AU2006200749A1 - Halogenated selective androgen receptor modulators and methods of use thereof - Google Patents

Description

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Applicant: UNIVERSITY OF TENNESSEE RESEARCH FOUNDATION Invention Title: HALOGENATED SELECTIVE ANDROGEN RECEPTOR MODULATORS AND METHODS OF USE THEREOF The following statement is a full description of this invention, including the best method of performing it known to I HALOGENATED SELECTIVE ANDROGEN RECEPTOR o MODULATORS AND METHODS OF USE THEREOF IEELD OF INVENTION [0001] The present invention relates to a novel class of androgen receptor targeting agents (ARTA), which are selective androgen receptor modulators (SARM). The SARM 0 compounds are useful fora) male contracetion; b) treatment of a variety 'of hormone- S related conditions, for example conditions associated with Androgen Decline in Aging S Male (ADAM), such as fatigue, depression, decreased libido, sexual dysfunction, erectile dysfunction, hypogonadism, osteoporosis, hair loss, anemia, obesity, sarcopenia, osteopenia, osteoporosis, benign prostate hyperplasia, alterations in mood and cognition and prostate cancer; c) treatment of conditions associated With Androgen Decline in Female (ADIF), such as sexual dysfunction, decreased sexual libido, hypogonadism, sarcopenia, osteopenia, osteoporosis, alterations in. cognition and mood, depression, anemia, hair loss, obesity, endometriosis, breast cancer, uterine cancer and ovarian cancer; d) treatment and/or prevention of acute and/or 'chronic muscular wasting S conditions; e) preventing and/or treating dry eye conditions; f) oral androgen replacement therapy; g) decreasing the incidence of thalting or causing a regression of prostate cancer; and/or h) inducing apoptosis in a cancer cell.

BACKGROUND OF TIE INVENTION [0002] The androgen receptor is a ligand-activated transcriptional regulatory protein that mediates induction of male sexual development and function through its activity with endogenous androgens. Androgens are generally known as the male sex hormones. The androgenic hormones are steroids which are produced in the body by the testes and the cortex of the adrenal gland or can be synthesized in the laboratory.

Ahdrogenic steroids play an important role in many physiologic processes, including the development and maintenance of male sexual characteristics such as muscle and bone mass, prostate growth, spermatogenesis, and the male hair pattern (Matsumoto, Endocrinol. Met Clin. N. Am. 23:857-75 (1994)). The endogenous steroidal androgens include testosterone and dihydrotestosterone Testosterone is the principal steroid secreted by the testes and is the primary circulating androgen found in the plasma o of males. Testosterone is converted to DHT by the enzyme 5 alpha-eductase in many C peripheral tissues. DHT is thus thought to serve as the intracellular mediator for most 5 androgen actions (Zhou, et al., Molec. Endocrinol. 9:208-18 (1995)). Other steroidal Cc, androgens include esters of testosterone, such as the cypionate, propionate, phenylpropionate, cyclopentylpropionate, isocarporate, enanthate, and decanoate esters, and other synthetic androgens such as 7-Methyl-Nortestosterone and its acetate ester (Sundaram et al., "7 Alpha-Methyl-Nortestosterone(MENT): The Optimal o 10 Androgen For Male Contraception," Ann. Med., 25:199-205 (1993) ("Sundaram')).

Because the AR is involved in male sexual devlopment and function, the AR is a ikely target for effecting male contraception orpther forms of hormone replacement therapy.

10003] Worldwide population growth and. social awareness of family, planning have stimulated a great deal of research in contracepticeptin. raception is a difficult subject under :any circumstance. It: is .fraught with cultural and social stigma, religious implications, and, most :certainly, ,significant health concerns. This situation is only exacerbated when the subject focuses on male contraception. Despite the availability of suitable contraceptive devices, historically, .society .has looked to. women to be !o responsible for contraceptive decisions- and their consequences.,Although concern over sexually transmitted diseases has made men more aware-of the need to develop safe and responsible sexual habits, women still often bear the brunt of contraceptive choice.

Women have a number of choices, from temporary mechanical devices such as sponges and diaphragms to temporary chemical devices such as spermicides. Women also have at their disposal more permanent options, such as physical devices including IUDs and cervical caps as well as more permanent chemical treatments such as birth control pills and subcutaneous implants. However, to date, the only options available for men include the use of condoms and vasectomy. Condom use, however is not favored by many men because of the reduced sexual sensitivity, the interruption in sexual spontaneity, and the significant possibility of pregnancy caused by breakage or misuse. Vasectomies are also not favored. If more convenient methods of birth control were. available to men, particularly long-term methods which require no preparative activity immediately prior to a sexual act, such methods could significantly increase the likelihood that men would take more responsibility for contraception.

O

0 c [0004] Administration of the male sex steroids testosterone and its derivatives) has 5 shown particular promise in this regard due to the combined gonadotropin-suppressing c and androgen-substituting properties of these compounds (Steinberger et al., "Effect of Chronic Administration of Testosterone Enanthate on Sperm Production and Plasma Testosterone, Follicle Stimulating Hormone, and Luteinizing Hormone Levels: A Preliminary Evaluation of a Possible Male Contraceptive, Fertility and Sterility 28:1320- 28 (1977)). Chronic admiiistration of high doses of testosterone completely abolishes sperm production (az6ospermia) or reduces it to a very low level (oligospermia). The o degree of spennatogenic suppression necessary to produce infertility is not precisely known. However, a recent report by the World Health Organization showed that weeldy intramuscular injections of testosterone enanthate result in azoospennia or severe oligoemia less than 3 million sperm per ml) and infertility in 98% of men reciving therapy (World Health Organization Task Force on Methods And Regulation of Male Fertility, "Contraceptive Efficacy of Testosterone-Induced Azoospermia and Oligospermia in Normal Men," Feftility and Sterility 65:821-29 (1996)).

t0 Q0005] A. variety of testosterone esters have been developed which are more slowly absorbed after intramuscular injection and thus result in greater androgenic effect Testosterone enanthate is the most widely used of these esters. While testosterone enanthate has been valuable in terms of establishing the feasibility of honronal agents for male contraception, it has several drawbacks, including the need for weekly injections and the presence of supraphysiologic peak levels of testosterone immediately following intramuscular injection (Wu, "Effects of Testosterone Enanthate in Normal Men: Experience From a Multicenter Contraceptive Efficacy Study," Fertility and Sterility 65:626-36 (1996)).

[0006) Steroidal ligands which bind the AR and act as androgens testosterone enanthate) or as antiandrogens cyproterone acetate) have been known for many years and are used clinically (Wu 1988). Although nonsteroidal antiandrogens are in clinical use for hormone-dependent prostate cancer, nonsteroidal androgens have not been reported. For this reason, research on male contraceptives has focused solely on o steroidal compounds.

0 [0007] Prostate cancer is one of the most frequently occurring cancers among men in the United States, with hundreds of thousands of new cases diagnosed each year.

c, Unfortunately, over sixty percent of newly diagnosed cases of prostate cancer are found 'to be pathologically advanced, with no cure and a dismal prognosis. One approach to this problem is to find prostate cancer earlier through screening programs and thereby O 10 reduce the number of advanced prostate cancer patients. Another strategy, however, is to CN develop drugs to prevent prostate cancer. One third of all men over 50 years of age have o a latent form of prostate cancer that may be activated into the life-threatening clinical C1 prostate cancer form. The frequency of latent prostati tumors has been shown to increase substantially with each decade of life from the 50s to the 90s The number of people with latent prostate cancer is the same across all cultures, ethnic groups, and races, yet the frequency of clinically aggressive cancer is markedly different. This suggests that environmental factors may play a role in activating latent prostate cancer. Thus, the development.of treatment and preventative,strategies against prostate cancer may have the greatest, overall impact both medically and economically !0 against prostate cancer.

[0008] Osteoporosis is a systemic skeletal diseaseor CHaracterized by low bone mass and deterioration of bone tissue, with a consequent increase in bone fragility and susceptibility to fracture. In the the condition affects more than 25 million people and causes more than 1.3 million fractures each year, including 500,000 spine, 250,000 hip and 240,000 wrist fractures annually. Hip fractures are the most serious consequence of osteoporosis, with 5-20% of patients dying within one year, and over 50% of survivors being incapacitated. The elderly are at greatest risk of osteoporosis, and the problem is therefore predicted to increase significantly with the aging of the population.

Worldwide fracture incidence is forecasted to increase three-fold over the next 60 years, and one study estimated that there will be 4.5 million hip fractures worldwide in 2050.

[0009] Women are at greater risk of osteoporosis than men. Women experience a sharp acceleration of bone loss during the five years following menopause. Other factors that iO increase the risk include smoking, alcohol abuse, a sedentary lifestyle and low calcium S intake. However, osteoporosis also occurs frequentlyin males. It is.well established that rC 5 the bone mineral density of males decrease with age. Decreased amounts of bone -L mineral content and density correlates with decreased bone strength, and predisposes to fracture. The molecular mechanisms underlying the pleiotropic effects of sex-hormones in non-reproductive tissues are only beginning to be understood, but it is clear that physiologic concentrations of androgens: and estrogens play an important role in maintaining bone homeostasis throughout the life-cycle. Consequently, when androgen S or estrogen deprivation occurs there is a resultant increase in the rate of bone remodeling O that tilts the .balance of resorption and formation to the favor of resorption that Scontributes to the overall loss of bone mass. In males, the natural decline in sexhormones atmaturity (direct decline in androgens as well as lower levels of estrogens derived from peripheral aromatization of androgens) is associated with the frailty of bones. This effect is also observed in males who have been castrated.

[00010] Androgen decline in the aging male (ADAM) refers to a progressive decrease in androgen production, common in males after middle age. The syndrome is characterized 0 by: alterations in the physical and intellectual .domains that correlate with and can be corrected'.by manipulation of the androgen milieu. ADAM is characterized biochemically by a decrease not only in serum androgen, but also in other hormones, such as growth hormone, melatonin and dehydroepiandrosterone. Clinical manifestations include fatigue, depression, decreased libido, sexual dysfunction, erectile dysfunction, hypogonadism, osteoporosis, hair loss, obesity, sarcopenia, osteopenia, benign prostate hyperplasia, and alterations in mood and cognition.

l00011]Androgen Deficiency in Female (ADIF) refers to a variety of hormone-related conditions including, common in females after middle agest. The syndrome is characterized by sexual dysfunction, decreased sexual libido, hypogonadism, sarcopenia, osteopenia, osteoporosis, alterations in cognition and mood, anemia, depression, anemia, hair loss, obesity, endometriosis, breast cancer, uterine cancer and ovarian cancer.

[00012]Muscle wasting refers to the progressive loss of muscle mass and/or to the 0 progressive weakening and degeneration of muscles, including the skeletal or voluntary CNl muscles, which control movement, cardiac muscles, which control the heart q 5 (cardiomyopathics), and smooth muscles. Chronic muscle wasting is a chronic condition persisting over a long period of time),characterized by progressive loss of (N muscle mass, weakening and degeneration of muscle. The loss of muscle mass that occurs during muscle wasting can be characterized by a muscle protein breakdown or degradation. Protein degradation occurs because of an unusually high rate of protein o0 i degradation, an unusually low rate of protein synthesis, or a combination of both.

N Protein degradation, whether caused by a high degree of protein degradation or a low o degree of protein synthesis, leads to a decrease in muscle mass and to muscle wasting.

(N Muscle wasting is associated with chronic,' neurological, genetic or infectibus pathologies, diseases, illnesses or conditions. These include Muscular Dystrophies such as Duchenne Muscular Dystrophy and Myotonic Dystrophy; Muscle Atrophies such as Post-Polio Muscle Atrophy (PPMA); Cachexias such as Cardiac Cachexia, AIDS Cachexia and Cancer Cachexia, malnutrition, Leprosy, Diabetes, Renal Diseaseor CHronic Obstructive Pulmonary Disease (COPD), Cancer, end stage Renal failure, Emphysema, Osteomalacia, HIV Infection, AIDS, and Cardiomyopathy, In addition, !0 other circumstances and conditions are linked to and can cause muscle wasting. These include, chronic lower back pain, advanced age, central nervous system (CNS) injury, peripheral nerve injury, spinal cord injuryor Chemical -injury, central nervous system (CNS) damage, peripheral nerve damage, spinal cord damageor CHemical damage, burns, disuse deconditioning that occurs when a limb -is immobilized, long term hospitalization due to illness or injury, and alcoholism. Muscle wasting, if left unabated, can have dire health consequences. For example, the changes that occur during muscle wasting can lead to a weakened physical state that is detrimental to an individual's health, resulting in increased susceptibility to infection, poor performance status and -susceptibility to injury.

[00013]New innovative approaches are urgently needed at both the basic science and clinical levels to develop compounds which are useful for a) male contraception, b) treatment of a variety of hormone-related conditions, for example conditions associated with Androgen Decline in Aging Male (ADAM), such as fatigue, depression, decreased o libido, sexual dysfunction, erectile dysfunction, hypogonadism, osteoporosis, hair loss, anemia, obesity, sarcopenia, osteopenia, osteoporosis, benign prostate hyperplasia, alterations in mood and cognition and prostate cancer, c) treatment of conditions associated with ADIP, such as sexual dysfunction, decreased sexual libido, hypogonadism, sarcopenia, osteopenia, osteoporosis, alterations in cognition and mood, depression, anemia, hair loss, obesity, endometriosis, breast cancer, uterine cancer and ovarian cancer; d) treatment and/or prevention of acute and/or chronic muscular wasting o 10 conditions; e) preventing and/or treating dry eye conditions; f) oral androgen replacement therapy; and/or g) decreasing the incidence of, halting or causing a regression of prostate cancer.

SUMMARY OF TBE INVENTION [00014]This invention provides a class of androgen receptor targeting agents (ARTA).

