AR084844A1 - Arboles de citricos con resistencia a cancro de los citricos - Google Patents

Arboles de citricos con resistencia a cancro de los citricos

Info

Publication number
AR084844A1
AR084844A1 ARP120100114A ARP120100114A AR084844A1 AR 084844 A1 AR084844 A1 AR 084844A1 AR P120100114 A ARP120100114 A AR P120100114A AR P120100114 A ARP120100114 A AR P120100114A AR 084844 A1 AR084844 A1 AR 084844A1
Authority
AR
Argentina
Prior art keywords
citrus
resistance
canker
plant
citrus plant
Prior art date
Application number
ARP120100114A
Other languages
English (en)
Original Assignee
Jones Jeffrey B
Lahaye Thomas
Staskawicz Brian J
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jones Jeffrey B, Lahaye Thomas, Staskawicz Brian J filed Critical Jones Jeffrey B
Publication of AR084844A1 publication Critical patent/AR084844A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263—Ablation; Apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance

Abstract

Se proporcionan métodos y composiciones para preparar plantas cítricas con mejorada resistencia al cancro asiático de los Cítricos (ACC) y otras formas de cancro de los cítricos causadas por Xanthonomas. Los métodos incluyen la transformación de las células de plantas de cítricos con constructos de polinucleótidos que comprenden un promotor operativamente ligado a una secuencia de nucleótidos que codifica una proteína que es capaz de disparar la muerte celular en una planta cítrica. Los promotores son inducibles por una o varias cepas de Xanthomonas que causan cancro de los cítricos. También se proporcionan moléculas aisladas de ácido nucleico y casetes de expresión que comprenden tales constructos de polinucleótidos y promotores. Además se proporcionan plantas cítricas con mejorada resistencia a cancro de los cítricos.Reivindicación 1: Un método para preparar una planta cítrica con mayor resistencia al cancro de los cítricos, que comprende: (a) transformar establemente al menos una célula vegetal de cítrico con un constructo de polinucleótido que comprende un promotor operativamente ligado a una secuencia de nucleótidos que codifica una proteína de ejecución, en donde el promotor comprende al menos una caja UPT y en donde la proteína de ejecución es capaz de disparar la muerte celular en una planta cítrica; y (b) regenerar una planta cítrica transformada de la célula de planta cítrica transformada, en donde la planta cítrica transformada comprende mayor resistencia a al menos una cepa de Xanthomonas que causa el cancro de los cítricos.
ARP120100114A 2011-01-14 2012-01-12 Arboles de citricos con resistencia a cancro de los citricos AR084844A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161433192P 2011-01-14 2011-01-14
US201161433929P 2011-01-18 2011-01-18

Publications (1)

Publication Number Publication Date
AR084844A1 true AR084844A1 (es) 2013-06-26

Family

ID=45529233

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120100114A AR084844A1 (es) 2011-01-14 2012-01-12 Arboles de citricos con resistencia a cancro de los citricos

Country Status (6)

Country Link
US (1) US20140137292A1 (es)
CN (1) CN103354715A (es)
AR (1) AR084844A1 (es)
BR (1) BR112013017955A2 (es)
MX (1) MX2013007852A (es)
WO (1) WO2012097128A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10640784B2 (en) * 2015-07-15 2020-05-05 The Texas A&M University System Pathogen resistant citrus compositions, organisms, systems, and methods
CN109837284B (zh) * 2017-11-28 2022-10-04 华中农业大学 柑橘天然抑菌蛋白CsLTP3制备及其应用
CN109880830B (zh) * 2019-04-04 2022-07-12 中国农业科学院郑州果树研究所 桃多肽激素合成基因PpRGF1及其应用
CN113388620B (zh) * 2021-07-09 2022-08-26 赣南师范大学 一种与柑橘属植物抗病相关的FcRGA1基因、引物对、沉默载体和应用
CN115976064B (zh) * 2023-02-10 2023-09-15 西南大学 调控柑橘溃疡病抗性的CsRBOH5突变基因、表达载体及应用

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Also Published As

Publication number Publication date
MX2013007852A (es) 2013-09-26
BR112013017955A2 (pt) 2019-09-24
WO2012097128A1 (en) 2012-07-19
CN103354715A (zh) 2013-10-16
US20140137292A1 (en) 2014-05-15

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