body, ef-ectively isolating it fgom the host tisewis, This reponsewts developed as a ptotectiveaeasme. The foreign body tanction ear lead to chronic pain. [008] Hoaling time offa chronic noeed can ringe front a fewweeks to ayea, defending enthiesizeandtype of wound, Woundtfreatment'mnvolves -ay direct and indirect costs. Acording to the Ilvrnational Cammillce on Wound Managament (ICWM). omud dressingscompriseonly 10pecentto 15 percentolthetotal directtreatrncntcost (International Committee on Wound Mmnagement, 1994, Wounds 6(3):94-100). Inoontrat, a significantprcnage oftotal otisattribitedto careprov Mid salary andstaffexpenses (Intentional Committee on Wonid Monago ent, 1994. Wounds 60):94-100). [00091 There is aneed in the art for wound dressings tat do not induceforeig body reactions ordo so aaowrratethntradillonol wouid dressings. Suchwounddtsshig would need tao sigdonaless frequentbasisandreiduce healingtimeltoelonnre, and - luumarnateInlo mor eflbetive therayforthepatient and areduced cost ofcat. {0009A] The discussion ofdocuments, acts. materials. devices, articles and the like is included in this specification solely for the purse ofproviding a context for die present invention Ih is not suggested or represented that any or all ofthese matters formed part ofthe prior art baseor 6 were common general knowledge in the field relevant to lie present invention as it existed before he priority date ofeach claim ofthis app [cation [O009B1 Where the terms "comprise,'. "comprises", "comprised' or "comprising" are used in this specification (including the claims) they am to be interpreted as specifying the presence of lie stated features, inegers, steps or components, but not precluding the presence of one or more 10 other features, integers, steps or components, or group thereof. 3 3 SUMMARY [0010] The application is based, in part, on the inventors' discovery of the improved wound healing and reduced need for wound dressing changes when poly-p-1-4-N acetylghcosamine CpGlcNAc" or "NAG") is administered to wounds in a diabetic mouse model. These studies indicate that pGlcNAc can be advantageously used as a wound dressing for wounds without the need for frequent wound dressing changes that are required 3a WO 2008/103345 PCT/US2008/002172 of other wound dressings that are currently in use. Other polymers or fibers with similar characteristics to pGlcNAc may also be used in accordance with the methods described herein. [0011] The improved wound healing characteristics of pGlcNAc together with the reduced need for pGlcNAc wound dressing changes are especially pronounced when the pGlcNAc is irradiated to reduce its molecular weight and length. The inventors have discovered that irradiation reduces the molecular weight and length of pGlcNAc without disturbing the microstructure of the fibers, such that the infrared spectrum of the irradiated pGlcNAc is substantially similar or equivalent to that of the non-irradiated pGlcNAc. [00121 This reduced molecular weight pGlcNAc, referred to herein as "sNAG" (for short N-Acetyl Glucosamine), shows improved activity in promoting wound healing and no detectable foreign body reaction in treated animals. Thus, this polymer or fiber is particularly useful for treatment of wounds at a greater efficacy and reduced cost. [0013] Accordingly, described herein are methods for treating a wound in a patient, preferably a human subject, said method comprising topically applying a dressing to a wound in the patient, thereby treating the wound in the patient. Preferably, the application is repeated every 3 to 35 days (a significant improvement over the methods presently used in the clinic, in which the application is repeated every 2 days). The dressing materials are preferably polymers or fibers, as described in Section 5.2 below. Preferably, the polymers or fibers are irradiated to improve their efficacy, as described in Section 5.3 below. Preferably, the dressing is a dressing of biocompatible and/or immunoneutral poly-p-1-+4-N acetylglucosamine or a derivative thereof, as described in Section 5.2.1 below. Alternative frequencies of wound dressing changes or reapplications are described in Section 5.4 below. [0014] Described herein is a poly-p-1-+4-N-acetylglucosamine composition comprising biocompatible poly-p-1-+4-N-acetylglucosamine fibers, wherein (i) the majority of the fibers are less than about 15 um in length, and (ii) the composition (a) increases metabolic rate of serum-starved human umbilical cord vein endothelial cells in a MTT assay and/or does not rescue apoptosis of serum-starved human umbilical cord vein endothelial cells in a trypan blue exclusion test, and (b) is non-reactive when tested in an intramuscular implantation test. In certain embodiments, the composition increases metabolic rate of serum-starved human umbilical cord vein endothelial cells in a MTT assay and does not rescue apoptosis of serum starved human umbilical cord vein endothelial cells in a trypan blue exclusion test. In one 4 WO 2008/103345 PCT/US2008/002172 embodiment, the majority of the fibers have a thickness or diameter of about 1-2 pm. In one embodiment, at least 50% of the fibers are about 4 pm in length. [0015] Also described herein is a method for treating a wound in a human subject, comprising: (a) topically applying a dressing to a wound in a human subject in need thereof, wherein the dressing comprises a poly-p-1--+4-N-acetylglucosamine composition comprising biocompatible poly-p-1-+4-N-acetylglucosamine fibers, thereby treating the wound in said human subject. In certain embodiments, the majority of the fibers are less than about 15 pm in length, and the composition (a) increases metabolic rate of serum-starved human umbilical cord vein endothelial cells in a MTT assay and/or does not rescue apoptosis of serum-starved human umbilical cord vein endothelial cells in a trypan blue exclusion test, and (b) is non reactive when tested in an intramuscular implantation test. In certain other embodiments, the method further comprises: (b) repeating said application every 5 to 35 days. In one embodiment, the wound is a chronic wound. In one embodiment, the chronic wound is a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, or a pressure ulcer. In one embodiment, the chronic wound is a venous stasis ulcer. In another embodiment, the wound is a surgical wound or a burn wound. [00161 In addition, described herein is a method for producing a poly-p-1-4-N acetylglucosamine composition, said method comprises irradiating biocompatible poly-p 1-4-N-acetylglucosamine fibers, such that (i) the majority of the irradiated fibers are less than about 15 pm in length, and (ii) the composition (a) increases metabolic rate of serum starved human umbilical cord vein endothelial cells in a MTT assay and/or does not rescue apoptosis of serum-starved human umbilical cord vein endothelial cells in a trypan blue exclusion test, and (b) is non-reactive when tested in an intramuscular implantation test, thereby producing the poly-p-1--+4-N-acetylglucosamine composition. In certain embodiments, the poly-p-1-+4-N-acetylglucosamine fibers are irradiated as dried fibers. In one embodiment, the poly-0-1-+4-N-acetylglucosamine fibers are irradiated by gamma irradiation at 500-2,000 kgy. In certain other embodiments, the poly-p-1-4-N acetylglucosamine fibers are irradiated as wet fibers. In one embodiment, the poly-p-1--+4 N-acetylglucosamine fibers are irradiated by gamma irradiation at 100-500 kgy. 100171 Furthermore, described herein is a method for treating a wound in a human subject, comprising: (a) topically applying a dressing to a wound in a human subject in need thereof, wherein the dressing comprises biocompatible poly-p-1 -+4-N-acetylglucosamine, and (b) repeating said application every 5 to 35 days, thereby treating the wound in said 5 human subject, wherein the human subject is a diabetic, a smoker, a hemophiliac, an HIV infected person, an obese person, a person undergoing radiation therapy, or a person with venous stasis ulcer. In certain embodiments, the human subject is a person with venous stasis ulcer. In one embodiment, the wound is a chronic wound. In certain embodiments, the 5 chronic wound is a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, or a pressure ulcer. In one embodiment, the chronic wound is a venous stasis ulcer. In other embodiments, the wound is a surgical wound or a burn wound. [0018] Furthermore, described herein is a method for treating a chronic wound in a human subject, comprising: (a) topically applying a dressing to a chronic wound in a human subject in 10 need thereof, wherein the dressing comprises biocompatible poly--1--4-N acetylglucosamine, and (b) repeating said application every 5 to 35 days, thereby treating the chronic wound in said human subject, wherein the chronic wound is a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, or a pressure ulcer. In certain embodiments, the chronic wound is a venous stasis ulcer. 15 [0019] In certain embodiments, the dressing of step (a) is removed prior to step (b). In certain other embodiments, the dressing of step (b) is not removed prior to step (b). [0020] In certain embodiments, the poly- 0-1--4-N-acetylglucosamine is a microalgal poly-- 1--4-N-acetylglucosamine. In certain embodiments, the poly-- 1--4-N acetylglucosamine is not a crustacean poly-- 1--4-N-acetylglucosamine. 20 [0021] In certain embodiments, the poly- 0-1--4-N-acetylglucosamine comprises fibers, and wherein the poly--1--4-N-acetylglucosamine have been irradiated to reduce the length of the fibers. [0022] In certain embodiments, at least 75% of the dressing consists of poly--1--4-N acetylglucosamine. 25 [0023] In certain embodiments, the poly- 0-1--4-N-acetylglucosamine comprises deacetylated poly-0- 1--4-N-acetylgluco samine. In one embodiment, the deacetylated poly- 1--4-N-acetylglucosamine is 20-70% deacetylated. [0023a] Furthermore, described herein is a method for treating a chronic wound in a human subject, comprising: (a) topically applying a composition comprising biocompatible poly-3 30 1--4-N-acetylglucosamine fibers to a chronic wound in a human subject in need thereof, wherein the poly-3-1-4-N-acetylglucosamine comprises at least 70% of N-acetylglucosamine 6 monosaccharides, and (b) repeating said application every 5 to 18 days, wherein the application is repeated at least twice. (see spec at [00110] and [0084].) [0023b] Furthermore, described herein is a use of a composition comprising biocompatible poly-3-1--4-N-acetylglucosamine fibers, wherein the poly- -1-4-N-acetylglucosamine 5 comprises at least 70% of N-acetylglucosamine monosaccharides, in the manufacture of a medicament for treating a chronic wound in a human subject comprising: (a) topically administering the composition to a chronic wound in a human subject in need thereof, and (b) repeating said application every 5 to 18 days, wherein the application is repeated at least twice. 10 4. BRIEF DESCRIPTION OF THE FIGURES [0024] Fig. 1. Wound closure analysis of pGlcNAc treated and non-treated wounds. Standardized photographs were taken on the day of wounding (day 0) and twice a week 6a WO 2008/103345 PCT/US2008/002172 during the follow-up. Wound contraction (C), reepithelialization (E), and open wound (0) were studied as a percentage of the original wound area. [0025] Fig. 2. pGlcNAc induced faster wound closure. (A) The application of the pGlcNAc patch for 1 hour induced faster reduction of raw surface. (B) The 1 h group reached 90% wound closure faster than the NT group. (C) Reepithelialization was enhanced after 1 h patch application. (D) The lh group showed enhanced reepithelialization on day 14. (E) The application of the patch for 24h decreased wound contraction when compared to non-treated wounds. Mean values and standard deviations are shown. *p<0.01. [0026] Fig. 3. The Wound Watch staging system. (A) Ki-67 staining: the lh group showed enhanced cell proliferation on day 10 compared to the other groups. (B) PECAM-1 staining: the lh group showed enhanced neo-vascularization on day 10 compared to the other groups. (C) The plot combines the quantification of the two immunohistochemical markers, visually showing the difference between groups on study. The lh group dramatically differed from both the 24h and NT groups. Mean values and standard deviations are shown. *p<0.01. [00271 Fig. 4. Effect of irradiation on chemical and physical structure of pGlcNAc fibers. (A) Correlation between molecular weight of pGlcNAc and irradiation level. (B) Infrared (IR) spectrum of non-irradiated pGlcNAc slurry (top line), pGlcNAc slurry irradiated at 100 kGy (bottom line), and pGlcNAc slurry irradiated at 200 kGy (middle line). (C) Scanning electron microscopic (SEM) analyses of pGlcNAc. (D) Scanning electron microscopic (SEM) analyses of sNAG. [00281 Fig. 5. Wound closure analysis of sNAG (irradiated pGlcNAc, see Sections 5.3 and 6.2.1 below) membrane treated and untreated control wounds. Standardized photographs were taken on day 0, 4, 7, 10, 14, 17, 21, 25, and 28. Wound contraction (C), reepithelialization (E), and open wound (0) were studied as a percentage of the original wound area. [0029] Fig. 6-10. Macroscopic photograph of a wound of a sNAG membrane treated mice and a wound of an untreated control mice at day 0 (Fig. 6), 4 (Fig. 7), 10 (Fig. 8), 17 (Fig. 9), and 28 (Fig. 10). [00301 Fig. 11-15. sNAG induced faster wound closure. The application of the sNAG membrane induced faster reduction of raw surface (Fig. 11). The sNAG membrane treated mice reached 50% (Fig. 12) and 90% (Fig. 13) wound closure faster than the untreated control mice. The sNAG membrane treated mice showed enhanced reepithelialization on day 7 WO 2008/103345 PCTIUS2008/002172 4, 7 and 10 (Fig. 14). The sNAG membrane treated mice showered greater wound contraction on day 14 and 17 (Fig. 15). [00311 Fig. 16-17. Histology of the wound edge in a sNAG membrane treated mouse (Fig. 17) and an untreated control mouse (Fig. 16). [0032] Fig. 18. Ki-67 staining: the sNAG membrane treated mice showed enhanced cell proliferation on day 10 compared to the untreated control mice. [0033] Fig. 19. PECAM-1 staining: the sNAG membrane treated mice showed enhanced neo-vascularization on day 10 compared to the untreated control mice. [0034] Fig. 20. Degree of wound reepithelialization for sNAG membrane treated mice and untreated control mice measured at day 10. [0035] Fig. 21. Degree of granulation tissue formation for sNAG membrane treated mice and untreated control mice measured at day 10. [0036] Fig. 22. Histological analysis of untreated control vs. sNAG membrane treated wound at day 10. Note that there is no foreign body reaction in the sNAG membrane treated mice. [0037] Fig. 23. Collagen staining of untreated control vs. sNAG membrane treated wound at day 10. No foreign body encapsulations can be observed in the sNAG membrane treated mice. [0038] Fig. 24. pGlcNAc protected human umbilical vein endothelial cell (EC) from cell death induced by serum deprivation. For each time period (i.e., at 24, 48 and 72 hours), the identity for each of the five bars (from left to right) is as follows: serum starvation (SS), VEGF, and pGlcNAc (NAG) at 50, 100, and 250 sg/ml. [0039] Fig. 25. pGlcNAc did not effect metabolic rate. For each time period (i.e., at 24 and 48 hours), the identity for each of the four bars (from left to right) is as follows: serum starvation (SS), VEGF, and pGlcNAc (NAG) at 50 and 100 pg/ml. [0040] Fig. 26. pGlcNAc increased cell migration. [0041] Fig. 27. pGlcNAc caused an increase in migration toward fibronectin. Identity for each of the five bars (from left to right) is as follows: serum starvation (SS), VEGF, pGlcNAc (NAG) at 50 and 100 250 pg/ml, and NAG/VEGF. [00421 Fig. 28. pGlcNAc increased cord formation. 8 WO 2008/103345 PCTIUS2008/002172 [0043] Fig. 29. pGlcNAc induced effectors involved in cell motility (A) pGlcNAc treatment stimulated the expression of Ets 1, metallothionein 2A (MT), Akt3 and Edg3. (B) Real-time PCR demonstrates that Etsl was induced approximately 2-fold by pGlcNAc treatment. (C) Etsl increase in message was accompanied by a higher protein expression as shown in the Western blot analysis. [0044] Fig. 30. pGlcNAc's induction of phospho-MAPK was VEGFR2-dependent (A) pGlcNAc treatment resulted in a marked increase in the phosphorylation of MAPK. (B) pGlcNAc's induction of phosphor-MAPK was dependent on VEGFR2. [00451 Fig. 31. pGlcNAc did not activate VEGFR2. [0046] Fig. 32. pGlcNAc induced migration was Etsl dependent. (A) Inhibition of Etsl activity in EC resulted in a marked decrease in EC migration in response to pGlcNAc. (B) Ets1 protein expression decreased with the amount of dn-Ets construct. (C) The resultant expression levels of Ets1 in EC transfected with 2 amounts of plasmid-containing RNAi directed against Etsl. [0047] Fig. 33. pGlcNAc induced cell motility required integrin. (A) The results when using antibodies directed against aVp3 or aspi (CD49e) integrin in migration assays toward fibronectin (the aVp 3 receptor). (B) A similar experiment as in (A) using antibodies directed against UVp 3 or assi (CD49e) in transwells coated with vitronectin. [00481 Fig. 34. pGlcNAc-induced cell motility and activation of FAK via integrin engagement. [0049] Fig. 35. pGlcNAc can activate an integrin-+Etsl pathway leading to angiogenesis in a wound-healing model. (A) Antibody blockade of asp, integrin results in a reduction in pGlcNAc-induced Ets1 expression. (B) This inhibition of Etsl expression using a blockade of aspi integrin is recapitulated on the protein level. [0050] Fig. 36. pGlcNAc induced the expression of VEGF and IL-I. (A) pGlcNAc increased expression of both VEGF and IL-I. (B) Treatment of EC with its inhibitor blocked the induction of Etsl by VEGF but had no effect on the induction of Ets1 by pGlcNAc. [0051] Fig. 37. sNAG induced the expression of FGF1, FGFR3, Stabilin, IFNg, CollagenA18, and CXCL9. [0052] Fig. 38. sNAG increased cell migration. Identity for each of the four bars (from left to right) is as follows: serum starvation (SS), and sNAG at 50 and 100 pg/ml. 9 WO 2008/103345 PCT/US2008/002172 [00531 Fig. 39. sNAG induced marked increase in metabolic rate. Identity for each of the five bars (from left to right) is as follows: serum starvation (SS), VEGF, and sNAG at 50, 100 and 200 pg/ml. [0054] Fig. 40. sNAG did not protect EC from cell death induced by serum deprivation. For each time period (i.e., at 24 and 48 hours), the identity for each of the five bars (from left to right) is as follows: serum starvation (SS), VEGF, and sNAG at 50, 100 and 200 pLg/ml. [00551 Fig. 41. sNAG induced the expression of VEGF and IL-1. 