WO2003085107A1 - Cellules à génome modifié - Google Patents
Cellules à génome modifié Download PDFInfo
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- WO2003085107A1 WO2003085107A1 PCT/JP2003/004507 JP0304507W WO03085107A1 WO 2003085107 A1 WO2003085107 A1 WO 2003085107A1 JP 0304507 W JP0304507 W JP 0304507W WO 03085107 A1 WO03085107 A1 WO 03085107A1
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- Prior art keywords
- antibody
- cells
- antibody composition
- sugar chain
- fucose
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
- C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Definitions
- the present invention relates to an N-glycoside-linked complex type sugar chain, wherein the activity of an enzyme involved in sugar chain modification in which the 6-position of fucose is ⁇ -linked to the 6-position of N-acetyldarcosamine at the reducing end is lower or higher than that of the parent cell line.
- the present invention relates to a cell whose genome has been modified so as to be deleted and a method for producing an antibody molecule using the cell. Background art
- N-glycoside-type sugar chain binding sites There are two N-glycoside-type sugar chain binding sites in the Fc region of an antibody IgG molecule, and IgG in serum usually contains sialic acid or bisecting N-acetylethyl glucoside at these sites.
- Complex-type sugar chains with multiple branches with little addition of samin are bound. It is known that there is diversity in the addition of galactose at the non-reducing end of this complex type sugar chain and the addition of fucose to N-acetyldarcosamine at the reducing end [Biochemistry] , 36, 130, 1997] o
- sugar chain gene that is, a gene for a glycosyltransferase that synthesizes a sugar chain and a gene for a glycolytic enzyme that degrades a sugar chain.
- the following describes the biosynthesis of N-daricoside-linked sugar chains.
- Glycoproteins undergo sugar chain modification in the lumen of the endoplasmic reticulum (hereinafter referred to as ER).
- ER endoplasmic reticulum
- N-acetyldarcosamine transferase I GnTI
- GnTII N-acetyldarcosamine transferase II
- 1,6-fucosyltransferase 1,6-fucosyltransferase
- the addition of fucose to the N-glycidyl-linked glycan at the reducing end of the N-glycoside-linked sugar chain of the antibody results in the antibody-dependent cytotoxicity of the antibody.
- ADCC activity (Hereinafter referred to as ADCC activity) has been reported to change significantly
- the present invention relates to the following (1) to (43).
- 1,6-fucosinoretransferase is an enzyme involved in sugar chain modification in which fucose is connected to position 6 of fucose at position 6 of ⁇ ⁇ -glycoside-linked complex type sugar chain reducing terminal
- the cell according to any one of (1) to (3).
- al, 6-fucosyltransferase is a protein encoded by a DNA selected from the group consisting of the following (a), (b), (c) and (d).
- the 1,6-fucosyltransferase is a protein selected from the group consisting of the following (a), (b), (c) (d), (e) and (f): ).
- mouse myeloid cell line SP2 / 0-Agl4 cells (d) mouse myeloid cell line SP2 / 0-Agl4 cells; (e) BHK cells derived from Syrian hamster-kidney tissue;
- the antibody-dependent cytotoxicity is high, and the antibody composition is one of all N-glycoside-linked complex type sugar chains that bind to the Fc region contained in the antibody composition.
- the 1-position of the fucose is located at the 6-position of N-acetyldarcosamine at the reducing end of the N-daricoside-linked complex-type sugar chain (3 ⁇ 4 The cell according to (14).
- the antibody composition having a high antibody-dependent cytotoxicity activity is the N-acetyldarco at the reducing end of the sugar chain among all N-glycoside-linked complex type sugar chains that bind to the Fc region contained in the antibody composition.
- the antibody composition having a high antibody-dependent cytotoxicity activity is an antibody composition having no sugar chain by combining N-acetylglucosamine at the reducing end of sugar chain and fucose, (13) to ( 16.
- a method for producing an antibody composition comprising using the cell according to any one of (10) to (: 17).
- a method for producing an antibody composition comprising:
- the antibody composition having a high antibody-dependent cytotoxicity is characterized in that, among all N-glycoside-linked complex-type sugar chains that bind to the Fc region contained in the antibody composition, N-acetyldarco at the reducing end of the sugar chain is used. (20) The method according to (20), wherein the ratio of sugar chains in which samin and fucose are not bound is higher than that of the antibody composition produced by the parent cell line.
- the sugar chain to which fucose is not bound is a sugar chain in which the 1-position of the fucose is not a-linked to the 6-position of N-acetyldarcosamine at the reducing end of the N-daricoside-linked complex type sugar chain. And (21).
- An antibody composition having a high antibody-dependent cytotoxic activity is one of the complex N-dalicoside-linked sugar chains that bind to the Fc region contained in the antibody composition.
- the antibody composition having a high antibody-dependent cytotoxic activity is an antibody composition having no sugar chain in which N-acetyldarcosamine at the sugar chain reducing end and fucose are bound,
- the transgenic non-human animal is an animal selected from the group consisting of a red sea lion, a sheep, a goat, a pig, a poma, a mouse, a rat, a chicken, a monkey, and a heron.
- the transgenic non-human animal according to any one of (27 :) to (29) is a plant or a body or a body fluid containing an antibody composition introduced from the bred animal or plant. And obtaining the antibody composition from the tissue or body fluid.
- (31) Obtaining the antibody-producing cells of the transgenic non-human animal or plant or the progeny thereof according to any one of (26) to (29), and culturing the obtained antibody-producing cells in a medium And producing and accumulating the antibody composition in the culture, and collecting the antibody composition from the culture.
- the antibody composition is an antibody composition having higher antibody-dependent cytotoxic activity than an antibody composition obtained from a non-human animal or plant whose genome has not been modified, or a progeny thereof. 30) or the method according to (31).
