AR052487A1 - TRANSFORMED CEPA OF ESCHERICHIA COLI PRODUCER OF INTRACELLULAR BIOPOLIMEROS, PROCEDURE TO PRODUCE BIOPOLIMEROS AND PROCEDURE TO PRODUCE AT LEAST A HETEROLOGICAL POLYPEPTIDE IN A E. COLI CULTIVATED IN MICROAEROBIOSIS strain - Google Patents

TRANSFORMED CEPA OF ESCHERICHIA COLI PRODUCER OF INTRACELLULAR BIOPOLIMEROS, PROCEDURE TO PRODUCE BIOPOLIMEROS AND PROCEDURE TO PRODUCE AT LEAST A HETEROLOGICAL POLYPEPTIDE IN A E. COLI CULTIVATED IN MICROAEROBIOSIS strain

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Publication number
AR052487A1
AR052487A1 ARP060100619A ARP060100619A AR052487A1 AR 052487 A1 AR052487 A1 AR 052487A1 AR P060100619 A ARP060100619 A AR P060100619A AR P060100619 A ARP060100619 A AR P060100619A AR 052487 A1 AR052487 A1 AR 052487A1
Authority
AR
Argentina
Prior art keywords
biopolimeros
procedure
produce
transformed
strain
Prior art date
Application number
ARP060100619A
Other languages
English (en)
Inventor
Almeida Alejandra De
Ruben Omar Fernandez
Miguel Angel Galvagno
Veatriz Silvia Mendez
Pablo Ivan Nikel
Maria Julia Pettinari
Jimena Alicia Ruiz
Original Assignee
Consejo Nac Invest Cient Tec
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Nac Invest Cient Tec filed Critical Consejo Nac Invest Cient Tec
Priority to ARP060100619A priority Critical patent/AR052487A1/es
Publication of AR052487A1 publication Critical patent/AR052487A1/es

Links

Abstract

Cepa transformada de Escherichia coli productora de biopolímeros intracelulares, por ejemplo de PHB, que comprende una modificacion en el gen arcA endogeno y tiene la capacidad de ser altamente productora de dichos biopolímeros en condiciones de microaerobiosis. La modificacion del gen ArcA endogeno puede ser una delecion total o parcial, o puede ser una mutacion arcA2, en donde dicha mutacion comprende por ejemplo la arcA2::IS10 o tiene la secuencia SEQ ID No.1. Dicha cepa produce por ejemplo PHB en condiciones de microaerobiosis, por ejemplo a una tension de oxígeno disuelto en el medio de entre 1mM y 45mM, sin requerir el agregado exogeno de aire u oxígeno en el fermentador durante todo el proceso de produccion. Se describe además un procedimiento ara producir eficientemente a biopolímeros tales como PHB en una cepa de E. Coli transformada y un procedimiento para producir polipéptidos altamente reducidos, también en cepas de E. Coli transformadas.Transformed strain of Escherichia coli producing intracellular biopolymers, for example PHB, which comprises a modification in the endogenous arcA gene and has the ability to be highly producing said biopolymers under microaerobiosis conditions. The modification of the endogenous ArcA gene can be a total or partial deletion, or it can be an arcA2 mutation, wherein said mutation comprises for example the arcA2 :: IS10 or has the sequence SEQ ID No.1. Said strain produces, for example, PHB under conditions of microaerobiosis, for example at a tension of dissolved oxygen in the medium between 1mM and 45mM, without requiring the exogenous addition of air or oxygen in the fermenter during the entire production process. A method for efficiently producing biopolymers such as PHB in a transformed E. coli strain and a process for producing highly reduced polypeptides, also in transformed E. coli strains, is also described.
ARP060100619A 2006-02-21 2006-02-21 TRANSFORMED CEPA OF ESCHERICHIA COLI PRODUCER OF INTRACELLULAR BIOPOLIMEROS, PROCEDURE TO PRODUCE BIOPOLIMEROS AND PROCEDURE TO PRODUCE AT LEAST A HETEROLOGICAL POLYPEPTIDE IN A E. COLI CULTIVATED IN MICROAEROBIOSIS strain AR052487A1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ARP060100619A AR052487A1 (es) 2006-02-21 2006-02-21 TRANSFORMED CEPA OF ESCHERICHIA COLI PRODUCER OF INTRACELLULAR BIOPOLIMEROS, PROCEDURE TO PRODUCE BIOPOLIMEROS AND PROCEDURE TO PRODUCE AT LEAST A HETEROLOGICAL POLYPEPTIDE IN A E. COLI CULTIVATED IN MICROAEROBIOSIS strain

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP060100619A AR052487A1 (es) 2006-02-21 2006-02-21 TRANSFORMED CEPA OF ESCHERICHIA COLI PRODUCER OF INTRACELLULAR BIOPOLIMEROS, PROCEDURE TO PRODUCE BIOPOLIMEROS AND PROCEDURE TO PRODUCE AT LEAST A HETEROLOGICAL POLYPEPTIDE IN A E. COLI CULTIVATED IN MICROAEROBIOSIS strain

Publications (1)

Publication Number Publication Date
AR052487A1 true AR052487A1 (es) 2007-03-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ARP060100619A AR052487A1 (es) 2006-02-21 2006-02-21 TRANSFORMED CEPA OF ESCHERICHIA COLI PRODUCER OF INTRACELLULAR BIOPOLIMEROS, PROCEDURE TO PRODUCE BIOPOLIMEROS AND PROCEDURE TO PRODUCE AT LEAST A HETEROLOGICAL POLYPEPTIDE IN A E. COLI CULTIVATED IN MICROAEROBIOSIS strain

Country Status (1)

Country Link
AR (1) AR052487A1 (es)

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