5. DETAILED DESCRIPTION [0056] Sections 6.1 and 6.2 below clearly show that application of pGlcNAc, and in particular sNAG, to wounds in a diabetic mouse model for impaired wounds healing significantly shorten time for wound closure, wound reepithelialization, and wound contraction. Reepithelialization and granulation tissue were also enhanced in a shorter time for sNAG membrane treated mice. Application of the sNAG membrane also resulted in closure of all wounds in the treated animals in 28 days, compared to 85% in the untreated controls. Immunohistology data are consistent with the increased cell proliferation and appearance of new blood vessels (angiogenesis) in sNAG membrane treated mice. No foreign body response was observed throughout the study in any animal treated with the sNAG membrane. [00571 These findings suggest that the pGlcNAc and sNAG membrane have clinical applications in the treatment of chronic wounds including diabetic ulcers, venous stasis ulcers, arterial insufficiency ulcers, and pressure ulcers. The pGlcNAc and sNAG membrane also have clinical utilities in the treatment of other open wounds, such as, but not limited to, surgical wounds and burn wounds. 5.1 INJURIES FOR TREATMENT BY THE PRESENT METHODS AND COMPOSITIONS [0058] The methods and compositions described herein are useful for treating wounds such as open wounds caused by incision (i.e., incision or incised wound), laceration, abrasion, puncture, penetration, gunshot, burning, etc. The methods and compositions described herein are particularly useful for treating chronic wounds, or patients who fail to heal normally, or in an orderly or timely fashion as in other human subjects. 10 WO 2008/103345 PCT/US2008/002172 [0059] A chronic wound is a wound that has failed to proceed through an orderly and timely series of events to produce a durable structural, functional, and cosmetic closure. Chronic wounds can be any wound that fail to heal properly, including a surgical wound (e.g., a skin graft donor site), a cutaneous ulcer (e.g., a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, or a pressure ulcer), or a burn wound. [0060] Identifying the type of chronic wound being treated (e.g., diabetic, venous stasis, arterial insufficiency, and pressure) usually can be determined by considering the patient's history and performing a physical examination. Objective tools to confirm the diagnosis can include Doppler sonography to qualify and quantify vascular insufficiency: arterial or venous (deep, superficial, or mixed); transcutaneous oxygen tension (tcpO 2 ) measurements; ankle/brachial index; filament testing to quantify sensory neuropathy; measurement of laboratory markers for diabetes mellitus; histopathology of ulcer biopsies. In addition, there widely accepted criteria used to classify the stages of ulcers (e.g., National Pressure Ulcer Advisory Panel (NPUAP) for Pressure Ulcers: NPUAP Classification, Wagner's Classification for foot ulcers). Such methods are routinely practiced by the skilled artisan. [00611 Identifying a patient who fails to heal normally, or in an orderly or timely fashion as in other human subjects can routinely be determined by, for example, considering the patient's history and performing a physical examination. Such determinations are routinely made by the skilled artisan. 5.2 HEMOSTATIC COMPOSITION MATERIALS [00621 The hemostatic compositions are preferably formulated into wound dressings. The wound dressings can be made of any suitable natural or synthetic polymers or fibers. Examples of suitable polymers or fibers from which dressings can be prepared for practicing the methods and compositions described herein include cellulose polymers, xanthan, polyaramides, polyamides, polyimides, polyamide/imides, polyamidehydrazides, polyhydrazides, polyimidazoles, polybenzoxazoles, polyester/amide, polyester/imide, polycarbonate/amides, polycarbonate/imides, polysulfone/amides, polysulfone imides, and the like, copolymers and blends thereof. Other suitable classes of polymers or fibers include polyvinyledene fluorides and polyacrylonitriles. Examples of these polymers or fibers include those described in U.S. Patent Nos. RE 30,351; 4,705,540, 4,717,393; 4,717,394; 4,912,197; 4,838,900; 4,935,490; 4,851,505; 4,880,442; 4,863,496; 4,961,539; and European Patent Application 0 219 878, all of which are incorporated by reference. The polymers or 11 WO 2008/103345 PCT/US2008/002172 fibers can include at least one of either of cellulose polymers, polyamides, polyaramides, polyamide/imides or polyimides. In certain embodiments, the polymers or fibers include polyaramides, polyester, urethan and polytetrafluoroethylene. In preferred embodiments, polymerized N-acetylglucosamine fibers or derivatives thereof are used. In a most preferred embodiment, the polymer or fiber is poly-N-acetylglucosamine polymer or fiber or a derivative thereof. In certain embodiments, the poly-N-acetylglucosamine polymer or fiber has a p-1->4 configuration. In other embodiments, the poly-N-acetylglucosamine polymer or fiber has a a-1-+4 configuration. [0063] In specific embodiments, the polymer or fiber is chitin, chitosan, cellulose, nylon or PET (polyethylene terepthlate). [00641 In a preferred embodiment, the polymer or fiber is biocompatible and/or biodegradable. Biocompatibility may be determined by a variety of techniques, including, but not limited to such procedures as the elution test, intramuscular implantation, or intracutaneous or systemic injection into animal subjects. Such tests are described in U.S. Patent No. 6,686,342, which is incorporated by reference herein in its entirety. Biodegradable polymers preferably degrade within about I day, 2 day, 5 day, 8 day, 12 day, 17 day, 25 day, 30 day, 35 day, 40 day, 45 day, 50 day, 55 day, 60 day, 65 day, 70 day, 75 day, 80 day, 85 day, 90 day, 95 day, or 100 days after administration or implantation into a patient. [00651 In certain aspects, the polymer or fiber is immunoneutral, in that they do not elicit an immune response. [0066] Generally, the polymers or fibers are non-reactive in an intramuscular implantation test. In certain embodiments, the compositions comprise fibers as described in this section, wherein the fibers increases metabolic rate of serum-starved human umbilical cord vein endothelial cells in a MTT assay and/or does not rescue apoptosis of serum-starved human umbilical cord vein endothelial cells in a trypan blue exclusion test, and is non reactive when tested in an intramuscular implantation test. In certain embodiments, the fibers increases metabolic rate of serum-starved human umbilical cord vein endothelial cells in a MTT assay and does not rescue apoptosis of serum-starved human umbilical cord vein endothelial cells in a trypan blue exclusion test, and is non-reactive when tested in an intramuscular implantation test 12 WO 2008/103345 PCT/US2008/002172 [00671 In one embodiment, the hemostatic compositions comprise purified polymers or fibers, which may be about 100%, 99.9%, 99.8%, 99.5%, 99%, 98%, 95%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, or 20% pure. In a preferred embodiment, the polymers or fibers used in the compositions and methods described herein are 90-100% pure. [0068] In certain embodiments, the polymer or fiber that is used in a wound dressing is not one or more of the following: an ionic synthetic hydrogel such as, but not limited to, crosslinked poly(AAn-acrylic acid) and poly(AAm-dimethylaminoethyl methacrylate), poly(vinyl alcohol) (PVA), poly(ethylene glycol) (PEG), poly(N-vynl pyrrolidone), poly(methoxy-PEG methacrylate). In certain embodiments, the polymer or fiber is not one or more of: a poly-L-amino acid, such as poly-L-lysine, poly-L-arginine, poly-L-glutamic acid, poly-L-histidine, poly-D-glutamic acid or a mixture thereof. In certain embodiments, the polymer or fiber is not one or more of the following: an alginate polymer, such as sodium alginate, calcium alginate, strontium alginate, barium alginate, magnesium alginate or any other alginate or a mixture thereof. In certain embodiments, the polymer or fiber is not derived from one or more of the following: a shell fish, a crustacean, insect, fungi or yeasts. In certain embodiments, the compositions do not comprise collagen fibers. In certain embodiments, the compositions do not comprise elastin fibers. In other embodiments, these polymers or fibers are included in the compositions. [0069] The polymers described herein are generally in the form of fibers comprising, polymers described herein. Thus, the polymers described herein can be in the form of fibers. Fibers are preferably about 2, 3, 4, 5, 8, 10, 20, 30, 40, 50, 60 , 70, 80, 90, 100, 110, 120, 130, 140 or 150 microns in average length as determined by electron microscopy, or any range in between (e.g., 10-150 microns or 20-100 microns or 50-120 microns on average). The fibers are preferably nanofibers, with dimensions averaging 0.5-100 rim in thickness and/or diameter as determined by electron microscopy. In specific embodiments, the nanofibers are about 0.5, 1, 2, 5, 10, 20, 50 or 100 rm in thickness and/or diameter on average, or any range in between (e.g., 0.5-4 rim, 0.5-5 nm, 1-4 nm, 1-5 rm, 1-10 nm, 2-20 rim, 4-15 nm, 5-15 nm, 4-20 rim, 5-20 rim, 5-50 nm, etc.). In other embodiments, the fibers are preferably microfibers, with dimensions of about 0.20, 0.25, 0.30, 0.35, 0.40, 0.45, 0.50, 0.55, 0.60, 0.65, 0.75, 0.85 and 1 micron in thickness and/or diameter on average, or any range in between (e.g., 0.2-0.5 micron, 0.3-0.75 micron, 0.5-1 micron, and so on and so forth). As used herein, the metes and bound of the term "about" would readily be appreciated by those 13 WO 2008/103345 PCT/US2008/002172 of skill in the art; generally, the term as used herein refers to a range of values 10% greater than and 10% less than the stated value. [0070] In specific embodiments, the majority (greater than 50%, e.g., 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, 99.9%, or 100%) of the fibers are less than about 30, 25, 20, 15, 10, 5, 4, or 3 microns in length. In specific embodiments, at least 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, or 99.9% of the fibers are less than about 15 microns in length. In specific embodiments, all (100%) of the fibers are less than about 15 microns in length. In specific embodiments, the majority (greater than 50%, e.g., 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, 99.9%, or 100%) of the fibers are about 5, 4, 3, 2, or 1 microns in length. In specific embodiments, at least 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, or 99.9% of the fibers are less than about 4 microns in length. In specific embodiments, all (100%) of the fibers are about 4 microns in length. [0071] Fibers are preferably about 0.1, 0.5, 1, 2, 3, 4, 5, 8, or 10 microns in average thickness or diameter as determined by electron microscopy, or any range in between (e.g., 0.1-10 microns or 0.5-5 microns or 1-2 microns on average). In specific embodiments, the majority (greater than 50%, e.g., 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, 99.9%, or 100%) of the fibers have a thickness or diameter of about 1-2 microns. In specific embodiments, at least 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, or 99.9% of the fibers have a thickness or diameter of about 1-2 microns. In specific embodiments, all (100%) of the fibers have a thickness or diameter of about 1-2 microns. [0072] In certain embodiments, the majority (greater than 50%, e.g., 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, 99.9%, or 100%) of the fibers are less than about 15 microns in length and have a thickness or diameter of about 1-2 microns. [0073] In certain preferred embodiments, the polymers or fibers are formulated as wound dressings, which can be in the form of barriers, membranes, or films. Alternatively, the polymers or fibers are added to dressing backings, such as barriers, membranes, or films. A barrier, membrane, or film can be supplied in a variety of standard sizes, which can be further cut and sized to the area being treated. The backing can be a conventional dressing material, such as a bandage or gauze to which a polymer or fiber is added or coated on, prior to application to the patient. Alternatively, the polymer or fiber can be formulated as a barrier, membrane, or film made out of strings, microbeads, microspheres, or microfibrils, or the composition can be formulated as a barrier-forming mat. As pointed out herein, the polymers 14 WO 2008/103345 PCT/US2008/002172 can be present in the form of fibers. As such, throughout the specification, whether or not explicitly recited, it is understood that any emobdiments referring to polymers described herein, the polymers can be present in the form of fibers. [00741 In certain embodiments, at least 75%, at least 85%, at least 90%, or at least 95% of a dressing is composed of one or more of the polymers listed above. [0075] In certain aspects, a dressing does not contain a conventional dressing material such as a gauze or bandage. In such embodiments, the polymer itself if formulated as the wound dressing. [0076] In one embodiment, the compositions described herein comprise more than one type of polymer (e.g., poly-@-1->4-N-acetylglucosamine and cellulose). [0077] In certain aspects, the polymer (e.g., the poly-p-I ->4-N-acetylglucosamine or derivative thereof) is the only active ingredient in a dressing. In other embodiments, the dressing comprises additional active ingredients to promote wound healing, such as growth factors. In specific embodiments, the growth factor is PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, PDGF-DD, FGF-1, FGF-2, FGF-5, FGF-7, FGF-10, EGF, TGF-a, (HB-EGF), amphiregulin, epiregulin, betacellulin, neuregulins, epigen, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, placenta growth factor (PLGF), angiopoietin-1, angiopoietin -2, IGF-I , IGF-II, hepatocyte growth factor (HGF), or macrophage-stimulating protein (MSP). [0078] However, in other aspects, a dressing does not comprise a significant amount of protein material. In specific embodiments, the protein content of a dressing is no greater than 0.1%, 0.5% or 1% by weight. In other embodiments, the protein content of a dressing is undetectable by Coomassie staining. [0079] A dressing may contain collagen, although in certain aspects a dressing does not contain collagen. [0080] The dressing may also contain antimicrobial agents to prevent infection of the wounds. [0081] In a preferred embodiment, zinc is also included in a dressing. In addition to its antimicrobial properties, zinc also plays a role in wound healing (see Andrews et al., 1999, Adv Wound Care 12:137-8). The zinc is preferably added in the form of a salt, such as zinc oxide, zinc sulphate, zinc acetate or zinc gluconate. 15 WO 2008/103345 PCT/US2008/002172 5.2.1 Poly-p-1-+4-N-acetylgulcosamine ("pGlcNAc" or "NAG") 100821 This section incorporates by reference U.S. Patent Nos. 5,622,834; 5,623,064; 5,624,679; 5,686,115; 5,858,350; 6,599,720; 6,686,342; and 7,115,588 that describe in detail the structure of poly-p-I->4-N-acetylglucosamine polymer that is a preferred embodiment, each of which is incorporated by reference herein in its entirety. [0083] In preferred embodiments, poly-p-I-+4-N-acetylglucosamine is derived from a process comprising a) treating a microalga comprising a cell body and a poly-$0-1->4-N acetylglucosamine polymer fiber with a biological agent (such as hydofluoric) capable of separating the N-acetylglucosamine polymer fiber from the cell body for a sufficient time so that the poly-p- 1 -+4-N-acetylglucosamine polymer fiber is released from the cell body; b) segregating the poly-p-1-+4-N-acetylglucosamine polymer fiber from the cell body; and c) removing contaminants from the segregated poly-p-I1-+4-N-acetylglucosamine polymer fiber, so that the poly-p-1->4-N-acetylglucosamine polymer is isolated and purified. [0084] As used herein derivatives of a poly-p-1--4-N-acetylglucosamine polymer include: a semi-crystalline form of a poly-p-1->4-N-acetylglucosamine polymer; a poly-p 1->4-N-acetylglucosamine polymer comprising about 50 to about 150,000 N acetylglucosamine monosaccharides covalently attached in a 0-1->-4 conformation, and said polymer has a molecular weight of about 10,000 daltons to about 30 million daltons; a poly p-1--+4-N-acetylglucosamine polymer comprising about 50 to about 50,000 N acetylglucosamine monosaccharides covalently attached in a 0-1-+4 conformation, and said polymer has a molecular weight of about 10,000 daltons to about 10 million daltons; a poly p-1-+4-N-acetylglucosamine polymer comprises about 50 to about 10,000 N acetylglucosamine monosaccharides covalently attached in a p-1-->4 conformation, and said polymer has a molecular weight of about 10,000 daltons to about 2 million daltons; a poly-$ 1--+4-N-acetylglucosamine polymer comprising about 50 to about 4,000 N acetylglucosamine monosaccharides covalently attached in a p-1 -- >4 conformation, and said polymer has a molecular weight of about 10,000 daltons to about 800,000 daltons; a semi crystalline poly-p-1->4-N-acetylglucosamine polymer comprising at least one N acetylglucosamine monosaccharide that is deacetylated, and wherein at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% of said N-acetylglucosamine monosaccharides are acetylated; and a semi-crystalline poly-0-1-+4-N-acetylglucosamine polymer comprising at least one N-acetylglucosamine monosaccharide that is deacetylated, and wherein at least 16 WO 2008/103345 PCT/US2008/002172 20-70% (or any range in between the foregoing embodiments) of said N-acetylglucosamine monosaccharides are deacetylated. [0085] Derivatives of a poly-p-1--+4-N-acetylglucosamine polymer also include compositions that are 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% or less poly p-1-+4-N-acetylglucosamine. [0086] Other derivatives of poly-p-1--+4-N-acetylglucosamine may also be used in the composition described herein. For example, sulfated poly- -1--+4-N-acetylglucosamine derivatives, phosphorylated poly-p-1-+4-N-acetylglucosamine derivatives, or nitrated poly-p 1->4-N-acetylglucosamine derivatives may be used. Additionally, one or more of the monosaccharide units of the poly-p-1-+4-N-acetylglucosamine may contain one or more sulfonyl groups one or more O-acyl groups. In addition, one or more of the monosaccharides of the deacetylated poly-p-1--+4-N-acetylglucosamine may contain an N-acyl group. One or more of the monosaccharides of the poly-- 1 -- +4-N-acetylglucosamine or of its deacetylated derivative, may contain an O-alkyl group. One or more of the monosaccharide units of the poly-p-1-->4-N-acetylglucosamine may be an alkali derivative. One or more of the monosaccharide units of the deacetylated derivative of poly-p-1-+4-N-acetylglucosamine may contain an N-alkyl group. One or more of the monosaccharide units of the deacetylated derivative of poly-p-1-+4-N-acetylglucosamine may contain at least one deoxyhalogen derivative. One or more of the monosaccharide units of the deacetylated derivative of poly p-I-+4-N-acetylglucosamine may form a salt. One or more of the monosaccharide units of the deacetylated derivative of poly-p-1--+4-N-acetylglucosamine may form a metal chelate. Preferably, the metal is zinc. One or more of the monosaccharide units of the deacetylated derivative of poly-p-1-4-N-acetylglucosamine may contain an N-alkylidene or an N arylidene group. Methods of making such derivatives are described in U.S. Patent No. 5,623,064, which is incorporated by reference herein in its entirety. 5.3 IRRADIATION TO REDUCE MOLECULAR WEIGHT AND LENGTH [0087] The polymers or fibers can be in irradiated as dry polymers or fibers or polymer or fiber membranes, as described above. Alternatively, the polymers or fibers can be irradiated when wet. [0088] In preferred embodiments, the polymers or fibers are formulated into a suspension/slurry or wet cake for irradiation. Irradiation can be performed prior to, concurrently with or following the formulation of the polymers or fibers into a dressing. 17 WO 2008/103345 PCT/US2008/002172 Generally, the polymer or fiber content of suspensions/slurries and wet cakes can vary, for example from about 0.5 mg to about 50 mg of polymer or fiber per I ml of distilled water are used for slurries and from about 50 mg to about 1000 mg of polymer or fiber per I ml of distilled water are use for wet cake formulations. The polymer or fiber may first be lyophilized, frozen in liquid nitrogen, and pulverized, to make it more susceptible to forming a suspension/slurry or wet cake. Also, the suspensions/slurries can be filtered to remove water such that a wet cake is formed. In certain aspects, the polymer or fiber is irradiated as a suspension comprising about 0.5 mg, 1 mg, 2 mg, 3 mg, 4 mg, 5 mg, 6 mg, 7 mg, 8 mg, 9 mg, 10 mg, 12 mg, 15 mg, 18 mg, 20 mg, 25 mg or 50 mg of polymer or fiber per ml of distilled water, or any range in between the foregoing embodiments (e.g., 1-10 mg/ml, 5-15 mg/ml, 2-8 mg/ml, 20-50 mg/ml, etc.). In other aspects, the polymer or fiber is irradiated as a wet cake, comprising about 50-1,000 mg polymer or fiber per 1 ml of distilled water. In specific embodiments, the wet cake comprises about 50, 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000 mg of polymer or fiber per 1 ml distilled water, or any range in between (e.g., 100-500 mg/ml, 300-600 mg/ml, 50-1000 mg/ml, etc.). [0089] The irradiation is preferably in the form of gamma radiation, e-beam radiation, or x-rays. Two sources of irradiation are preferred: radioactive nuclides and electricity. In specific embodiment, the radioactive nuclides are cobalt-60 and cesium-137. Both of these nuclides emit gamma rays, which are photons containing no mass. The gamma rays have energies from 0.66 to 1.3 MeV. Using electricity, electrons are generated and accelerated to energies up to 10 MeV or higher. When irradiating polymers or fibers to reduce their size, a consideration to take into account is that the depth of penetration of materials with densities similar to water by 10 MeV electrons is limited to about 3.7 cm with one-sided exposure or about 8.6 cm with two-sided exposure. Depth of penetration decreases at lower electron energies. Electron energy can be converted to x-rays by placing a metal (usually tungsten or tantalum) target in the electron beam path. Conversion to x-rays is limited to electrons with energies up to 5 MeV. X-rays are photons with no mass and can penetrate polymers or fibers similar to gamma rays. There is only about 8% efficiency in the conversion of electron energy to x-ray energy. High powered electron beam machines are needed in x-ray production facilities to account for the low conversion efficiency. [00901 Preferably, the irradiation is gamma irradiation. [0091] The absorbed dose of radiation is the energy absorbed per unit weight of product, measured in gray (gy) or kilogray (kgy). For dried polymers or fibers, the preferred absorbed 18 WO 2008/103345 PCT/US2008/002172 dose is 500-2,000 kgy of radiation, most preferably 750-1,250 kgy of radiation, while for wet polymers or fibers, the preferred absorbed dose is about 100-500 kgy of radiation, most preferably about 150-250 kgy of radiation. [00921 The dose of radiation can be described in terms of its effect on the length of the polymers or fibers. In specific embodiments, the dose of radiation used preferably reduces the length of the polymer or fiber by anywhere from about 10% to 90% of the starting length of the polymer or fiber, respectively. In specific embodiments, the average length is reduced by about 10%, by about 20%, by about 30%, by about 40%, by about 50%, by about 60%, by about 70%, by about 80%, or by about 90%, or any range in between (e.g., 20-40%, 30-70%, and so on and so forth). Alternatively, the dose of radiation used preferably reduces the length of the polymer or fiber to anywhere from 30 to 100 microns. In specific embodiments, and depending on the starting fiber length, the average length of the polymer or fiber is reduced to less than about 15 microns, less than about 14 microns, less than about 13 microns, less than about 12 microns, less than about 11 microns, less than about 10 microns, less than about 5 microns, less than about 4 microns, less than about 3 microns, less than 2 microns, or less than 1 microns. In certain embodiments, the length of the majority (greater than 50%, e.g., 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, 99.9%, or 100%) of the polymers or fibers is reduced to no greater than 40 microns, no greater than about 30 microns, no greater than about 20 microns, no greater than about 15 microns, no greater than about 10 microns, or no greater than about 5 microns. Any ranges between the foregoing length are also encompassed; for example, in certain embodiments, irradiation of the polymers or fibers reduces the length of the majority (greater than 50%, e.g., 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, 99.9%, or 100%) of the fibers to anywhere between about I to 20 microns, between about 2 to 15 microns, between about 4 to 10 microns, and so on and so forth. [0093] The dose of radiation can also be described in terms of its effect on the molecular weight of the polymer or fiber. In specific embodiments, the dose of radiation used preferably reduces the molecular weight of the polymer or fiber by anywhere from about 10% to 90% of the starting weight of the polymer or fiber. In specific embodiments, the average molecular weight is reduced by about 10%, by about 20%, by about 30%, by about 40%, by about 50%, by about 60%, by about 70%, by about 80%, or by about 90%, or any range in between (e.g., 20-40%, 30-70%, and so on and so forth). Alternatively, the dose of radiation used preferably reduces the molecular weight of the polymer or fiber to anywhere from 1,000 19 WO 2008/103345 PCT/US2008/002172 to 1,000,000 daltons. In specific embodiments, and depending on the starting molecular weight, the average molecular weight of the polymer or fiber is reduced to less than 1,000,000 daltons, less than 750,000 daltons, less than 500,000 daltons, less than 300,000 daltons, less than 200,000 daltons, less than 100,000 daltons, less than 50,000 daltons, less than 25,000 daltons, less than 10,000 daltons, or less than 5,000 daltons. In certain embodiments, the average molecular weight is reduced to no less than 500 daltons, no less than 1,000 daltons, no less than 2000 daltons, no less 3,500 daltons, no less than 5,000 daltons, no less than 7,5000 daltons, no less than 10,000 daltons, no less than 25,000 daltons, no less than 50,000 daltons, or no less than 100,000 daltons. Any ranges between the foregoing average molecular weights are also encompassed; for example, in certain embodiments, irradiation of the polymer or fiber reduces the average molecular weight to anywhere between 10,000 to 100,000 daltons, between 1,000 and 25,000 daltons, between 50,000 and 500,000 daltons, and so on and so forth. [00941 In preferred embodiments, the irradiation used is gamma irradiation. [00951 Following irradiation, slurries can be filtered and dried, and wet cakes can be dried, to form dressings that are useful in the practice described herein. 5.3.1 Shortened poly-p-1--+4-N-acetylgulcosamine ("sNAG") [0096] The poly-p-1-.4-N-acetylgulcosamine as described in Section 5.2 above can be subject to irradiation as described in Section 5.3 above to reduce the length of its fibers to form shortened poly-p-1-+4-N-acetylgulcosamine, and thus reducing its molecular weight without disturbing its microstructure. The infrared spectrum (IR) of the shortened poly-p 1->4-N-acetylgulcosamine (sNAG) is substantially similar or equivalent to that of the non irradiated poly-p-1-4-N-acetylgulcosamine (NAG). [0097] In certain embodiments, the majority (greater than 50%, e.g., 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.5%, 99.8%, 99.9%, or 100%) of the fibers of sNAG is less than 15 microns in length. In one aspect, the majority of the fibers of sNAG have a thickness or diameter of about 1-2 microns. The length of the fibers can be measured by any method known to one skilled in the art, for example, by scanning electron microscopy (SEM). [0098] In one aspect, sNAG increases the metabolic rate of serum-starved human umbilical cord vein endothelial cell (EC) in a MTT assay. A MTT assay is a laboratory test and a standard colorimetric assay (an assay which measures changes in color) for measuring cellular proliferation (cell growth). Briefly, yellow MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5 20 WO 2008/103345 PCT/US2008/002172 diphenyltetrazolium bromide, a tetrazole) is reduced to purple formazan in the mitochondria of living cells. This reduction takes place only when mitochondrial reductase enzymes are active, and therefore conversion can be directly related to the number of viable (living) cells. The metabolic rate of cells may be determined by other techniques commonly known to the skilled artisan. [00991 In another aspect, sNAG does not rescue apoptosis of serum-starved EC in a trypan blue exclusion test. A trypan blue exclusion test is a dye exclusion test used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. The viability of cells may be determined by other techniques commonly known to the skilled artisan. [00100] In another aspect, sNAG is non-reactive when tested in an intramuscular implantation test. In one aspect, an intramuscular implantation test is an intramuscular implantation test - ISO 4 week implantation, as described in Section 6.4.2 below. [001011 In certain embodiments, sNAG comprises fibers, wherein the majority of the fibers are less than about 15 microns in length, and is non-reactive when tested in an intramuscular implantation test. [001021 In certain embodiments, sNAG (i) comprises fibers, wherein the majority of the fibers are less than about 15 microns in length, and (ii) (a) increases the metabolic rate of serum-starved EC in a MTT assay and/or does not rescue apoptosis of serum-starved EC in a trypan blue exclusion test, and (b) is non-reactive when tested in an intramuscular implantation test. 5.4 METHODS FOR USING THE HEMOSTATIC COMPOSITIONS [001031 Described herein are methods for treating a wound in patients. The methods generally comprise applying a dressing to a wound in a patient, wherein the dressing comprises or consists of any of the polymers or fibers listed in Sections 5.2 and 5.3 above. [00104] The patient is preferably a mammal, most preferably a human. [00105] In certain embodiments, the patient is an adult, an adolescent, or an infant. In one embodiment, the patient is a human older than age 45. In one embodiment, the patient is a woman who is not pregnant. 21 WO 2008/103345 PCT/US2008/002172 [00106] In certain embodiments, the mammal is a livestock mammal (e.g., cow or sheep or pig) or household pet (e.g., cat or dog). [00107] In certain embodiments, methods are described herein for treating a wound in patients who do not normally heal in an orderly or timely fashion as other human subjects. The patient is preferably a human such as a diabetic, a smoker, a hemophiliac, an HIV infected person, an obese person, a person undergoing radiation therapy, or a person with venous stasis ulcer. In one aspect, the patient is a person with venous stasis ulcer. In certain aspects, the wound is a surgical wound or a bum wound. In certain other aspects, the wound is a chronic wound such as a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, or a pressure ulcer. [001081 In certain embodiments, methods are described herein for treating a chronic wound in patients, wherein the chronic wound does not heal in an orderly or timely fashion. In certain embodiments, the wound is a chronic wound such as a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, or a pressure ulcer. In one aspect, the chronic wound is a venous stasis ulcer. [00109] The hemostatic compositions and methods described herein advantageously reduces the labor and cost of nursing care associated with wounds, especially chronic wounds. Wound dressings currently available on the market require changing every approximately two days; in contrast, in the present methods the wound dressings are changed or reapplied every 3-35 days. In specific embodiments, the wound dressings are changed or reapplied every 4-35 days, every 5-35 days, every 6-35 days or every 7-35 days. In certain aspect, the wound dressings are changed or reapplied every 3-10 days, every 4-14 days, every 5-12 days, every 7-14 days, every 7-28 days, every 7-21 days, every 14-28 days, or any range between 3, 4, 5, 6, or 7 days at the lower end to every 8, 9, 10, 12, 14, 18, 21, 24, 28, 30 or 35 days on the higher end. In one aspect, the wound dressings are changed or reapplied only one time at the beginning of treatment. In another aspect, the wound dressings are changed or reapplied once per week during treatment. In another aspect, the wound dressings are changed or reapplied every other week during treatment. In another aspect, the wound dressings are changed or reapplied every two weeks, three weeks, or four weeks during treatment. The present methods reduce the frequency of wound dressing changes by at least 50%, and can reduce the frequency of wound dressing changes by 100%, 200%, 500% or even more. The specific frequency for changing or reapplying the wound dressings will vary depending on the needs of the individual; i.e., depending on the size, classification and 22 WO 2008/103345 PCT/US2008/002172 responsiveness of the wound to the dressing. The frequency can be determined by standard clinical techniques, and need not be at fixed intervals. Rather, the frequency can be varied over time, depending on the needs of the individual. For example, as a chronic wound begins to heal, the frequency of wound dressing changes or reapplications are reduced. [001101 In certain aspects, the wound dressing is applied to the wound, where it may remain until it is removed or biodegrades. More preferably, the wound dressing is reapplied (without removing the previous wound dressing) or changed (by removing the previous wound dressing and applying a new dressing) at least once, but can be reapplied or changed at the frequencies described above for the duration of the wound. Preferably, the wound dressing is reapplied or changed at least twice, at least three times, at least four times or more. The therapeutic regimens can be performed over a period of a few weeks (e.g., 2-8 weeks) to several months (e.g., from 2-4 months up to 6-12 months or longer, and any range in between). [001111 Advantageously, the dressings also reduce overall wound healing time. For example, in the diabetic mouse model described in Section 6 below, the pGlcNAc dressing reduced the time to achieve wound closure one week faster than in control mice. Thus, in addition to reducing the number of dressing changes for a given wound, the methods described herein are useful for reducing wound closure time. Accordingly, in certain aspects, methods for reducing the wound closure time of a chronic wound are provided, comprising topically applying a dressing to a chronic wound in a patient, wherein the dressing comprises or is composed of one or more of the polymers listed in Sections 5.2 and 5.3 above. Optionally, the application is repeated one or more times in accordance with the therapeutic regimens described in this section. In certain aspects, the wound closure time is reduced by at least 10%, by at least 20 %, by at least 30%, by at least 40 % or by at least 50%. [001121 Most preferably, the polymer from which a dressing is made is a biocompatible and/or immunoneutral polymer, including but not limited to poly-p-1-+4-N acetylglucosamine or a derivative thereof. In a preferred embodiment, the poly--p-1->4-N acetylglucosamine or a derivative thereof is a microalgal poly-p-1-+4-N-acetylglucosamine or a derivative thereof. In certain aspects, the poly-p-l-+4-N-acetylglucosamine or a derivative thereof is not from a shellfish or crustacean source. [001131 The aforementioned methods can be used in conjunction with other standard care procedures for chronic wounds. For example, the methods can be used in conjunction with 23 WO 2008/103345 PCT/US2008/002172 one or more of the following treatments for cutaneous ulcer therapy: removal of necrotic or infected tissue (debridement); off-loading; compression therapy for venous stasis ulcers; establishment of adequate blood circulation; maintenance of a moist wound environment; management of wound infection; wound cleansing; nutritional support, including blood glucose control for subjects with diabetic ulcers; bowel and bladder care for subjects with pressure ulcers at risk for contamination. [00114] For burn wounds, standard care procedures that may be used in conjunction with the methods described herein include: hemodynamic resuscitation; management of co morbidities; timely burn debridement and excision; wound closure; management of wound infection; pain control; nutritional support; measures to inhibit excessive scar formation; and rehabilitation, including passive range of motion when burns overlie joints. 