- the antibody composition having a high antibody-dependent cytotoxicity is characterized in that, among all N-dalicoside-linked complex type sugar chains that bind to the Fc region contained in the antibody composition, N-acetyldarco at the reducing end of the sugar chain is used.
- the ratio of a sugar chain in which samin and fucose are not bound is higher than that of an antibody composition obtained from a non-human animal or plant whose genome has not been modified, or a progeny thereof. the method of.
- the sugar chain to which fucose is not bound is a sugar chain in which the 1-position of the fucose is not ⁇ -linked to the 6-position of N-acetyldarcosamine at the reducing end of the N-daricoside-linked complex type sugar chain.
- An antibody composition having a high antibody-dependent cytotoxic activity is characterized in that, among all N-daricoside-linked complex-type sugar chains that bind to the Fc region contained in the antibody composition, N-acetyldarco at the reducing end of the sugar chain is used.
- the antibody composition having high antibody-dependent cytotoxic activity is an antibody composition having no sugar chain in which N-acetyldarcosamine at the sugar chain reducing end and fucose are bound, (32) to (35). Le, deviation or the method described in paragraph 1.
- An antibody composition comprising an antibody molecule having an N-glycoside-linked sugar chain in the Fc region, wherein all the daricoside-linked complex-type bran chains which bind to the Fc region contained in the antibody composition are selected from the group consisting of: An antibody composition having a sugar chain, wherein fucose is not bound to N-acetyldaricosamine at the chain reducing end.
- the sugar chain to which fucose is not bound is a sugar chain in which the 1-position of the fucose is not a-linked to the 6-position of N-acetyldarcosamine at the reducing end of the N-glycoside-linked complexed bran chain.
- a medicament comprising the antibody composition according to any one of (37) to (40) as an active ingredient.
- the drug is a diagnostic, prophylactic or therapeutic agent for a disease associated with a tumor, a disease associated with allergy, a disease associated with inflammation, an autoimmune disease, a cardiovascular disease, a disease associated with a viral infection or a disease associated with a bacterial infection.
- cells of the present invention the position of fucose at the 6th position of ⁇ ⁇ -acetylglucosamine at the reducing end of ⁇ -daricoside-linked complex type sugar chain,
- hi 1,6-fucose modifying enzyme an enzyme involved in sugar chain modification that binds to ⁇
- the parent cell is a cell before the method for reducing or deleting the activity of 1,6-fucose modifying enzyme is applied to the genome.
- the parent cell is not particularly limited. For example, the following cells can be mentioned.
- NS0 cells The parent cells of NS0 cells are described in the literature such as Bio / Technology (BI0 / TECHN0L0GY), 10, 169 (1992), Biotechnology. Bioengineering (Biotechnol. Bioeng.), 73, 261, (2001), etc. NS0 cells that have been used. Ma There are also NSO cell lines (RCB0213) registered with the RIKEN Cell Development Bank, or sublines obtained by adapting these strains to a growth medium.
- the parental cells of SP2 / 0-Agl4 cells include, for example, Journal 'Op' Immunol. (J. Immunol.), 126, 317, (1981), Nature, 276, 269, (1978), Huyman ' SP2 / 0-Agl4 cells described in the literature such as Antiibodies 'and' Nodipuri Domas (Human Antibodies and Hybridomas), 3, 129, (1992). Also, there are SP2 / 0-Agl4 cells (ATCC CRL-1581) registered with the ATCC, or a substrain (ATCC CRL-1581.1) obtained by adapting these strains to a growth medium.
- CH0 cells derived from Chinese hamster ovary tissue include Journal of Experimental Medicine, 108, 945 (1958), Proc. Natl. Acad. Sci. USA, 60, 1275 (1968), Genetics, 55, 513 (1968). Chromosoma, 41, 129 (1973), Methods in Cell Science, 18, 115 (1996), Radiation Research, 148, 260 (1997), Proc. Natl. Acad. Sci. USA, 77, 4216 (1980), Proc. Natl. Acad. Sci. 60, 1275 (1968), Cell, 6, 121 (1975), and Molecular Cell Genetics, Appendix I, II (p883-900).
- CH0-K1 strains ATCC CCL-61
- DUXB11 strains ATCC CRL-9096
- Pro- 5 strains ATCC CRL-1781 registered with the ATCC
- commercially available CHO-S strains Lifetechnologies Cat # 11619
- the parent cell line of the rat myeloma cell line YB2 / 3HL. P2. Gil. 16Ag. 20 cell includes a cell line established from Y3 / Agl. 2.3 cell (ATCC CRL-1631). Specific examples thereof include YB2 / 3HL. P2. Gil. 16Ag. 20 cells described in references such as J. Cell. Biol. 93, 576 (1982) and Methods Enzymol. 73B, 1 (1981). Is raised. Also, there are YB2 / 3HL. P2. Gil. 16Ag. 20 cells (ATCC CRL-1662) registered with the ATCC, or substrains obtained by adapting these strains to a medium capable of growing.
- a 1,6-Fucose-modifying enzyme is an enzyme that participates in the reaction in which the 6-position of N-acetyltilcosamine at the reducing end of the N-glycoside-linked complex type sugar chain and the 1-position of fucose are ⁇ -linked. Such enzymes are also included.
- the enzyme involved in the reaction in which the 6-position of ⁇ ⁇ -acetyl-darcosamine at the reducing end of ⁇ -glycoside-linked complex-type sugar chain and the 1-position of fucose are ⁇ -linked also includes an enzyme that affects the reaction. W
- CK I, 6-fucose modifying enzyme examples include 1,6-fucosyltransferase ⁇ ⁇ -L-fucosidase.
- the enzyme that influences the ⁇ - bonding reaction between the 6-position of ⁇ ⁇ -acetyldarcosamine at the reducing end of ⁇ -glycoside-linked complex type sugar chain and the 1-position of fucose is the aforementioned 1,6-fucose modifying enzyme Enzymes that affect the activity of the enzyme or that affect the structure of a substance serving as a substrate for the enzyme are also included.
