AR091298A1 - MANIPULATED LEAVES OF YEAST TO PRODUCE ETHANOL FROM ACETIC ACID AND GLYCEROL - Google Patents
MANIPULATED LEAVES OF YEAST TO PRODUCE ETHANOL FROM ACETIC ACID AND GLYCEROLInfo
- Publication number
- AR091298A1 AR091298A1 ARP120104522A AR091298A1 AR 091298 A1 AR091298 A1 AR 091298A1 AR P120104522 A ARP120104522 A AR P120104522A AR 091298 A1 AR091298 A1 AR 091298A1
- Authority
- AR
- Argentina
- Prior art keywords
- glycerol
- acetic acid
- cell
- yeast
- yeast cells
- Prior art date
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Procesos para producir etanol a partir de hidrolizados lignocelulósicos que comprenden hexosas, pentosas y ácido acético, en el cual se usan células de levadura modificadas genéticamente que comprenden un gen exógeno que codifica una acetaldehído deshidrogenasa y un gen bacteriano que codifica una enzima con actividad glicerol deshidrogenasa ligada a NAD⁺. El proceso se caracteriza además porque el glicerol está presente o es alimentado al medio de cultivo, con lo cual la célula de levadura modificada fermenta las hexosas, las pentosas, el ácido acético y el glicerol en etanol. La presente se relaciona además con células de levadura que se usan en dichos procesos. Las células de levadura comprenden ventajosamente modificaciones genéticas que mejoran la utilización de glicerol, tales como modificaciones que aumentan una o más entre actividad dihidroxiacetona quinasa y el transporte de glicerol dentro de la célula. Preferiblemente la célula de levadura comprende además un gen de xilosa isomerasa exógeno funcional y/o genes exógenos funcionales que le confieren a la célula la capacidad para convertir L-arabinosa en D-xilulosa 5-fosfato y pueden comprender una modificación genética que aumenta la actividad acetil-CoA sintetasa.Processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, in which genetically modified yeast cells comprising an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with glycerol dehydrogenase activity are used linked to NAD⁺. The process is further characterized in that glycerol is present or is fed to the culture medium, whereby the modified yeast cell ferments hexoses, pentoses, acetic acid and glycerol in ethanol. This is also related to yeast cells that are used in said processes. Yeast cells advantageously comprise genetic modifications that improve the use of glycerol, such as modifications that increase one or more between dihydroxyacetone kinase activity and the transport of glycerol within the cell. Preferably the yeast cell further comprises a functional exogenous xylose isomerase gene and / or functional exogenous genes that give the cell the ability to convert L-arabinose into D-xylulose 5-phosphate and may comprise a genetic modification that increases activity acetyl-CoA synthetase.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11191333 | 2011-11-30 | ||
| EP61564932 | 2011-11-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR091298A1 true AR091298A1 (en) | 2015-01-28 |
Family
ID=52623091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120104522 AR091298A1 (en) | 2011-11-30 | 2012-11-30 | MANIPULATED LEAVES OF YEAST TO PRODUCE ETHANOL FROM ACETIC ACID AND GLYCEROL |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR091298A1 (en) |
-
2012
- 2012-11-30 AR ARP120104522 patent/AR091298A1/en unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |