AT151111T - Recombinant dna method and products produced by this - Google Patents

Recombinant dna method and products produced by this

Info

Publication number
AT151111T
AT151111T AT85306957T AT85306957T AT151111T AT 151111 T AT151111 T AT 151111T AT 85306957 T AT85306957 T AT 85306957T AT 85306957 T AT85306957 T AT 85306957T AT 151111 T AT151111 T AT 151111T
Authority
AT
Austria
Prior art keywords
recombinant dna
products produced
products
produced
recombinant
Prior art date
Application number
AT85306957T
Other languages
German (de)
Inventor
Cha-Mer Wei
Nancy Hsiung
Vermuri B Reddy
Jeffrey F Lemontt
William Dackowski
Richard Douglas
Edward S Cole
Richard D Purcell Jr
David Tai-Yui Lau
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US65677084A priority Critical
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of AT151111T publication Critical patent/AT151111T/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6456Plasminogen activators
    • C12N9/6459Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21069Protein C activated (3.4.21.69)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00Bacteriophages
    • C12N2795/00011Bacteriophages
    • C12N2795/14011Bacteriophages ssDNA Bacteriophages
    • C12N2795/14111Inoviridae
    • C12N2795/14141Use of virus, viral particle or viral elements as a vector
    • C12N2795/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/001Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/70Vector systems having a special element relevant for transcription from fungi
    • C12N2830/702Vector systems having a special element relevant for transcription from fungi yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/80Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85Vector systems having a special element relevant for transcription from vertebrates mammalian
AT85306957T 1984-10-01 1985-09-30 Recombinant dna method and products produced by this AT151111T (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US65677084A true 1984-10-01 1984-10-01

Publications (1)

Publication Number Publication Date
AT151111T true AT151111T (en) 1997-04-15

Family

ID=24634490

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85306957T AT151111T (en) 1984-10-01 1985-09-30 Recombinant dna method and products produced by this

Country Status (14)

Country Link
US (1) US5344773A (en)
EP (1) EP0178105B1 (en)
JP (1) JPS61149094A (en)
AT (1) AT151111T (en)
AU (1) AU599576B2 (en)
DE (1) DE3588149T2 (en)
DK (1) DK175327B1 (en)
ES (3) ES8702945A1 (en)
FI (1) FI89723C (en)
IE (1) IE81135B1 (en)
NO (1) NO175158C (en)
NZ (1) NZ213647A (en)
PT (1) PT81231B (en)
ZA (1) ZA8507574B (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0143081B1 (en) * 1983-11-21 1989-08-23 Ciba-Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast
US5073494A (en) * 1985-04-22 1991-12-17 Genentech, Inc. Human tissue plasminogen activator substituted at position 275 or at positions 275 and 277
US5047241A (en) * 1986-07-11 1991-09-10 American Home Products Corporation Tris-Kringle plasminogen activator with novel K2 insert
GB8528321D0 (en) * 1985-11-18 1985-12-24 Ciba Geigy Ag Modified fibrinolytic agents
JP2581668B2 (en) * 1985-11-27 1997-02-12 三井東圧化学株式会社 Novel DNA sequence encoding human tissue plasminogen activator derived from normal human cells and vectors and cells containing the same
US5501853A (en) * 1985-12-23 1996-03-26 Chiron Corporation Peptide plasminogen activators
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
DE3789664T3 (en) * 1986-01-31 2004-09-16 Genetics Institute, LLC, Cambridge NEW THROMBOLYTIC PROTEINS.
US5589361A (en) * 1986-03-18 1996-12-31 Genentech, Inc. Human tissue-type plasminogen activator variant
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
JP2874751B2 (en) 1986-04-09 1999-03-24 ジェンザイム・コーポレーション Transgenic animals secreting the desired protein into milk
EP0275282A1 (en) * 1986-07-16 1988-07-27 Celltech Limited Process for purifying a plasminogen activator
US5580559A (en) * 1986-12-05 1996-12-03 Ciba-Geigy Corporation Hybrid plasminogen activator
US5242819A (en) * 1986-12-05 1993-09-07 Ciba-Geigy Corporation DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase
EP0276846A3 (en) * 1987-01-29 1989-07-26 Zymogenetics, Inc. Colony-stimulating factor derivatives
JP2983997B2 (en) * 1987-01-30 1999-11-29 アメリカン・ホーム・プロダクツ・コーポレイション De-epidermal growth factor plasminogen activator
JPH084501B2 (en) * 1987-03-20 1996-01-24 エーザイ株式会社 Mutant tPA related to sugar chain
NO882226L (en) * 1987-05-22 1988-11-23 Zymogenetics Inc FIBRINOLYTIC PROTEINS.
AU1668988A (en) * 1987-06-03 1988-12-08 Smithkline Beckman Corporation Purification of tpa
US6682733B1 (en) * 1987-06-18 2004-01-27 Roche Diagnostics, Gmbh Fibrinolytic enzymes
DE3726019A1 (en) * 1987-08-05 1989-02-16 Boehringer Mannheim Gmbh METHOD FOR SEPARATING AND PURIFYING T-PA
US5100666A (en) * 1987-10-09 1992-03-31 Monsanto Company Modified tissue plasminogen activator K2K2SP
US5244676A (en) * 1987-10-09 1993-09-14 Monsanto Company Modified tissue plasminogen activator with modified glycosylation site
US4963357A (en) * 1987-10-09 1990-10-16 Monsanto Company Tissue plasminogen activator modified by the substitution of Arg for Cys at position 73, methods and pharmaceutical compositions therefor
US5037752A (en) * 1987-10-09 1991-08-06 Monsanto Company Modified tissue plasminogen activator substituted at cysteine-73 and lysine-277
US5270198A (en) * 1988-05-20 1993-12-14 Genentech, Inc. DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells
US5665583A (en) * 1988-08-12 1997-09-09 Arch Dev Corp Methods and materials relating to IMPDH and GMP production
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5070021A (en) * 1989-01-10 1991-12-03 Monsanto Company Method of modifying oligosaccharide structure of tissue plasminogen activator
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
CA2129660C (en) * 1992-06-03 2005-06-28 William F. Bennett Tissue plasminogen activator glycosylation variants with improved therapeutic properties
US7299805B2 (en) 2002-06-07 2007-11-27 Marctec, Llc Scaffold and method for implanting cells
WO2014116706A1 (en) * 2013-01-22 2014-07-31 University Of Tennessee Research Foundation Tissue plasminogen activator antibodies and methods of use thereof