- the al, 6-fucosyltransferase includes a protein encoded by the DNA of the following (a;), (b), (c) or (d), or the following (e), (f) or (g): , (H), (i) or (j).
- DNA that hybridizes under stringent conditions refers to, for example, colony hybridization using a DNA such as a DNA having a base sequence represented by SEQ ID NO: 1 or 2 or a fragment thereof as a probe.
- Chillon method This refers to DNA obtained by using the Lark hybridisation method or Southern blot'hybridization method. Specifically, using a filter on which DNA derived from colonies or plaques has been immobilized, a DNA filter is used.
- the hybridizable DNA is a DNA having at least 60% or more homology with the nucleotide sequence represented by SEQ ID NO: 1 or 2, preferably 70% or more, more preferably 80% or more, and still more preferably 90% or more. % Or more, particularly preferably 95% or more, most preferably 98% or more.
- one or more amino acids in the amino acid sequence represented by SEQ ID NO: 4 or 5 comprise deletion, substitution, insertion and Z or an added amino acid sequence, and ⁇ 1,6-fucosyltransferase activity
- a protein having the following structure is referred to as "Molecula monocroc Jung 2nd edition", “Kyrent Protocornoles 'in' Molecular Biology, Nucleic Acids Research, 10, 6487 (1982), Proc. Natl. Acad. Sci., USA, 79, 6409 (1982), Gene, 34, 315 (1985), Nucleic Acids Research, 13, 4431 (1985), Proc. Natl. Acad. Sci USA, 82, 488 (1985), etc.
- the number of amino acids to be deleted, substituted, inserted and Z or added is one or more, and the number thereof is not particularly limited. Deletion, substitution, The number is such that it can be inserted or added, for example, 1 to several tens, preferably 1 to 20, more preferably 1 to 10, and still more preferably 1 to 5.
- a protein having 80% or more homology with the amino acid sequence represented by SEQ ID NO: 4 or 5 and having a 1,6-fucosyltransferase activity is defined as BLAST (J.
- the protein is at least 80% or more, preferably 85% or more, more preferably 90% or more, further preferably 95% or more, particularly preferably 97% or more, and most preferably 99% or more.
- the activity of an enzyme involved in sugar chain modification in which the 6-position of fucose is attached to the 6-position of N-acetyldarcosamine at the N-glycoside-linked complex type sugar chain reducing terminal is reduced or deleted from the parent cell line.
- genome modification means that a mutation is introduced into the expression control region of the gene of the enzyme so as to decrease or delete the expression of the enzyme, or the genome is altered so as to reduce or delete the function of the enzyme.
- This refers to introducing a mutation into the amino acid sequence of a gene.
- Introducing a mutation refers to modifying a nucleotide sequence such as deletion, substitution, insertion and / or addition to a nucleotide sequence on the genome.
- a cell in which a genomic gene is knocked out refers to a cell that completely suppresses the expression or function of the genomic gene.
- a specific example of a cell in which a genomic gene has been knocked out is a cell in which all or part of a target gene has been deleted from the genome.
- any method can be used as long as the desired genome can be modified, but a genetic method is preferable.
- a specific method for obtaining such cells, any method can be used as long as the desired genome can be modified, but a genetic method is preferable. As a specific method,
- Lectin-resistant cells are cells whose growth is not inhibited even when an effective concentration of lectin is given.
- the effective concentration is the concentration above which the parent cell cannot grow normally. Yes, preferably at the same concentration as the concentration at which the parent cell cannot grow normally, more preferably
- the effective concentration of lectin that it does not inhibit growth may be appropriately determined according to each cell line, usually 10 ⁇ ⁇ / ⁇ 1 ⁇ 10. 0m g / ml, preferably 0. 5 to 2. Omg / Fill with ml.
- a lectin that recognizes a sugar chain structure in which the 6-position of N-acetylglucosamine at the reducing end of the N-glycoside-linked complex type sugar chain and the 1-position of fucose are bonded a lectin that can recognize the sugar chain structure If present, any lectin is included. Specific examples include Lentil lectin LCA (Lentil Agglutinin from Lens Culinaris), Endum bean lectin PSA (Pea Lectin from Pi sum sativum), Broad bean lectin VFA (Agglutinin from Vicia faba), and Hiirochiwan Takelectin AAL (Lectin from Aleuria aurantia) and the like.
- Lentil lectin LCA Lientil Agglutinin from Lens Culinaris
- Endum bean lectin PSA Pea Lectin from Pi sum sativum
- Broad bean lectin VFA Adgglutinin from Vicia faba
- the cells of the present invention may be any cells as long as they can express the antibody molecule, and include yeast, animal cells, insect cells, plant cells, and the like. Specific examples of these cells are described below. Items listed in 3. Specific examples of animal cells include CH0 cells derived from Chinese hamster ovarian fibrous tissue, rat myeloma cell line YB2 / 3HL.P2Gil.16Ag.20 cell, mouse myeloma cell line NSO cell, mouse myeloma Cell line SP 2 / 0—Agl4 cells, Syrian hamster BHK cells derived from kidney tissue, antibody-producing hybridoma cells, human leukemia cell line Namalva cells, embryonic stem cells, fertilized egg cells, and the like.
- the above-mentioned myeloma cells, hybridoma cells, host cells for producing humanized antibodies and human antibodies, and transcells other than humans that produce human antibodies are used in the production of the antibody composition.
- Examples include embryonic stem cells or fertilized egg cells used for producing a digenic animal, and plant cells used for producing a transgenic plant producing a humanized antibody and a human antibody.
- the cells of the present invention can produce an antibody product having higher ADCC activity than the antibody composition produced by the parent cell line.
- the cell of the present invention is characterized in that, among all N-glycoside-linked complex-type sugar chains that bind to the Fc region contained in the antibody composition, N-acetyldarcosamine at the reducing end of the bran chain is bound to fucose. It is possible to produce an antibody composition in which the proportion of unlinked sugar chains is higher than that of the parent cell line.
- the present invention relates to a method for producing an antibody composition, which comprises using the cell of the present invention.
- the antibody composition refers to a composition comprising an antibody molecule having an N-glycoside-linked complex type sugar chain in the Fc region.
- Antibodies are tetramers in which two types of polypeptide chains, a heavy chain and a light chain (hereinafter, referred to as “H chain” and “L chain”, respectively), are associated with each other by two molecules. About one-fourth of the N-terminal side of the H chain and about one-quarter of the N-terminal side of the L chain (each more than 100 amino acids) are called variable regions (hereinafter referred to as V regions). Rich and directly involved in antigen binding. Most of the part other than the V region is called the constant region (hereinafter referred to as C region). Antibody molecules are classified into IgG, IgM, IgA, IgD, and IgE classes based on the homology of the C region.
- the IgG class is further classified into IgG1 to IgG4 subclasses based on the homology of the C region.
- the H chain is divided into four immunoglobulin domains, VH, CHI, CH2, and CH3, from the N-terminal side.There is a highly flexible peptide region called the hinge region between CH1 and CH2, which separates CH1 and CH2.
- Can be The structural unit consisting of CH2 and CH3 after the hinge region is called the Fc region, to which N-glycoside-linked sugar chains are bound. This region is where the Fc receptor and complement bind. (Immunology Illustrated Original 5th edition, published February 10, 2000, Nankodo edition, Introduction to antibody engineering, January 25, 1994 First edition, Citizen Library).
- N-glycoside-linked sugar chains have a basic common core structure as shown in the following structural formula (I) [Biochemical Experiments 23—Glycoprotein Glycan Research Method (Society Press Center), edited by Reiko Takahashi ( 1989)].
- the N-glycoside-linked sugar chain may be any as long as it has the core structure of the above structural formula (I), but it may be a high mannose type in which only mannose is bonded to the non-reducing end of the core structure, It has one or more parallel branches of galactose-N-acetyl-cosamine (hereinafter referred to as Gal-GlcNAc) on the reducing end, and sialic acid and pi-secting N- on the non-reducing end of Gal_GlcNAc.
- Examples include a complex type (complex type) having a structure such as acetilda lucosamine, and a hybrid type having both high mannose type and complex type branches on the non-reducing terminal side of the core structure.
- the Fc region of the antibody molecule has a region in which N-glycoside-linked sugar chains bind one by one, two sugar chains are bound per antibody molecule. Since the N-glucoside-linked sugar chain that binds to the antibody molecule includes any sugar chain containing the core structure represented by the structural formula (I), two N-darcoside-linked sugar chains that bind to the antibody are included. Has many combinations of sugar chains.
- the antibody composition produced using the cell of the present invention may be composed of an antibody molecule having a single sugar chain structure as long as the effects of the present invention can be obtained. Alternatively, it may be composed of antibody molecules having a plurality of different sugar chain structures.
- an antibody composition obtained by the present invention preferably, among all the daricoside-bonded complex-type bran chains that bind to the Fc region contained in the antibody composition, N-acetyldarcosamine at the reducing end of the sugar chain is used.
- An antibody composition in which the ratio of sugar chains to which fucose is not bound is higher than that of an antibody composition produced by a parent cell whose genome has not been modified.
- the product may be composed of an antibody molecule having a single sugar chain structure, or may be composed of a plurality of antibody molecules having different sugar chain structures, as long as the effects of the present invention can be obtained. Is also good.
- an antibody composition preferably, sugars in which fucose is not bound to N-acetylethyldarcosamine at the reducing end of the sugar chain among all glycoside-linked complex-type sugar chains that bind to the Fc region contained in the antibody composition
- An antibody composition whose chain ratio is higher than an antibody composition produced by a non-human animal or plant whose genome has not been modified, or a progeny thereof (hereinafter referred to as a parent individual) is exemplified.
- Transgenic non-human animal or plant or a progeny thereof, whose genome has been modified so that the activity of the 1,6-fucose modifying enzyme is deleted or reduced, is an embryonic stem cell, fertilized egg cell, or plant cell It can be manufactured using.
- the ratio of the sugar chain in which fucose is not bonded to N-acetyltyldarcosamine at the reducing end of the sugar chain among the total N-daricoside-linked complex sugar chains binding to the Fc region contained in the antibody composition is as follows. For all N-glycoside-linked complex-type sugar chains that bind to the Fc region contained in the composition, The ratio of the number.
- a sugar chain in which fucose is not bonded to N-acetyldarcosamine at the reducing end of N-glycoside-linked complex type sugar chain is defined as fucose that is linked to N-acetyldarcosamine at the reducing end of N-glycoside-linked complex type sugar chain.
- a sugar chain that is not ⁇ -linked is exemplified.
- the total N-daricoside-linked complex sugar chains binding to the Fc region contained in the antibody composition of the present invention is preferably 20% or more, more preferably 30% or more, still more preferably 40% or more, particularly preferably 5 °% or more, and most preferably 100%.
- An antibody composition having a higher ADCC activity than the antibody composition produced by the parent cell line or the parent individual includes sugar chain reduction among all N-glycoside-linked complex-type sugar chains that bind to the Fc region contained in the antibody composition.
- the ratio of the sugar chain in which fucose is not bound to the terminal N-acetyltyldarcosamine is higher than the ratio of the antibody composition produced by the parent cell line or the parent individual.
- an antibody composition in which the ratio is at least 2 times, preferably at least 3 times, more preferably at least 5 times, particularly preferably at least 10 times, is mentioned, and binds to an Fc region contained in the antibody composition.