5.5 KITS [00115] A kit is also provided which comprises any of the above described dressing materials. The dressing is preferably contained within a sealed, water proof, sterile package which facilitates removal of the composition without contamination. Materials from which containers may be made include aluminum foil, plastic, or another conventional material that is easily sterilized. The kit can contain a single dressing or multiple dressings, preferably wherein each is provided in a separate, waterproof, sterile package. The dressing may further comprise wound healing agents or antimicrobials, as described in Sections 5.2 and 5.3 above. [001161 In another embodiment, a container having dual compartments is provided. A first compartment contains the dressing, while the second compartment contains an active agent such as a growth factor or antimicrobial agent. In the field or the clinic, the dressing can be readily dipped into the active agent subsequently applied to the wound. [00117] A kit can comprise a notice regarding FDA approval and/or instructions for use. [00118] Additionally, a kit designed for emergency or military use can also contain disposable pre-sterilized instruments, such as scissors, scalpel, clamp, tourniquet, elastic or inelastic bandages, or the like. 24 WO 2008/103345 PCT/US2008/002172 6. EXAMPLES 6.1 Example L Effects of poly-N-acetylglucosamine (pGlcNAc) patch on wound healing in db/db mouse. 6.1.1 Materials and Methods [001191 Preparation ofpGlcNAc Patch. The pGlcNAc patch SyvekPatchTM (Marine Polymer Technologies, Inc., Danvers, MA) consists of microalgal-derived nanofibers produced as previously described (see Vournakis et al. U.S. Patent Nos. 5,623,064; and 5,624,679, the content of each of which is incorporated herein by reference in its entirety). Briefly, microalgae were cultured in unique bioreactor conditions using a defined growth media. Following the harvest of microalgae from high-density cultures, nanofibers were isolated via a stepwise separation and purification process resulting in batches of pure nanofibers suspended in water for injections (wfi). Fibers were formulated into patches by concentration and oven drying, and were packaged and sterilized by gamma-irradiation. Nanofiber dimensions average 20-50nm x 1-2nm x 1 00pm. Batches of fibers were individually quality controlled using chemical and physical test parameters, and each batch met strict purity criteria prior to release. Final batches were required to be substantially free of proteins, metal ions, and other components. [001201 Wound Model and Study Design. Homozygous, genetically diabetic 8-12 week old, Lep/r - db/db male mice (strain C57BL/KsJ-Lepr d) were used under an approved animal protocol in an Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) accredited facility. The day before surgery, hair was clipped and depilated (Nair@, Church & Dwight Co., Princeton, NJ). On the day of the surgery, animals were weighed and anesthetized with 60 mg/kg Nembutal (Pentobarbital). A dorsal 1.0 cm 2 area of skin and panniculus carnosus was excised and the wounds were photographed. Wounds were covered with the pGlcNAc patch for 1 hour (1h group, n=15), 24 hrs (24h group, n=15), or left untreated (NT group, n=15). All wounds were covered with semi occlusive polyurethane dressings (Tegaderm@, 3M, St. Paul, MN). At day 10 and 21, 7-8 animals per group were euthanized and the wounds were photographed, excised, and fixed in 10% neutral-buffered formalin solution. N=15 per group were observed from day 1 to 10, and n=7-8 per group were observed from day 14 to day 21. [00121] Wound Closure Analysis. Three blinded independent observers compared digital photographs captured twice a week with initial photographs at day 0 using planimetric 25 WO 2008/103345 PCT/US2008/002172 methods. Wound closure was quantified by measuring contraction (C), reepithelialization (E), and open wound (0) as a percentage of the original wound area. The sum of contracted, reepithelialized, and open wound areas equals 100% of the original wound size (Fig. 1) (see Yannas I. Tissue and Organ Regeneration in adults. New York: Springer; 2001). [001221 Central wound cross-sections were embedded in paraffin, sectioned, and stained according to routine Hematoxylin and Eosin (H&E) protocols. Panoramic cross-sectional digital images of each wound were prepared using Adobe Photoshop CS Software (Adobe Systems Incorporated, San Jose, CA) in order to analyze granulation tissue area and thickness with digital planimetry (Image J, NIH, Bethesda, MD). [001231 Immunohistochemistry. Paraffin-embedded sections were re-hydrated and antigen retrieval for the immuno-histochemical marker of proliferation Ki-67 was accomplished by microwaving in 10 mM sodium citrate (pH 6.0) for ten minutes. Frozen sections were fixed with acetone and stained for the immuno-histochemical marker of vascularization platelet endothelial cell adhesion molecule one (PECAM-1). Ki-67 (LabVision, Freemont, Ca) primary antibody was incubated for 1 hour at room temperature, while PECAM-1 (Pharmingen, San Jose, CA) primary antibody was incubated at 4*C overnight. PECAM-1 signal was intensified using tyramide amplification system (Perkin Elmer, Boston, MA). [001241 Vessel Density Quantification. Digital color images of the wound sections were preprocessed before quantification to ensure uniform contrast of PECAM-1 positive areas relative to the background. A mask of positive staining was created using the color mask function of the program Corel PhotoPaint v.10 (Corel Corporation, Ottawa, Ontario, Canada) by sampling five different chromogen color tones represented in positively stained areas. The masked vessel areas were converted to pure black while the background was made pure white. The black and white representations were used for area quantification in IPLab software (BD Biosciences Bioimaging, Rockville, MD) by applying the segmentation function. Tissue regions were defined by projecting the original H&E image over the processed image. Blood vessel density, quantified over the entire image, was expressed as the ratio of vessel area to total granulation tissue area. Between 4 and 7 microscopic fields (20x) were used to evaluate vessel density for each wound and treatment modality. [00125] Quantification of Cell Proliferation. Wounds were analyzed for cell proliferation using image analysis of Ki-67-stained sections in a manner similar to the method of vessel density quantification. High-power digital images of Ki-67 stained wound sections were 26 WO 2008/103345 PCT/US2008/002172 used to measure the number of Ki-67 positive cells relative to the total number of nuclei. The degree of proliferation was quantified over the entire wound section using 4-6 fields at 20x magnification and expressed as a ratio of proliferating nuclei (Ki-67-positive) to total nuclei. [001261 Statistical Analysis. Values were expressed as means ± standard deviation in the text and figures. One-way analysis of variance and ad hoc LSD tests were used to determine the significance of differences between treatment modes. Multivariate analysis was performed using Statistica v7.0 (StatSoft, Inc, Tulsa, OK). 6.1.2 Results [001271 Statistical Analysis. Values were expressed as means ± standard deviation in the text and figures. One-way analysis of variance and ad hoc LSD tests were used to determine the significance of differences between treatment modes. Multivariate analysis was performed using Statistica v7.0 (StatSoft, Inc, Tulsa, OK). 1001281 pGlcNAc Patch Altered Wound Healing Kinetics. Treatment with pGlcNAc induced faster wound closure over time compared to non-treatment. The lh group showed faster (p<0.01) decrease of raw surface (open portion of the wound), compared to the NT group on day 7, 14 and 17 (Fig. 2A). The lh group also reached 90% closure on average in 16.6 days, which is nine days faster (p<0.01) than the NT group (25.6 days) (Fig. 2B). The 24h group reached 90% closure in 18.2 days (Fig. 2B). [001291 The lh group showed augmented (p<0.01) reepithelialization, compared to the NT group on day 4, 7, 14 and 21 (Fig. 2C, 2D). The 24h group showed increased (p<0.01) reepithelialization, compared to the NT group on day 4 and 21 (Fig. 2C). [001301 The 24h group showed decreased (p<0.01) contraction, compared to the NT group on day 4 and 17; and decreased (p<0.01) contraction, compared to the lh group on day 7 (Fig. 2E). The lh group showed decreased (p<0.01) contraction, compared to the NT group on day 14 (Fig. 2E). 100131] pGlcNAc Patch Increased Blood Vessel Density and Proliferation. One cm 2 , full thickness, untreated wounds in db/db mouse reached 50% closure between 8-12 days post operation (Chan et al. Effect of recombinant platelet-derived growth factor (Regranex) on wound closure in genetically diabetic mice. JBurn Care Res 2006;27(2):202-5). Day 10 was chosen as an intermediate time point for staging wound healing following the treatments. 27 WO 2008/103345 PCT/US2008/002172 1001321 Proliferating cells were stained for Ki-67 (Fig. 3A). PECAM-1 (CD3 1) was selected to stain endothelial cells and quantify the blood vessel density in granulation tissue (Fig. 3B). The lh group showed significant increases in blood vessel density and cell proliferation, compared to both the NT and 24h groups (Fig. 3A, 3B). PECAM-l and Ki-67 results were plotted together give visual correlation of angiogenesis and proliferation among different treatments (Fig. 3C). [001331 Granulation tissue area and thickness were measured in microphotographs at 4X magnification (n=7-8 per group) to assess the level of coverage by the newly formed tissue in response to injury and treatment modality, as shown below: Granulation tissue area mean d Granulation tissue thickness mean standard deviation standard deviation (pixels) (pixels) NT 5.5x10 5 ±5.3x10 5 3.5x10 2 t3.6x1O 2 1 h 1.lx10 6 i6.Ox10 5 7.9x10 2 ±3.9x10 2 24 h 8.6x10 5 ±2.7x10s 5.7x10 2 *2.5x102 [001341 No significant differences were observed between the NT, 24h and Ih groups in the amount and distribution of granulation tissue. [001351 Time of Application ofpGlcNAc Patch Modulated Foreign Body Response. To study the effects of an extended exposure of the wound bed to the insoluble fibers, the patch was initially left in place for the entire follow-up period (three weeks). The prolonged presence of the insoluble long fibers of the patch induced the formation of a foreign body response, characterized by increased granulation tissue formation and giant multinucleated cells. The application of the patch for I or 24 hours did not induce any foreign body reaction. 6.2 Example 2. Effects of short poly-N-acetlAglucosamine (sNAG) membrane on wound healing in db/db mouse. 6.2.1 Materials and Methods [00136] Preparation ofsNAG Membrane. The sNAG membrane consists of microalgal derived nanofibers produced as described in Section 6.1 supra., in which the fibers are shortened by irradiation. Briefly, the starting material contained 60 g of pGlcNAc slurry at a concentration of 1 mg/mL. The concentration of the pGlcNAc slurry was confirmed by 28 WO 2008/103345 PCT/US2008/002172 filtering 5 mL into a 0.2 um filter. 15 L of pGlcNAc slurry containing 15 g pGlcNAc was filtered until formation of a wet cake. The wake cake was then transferred into a foil pouch, which is a gamma radiation compatible container, and subjected to 200 kGy gamma radiation. Other irradiation conditions were tested for their effects on pGlcNAc compositions, as reflected in Fig. 4A. [00137] Wound Model and Study Design. The genetically mouse model described in Section 6.1 above was used to test the effect of sNAG membranes on wound healing. [001381 Wound Closure Analysis. Wound closure analysis was conducted substantially as described in Section 6.1. The sum of contracted, reepithelialized, and open wound areas equals 100% of the original wound size (Fig. 5) (see Yannas I. Tissue and Organ Regeneration in adults. New York: Springer; 2001). [00139] Immunohistochemistry. Immunochemistry was conducted substantially as described above in Section 6.1. [00140] Vessel Density Quantification. Vessel density quantification was performed substantially as described above in Section 6.1. [00141] Quantification of Cell Proliferation. Quantification of cell proliferation was performed substantially as described above in Section 6.1. [001421 Collagen Staining. Wounds were stained for collagen content using routine methods. [00143] Statistical Analysis. Statistical analysis of wound closure was performed substantially as described above in Section 6.1. 6.2.2 Results [00144] Statistical Analysis. Statistical analysis of wound closure was performed substantially as described above in Section 6.1. [00145] Effect ofIrradiation on pGlcNAc Membranes. While irradiation reduces the molecular weight of pGlcNAc, irradiation did not disturb the microstructure of the fibers. pGlcNAc was irradiated under different conditions: as a dry, lyophilized material; as a dry membrane; as a concentrated slurry (30:70 weight by volume); and as a dilute slurry (5 mg/ml). A suitable molecular weight reduction (to a molecular weight of 500,000-1,000,000 29 WO 2008/103345 PCT/US2008/002172 daltons) was achieved at an irradiation dose of 1,000 kgy for dry polymer, and 200 kgy for wet polymer (Fig. 4A). [00146] The chemical and physical structure of the fibers was maintained throughout irradiation as verified by infrared (IR) spectrum (Fig. 4B), elemental assay, and scanning electron microscopes (SEMs) analysis. Microscopic observation of irradiated fibers showed a decrease in the particle length (Fig. 4C and 4D). The majority of the fibers are less than about 15 pm in length, with an average length of about 4 um. {00147] sNAG Membrane Altered Wound Healing Kinetics. Macroscopic photographs show that the wounds of sNAG membrane treated mice healed faster than the wounds of untreated control mice (see Fig. 6-10). Treatment with sNAG induced faster wound closure over time compared to non-treatment. The sNAG membrane treated mice showed faster (p<0.05) decrease of raw surface (open portion of the wound), compared to the untreated control mice on day 4, 7, 10, 14, 17, 21 and 25 (Fig. 11). [001481 The sNAG membrane treated mice also reached 50% closure on average in a little over 8 days, which is four days faster (p<0.01) than the untreated control mice (over 12 days) (Fig. 12). [00149] The sNAG membrane treated mice also reached 90% closure on average in less than 15 days, which is eight days faster (p<0.01) than the untreated control mice (approximately 23 days) (Fig. 13). [00150] At day 28, all (100%) wounds closed in the sNAG membrane treated mice, whereas only 85% wounds closed in the untreated control mice. [00151] The sNAG membrane treated mice showed augmented (p<0.05) reepithelialization, compared to the untreated control mice on day 14 and 17 (Fig. 14). [00152] The sNAG membrane treated mice showed decreased (p<0.01) contraction, compared to the untreated control mice on day 4, 7 and 10 (Fig. 15). [00153] sNAG Membrane Increased Blood Vessel Density and Proliferation. One cm2 full-thickness, untreated wounds in db/db mouse reached 50% closure between 8-12 days post operation (Chan et aL. Effect of recombinant platelet-derived growth factor (Regranex) on wound closure in genetically diabetic mice. JBurn Care Res 2006;27(2):202-5). Day 10 was chosen as an intermediate time point for staging wound healing following the treatments. 30 WO 2008/103345 PCT/US2008/002172 [001541 The histology of the wound edge in an untreated control mouse vs. in a sNAG membrane treated mouse is shown in Fig. 16 and 17, respectively. Proliferating cells were stained for Ki-67 (Fig. 18). PECAM- 1 (CD3 1) was selected to stain endothelial cells and quantify the blood vessel density in granulation tissue (Fig. 19). The sNAG membrane treated mice showed significant increases in blood vessel density and cell proliferation, compared to the untreated control mice (Fig. 16, 17). [00155] Reepithelialization and granulation tissue area were measured in microphotographs at 4X magnification (n=7-8 per group) to assess the level of coverage by the newly formed tissue in response to injury and treatment modality, as shown in Fig. 20 and 21. [00156] Time of Application ofsNAG Membrane Modulated Foreign Body Response. To study the effects of an extended exposure of the wound bed to the sNAG fibers, sNAG the membrane was left in place during the entire experiment (four weeks). Fig. 22 shows the lack of foreign body reaction at 10 days. Moreover, no foreign body reaction was observed throughout the study in any of the sNAG membrane treated mice. [00157] sNAG Membrane Modulated Collagen Formation/Arrangement. Collagen staining was observed in both sNAG-treated and untreated mice, but collagen bundles were denser and more uniform in the treated mice, suggesting increased stimulation of wound fibroblasts and more advanced wound healing in the treated mice (Fig. 22). 