AT506449T - Direct rt-pcr on oligonucleotide immobilized pcr microplates - Google Patents

Direct rt-pcr on oligonucleotide immobilized pcr microplates

Info

Publication number
AT506449T
AT506449T AT07016364T AT07016364T AT506449T AT 506449 T AT506449 T AT 506449T AT 07016364 T AT07016364 T AT 07016364T AT 07016364 T AT07016364 T AT 07016364T AT 506449 T AT506449 T AT 506449T
Authority
AT
Austria
Prior art keywords
pcr
microplates
oligonucleotide immobilized
direct rt
rt
Prior art date
Application number
AT07016364T
Other languages
German (de)
Inventor
Masato Mitsuhashi
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Chemical Res Ct Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US6839497P priority Critical
Priority to US7162798P priority
Application filed by Hitachi Chemical Co Ltd, Hitachi Chemical Res Ct Inc filed Critical Hitachi Chemical Co Ltd
Publication of AT506449T publication Critical patent/AT506449T/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
AT07016364T 1997-12-22 1998-12-22 Direct rt-pcr on oligonucleotide immobilized pcr microplates AT506449T (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US6839497P true 1997-12-22 1997-12-22
US7162798P true 1998-01-16 1998-01-16

Publications (1)

Publication Number Publication Date
AT506449T true AT506449T (en) 2011-05-15

Family

ID=26748929

Family Applications (3)

Application Number Title Priority Date Filing Date
AT07016364T AT506449T (en) 1997-12-22 1998-12-22 Direct rt-pcr on oligonucleotide immobilized pcr microplates
AT98964860T AT401415T (en) 1997-12-22 1998-12-22 Direct RT-PCR on microtiter plates with immobilized oligonucleotide
AT05017491T AT397086T (en) 1997-12-22 1998-12-22 Cleaning mrna on microplates

Family Applications After (2)

Application Number Title Priority Date Filing Date
AT98964860T AT401415T (en) 1997-12-22 1998-12-22 Direct RT-PCR on microtiter plates with immobilized oligonucleotide
AT05017491T AT397086T (en) 1997-12-22 1998-12-22 Cleaning mrna on microplates

Country Status (5)

Country Link
EP (3) EP1865074B1 (en)
JP (3) JP4325114B2 (en)
AT (3) AT506449T (en)
DE (3) DE69839572D1 (en)
WO (1) WO1999032654A1 (en)