- an antibody composition in which all of the N-glycoside-linked complex-type sugar chains are sugar chains in which the 1-position of fucose is not bonded to the 6-position of N-acetyldarcosamine at the reducing end of the sugar chain.
- an antibody composition in which all of the darcoside-linked complex-type sugar chains are sugar chains in which the 1-position of fucose is not bonded to the 6-position of N-acetyldarcosamine at the reducing end of the sugar chain Means that fucose is substantially undetectable in the sugar chain analysis described in 5 below.
- the antibody composition obtained according to the present invention has higher ADCC activity than the antibody composition consisting of the antibody molecule produced by the parent strain or the parent individual.
- ADCC activity means that in vivo, an antibody that binds to a cell surface antigen such as a tumor cell activates the effector cell through the binding between the antibody Fc region and the Fc receptor present on the surface of the effector cell.
- a cell surface antigen such as a tumor cell
- Examples of the effector cells include killer cells, natural killer cells, and macrophages that have been killed.
- the proportion of sugar chains in which fucose is not bound to N-acetyltyldarcosamine at the sugar chain reducing end, which is contained in a composition comprising an antibody molecule having an N-daricoside-linked complex-type sugar chain in the Fc region, is determined by the ratio of the antibody Using known methods such as hydrazine decomposition and enzymatic digestion from molecules [Biochemical Experimental Method 23-Glycoprotein Glycosylation Research Method (Academic Press Center), Reiko Takahashi (ed., 1989)] It can be determined by labeling the chains with a fluorescent label or an isotope and separating the labeled sugar chains by a single chromatography method. It can also be determined by analyzing the released sugar chains by the HPAED-PAD method [Journal of Liquid 'Chromatography (J. Liq. Chromatogr.), 6, 1577 (1983)]. .
- the antibodies of the present invention include an antibody that recognizes a tumor-associated antigen, an antibody that recognizes an allergy associated with inflammation, an antibody that recognizes an antigen that is associated with circulatory disease, and an antibody that recognizes an autoimmune disease.
- An antibody that recognizes an antigen, or an antibody that recognizes an antigen associated with a virus or bacterial infection is preferable, and IgG is preferable as the antibody class.
- Antibodies that recognize tumor-associated antigens include anti-GD2 antibody (Anticancer Res., 13, 331-336, 1993), anti-GD3 antibody (Cancer Immunol. Immunother., 36, 260-266, 1993), anti-GM2 antibody (Cancer Res., 54, 1511-1516, 1994), anti-HER2 antibody
- Anti-basic fibroblast growth factor antibody Anti-basic fibroblast growth factor antibody, anti-FGF8 receptor antibody (J. Biol. Chem., 265, 16455-16463, 1990) , Anti-basic fibroblast growth factor receptor antibody, anti-insulin-like growth factor antibody (J. Neurosci. Res., 40, 647-659, 1995), anti-insulin-like growth factor receptor antibody (J. Neurosci. Res., 40, 647-659, 1995), anti-PMSA antibody (J.
- Antibodies that recognize antigens associated with allergy or inflammation include anti-interleukin-6 antibody (Immunol. Rev., 127, 5-24, 1992) and anti-interleukin-6 receptor antibody (Molecular Immunol., 31, 371- 381, 1994), anti-interleukin 5 antibody (Immunol. Rev., 127, 5-24, 1992), anti-interleukin 5 receptor antibody, anti-interleukin 4 antibody (Cytokine, 3, 562-567, 1991) , Anti-interleukin-4 receptor antibody (J. Immunol.
- anti-tumor necrosis factor antibody Hybridoma, 13, 183-190, 1994
- anti-tumor necrosis factor receptor Antibody Molecular Pharmacol., 58, 237-245, 2000
- anti-CCR antibody Nature, 400, 776-780, 1999
- anti-chemokine antibody J. Immunol. Meth., 174, 249-257, 1994
- Anti-chemoforce-in receptor antibody J. Exp.
- Antibodies that recognize antigens associated with cardiovascular diseases include anti-GpIIb / IIIa antibodies (J. Immunol., 152, 2968-2976, 1994) and anti-platelet-derived growth factor antibodies (Science, 253, 1129—1132, 1991). ), Anti-platelet-derived growth factor receptor antibody (J. Biol. Chem., 272, 17400-17404, 1997) or anticoagulation factor antibody (Circulation, 101, 1158-1164, 2000).
- Antibodies that recognize antigens associated with autoimmune diseases include anti-self DNA antibodies
- Antibodies that recognize antigens associated with viral or bacterial infection include anti-gp120 antibody (Structure, 8, 385-395, 2000), anti-CD4 antibody (J. Rheumatology, 25, 2065-2076, 1998), anti-CCR4 Antibodies, anti-verotoxin antibodies (J. Clin.
- autoimmune diseases psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, systemic lupus erythematosus, multiple sclerosis, etc.
- Anti-CDlla antibody, anti-ICAM3 antibody, anti-CD80 antibody, anti-CD2 antibody, anti-CD3 antibody, anti-CD4 antibody, anti-integrin ⁇ ; 4j3 7 antibody, anti-CD40L antibody, anti-IL-2 receptor antibody Immunology Today, 21 (8), 403-410
- the antibody molecule includes any molecule that contains the Fc region of the antibody. Specific examples include an antibody, an antibody fragment, and a fusion protein containing an Fc region.
- the antibody may be any protein that is produced in vivo by an immune reaction as a result of stimulation with a foreign antigen and has an activity of specifically binding to the antigen.
- An antibody secreted by a hybridoma cell prepared from spleen cells of an immunized animal, an antibody prepared by gene recombination technology, that is, an antibody expression vector containing an antibody gene is introduced into host cells. Examples include acquired antibodies. Specific examples include antibodies, humanized antibodies, and human antibodies produced by hybridomas.