6.3 Example 3. Effects of poly-N-acetylglucosamine (pGlcNAc) and sNAG on Endothelial Cell (EC) Movement and Angiogenesis 6.3.1 Materials and Methods [001581 Tissue Culture, Growth Factors and Transfection. Pooled, multiple-donor human umbilical cord vein endothelial cells (EC) (Cambrex) were maintained at 37*C with 5% CO 2 in endothelial basal medium 2 (Cambrex) supplemented with EC growth medium 2 SingleQuots as described by Cambrex procedures. Serum starvation was performed at 80 90% confluency in RPMI-1640 supplemented with 0.1% fetal calf serum (Gibco BRL) for 24 h followed by stimulation with VEGF 165 (20 ng/ml, R&D Systems) or with highly purified pGlcNAc nanofibers or sNAG nanofibers in sterile water (provided by Marine Polymer Technologies, Inc., Danvers, Mass., USA) with the amounts indicated in the text. For inhibition using the VEGFR inhibitor SU5416 (10 M; R&D Systems) cells were pretreated for 15 min prior to stimulation with VEGF, pGlcNAc or sNAG. 31 WO 2008/103345 PCT/US2008/002172 [00159] Human umbilical cord vein EC were transfected using the Amaxa nucleofector system in procedures described by the manufacturer, obtaining transfection efficiencies up to 80%. All transfections were monitored by expression of green fluorescent protein (GFP) using a GFP expression vector (pFP-C 1; Clontech) or a GFP-directed RNA interference (RNAi; Amaxa). Plasmid-based RNAi directed specifically against Ets 1 was purchased from Pandomics, Inc., and the dominant-negative Ets (dn-Ets) construct contains the DNA-binding domain of Ets2 cloned into a pcDNA3 expression vector. [00160] Antibodies and Western Blot Analyses. The antibodies used for Western blot analysis are as follows: anti-p85 subunit of P13K (Upstate Biotechnology), anti phosphospecific VEGFR2 (Cell Signaling), VEGFR2 (Santa Cruz), antiphospho-specific p42/p44 (Promega), and anti-phospho-specific VEGFR2 (BD Biosciences, Inc.), anti-p42/p44 Erkl/2, anti-VEGFR2 and anti-Etsl (Santa Cruz). [001611 Treated cells were washed once with phosphate-buffered saline (PBS) and lysed in 1 x RIPA lysis buffer (50 mM Tris-HCl, pH 7.5, 1% Triton X-100, 150 mM NaCl, 0.1% SDS, 1% sodium deoxycholate, 40 mM NaF), supplemented with complete protease inhibitors without EDTA (Roche) and 200 pM sodium orthovanadate. Protein concentrations were determined by a bicinchoninic acid protein assay (Pierce) resolved by SDS-PAGE and transferred onto Immobilon-P polyvinylidene fluoride membranes (Millipore). Western analyses followed standard procedures. Proteins were visualized using Luminol reagent (Santa Cruz). 1001621 Cell Motility and Proliferation Assays. For "scratch" wound closure assays, EC were grown to confluence on plastic tissue culture dishes and a single 'wound' was created using a pipette tip. Cells were then incubated in serum starvation media supplemented with or without VEGF (20 ng/ml), pGlcNAc, or sNAG at the amounts indicated in the text for 16 18 h. Cells were washed once with PBS, fixed for 10 min in methanol, stained with 0.1% crystal violet for 10 min and rinsed thoroughly with water. Wounding assays were photographed at 10 x magnification using an Olympic light microscope equipped with digital imaging, and distance migrated was measured. [00163] For modified transwell assays, transfected or untransfected EC were plated onto 8 pm pore size invasion chambers precoated with fibronectin or vitronectin at 20 pg/pl (Sigma), 5 x 104 cells per chamber in 500 pl of serum starvation media, and 500 pl of starvation media was added to the well. VEGF (20 ng/ml), pGlcNAc or sNAG was added to 32 WO 2008/103345 PCT/US2008/002172 the upper chamber. Cells were incubated for 12 h at 37 *C in the presence of 5% CO 2 . Cells that did not migrate were removed by wiping the top of each membrane with a cotton swab. The migrated cells were fixed in methanol for 10 min and stained with 0.1 pg/ml ethidium bromide in PBS. Migrated cells were counted using a Leica fluorescence microscope. Each assay was performed in triplicate at least 3 independent times, and at least 6 fields per transwell were counted. [00164] For in vitro angiogenesis assays, EC were plated onto reduced growth factor Matrigel matrix (BD Laboratory) at 1.6 x 10 4 cells/50 pl per well of a 96-well plate in serum starvation media in the presence or absence of VEGF (20 ng/ml), pGlcNAc or sNAG. Cord formation was assessed for up to 8 h after plating. Cells were fixed and photographed when the VEGF-, pGlcNAc- and sNAG-treated cells began to form cords, while the controls retained a single cell layer. Assays were performed in duplicate and repeated 2 independent times. [00165] For cellular proliferation/viability assessment, 2 different assays were used: trypan blue exclusion by direct cell counts using a hemacytometer and an MTT [3-(4,5 dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] assay in procedures described by the manufacturer (Promega). [00166] Antibody Blockade. To block integrin-mediated cell motility and signaling, EC were preincubated for 15 min with blocking antibodies, at concentrations empirically determined (1 gg/ml), directed against aVp 3 or a 5 s (CD49e) purchased from Chemicon International or against the a5 subunit (Santa Cruz) prior to stimulation with pGlcNAc or sNAG. Normal rabbit serum was used as a negative control. For inhibition of cell migration using the aVp 3 antibody, transwells were precoated with vitronectin (Sigma) rather than with fibronectin (20 pg/pl). To block activation of VEGFR, EC were preincubated for 15 min with inhibitor, SU5416 (SU) at concentration of 10 pg/ml. 1001671 Reverse Transcription Polymerase Chain Reaction. For semiquantitative reverse transcription polymerase chain reaction (RT-PCR), cDNA was synthesized from total RNA (2-5 jpg), isolated using RNA-STAT 60 (Tel-Test, Inc.) in procedures described by the manufacturer, with a Superscript First-Strand Synthesis Kit purchased from Gibco BRL using oligo(dT) following the manufacturer's instructions. PCR reactions contained equal amounts of cDNA and 1.25 siM of the appropriate primer pair (Proligo, Inc.). The primer sequences are as follows: Etsl, forward 5'-TTCTCAGAGCCCAGCTTCAT-3', reverse 5' 33 WO 2008/103345 PCT/US2008/002172 AAAGTTTGAATTCCCAGCCAT-3'; metallothionein 2A, forward 5' CAACCTGTCCCGACTCTAGC-3', reverse 5'-AGGAGCAACTCCTGTCCTGA-3'; S26, forward 5'-CTCCGGTCCGTGCCTCCAAG-3', reverse 5' CAGAGAATAGCCTGTCTTCAG-3'; VEGF, forward 5'-CTACCTCCACCATGCCAAGT 3', reverse 5'-TGGTGATGTTGGCTCCTCA-3'; IL-1, forward 5' CTGCGCCAACACAGAAATTA-3', reverse 5'-ATTGCATCTGGCAACCCTAC-3'; IL-8, forward 5'-TCGGATTTCACGATTTCTCC-3', reverse 5'-GCTACAAGTGCGTCGTCAAA 3'. Cycling conditions were: 94*C for 5 min; 20-35 cycles of 94*C for I min, 50-65*C (based on primer T m) for I min, 72 0 C for I min and 45 s + 2 s/cycle; 72*C for 7 min and cooled to 4*C. The cycle number was empirically determined to be within the linear range of the assay for each primer pair used. All semiquantitative RTPCRs were performed in tandem with S26 primers as an internal control. Products were run on 1-1.5% agarose gels (based on product size) and visualized on a BioRad Molecular Imaging System. [001681 Real-time PCR was performed using a Brilliant CYBR green quantitative PCR (QPCR) kit in combination with an Mx3000P real-time PCR system, both purchased from Stratagene. Real time was performed at least in triplicate at least 2 independent times. Internal control primers that detect the ribosomal protein subunit S26 were used. 6.3.2 Results 6.3.2.1 pGlcNAc [00169] pGlcNAc Protected ECfrom Cell Death Induced by Serum Starvation. To test if pGlcNAc fibers had a direct effect on EC, serum-starved EC cells were treated with VEGF or with different concentrations of pGlcNAc fibers. As shown in Fig. 24 at 48 h and 72 h after serum starvation, as compared with the total number of cells plated (control), there was about 2-fold reduction in the number of cells after 48 h or 72 h. At 48 h, this decrease in cell number was rescued by the addition of VEGF or by the addition of pGlcNAc fibers at either 50 or 100 ptg/ml. At 72 h, the decrease in cell number was rescued by the addition of VEGF or largely rescued by the addition of pGlcNAc fibers at 100 ig/ml. These results indicated that like VEGF, pGlcNAc fiber treatment prevented cell death induced by serum deprivation. [00170] pGlcNAc did not effect metabolic rate. As shown in Fig. 25, pGlcNAc did not result in a higher metabolic rate as measured by MTT assays, indicating that this polymeric material was not causing marked increases in cellular proliferation but was rescuing cell death by serum deprivation. 34 WO 2008/103345 PCT/US2008/002172 [00171] pGlcNAc Increased Cell Migration. To test whether pGlcNAc fiber treatment of EC resulted in changes in cell motility, the "scratch" wound closure assay was used. Migration of the cells into the wounded area was significantly increased in the presence of pGlcNAc at both 50, 100 and 250 gg/ml. As shown in Fig. 26, wound closure was similar to that observed for VEGF treatment. These results indicated that pGlcNAc treatment resulted in an increase in EC movement. 1001721 pGlcNAc Caused an Increase in Migration Towards Fibronectin. To determine whether this increased cell motility correlated with an increased cellular invasion, EC motility was measured using transwell assays where membranes were precoated with the extracellular matrix protein, fibronectin. As shown in Fig. 27, pGlcNAc treatment resulted in a 3-fold increase in migration toward fibronectin that was enhanced by the addition of VEGF (4-fold). [00173] pGlcNAc Increased Cord Formation. Stimulation of cell migration is a prerequisite for increased angiogenesis. To test, in vitro, whether pGlcNAc was proangiogenic, Matrigel assays were performed. EC were plated on growth factor-reduced Matrigel under conditions of serum starvation and assessed for cord formation in the presence or absence of VEGF or pGlcNAc fibers within 6 h. As shown in Fig. 28, both VEGF and pGlcNAc treatment resulted in increased cord formation on Matrigel. These results indicate that pGlcNAc is proangiogenic. [00174] pGlcNAc Induced Effectors Involved in Cell Motility. Total RNA was isolated from serum-starved EC (SS); SS treated with VEGF, pGlcNAc, Sphingosiing 1-phosphate (SlP), or Zn; SS pre-treated with VEGF or SIP then following pGlcNAc treatment; SS pre treated with VEGF or pGlcNAc then following the treatment of VEGFR inhibitor, SU5416 (Su). As shown in Fig. 29A, pGlcNAc treatment stimulated the expression of the Etsl transcription factor, which is an important regulator of EC movement, and metallothionein 2A (MT), Akt3 and Edg3. As shown in Fig. 29B, real-time PCR indicates that Etsl was induced approximately 2-fold by pGicNAc treatment. This Etsl increase in message was accompanied by a higher protein expression as shown in the Western blot analysis in Fig. 29C. Fig. 29A also indicated that MT expression stimulated by pGlcNAc was VEGFR dependent. [00175] pGlcNAc's Induction of Phospho-MAPK was VEGFR2-Dependent. Totest whether pGlcNAc treatment resulted in the activation of pathways previously shown 35 WO 2008/103345 PCT/US2008/002172 downstream of VEGFR signaling, EC were treated with VEGF or pGlcNAc. As shown in Fig. 30A, pGlcNAc treatment resulted in a marked increase in the phosphorylation of MAPK. To test whether this increase was VEGFR2 dependent, EC were pretreated by VEGFR inhibitor, following VEGF or pGlcNAc treatment. The results indicated that pGlcNAc's induction of phosphor-MAPK was dependent on VEGFR2 (see also Fig. 30B). [00176] pGlcNAc did not activate VEGFR2. To test whether pGlcNAc activated the VEGFR, a series of Western blots were performed using an antibody directed against the phosphorylated form of VEGFR2. As shown in Fig. 31, VEGF treatment caused a rapid phosphorylation of VEGFR, accompanied by turnover of total VEGFR2 protein levels, whereas pGlcNAc had no effect either at these early time points shown, or up to 6 h after treatment (data not shown). [00177] pGlcNAc-Induced Migration was Ets Dependent. To test whether Etsl was required for the motility induced by pGlcNAc, Ets1 was inhibited using both a dominant negative approach as well as by RNAi. A dn-Ets construct expressing the conserved Ets DNA binding domain was transfected into EC. After 24 h to allow for expression of the dn-Ets, the cells were assessed for changes in cell migration toward fibronectin in transwell assays after treatment with pGlcNAc. As shown in Fig. 32A, inhibition of Etsl activity as well as of other family members expressed in EC resulted in a marked decrease in EC migration in response to pGlcNAc. As a control for the activity of the dn-Ets, Fig. 5B shows that transfection of increasing amounts of dn-Ets results in a decreased total Ets 1 protein. Ets I expression can be controlled not only by another family member, but can also be autoregulated. Inhibition of Etsl specifically by RNAi also resulted in a decrease in cell motility induced by pGlcNAc on fibronectin (Fig. 32A, right side). As shown in Fig. 32B, in cells transfected with dn-Ets expression plasmid, the Etsl protein expression decreased with the amount of plasmid increasing. As a control for the RNAi experiment, Fig. 32C shows the resultant expression levels of Etsl in EC transfected with 2 amounts of plasmid-containing RNAi directed against Etsl. Expression of dn-Ets resulted in a more substantial reduction in cell migration than the Etsl RNAi, probably due to its blockade of other family members expressed in EC. This findings supported a role for Etsl in the induction of cell motility by pGlcNAc. [00178] pGlcNAc-Induced Cell Motility Required Integrin. To test whether the effects of pGlcNAc are integrin dependent, blocking antibodies were used to disrupt integrin-mediated signaling in EC. The effect of these antibodies on pGlcNAc-induced cell migration was assessed using transwell assays. Fig. 33A shows the results when using antibodies directed 36 WO 2008/103345 PCT/US2008/002172 against aVp 3 or a 5 pI (CD49e) integrin in migration assays toward fibronectin (the aVp3 receptor). Fig. 33B shows a similar experiment using antibodies directed against aVp3 or asp I (CD49e) in transwells coated with vitronectin. Antibody blockade of either integrin subtype resulted in inhibition of pGlcNAc-induced cell motility on their cognate substrates. These results indicate that pGlcNAc stimulates cell motility via integrin activation. The results are also consistent with pGlcNAc stimulating angiogenesis via integrin activation. [00179] pGlcNAc-Induced Cell Motility May Involve the Activation of FAK via Integrin Engagement. FAK becomes phosphorylated in response to integrin clustering and activation. FAK is a key regulator of integrin and growth factor-mediated cell motility and invasion. To test integrin activation by pGlcNAc, EC were treated with pGlcNAc fibers for increasing amounts of time and assayed for changes in the level of FAK phosphorylation. As shown in Fig. 34, pGlcNAc treatment resulted in the phosphorylation of FAK within 15 min of treatment. These results indicate that pGlcNAc-induced cell motility may involve the activation of FAK via integrin engagement. [00180] pGlcNAc Activates an Integrin--+Ets1 Pathway Leading to Angiogenesis in a Wound-Healing Model. A role for Etsl in the transcriptional regulation of a number of integrin subunits has been described, positioning Etsl upstream of integrins. The finding that motility induced by pGlcNAc is dependent both on integrins and Etsl implies that Etsl may be regulated downstream of integrins. To confirm that integrin activation results in the regulation of Ets 1 expression, blocking antibodies directed against asp I (the fibronectin receptor) or aVp3 (the vitronectin receptor) were used to inhibit integrins by pGlcNAc. Fig. 35A shows that antibody blockade of aspi integrin results in a reduction in pGlcNAc-induced Etsl expression. This inhibition of Etsl expression using a blockade of ass, integrin is recapitulated on the protein level (Fig. 35B). However, although the aVp 3 integrin antibody blocked motility on vitronectin, it did not affect the pGlcNAc-induced expression of Ets 1 (Fig. 35), indicating that pGlcNAc-induced cell motility on vitronectin may be Etsl independent. Taken together, these results position Ets 1 downstream of certain integrins in primary EC and indicate potential specificity in integrin signaling with respect to Etsl expression in primary EC. These findings, therefore indicate that pGlcNAc can activate an integrin--+Etsl pathway leading to angiogenesis in a wound-healing model. [00181] pGlcNAc Induced Expression of VEGF and ILL. To test whether pGlcNAc treatment induced the expression of growth factors or cytokines known to be secreted by activated EC, serum-starved EC were treated with pGlcNAc for 12 h and assessed for 37 WO 2008/103345 PCT/US2008/002172 changes in expression of VEGF, IL-I and IL-8. As shown by RT-PCR and QPCR (Fig. 36A), pGlcNAc treatment resulted in an increased expression of both VEGF and IL-1. These findings also indicated that the response of the EC to pGlcNAc is specific since there was no change in expression of another interleukin, IL-8. To test the pGlcNAc-dependent induction of Etsl expression, a transcription factor known to be regulated by VEGF, secondary to the pGlcNAc effect on VEGF expression, activation of VEGFR was blocked using the pharmacological inhibitor, SU5416 (SU), prior to treatment with pGlcNAc. As shown by QPCR (Fig. 36B), treatment of EC with this inhibitor blocked the induction of Etsl by VEGF but had no effect on the induction of Etsl by pGlcNAc. [00182] pGlcNAc Induced Expression of FGF1 and FGFR3. To test whether pGlcNAc treatment induced the expression of angiogenesis-related factors, EC were treated with pGlcNAc and assessed for changes in expression of FGF1, FGF2, FGFR1, FGFR2, FGFR3, Stabilin, IFNg, CollagenA18. As shown by Fig. 37, pGlcNAc treatment resulted in an increased expression of Stabilin and CollagenA 18. 6.3.2.2 sNAG [001831 sNAG Increased Cell Migration. To test whether sNAG fiber treatment of EC resulted in changes in cell motility, the "scratch" wound closure assay was used. Migration of the cells into the wounded area was significantly increased in the presence of sNAG at both 50 and 100 pg/ml. Wound closure was similar to that observed for pGlcNAc treatment (see Fig. 38). These results indicated that sNAG treatment resulted in an increase in EC movement. [00184] sNAG Induced Marked Increase in Metabolic Rate. As measured by MTT assays, sNAG at 50, 100 or 200 pg/ml resulted in a higher metabolic rate of EC than VEGF (Fig. 39). [00185] sNAG did not protect ECfrom cell death induced by serum deprivation. To test if sNAG fibers had a direct effect on EC, serum-starved EC cells were treated with VEGF or with different concentrations of sNAG fibers. As shown in Fig. 40, at 48 h after serum starvation, as compared with the total number of cells plated (control), there was about 2-fold reduction in the number of cells. This decrease in cell number was rescued by the addition of VEGF but not by the addition of sNAG fibers at 50, 100 or 200 pg/mil. These results indicated that not like VEGF, sNAG fiber treatment did not prevent cell death induced by serum deprivation. 