WO2009092386A3 - A new method to measure and characterize microvesicles in the human body fluids - Google Patents

A new method to measure and characterize microvesicles in the human body fluids Download PDF

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Publication number
WO2009092386A3
WO2009092386A3 PCT/EE2009/000002 EE2009000002W WO2009092386A3 WO 2009092386 A3 WO2009092386 A3 WO 2009092386A3 EE 2009000002 W EE2009000002 W EE 2009000002W WO 2009092386 A3 WO2009092386 A3 WO 2009092386A3
Authority
WO
WIPO (PCT)
Prior art keywords
characterize
human body
body fluids
exosomes
microvesicles
Prior art date
Application number
PCT/EE2009/000002
Other languages
French (fr)
Other versions
WO2009092386A2 (en
WO2009092386A8 (en
Inventor
Stefano Fais
Mariantonia Logozzi
Original Assignee
Hansabiomed Oü
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP09704302.0A priority Critical patent/EP2245458B1/en
Application filed by Hansabiomed Oü filed Critical Hansabiomed Oü
Priority to BRPI0906683A priority patent/BRPI0906683B8/en
Priority to CA2713196A priority patent/CA2713196C/en
Priority to ES09704302.0T priority patent/ES2613670T3/en
Priority to MX2010008164A priority patent/MX2010008164A/en
Priority to RU2010135526/15A priority patent/RU2520741C2/en
Priority to AU2009207927A priority patent/AU2009207927B2/en
Priority to CN200980111452.4A priority patent/CN102317778B/en
Priority to JP2010543379A priority patent/JP5583600B2/en
Publication of WO2009092386A2 publication Critical patent/WO2009092386A2/en
Publication of WO2009092386A3 publication Critical patent/WO2009092386A3/en
Publication of WO2009092386A8 publication Critical patent/WO2009092386A8/en
Priority to US13/290,207 priority patent/US20120058492A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G01N33/567—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds utilising isolate of tissue or organ as binding agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/5743—Specifically defined cancers of skin, e.g. melanoma
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2814—Dementia; Cognitive disorders
    • G01N2800/2828—Prion diseases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Oncology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • AIDS & HIV (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This disclosure provides a method to capture, detect, characterize and quantify human exosomes in small volumes of human body fluids by using a sandwich ELISA test. This method allows a full characterization of an exosome preparation, thus providing a tool to distinguish a disease-related condition from a healthy state, by the use of a non-invasive assay. In fact, this method may be useful in either screening, diagnosis and prognosis of tumors, with a simple plasma sample. At the same time measurement of circulating exosomes may provide information on the level of tumor mass present in a patient. The method provided here is suitable to evaluate presence of some infectious and/or transmissible agents, such as viral proteins or prion proteins, within circulating exosomes.
PCT/EE2009/000002 2008-01-25 2009-01-26 A new method to measure and characterize microvesicles in the human body fluids WO2009092386A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
RU2010135526/15A RU2520741C2 (en) 2008-01-25 2009-01-26 Novel method for measuring and describing microvesicles in human fluids
BRPI0906683A BRPI0906683B8 (en) 2008-01-25 2009-01-26 method for measuring and characterizing microvesicles in human body fluids
CA2713196A CA2713196C (en) 2008-01-25 2009-01-26 A method to measure and characterize microvesicles in the human body fluids
ES09704302.0T ES2613670T3 (en) 2008-01-25 2009-01-26 A new method to measure and characterize microvesicles in human body fluids
MX2010008164A MX2010008164A (en) 2008-01-25 2009-01-26 A new method to measure and characterize microvesicles in the human body fluids.
EP09704302.0A EP2245458B1 (en) 2008-01-25 2009-01-26 A new method to measure and characterize microvesicles in the human body fluids
AU2009207927A AU2009207927B2 (en) 2008-01-25 2009-01-26 A new method to measure and characterize microvesicles in the human body fluids
CN200980111452.4A CN102317778B (en) 2008-01-25 2009-01-26 A new method to measure and characterize microvesicles in the human body fluids
JP2010543379A JP5583600B2 (en) 2008-01-25 2009-01-26 A novel method for measuring and characterizing microvesicles in human body fluids
US13/290,207 US20120058492A1 (en) 2008-01-25 2011-11-07 Method and a Kit To Detect Malignant Tumors and Provide a Prognosis

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
USNONE 2005-12-29
US6252808P 2008-01-25 2008-01-25
US61/062,528 2008-01-25
US12/321,412 US8617806B2 (en) 2008-01-25 2009-01-21 Method to measure and characterize microvesicles in the human body fluids
US12/321,412 2009-01-21

Publications (3)

Publication Number Publication Date
WO2009092386A2 WO2009092386A2 (en) 2009-07-30
WO2009092386A3 true WO2009092386A3 (en) 2009-09-17
WO2009092386A8 WO2009092386A8 (en) 2010-10-14

Family

ID=41064551

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EE2009/000002 WO2009092386A2 (en) 2008-01-25 2009-01-26 A new method to measure and characterize microvesicles in the human body fluids

Country Status (12)

Country Link
US (1) US8617806B2 (en)
EP (1) EP2245458B1 (en)
JP (1) JP5583600B2 (en)
CN (1) CN102317778B (en)
AU (1) AU2009207927B2 (en)
BR (1) BRPI0906683B8 (en)
CA (1) CA2713196C (en)
ES (1) ES2613670T3 (en)
MX (1) MX2010008164A (en)
PL (1) PL2245458T3 (en)
RU (1) RU2520741C2 (en)
WO (1) WO2009092386A2 (en)

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Also Published As

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WO2009092386A2 (en) 2009-07-30
BRPI0906683B8 (en) 2021-07-27
JP2011510309A (en) 2011-03-31
US20090220944A1 (en) 2009-09-03
MX2010008164A (en) 2010-11-25
ES2613670T3 (en) 2017-05-25
JP5583600B2 (en) 2014-09-03
CN102317778A (en) 2012-01-11
EP2245458A2 (en) 2010-11-03
AU2009207927A1 (en) 2009-07-30
BRPI0906683A2 (en) 2017-08-22
WO2009092386A8 (en) 2010-10-14
BRPI0906683B1 (en) 2021-03-02
RU2010135526A (en) 2012-02-27
CN102317778B (en) 2015-05-13
AU2009207927B2 (en) 2015-04-09
CA2713196A1 (en) 2009-07-30
CA2713196C (en) 2014-06-17
PL2245458T3 (en) 2017-08-31
US8617806B2 (en) 2013-12-31
RU2520741C2 (en) 2014-06-27
EP2245458B1 (en) 2016-10-26

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