- a hybridoma is a monoclonal antibody having a desired antigen specificity, obtained by cell fusion of B cells obtained by immunizing a mammal other than human with an antigen and myeloma cells derived from mice, rats, etc. Refers to cells that produce
- humanized antibody examples include a human chimeric antibody and a human CDR-grafted antibody.
- the human chimeric antibody is composed of a non-human animal H chain V region (hereinafter, also referred to as HV or VH) and an antibody L chain V region (hereinafter, also referred to as LV or VL) and a human antibody H chain C region (hereinafter, also referred to as LV or VL).
- HV or VH non-human animal H chain V region
- LV or VL antibody L chain V region
- LV or VL human antibody H chain C region
- CL human antibody H chain C region
- Non-human animals include mice, rats, and c. Anything can be used as long as it is possible to produce a high-priority dorma, such as a muster or a heron.
- a human chimeric antibody is obtained by obtaining cDNAs encoding VH and VL from a hybridoma producing a monoclonal antibody, and converting the cDNAs into host cell expression vectors having genes encoding human antibody CH and human antibody CL, respectively.
- a human-type chimeric antibody expression vector can be constructed by insertion and expressed and produced by introducing it into a host cell.
- the CH of the human chimeric antibody may be any CH as long as it belongs to human immunoglobulin (hereinafter, referred to as hlg), but is preferably of the hlgG class, and furthermore, hIgGl, hIgG2, Any of the subclasses hIgG3 N hIgG4 can be used.
- the CL of the human chimeric antibody may be any CL as long as it belongs to hlg, and a ⁇ class or L class can be used.
- the human CDR-grafted antibody refers to an antibody obtained by grafting the amino acid sequence of CDRs of VH and VL of an antibody of a non-human animal to an appropriate position of VH and VL of a human antibody.
- the human CDR-grafted antibody is constructed by constructing a cDNA encoding the V region obtained by grafting the VH and VL CDR sequences of a non-human animal antibody to the VH and VL CDR sequences of a desired human antibody.
- the human CDR-grafted antibody expression vector is constructed by inserting the expression vector into a host cell expression vector having the genes encoding the CH and the human antibody CL, and the expression vector is introduced into the host cell.
- the transplant antibody can be expressed and manufactured.
- the CH of the human CDR-grafted antibody may be any CH as long as it belongs to hlg, but the hlgG class is preferable, and any subclass such as hIgGl, hIgG2, gG3, hIgG belonging to the hlgG class may be used. it can.
- the CL of the human-type CDR-transplanted antibody may be any CL as long as it belongs to hlg, and may be of the ⁇ class or the ⁇ ⁇ ⁇ class.
- a human antibody originally refers to an antibody naturally occurring in a human body.
- a human antibody phage library and a human antibody phage library produced by recent advances in genetic, cell engineering, and developmental engineering techniques have been developed. Also included are antibodies obtained from transgenic non-human animals producing human antibodies or transgenic plants producing human antibodies.
- Antibodies present in the human body can be obtained, for example, by isolating human peripheral blood lymphocytes, infecting EB virus and the like, immortalizing them, and cloning the cells to produce the antibody-producing lymphocytes.
- the antibody can be further purified.
- the human antibody phage library 1 is a library in which antibody fragments such as Fab and single-chain antibodies are expressed on the phage surface by inserting an antibody gene prepared from human B cells into the phage gene. From the library, phage expressing an antibody fragment having the desired antigen-binding activity can be recovered using the binding activity to the substrate on which the antigen is immobilized as an index.
- the antibody fragment can be further converted to a human antibody molecule consisting of two complete H chains and two complete L chains by genetic engineering techniques.
- the human antibody-producing transgenic non-human animal refers to a non-human animal in which a human antibody gene has been integrated into cells.
- a human antibody-producing transgenic animal can be produced by introducing a human antibody gene into mouse embryonic stem cells, transplanting the embryonic stem cells into an early embryo of another mouse, and then developing the embryonic stem cells. it can.
- a transgenic non-human animal that produces a human antibody can be produced by introducing a human antibody gene into a fertilized egg of an animal and generating the fertilized egg.
- a method for producing a human antibody from a human antibody-producing non-transgenic nonhuman animal is performed by obtaining a human antibody-producing hybridoma by a conventional method for producing a hybridoma in a mammal other than a human, and culturing the antibody. Human antibodies can be produced and accumulated in the culture.
- transgenic non-human animals examples include porcupines, sheep, goats, goats, stags, pomas, mice, rats, chickens, monkeys, and egrets.
- the antibody may be an antibody that recognizes a tumor-associated antigen, an antibody that recognizes an allergy or an inflammation-related antigen, an antibody that recognizes a circulatory disease-related antigen, an autoimmune disease-related antigen
- the antibody is an antibody that recognizes an antigen, or an antibody that recognizes an antigen associated with a virus or bacterial infection.
- a human antibody whose antibody class is IgG is preferable.
- the antibody fragment refers to a fragment containing at least a part of the Fc region of the antibody.
- the Fc region means a C-terminal region of the H chain of an antibody, a CH2 region and a CH3 region, and includes a natural type and a mutant type thereof.
- At least a part of the Fc region preferably refers to a fragment containing the CH2 region, more preferably a region containing the first aspartic acid present in the CH2 region.
- antibody fragments include H chain monomers and H chain dimers.
- a fusion protein having a part of the Fc region is a substance obtained by fusing an antibody or a fragment of an antibody containing a part of the Fc region of an antibody with a protein such as an enzyme or cytokin (hereinafter referred to as Fc Fusion protein).
- the cells of the present invention can be prepared by the method described below.
- the cells of the present invention can be prepared by targeting the gene for the ⁇ 1,6-fucose modifying enzyme and using a gene disruption method.