38 WO 2008/103345 PCT/US2008/002172 [001861 sNAG Induced Expression of VEGF and IL1. To test whether sNAG treatment induced the expression of growth factors or cytokines known to be secreted by activated EC and compare the effect with pGlcNAc, serum-starved EC were treated with pGlcNAc or sNAG for 12 h and assessed for changes in expression of VEGF, IL-1 and IL-8. As shown by Fig. 41, sNAG treatment resulted in an increased expression of both VEGF and IL-1. These findings also indicated that the response of the EC to sNAG is specific since there was no change in expression of another interleukin, IL-8. [00187] sNAG Induced Expression of FGFJ and FGFR3. To test whether sNAG treatment induced the expression of angiogenesis-related factors, EC were treated with sNAG and assessed for changes in expression of FGFI, FGF2, FGFR1, FGFR2, FGFR3, Stabilin, IFNg, CollagenA 18. As shown by RT-PCR (Fig. 37), sNAG treatment resulted in an increased expression of FGF1 and FGFR3. [00188] The above results demonstrate that both pGlcNAc and sNAG induced EC motility, and that both pGlcNAc and sNAG induce the expression of VEGF and IL-1. [00189] The above results also demonstrate that sNAG increases the metabolic rate of serum-starved EC in a MTT assay and does not rescue apoptosis of serum-starved EC in a trypan blue exclusion test. 6.4 Example 4. Preclinical Testing of sNAG 6.4.1 Test Article [00190] A test article comprising sNAG produced as previously described in Section 6.2.1 supra. was utilized. The test article was supplied sterile by Marine Polymer Technologies, Inc. 6.4.2 Biocompatibility Testing - L929 MEM Elusion Test - ISO 10993-5 [00191] Biocompatibility of the test article was tested in mouse fibroblast L929 mammalian cells. No biological reactivity (Grade 0) was observed in the L929 cells at 48 hours, post exposure to the test article. The observed cellular response obtained from the positive control article (Grade 4) and negative control article (Grade 0) confirmed the suitability of the test system. Based on the criteria of the protocol, the test article is considered non-toxic and meets the requirements of the Elution Test, International Organization for Standardization (ISO) 10993-5 guidelines. See Table I below. 39 WO 2008/103345 PCT/US2008/002172 Table I ___________REACTifTY GR ADIS T est A rticle R E Coutrok Medium Negative Positir A B C A B C A B C A B C Flows 0 0 0 0 0 0 G 0 0 0 0 0 24Hom 0 0 0 0 0 0 0 0 0 3 3 3 48Hos 0 0 0 0 0 0 0 0 0 44 Gnade Reactiity Description of Resctivity Zone 0 None Discrete incytoplasmic ganules; no cell lysis Not more the 20% ofthe ceas ar roud. loosely amched.and without finncytoplamic gmnules; occasionally-ed celLs are preset S.NNot more than 50%of the cells are roimd and devoid ofiutracycoplasmic grmule no extensive cell lysis aud empty reas between cells 3 Moderste Not more than 70% of the cell Layer courin rounded cels or are lysed 4 Severe Nearly complete desmuttion of the cell layers 6.4.3 Intramuscular Implantation Test - ISO - 4 Week Implantation 6.4.2.1 Materials and Methods [001921 To evaluate the potential of the test article to induce local toxic effects, the Intramuscular Implantation Test - ISO - 4 Week Implantation ("Intramuscular Implantation Test") was used. Briefly, the test article was implanted in the paravertebral muscle tissue of New Zealand White rabbits for a period of 4 weeks. The test article was then evaluated separately using two control articles: positive control Surgicel (Johnson and Johnson, NJ) and negative control High Density Polyethylene (Negative Control Plastic). [00193] Preparation of Test and Control Articles. The test article measured approximately 1 mm to in width and 10 mm in length. The two control articles were prepared. The positive control, Surgicel (C1), measured approximately 1 mm in width by 10 mm in length and was received sterile. Negative Control Plastic (C2), measured approximately 1 mm in width by 10 mm in length and was sterilized by dipping in 70% ethanol. [00194] Pre-Dose Procedure. Each animal was weighed prior to implantation. On the day of the test, the dorsal sides of the animals were clipped free of fur and loose hair was removed by means of a vacuum. Each animal was appropriately anesthetized. Prior to implantation, the area was swabbed with a surgical preparation solution. 40 WO 2008/103345 PCT/US2008/002172 [001951 Dose Administration. Four test article strips were surgically implanted into each of the paravertebral muscles of each rabbit, approximately 2.5 cm from the midline and parallel to the spinal column and approximately 2.5 cm from each other. The test article strips were implanted on one side of the spine. In a similar fashion, positive control article strips (Surgicel) were implanted in the contralateral muscle of each animal. Two negative control strips (Negative Control Plastic) were implanted caudal (toward the tail) to the test article and to Cl control implant sites on either side of the spine (total of four strips). A total of at least eight test article strips and eight of each control article strips are required for evaluation. [001961 Post-Dose Procedures. The animals were maintained for a period of 4 weeks. The animals were observed daily for this period to ensure proper healing of the implant sites and for clinical signs of toxicity. Observations included all clinical manifestations. At the end of the observation period, the animals were weighed. Each animal was sacrificed by an injectable barbiturate. Sufficient time was allowed to elapse for the tissue to be cut without bleeding. [001971 Gross Observations. The paravertebral muscles in which the test or control articles were implanted were excised in toto from each animal. The muscle tissue was removed by carefully slicing around the implant sites with a scalpel and lifting out the tissue. The excised implant tissues were examined grossly, but without using excessive invasive procedures that might have disrupted the integrity of this tissue for histopathological evaluation. The tissues were placed in properly labeled containers containing 10% neutral buffered formalin. [00198] Histopathology. Following fixation in formalin, each of the implant sites was excised from the larger mass of tissue. The implant site, containing the implanted material, was examined macroscopically. Each site was examined for signs of inflammation, encapsulation, hemorrhaging, necrosis, and discoloration using the following scale: 0 = Normal 1 = Mild 2 = Moderate 3 = Marked After macroscopic observation, the implant material was left in-situ and a slice of tissue containing the implant site was processed. Histologic slides of hematoxylin and eosin stained 41 WO 2008/103345 PCT/US2008/002172 sections were prepared by Toxikon. The slides were evaluated and graded by light microscopic examination. [001991 Pathological Assessment ofthe Effects of the Implant. The following categories of biological reaction were assessed by microscopic observation for each implant site: 1. Inflammatory Responses: a. Polymorphonuclear leukocytes b. Lymphocytes c. Eosinophils d. Plasma cells e. Macrophages f. Giant cells g. Necrosis h. Degeneration 2. Healing Responses: a. Fibrosis b. Fatty Infiltrate [002001 Each category of response was graded using the following scale: 0 = Normal 0.5 = Very Slight 1 = Mild 2 = Moderate 3 = Marked 1002011 The relative size of the involved area was scored by assessing the width of the area from the implant/tissue interface to unaffected areas which have the characteristics of normal tissue and normal vascularity. Relative size of the involved area was scored using the following scale: 0 = 0 mm, No site 0.5 = up to 0.5 mm, Very slight 1 = 0.6 - 1.0 mm, Mild 2= 1.1 - 2.0 mm, Moderate 3 => 2.0 mm, Marked [002021 The Intramuscular Implantation Test was conducted based upon the following references: 42 WO 2008/103345 PCT/US2008/002172 1. ISO 10993-6, 1994, Biological Evaluation of Medical Devices - Part 6: Tests for Local Effects After Implantation. 2. ISO 10993-12, 2002, Biological Evaluation of Medical Devices - Part 12: Sample Preparation and Reference Materials. 3. ASTM F981-04, 2004, Standard Practice for Assessment of Compatibility of Biomaterials for Surgical Implants with Respect to Effect of Materials on Muscle and Bone. 4. ASTM F763-04, 2004, Standard Practice for Short Term Screening of Implant Materials. 5. ISO/IEC 17025, 2005, General Requirements for the Competence of Testing and Calibration Laboratories. [002031 The results of the Intramuscular Implantation Test were evaluated based upon the following criteria: 1. Calculated Rating: For each implanted site, a total score is determined. The average score of the test sites for each animal is compared to the average score of the control sites for that animal. The average difference between test and control sites for all animals is calculated and the initial Bioreactivity Rating is assigned as follows: 0 - 1.5 No Reaction* > 1.5 - 3.5 Mild Reaction > 3.5 - 6.0 Moderate Reaction > 6.0 Marked Reaction * A negative calculation is reported as zero (0). 2. Modification of the Rating: The pathology observer reviews the calculated level of bioreactivity. Based on the observation of all factors (e.g., relative size, pattern of response, inflammatory vs. resolution), the pathology observer may revise the Bioreactivity Rating. Justification for the modification to the rating is presented in the narrative report (A descriptive narrative report regarding the biocompatibility of the test material is provided by the pathology observer). 6.4.2.2 Results [00204] The results indicated that the test article was non-reactive when implanted for 4 weeks (Bioreactivity Rating of 0.2) when compared to positive control Surgicel; and non reactive (Bioreactivity Rating of 0.0) when compared to negative control High Density Polyethylene (Negative Control Plastic). 43 WO 2008/103345 PCT/US2008/002172 [002051 Clinical observation. Table II below shows results of the macroscopic evaluation of the test article and control implant sites indicated no significant signs of inflammation, encapsulation, hemorrhage, necrosis, or discoloration at the 4 week time period. Some test sites and the majority of the positive control, Surgicel, were not seen macroscopically and TABLE H Macroscopic Observations 4 Week Implantation Animal No.: 60959 Tissue Site: TI T2 TJ T4 TetC.1 Ccno Control A.I I c-1- cl- CI.4 'JIN- CZ C2.2 CQ-3 C2-i i4 , Inflammatdon 0 NWF 0 NSF 0 NSF NSF NSF NSF NIA 0 0 0 0 0 Encapsulatio 0 NSF 0 NSF 0 NSF NSF NSF NSF N/A 0 0 0 0 0 Henorrbhsg 0 NSF 0 NSF 0 NSF NSF NSF NSF N/A 0 0 0 0 0 Necrosis 0 NSF 0 NSF 0 NSF NSF NSF NSF N/A 0 0 0 0 0 Discoloralien 0 NSF 0 NSF 0 NSF NSF NSF NSF N/A 0 0 0 0 0 Tota 0 N/A 0 N-A N/A NfA N/A N/A 0 0 0 0 Animal No.: 60961 Tissue Site: TI Ti T3 T4 Tes. Cl-1 Cl-2 C14- Cl-4 coA" C2-1 C - 4 C- C2-4 Conro. Inhammation NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 NSF 0 0 Encapsulaton NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 NSF 0 0 Hemorrhare NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 NSF 0 0 Necrosis NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 NSF 0 0 Discoloraion NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 NSF 0 0 TOWa I N/A N/A N/A N/A N/A N/A N/A N/A 0 0 N/A 0 Animal No.: 60968 Tissue Site: TI Ti T3 T4 Tet Cl-I Cl-2 C1- C14 C 2l 1-1 C2-2 C2-4 C24 Conro. lnf.i- On NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 0 0 0 EcpsuLation NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 0 0 0 Hemorage NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 0 0 0 Ncrosis NSF NSF NSF NSF N/A NSF NSF NSF NSF N/A 0 0 0 0 0 Discoloradon NSF NSF NSF NSF V'A NS NSF NSF N/A 0 0 0 0 0 Tom NIA N/A N/A N/A N/A N/A N/A N/A 0 0 0 0 T= test site (representative sections were submitted for microscopic assessment) C1 = Surgicel (Due to the nature of the material, representative sections were submitted for microscopic assessment) C2 - Negative Control High Density Polyethylene (Negative Control Plastic) Grading Scale 0 - no reaction 2 - moderate reaction NSF = No Site Found 1 - mild reaction 3 - marked reaction N/A - Not Applicable serial sections were submitted for microscopic evaluation. [002061 Implantation Site Observations (Microscopic). Table III below shows results of the microscopic evaluation of the test article implant sites indicated no significant signs of inflammation, fibrosis, hemorrhage, necrosis, or degeneration as compared to each of the 44 WO 2008/103345 PCT/US2008/002172 control article sites. The Bioreactivity Rating for the 4 week time period (average of three animals) was 0.2, (Cl - Surgicel) and 0.0 (C2 - Negative Control Plastic) indicating no reaction as compared to either of the control implant sites. The pathologist noted there was a moderate polymorphic and histiocytic (macrophages) infiltrate around the in situ test article that was not unexpected given the nature of the test material. TABLE III Microscopic Observations 4 Week Implantation Animal No.: 60959 Categories Test Sites* Control Sites Reaction T1 T. T3 Cl-i C1-2 Cl-3 C1-4 C2-1 C2-2 C2-3 C2-4 Oreigp Debis 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 eize of Involved a 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 * Po0.0 0.500.0 0.5 0 0.0 0 .0 0 .0 0.0 0.0 0.0 L)aphocytes 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Eosinophils 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * Plasma Cells 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * Macrophages 0.5 0.5 0.5 0.5 0.5 0.5 05 0.5 0.5 0.5 0.5 Giant Cells 0.0. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Degenermion 0.5 0.5 0.5 0.5 0.0 0.5 0.0 0.0 0.0 0.0 0.0 * Necrosis 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Fibrosis 0.5 0 . 0.5 0 . 00.5 0.5 0.5 0.5 0.5 FattyInflilate 0.0 0.0 0.5 0.0 0.5 0.0 05 0.5 0.5 0.0 0.5 Total 1.5 2.0 2.5 1. 1.5 1. 1.5 [ 1.5 1.0 11.5 T - Test Site Cl -Surgicel C2 = Negative Control High Density PolyetIylene (Negative ControlPlastic) Animal Test Score (Aveaage*) 2.0 Animal C1 Score (Aveage*)= 1.5 Animal C2 Score (Average*) - 1.4 Animal Score (Average Test Score - Average Cl Score) 0.5 Animal Score (Average Test Score - Average C2 Score) = 0.6 * Used in calculation of Bioreactivity Rating. ** No site foundin T4. 45 WO 2008/103345 PCT/US2008/002172 TABLE I Microscopic Observations (Cont.) 4 Week Implantation AnimalNo,: 60961 Categoies Test Sites** ContolSites** Reaction T1 '3 T4 Cl-I Cl-3 CI C-1 C2-2 C2-3 Foreign Debuis 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Re. Size of M d area 03 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 -PolyNmophs 0.0 0.0 0.5 0.5 0.0 0.5 0.5 0.5 0.5 u Lymphcytes 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * Eosiuophs 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Phsma Ces 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * auoviges 03 0.5 0.5 05 0.5 0.5 0.5 0.5 0.5 * GiantCells 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * Degpmam0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Neaosis 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * Fibrosis 03 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 v Fatty Infihrte 0.0 0.5 0.0 0.5 0.0 05 0.5 0.5 0.5 Total 1.5 2.0 2.0 2.5 1 2. - 2,5 2.5 M. T=Test Site C1 =Surical C2 = Negative Cowol Hig4Desity Polyedylwe Qn(egatve ConolPlastIc) Animal TestScore (Aveae*) 1.8 Animal C1 Scoie (Aveage*) = 2.2 Aninm1 C2 Score (Average*) - 2.5 Animal Score (Average Test Score-Average CI Score)= -0.4 Animal Score (Average Test Score. Average C2 Score) = -0.7 * Used in calcularen ofBioreactivity Rating. SNosite fondin T2, C1-2, and C2-4. 46 WO 2008/103345 PCT/US2008/002172 TABLE IH Microscopic Observations (Cont.) 4 Week Implantation Animal No.: 6096S Categories Test Sites Control Sites" Reaction T1 12 T3 T4 Cl-1 Cl-2 C1-3 C2-1 C2-2 C2-3 C2-4 Foreign Debris 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Rel.SizeofInvolvedarea 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 * Polymarphs 0.0 0.5 0.0 0.5 0. 0.0 0.0 0. 0.5 0.0 0.5 *Lyrphocytes 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.00 0.0 0.0 * Eosinophils 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * Plasma Cells 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * Maavphaes 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 * Giat Cells 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 * Degenetion 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 *Nesis 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 *Fbirosis 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 * Fanynfiltate 0.5 0.5 0.5 0.5 0.5 0.0 0.5 0.5 0.5 0.5 0.5 Total 2.0 2.5 2.0 2.5 2.0 1.5 2.0 2.5 2.5 2.0 2.5 T = Test Site C1 = Sugicel C2 - Negative Control High Density Polyethylene (Negative Control Plastic) Animnl Test Score (Average*)= 2.3 Animl C1 Score (Average*) = 1.8 Animal C2 Score (Avernge*)= 2.4 Animal Score (Average Test Score - Average C1 Score) = 0.5 Animal Score (Average Test Score - Average C2 Score) = -0.1 * Used in calculation ofBioreactivity Rating. ** No site found in C1-4. C1 C2 Animal Score 60759 = 0.5 0.6 Animn1 Score60961= -0.4 -0.7 Animn1Score60968= 0.5 -0.1 Bioreactiity Rating - 0.2 =No Reaction Bioreactihity Rating = -0.1 = No Reaction 6.4.4 Intracutaneous Injection Test - ISO 10993-10 100207] USP 0.9% Sodium Chloride for Injection (NaCI) and Cottonseed Oil (CSO) extracts of the test article were evaluated for their potential to produce irritation after intracutaneous injection in New Zealand White rabbits. The test article sites did not show a significantly greater biological reaction than the sites injected with the control article. Based 47 WO 2008/103345 PCTIUS2008/002172 on the criteria of the protocol, the test article is considered a negligible irritant and meets the requirements of the ISO 10993-10 guidelines. Results are shown below in Table IV. Table IV lIfltcutaneous Test Skia~ Rtactiou Scores Nsti Eitrsec ISit Numbers *Animal#~ Vehicle Time, Scrivi MR/E - T-1 T-2 T-3 T-4 r3 C-1 C-2 C-3 C-d C-S 0hus . WD 010 0/0 W 0 0 .0A 010 01 0 0NO 011 61917 Nac 24 h"wr% D/0 vv:1 WO) Oe o0d) 0:0 0 0110 00 0/I 48 hottrs 0;0 0.10 010 NO0 M/ 0!0 Olt 010 0/0 6)v - 72,hours 0:0 0:0 (IA 0/0 &.0 0:0 OM0 61 0 OM W 0 hottT 0110 010 0/001!' 001 fo NO 00 10 010 0oI10. 61019 N40 24 kV. Of!0 0.1) 010 010 &/0 :0 NO0 011) 0/0 010 48 houart 0:0 W) O'D Q.-o 0o.o 0-0 Ov 0' 0'.0 0.4) 72 hours 0;0 00 010 0/0 (k0 0-0 0(0 G/G (LID Wo TO0W 0.0 *Ilniledistely kifer io~ijiim. tbot used fOv 71 te vuluitiun critiria. Ovrrafl Mctan Scam v fr Test. Mick - 0.0 OqcrdallMear; Score$ lot Control Arikle 0.10 Difiemec berwenen c Ankck and Control Ankle Overoll Mean Scav - 0.0-0.0 0.0 Anial N Vhicle That ___ - -Sanim(ERIEDI 1-i T-2 iT-3 T-4 T-S C-1 C-2 C-3 C-__ .

