AT362532T - Method for the amplification of a nucleic acid sequence using a chimeric primer - Google Patents

Method for the amplification of a nucleic acid sequence using a chimeric primer


Publication number
AT362532T AT00908056T AT00908056T AT362532T AT 362532 T AT362532 T AT 362532T AT 00908056 T AT00908056 T AT 00908056T AT 00908056 T AT00908056 T AT 00908056T AT 362532 T AT362532 T AT 362532T
Prior art keywords
nucleic acid
acid sequence
chimeric primer
Prior art date
Application number
Other languages
German (de)
Hiroyuki Mukai
Junko Yamamoto
Osamu Takeda
Kazue Miyake
Takashi Uemori
Yoshimi Sato
Mariko Moriyama
Haruhisa Sawaragi
Michio Hagiya
Kiyozo Asada
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP7696699 priority Critical
Priority to JP37003599 priority
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Publication of AT362532T publication Critical patent/AT362532T/en



    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
AT00908056T 1999-03-19 2000-03-14 Method for the amplification of a nucleic acid sequence using a chimeric primer AT362532T (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP7696699 1999-03-19
JP37003599 1999-12-27

Publications (1)

Publication Number Publication Date
AT362532T true AT362532T (en) 2007-06-15



Family Applications (1)

Application Number Title Priority Date Filing Date
AT00908056T AT362532T (en) 1999-03-19 2000-03-14 Method for the amplification of a nucleic acid sequence using a chimeric primer

Country Status (10)

Country Link
EP (1) EP1167524B1 (en)
JP (1) JP3433929B2 (en)
CN (1) CN1227357C (en)
AT (1) AT362532T (en)
AU (1) AU773536B2 (en)
CA (1) CA2365135C (en)
DE (1) DE60034878T2 (en)
EA (2) EA005577B1 (en)
TW (1) TWI259204B (en)
WO (1) WO2000056877A1 (en)

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AU2001286268B2 (en) * 2000-09-19 2006-04-27 Takara Bio Inc. Method of forming complex
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KR20030064420A (en) * 2000-12-26 2003-07-31 다카라 바이오 가부시키가이샤 Method of detecting pathogenic microorganism
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EA005141B1 (en) 2001-02-15 2004-12-30 Такара Био Инк. A method for detecting nucleotide polymorphism
JP2002253257A (en) * 2001-03-02 2002-09-10 Tosoh Corp Oligonucleotide for vero toxin detection and method for detection
NZ523167A (en) 2001-03-09 2006-08-31 Nugen Technologies Inc Methods, compositions and kits for amplifying RNA sequences of interest which employ an RNA/DNA composite primer
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
AU2003303395A1 (en) 2003-12-23 2004-07-22 Dahl, Gary, A. Target-dependent transcription using deletion mutants of n4 rna polymerase
WO2002101041A1 (en) * 2001-06-12 2002-12-19 Takara Bio Inc. Method of amplifying nucleic acid and method of detecting nucleotide polymorphism with the use of nucleotide analog
JPWO2002101042A1 (en) * 2001-06-12 2005-04-07 タカラバイオ株式会社 Stabilizing method and storage method of nucleic acid amplification or detection reactions reagent
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JP2005102502A (en) * 2001-11-21 2005-04-21 Wakunaga Pharmaceut Co Ltd Method for amplifying single-stranded nucleic acid fragment
AU2003211421A1 (en) * 2002-03-07 2003-09-16 Takara Bio Inc. Method of detecting base substitution
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CN1738914A (en) * 2002-11-21 2006-02-22 震源技术公司 Methods for using primers that encode one strand of a double-stranded promoter
EP1613778B1 (en) 2003-04-14 2014-11-26 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US20050239087A1 (en) * 2003-04-29 2005-10-27 Haiguang Xiao Multiply-primed amplification of nucleic acid sequences
EP1659170A1 (en) * 2003-07-11 2006-05-24 Taiyo Yuden Co., Ltd. Nucleic acid amplifier and method of nucleic acid amplification
JP4249186B2 (en) 2003-12-10 2009-04-02 タカラバイオ株式会社 Nucleic acid amplification method
JP4548174B2 (en) 2005-03-24 2010-09-22 コニカミノルタエムジー株式会社 Microchip for inspection and inspection apparatus using the same
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CN101203618B (en) 2005-05-26 2013-03-13 人类遗传标记控股有限公司 Isothermal strand displacement amplification using primers containing a non-regular base
CA2621267A1 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US8133456B2 (en) 2005-11-07 2012-03-13 Konica Minolta Medical & Graphic, Inc. Microreactor and method of liquid feeding making use of the same
JP2009219355A (en) * 2006-07-05 2009-10-01 Synthera Technologies Co Ltd Method of detecting target substance
JP4918409B2 (en) 2006-07-26 2012-04-18 西川ゴム工業株式会社 Nucleic acid sequence amplification method
US8202972B2 (en) 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
JP4340298B2 (en) 2007-03-01 2009-10-07 株式会社日立ハイテクノロジーズ Nucleic acid recovery method and nucleic acid recovery apparatus
WO2009042291A2 (en) * 2007-08-03 2009-04-02 Igor Kutyavin Accelerated cascade amplification (aca) of nucleic acids comprising strand and sequence specific dna nicking
WO2009044773A1 (en) 2007-10-04 2009-04-09 Tosoh Corporation Primer for amplification of rrna of bacterium belonging to the genus legionella, detection method, and detection kit
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
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US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
ES2637843T3 (en) 2008-10-24 2017-10-17 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
JPWO2010061922A1 (en) 2008-11-27 2012-04-26 独立行政法人理化学研究所 Novel MutS protein and method for determining mutation using the same
US20110269194A1 (en) * 2010-04-20 2011-11-03 Swift Biosciences, Inc. Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
JP5454338B2 (en) 2010-04-28 2014-03-26 株式会社島津製作所 Real-time nucleic acid amplification by droplet manipulation
CN105772122B (en) 2010-12-21 2017-11-03 株式会社岛津制作所 Apparatus and method for for the operation object composition in pipe
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US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
US10253360B2 (en) 2012-09-28 2019-04-09 Bna Inc. BNA clamp method
US20150299772A1 (en) * 2012-12-03 2015-10-22 Elim Biopharmaceuticals, Inc. Single-stranded polynucleotide amplification methods
US20150275285A1 (en) * 2012-12-03 2015-10-01 Yilin Zhang Compositions and methods of nucleic acid preparation and analyses
KR101589483B1 (en) * 2014-02-21 2016-01-28 디엑스진주식회사 Method for Detection of Nucleic Acids by Asymmetric Isothermal Amplification of Nucleic Acids and Signal Probe
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Also Published As

Publication number Publication date
CA2365135C (en) 2009-08-18
EA004327B1 (en) 2004-04-29
AU2944200A (en) 2000-10-09
TWI259204B (en) 2006-08-01
DE60034878T2 (en) 2008-01-17
EP1167524B1 (en) 2007-05-16
CN1227357C (en) 2005-11-16
EA200100990A1 (en) 2002-02-28
JP3433929B2 (en) 2003-08-04
EA005577B1 (en) 2005-04-28
CN1350581A (en) 2002-05-22
AU773536B2 (en) 2004-05-27
WO2000056877A1 (en) 2000-09-28
EP1167524A4 (en) 2005-03-09
DE60034878D1 (en) 2007-06-28
EP1167524A1 (en) 2002-01-02
CA2365135A1 (en) 2000-09-28
EA200300991A1 (en) 2004-02-26

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