AT414767T - Nucleic acid-linker and its use in the gene synthesis - Google Patents

Nucleic acid-linker and its use in the gene synthesis

Info

Publication number
AT414767T
AT414767T AT01127864T AT01127864T AT414767T AT 414767 T AT414767 T AT 414767T AT 01127864 T AT01127864 T AT 01127864T AT 01127864 T AT01127864 T AT 01127864T AT 414767 T AT414767 T AT 414767T
Authority
AT
Austria
Prior art keywords
linker
use
nucleic acid
gene synthesis
gene
Prior art date
Application number
AT01127864T
Other languages
German (de)
Inventor
Octavian Schatz
Timothy O'connell
Original Assignee
Sloning Biotechnology Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sloning Biotechnology Gmbh filed Critical Sloning Biotechnology Gmbh
Priority to EP01127864A priority Critical patent/EP1314783B1/en
Publication of AT414767T publication Critical patent/AT414767T/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
AT01127864T 2001-11-22 2001-11-22 Nucleic acid-linker and its use in the gene synthesis AT414767T (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP01127864A EP1314783B1 (en) 2001-11-22 2001-11-22 Nucleic acid linkers and their use in gene synthesis

Publications (1)

Publication Number Publication Date
AT414767T true AT414767T (en) 2008-12-15

Family

ID=8179320

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01127864T AT414767T (en) 2001-11-22 2001-11-22 Nucleic acid-linker and its use in the gene synthesis

Country Status (8)

Country Link
US (1) US9957502B2 (en)
EP (1) EP1314783B1 (en)
AT (1) AT414767T (en)
AU (1) AU2002352108A1 (en)
CA (1) CA2468235C (en)
DE (1) DE50114507D1 (en)
DK (1) DK1314783T3 (en)
WO (1) WO2003044193A2 (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137906B2 (en) * 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
AT414767T (en) 2001-11-22 2008-12-15 Sloning Biotechnology Gmbh Nucleic acid-linker and its use in the gene synthesis
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
DK1557464T3 (en) * 2004-01-23 2011-01-24 Sloning Biotechnology Gmbh Enzymatic production of nykleinsyremolekylder
CN101213310A (en) * 2005-04-12 2008-07-02 Novia公司名下的现场Rcp公司 Methods for production of oligonucleotides
CN101238221B (en) 2005-04-12 2011-11-16 Novia公司名下的现场Rcp公司 Ring probe and its uses in identifying biomolecule
DE602007009634D1 (en) * 2006-01-04 2010-11-18 Si Lok Method for allocation of nucleic acids and identification finely textured variations in nucleic acids and auxiliaries for
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US9115352B2 (en) * 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
WO2011032181A2 (en) 2009-09-14 2011-03-17 Dyax Corp. Libraries of genetic packages comprising novel hc cdr3 designs
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
ES2548400T3 (en) * 2010-11-12 2015-10-16 Gen9, Inc. Methods and devices for nucleic acid synthesis
EP2737066B1 (en) * 2011-07-29 2017-11-08 Cellectis High throughput method for assembly and cloning polynucleotides comprising highly similar polynucleotidic modules
CN102584955A (en) * 2011-12-31 2012-07-18 北京唯尚立德生物科技有限公司 Solid-phase synthesis method of transcription activator-like effector
DE102012101347B4 (en) * 2012-02-20 2014-01-16 Markus Fuhrmann A method for preparing a nucleic acid library comprising at least two adjacent variable codon triplets
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013163263A2 (en) 2012-04-24 2013-10-31 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20140038240A1 (en) * 2012-07-10 2014-02-06 Pivot Bio, Inc. Methods for multipart, modular and scarless assembly of dna molecules
CA2918258A1 (en) 2013-08-05 2015-02-12 Twist Bioscience Corporation De novo synthesized gene libraries
US20170218416A1 (en) * 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna
US20170121716A1 (en) * 2014-07-03 2017-05-04 Rhodx, Inc. Tagging and assessing a target sequence
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
CA3006867A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
CA3034769A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4652639A (en) 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
IL82064D0 (en) * 1986-04-04 1987-10-20 Innogenetics Sa Nv Vectors comprising appropriate means for an enzymatical synthesis of nucleotide sequences,particularly dna fragments
US5093251A (en) 1986-05-23 1992-03-03 California Institute Of Technology Cassette method of gene synthesis
US5273879A (en) 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5132215A (en) 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
DK0786469T3 (en) 1990-06-11 2006-07-10 Gilead Sciences Inc Nucleic acid
AU672969B2 (en) 1992-03-10 1996-10-24 United States of America, as represented by The Secretary, Department of Health & Human Services, The Exchangeable template reaction
DE4343591A1 (en) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh A method for evolutionary design and synthesis of functional polymers on the basis of mold elements and shape codes
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5770365A (en) 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
GB9618544D0 (en) 1996-09-05 1996-10-16 Brax Genomics Ltd Characterising DNA
US6110668A (en) 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
US5981190A (en) 1997-01-08 1999-11-09 Ontogeny, Inc. Analysis of gene expression, methods and reagents therefor
US5888737A (en) 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
DE19736591A1 (en) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Preparing long nucleic acid polymers from linkable oligonucleotides
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
DE19812103A1 (en) * 1998-03-19 1999-09-23 Bernauer Annette Iteratively synthesizing nucleic acid from oligonucleotides which contain cleavable sites, useful for preparation of genes, ribozymes etc.
DE19925862A1 (en) * 1999-06-07 2000-12-14 Diavir Gmbh Process for the synthesis of DNA fragments
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
AU3214001A (en) * 2000-02-17 2001-08-27 Complete Genomics As A method of mapping restriction endonuclease cleavage sites
US6468749B1 (en) * 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
AT414767T (en) 2001-11-22 2008-12-15 Sloning Biotechnology Gmbh Nucleic acid-linker and its use in the gene synthesis
US20030219781A1 (en) 2002-01-14 2003-11-27 Diversa Corporation Compositions and methods for making polynucleotides by iterative assembly of codon building blocks
DE60227525D1 (en) 2002-10-18 2008-08-21 Sloning Biotechnology Gmbh A process for the manufacture of nucleic acid molecules
DK1557464T3 (en) * 2004-01-23 2011-01-24 Sloning Biotechnology Gmbh Enzymatic production of nykleinsyremolekylder

Also Published As

Publication number Publication date
EP1314783A1 (en) 2003-05-28
WO2003044193A2 (en) 2003-05-30
DK1314783T3 (en) 2009-03-16
US20080044862A1 (en) 2008-02-21
DE50114507D1 (en) 2009-01-02
AU2002352108A8 (en) 2003-06-10
CA2468235C (en) 2013-05-28
CA2468235A1 (en) 2003-05-30
WO2003044193A3 (en) 2004-04-08
US9957502B2 (en) 2018-05-01
AU2002352108A1 (en) 2003-06-10
EP1314783B1 (en) 2008-11-19

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