AT282693T - Method for producing insertion mutants - Google Patents

Method for producing insertion mutants

Info

Publication number
AT282693T
AT282693T AT99969437T AT99969437T AT282693T AT 282693 T AT282693 T AT 282693T AT 99969437 T AT99969437 T AT 99969437T AT 99969437 T AT99969437 T AT 99969437T AT 282693 T AT282693 T AT 282693T
Authority
AT
Austria
Prior art keywords
method
insertion mutants
producing insertion
producing
mutants
Prior art date
Application number
AT99969437T
Other languages
German (de)
Inventor
William S Reznikoff
Igor Y Goryshin
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US09/159,363 priority Critical patent/US6159736A/en
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Priority to PCT/US1999/021960 priority patent/WO2000017343A1/en
Publication of AT282693T publication Critical patent/AT282693T/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
AT99969437T 1998-09-23 1999-09-21 Method for producing insertion mutants AT282693T (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US09/159,363 US6159736A (en) 1998-09-23 1998-09-23 Method for making insertional mutations using a Tn5 synaptic complex
PCT/US1999/021960 WO2000017343A1 (en) 1998-09-23 1999-09-21 Method for making insertional mutations

Publications (1)

Publication Number Publication Date
AT282693T true AT282693T (en) 2004-12-15

Family

ID=22572286

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99969437T AT282693T (en) 1998-09-23 1999-09-21 Method for producing insertion mutants

Country Status (12)

Country Link
US (2) US6159736A (en)
EP (1) EP1115856B1 (en)
JP (4) JP4464563B2 (en)
CN (1) CN100420747C (en)
AT (1) AT282693T (en)
AU (1) AU758960B2 (en)
CA (1) CA2343000C (en)
DE (1) DE69922032T2 (en)
ES (1) ES2228170T3 (en)
PL (1) PL197408B1 (en)
RU (1) RU2237715C2 (en)
WO (1) WO2000017343A1 (en)

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US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
US6406896B1 (en) * 1999-08-02 2002-06-18 Wisconsin Alumni Research Foundation Transposase enzyme and method for use
EP1268837B1 (en) * 2000-03-21 2009-07-15 Minos Biosystems Limited Method of generating transgenic mammals using transposons
WO2002004629A2 (en) * 2000-07-07 2002-01-17 Maxygen, Inc. Molecular breeding of transposable elements
WO2002013610A1 (en) * 2000-08-17 2002-02-21 Mirus Corporation Nucleic acid expression from linear nucleic acids
US7067644B2 (en) 2000-12-05 2006-06-27 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
US7138267B1 (en) * 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
US20030143740A1 (en) * 2001-10-15 2003-07-31 Christine Wooddell Processes for transposase mediated integration into mammalian cells
US7262056B2 (en) * 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
US7101713B1 (en) * 2002-05-16 2006-09-05 Epicentre Technologies Corporation DNA transformation efficiency by inhibiting host cell restriction
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20040172667A1 (en) 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
FI20030561A0 (en) 2003-04-14 2003-04-14 Finnzymes Oy A method for transferring nucleic acids into eukaryotic genomes
WO2004093645A2 (en) * 2003-04-17 2004-11-04 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
WO2005062881A2 (en) 2003-12-24 2005-07-14 Transgenrx, Inc. Gene therapy using transposon-based vectors
US7727744B2 (en) * 2004-03-30 2010-06-01 Epicentre Technologies Corporation Methods for obtaining directionally truncated polypeptides
US7608434B2 (en) * 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
EP1752532A1 (en) 2005-08-09 2007-02-14 Helmholtz-Zentrum für Infektionsforschung GmbH Extracellular polyhydroxyalkanoates produced by genetically engineered microorganisms
WO2010036976A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
DK2963709T3 (en) 2008-10-24 2017-09-11 Epicentre Tech Corp Transposon-end compositions and procedures for modification of nucleic acids
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9150881B2 (en) 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2954065A4 (en) 2013-02-08 2016-08-24 10X Genomics Inc Partitioning and processing of analytes and other species
SG11201508985VA (en) 2013-05-23 2015-12-30 Univ Leland Stanford Junior Transposition into native chromatin for personal epigenomics
AU2014284560A1 (en) 2013-07-03 2015-12-24 Illumina, Inc. Sequencing by orthogonal synthesis
CN110548550A (en) 2014-04-10 2019-12-10 10X基因组学有限公司 Fluidic devices, systems and methods for packaging and partitioning reagents and uses thereof
JP6490710B2 (en) * 2014-04-15 2019-03-27 イラミーナ インコーポレーテッド Modified transposase for improved insert bias and expanded DNA input tolerance
WO2015179706A1 (en) 2014-05-23 2015-11-26 Fluidigm Corporation Haploidome determination by digitized transposons
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2016061098A1 (en) * 2014-10-14 2016-04-21 Covaris, Inc. Acoustic energy mediation of genetic fragmentation
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
JP2018500921A (en) 2015-01-12 2018-01-18 10エックス ジェノミクス, インコーポレイテッド Processes and systems for making nucleic acid sequencing libraries, and libraries made using them
WO2016180379A1 (en) * 2015-05-14 2016-11-17 Universidad De Los Andes Novel transposon that promotes the functional expression of genes in episomal dna, and method for increasing dna transcription in the functional analysis of metagenomic libraries
CN107771223A (en) 2015-07-30 2018-03-06 亿明达股份有限公司 The orthogonal deblocking of nucleotides
CA2995305A1 (en) 2015-08-12 2017-02-16 Cemm Forschungszentrum Fur Molekulare Medizin Gmbh Methods for studying nucleic acids
US20190169602A1 (en) * 2016-01-12 2019-06-06 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
ES2634802B1 (en) * 2016-03-28 2018-07-03 Victor Manuel GALVEZ JEREZ Nucleic acid molecule and method for biallelic modification of a target gene or locus present in the genetic material of a cell
CN106222164A (en) * 2016-07-28 2016-12-14 元码基因科技(北京)有限公司 Transposon enzyme is utilized to carry out the method for the unidirectional amplification of external nucleic acid, compositions and test kit
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10428326B2 (en) 2017-01-30 2019-10-01 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
AU2018261332A1 (en) 2017-05-01 2019-11-07 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
SG11201910070PA (en) 2017-05-08 2019-11-28 Illumina Inc Universal short adapters for indexing of polynucleotide samples
US20190085384A1 (en) 2017-09-15 2019-03-21 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US132349A (en) * 1872-10-22 Improvement in fish-ways
US5639949A (en) * 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US5965443A (en) * 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5925545A (en) * 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
JP2003505104A (en) * 1999-08-02 2003-02-12 ウイスコンシン アラムニ リサーチ ファンデーション Mutant tn5 transposase enzymes and methods of use thereof

Also Published As

Publication number Publication date
CN1319135A (en) 2001-10-24
PL346772A1 (en) 2002-02-25
JP5961138B2 (en) 2016-08-02
CN100420747C (en) 2008-09-24
AU758960B2 (en) 2003-04-03
JP2013165731A (en) 2013-08-29
WO2000017343A1 (en) 2000-03-30
DE69922032D1 (en) 2004-12-23
RU2237715C2 (en) 2004-10-10
PL197408B1 (en) 2008-03-31
CA2343000C (en) 2005-12-20
EP1115856B1 (en) 2004-11-17
AU6057399A (en) 2000-04-10
US6159736A (en) 2000-12-12
JP2016119917A (en) 2016-07-07
JP2010068813A (en) 2010-04-02
JP2002531062A (en) 2002-09-24
JP4464563B2 (en) 2010-05-19
CA2343000A1 (en) 2000-03-30
US6294385B1 (en) 2001-09-25
ES2228170T3 (en) 2005-04-01
EP1115856A1 (en) 2001-07-18
JP5937777B2 (en) 2016-06-22
DE69922032T2 (en) 2005-12-01

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