AR034911A1 - Metodo de transformacion para obtener plantas libres de marcadores y plantas obtenidas con el mismo - Google Patents

Metodo de transformacion para obtener plantas libres de marcadores y plantas obtenidas con el mismo

Info

Publication number
AR034911A1
AR034911A1 ARP020102833A AR034911A1 AR 034911 A1 AR034911 A1 AR 034911A1 AR P020102833 A ARP020102833 A AR P020102833A AR 034911 A1 AR034911 A1 AR 034911A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
plants
markers
acid according
allows
Prior art date
Application number
Other languages
English (en)
Original Assignee
Avebe Coop Verkoop Prod
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avebe Coop Verkoop Prod filed Critical Avebe Coop Verkoop Prod
Publication of AR034911A1 publication Critical patent/AR034911A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Reivindicación 1: Un ácido nucleico recombinante que comprende una construcción de T-ADN que permite la transferencia de dicha construcción al genoma de la célula de una planta; dicha construcción está provista de un ácido nucleico extrano que está libre de ácido nucleico que codifica un marcador selectivo. Reivindicación 6: Un ácido nucleico de acuerdo a cualquiera de las reivindicaciones 1 a 5, en donde dicho ácido nucleico extrano permite la regulación de la expresión de un gen objetivo en dicho genoma. Reivindicación 9: Un ácido nucleico de acuerdo con la reivindicación 6, 7 u 8, en donde dicho gen objetivo codifica una enzima sintasa de almidón ligada a gránulo (GBSSI). Reivindicación 10: Un ácido nucleico de acuerdo con cualquiera de las reivindicaciones 1 a 5, en donde dicho ácido nucleico extrano permite la expresión de un polipéptido heterólogo en dicha célula de planta. Reivindicación 12: Un ácido nucleico de acuerdo con la reivindicación 11, en donde dicha enzima comprende una dihidrodipicolinato sintasa (DHPS) con preferencia insensible a la contrarreacción.
ARP020102833 2001-07-27 2002-07-26 Metodo de transformacion para obtener plantas libres de marcadores y plantas obtenidas con el mismo AR034911A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP01202878A EP1279737A1 (en) 2001-07-27 2001-07-27 Transformation method for obtaining marker-free plants

Publications (1)

Publication Number Publication Date
AR034911A1 true AR034911A1 (es) 2004-03-24

Family

ID=8180723

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP020102833 AR034911A1 (es) 2001-07-27 2002-07-26 Metodo de transformacion para obtener plantas libres de marcadores y plantas obtenidas con el mismo

Country Status (9)

Country Link
US (1) US20050097641A1 (es)
EP (3) EP1279737A1 (es)
CN (1) CN1535316B (es)
AR (1) AR034911A1 (es)
AU (1) AU2002318072B2 (es)
BR (1) BR0211508A (es)
CA (1) CA2454793A1 (es)
MX (1) MXPA04000667A (es)
WO (1) WO2003010319A2 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004081184A2 (en) 2003-03-07 2004-09-23 Seminis Vegetable Seeds, Inc. Markerless transformation
CA2661181C (en) * 2006-08-31 2015-07-21 Monsanto Technology Llc Identifying transgenic corn plants without screening or selection
KR100756889B1 (ko) 2006-09-29 2007-09-07 한국생명공학연구원 아그로박테리움 라이조제네스를 이용한 선별 마커가 결손된형질전환 감자 모상근
EP1949785A1 (en) 2007-01-26 2008-07-30 Coöperatie AVEBE U.A. Use of R-genes as a selection marker in plant transformation and use of cisgenes in plant transformation
JPWO2008105509A1 (ja) * 2007-02-28 2010-06-03 日本たばこ産業株式会社 選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法
JP2011120478A (ja) * 2008-03-31 2011-06-23 Japan Tobacco Inc アグロバクテリウム菌による形質転換植物の作成方法
EP2119786A1 (en) 2008-05-13 2009-11-18 Expressive Research B.V. Increased production of health-promoting compounds in plants
UA110021C2 (uk) 2009-09-18 2015-11-10 Спосіб забезпечення принаймні часткової резистентності чи підвищення резистентності у рослини проти ооміцетної інфекції
BR112012014105B1 (pt) 2009-12-04 2019-01-29 Temasek Life Sciences Laboratory Limited método para a transformação de plantas do gênero jagtrofa
NL2004624C2 (en) 2010-04-28 2011-11-01 Stichting Dienst Landbouwkundi A new glycosyltransferase protein and its role in the metabolism of phenylpropanoid volatiles in tomato.
EP2390332A1 (en) 2010-05-31 2011-11-30 Coöperatie Avebe U.A. Cloning and exploitation of a functional R-gene from Solanum x edinense
CA2873360A1 (en) 2012-05-25 2013-11-28 Wageningen Universiteit New plant resistance gene
WO2014112875A1 (en) 2013-01-17 2014-07-24 Wageningen Universiteit A new method to provide resistance to bacterial soft rot in plants
NL2011980C2 (en) 2013-12-17 2015-06-18 Univ Leiden New effects of plant ahl proteins.
NL2014107B1 (en) 2015-01-09 2016-09-29 Limgroup B V New methods and products for breeding of asparagus.
WO2020226499A1 (en) 2019-05-07 2020-11-12 Agventure B.V. Self-compatibility in cultivated potato
WO2021015616A1 (en) 2019-07-22 2021-01-28 Wageningen Universiteit Lrr-rlkii receptor kinase interaction domains
CN113293170B (zh) * 2021-05-27 2022-10-18 中国热带农业科学院热带生物技术研究所 调控木薯淀粉含量的基因MeTIR1及其应用