The agents defime a new subclass of compounds, which are selective androgen receptor modulators (SARM). Several of the SARM compounds have been found to have an unexpected androgenic and anabolic activity of a nonsteroidal ligand for the androgen receptor. Other SARM compounds have been found to have an unexpected antiandrogenic activity of a nonsteroidal ligand for the androgen receptor. The SARM compounds, either alone or as a composition, are useful for a) male contraception; b) treatment of a variety of hormone-related conditions, for example conditions associated with Androgen Decline in Aging Male (ADAM), such as fatigue, depression, decreased libido, sexual dysfunction, erectile dysfunction, hypogonadism, osteoporosis, hair loss, anemia, obesity, sarcopenia, osteopenia, osteoporosis, benign prostate hyperplasia, alterations in -mood and cognition and prostate cancer, c) treatment of conditions associated with Androgen Decline in Female (ADIF), such as sexual dysfunction, decreased sexual libido, hypogonadism, sarcopenia, osteopenia, osteoporosis, alterations in cognition and mood, depression, anemia, hair loss, obesity, endometriosis, breast cancer, uterine cancer and ovarian cancer; d) treatment and/or prevention of acute and/or chronic muscular wasting conditions; e) preventing and/or treating dry eye conditions; f) oral androgen replacement therapy; g) decreasing the incidence of halting or causing a IDregression of prostate cancer; and/or h) inducing apoptosis in a cancer cell.

0 0 [0001 ol5]In one embodiment, the present invention provides a selective androgen receptor modulator (SARM) compound represented by the strture of formula (Ha):

NH

Z -Q 0Y \O yr 0 o wherein to Xis O, CH2, NH, Se, PR, NO orNR; T is OH, OR, -NHCOCH 3 or NHCOR; Z is NO 2 CN, COOH, COR, NHCOR or CONHR; Y is CF3, 3 Br, C 1 CN, CR 3 or SnR 3 or Z and Y together with the benzene ring to which they are attached form a fused bicyclic carbocyclic or heterocyclic ring system; Q is F, Cl, Bror R is alkyl, haloalkyl, dihaloalkyl, trihaloalkyl, CH2F, CBF 2

CF

3

CF

2

CF

3 aryl, phenyl, F, Cl, Br, I, alkenyl or OH; and RI is CH 3 CItF, CIF 2 CF3, CH 2 CH3, or CF 2

CF

3 with the proviso that when Q is F, Z is not NO2. [00016] In another embodiment, the present invention provides an analog, isomer, metabolite,- derivative, pharmaceutically acceptable salt, pharmaceutical product hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the coinpound of formula or any combination thereof.

[00017] In one embodiment of formula Q is F. In another embodiment of formula (HIa), Q is CL In another embodiment of formula Q is Br. In another embodiment of formula Q is I.

[00018]In one embodiment, X in compound (Ha) is 0. In another embodiment, Z in compound (Ia) is NO 2 In another embodiment, Z in compound (IIa) is CN. In another embodiment, Z in compound (Ha) is NO 2 with the proviso that Q is not F. In another embodiment Y in compound (Ila) is CF 3 In another embodiment, T in compound is OH. In another embodiment, Ri in compound (Ha) is CH 3 [00019]In another embodiment, the compound of formula (Ha) is represented by the structure: 0 2 N C 0 1 Th .0 [00020]In another embodiment, the compound of formula (Ha) is represented by the structure: 02N. Br [00021] n another embodiment the compound of formula (Ha) is represented by the structure: [00022]In another embodiment the compound of formula (Ia) is represented by the structure: [00023]In another embodiment, the compound of formula (Ha) is represented by the stnuctre:

NC

[00024]In another embodiment ,the compound of formula (Ha) is represented by the structure: [00025]In another embodiment the compound of formula (la) is represented by the structure: [00026]In another embodiment, the present invention provides a selective androgen receptor modulator (SARM) compound represented by the structure of formula (Hb): Va 0 H 3 C OH 3NH -z N

Q

cK

Y

Ib 0\ wherein 0X is O, CH2, N9, Se, PR, NO orN1, T is OH, OR, -NHCOCH 3 or NHCOR; Zis H,F C Lror i; c 10 Y is CF 3 1, Br, Cl, CN, CR 3 or SnR3; Q is alkyl, F, I, Br, Cl, CF 3 CN CR 3 SnR 3

NR,

NHCOCH3, NHCOCF3, iNHCO, qHCONH 2

NHCONHR,

NHCONR

2 NHCOOR, OCONHR, CONHR, NHCSCH 3

NHCSCF

3 NHCSR, NHCSNI 2 NHCSNHR, NHCSNR 2 NHS02CH 3

NHSO

2 R, OR, COR, OCOR, OS02R, S0 2 R, SR; or Q together with the benzene ring to which it is attached is a fused ring system represented by structure A, B or C: cro Q of A B C R is alkyl, haloalkyl, dihaloalkyl, trilaloalkyl, CH 2 F, CB 2

CF

3

CF

2

CF

3 aryl, phenyl, F, CL, Br, I, alkenyl or OH; and R is CH 3 CH2E, CI 2

CF

3

CH

2

CH

3 or CF 2

CF

3 100027] In another embodiment, the present invention provides an analog, isomer, metabolite, derivative, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the compound of formula (fib), or any combination thereof.

-*Q

[00028] In one embodiment of formula (lib), Z is H. In another embodiment of formula (IIb), Z is F. In another embodiment of formula (IIb), Z is CI. In another embodiment ci of formula Z is Br. In another embodiment of formula (IIb), Z is I.

[00029] In one embodiment, X in compound (I1b) is O. In another embodiment, Y in cN compound is CF 3 In another embodiment, T in compound (lib) is OH. In another embodiment, Ri in compound (lib) is CH 3 In another embodiment, Q in compound (lib) is F. In another embodiment, Q in compound (fib) is CL In another embodiment, Q in 0to compound (lib) is Br. In another embodiment, Q in compound (lib) is I. In another C embodiment, Q in compound (lib) is in the para position. In another embodiment, Z in S compound (flb) is in the para position. In another embodiment, Y in compound (Jib) is Ci in the meta position.

[00030]In another embodiment, the compound of formula (IIb) is represented by the structure: Cl

F

H

3 C OH [O0031]In another embodiment, the compound of formula (Ib) is represented by the structure: F F 0

N

CF3 O

H

3

O

100032]In another, embodiment, the compound of formula (Ilb) is represented by the structure:

-F

[00033]In another embodiment, the compound of formula (fib) is represented by the structure: [aao00034]In another embodiment, the compound:of formula (ib) is represented by the structure: 1000351n another embodiment, the compound of formula (fIb) is represented by the structFe:

F

[00036]n another embodimient, the compound of. formula 6Tb) is represented by the structure: [Ooo3jIn another embodiment, the present invention.provides a selective androgen receptor modulator (SARM).compoundrepresented by the structure:

ID

SNC CN and/or its analog, isomer, metabolite, derivative, pharmnnaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the compound of formula (IIb), or any combination thereof.

0 C [00038]In another embodiment, the present invention provides a selective androgen O receptor modulator (SARM) compound represented by the stuctire: NC NC F Cl 1 o and/or its analog, isomer, metabolite, derivative, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the compound of formula (fib), or any combination thereof.

[00039]In one embodiment, the SARM compound of any of the above formulas is an audrogen receptor agonist.

[00040] In one embodiment, the present invention provides a composition comprising the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof; and a suitable carrier or diluent.

[00041] In another embodiment, the present invention provides a pharmaceutical composition comprising the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutical product, hydrate or N-oxide or any combination thereof, and a suitable carrier or diluent.

[00042]Iu another embodiment, the present invention provides a method of binding a I selective androgen receptor modulator compound to an androgen receptor, comprising the step of contacting the androgen receptor with the selective androgen receptor ,C 5 modulator compound of the present invention and/or its analog, derivative, isomer, Smetabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, c prodnug, polymorph, crystal or any combination thereof, in an amount effective to bind the selective androgen receptor modulator compound to the androgen receptor.

10 [00043]In anothet embodiment the present invention provides a method of suppressing 0 spenmatogenesis in a subject comprising contacting an androgen receptor of the subject S with the selective androgen receptor modulator compound of the present invention c- and/or its analog, derivative, isomer, metabolite, phatmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to suppress sperm production.

[00044] hn another embodiment, the present invention provides a method of contraception in a male subject, comprising the step of administering to the subject the selective androgen receptor modulator compound of the present invention and/or its w0 analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to suppress sperm production in the subject, thereby effecting contraception in the subject.

[00045] In another embodiment, the present invention provides a method of hormone therapy comprising the step of contacting an androgen receptor of a subject with the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to effect a change in an androgen-dependent condition.

[00046]In another embodiment, the present invention provides a method of hormone replacement therapy comprising the step of contacting an androgen receptor of a subject o with the selective androgen receptor modulator compound of the present invention S and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, 5 pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof in an amount effective to. effect a change in an androgen-dependent condition.

[00047JIn another embodiment, the present invention provides a method of treating a subject having a hormone related condition, comprising the step of administering to the 0 subject the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to effect a change in an androgen-dependent condition.

[00048]In another embodiment, the present invention provides a method of treating a subject suffering from prostate cancer, comprising the step of administering to said subject the selective androgen receptor modulator compound of the present invention o and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to treat prostate cancer in the subject.

[00049]In another embodiment, the present invention provides a method of preventing prostate cancer in a subject, comprising the step of administering to the subject the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, phannaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to prevent prostate cancer in the subject.

00050] In another embodiment, the present invention provides a method of delaying the progression of prostate cancer in a subject suffering from prostate cancer, comprising the step of administering to said subject the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, I pharmaceutically acceptable salt, phanraceutical product, hydrate, N-oxide, prodrug, 0 polymorph, crysta or any combination thereof, in an.amount effective to delay the r3 5 progression of prostate cancer in the subject.

[00051]In another embodiment, the present invention provides a method of preventing the recurrence of prostate cancer in a subject suffering from prostate cancer, comprising the step of administering to said subject the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, 0 pharmaceutically acceptable salt, pharnaceutical product, hydrate, N-oxide, prodrug, o polymorph, crystal or any combination thereof, in an amount effective to prevent the O0 0 recurrence of prostatecancer in the subject [000521 In another embodiment, the present invention provides a method of treating the recurrence of prostate cancer in a subject suffering from prostate cancer, comprising the step of administering .to said subject, the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to treat the recurrence of prostate cancer in the subject.

-00053] n another embodiment, the present invention provides a method of treating a dry eye condition.in a subject suffering from dry eyes, comprising the step of contacting an androgen receptor of a subject with the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to treat dry eyes in the subject. [00054] In another embodiment, the present invention provides a method of preventing a dry eye condition in a subject, comprising the step of contacting an androgen receptor of a subject with the selective .androgen receptor modulator compound of the present \O invention and/or its analog, derivative, isomer, metabolite, phamaceutically acceptable

O

0 salt, pharmaceutical product, hydrate, N-oxide, prodrug, polmorph, crystal or any combination thereof, in an amount effective to prevent dry eyes in the subject.

FI Cc¢ [00055]In another embodiment, the present invention provides a a method of inducing apoptosis in a cancer cell, comprising the step of contacting the cell with with the selective androgen receptor modulatori compound of the present invention and/or its r analog, derivative, isomer, metabolit, pharmaceutically acceptable salt, pharmaceutical o o product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an IN amount effective to induce apoptosis in the cancer cell.

[00056]The novel selective androgen receptor modulator compounds of the present invention, either alone or as a pharmaceutical composition, are useful for a) male contraception; b) treatment of a variety of hormone-related conditions, for example conditions associated with ADAM, such as fatigue, depression, decreased libido, sexual dysfunction, erectile dysfunction, hypogonadism, osteoporosis, hair loss, obesity, sarcopenia, osteopenia, benign prostate hyperplasia, and alterations in mood and cognition; c) treatment of conditions associated with ADIF, such as sexual dysfunction, o decreased sexual libido, hypogonadism, sarcopenia, osteopenia, osteoporosis, alterations in cognition and mood, depression, anemia, hair loss, obesity, endometriosis, breast cancer, uterine cancer and ovarian cancer; d) treatment and/or prevention of acute and/or chronic muscular wasting conditions; e) preventing and/or treating dry eye conditions; f) oral androgen replacement therapy; g) decreasing the incidence of, halting or causing a regression of prostate cancer; and/or h) inducing apoptosis in a cancer cell [00057]The selective androgen receptor modulator compounds of the present invention offer a significant advance over steroidal androgen treatment. Several of the selective androgen receptor modulator compounds of the present invention have unexpected androgenic and anabolic activity of a nonsteroidal ligand for the androgen receptor.

Other selective androgen receptor modulator compounds of the present invention have unexpected antiandrogenic activity of a nonsteroidal ligand for the androgen receptor.

Thus, treatment with the selective androgen receptor modulator compounds of the present invention will not be accompanied by serious. side effects, inconvenient modes Sof administration, or high costs and will still have the advantages of oral bioavailability, o lack of cross-reactivity with other steroid receptors, and long biological half-lives.

0 BRIEF DESCRIPION OF THM FIGURES c [00058] The present invention will be understood and appreciated more fully from the following detailed description taken in conjunction with the appended figures which depict: 0 [00059] Figure 1: Androgenic and Anabolic activity of Compounds 1-4. Rats o were left untreated (intact control), castrated (0 mg/day control), or treated with 0.1, 0.3, (c 0.5, 0.75 and 1.0 mg/day of compound 1 (Fig 1A), compound 2 (Fig 1B), compound 3 (Fig IC) or compound 4 (Fig ID), and the weight of androgen-responsive tissues (prostate, semimal vesicles and levator ani muscle) was determined.

[00060] Figure 2: Androgenic and Anabolic activity of Compound 5. .Rats were left untreated (intact control), castrated (0 mg/day control), or treated with 0.1, 0.25, 0.75 and 1.0 mg/day of compound 5, and the weight of androgen-responsive tissues o (prostate, semimal vesicles and levator ani muscle) was determined.

[00061] Figure Androgenic and Anabolic activity of Compound 6 in rats. Rats were left untreated (intact control), castrated (castrated control), treated with 0.1, 0.3, 0.75 and 1.0 mg/day testosterone propionate or treated with 0.1, 0.3, 0.5, 0.75 and 1.0 mg/day Compound V, and the weight of androgen-responsive tissues (prostate, semimal vesicles and levator ani muscle) was determined.

[00062] Figure 4: Androgenic and Anabolic activity.of Compound, 1 in rats. Rats were left untreated (intact control), castrated (castrated control), treated with 0.1, 0.3, 0.5, 0.75 and 1.0 mg/day testosterone propionate or treated with 0.1, 0.3, 0.5, 0.75 and 1.0 mg/day Compound 1, and the weight of androgen-responsive tissues (prostate, semimal vesicles and levator ani muscle) was determined.

[00063] Figure 5: Effects of Compound 1 and Compound 21 on LH Levels.

C [00064] Figure 6: Effects of Compound 1 and Compound 21 on FSH Levels.

So[00065] Figure 7: Synthesis scheme of Compound 21.

DETAILED DESCRIPTION OF THE INVENTIO r- [00066] In one embodiment, this invention provides a class of androgen receptor I\N targeting agents (ARTA). The agents defin a new subclass of compounds, which are o selective androgen receptor modulators (SARM). Several of the SARM compounds have been found to have an unexpected androgenic and anabolic acivity of a nbnsteroidal ligand for the androgen receptor. Other SARM compounds have been found to have an unexpected antiandrogenic activity of a honsteroidal ligand for the androgen receptor.

The SARM compounds, either alone or as a composition, are useful for a) male contraception; b) treatment of a variety of hormone-related conditions, for example conditions associated with Androgen Decline in Aging Male (ADAM), such as fatigue, depression, decreasedlibido, sexual dysfunction, erectile dysfunction, hypogonadism, osteoporosis, hair loss, anemia, obesity, sarcopenia, osteopenia, osteoporosis, benign prostate hyperplasia, alterations in mood and cognition and prostate cancer; c) treatment of conditions associated with Androgen Decline in Female (ADIF), such as sexual dysfunction, decreased sexual libido, hypogonadism, sarcopenia, osteopenia, osteoporosis, alterations in cognition and mood, depression, anemia, hair loss, obesity, endometriosis, breast cancer, uterine cancer and ovarian cancer; d) treatment and/or prevention of acute and/or chronic muscular wasting conditions; e) preventing and/or treating dry eye conditions; f) oral androgen replacement therapy; g) decreasing the incidence of, halting or causing a regression of prostate cancer; and/or h) inducing apoptosis in a cancer cell.

[00067]In one embodiment, the present invention provides a selective androgen receptor 0 modulator (SARM) compound represented by the structure offomnula (Ha); .n RI> T SIa 'Wherein 0 SXis O, CH2, NH, Se, PR, NO or NR IN T is OH, OR, -NHCOCH3, or NHCOR; Z is NO 2 CN, COOH, COR, NHCORor CONHR; Y is CF 3 ,,Br,CI, CN, CR 3 orSnR 3 or Z and Y together ith the benzene ring to which they are attached form a fused bicyclic carbocyclic or heterocyclic ring system; Q isF,C1,BrorI; R is'alky, haloalcyl, dihaloalkylt 1ihaloal, CH 2 F, CIF 2

CF

3

CF

2 CF3, aryl, phenyl, F, Cl,'Br, I, alkenyl or OH; and RI is CH3, CH 2 F, CHF 2 CF3, CH2CH3, or CF2CF 3 with the proviso that when Q is F, Z is not NO2.

[000681 In one embodiment this invention provides an analog of the compound of formula In another embodiment, this invention provides a derivative of the compound of formula In another embodiment, this invention provides an isomer of the compound of formula In another embodiment, this invention provides a metabolite of the compound of formula In another embodiment, this invention provides a pharmaceutically acceptable salt of the compound of formula In another embodiment, this invention provides a pharmaceutical product of the compound of formula In another embodiment, this invention provides a hydrate of the compound of formula In another embodiment, this invention provides an N-oxide. of the compound of formula In another embodiment, this invention provides an impurity of the compound of formula In another embodiment, this invention provides a prodrug of the compound of formula In another embodiment, this invention S provides a polymorph of the compound of formula In another embodiment, this oinvention provides a crystal of the compound of formula In another embodiment, this invention provides a combination of any of an analog, derivative, metabolite, ec isomer, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the compound of formula (la).

[00069] In one embodiment of formula Q is F. In another embodiment of formula Q is Cl. In another embodiment of formula Q is Br. In another embodiment of Sformula Q is I.

[00070]In one embodiment, X in compound (Ia) is O. In another embodiment, Z in compound (la) is NO 2 In another embodiment, Z in compound (la) is CN. In another embodiment, Z in compound (Ia) is NOz, with the proviso that Q is not F. In another embodiment, Y in compound (la) is CF 3 In another embodiment, T incompound is OH. In another embodiment, RI in compound (Ia) is CH3. In another embodiment, Q in compound (Ia) is in the para position. In another embodiment, Z in compound (Ia) is in the para position. In another embodiment, Y in compound (la) is in the meta position.

[00071]In one embodiment, the compound of formula (la) is represented by the structure of formula (Ha): H3C OH nc oi -0 a wherein Z, Y and Q are as defined above for formula [00072]In another embodiment, the compound of formula (Ia) is represented by the structure: [oo073]In another embodiment, the compound of formula (Ia) is represented by the structure: 02N Br C F 3 i H3C OH [00074]In another embodiment, the compound of formula (la) is represented by the structure: N0 0

CF

3

H

[00075]In another. embodiment, the compound of formula (Ta) is represented by the structure: NC

F

CF

3 0 1H13' [00076]n another embodiment, the compound of formula (Ia) is represented by the structure: [00077]3n another embodiment the compound of fomula (Ia) is represented by the structure: [00078]In another embodiment, the compound of formula (Ia) is represented by the structure:

NC

HCC Qf [00079]In another embodiment, the -present invention provides a selective androgen receptor modulator (SARM) compound represented by the structure of formula

\O

Va 0 ~~0 Ib wherein X is O, CH 2 3 NH Se, PR, NO or NR; o T is Oh OR, -NHCOCH 3 or NHCOR; IN Z is H F, C, Br or I; O o Y is CF 3 I, Br, Cl, CN, CR 3 or SnR 3 Q is alkyl, F, I, Br, Ci, CF 3 CN CR 3 SnR 3

NR

2

NHCOCH

3 NHCOCF, NHCOR, NHCONI 2

NHCONHR,

NHCONR

2 NHCOOR, OCONR CONHR, NHCSCH,

NHCSCF

3 NHCSR, NHCSNH 2 NHCSNHIR, NICSNR 2 NHS02CH3, NHSO 2 R, OR, COR, OCOR, OS02R, S02R, SR; or Q together with the benzene ring to which it is attached is a fused ring system represented by structure A, B or C: NE

NH

A B C R is alkyl, haloalkyl, dihaloallEcyl, trihaloalkyl, CH2F, CBF 2

CF

3

CF

2

CF

3 aryl, phenyl, F, Cl, Br, alkenyl or OH; and R is CH 3 CH2FH, C, F 3 CH12CH3, or CF 2

CF

3 (00080] In one embodiment, this invention provides an analog of the compound of formula In another embodiment, this invention provides a derivative of the compound of formula In another embodiment this invention provides an isomer of the compound of formula In another embodiment, this invention provides a metabolite of the compomund of formula In another embodiment, this invention provides a pharmaceutically acceptable salt of the compound of formula In another embodiment, this invention provides a pharmaceutical product of the compound of S formula In another embodiment, this invention provides a hydrate of the compound 0 of formula In another embodiment, this invention provides an N-oxide of the compound of formula In anoher embodimentt, this invention provides an N-oimpurityde of the of th compound of fo rmula In another embodiment, this invention provides an impurity of the compound of formula In another embodiment, this invention provides a prodrug of the compound of formula In another embodiment, this invention provides a polymorph of the compound of formula In another embodiment, this invention provides a crystal of the compound of formula In another embodiment, this invention provides a combination of any of an analog, derivative, metabolite, isomer, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the compound of formula (Ib).

[00081] In one embodiment of foraula Z is H. In another embodiment of formula Z is F. In another embodiment of formula Z is Cl. In another embodiment of formula Z is Br. In another embodiment of formula Z is I.

[00082 In one embodiment, X in compound (Ib) is 0. In another embodiment, Y in compound is CF 3 In another embodiment, T in compound (Ib) is OH. In another embodiment, Ri in compound (Ib) is CH 3 In another embodiment, Q in compound (Ib) is F. In another embodiment, Q in compound (Ib) is Cl. In another embodiment, Q in compound (Ib) is Br. In another embodiment, Q in compound (Ib) is I. In another embodiment, Q in compound (Ib) is in the para position. In another embodiment, Z in compound (Ib) is in the para position. In another embodiment, Y in compound (Ib) is in the meta position.

[00083]In one embodiment, the compound of formula (1b) is represented bythe structure of formula flb: Ba 3Q llb wherein Z, Y and Q are as defined above for formula (Ib).

[00084]In another embodiment, the compound of formula (Ib) is represented by the structure: -3l; un another embodiment, the compound of formula (Ib) is represented by the structure: [00086]In another embodiment, the compound of formula (Ib) is represented by the structure; Ik p [00087]In another embodiment, the compound of formula (Ib) is represented by the structure: [00086]In another embodiment, the compound of fonmula (Ib) is represented by the structure: S C' "tOH [o0089]In another embodiment, the compound of formula (Ib) is represented by the structue:, [00090In another embodiment, the compound of fornula (Ib) is represented by the structure: Br F [00091]]n another embodiment, the present invention provides a selective androgen receptor modulator (SARM) compound represented by the structure of formula (Ic): 0 Q R cl wherein X is O, CH2, NH, Se, PR, NO or NR; ST is OH, LR, -NHCOCH 3 or NHCOR; SZ is NOz, CN OO, COR, NHCOR or CONHR; Y is F, Cl, Br or I; O Q is F, CI, Br or I; o 10 R is alkyl, haloakyl, dihaloalkyl, taloalkyl, CH 2 F, CHF 2 CF3, CF 2

CF

3 aryl, phenyl, F, Cl, Br, I, alkenyl or OH; and Ri is CH3, CH 2 F, CHF 2 CF3, CH 2

CH

3 or CF2CF3 [00092] In one embodiment, this invention provides an analog of the compound of formula In another embodiment, this invention provides a derivative of the compound of formula In another embodiment, this invention provides an isomer of the compound of formula In another embodiment, this invention provides a metabolite of the compound of formula In another embodiment, this invention provides a pharmaceutically acceptable salt of the compound of formula In another embodiment, this invention provides a pharmaceutical product of the compound of formula In another embodiment, this.invention provides a hydrate of the compound of formula In another embodiment, this invention provides an N-oxide of the compound of formula In aiother embodiment, this invention provides an impurity of the compound of formula In another embodiment, this invention provides a prodrug of the compound of formula In another embodiment, this invention provides a polymorph of the compound of formula In another embodiment this invention provides a crystal of the compound of formula In another embodiment, this invention provides a combination of any of an analog, derivative, metabolite, isomer, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the compound of formula (Ic).

[00093] In one embodiment of formula Y is F. In another embodiment of formula o Y is Cl. In another embodiment of formula Y is Br. In another embodiment of Cl formula Y is I. In another embodiment of formula Q is F. In another o 5 embodiment of formula Q is Cl. In another embodiment of formula Q is Br.

In another embodiment of formula Q is I.

[00094] In one embodiment of formula Y is F and Z is F. In one embodiment of formula Y is F and Z is Cl. In one embodiment of formula Y is F and Z is Br.

io In one embodiment of formula Y is F and Z is I.

(00095] In one embodiment of formula Y is Cl and Z is F. In one embodiment of formula Y is Cl and Z is Cl. In one embodiment of formula Y is CI and Z is Br.

In one embodiment of formula Y is Cl and Z is I: [00096] In one embodiment of formula Y is Br and Z is F. In one embodiment of formula Y is Br and Z is Cl. In one embodiment of formula Y is Br and Z is Br. In one embodiment of formula Y is Br and Z is I.

[00097] In one embodiment of formula Y is I and Z is F. In one embodiment of formula Y is I and Z is CL In one embodiment of formula Y is I and Z is Br. In one embodiment of formula Y is I and Z is I.

.[0098]In one embodiment, X in compound (Jo) is 0. In another embodiment, Z in compound (Ic) is NO 2 In another embodiment, Z in.compound (Ic) is CN. In another embodiment, T in compound (Ic) is OH. In another embodiment, Ri in compound (Ic) is

CH

3 In another embodiment, Q in compound (Ic) is in the para position. In another embodiment, Z in compound (Ic) is in the para position. In another. embodiment, Y in compound (Ic) is in the meta position.

[00099] In one embodiment, the compound of formula (Ic) is represented by the structure of formula (He):

VD

O

0 0 LI v

I

Y

Ic wherein Z, Y and Q are as defined above for fonnula (Ic).

[000100] In another embodiment, the compound of formula (Ic) is represented by the structure: [000101] the structure: In another embodiment, the compound of formula (Ic) is represented by o

H

3

C.O

ci

C)

M [000102j In one embodiment, the present invention provides a selective androgen receptor modulator (SARM) compound represented by the structure of formula (Id): 00 \O Id wherein X is O, CH2, NL, Se, PR, NO, or NR; T is OH, OR, -NHCOCH 3 or NHCOR; Z is H, F, I, Br, Cl, NO 2 CN, COOH, COR, NHCOR or

CONHER

Y is CF, F, I, Br, CI, CN, CR3 or SnRa; or Z and Y together with the benzene ring to which they are attached form a fused bicyclic carbocyclic or heterocyclic ring system; Q is allyl I, Br, C1, CF 3 CN CR 3 SnR 3

NR

2

NHCOCH

3

NFICOCF

3 NHCOR,

NHCONH

2

NHCONIIR,

NHCONR

2 NHCOOR, OCONIR, CONHR, NHCSCH 3

NHCSCF

3 NHCSR,

NHCSNH

2 NHCSNHR,

NHCSNR

2

NHSO

2

CH

3 NHS0 2 R, OR, COR, OCOR, OS02R, SO 2 R, SR; or Q together with the benzene ring to which it is attached is a fused ring system represented by struture A, B or C: A B C A 1 Ris alyl, haloalyl, dihaloalkyl, trihaoalkyl, CH2F, CHF 2 S CF3, CF2CF 3 aryl, phenyl, F, Cl, Br, I, alkenyl or OH; and c R 1 is CH 3

CH

2 F, CHF 2

CF

3

CH

2

CH

3 or CF 2

CF

3 C) (000103] In one. embodiment, this invention provides an analog of the compound of c formula In another embodiment, this invention provides a derivative of the compound of formula In another embodiment, this invention provides an isomer of the compound of formula In another embodiment this invention provides a O 10 metabolite of the compound of formula In another embodiment, this invention Cl provides a pharmaceutically acceptable salt of the compound of formula In another S embodiment, this invention provides a pharmaceutical product of the compound of cl formula In another embodiment, this invention provides a hydrate of the compound of formula In another embodiment, this invention provides an N-oxide of the compound of formula In another embodiment, this invention provides an impurity of the compound of formula In another embodiment, this invention provides a prodrug of the compound of formula In another embodiment, this invention provides a polymorph of the compound of formula In another .embodiment, this invention provides a crystal of the compound of formula In another embodiment, this invention provides a combination of any of an analog, derivative, metabolite, isomer, pharmaceutically acceptable salt pharmaceutical product, hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the compound of formula (Id).

[000104] In one embodiment, X in compound (Id) is O. In another embodiment Z in compound (Id) is NO 2 In another embodiment, Z in compound (Id) is CN. In another embodiment, Z in compound (Id) is H. In another embodiment Z in compound (Id) is Cl. In another embodiment, Z in compound (Id) is Br. In another embodiment, Z in compound (id) is F. In another embodiment, Z in compound (Id) is I. In another embodiment, Y in compound (Id) is CF 3 In another embodiment, Y in compound (Id) is C1. In another embodiment, Y in compound (Id) is Br- In another embodiment Y in compound (Id) is F. In another embodiment, Y in compound (Id) is I. In another embodiment, Q in compound (Id) is NHCOCH 3 In another embodiment, Q in compound (Id) is F. In another embodiment, Q in compound (Id) is Cl. In another IN embodiment, Q in compound (Id) is Br. In another embodiment, Q in compound (Id) is o I. In another embodiment, T in compound (Id) is OH. In another embodiment, R in compound (Id) is CH 3 en [000105] In another embodiment, the present invention provides a selective androgen receptor modulator (SARM) compound represented by the structure of formula C\ (Hfd):

H

3 C. O o Ild wherein Z, Y and Q are as represented above for compound (1c).

[000106] In one embodiment, this invention provides an analog of the compound of formula In another embodiment, this invention provides a derivative of the compound of formula (IId). In another embodiment, this invention provides an isomer of the compound of formula In another embodiment, this invention provides a metabolite of the compound of formula In another embodiment, this invention provides a pharmaceutically acceptable salt of the compound of formula In another embodiment, this invention provides a pharmaceutical product of the compound of formula (IId). In another embodiment, this invention provides a hydrate of the compound of formula In anoter embodiment, Itis invention provides an N-oxide of the compound of formula (Hld). In another embodiment, this invention provides an impurity of the compound of formula In another embodiment, this invention provides a prodrug of the 'compound of formula In another embodiment, this invention provides a polymorph of the compound of formula In another embodiment, this invention provides a crystal of the compound of formula In another embodiment, this invention provides a combination of any of analog, derivative, metabolite, isomer, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, impurity, prodrug, polymorph or crystal of the compound of formula (lId).

cN [000107] In one embodiment, X in compound (Hd) is O. In another embodiment,

Z

in compound is NO 2 In another embodiment, Z in compound (Hd) is CN. In another c embodiment, Z in compound (Hd) is I. In another embodiment, Zin compound (ld) is Ci Cl. In another embodiment Z in compound (ld) is Br. In another embodiment, Z in compound (ld) is F. In another embodiment, Z in compound (1d) is I. In another embodiment, Y in compound (lid) is CF 3 In another embodiment, Y in compound is Cl. In another embodiment, Y in compound (IGd) is Br. In another embodiment, Y in c compound (Hd) is F. In another embodiment, Y in compound (lid) is I. In another S embodiment," Q in compound (lId) is NHCOCHs. In-another embodiment, Q in Ci compound (lid) is F. In another embodiment, Q in compound (lid) is Cl. In another embodiment, Q in compound (Hd) is Br. In another embodiment, Q in compound (Hd) is I. In another embodiment, T in compound (Hd) is OH. In another embodiment, RI in compound (lid) is CH 3 [000108] The substituents Z and Y can be in any position of the ring carrying these substituents (hereinafter "A ring"). In one embodiment, the substituent Z is in the para position of the A ring. In another embodiment, the substituent Y is in the meta position of the A ring. In another embodiment, the substituent Z is in the para position of the A ring and substituent Y is in the meta position of the A ring. In another embodiment, the substitutent Z is NO 2 aid is in the para position of the A ring. In another embodiment, the substitutent Zis CN and is in the paraposition of the A ring. In another embodiment the substitutent Z is F and is in the para position of the A ring. In another embodiment, the substitutent Z is Cl and is in the pam position of the A ring. In another embodiment, the substitutent Z is Br and is in the para position of the A ring. In another embodiment the substitutent Z is I and is in the para position of the A ring. In another embodiment, the substitutent Z is H. In another embodiment, the substitutent Y is CF 3 and is in the meta position of the A ring. In another embodiment, the substitutent Y is F and is in the meta position of the A ring. In another embodiment, the substitutent Yis Cl and is in the meta position of the A ring. In another embodiment the substitutent Y is Br and is in the D meta position of the A ring. In another embodiment, the substitutent Y is I and is in the o meta position of the A ring.

,0 [000109 In another embodiment, one of Zor Y together with the benzene ring to m which it is attached is a fused bicyclic carbocyclic or heterocyclic ring system, such as, but not being limited to: natphthyl,'quinalzolyl, pyrimidinyl, and the like..

S [000110] The substituent :Q can be in any position of the ring carrying these o 10 substituents (hereinafter "B ring"). In one embodiment, the substitutent Q is in the para N position of the B ring. In another embodiment, Q is F and is in the para position of the B S ring. In another embodiment, Q is Cl and is in the para position of the B ring. In another embodiment, Q is Br and is in the para position of the B ring. In another embodiment, Q is I and is in the para position of the B.ring. In another embodiment .Q is NHCOCH 3 and is in the para position of the B ring.

t000111] The substituent R is defined herein as an alkyl, haloalkyl, dihaloalkyl, trihaloalkyl, CH 2 F, CHF2, CF 3

CF

2

CF

3 aryl, phenyl, F, CI Br, I, alkenyl, or hydroxyl

(OH).

[000112] An "alkyl" group refers to a saturated aliphatic hydrocarbon, including straightchain, branched-chain and cyclic alkyl groups. In one embodiment, the alkyl group has 1-12 carbons. In another embodiment, the alkyl group has 1-7 carbons. In another embodiment, the alkyl group has 1-6 carbons. In another embodiment, the alkyl group has 1-4 carbons. The alkyl group may be.unsubstituted or substituted by one or more groups selected from halogen F, Cl, Br, hydroxy, alkoxy carbonyl, amido, alkylamido, dialkylamido, nitro, amino, alkylamino, dialkylamin, carboxyl, thio and thioalkyl.

[000113] A "haloalkyl" group refers to an alkyl group as defined above, which is substituted by one or more halogen atoms, e.g. by F, Cl, Br or I. A "halogen" refers to elements of Group VII or the periodic table, e.g. F, Cl, Br or I.

[000114] An "aryl" group refers to an aromatic group having at least one carbocyclic O aromatic group or heterocyclic aromatic group, which may be 'unsubstituted or t C substituted by one or more groups selected from halogen F, Cl, Br, haloalkyl, 5 hydroxy, alkoxy carbonyl, amido, alkylamido, dialkylamido, nitro, amino, alkylamino, Cc dialkylamino, carboxy or thio or thioalkyl. Nonlimiting examples of aryl rings are

C

phenyl, naphthyl, pyranyl, pyrrolyl, pyrazinyl, pyrimidinyl, pyrazolyl, pyridinyl, furanyl, thiophenyl, thiazolyl, imidazolyl, isoxazolyl, and the like.

[000115] A "hydroxyl" group refers to an OH group. An "alkenyl" group refers to a group having at least one carbon to carbon double bond.

L

C

[000116] An "arylalkyl" group refers to.an alkyl bound to an aryl, wherein alklyl and aryl are as defined above.. An example of an aralkyl group is a benzyl group.

[000117] As contemplated herein, the present invention relates to the use of a SARM compound and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, "pharmaceutical product, hydrate, N-oxide, prodrug, polymorph or crystal or combinations thereof. In one embodiment, the invention relates to the use of an analog of the SARM compound. In another embodiment, the invention relates to the use of a derivative of the SARM compound. In another embodiment, the invention relates to the use of an isomer of the SARM compound. In another embodiment, the invention relates to the use of a metabolite of the SARM compound. In another embodiment, the invention relates to the use of a pharmaceutically acceptable salt of the SARM compound. In another embodiment, the invention relates to the use of a pharmaceutical product of the SARM compound. In another embodiment, the invention relates to the use of a hydrate of the SARM compound. In another embodiment, the invention relates to the use of an N-oxide of the SARM compound. In another embodiment, the invention, relates to the use of a prodrug of the SARM compound. In another embodiment, the invention relates to the use of a polymorph of the SARM compound. In another embodiment, the invention relates to the use of a crystal of the SARM compound. In another embodiment, the invention relates to the use of any of a combination of an analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, or N-oxide, prodrg, polymorph or crystal of the SARM compounds of O the present invention a) 5 [000118] As defined herein, the taem "isomer" includes, but is. not limited to, optical isomers and analogs, structural isomers and analogs, conformational isomers and

C

l analogs, and the like....

1 [000119] In one embodiment, this invention encompasses the use of various optical r- 0 10 isomers of the SARM compounds. It will be appreciated by those skilled in the art that c the SARM compounds of the present invention contain at least one chiral center.

o Accordingly, the SARM compounds used in the methods of the present invention may C exist in, and be isolated in, optically-active or racemic forms. Some compounds may also exhibit polymorphism. It is to be understood that the .present invention encompasses any racemic, optically-active, polymorphic, or stereroisomeric form, or mixtures thereof, which form possesses properties useful in the methods as described herein. In one embodiment, the SARM compounds are the pure (R)-isomers. In another embodiment, the SARM compounds are the pure (S)-isomers. In another embodiment, the SARM compounds are a mixture of the and the isomers. In another 0o embodiment, the SARM compounds are a racemic mixture comprising an equal amount of the and the isomers. It is well known in the art how to prepare optically-active forms (for example, by resolution of the racemic form by recrystallization techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary phase).

[000120] The invention includes pharmaceutically acceptable salts of amino-substituted compounds with organic and inorganic acids, for example, citric acid and hydrochloric acid. The invention also includes N-oxides of the amino substituents of the compounds described herein. Pharmaceutically acceptable salts can also be prepared from the phenolic compounds by treatment with inorganic bases, for example, sodium hydroxide.

Also, esters of the phenolic compounds can be made with aliphatic and aromatic carboxylic acids, for example, acetic acidand benzoic acid esters.

[000121] This invention further includes derivatives of the SARM compounds. The tenn 0 "derivatives" includes but is not limited to ether: derivatives, acid derivatives, amide Sderivatives, ester derivatives and the like. In addition, this invention further includes S5 hydrates of the SARM compounds. The term "hydrate" includes but is not limited to hemihydrate, monohydrate, dihydrate, trihydrate and the like. ci [000122] This invention further includes metabolites of the SARM compounds. the term "metabolite" means any substance produced from another substance by metabolism or a 0 10 metabolic process.

ID

O [000123] This invention further includes pharmaceutical products of the SARM 0 Ci compounds. The term "pharmaceutical product" means a composition suitable for pharmaceutical use (phannaceutical composition), as defned herein.

-[000124] This invention further includes prodrugs of the SARM compounds. The term "prodrug" means a substance which can be converted in-vivo into a biologically active agent by such reactions as hydrolysis, esterification, desterification, activation, salt formation and the like.

[000125] This invention further includes crystals of the SARM compounds.

Furthermore, this invention provides polymorphs of the SARM compounds. The term "crystal" means a substance in a crystalline state. The term "polymorph" refers to a particular crystalline state of a substance, having particular physical properties such as X-ray diffraction, IR spectra, melting point, and the like.

BIOLOGICAL ACTIVITY OF SELECTIVE ANDROGEN RECEPTOR MODULATOR COMPOUNDS [0001261 Selective androgen receptor modulator (SARM) compounds are a novel class of androgen receptor targeting agents that have previously been shown to be useful for a) male contraception; b) treatment of a variety of hormone-related conditions, for example conditions associated with Androgen Decline in Aging Male (ADAM), such as fatigue, depression, decreased libido, sexual dysfunction, erectile dysfunction, hypogonadism, osteoporosis; hair loss,. anemia, obesity, sarcopenia, osteopenia, osteoporosis, benign prostate hyperplasia, alterations in mood and cognition and prostate cancer; c) treatment .of conditions associated with Androgen Decline in Female (ADIF), such as sexual dysfunction, decreased sexual libido, hypogonadism, sarcopenia, osteopenia, osteoporosis, alterations in cognition and mood, depression, anemia, hlir loss, obesity, endometriosis, breast cancer, uterine cancer and ovarian cancer; d) treatment and/or prevention of acute and/or chronic muscular wasting conditions; e) preventing and/or treating dry eye conditions; f) oral androgen replacement therapy; g) decreasing the incidence of halting or causing a regression of prostate cancer; and/or ducing apoptosis in a cancer cell.

1000127] As used herein, .receptors for. extracellular signaling molecules are collectively referred to as "cell signaling receptors". Many cell signaling receptors are transmembrane proteins on a'cell surface; when. they bind an extracellular signaling molecule a ligand), they become activated so as to generate a cascade of intracellular signals that alter the behavior of the celL In contrast, in some cases, the receptors are inside the cell and the signaling ligand has to enter the cell to activate them; these signaling molecules therefore must bie sufficiently small and hydrophobic to diffuse across the plasma membrane of the cell.

[000128] Steroid hormones are one example of small hydrophobic molecules that diffuse directly across the plasma membrane of target cells and bind to intracellular cell signaling receptors. These receptors are structurally related and constitute the intracellular receptor superfamily (or steroid-hormone receptor superfamily). Steroid honnone receptors include progesterone receptors, estrogen receptors, androgen receptors, glueocorticoid receptors, and mineralocorticoid receptors. The: present invention is particularly directed to androgen receptors.

[000129] In addition to ligand binding to the receptors, the receptors can be blocked to prevent ligand binding. When a substance binds to a receptor, the threedimensional structure of the substance fits into a space created by the three-dimensional structure of the receptor in a ball and socket configuration The better the ball fits into o the socket the more tightly it is held. This phenomenon is called affinity. If the affinity of a substance is greater than theoriginal hormone, it will compete with the hormone 9 5 and bind the binding site more frequently. Once'bound, signals may be sent through the receptor into the cell, causing the cell to respond in some fashion. This is called Clq activation. On activation, the activated receptor then directly regulates the transcription of specific. genes. But the substance and the receptor may have certain attributes, other than affinity, in order to activate the cell. 'Chemical -bonds between atoms of the o 10 substance and the atoms of the receptors may form., In some cases,'this leads to a change Cl in the configuration of the receptor, which is, enough to, begin the activation process o (called signal transduction). [000130] In one embodiment the present invention is directed to selective androgen receptor modulator compounds which are agonist compouhds. A receptor agonist is a substane which binds receptors and activates them. Thus, in one embodiment, the SARM compounds of the present invention ar useful in binding toand activating steroidal hormone receptors. In one embodiment, the agonist compound of the present invention is an agonist which binds the androgen receptor. In another embodiment, the compound has high affinity for the androgen receptor. In another embodiment the agonist compound also has anabolic activity. In another embodiment, the present invention provides selective androgen modulator compounds which have agonistic and anabolic activity of a nonsteroidal compound for the androgen receptor.

[000131) In another embodiment, the present invention is directed -to selective androgen receptor modulator compounds which are antagonist compounds. A receptor antagonist is a substance which binds receptors and inactivates them. Thus, in one embodiment, the SARM compounds of the present invention are useful in binding to and inactivating steroidal hormone receptois. In one embodiment, the antagonist compound of the present invention is an antagonist which binds the androgen receptor. In another embodiment, the compound has high affinity for the androgen receptor.

[000132) In yet another embodiment, the SARM compounds of the present invention can be classified as partial AR agonist/antagonists. The SARMs are AR agonists in some tissues, to cause increased transcription of AR-responsive genes (e.g.

muscle anabolic effect). In other tissues, these compounds serve as inhibitors at the AR to prevent agonistic effects of the native androgens. i [000133] Assays to determine whether the compounds of the present invention are AR agonists or antagonists are well known to a person skilled in the art. For example, AR agonistic activity can be determined by monitoring the ability of the" SARM compounds to maintain and/or stimulate the growth of AR containing tissue such as prostate and seminal vesicles, as measured by weight. AR antagonistic activity can be determined by monitoring the ability of the SARM compounds to inhibit the growth of AR containing tissue.

[000134] The compounds of the present invention, bind either reversibly or irreversibly to an androgen receptor. In one embodiment, the androgen receptor is an androgen receptor of a mammal. In another embodiment, the androgen receptor is an androgen receptor of a human. In one embodiment, the SARM compounds bind reversibly to the androgen receptor of a mammal, for example a human. Reversible binding of a compound to a. receptor means that a compound can detach from the receptor after binding. [000135] In another embodiment, the SARM compounds bind irreversibly to the androgen receptor of a mammal, for example a human. Thus, in one embodiment, the compounds of the present invention may contain a functional group affinity label) that allows alkylation of the androgen receptor covalent bond formation). Thus, in this case, the compounds are alkylating agents which bind irreversibly to the receptor and, accordingly, cannot be displaced by a steroid, such as the endogenous ligands DHT and testosterone. An "alkylating agent' is defined herein as an agent which alkylates (forms a covalent bond) with a cellular component such as DNA, RNA or enzyme. It is a highly reactive chemical that introduces -alkyI radicals -into biologically active molecules and thereby prevents their proper functioning. The alkylating moiety is an electrbphilic group that interacts with nucleophilic moieties in cellular components.

[000136] According to one embodiment of the present invention, a method is provided for binding the SARM compounds of the present invention to an androgen receptor by contacting the receptor with a SARM compound and/or its analog, derivative, isomer, metabolite, phannaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination. thereot under conditions effective to cause the selective androgen receptor modulator compound to bind the androgen receptor. The binding of the selective androgen receptor modulator S compounds to the androgen receptor enables the compounds of the present invention to o be useful as a male contraceptive and in a number of hormone therapies. The agonist compounds bind to and activate the androgen receptor. The antagonist compounds bind to and inactivate the androgen receptor. Binding of the agonist, or antagonist compounds is either reversible or irreversible.

[000137] According to one embodiment of the present invention, a method is provided for suppressing spermatogenesis in a subject by contacting an androgen receptor of the subject with a SARM compound of the present invention and/or -its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to bind the selective androgen receptor modulator compound to the androgen receptor and suppress spermatogenesis.

[000138] In another embodiment, the present invention provides amethod of contraception in a male subject, comprising the step of administering to the subject a SARM compound of the present invention, and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to suppress sperm production in the subject, thereby effecting contraception in the subject [000139] According to another embodiment of the present invention, a method is provided for hormonal therapy in a patient one suffering from an androgen- O dependent condition) which includes contacting an androgen receptor of a patient with a C SARM compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, phanmaceutical product, hydrate, N-oxide, c prodrug, polymorph, crystal or any combination thereof, in an amount effective to bind the selective androgen receptor modulator compound to the androgen receptor and effect a change in an androgen-dependent condition.

[000140] According to another embodiment of the present invention, a method is provided for hormonal replacement therapy in a patient which includes contacting an S androgen receptor of a patient with a SARM compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to bind the selective androgen receptor modulator compound to the androgen receptor and effect a change in an androgendependent condition.

[000141] According to another embodiment of the present invention, a method is provided for treating a .subject having a hormone related condition which includes administering to the subject a SARM compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to bind the SARM compound to the androgen receptor and effect a change in an androgen-dependent condition.

[000142] Androgen-dependent conditions which may be treated according to the present invention include those conditions which are associated with aging, such as hypogonadism, sarcopenia, erythropoiesis, osteoporosis, and any other conditions determined to be dependent upon low androgen testosterone) levels.

[000143] According to another embodiment of the present invention, a method is provided for treating a subject suffering from prostate cancet, comprising the step of S administering to the subject a SARM compound of the present invention and/or its I analog, derivative, isomer, metabolite, pharnmaceutically acceptable salt, phanmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to treat prostate cancer in the subject [000144] According to another embodiment of the present invention, a method is provided for preventing prostate cancer in a subject, comprising the step of administering to the subject a SARM compound of the present invention and/or its I analog, derivative, isomer, metabolite, pharmaceutically acceptable salt pharmaceutical S product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an I amount.effective to prevent prostate cancer in the subject..

[000145] According to another embodiment of the present invention, a method is provided for delaying the progression of prostate cancer in a subject suffering from prostate cancer, comprising the step of administering to the subject a SARM compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, .0 polymorph, crystal or any combination thereof, in an amount effective to delay the progression of prostate cancer in the subject.: [000146] According to another embodiment of the present invention, a method is provided for preventing the recurrence of prostate cancer in a subject suffering from prostate cancer, comprising the step of administerg to the subject a SARM compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to prevent the recurrence of prostate cancer in the subject.

[000147] According to another embodiment of the present invention, a method is provided for treating the recurrence of prostate cancer in a subject suffering from prostate cancer, comprising the step of administering to the subject a SARM compound of the present invention and/or its analog, derivative, isomer, metabolite, o pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, CN polymorph, crystal or any combination thereof in an amount effective to treat the 5 recurrence of prostate cancer in the subject C [000148] Furthermore, stimulation of the Androgen Receptor stimulates the production of tears, and thus the SARM compounds of the present invention may be used to treat dry eye conditions. .Therefore, according to another embodiment of the present invention, a method is provided for treating a dry eye condition in a subject C suffering from dry eyes, comprising the step of administering to -said subject the o selective androgen receptor modulator compound of the present invention and/or its Cl analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereo in an amount effective to treat dry eyes in the subject.

[000149] According to another embodiment of the present invention, a method is provided for preventing a dry eye condition in a subject, comprising the step of administering to said subject the selective androgen receptor modulator compound of the present invention and/or its. analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof in an amount effective to prevent dry eyes in the subject [000150] In another embodiment, the present invention provides a a method of inducing apoptosis in a cancer cell, comprising the step of contacting the cell with with the selective androgen receptor modulator compound of the present invention and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal or any combination thereof, in an amount effective to induce apoptosis in said cancer cell.

[000151] As defined herein, "contacting" means that the SARM compound of the present invention is introduced into a sample containing the enzyme in a test tube, flask, o tissue cultureor.Chip, array, plate, microplate, capillary, or the like, and incubated at a N' 'temperature and time sufficient to permit binding of the SARM to the enzyme. -Methods C) 5 for. contacting the samples with the SARM or other specific binding components are .known to those skilled in the art and may be selected depending on the type of assay protocol to be run. Incubation methods are also standard and are known to those skilled in the art. [000152] In another embodiment, the term "contacting" means that the SARM compound of the present invention is introduced into a subject receiving treatment and Sthe SARM compound is allowed to come in contact with.the androgen receptor min vivo.

[000153] The term 'libido,. as used herein, means sexual desire. :000154] The term "erectile", as used herein, means capable of being erected. An erektile tissue is a tissue, which is capable of being greatly dilated and made rigid by the distension of the numerous blood vessels which it contains.

[000155] "Hypogonadism" is a condition resulting from or characterised bj abnormally decreased functional activity of the gonads, with retardation of growth and sexual development "Osteopenia" refers to decreased calcification or density of bone.

This is a term which encompasses all skeletal systems in which such a condition is noted.. [000156] "Osteoporosis" refers to a thinning of the bones with reduction in bone mass due to depletion of calcium and bone protein. Osteoporosis predisposes a person to fractures, which are often slow to heal and heal poorly. Unchecked osteoporosis can lead to changes in posture, physical abnormality, and decreased mobility.

[000157] "BPH (benign prostate hyperplasia)" is a nonmalignant enlargement of the prostate gland, and is the most common non-malignant proliferative abnormality found in any internal organ and the major cause of morbidity in the adult male. BPH IN occurs in over 75% of men over 50 years of age, reaching 88% prevalence by the ninth 0 S decade. BPH frequently results in a-gradual squeezing of the portion of the urethra which traverses the prostate (prostatic urethra). This causes patients to experience a frequent urge to urinate because ofincomplete emptying of the bladder and urgency of cn urination. The obstruction of urinary flow can also lead to a general lack of control over C<1 urination, including difficulty initiating urination when desired, as well as difficulty in preventing urinary flow because of the inability to empty urine from the bladder, a condition known as overflow urinary incontinence, which can lead to urinary obstruction o 10 and to urinary failure.

IN

0 S [000158] "Cognition"-refers to the process of knowing, specifically the process of C'q being aware, knowing, thinking, learning and judging. Cognition is related to the fields o.f psychology, linguistics, computer science, neuroscience, mathematics, ethology and philosophy. The term "mood" refers to a temper or state of the mind. As contemplated herein, alterations means any change for the positive or- negative, in cognition and/or m ood. [000159] The term "depression" refers to an illness that involves the body, mood and thoughts, that affects the way a person eats, sleeps and the way one feels about oneself, and thinks about things. The signs and symptoms of depression include loss of interest in activities, loss of appetite or overeating, loss of emotional expression, an empty mood, feelings of hopelessness, pessimism, guilt or helplessness, social withdrawal, fatigue, sleep disturbances, trouble concentrating, remembering, or making decisions, restlessness, irritability, headaches, digestive disorders or chronic pain.

[000160] The term "hair loss", medically known as alopecia, refers to baldness as in the very common type of male-pattern baldness. Baldness typically begins with patch hair loss on the scalp and sometimes progresses to complete baldness and even loss of body hair. Hair loss affects both males and females.

[000161] "Anemia" refers to the condition of having less than the normal number IN of red blood cells. or less than the normal quantity of hemoglobin in the blood. The o oxygen-carrying capacity of the blood is, therefore, decreased. Persons with anemia may feel tired and fatigue easily, appear pale, develop palpitations and become usually .5 short of breath. 'Anemia is caused by. four basic factors: a) hemorrhage (bleeding); b) S hemolysis (excessive destruction of red blood cells); c) underproductin 'of red blood cells; and d) not enough normal .hemoglobin. There are many forms of anemia, including aplastic anemia, benzene poisoning, Fanconi anemia, hemolytic disease of the newborn, hereditary spherocytosis, iron deficiency anemia, osteopetrosis, pernicious o 10 anemia, sickle. cell disease, thalassemia, myelodysplastic syndrome, and a variety of N bone marrow diseases. As contemplated herein, the SARM compounds of the present O invention are useful in preventing and/or treating any one or more of the above-listed forms of anemia. [000162] "Qbesity" refers to the state of being well above one's normal weight.

Traditionally, a.person is considered to be obese if they are more than 20 percent over their ideal weight. Obesity has been more precisely defined by the National Institute of Health (NIBT) as a Body to Mass Index (BMI) of 30 or above. Obesity is 'often multifactorial, based on both genetic and behavioral factors. Overweight due to obesity is a significant contributor to health problems. It increases the risk of developing a number of diseases including: Type 2 (adult-onset) diabetes; high blood pressure (hypertension); stroke (cerebrovascular accident or CVA); heart attack .(myocardial infarction or MI); heart failure (congestive heart failure); cancer (certain forms such as cancer of the prostate and cancer of the colon and rectum); gallstones-and gallbladder.

disease (cholecystitis); Gout and gouty arthritis; osteoarthritis (degenerative arthritis) of the knees, hips, and the lower back; sleep apnea (failure to breath normally during sleep, lowering blood oxygen); and Pickwickian syndrome (obesity, red face, underventilation and drowsiness). As contemplated herein, the term "obesity" includes any one of the above-listed obesity-related conditions and diseases. Thus the SARM compounds of the present invention are useful in preventing and/or treating obesity and any one or more of the above-listed obesity-related conditions and diseases.

[000163] "Prostate cancer" is one of the most frequently occurring cancers among I0 men in the United States, with hundreds of thousands of new cases diagnosed each year.

o Over sixty percent of newly diagnosed cases of prostate cancer are found to be pathologically advanced, with no cure and a dismal prognosis. One third of all men over 4 5 50 years of age have a latent form of prostate cancer that may be activated into the lifethreatening clinical prostate cancer form. The frequency of latent prostatic tumors has been shown to increase substantially with each decade of life from the 50s to 0C the 90s The number of people with latent prostate cancer is the same across all cultures, ethnic groups, and races, yet the frequency of clinically aggressive cancer is 0 to markedly different. This suggests that environmental factors may play a role in IND activating latent prostate cancer., PHARMACEUTICAL COMPOSITIONS [000164] The treatment methods of. the -present invention comprise, in one embodiment, administering a phannmaceutical preparation comprising the SARM compound and/or its analog, :derivative, isomer, metabolite, parmaceutically acceptable salt, pharmaceutical product, hydrate, N-oxide, prodrug, polymorph, crystal 'or any combination thereof; and a pharmaceutically acceptable carrier.

[000165] As used herein, "pharmaceutical composition" means a composition comprising an "effective amount" of the active ingredient, i.e. the SARM compound, together with a pharmaceutically acceptable carrier or diluent.

[0001661 An "effective amount" as used herein refers to that amount which provides a therapeutic effect for a given condition and administration regimen. An "effective amount" of the SARM compounds as used herein can be in the range of 1-500 mg/day. In one embodiment the dosage is in the range of 1-100 mg/day. In another embodiment the dosage is in the range of 100-500 mg/day. In another embodiment the dosage is in a range of 45-60 mg/day. In another embodiment the dosage is in the range of 15-25 mg/day. In another embodiment the dosage is in the range of 55-65 mg/day. In another embodiment the dosage is in the range of 45-60 mg/day. The SARM compounds can be administered daily, in single dosage forms containing the entire IN amount of daily dose, or can be administered daily in multiple doses such as twice daily 0 0 or three times daily. The SARM. compounds can also be administered intemittently, for example every other day, 3 days a week, four days a week, five days a week and the 5 -lik e. [000167] As used herein, the term "treating" includes preventative as well as disorder rermitative treatment. ",As used herein, the terms "reducing", "suppressing" and S "inhibiting" have their commonly understood meaning of lessening or decreasing. As 0 0 10 used herein, the term 'Tacilitating" is giving its commonly understood meaning of IND increasing the rate. As used herein, the term "promoting" is given its commonly 0 0 understood meaning of increasing. As used herein, the term "progression". means increasing in scope or severity, advancing, growing or becoming worse. [000168] As used herein, the term "administering" refers to bringing a subject in contact with a SARM compound of the present invention. As used herein, administration can be accomplished in vitro, i.e. in a test tube, or in vivo, i.e. in cells or tissues of living organisms, for example humans. In one embodiment, the present invention encompasses administering the compounds of the present invention to a subject. In one embodiment, the subject is a mammalian subject .In another embodiment the subject is a human.

[000169] The pharmaceutical compositions containing the SARM agent can be administered to a subject by any method known to a person skilled in the art, such as parenterally, paracancerally, transmucosally, transdemmally, intramuscularly, intravenously, intradermally, subcutaneously, intraperitonealy, intraventricularly, intracranially, intravaginally or intratumorally.

[000170] In one embodiment, the pharmaceutical compositions are administered orally, and are thus formulated in a form suitable for oral administration, i.e. as a solid or a liquid preparation. Suitable solid oral formulations include tablets, capsules, pills, granules, pellets and the like. Suitable liquid oral formulations include solutions, suspensions, dispersions, emulstions, oils and the like. In one embodiment of the present invention, the SARM compounds are formulated in a capsule. In accordance with this embodiment, the,compositions of the present invention comprise in addition to the SARM active compound and the inert carrier or diluent, a hard gelting capsule.

[000171] Further, in another embodiment, the pharmaceutical compositions are administered by intravenous, intraarterial, or intramuscular injection of a liquid preparation. Suitable liquid formulations include solutions, suspensions, dispersions, emulsions, oils and the like. In one embodiment, the pharmaceutical compositions are administered intravenously, and are thus formulated in a form suitable for intravenous administration. In another, embodiment, the pharmaceutical compositions are administered intraarterially, and -are thus formulated in a form suitable for intraarterial administration. In another, embodiment, the -pharmaceutical compositions are administered intramuscularly, and are thus .formulated .in a form suitable for intramuscular administration.

[000172] Further, in another embodiment, the pharmaceutical compositions are administered topically to body surfaces, and are thus formulated in a form suitable for topical, administration. Suitable .topical formulations include gels, ointments, creams, lotions, drops and the like.. For topical administration, the SARM agents or their o physiologically tolerated derivatives such as salts, esters, N-oxides, and the like are prepared and applied as solutions, suspensions, or emulsions in a physiologically acceptable.diluent with or without a pharmaceutical carrier. [000173] Further, in another embodiment, the pharmaceutical compositions are administered as a suppository, for example a rectal suppository or a urethral suppository.

Further, in another embodiment, the pharmaceutical compositions are administered by subcutaneous implantation of a pellet. In a further embodiment, the pellet provides for controlled release of SARM agent over a period of time.

[000174] In another embodiment, the active compound can be delivered in a vesicle, in particular a liposome (see Langer, Science 249:1527-1533 (1990); Treat et aL, in Liposomes in the Therapy of Infectious Disease. and Cancer, Lopez- Berestein and Fidler Liss, New York, pp. 353-365 (1989); Lopez-Berestein, ibid., pp. 317-327; \D see generally ibid).

O

[000175] As used herein "pharmaceutically acceptable carriers or diluents" are well known to those skilled in the art The carrier ordiluent may be a solid carrer or diluent Cc for solid formuations, a liquid carrier or diluent for liquid formulations, ;or,mixtures thereof r [000176] Solid carriers/diluents include, but are not limited to, a gum, a starch (e.g.

0 I corn starch, pregeletanized starch), a sugar lactose, mannitol, sucrose, dextrose), a 0 cellulbsic material' microcrystalline cellulose), an -acrylate (e.g.

0 O polymethylacrylate), calcium carbonate, magnesium oxide, talc, or mixtures thereof.

10001771] For, liquid formulations, phamnaceutically acceptable carriers may be aqueous or non-aqueous solutions, suspensions, emulsions or oils. Examples of non- •aqueous solvents are propylene glycol, polyethylene glycol, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous, solutions, emulsions or suspensions, including saline and.buffered.media. Examples of oils are those of petroleum, animal, vegetable, or synthetic origin, for example,, peanut. oil, soybean oil, mineral oil, olive oil, sunflower oil, and fish-liver oil.

[000178] Parenteral vehicles (for subcutaneous, intravenous, intraarterial, or intramuscular injection) include sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's and fixed oils. Intravenous vehicles include-fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose, and the like. Examples are sterile liquids such as water and oils, with or without the addition of a surfactant and other pharmaceutically acceptable adjuvants. In general, water, saline, aqueous dextrose and related sugar solutions, and glycols such as propylene glycols or polyethylene glycol are preferred liquid carriers, particularly for injectable solutions.. Examples of oils are those of petroleum, animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, mineral oil, olive oil, sunflower oil, and fish-liver oil..

N0 [000179] In addition, the compositions may further comprise binders acacia, 0 0 cornstarch, gelatin, carbomer, ethyl cellulose, guar gum, hydroxypropyl cellulose, ciq hydroxypropyl methyl cellulose, povidone), disintegrating agents cornstarch, potato

C)

starch, alginic acid, silicon dioxide, croscarmelose sodium, crospovidone, guar gum, c sodium starch glycolate), buffers Tris-HCL, acetate, phosphate) of various pH and ionic strength, additives such as albumin or gelatin to prevent absorption to surfaces, 0C detergents Tween 20, Tween 80, Pluronic F68, bile acid salts), protease inhibitors, surfactants sodium lauryl sulfate), permeation enhancers, solubilizing agents 0 O to glycerol, polyethylene glycerol), anti-oxidants ascorbic acid, sodium metabisulfite, IN butylated hydroxyanisole), stabilizers hydroxypropyl cellulose, 0 hyroxypropylmethyl cellulose), viscosity increasing agents(e.g. carbomer, colloidal silicon dioxide, ethyl cellulose, guar gum), sweetners aspartame, citric acid), prservatives Thimerosa l benzyl alcoho,,parabens), lubricants stearic acid, magnesium stearate, polyethylene.glycol, sodium lauryl sulfate), flow-aids colloidal silicon dioxide), plasticizers .diethyl phthalate, triethyl citrate), emulsifiers (e.g.

carbomer, hydroxypropyl cellulose, sodium lauryl sulfate), polymer' coatings poloxamers or poloxamines), coating and film forming agents. ethyl cellulose, acrylates, polymethacrylates) and/or adjuvants.

[000180] In one embodiment, the pharmaceutical compositions provided herein are controlled release compositions, i.e. compositions in which the SARM compound is released over a period of time after administration. Controlled or.sustained release compositions include formulation in lipophilic depots-(e.g. fatty acids, waxes, oils). in another embodiment, the composition is an immediate release composition, i.e. a composition in which all of the SARM compound is released immediately after administration [000181] In yet another embodiment, the pharmaceutical composition can be delivered in a controlled release system. For example, the agent may be administered using intravenous infusion, an implantable osmotic pump, a transdermal patch, liposomes, or other modes of administration. In one embodiment, a pump may be used (see Langer, supra; Sefton, CRC Crit. Ref. Biomed. Eng. 14:201 (1987); Buchwald et S al., Surgery 88:507 (1980); Saudek et al., N. Engl. J. Med. 321:574 (1989). In another

O

o embodiment, polymeric materials can be used. In yet another embodiment, a controlled release system can be placed in proximity to the therapeutic target, the brain, thus requiring only a fraction of the systemic dose (see, Goodson, in Medical cn Applications of Controlled Release, supra, vol. 2, pp. 115-138 (1984). Other controlled release systems are discussed in the review by Langer (Science 249:1527-1533 (1990).

[000182] The compositions may also include incorporation of the active material 0 0 10 into or onto particulate preparations of polymeric compounds such as polylactic acid, N0 polglycolic acid, hydrogels, .etc, or onto liposomes, microemulsions, micelles, 0 0 unilamellar or multilamellar vesicles, erythrocyte ghosts, or spheroplasts.) Such compositions will influence the physical state, solubility, stability, rate of in vivo release, .and rate.of.in vivo clearance.

[000183] Also comprehended by the invention are particulate compositions coated .with polymers .poloxamers or poloxamines) and the compound coupled to antibodies directed against tissue-specific receptors, ligands or antigens or coupled to ligands of tissue-specific receptors.

[900184] Also comprehended by the invention are compounds modified by the covalent attachment of water-soluble polymers such as polyethylene glycol, copolymers of polyethylene glycol and polypropylene glycol, carboxymethyl cellulose,. dextran, polyvinyl alcohol;-, polyvinylpyrrolidone or polyproline. The modified compounds are lknown to exhibit substantially longer half-lives in blood following intravenous injection than do the corresponding unmodified compounds (Abuchowsld et al., 1981; Newmark et al., 1982; and Katre et al., 1987). Such modifications may also increase the compound's solubility in aqueous solution, eliminate aggregation, enhance the physical and chemical stability of the compound, and greatly reduce the immunogenicity and reactivity of the compound. As a result, the desired in vivo biological activity may be achieved by the administration of such polymer-compound abducts less frequently or in lower doses than with the unmodified compound.

IN [000185] The preparation of pharmaceutical compositions which contain an active O component is well understood in the art, for example by mixing, granulating, or tablet-

C)

forming processes. The active therapeutic ingredient is often mixed with excipients which are pharmaceutically acceptable and compatible with the active ingredient For eC oral administration, the SARM agents or their physiologically tolerated derivatives such as salts, esters, N-oxides, and the like are mixed with additives customary for this 0 purpose, such as vehicles, stabilizers, or inert diluents, and converted by customary methods into suitable forms for administration, such as tablets, coated tablets, hard or 0 S 10 soft gelatin capsules, aqueous, alcoholic or oily solutions. For parenteral administration, the. SARM agents or their physiologically tolerated derivatives such as salts, esters, N-

O

O oxides, and the like are converted into a solution, suspension, or emulsion, if desired with the substances customary and suitable for this purpose, for example, solubilizers or .other.

[000186] An active component can be formulated into the composition as neutralized pharmaceutically acceptable salt forms. Pharmaceutically acceptable salts include the acid addition salts (formed with the free amino groups of the polypeptide or antibody, molecule), which are formed with inorganic acids such as, for example, -hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like. Salts formed from the free carboxyl groups can also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, .2ethylamino ethanol, histidine, procaine, and the like.

[000187] For use in medicine, the salts of the SARM will be pharmaceutically acceptable salts. Other salts may, however, be useful in the preparation of the compounds according to the invention or of their phannaceutically acceptable salts.

Suitable pharmaceutically acceptable. salts of the compounds of this invention include acid addition salts which may, for example, be formed by mixing a solution of the compound according to the invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulphuric acid, methanesulphonic acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic: acid, oxalic acid, citric acid, tartaric acid, I0 carbonic acid or phosphoric acid.

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[0001881 In one embodiment, the methods of the present invention comprise administering a SARM -compound as the sole active' ingredient t However, also encompassed within the scope of the present invention are methods of a) male contraception; b) treatment of a variety of hormone-related conditions, for example conditions associated with Androgen Decline in Aging Male (ADAM); c) treatment of conditions associated with Androgen Decline in Female (ADIP); d) treatment and/or prevention of acute and/or chronic muscular wasting conditions; e) preventing and/or Streating dry eye conditions; f) oral androgen replacement therapy; g) decreasing the 0 incidence ot halting or causing a regression of prostate cancer; andr h) inducing apoptosis in a cancer cell as disclosedhefrein, which comprise administering the SARM compounds in combination.with one or more therapeutic agents. .These agents include,.

but are not limited to: LHRH analogs, reversible antiandrbgens, antiestrogens, anticancer drugs, 5-alpha reductase inhibitors, aromatase inhibitors, progestins, or agents acting through other nuclear hormone receptors.

