AT93541T - Hybrid polypeptides and methods of manufacture for their. - Google Patents

Hybrid polypeptides and methods of manufacture for their.

Info

Publication number
AT93541T
AT93541T AT86305057T AT86305057T AT93541T AT 93541 T AT93541 T AT 93541T AT 86305057 T AT86305057 T AT 86305057T AT 86305057 T AT86305057 T AT 86305057T AT 93541 T AT93541 T AT 93541T
Authority
AT
Austria
Prior art keywords
manufacture
methods
hybrid polypeptides
polypeptides
hybrid
Prior art date
Application number
AT86305057T
Other languages
German (de)
Inventor
Gregory L Gray
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
Priority to US75226785A priority Critical
Application filed by Genencor Int filed Critical Genencor Int
Priority to EP19860305057 priority patent/EP0208491B1/en
Publication of AT93541T publication Critical patent/AT93541T/en
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25025597&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AT93541(T) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
AT86305057T 1985-07-03 1986-06-30 Hybrid polypeptides and methods of manufacture for their. AT93541T (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US75226785A true 1985-07-03 1985-07-03
EP19860305057 EP0208491B1 (en) 1985-07-03 1986-06-30 Hybrid polypeptides and process for their preparation

Publications (1)

Publication Number Publication Date
AT93541T true AT93541T (en) 1993-09-15

Family

ID=25025597

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86305057T AT93541T (en) 1985-07-03 1986-06-30 Hybrid polypeptides and methods of manufacture for their.

Country Status (8)