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3847888A (en) * 1972-12-01 1974-11-12 Allied Chem Ultra-high molecular weight polyethylene molding powder and molding process
GB1470850A (en) * 1972-12-01 1977-04-21 Allied Chem Moulding poder
EP0131623B2 (en) 1983-01-17 1999-07-28 Monsanto Company Chimeric genes suitable for expression in plant cells
CA1340766C (en) 1984-12-24 1999-09-28 Clive Waldron Selectable marker for development of vectors and transformation systems in plants
CA1313830C (en) 1985-08-07 1993-02-23 Dilip Maganlal Shah Glyphosate-resistant plants
CN87100603A (zh) 1987-01-21 1988-08-10 昂科公司 抗黑素瘤疫苗
US6037527A (en) 1987-11-19 2000-03-14 Monsanto Company Expression of proteins in plants using an AMV coat protein leader sequence
DE68918494T2 (de) 1988-05-17 1995-03-23 Lubrizol Genetics Inc Pflanzliches Ubiquitinpromotorsystem.
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5178139A (en) * 1990-03-05 1993-01-12 Stephen P. Angelillo Absorbent pad and thermal pack
JPH04222527A (ja) 1990-12-19 1992-08-12 Japan Tobacco Inc トマトの形質転換方法
US5484620A (en) * 1990-12-22 1996-01-16 Schenk-Filterbau Gesellschaft Mit Beschrankter Haftung Method of manufacturing stabilizing and/or filtering aids for use in the processing of liquids, especially beverages
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
JPH04330234A (ja) 1991-03-20 1992-11-18 Japan Tobacco Inc キュウリモザイクウィルス抵抗性トマト及びその作出方法
AU662202B2 (en) * 1992-02-24 1995-08-24 Montell North America Inc. Polyolefin compositions having good transparency and impact resistance
DE4211529A1 (de) * 1992-04-06 1993-10-07 Herding Entstaubung Filterelement mit einem formstabilen, durchlässig-porösen Kunststoff-Formkörper
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
BR9306802A (pt) 1992-07-27 1998-12-08 Pioneer Hi Bred Int Processo independente de genótipos para produção de planta de soja transgénica e processo de regeneração de plantas de soja a partir de nodos cotiledonais
DE69331908T2 (de) 1992-10-05 2002-12-12 North Carolina State University, Raleigh Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen
EP0699239A1 (en) 1993-04-19 1996-03-06 Novartis AG Genetic stabilizing elements
AU1121395A (en) * 1993-12-08 1995-06-27 Japan Tobacco Inc. Method of transforming plant and vector therefor
JP3256952B2 (ja) 1994-11-09 2002-02-18 日本製紙株式会社 植物への遺伝子導入用ベクター、並びにこれを用いた遺伝子導入植物の作成方法及び植物への遣伝子多重導入方法
MX9707666A (es) 1995-04-06 1997-11-29 Seminis Vegetables Proceso de seleccion de celulas de planta transgenica.
AR006928A1 (es) * 1996-05-01 1999-09-29 Pioneer Hi Bred Int Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.
WO1998051806A2 (en) * 1997-05-16 1998-11-19 Pioneer Hi-Bred International, Inc. Recovery of transformed plants without selectable markers by nodal culture and enrichment of transgenic sectors
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
EP1009842B1 (en) 1997-06-30 2004-05-19 Syngenta Mogen B.V. Plasmids for plant transformation and method for using the same
US6153811A (en) 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
PT1068311E (pt) 1998-04-08 2011-07-20 Commw Scient Ind Res Org Processos e meios de obter fenótipos modificados
EP0959133A1 (en) 1998-05-22 1999-11-24 Centrum Voor Plantenveredelings- En Reproduktieonderzoek (Cpro-Dlo) A process for inhibiting expression of genes
EP1117816B1 (en) * 1998-10-01 2005-12-21 Pioneer Hi-Bred International, Inc. Method of plant transformation
DE69921180T2 (de) 1998-12-01 2005-02-17 Dow Agrosciences Llc, Indianapolis Künstliche matrix-anheftungsregion zur erhöhung der expression von in pflanzen eingeführten genen
ATE331035T1 (de) 1998-12-11 2006-07-15 Monsanto Technology Llc Verfahren zur verbesserung der effizienz agrobakterium-vermittelter transformation von pflanzen
DE69924005T2 (de) 1998-12-22 2006-04-13 National Research Council Canada, Ottawa Transgene pflanzen mit konditional lethalem gen
EP1111063A1 (en) * 1999-12-24 2001-06-27 Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.V. Modified DHPS genes

Also Published As

Publication number Publication date
EP1279737A1 (en) 2003-01-29
WO2003010319A2 (en) 2003-02-06
US20050097641A1 (en) 2005-05-05
WO2003010319A3 (en) 2003-10-30
EP1412507A2 (en) 2004-04-28
CN1535316B (zh) 2014-09-17
CA2454793A1 (en) 2003-02-06
CN1535316A (zh) 2004-10-06
MXPA04000667A (es) 2004-04-05
AU2002318072B2 (en) 2007-09-06
BR0211508A (pt) 2004-09-14
EP2366788A1 (en) 2011-09-21

Similar Documents

Publication Publication Date Title
AR034911A1 (es) Metodo de transformacion para obtener plantas libres de marcadores y plantas obtenidas con el mismo
HUP9902123A2 (hu) Eljárások stabilan transzformált termékeny búza előállítására Agrobacterium-közvetítette transzformáció alkalmazásával
ATE507300T1 (de) Rekombinante konstrukte und ihre verwendung bei der reduzierung der genexpression
McKnight et al. Telomeres, telomerase, and stability of the plant genome
BR0306978A (pt) ácido nucleico isolado, vetor de expressão, célula de planta, planta, método para produzir uma planta transgênica, composição, métodos para controlar o crescimento de plantas, para estimular a tolerância a estresse de uma planta e para inibir o crescimento de uma planta transgênica, e, uso de um ácido nucleico
AU2002246962A1 (en) Maize chloroplast aldolase promoter compositions and methods for use thereof
BR0009471A (pt) Composições e processos para modificação de transcrição de gene
AR035799A1 (es) Toxinas insecticidas aisladas de bacillus thuringiensis y sus usos.
BR9007523A (pt) Construcoes de dna,celulas de plantas,plantas,sementes,bulbos ou mudas
BR0211726A (pt) Plantas de algodão tolerantes a herbicida e métodos para produção e identificação das mesmas
MXPA06000092A (es) Transformacion de cloroplasto de la lenteja de agua.
BRPI0515431A (pt) ácido nucleico de proteìna de metabolismo de lipìdeos isolado, métodos de produzir uma planta transgênica e de modular o nìvel de um composto de armazenamento de semente em uma planta, e, planta transgênica
AR050809A1 (es) Modificacion del desarrollo y la morfologia de las plantas
BR0112643A (pt) Tioesterases de acil coenzima a
BRPI0418048A (pt) ácido nucleico de lmp isolado, vetor de expressão, e, métodos para produzir uma planta transgênica e para modular o nìvel de um composto de armazenagem de semente em uma planta
SI1309698T1 (sl) Citokrom p450 encim zdruĺ˝en s sintezo delta-12-epoksi skupin v maĺ äśobnih kislinah rastlin
Navarre et al. Identification, gene cloning and expression of serine proteases in the extracellular medium of Nicotiana tabacum cells
BRPI0506620A (pt) polinucleotìdeo isolado, subfragmento funcionalmente equivalente do polinucleotìdeo isolado, fragmento de ácido nucléico isolado, construção de dna recombinante, planta, semente, célula vegetal ou tecido vegetal transformado, método de alteração da estrutura de raiz, método de isolamento de fragmentos de ácido nucléico, método de mapeamento de variações genéticas e método de cultivo molecular
DK1301610T3 (da) Fremgangsmåde til påvirkning af sinapinindholdet i transgene planteceller og planter
DE60239462D1 (de) Auf replizierende vektoren basierende genexpression in plastiden
EP1190039A4 (en) FOR SINCODING GENES AND THEIR USE
BR0210246A (pt) Construções recombinantes úteis para alterar o tamanho do embrião/endosperma de sementes
AR015757A1 (es) Fragmento aislado de acidos nucleicos que codifica toda o una porcion sustancial de proteinas reguladoras del ciclo celular en plantas y semillas, genquimerico, celula huesped transformada, polipeptido, metodo para alterar el nivel de expresion de dicha proteina, metodo para obtener dicho fragmento
ES2100173T3 (es) Procedimiento para la integracion de adn dirigida al sitio en el genoma de plantas.
DE60109790D1 (de) Konstitutiver promoter aus arabidopsis

Legal Events

Date Code Title Description
FG Grant, registration
FD Application declared void or lapsed, e.g., due to non-payment of fee