- Examples of the enzyme in which the 1-position of fucose is ⁇ -linked to the 6-position of ⁇ -glycidyl-linked glycan at the 6-position of ⁇ -glycoside-linked complex type sugar chain are specifically al, 6-fucosyltransferase, Fucosidase and the like.
- the method for gene disruption includes any method capable of disrupting the gene of the target enzyme. Examples thereof include a homologous recombination method, an RNA-DNA oligonucleotide (RDO) method, a method using retrovirus / less, a method using transposon, and the like. Hereinafter, these will be described in detail.
- RDO RNA-DNA oligonucleotide
- the cells of the present invention can be prepared by targeting the gene of the 1,6-fucose modifying enzyme and modifying the target gene on the chromosome using the homologous recombination method.
- Modification of the target gene on the chromosome can be performed using the method described in the Manipulating the Mouse Embryo A Laboratory Manual, Second Edition, Cold Spring Harbor laboratory Press (1994) (hereinafter, ⁇ manipulating the mouse Embryo A laboratory laboratory ''). Yuanore ”), Gene Targeting, A Practical Approach, IRL Press at Oxford University Press (1993), Biomanual Series 8 Gene Targeting, Production of Mutant Mice Using ES Cells, Yodosha (1995) (hereinafter The method can be performed as follows, for example, by using the chromosome engineering technique described in “Generation of mutant mouse using ES cells”). a Obtain cDNA of 1,6-fucose modifying enzyme.
- a genomic DNA of al, 6-fucose modifying enzyme is prepared.
- a target vector for homologous recombination of a target gene to be modified eg, a structural gene of al, 6-fucose modifying enzyme or a promoter gene.
- the host cell of the present invention can be prepared by introducing the prepared target vector into a host cell and selecting a cell in which homologous recombination has occurred between the target gene and the target vector.
- any of yeast, animal cells, insect cells, plant cells, and the like can be used as long as it has the gene of the target a1,6-fucose modifying enzyme.
- Specific examples include the cells described in 3. below.
- Methods for obtaining cDNA and genomic DNA of a 1,6-fucose modifying enzyme include, for example, the methods described below.
- a cDNA library is prepared from the prepared total RNA or mRNA.
- a degenerative primer was prepared. Obtain a gene fragment encoding the 1,6-fucose modifying enzyme.
- the cDNA library can be screened to obtain cDNA encoding a1,6-fucose modifying enzyme.
- MRNA of various host cells may be commercially available (for example, manufactured by Clontech), or may be prepared from various host cells as described below.
- Methods for preparing total RNA from various host cells include guanidine thiocyanate-cesium trifluoroacetate method [Methods in Enzymology, 154, 3 (1987)], guanidine acid thiocyanate. Phenol 'Cloth Holm (AGP C) method [Analytical' Analytical Biochemistry, 162, 156 (1987); Experimental Medicine, 9, 1937 (1991)].
- AGP C Phenol 'Cloth Holm
- mRNA as poly (A) + RNA from total RNA an oligo (dT) -immobilized cellulose column method (Molecular Clawing, 2nd edition) and the like can be mentioned.
- mRNA can be prepared by using a kit such as Fast Track mRNA Isolation Kit (Invitrogen) or Quick Prep mRNA Purification Kit (Pharmacia).
- kit such as Fast Track mRNA Isolation Kit (Invitrogen) or Quick Prep mRNA Purification Kit (Pharmacia).
- CDNA libraries are prepared from the prepared host cell mRNAs.
- Methods for preparing a DNA library include the methods described in Molecular 'Cloning 2nd Edition, Current-Protocols in Molecular-Norology, A Laboratory Manual, 2nd Ed. (1989), or Examples thereof include a method using a commercially available kit, for example, a Superscript Plasmid System ior cDNA Synthesis and Plasmia Coating (manufactured by Life Technologies) and a ZAP-cDNA Synthesis Kit (manufactured by STRATAGENE).
- any phage vector, plasmid vector, or the like can be used as long as it can replicate autonomously in the Escherichia coli K12 strain.
- ZAP Express [Stratagies, Strategies, 5, 58 (1992)], pBluescript II SK (+) [Nucleic Acids Research], 17, 9494 (1989)], Lambda ZAP II (manufactured by STRATAGENE); LgtlO, ⁇ gtll [DNA cloning, A practical approach], 1, 9 (1985)], XTriplEx (Clontech), ExCell (Pharmacia), pT7T318U (Pharmacia), pcD2 [Molecular 'Cellular' Biology (Mol. Cell. Biol.), 3, 280 (1983)] and pUC18 [Gene (Gene ), 33, 103 (1985)].
- Escherichia coli As a host microorganism for producing a cDNA library, any microorganism can be used, but Escherichia coli is preferably used. Specifically, Escherichia coli XL1-Blue MRF '[Stratagies, 5, 81 (1992)], Escherichia coli C600 [Genetics, 39, 440 (1954)] , Escherichia coli Y1088 [Science, 222, 778 (1983)], Escherichia coli Y1090 [Science, 222, 778 (1983)], Escherichia coli NM522 [Giananole 'ob. (J. Mol.
- This cDNA library may be used as is for subsequent analysis, but in order to reduce the proportion of incomplete-length cDNA and obtain full-length cDNA as efficiently as possible, the oligocap method developed by Sugano et al. [Gene ( Gene), 138, 171 (1994); Gene, 200, 149 (1997); Protein nucleic acid enzyme, 41, 603 (1996); Experimental medicine, 11, 2491 (1993); (1996); Production of Gene Library (Yodosha) (1994)].
- the fact that the obtained gene fragment is DN ⁇ encoding ⁇ 1,6-fucose modifying enzyme can be determined by a commonly used nucleotide sequence analysis method, for example, the dideoxy method of Sanger et al. Using a base sequence analyzer such as 'National' Academy 'of Science' (Proc. Natl. Acad. Sci. USA), 74, 5463 (1977)] or AB IPRIS M377 DNA Sequencer (manufactured by PE Biosystems). Can be confirmed by analysis.