Country Link
US (1) US5093257A (en)
EP (1) EP0208491B1 (en)
JP (1) JPH0829091B2 (en)
AT (1) AT93541T (en)
AU (1) AU5945086A (en)
CA (1) CA1312836C (en)
DE (2) DE3688920T4 (en)
IE (1) IE59875B1 (en)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234823A (en) * 1986-06-30 1993-08-10 Novo Industri A/S Chimeric enzymes
DK311186D0 (en) * 1986-06-30 1986-06-30 Novo Industri As enzymes
US4966846A (en) * 1987-10-01 1990-10-30 W. R. Grace & Co.-Conn. Molecular cloning and expression of a vibrio proteolyticus neutral protease gene
ES2092488T3 (en) * 1989-06-29 1996-12-01 Gist Brocades Nv Mutant enzyme reduced stability under industrial application.
CA2030554C (en) * 1989-06-29 2001-08-28 Wilhelmus J. Quax Mutant microbial .alpha.-amylases with increased thermal, acid, and/or alkaline stability
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
NL8902010A (en) * 1989-08-04 1991-03-01 Nl Zuivelonderzoek Inst DNA fragment with at least one protease gene, such a DNA fragment containing cloneringsvektor, a host cell transformed with such a built-up cloneringsvektor, as well as the proteases produced by such host cells, respectively. -prepared products such as foodstuffs.
AT318304T (en) 1993-10-08 2006-03-15 Novozymes As amylase
AU7807494A (en) * 1993-10-08 1995-05-04 Novo Nordisk A/S Amylase variants
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5830837A (en) * 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
US6093562A (en) * 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
AR000862A1 (en) 1995-02-03 1997-08-06 Novozymes As Variants of an O-amylase, a method for producing the same, a DNA and an expression vector, a cell transformed by dichaestructura DNA and vector, a detergent additive, detergent composition, a composition for laundry and a composition for removing the
US5958739A (en) * 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
AT216427T (en) * 1995-08-11 2002-05-15 Novozymes As Method for the production of polypeptide combs
US5741767A (en) * 1995-11-16 1998-04-21 Lever Brothers Company, Division Of Conopco, Inc. Peracid based dishwashing detergent composition
US6077697A (en) 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US20030033617A1 (en) 1996-04-10 2003-02-13 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
US6159687A (en) * 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6080568A (en) * 1997-08-19 2000-06-27 Genencor International, Inc. Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis
CN101125873A (en) * 1997-10-24 2008-02-20 茵维特罗根公司 Recombinational cloning using nucleic acids having recombination sites
CA2307016A1 (en) * 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
DK2270234T3 (en) 1997-12-08 2013-06-03 California Inst Of Techn Method for Preparation of Polynucleotide and Polypeptide Sequences
CA2344619C (en) 1998-10-26 2012-01-03 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US7670823B1 (en) 1999-03-02 2010-03-02 Life Technologies Corp. Compositions for use in recombinational cloning of nucleic acids
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DK1250453T3 (en) 1999-12-10 2008-08-11 Invitrogen Corp Use of multiple recombination sites with unique specificity in recombination cloning
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
HU0203751A3 (en) 2000-01-10 2005-01-28 Maxygen Holdings Ltd Redwood C G-csf conjugates
CA2739933A1 (en) 2000-02-11 2001-08-16 Bayer Healthcare Llc Factor vii or viia-like molecules
US6248541B1 (en) 2000-04-21 2001-06-19 Genencor International, Inc. Screening under nutrient limited conditions
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
JP2004504837A (en) 2000-07-28 2004-02-19 ヘンケル・コマンディットゲゼルシャフト・アウフ・アクチエンHenkel Kommanditgesellschaft Auf Aktien A novel amylolytic enzyme extracted from Bacillus sp. A7-7 (DSM12368) and a washing and cleaning agent containing the novel amylolytic enzyme
US7087415B2 (en) * 2000-07-31 2006-08-08 Athena Biotechnologies, Inc. Methods and compositions for directed gene assembly
US20020155574A1 (en) * 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
US20020155439A1 (en) * 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
US7407677B2 (en) 2001-02-21 2008-08-05 Verenium Corporation Enzymes having alpha amylase activity and methods of use thereof
US7659102B2 (en) 2001-02-21 2010-02-09 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
CA2444195A1 (en) * 2001-04-19 2002-10-31 Invitrogen Corporation Compositions and methods for recombinational cloning of nucleic acid molecules
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
IL159268D0 (en) 2001-06-20 2004-06-01 Nuevolution As Templated molecules and methods for using such molecules
DE10138753B4 (en) * 2001-08-07 2017-07-20 Henkel Ag & Co. Kgaa Detergents and cleaners with hybrid alpha-amylases
DE60331644D1 (en) * 2002-04-22 2010-04-22 Novozymes Inc Method for producing variants of a dna sequence in filamentous mushrooms
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
AU2003253992A1 (en) * 2002-07-18 2004-02-09 Robert P. Bennett Viral vectors containing recombination sites
US7560260B2 (en) * 2002-07-25 2009-07-14 Bio-Rad Laboratories, Inc. Compositions with polymerase activity
WO2004042006A2 (en) 2002-10-31 2004-05-21 Diversa Corporation Amylases, nucleic acids encoding them and methods for making and using them
US20040214194A1 (en) * 2003-04-17 2004-10-28 Mj Bioworks, Inc. Methods of making hybrid proteins
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
EP1697534B1 (en) 2003-12-01 2010-06-02 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2005111203A2 (en) * 2004-04-08 2005-11-24 Genencor International, Inc. MUTANT α ΑMYLASES
US7332319B2 (en) * 2004-05-27 2008-02-19 Genencor International, Inc. Heterologous alpha amylase expression in Aspergillus
WO2006073839A2 (en) * 2004-12-30 2006-07-13 Genencor International, Inc. Acid fungal proteases
US7968318B2 (en) * 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
US20080299622A1 (en) * 2007-02-07 2008-12-04 Paulson Bradley A Starch Hydrolysis Using Phytase with an Alpha Amylase
WO2008112282A1 (en) 2007-03-14 2008-09-18 Danisco Us, Inc., Genencor Division Production of ethanol from barley and ddgs containing reduced beta-glucan and phytic acid
JP2011510682A (en) * 2008-02-06 2011-04-07 ダニスコ・ユーエス・インク A pH-free system for the production of fermentable sugars and alcohols
JP5702714B2 (en) * 2008-04-30 2015-04-15 ダニスコ・ユーエス・インク Novel chimeric alpha amylase mutant
JP2012515532A (en) 2009-01-20 2012-07-12 ラモット アット テル アビブ ユニバーシティ, リミテッド MIR-21 promoter driven target cancer treatment
CA2756596A1 (en) 2009-03-27 2010-09-30 Proyecto De Biomedicina Cima, S.L. Methods and compositions for the treatment of cirrhosis and liver fibrosis
WO2014092960A1 (en) 2012-12-11 2014-06-19 Danisco Us Inc. Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4336336A (en) * 1979-01-12 1982-06-22 President And Fellows Of Harvard College Fused gene and method of making and using same
NZ195980A (en) * 1980-01-08 1984-10-19 Biogen Nv Dna sequences,recombinant dna molecules and processes for producing leukocyte interferon-like polypeptides;pharmaceutical and veterinary compositions
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
US4493893A (en) * 1981-01-15 1985-01-15 Cpc International Inc. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis

Also Published As

Publication number Publication date
IE59875B1 (en) 1994-04-20
DE3688920T4 (en) 1995-08-31
US5093257A (en) 1992-03-03
JPS6283891A (en) 1987-04-17
AU5945086A (en) 1987-01-08
EP0208491A2 (en) 1987-01-14
JPH0829091B2 (en) 1996-03-27
DE3688920T2 (en) 1993-12-23
EP0208491B1 (en) 1993-08-25
IE861785L (en) 1987-01-03
EP0208491A3 (en) 1988-09-21
CA1312836C (en) 1993-01-19
DE3688920D1 (en) 1993-09-30

Similar Documents

Publication Publication Date Title
DE3582639D1 (en) In vitro cell culture-system and process.
DE69131539T2 (en) Polypeptides having fibrinbindungsdomäne, uses and methods for their preparation
DE3516587A1 (en) Porous siliciumcarbidsinter and process for its manufacture
DE3785690T2 (en) Electrophoretic display particles and methods for their preparation.
AT47323T (en) Muskeluebungs- and diagnosegeraet and procedures.
DE3280479D1 (en) Recombinant DNA Means and methods
DE3852738T2 (en) Thermoplastic composition and process for their preparation.
NO171280C (en) Smoerefettblanding and method for preparation thereof
DE3780925T2 (en) Nasal formulations and methods for their preparation.
DE3577251D1 (en) Sealed lead-acid battery and process for its production.
DE3769161D1 (en) Aethercarboxylatreinigungsverstaerker and process for their preparation.
DE3617896A1 (en) Bone graft material and process for its manufacture
DE3616578A1 (en) Alumina verbundkoerper and process for its preparation
AT55099T (en) Leichtisolierplatten and process for their preparation.
DE3854644D1 (en) Methods and devices for cell determination.
DE3776300D1 (en) Gas-tight accumulator and method for manufacturing its electrode.
DE3514058A1 (en) Composite foam-seat cushion and process for its manufacture
DE3586573D1 (en) Signaluebertragungssystem and procedures.
NO174264C (en) Våtpressepapirmaskinfilt and method for preparation thereof
DE3683292D1 (en) Interchangeable waermetintenstrahlkomponente and waermeschallstrahlverbundverfahren for their preparation.
DE3541766A1 (en) Rueckstrahlermaterial and process for its manufacture
AT79803T (en) Composite powder, verbundkoerper and process for their preparation.
DE3775486D1 (en) Thermoplastic rubber-polymer alloy and process for their preparation.
DE3586402T2 (en) Protein composition with coagulation-effective and process for their manufacture.
AT77619T (en) 3-aryluracils and their use for unkrautbekaempfung.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
RZN Patent revoked