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4419446A (en) * 1980-12-31 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Recombinant DNA process utilizing a papilloma virus DNA as a vector
KR840004781A (en) * 1982-05-05 1984-10-24 월터 에이치·드레거 Human tissue plasminogen activator
GR79202B (en) * 1982-05-05 1984-10-22 Genentech Inc
WO1984001786A1 (en) * 1982-10-28 1984-05-10 Beecham Group Plc Enzyme derivatives and their use in the treatment of thrombosis
AU554862B2 (en) * 1982-12-14 1986-09-04 Implico B.V. Plasminogen activator isolated by affinity chromatography
IE56716B1 (en) * 1983-01-19 1991-11-20 Genentech Inc Method for producing human tpa and expression vectors therefor
EP0143081B1 (en) * 1983-11-21 1989-08-23 Ciba-Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
GB8528321D0 (en) * 1985-11-18 1985-12-24 Ciba Geigy Ag Modified fibrinolytic agents
US5501853A (en) * 1985-12-23 1996-03-26 Chiron Corporation Peptide plasminogen activators
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation

Also Published As

Publication number Publication date
FI89723B (en) 1993-07-30
AU599576B2 (en) 1990-07-26
DE3588149T2 (en) 1997-10-09
FI853761A (en)
PT81231A (en) 1985-11-01
DE3588149D1 (en) 1997-05-07
ES8706726A1 (en) 1987-07-01
ES547416A0 (en) 1987-01-16
EP0178105A2 (en) 1986-04-16
FI853761L (en) 1986-04-02
ES8801306A1 (en) 1988-01-01
ES554450A0 (en) 1988-01-01
DK175327B1 (en) 2004-08-23
NO175158B (en) 1994-05-30
FI853761A0 (en) 1985-09-30
ES554449D0 (en)
EP0178105B1 (en) 1997-04-02
PT81231B (en) 1987-10-20
JPS61149094A (en) 1986-07-07
ES8702945A1 (en) 1987-01-16
ZA8507574B (en) 1986-06-25
DK441585A (en) 1986-04-02
NZ213647A (en) 1989-05-29
DK441585D0 (en) 1985-09-30
AU4814885A (en) 1986-04-10
EP0178105A3 (en) 1988-11-17
NO175158C (en) 1994-09-07
FI89723C (en) 1993-11-10
US5344773A (en) 1994-09-06
IE852394L (en) 1986-04-01
ES554450D0 (en)
IE81135B1 (en) 2000-03-22
FI853761D0 (en)
ES554449A0 (en) 1987-07-01
ES547416D0 (en)
NO853851L (en) 1986-04-02

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time