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US6686151B1 (en) 1998-02-06 2004-02-03 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US20050100943A1 (en) 2000-04-11 2005-05-12 Hideki Kambara Method of producing probe arrays for biological materials using fine particles
CN1245520C (en) * 1999-04-12 2006-03-15 日立化成工业株式会社 Method for producing probe arrays for biological materials using fine particles
FR2798675B1 (en) * 1999-09-16 2004-02-27 Centre Nat Rech Scient Method and device for manufacturing a support carriing a plurality of different polynucleotide and / or peptide sequences
CA2318730C (en) * 1999-09-20 2008-01-22 Ortho-Clinical Diagnostics, Inc. A method for reverse transcribing rna isolated in dry form
US6642000B1 (en) * 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
ITCA20000004A1 (en) * 2000-03-07 2001-09-07 Mario Raimondo Caria Method and system for the simultaneous and multiple detection with quantification of the hybridization of molecular compounds such as nucleic acids
US6376191B1 (en) * 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
DE10033091A1 (en) * 2000-07-07 2002-01-31 Max Planck Gesellschaft Polymer chip
CA2426655A1 (en) * 2000-09-27 2002-04-04 Michel Cabrera Method and device for making a support bearing a plurality of different polynucleotide and/or peptide sequences
US7374881B2 (en) 2000-10-31 2008-05-20 Hitachi Chemical Research Center, Inc. Method for collecting and using nuclear mRNA
US7285199B2 (en) 2000-10-31 2007-10-23 Hitachi Chemical Research Center, Inc. Apparatus and method for electrophoretic microspot concentration
GB0105790D0 (en) * 2001-03-08 2001-04-25 Expresson Biosystems Ltd Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
WO2003006650A1 (en) * 2001-07-09 2003-01-23 Asahi Kasei Kabushiki Kaisha Method of purifying nucleic acid using nonwoven fabric and detection method
JP4471659B2 (en) * 2002-01-09 2010-06-02 シスメックス株式会社 Direct nucleic acid amplification method
JP2005525114A (en) * 2002-04-24 2005-08-25 ヒタチ ケミカル リサーチ センター インコーポレイテッド Apparatus and method for quantifying mRNA from whole blood with high throughput
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
JP4480715B2 (en) 2003-01-29 2010-06-16 454 コーポレーション Double-end sequencing
CN1580283A (en) * 2003-08-13 2005-02-16 清华大学 Method for detecting nucleic acid molecule
DE10353887A1 (en) * 2003-11-18 2005-06-16 Febit Ag Highly parallel matrix-based DNA synthesizer
EP1548126A1 (en) * 2003-12-22 2005-06-29 Bio-Rad Pasteur Solid support for control nucleic acid, and application thereof to nucleic acid detection
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
EP1869615B1 (en) 2004-11-16 2010-03-03 Illumina, Inc. Methods and apparatus for reading coded microbeads
CA2594491A1 (en) * 2005-01-21 2006-08-24 The New York Blood Center, Inc. Method for extraction and identification of nucleic acids
US7816081B2 (en) 2005-04-28 2010-10-19 Hitachi Chemical Co., Ltd. Method of minimizing reagent consumption in microplate-based reactions
FR2885140B1 (en) * 2005-04-29 2010-12-31 Millipore Corp Method for detecting and characterizing microorgarists on a membrane
JP4916689B2 (en) * 2005-09-12 2012-04-18 住友ベークライト株式会社 DNA strand amplification method
US7830575B2 (en) 2006-04-10 2010-11-09 Illumina, Inc. Optical scanner with improved scan time
US8088580B2 (en) 2006-06-07 2012-01-03 Sumitomo Bakelite Company, Ltd. RNA detection method
BRPI0715346A2 (en) * 2006-08-01 2013-11-26 Applera Corp Analyte and nucleic acid detection
ES2622062T3 (en) 2008-10-27 2017-07-05 Qiagen Gaithersburg, Inc. Rapid results hybrid test and capture system
CA2760542A1 (en) 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
JP5729904B2 (en) * 2009-06-02 2015-06-03 キヤノン株式会社 Methods for preparing proteins, DNA, RNA from cells
JP5815518B2 (en) * 2009-07-17 2015-11-17 キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッドCanon U.S. Life Sciences, Inc. Method and system for isolating DNA in a microfluidic device
CA2773320C (en) 2009-09-14 2018-02-20 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
CA2787924A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
DK2556171T3 (en) 2010-04-05 2015-12-14 Prognosys Biosciences Inc Spatially CODED BIOLOGICAL ASSAYS
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
AU2011258501B2 (en) 2010-05-25 2016-07-07 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
AU2012220575B2 (en) 2011-02-24 2017-01-12 Qiagen Gaithersburg, Inc. Materials and methods for detection of HPV nucleic acid
CN104066849B (en) * 2011-10-11 2017-04-19 凯杰有限公司 Sample processing method and sample processing cartridge
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
JP2014176303A (en) * 2013-03-13 2014-09-25 Seiko Epson Corp Synthesizing method of cdna

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DE4034036C2 (en) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Apparatus and method for isolating nucleic acids from cell suspensions
JPH04187077A (en) * 1990-11-22 1992-07-03 Shimadzu Corp Apparatus for extraction and purification of nucleic acid
JPH04360686A (en) * 1991-06-04 1992-12-14 Tosoh Corp Purification of dna
US5437976A (en) * 1991-08-08 1995-08-01 Arizona Board Of Regents, The University Of Arizona Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
BR9206705A (en) * 1991-11-01 1995-11-21 Adelaide Children S Hospital The process of solid phase amplification
EP0675965A4 (en) * 1992-01-29 1999-01-20 Hitachi Chemical Co Ltd Polynucleotide immobilized support.
US5545528A (en) * 1993-04-15 1996-08-13 Hitachi Chemical Research Center Rapid screening method of gene amplification products in polypropylene plates
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5989431A (en) * 1995-06-08 1999-11-23 Progen Industries Ltd Method and apparatus for DNA extraction
DE69719409T2 (en) * 1996-06-18 2004-01-15 Rikagaku Kenkyusho Procedure for purifying DNA

Also Published As

Publication number Publication date
EP1865074A2 (en) 2007-12-12
JP2002505080A (en) 2002-02-19
AT401415T (en) 2008-08-15
AT397086T (en) 2008-06-15
JP4325114B2 (en) 2009-09-02
JP2006051042A (en) 2006-02-23
DE69842238D1 (en) 2011-06-01
JP4983836B2 (en) 2012-07-25
DE69839734D1 (en) 2008-08-28
EP1042497A4 (en) 2004-09-22
WO1999032654A1 (en) 1999-07-01
EP1600512A1 (en) 2005-11-30
DE69839572D1 (en) 2008-07-10
EP1865074A3 (en) 2008-02-27
EP1042497B1 (en) 2008-07-16
JP2009189368A (en) 2009-08-27
JP4337796B2 (en) 2009-09-30
EP1865074B1 (en) 2011-04-20
EP1600512B1 (en) 2008-05-28
EP1042497A1 (en) 2000-10-11

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