AT205879T - A method for cloning of mRNA and representation of differentially expressed transcripts (Dodet) - Google Patents

A method for cloning of mRNA and representation of differentially expressed transcripts (Dodet)

Info

Publication number
AT205879T
AT205879T AT98919092T AT98919092T AT205879T AT 205879 T AT205879 T AT 205879T AT 98919092 T AT98919092 T AT 98919092T AT 98919092 T AT98919092 T AT 98919092T AT 205879 T AT205879 T AT 205879T
Authority
AT
Austria
Prior art keywords
dodet
mrna
cloning
representation
method
Prior art date
Application number
AT98919092T
Other languages
German (de)
Inventor
Peter Rolf Warthoe
Original Assignee
Azign Bioscience As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DK54797 priority Critical
Priority to US87103097A priority
Priority to DK43298 priority
Application filed by Azign Bioscience As filed Critical Azign Bioscience As
Priority to PCT/DK1998/000186 priority patent/WO1998051789A2/en
Publication of AT205879T publication Critical patent/AT205879T/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16CCOMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/60In silico combinatorial chemistry
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
AT98919092T 1997-05-13 1998-05-13 A method for cloning of mRNA and representation of differentially expressed transcripts (Dodet) AT205879T (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DK54797 1997-05-13
US87103097A true 1997-05-19 1997-05-19
DK43298 1998-03-27
PCT/DK1998/000186 WO1998051789A2 (en) 1997-05-13 1998-05-13 A METHOD TO CLONE mRNAs AND DISPLAY OF DIFFERENTIALLY EXPRESSED TRANSCRIPTS (DODET)

Publications (1)

Publication Number Publication Date
AT205879T true AT205879T (en) 2001-10-15

Family

ID=27220672

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98919092T AT205879T (en) 1997-05-13 1998-05-13 A method for cloning of mRNA and representation of differentially expressed transcripts (Dodet)

Country Status (9)

Country Link
EP (2) EP1138764A3 (en)
JP (1) JP2001515362A (en)
AT (1) AT205879T (en)
AU (1) AU7206498A (en)
DE (1) DE69801749T2 (en)
DK (1) DK0981609T3 (en)
ES (1) ES2163271T3 (en)
PT (1) PT981609E (en)
WO (1) WO1998051789A2 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2287542A1 (en) 1997-04-22 1998-10-29 Life Technologies, Inc. Methods for the production of aslv reverse transcriptases composed of multiple subunits
US20020090612A1 (en) * 1999-01-08 2002-07-11 Jonathan M. Rothberg Method of identifying nucleic acids
SG89289A1 (en) * 1999-08-14 2002-06-18 Kent Ridge Digital Labs Classification by aggregating emerging patterns
US6468749B1 (en) 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
US6387624B1 (en) * 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
CA2413423C (en) 2000-06-30 2011-01-11 Syngenta Participations Ag Method for identification, separation and quantitative measurement of nucleic acid fragments
US6706476B1 (en) * 2000-08-22 2004-03-16 Azign Bioscience A/S Process for amplifying and labeling single stranded cDNA by 5′ ligated adaptor mediated amplification
EP1325118B1 (en) 2000-10-05 2016-05-25 Riken Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers.
AU2002222618B2 (en) * 2000-12-12 2005-06-30 Masumi Abe Method of analyzing gene expression
EP1356118A2 (en) 2001-01-24 2003-10-29 Syngenta Participations AG Method for non-redundant library construction
EP1434883A2 (en) * 2001-10-02 2004-07-07 Azign Bioscience A/S Specific differential display arrays
AT358182T (en) * 2002-09-05 2007-04-15 Plant Bioscience Ltd Genome division
EP2145955B1 (en) * 2003-01-29 2012-02-22 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
US7498135B2 (en) 2003-06-19 2009-03-03 National Institute Of Radiological Sciences Method for preparing gene expression profile
JP4217780B2 (en) * 2003-06-19 2009-02-04 独立行政法人放射線医学総合研究所 Gene expression profile creation method
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
JP4879975B2 (en) * 2005-05-31 2012-02-22 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー Multiplex amplification of short nucleic acids
CN101278058A (en) * 2005-06-23 2008-10-01 科因股份有限公司 Improved strategies for sequencing complex genomes using high throughput sequencing technologies
JP5220597B2 (en) 2005-06-23 2013-06-26 キージーン ナムローゼ フェンノートシャップ Method for identifying one or more polymorphisms and methods of use thereof
WO2007025281A2 (en) 2005-08-24 2007-03-01 Applera Corporation A method to quantify sirnas, mirnas and polymorphic mirnas
CA2623539C (en) 2005-09-29 2015-12-15 Keygene N.V. High throughput screening of mutagenized populations
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
EP2363504A1 (en) 2005-12-22 2011-09-07 Keygene N.V. Method for high-throughtput AFLP-based polymorphism detection
WO2007114693A2 (en) 2006-04-04 2007-10-11 Keygene N.V. High throughput detection of molecular markers based on aflp and high throughput sequencing
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
US10385392B2 (en) * 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2100945T3 (en) * 1989-12-22 1997-07-01 Hoffmann La Roche High temperature reverse transcriptases.
EP0592626B1 (en) * 1992-03-11 2003-01-29 Dana-Farber Cancer Institute, Inc. METHODS TO CLONE mRNA
US5482845A (en) * 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5459037A (en) * 1993-11-12 1995-10-17 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
DE19518505A1 (en) * 1995-05-19 1996-11-21 Max Planck Gesellschaft A method for gene expression analysis
US5712126A (en) * 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
AU2264197A (en) * 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas
US5830714A (en) * 1996-04-17 1998-11-03 Molecular Biology Resources, Inc. Biologically active fragment of bacillus stearothermophilus DNA polymerase

Also Published As

Publication number Publication date
DK981609T3 (en)
DE69801749T2 (en) 2002-07-04
ES2163271T3 (en) 2002-01-16
JP2001515362A (en) 2001-09-18
PT981609E (en) 2002-03-28
AU7206498A (en) 1998-12-08
EP0981609A2 (en) 2000-03-01
WO1998051789A3 (en) 1999-03-18
EP0981609B1 (en) 2001-09-19
EP1138764A3 (en) 2001-10-24
DE69801749D1 (en) 2001-10-25
WO1998051789A9 (en) 1999-12-09
EP1138764A2 (en) 2001-10-04
WO1998051789A2 (en) 1998-11-19
DK0981609T3 (en) 2001-11-12

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee