AT534731T - Compositions and methods for random mutagenesis of nucleic acids - Google Patents

Compositions and methods for random mutagenesis of nucleic acids

Info

Publication number
AT534731T
AT534731T AT02746439T AT02746439T AT534731T AT 534731 T AT534731 T AT 534731T AT 02746439 T AT02746439 T AT 02746439T AT 02746439 T AT02746439 T AT 02746439T AT 534731 T AT534731 T AT 534731T
Authority
AT
Austria
Prior art keywords
compositions
methods
nucleic acids
random mutagenesis
mutagenesis
Prior art date
Application number
AT02746439T
Other languages
German (de)
Inventor
Holly Hogrefe
Janice Cline
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US29434101P priority Critical
Application filed by Stratagene California filed Critical Stratagene California
Priority to PCT/US2002/016280 priority patent/WO2002097078A1/en
Publication of AT534731T publication Critical patent/AT534731T/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
AT02746439T 2001-05-30 2002-05-23 Compositions and methods for random mutagenesis of nucleic acids AT534731T (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US29434101P true 2001-05-30 2001-05-30
PCT/US2002/016280 WO2002097078A1 (en) 2001-05-30 2002-05-23 Compositions and methods for random nucleic acid mutagenisis

Publications (1)

Publication Number Publication Date
AT534731T true AT534731T (en) 2011-12-15

Family

ID=23132994

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02746439T AT534731T (en) 2001-05-30 2002-05-23 Compositions and methods for random mutagenesis of nucleic acids

Country Status (6)

Country Link
US (2) US6803216B2 (en)
EP (1) EP1390482B1 (en)
JP (1) JP2005501527A (en)
AT (1) AT534731T (en)
CA (1) CA2448628A1 (en)
WO (1) WO2002097078A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7507376B2 (en) * 2002-12-19 2009-03-24 3M Innovative Properties Company Integrated sample processing devices
EP1626978A4 (en) * 2003-02-06 2007-05-02 Rensselaer Polytech Inst Polymerase-based protocols for the introductions of deletions and insertions
FR2871170B1 (en) * 2004-06-07 2006-08-11 Proteus Sa A method for determining the mutational load of a gene bank obtained by random mutagenesis of a gene of interest and the means for its implementation
EP1989318B1 (en) * 2006-01-06 2014-07-30 Agilent Technologies, Inc. Reaction buffer composition for nucleic acid replication with packed dna polymerases
GB0620715D0 (en) 2006-10-18 2006-11-29 Univ Durham Ethanol production
GB201203374D0 (en) 2012-02-28 2012-04-11 Univ Sheffield Alkane production
CA2902189A1 (en) 2013-02-26 2014-09-04 Axiomx, Inc. Methods for the production of libraries for directed evolution
WO2017075421A1 (en) * 2015-10-29 2017-05-04 Temple University-Of The Commonwealth System Of Higher Education Modification of 3' terminal ends of nucleic acids by dna polymerase theta
GB201602535D0 (en) 2016-02-12 2016-03-30 Univ Edinburgh Improved flu vaccine yield

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
JP4245084B2 (en) * 1997-03-21 2009-03-25 ストラタジーン Polymerase enhancing factor (pef) extract, pef protein complexes isolated pef proteins, as well as purification and isolation methods
US6183997B1 (en) * 1997-03-21 2001-02-06 Stratagene Polymerase enhancing factor (PEF) extracts PEF protein complexes isolated PEF proteins and methods for purifying and identifying same
US20030049614A1 (en) * 1999-10-06 2003-03-13 Holly Hurlbut Hogrefe Compositions for dna amplification, synthesis, and mutagenesis
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure

Also Published As

Publication number Publication date
WO2002097078A1 (en) 2002-12-05
US6803216B2 (en) 2004-10-12
CA2448628A1 (en) 2002-12-05
EP1390482A1 (en) 2004-02-25
US20030152944A1 (en) 2003-08-14
EP1390482A4 (en) 2005-01-19
EP1390482B1 (en) 2011-11-23
JP2005501527A (en) 2005-01-20
US20050032177A1 (en) 2005-02-10

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