[0001891 Thus, in one embodiment, the present invention provides compositions and pharmaceutical compositions comprising a selective androgen receptor modulator compound, in combination with an LHRH analog. In another embodiment, the present invention provides 'compositions and pharmaceutical compositions comprising a selective androgen receptor modulator compound, in combination with a reversible antiandrogen. In another embodiment;-the present invention provides compositions and pharmaceutical compositions comprising a selective androgen receptor modulator compound, in combination with an antiestrogen. In another embodiment, the present invention provides compositions and pharmaceutical .compositions comprising a selective androgen receptor modulator compound, in combination with an anticancer drug. In another embodiment, the present invention provides compositions and pharmaceutical compositions comprising a selective androgen receptor modulator compound, in combination with a 5-alpha reductase inhibitor. In another embodiment, the present invention provides compositions and pharmaceutical compositions comprising a selective androgen receptor modulator compound, in combination with an IN aromatase inhibitor. In another embodiment, the present invention provides compositions and pharmaceutical compositions comprising a selective androgen receptor modulator compound, in combination with a progestin. In another embodiment, the present invention provides compositions and pharmaceutical compositions comprising a Cen selective androgen receptor modulator compound, in combination with an agent acting through other nuclear hormone receptors.

O 10.

N

[000190] The following examples are presented in order to more fully illustrate the preferred embodiments of the invention. They should in no way be construed, however, as limiting the broad scope of the invention.

EXPERIMNTAL DETAILS SECTION EXAMPLE 1- :0 ANROGENIC AND ANABOLIC ACTVITY OF COMPOTNDS 14 [000191] Binding affinities of select B-ring F, C1, Br, lated SARMS were detemnined and are represented in Table 1: TABLE. TABLE 1: 3 0 2 N Br '462 5.3 12.6± 1.8- 0L 0 4 0 2 N i 510 2.7 23 4 1.6 H \OH 0 V0 EXPERIMENTAL METHODS [000192] Animals. Immature male Sprague-Dawley rats, weighing 90 to 100g, were purchased from Harlan Biosciences (Indianapolis, IN). The animals were maintained on a 12-hour light-dark cycle with food and water available ad libitum. The animal protocol was reviewed and approved by the Institutional Laboratory Animal Care and Use Committee.

[000193] Study Design. Rats were randomly distributed into treatment groups groups. One day prior to the start of drug treatment, animals were individually removed from the cage, weighed and anesthetized with, an intraperitoneal dose of ketamine/xylazine (87/13 mg/kg; approximately 1 mL per kg). When appropriately anesthetized no response to toe pinch), the animals'- ears were marked for identification purposes. Animals were then placed on a sterile pad and their abdomen and scrotum washed with betadine and 70% alcohol. The testes were removed via a midline scrotal incision, with sterile suture being used to ligate supra-testicular tissue prior to surgical removal of each testis. The surgical wound site was closed with sterile stainless steel wound clips, and the site cleaned with betadine. The animals were allowed to recover on a sterile pad (until able to stand) and then returned to their cage.

[000194] Twenty-four hours later, animals were re-anesthetized with ketamine/xylazine, and an Alzet osmotic pump(s) (model 2002) was placed subcutaneouly in the scapular region. In this instance, the scapular region was shaved and cleaned (betadine and alcohol) and a small. incision (1 cm) made using a sterile I0 scalpel. The osmotic pump was inserted and the wound closed with a sterile stainless o steel wound clip. Animals were allowed to recover and were returned to their cage.

ciq Q Osmotic pumps contained the appropriate treatment dissolved in polyethylene glycol 300 (PEG300). Osmotic pumps were filled with the appropriate solution one day prior to S implantation. Animals were monitored daily for signs of acute toxicity to drug treatment lethargy, rough coat).

O- [000195] After 14 days of drug treatment, rats were anesthetized with o 10 ketamine/xylazine. Animals were then sacrificed by exsanguinations under anesthesia.

ID A blood sample was collected by.venipuncture of the abdominal aorta, and submitted for o complete blood cell analysis. A portion of the blood was placed in a separate tube, centrifuged at 12,000g for 1 minute, and the plasma layer removed and frozen at The ventral prostates, seminal vesicles, levator ani muscle, liver, kidneys, spleen, lungs, and heart were removed, cleared of extraneous tissue, weighed, and placed in vials containing 10% neutral buffered formalin. Preserved tissues were sent to GTx, Inc. for histopathological analysis.

[000196] For data analysis, the weights of all organs were normalized to body weight, and analyzed for any statistical significant difference by single-factor ANOVA.

The weights of prostate and seminal vesicle were used as indexes for evaluation of androgenic activity, and the levator ani muscle weight was used to evaluate the anabolic activity.

RESULTS

[000197] The androgenic and anabolic activities of compounds 1-4 were examined in a castrated rat model after 14 days of administration. The results are shown in Figure 1 A-D as a percent of the Intact Contol (not castrated, untreated). Omg/day denotes Castrated Controls (castrated, untreated).

[000198] As shown in Figure 1 the weights of prostate, seminal vesicle, and Slevator ani muscle in castrated rats .decreased -significantly, due to the ablation of o endogenous androgen production. Treatment with increasing dosages of compounds 1-4 (Figure 1 A-D respectively) resulted in a tissue-selective increase in levator ani muscle weights, with little or no stimulation of prostate and seminal vesicle growth the c prostate and seminal vesicle weights were less than 40% of that observed in intact animals for compound,2, and less than 20%/ for compounds 1, 3 and Thus these compounds showed little potency and intrinsic activity in increasing the weights of prostate and seminal vesicle, but a great potency and intrinsic activity in increasing the weight of levator ani muscle. Particularly, compound 2 was able to maintain the levator N ani muscle weight of castrated animals in the same level as that of intact animals. Thus, O compounds 1-4, are potent nonsteroidal anabolic agents. This is a significant improvement over previous compounds, in that these compound selectively stimulate muscle growth and other anabolic effects while having .ess effect on the prostate and seminal vesicles. This may be particularly relevant in aging men with concerns related to the development or progression of prostate cancer.

EXAMPLE 2 SANDROGENIC AND ANABOLIC ACIVITY OF COMPOUND [000199] The binding afinitiy of select compound 5 is represented in Table 2: TABLE 2: [000200] The androgenic and anabolic activities of compound 5 was examined in a castrated rat model after 14 days of administration, using the method outlined in Examplel1 above.

[000201] As shown in Table 3 and in Figure'2, compound 5 demonstrated tissueselective pharmacological effects in castrated male rats, with higher efficacy in anabolic tissues levator ani) as compared to androgenic tissues prostate and seminal vesicles). Compound 5 demonstrated little pharmacologic activity in the prostate (8.7± 1.39% of intact at 1.0 mg/day dose) and sminal vesicles (10.71 0.91% of intact at mg/day dose), suggesting that it acts as a weak partial agonist in these tissues.

Importantly, compound ,5 demonstrates highly efficacious anabolic activity at mg/day.dose, returning the levator ani muscle to 75.21 9.51% of that observed in intact animals. TABLE 3. Average (Meant Organ Weights Prostate Levator Ani Seminal Vesicles Intact Control 100 11.28 100 12.12 100 2.48 Castrated Control 7.6 0.68 45.9 10.84 8.4 1.05 0.10 mg/day 6.4 0.82 54.9 5.77 8.8 1.18 0.25 mg/day 5.7 0.61 61.0 5.23 7.6 1.37 S0.50 mg/day 6.2 ±0.56 :55.0 9.23 -93 1.57 0.75 mg/day 7.6 0.74 68.9 8.46 9.8 3.65 1.00 mg/day 8.7 4 1.39 75.2 9.51 10.7 0.91 EXAMPLE 3 ANDROGENIC AND ANABOLIC ACTIVITY OF COMPOUND 6 [000202] The binding afinitiy of select compound 6 is represented in Table 4: TABLE 4: Name Structure MW Ki enH [0002033 The androgenic and anabolic activities of compound 6 was examined in a S castrated rat model after 14 days of administration, using the method outlined in o Example 1 above.

o [000204] As shown in Figure 3, the weights of prostate, seminal vesicle, and C levator ani muscle in castrated, vehicle-treated rats decreased significantly, due to the ablation of endogenous androgen production. Exogenous administration of testosterone propionate,, an androgenic and anabolic steroid, increased the weights of prostate,.

seminal vesicle, and levator ani muscle in castrated rats in a dose-dependent manner.

Treatment with compound 6 resulted in dose-dependent increases in prostate, seminal vesicle and levator ani muscle weights. Compared with testosterone propionate, compound 6 showed lower potency and intrinsic activity ini increasing the weights of Sprostate and seminal vesicle, but a greater potency and intrinsic activity in increasing the weight of levator ani muscle. Particularly, compound V, at a dose as low as 0.3 mg/day, was able to maintain the levator ani muscle weight of castrated animals in the same level as that of intact animals. Thus, compound 6 is a potent nonsteroidal anabolic agent with less androgenic activity but more anabolic activity than testosterone propionate. As in compounds 1-5 above, this is a significant improvement in that this compound selectively stimulates muscle growth and other anabolic effects while having less effect on the prostate and seminal vesicles.

EXAMPLE 4 IDBinding Affinties of Selective Androgen Receptor Modulators 0 IC [000205J The in-vitro androgen receptor binding affinity of other SARM 4 5 compounds was studied and the results are presented in Table TABLE Name Siructure MW Ki

O

o 7 NC F 348.1 4.5 10.11

ID

0

NHC

ci1 3 C bE-'F Average DHT Ki value: 0.3 6±0.15 N0 EXAMPLE

O

o Nonsteroidal Ligands with Androgenic and Anabolic Activity 4 5 [000208] The in-vivo efficacy and acute toxicity of four nonsteroidal androgens c- (compounds 1, 21, 22 and 23) in rats was examined. In-vitro assays established that these compounds bind the androgen receptor with very high affinity. The structures and names of the four compounds are presented below: SC

''OH

Compound 1 R= F Compound 21 R=NHCOCH 3 Compound 22 R=COCH 3 o Compound 23 R=COC 2 EXPERIMENTAL METHODS [0002071 Materials. The S-isomers of 1, 21, 22 and 23 and R-isomer of compound 1 were synthesized in accordance with the scheme as set forth in Figure 7. Testosterone propionate polyethylene glycol 300 (PEG300, reagent grade) and neutral buffered formalin (10% w/v) were purchased from Sigma Chemical Company (St Louis, MO).

Alzet osmotic pumps (model 2002) were purchased from Alza Corp. (Palo Alto, CA).

[000208] Animals. Immature. male Sprague-Dawley rats, weighing 90 to 100g, were purchased from Harlan Biosciences (Indianapolis, IN). The animals were maintained on a 12-hour light-dark cycle with food and water available ad libitum. The animal protocol was reviewed and approved by the Institutional Laboratory Animal Care and Use Committee.

IND [000209] Study Design. Rats were randomly distributed into twenty-nine .(29) 0 groups, with 5 animals per group. Treatment groups are described in Table 6. One day- Sprior to the start of drug treatment, animals in groups 2 through 29 were individually removed from the cage, weighed and -anesthetized with: an intraperitoneal ;dose of en ketamine/xylazine (87/13 mg/kg; -approximately 1 mL per kg). When appropriately anesthetized no response to toe pinch), the animals' ears were marked for identification purposes. Animals were then placed on a sterile pad and their abdomen and scrotum washed with betadine and 70% alcohol. The testes were removed via a 0 o 10 midline scrotal incision, with sterile suture being used to ligate supra-testicular tissue I0 prior to surgical removal of each testis. The surgical wound site was closed with sterile 0 o stainless steel wound clips, and the site cleaned with betadine. The animals were allowed to recover on a sterile pad (until able to stand) and then returned to.their cage.

[000210] Twenty-four hours later, animals .in groups 2 through 29 were reanesthetized with ketamine/xylazine, and an Alzet osmotic pump(s) (model 2002) was placed subcutaneouly in the scapular region. '-In this instance, the scapular region was shaved and cleaned (betadine and alcohol) and a small incision (1 cm) made using a sterile scalpel. The osmotic pump was inserted and the wound closed with a sterile stainless steel wound clip. Animals were allowed to recover and were returned to their cage. Osmotic pumps contained the appropriate treatment (designated in Table 1) dissolved in polyethylene glycol 300 (PEG300). Osmotic pumps were filled with the appropriate solution one day prior to implantation. Animals were monitored daily for signs of acute toxicity to drug treatment lethargy, rough:coat).

[000211] After 14 days of drug treatment, rats were anesthetized with ketamine/xylazine. Animals were then sacrificed by exsanguinations under anesthesia.

A blood sample was collected by venipuncture of the abdominal aorta, and submitted for complete blood cell analysis. .A portion of the blood was placed in a separate tube, centrifuged at 12,000g for 1 minute, and the plasma layer removed and frozen at The ventral prostates, seminal vesicles, levator ani muscle, liver, kidneys, spleen, lungs, and heart were removed, cleared of extraneous tissue, weighed, and placed in vials containing 10% neutral buffered formalin. Preserved tissues were sent to GTx, Inc. for NO histopathological analysis.

[000212] For data analysis, the weights of all organs were nomnalized to body

C)

4 5 weight, and analyzed for any statistical significant difference by single-factor ANOVA.

j The weights of prostate and seminal vesicle were used as indexes for evaluation of androgenic activity, and the levator ani muscle weight was used to evaluate the anabolic activity. 0 10 RESULTS [000213] The androgenic and anabolic activities the S isomers of compounds 1, 21, 22 and 23, and the R isomer of compound 1 were examined in a castrated rat model after 14 days of administration. Testosterone propionate, at increasing doses, was used as the positive control of anabolic and androgenic effects.' [000214] :As shown in Figure 4, the weights of prostate, seminal vesicle, and levator ani muscle in castrated, vehicle-treated rats decreased significantly, due to the ablation of endogenous androgen production. Exogenous administration of testosterone propionate, an androgenic and anabolic steroid, increased the weights of prostate, seminal vesicle, and levator ani muscle in castrated rats in a dose-dependent manner.

The R-isomer of compound 1, and S-isomers of compounds 22 'and 23 showed no effect on the weights of prostate, seminal vesicle, and levator ani muscle in castrated animals (data not shown). The S-isomer of compound 1 (Figure 4) resulted in dose-dependent increases in prostate, seminal vesicle and levator ani muscle weights.

[000215] Compound 1 but showed great tissue selectivity. Compound 1 significantly increased levator ani muscle weights, but showed little to no ability to stimulate prostate and seminal vesicle growth the prostate and seminal vesicle weights were less than 20% of that observed in intact animals or in animals treated with testosterone propionate).

[000216] In suinmmary, Compound 1 showed selective anabolic activity in IND comparison with testosterone propionate, an androgenic and anabolic steroid. The tissue- 0 S selective activity is actually one of the advantages of nonsteroidal androgens in terms of d) anabolic-related applications. c r [000217] None of the examined compounds produced significant effect on body weight or the weights of other organs liver, kidneys, spleen, lungs and heart). Nor 0> did any compound produce any signs of acute toxicity, as gauged by diagnostic hematology tests and visual examination of animals receiving treatments. Importantly, o 10 compound 21 did not suppress the production of luteinizing hormone (LH) or follicle Sstmulating hormone (FSHI) at a dose of 0.3 mg/day a dose that exhibitedmaximal o anabolic effects). [000218] These studies showthe.discovery ofcompound 1 is a member of a class of selective androgen receptor modulators (SARMS) that demonstrates potent anabolic effects muscle growth) with less androgenic activity prostatic growth). This new class of drugs has several advantages over non-selective androgens, including potential therapeutic applications in males and females for modulation of fertility, erythropoiesis, osteoporosis, sexual libido and in men with or at high risk for prostate cancer... [000219] Further, Figures 5 and 6 demonstrate the effects of compound 1 and compound 21 on LH and FSH levels in rats.. These results further demonstrate the novelty of thr'se- SAiRMs, due to- their differential effects on these reproductive hormones, thus demonstrating the tissue-specific pharmacologic activity. In Figure LH levels in castrated animals treated with .TP and compound 1 were significantly lower than those of untreated animals castrated controls) at doses greater than or equal to.

0.3 mg/day. However, higher doses 0.5 mg/day or higher) of compound 21 were required before significant decreases in LH levels were observed. Thus, compound 21 does not suppress LH levels at doses that are capable of eliciting maximal stimulation of levator ani muscle growth. In Figure 6, FSH levels in castrated animals treated with compound 1 were significantly lower than those of untreated animals castrated controls) at doses of 0.5 mg/day or higher. Similarly, lower FSH levels were observed in animals treated with TP. However, only this difference was only significant at a dose of 0.75 mg/day. FSH levels in animals treated with compound 21 were not significantly different from those of untreated animals at any dose level-tested. Thus, compound 21 does not suppress FSH levels at doses that are capable of eliciting maximal stimulation of levator ani muscle growth. oo[000220] Table 6. Animals Groups and Experimental Design.

i i Group Castrated? Drug Dose of animals 1 No None None 2 Yes None Vehicle only 3 Yes Testosterone 0.1 mg/day 4 Yes Testosterone 0.3.mg/day Yes 'Testosterone 0.5 ig/day 6 Yes Testosterone 0.75 ing/day 7 Yes Testosterone 1.0 mg/day 8 Yes R-I 1.0 mg/day 9 Yes S-1 '1 mg/day .Yes S- 1 0.3 mg/day, 11 Yes S- 1 0.5 mg/day. .12 Yes S- 1 0.75 mg/day 13 Yes S-1 1.0 mg/day 14 Yes S- 1 0.1 mg/day Yes S-21 0.3 mg/day 16 Yes S-21 0.5 mg/day 17 Yes S-21 0.75 mg/day 18 Yes S-21 1.0 mg/day 19 Yes S- 22 0.1 mg/day Yes S- 23 0.3 mg/day 21 Yes S-23 0.5 mg/day 22 Yes S- 23 0.75 mg/day 23 Yes S-23 1.0 mg/day 24 Yes S-21 0.1 mg/day Yes S- 21 0.3 mg/day 26 Yes S- 21 0.5 mg/day 27 Yes .S-21 0.75 mg/day 28 Yes S-21 1.0 mg/day 29 Yes None Vehicle only EXAMPLE 6 SYNTHETIC PROCEDURES

NO

O [000221] (2R)-l-Methacryloylpyrrolidin-2-carboxylie Acid (R-129). D-Proline (R-128, 14.93 g, 0.13 mol) was dissolved in 71 mL of 2 N NaOH and cooled in an ice bath; the resulting alkaline -solution was diluted with acetone. (71. mL). An acetone mn solution (71 mL) of metacryloly chloiide 127 (13.56 g, 0.13 mol) and 2N NaOH solution (71 mL) were simultaneously added over 40 min to the aqueous, solution of D-proline in an ice bath. The pH of the mixture was kept at 10-11°C during the addition of the metacryloly chloride. After stirring (3 h, room temperature), the mixture was evaporated in vacuo at a temperature at 35-45 °C to remove acetone. The resulting solution was IN washed with ethyl ether and was acidified to pH 2 with concentrated HC1. The acidic 0 mixture was saturated with NaCl and was extracted with EtOAc (100 mL x The combined extracts were dried over Na 2 SO4, filtered through Celite, and evaporated in vacuo to give the crude product as a polorless oil. Recrytallzation of the oil from ethyl ether and hexanes afforded 16.2 of the desired compound as colorless crystals: mp 102-103 "C (lit. [214] mp 102.5-103.5 the NMR spectrum of this compound demonstrated the existence of two rotamers f the title compound. 'H NMR (3010 MHz, DMSO-d6) 5 5.28 and 5.15 for the first rotamer, 5.15 and 5.03 for the second rotamer (totally 2H for both rotamers, vinyl CH), 4.48-4.44 for the first rotamer, 4.24-4.20 for.the second rotamer (totally 1H for both rotamers, CH at the chiral canter), 3.57-3.38 2H, CH2), 2.27-2.12 (1H, CH), 1.97-1.72 6H, CH2, CH, Me); 3C NMR (75 1Hz, DMSO-d6) B for major rotamer 173.3, .169.1, 140.9, 116.4, 58.3, 48.7, 28.9, 24.7, 19.5: for minor rotamer 174.0, 170.0, 141.6, 115.2, 60.3, 45.9, 31.0, 22.3, 19.7; IR (KBr) 3437 1737 1647 (CO, COO-), 1584, 1508, 1459, 1369, 1348, 1178 cmTa [aCC 2 6 +80.80 (c 1, MeOH); Anal. Calcd. for CgHuNO 3

C

59.00, H 7.15, N 7.65. Found: C 59.13, H 7.19, N 7.61.

[000222] (3R,8aR)-3-Bromomethyl-3-methyl-tetrahydro-pyrrolo[2,c] [1,4] oxazine-1,4-dione R-130). A solution of NBS (23.5g, 0.132 mol) in 100 mL of DMF was added dropwise to a stirred solution of compound R-129 (16.1g, 88 mmol) in mL of DMF under argon at room temperature, and the resulting mixture was stirred 3 days. The solvent was removed in vacuo, and a yellow solid was precipitated. The solid I the residue was diluted with saturated NaHCO 3 solution, and extracted with ethyl ether IND(100 mL x Combined extracts were dried over anhydrous NazSO4 1fitered through 0 o Celite, and purified by flash chromatography on silica gel, using methylene chloride as N eluent to afford 18.0 g of the desired compound: mp 98-100 C 0.2 silica gel, CH 2

C

2 'H NMR (300 Mfz, DMSO-d) 8 10.54 1H, NH), 8.54 J= 2.1 Hz, 1HL, ArH), 8.34 (dd, J= 9.0 Hz, J= 2.1 HIz, 11H, ArH), 8.18 J =9.0 z, 1I, ArH), 6.37 1H, OH), 3.82 J 10.4 Hz, 1H, CH1Ea), 3.58 J 10.4Hz, 11K CHHb), 1.48 31H, Me); '3C NMR (75 MHz, DMSO-ds) 8 173.6 143.0, 127.2, 123.2, 122.6 J 33.0 Hz), 122.0 J 271.5 Hz), 118.3 (q,J Iz), 74.4, 41.4,24.9; jR (KBr) 3344 1680 1599, 1548 Ar), 1427, 1363, 1161'cm'; MS o (ESI): m/z 370.8 Anal. Caled. for CujHjiBrN 2 0 4 C 35.60, H 2.72, N 7.55. Found: S C 35.68, H 2-72, N 7.49.

[000226] N-[4-nitro-3-trifluoromethyl)phnyl]-(2S)-3-[4-(aeetyamino) phen oxy]-Z-hydroxy-2-methylpropanamide (S-147, Compound 21). The title compound was prepared from compound R-132 (0.37 g, 1.0 mmol), 4-acetamidophenol (0.23 g, mmol) K2C0O 3 (0.28 g, 2.0 mmol), and 10% of benzyltributylammonium chloride as a phase transfer catalyst in 20 mL of methyl ethyl ketone was heated at reflux ovemrnight under argon. The reaction was followed by TLC, the resulting mixture was filtered zo through Celite, and concentrated in vacuo to dryness. Purification by flash column chromatography on silica gel (hexanes-ethyl acetate, 3:1) yielded 0.38 g (Rf 0.18 hexanes-ethyl acetate, 3:1) of the desired compomund as a light yellow powder: mp 70-74 0C; The solid can be recrystalized from ethyl acetate and hexane); 'H NMR (300 MHz, DMSO-d) 8 10.62 1H, NM), 9.75 1H, NH), 8.56 J= 1.9 Hz, IN, ArH), 8.36 (dd, J 9.1 Hz, J= 1.9 Hz, 1H, Ar), 8.18 JI 9.1 Hz, 111, ArH), 7.45-7.42 (m, 2H, ArE), 6.85-6.82 21H, ArT), 6.25 11, OH), 4.17 J 9.5 Hz, 1H, CH,), 3.94 J 9.5 Hz, 1H, CHHb), 1.98 3H, Me), 1.43 31, Me); "sC NMR (75 MHz, DMSO-d) 8174.6 167.7, 154.2, 143.3, 141.6, 132.8, 127.4, 123.0, 122.7 J= 33.0 Hz), 122.1 J 271.5 Hz), 120.1, 118.3 J 6.0 Hz), 114.6, 74.9, 73.8, 23.8, 23.0; IR (KBr) 3364 1668 1599,1512 Ar), 1457,1415,1351, 1323, 1239, 1150 1046 cm'; MS (ESI): m/z 464.1 (M+Na)f; Anal. Caled. for C 19

HISF

3

N

3 0 6 C 51.71, H 4.11, N 9.52. Found: C 52.33, H 4.40, N9.01.

ON

F"-

0 i o 0 0 ci [000226] The synthesis of the various ether analogs of Compound 21, such as, but not limited to, compounds 1-4, 22 and 23 provided. herein, utilizes the common intermediate that is the final.reaction step. Bromo-intennediates are used which allow various phenolic compounds to displace the bromide to give the .desired ether product.

Bromohydrin was converted to an epoxide and to open the.epoxide to give the same desired ether product.

[000227] It will be appreciated by a person skilled in the art that the present invention is not limited by what has been particularly shown and described hereinabove, Rather, the scope of the invention is defined by the claims that follow: 75 NO [000228] In the claims which follow and in the 0 Spreceding description of the invention, except where the Scontext requires otherwise due to express language or Snecessary implication, the word "comprise" or variations C 5, such as "comprises" or "comprising" is used in an inclusive sense, ie. to specify the presence of the stated features but not to preclude the presence or addition of further features in various embodiments of the invention.

o [000229] It is to be clearly understood that I 10 although prior art publication(s) are referred to herein, 0 this reference does not constitute an admission that any of these documents forms part of the common general knowledge in the art in Australia or in any other country.

H: \karR\Keep\Specl\ECCl12.doc 23/02/06

Claims (11)

1. 2- A selective androgen receptor modulator (SARM) compound represented by the structure of formula (11A): wherein (11A) X is 0; Z is NO 2 ON, COR, or CONHR; Y is 1, OF 3 Br, CI. or SnR 3 R isan elky group or OH; and Q is ON; E:\MiaraR,\Kcep\Speci\P600G2-doc 23/02/06 77 or its analog, isomer, metabolite, derivative, pharmaceutically acceptable salt, pharmaceutical product, polymorph, crystal, impurity, N-oxide, hydrate or any combination thereof.
2. 3. The selective androgen modulator compound according to claim 1, wherein Z is NOa.
3. 4. The selective androgen modulator compound according to claim 1, wherein Z is CN. The selective androgen modulator compound according to claim 1, wherein Y is CF. o 6. The selective androgen modulator compound according to claim 1, Swherein Q is CN.
4. 7. The selective androgen modulator compound according to claim 1, wherein Z is NOg, Y is CF,, and Q is CN.
5. 8. The selective androgen modulator compound according to claim 1, wherein Z is CN, Y is CF 3 and Q is CN.
6. 9. A selective androgen receptor modulator (SARM) compound represented by the structure of formula (111): H 3 C OH (M H:\IaraF\Keep\Speci\P6(j42.doc 23/02/06 78 O O
7. 10. A composition comprising the selective androgen receptor Smodulator compound of claims 1 or 9, and/or its analog, isomer, Smelabolite, derivative, pharmaceutically acceptable salt, pharmaceutical product, polymorph, crystal, impurity. N-oxide, hydrate or any combination thereof; and a suitable carrier or diluent.
8. 11. A pharmaceutical composition comprising an effective amount of o the selective androgen receptor modulator compound of claims 1 Cor 9, and/or its analog, isomer, metabolite, derivative, Spharmaceutically acceptable salt, pharmaceutical product, Spolymorph, crystal, impurity, N-oxide, hydrate; and a pharmaceutically acceptable carrier, diluent or salt
9. 12. A method of binding a selective androgen receptor modulator compound to an androgen receptor, comprising the step of contacting the androgen receptor with the selective androgen receptor modulator compound of claim 1 and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, polymorph. crystal, impurity, hydrate, N- oxide or any combination thereof, in an amount effective to bind the selective androgen receptor modulator compound to the androgen receptor.
10. 13. A method of contraception in a male subject, comprIg-l administering to said subject the selective androgen receptor modulator compound of claim 1 and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, pharmaceutical product, polymorph. crystal, impurity, hydrate, N- oxide or any combination thereof, in an amount effective to suppress sperm production in said subject, thereby effecting contraception in said subject.
11. 14. A method of hormone therapy comprising the step of contacting an androgen receptor of a subject with the selective androgen receptor modulator compound of claim 1 and/or its analog, derivative, isomer, metabolite, pharmaceutically acceptable salt, H:\MaraR\Keep\Specl\60042.doc 23/02/0E 79