0 how" 0&0 410 0O0 0"0 0.10 00 090 (o~ G/ 010 61917 ~ CS 24 hour-. tPO0 0 /0 O 0;C 0 01 ;0 0:0 'ID 010 00 48 hours 0,'D 0,0 0'V 0.9 0 0 '0 0 !0 0 -1) (YO00 / - - 72 haws~ 0*0 010 0.10 0.10 01VOM0/ 010 0.1-10 o 0--0 a hWUT$*t 01) 00) 010O Oro 010 010) 010 0/0 0/0 0/0 61919 CS05 2 hours 11-V AV 0 01 10. 0 r0 01V 0/0 010 0 10 OA (tt -18 hours 0M 00 M/ 0.10 0'0 0.10 040 010 (110 010 7_____ 01G10 ,OsO IA WO 1 I 1 0.'0 00 /0 0/ Total 0.0 10 ' 0 0.0 Ot t 1ne c-iLna.I ar injcwtk. tiot used fiar the evalusallon criteria. OrMIINean %cowe for -rest Article 0.0 0%rall Mngaz Score* Ubr Control Artick - I) ( Dilaeren" bewtvn res Aticbe and Coma]o Artile Chverall Mean Score =0.0-0.0 -0.0 ER Evyibczn, ED-Edcina: T r=s Steuas: C zrControl Sites * CcraII Mcan Score= 1-twil crytlama pins edtri SCoria divided by 12 Q anirraI% - scoringg periods -2 ecoritg caweois) 6.4.5 Kliaman Maximization Test - ISO 10993-10 [00208] UPS 0.9% Sodium Chloride for Injection (NaCi) and Cottonseed Oil (CSO) extracts of the test article elicited no intradermal reaction in Hartley guinea pigs at the challenge (0% sensitization), following an induction phase. Therefore, as defined by the 48 WO 2008/103345 PCT/US2008/002172 scoring system of Kligman, this is a Grade I reaction and the test article is classified as having weak allergenic potential. Based on the criteria of the protocol, a Grade I sensitization rate is not considered significant and the test article meets the requirements of the ISO 10993 10 guidelines. Results are shown below in Table V. Table V Skin Examination Data Scores percent Akel Group Animal # Se Dav 25 Dav 26 Dav 27 Aulmals Poteal I Male 0 0 0 2 Mak 0 0 0 3 Male 0 0 0 4 Male 0 0 0 Test Attkle 5 Male 0 0 0 weak (NaCI Extract) 6 Female 0 0 0 7 Female 0 0 0 8 Female 0 0 0 9 Female 0 0 0 10 Female 0 0 0 It Male 0 0 0 12 Male 0 0 0 13 Male 0 0 0 14 Male 0 0 o' Test Article Is Male 0 0 0 (CSO Extract) 16 Female 0 0 0 17 Female 0 0 0 Is Female 0 0 0 19 Femalo 0 0 0 20 Female 0 0 0 21 Male 0 0 0 22 Male 0 0 0 c 23 Femaie 0 0 0 0% wek 24 Female 0 0 0 25 Female 0 0 0 26 Male 0 0 0 Negnve 27 'Male 0 0 0 Contro 0) 28 Female 0 0 0 0% weak 29 Female 0 0 0 30 Femalo 0 0 0 31 Male 2 1 0 Positive Control 32 Male 2 2 1 (DC) 33 Fenmbla 3 2 1 100% exinmue 34 Femaic 3 2 1 35 Femald 3 3 2 Sensitiaation Rate (%) Grade Class 0-8 1 Weak 9-28 1 Mild 29-64 Moderate 65-80 IV Strong 81-100 VExtreme The test results are interpreted based upon the pcrcentage sensitization observed. 49 WO 2008/103345 PCT/US2008/002172 7. SPECIFIC EMBODIMENTS, CITATION OF REFERENCES [00209] The present invention is not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description and accompanying figures. Such modifications are intended to fall within the scope of the appended claims. [002101 Various references, including patent applications, patents, and scientific publications, are cited herein; the disclosure of each such reference is hereby incorporated herein by reference in its entirety. 50

Claims (35)

1. A method for treating a chronic wound in a human subject, comprising: (a) topically applying a composition comprising biocompatible poly--1--4 N-acetylglucosamine fibers to a chronic wound in a human subject in need thereof, wherein the poly-3-1-4-N-acetylglucosamine comprises at least 70% of N acetylglucosamine monosaccharides, and (b) repeating said application every 5 to 18 days, wherein the application is repeated at least twice.

2. Use of a composition comprising biocompatible poly--1--4-N acetylglucosamine fibers, wherein the poly--1--4-N-acetylglucosamine comprises at least 70% of N-acetylglucosamine monosaccharides, in the manufacture of a medicament for treating a chronic wound in a human subject comprising: (a) topically administering the composition to a chronic wound in a human subject in need thereof, and (b) repeating said application every 5 to 18 days, wherein the application is repeated at least twice.

3. The method according to claim 1 or use according to claim 2, wherein step (b) comprises repeating said application every 5 to 12 days.

4. The method according to claim 1 or use according to claim 2, wherein step (b) comprises repeating said application every 7 to 14 days.

5. The method or use according to any one of claims 1-4, wherein the chronic wound is a cutaneous ulcer.

6. The method or use according to claim 5, wherein the cutaneous ulcer is a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, or a pressure ulcer.

7. The method or use according to claim 6, wherein the cutaneous ulcer is a venous stasis ulcer.

8. The method or use according to any one of claims 1-4, wherein the chronic wound is a surgical wound or a burn wound.

9. The method or use according to any one of claims 1-8, wherein the human subject is a diabetic, a smoker, a hemophiliac, an HIV-infected person, an obese person, a person undergoing radiation therapy, or a person with venous stasis ulcer.

10. The method or use according to claim 9, wherein the human subject is a person with venous stasis ulcer.

11. The method or use according to any one of claims 1-10, wherein the composition of step (a) is removed prior to step (b).

12. The method or use according to any one of claims 1-11, wherein the poly--1--4 N-acetylglucosamine is a microalgal poly-- 1--4-N-acetylglucosamine.

13. The method or use according to any one of claims 1-11, wherein the poly--1--4 N-acetylglucosamine is not a crustacean poly- -1-4-N-acetylglucosamine.

14. The method or use according to any one of claims 1-13, wherein the poly--1--4 N-acetylglucosamine fibers have been irradiated to reduce the length of the fibers, and wherein the infrared spectrum of the irradiated poly--1--4-N-acetylglucosamine fibers is substantially similar or equivalent to that of the non-irradiated poly-- 1--4-N-acetylglucosamine fibers.

15. The method or use according to any one of claims 1-14, wherein the composition is in the form of a dressing, a barrier, a membrane, a film, a bandage, a gauze, or a mat.

16. The method or use according to claim 15, wherein the composition is formulated as a dressing, and wherein at least 75% of the dressing consists of the poly--1--4-N acetylglucosamine fibers.

17. The method or use according to any one of claims 1-16, wherein the poly--1-4 N-acetylglucosamine comprises at least 90% of N-acetylglucosamine monosaccharides.

18. The method or use according to any one of claims 1-16, wherein the poly--1-4 N-acetylglucosamine comprises 100% of N-acetylglucosamine mono saccharides.

19. The method or use according to any one of claims 1-18, wherein the poly--1-4 N-acetylglucosamine fibers are from 15 to 150 microns in average length.

20. The method or use according to any one of claims 1-18, wherein the poly--1--4 N-acetylglucosamine fibers are from 20 to 100 microns in average length.

21. The method or use according to any one of claims 1-18, wherein the poly--1--4 N-acetylglucosamine fibers are from 50 to 120 microns in average length.

22. The method or use according to any one of claims 19-21, wherein the length of the fibers is determined by scanning electron microscopic (SEM) analysis.

23. The method or use according to any one of claim 1-22, wherein the biocompatibility of the poly-3-1--4-N-acetylglucosamine fibers is determined by an elution test, intramuscular implantation test, or intracutaneous or systemic injection into animal subjects.

24. The method or use according to claim 23, wherein the biocompatibility is determined by the intramuscular implantation test, wherein the intramuscular implantation test is a 4 week implantation into paravertebral muscle tissue of a rabbit based on International Organization for Standardization Guidelines, and wherein the poly-3-1-4-N-acetylglucosamine fibers are non-reactive when tested in the intramuscular implantation test.

25. The method or use according to any one of claims 1-24, wherein the method is used in conjunction with standard care procedures for chronic wounds.

26. The method or use according to any one of claims 5-7, which is used in conjunction with one or more standard care procedures for cutaneous ulcers, wherein the standard care procedure is: debridement of necrotic or infected tissue; off-loading; compression therapy; establishment of adequate blood circulation; maintenance of a moist wound environment; management of wound infection; wound cleansing; nutritional support; or bowel and bladder care.

27. The method or use according to claim 25 or claim 26, which is used in conjunction with compression therapy.

28. The method or use according to claim 8, wherein the chronic wound is a burn wound, and wherein the method is used in conjunction with one or more standard care procedures for burn wounds, wherein the standard care procedure is: hemodynamic resuscitation; management of comorbidities; timely burn debridement and excision; wound closure; management of wound infection; pain control; nutritional support; measures to inhibit excessive scar formation; or rehabilitation.

29. The method or use according to any one of claims 1-28, wherein said application is repeated at least 4 times.

30. The method or use according to any one of claims 1-29, wherein the composition is formulated as a dressing, and wherein at least 95% of the dressing consists of the poly--1--4 N-acetylglucosamine fibers.

31. The method or use according to any one of claims 1-30, wherein the composition does not comprise cellulose.

32. The method or use according to any one of claims 1-30, wherein the composition does not comprise oxidized cellulose.

33. The method or use according to any one of claims 1-30, wherein the biocompatible poly--1--4-N-acetylglucosamine fibers are the only polymers or fibers in the composition.

34. The method or use according to any one of claims 1-30, wherein the biocompatible poly--1--4-N-acetylglucosamine fibers are the only active ingredient in the composition.

35. The method according to claim 1, substantially as hereinbefore described with reference to any one of the Examples and/or Figures.