- cDNA or a cDNA library pair synthesized from mRNA contained in various host cells is subjected to colony hybridization and plaque hybridization (molecular cloning second edition). al, DNA of 6-fucose modifying enzyme can be obtained.
- a cDNA or cDNA library synthesized from mRNA contained in various host cells was used as a By performing screening using the PCR method, the DNA of al, 6-fucose modifying enzyme can also be obtained. From the end, the nucleotide sequence of D ⁇ encoding the obtained 1,6-fucose modifying enzyme was analyzed from the end by a commonly used nucleotide sequence analysis method, for example, the didoxy method of Sanger et al. National 'Academy' of Science '(Proc. Natl. Acad. Sci. USA), 74, 5463 (1977)] or AB IPRI SM377D NA Sequencer (manufactured by PE Biosystems). By doing so, the nucleotide sequence of the DNA is determined.
- a base sequence database such as GenBank, EMBL, and DDBJ is searched using a homology search program such as BLAST, so that the database Among the genes, the gene encoding the a 1,6-fucose modifying enzyme can also be determined.
- the nucleotide sequence of the gene encoding the 1,6-fucose modifying enzyme obtained by the above method includes, for example, the nucleotide sequence of SEQ ID NO: 1 or 2.
- Enzyme cDNA can also be obtained.
- Examples of a method for preparing genomic DNA of ⁇ 1,6-fucose modifying enzyme include the following methods. Preparation of Genome DN II
- genomic DNA of c ⁇ l, 6-fucose modifying enzyme can be isolated by using a genomic DNA library screening system (Genome Systems) or Universal GenomeWalker TM Kits (CL0NTECH).
- the base sequence of the genomic DNA of the ⁇ 1,6-fucose modifying enzyme obtained by the above method includes, for example, the base sequence of SEQ ID NO: 3.
- Target vectors for homologous recombination of the target gene are as follows: Gene Targeting, A Practical Approach, IRL Press at Oxford University Press (1993), Neu-Shi Manual Manual Series 8 Gene Targeting, Generation of Mutant Mice Using ES Cells ( (Yodosha) (19%).
- Target The cut vector can be used in either a replacement type or an insertion type.
- the method for introducing a recombinant vector suitable for various cells described in 3. below can be used.
- Examples of methods for efficiently selecting homologous recombinants include, for example, Gene Targeting, A Practical Approach, IRL Press at Oxford University Press (1993), Nozuma-manual series 8 Gene targeting, and mutant mouse using ES cells. For example, methods such as positive selection, promoter selection, negative selection, and poly A selection described in Production (Yodosha) (1995) can be used. Methods for selecting the desired homologous recombinant from the selected cell lines include the Southern hybridization method for genomic DNA (molecular cloning, second edition) and the PCR method [PCR Protocols (PCR Protocols)]. ), Academic Press (1990)].
- homologous recombinants were obtained by using as an index the change in enzyme activity related to sugar chain modification in which the 6-position of N-acetyldarcosamine at the reducing end of the N-daricoside-linked sugar chain and the 1-position of fucose are ⁇ -linked. You can also. As a specific method, for example, a method for selecting a transformant described below can be mentioned. How to select transformants
- Biochemical methods include, for example, a method of evaluating enzyme activity using an enzyme-specific substrate.
- Examples of the genetic engineering method include Northern analysis for measuring the amount of mRNA of an enzyme gene and the RT-PCR method.
- Methods for selecting cells based on changes in traits resulting from reduced activity of a 1,6-fucose modifying enzyme include, for example, selecting transformants using the bran chain structure of a produced antibody molecule as an index. And a method of selecting a transformant using the sugar chain structure of a glycoprotein on the cell surface as an index. Examples of a method for selecting a transformant using the sugar chain structure of the produced antibody molecule as an index include the method described in 5. below.
- a method for selecting a transformant using the sugar chain structure of a glycoprotein on the cell surface as an index is as follows. The 6-position of N-acetyldarcosamine at the reducing end of the N-glycoside-linked sugar chain and the 1-position of fucose are ⁇ -linked.
- a method for selecting a strain that is resistant to a lectin that recognizes a sugar chain structure can be mentioned. Specific examples thereof include a method using a lectin described in Somatake 'Cell' and 'Molecular' Genetics, 12, 51 (1986).
- any lectin can be used as long as it recognizes a sugar chain structure in which the 6-position of N-acetyldarcosamine at the reducing end of the N-daricoside-linked sugar chain and the 1-position of fucose are ⁇ -linked.
- Lentil Lectin LCA Lientil Agglutinin from Lens Culinaris
- Enduma Lectin PSA Pea Lectin from Pi sum sativum
- Broad Bean Lectin VFA Adgglutinin from Vicia faba
- AAL Aleuriaur from Aloiaur Lectin
- Cells can be selected.
- the cells of the present invention can be prepared as follows, for example, using the RDO method, targeting the gene of the 1,6-fucose modifying enzyme.
- the base sequence of the prepared cDNA or genomic DNA is determined. Based on the determined DNA sequence, design and synthesize an RDO construct of an appropriate length, including the portion encoding the al, 6-fucose modifying enzyme, the untranslated region, or the intron.
- the synthesized RDO into a host cell enzymes that target, i.e. by selecting a transformant mutations in alpha 1,6-fucose modifying enzyme produced can Seisuru cells action of the present invention .
- any of yeast, animal cells, insect cells, plant cells, and the like can be used as long as it has the gene of the target ⁇ 1,6-fucose modifying enzyme.
- Specific examples include the host cells described in 3. below.
- Examples of a method for preparing cDNA of the ⁇ , 6-fucose modifying enzyme include the “method for preparing cDNA” described in (a) of (1) above.
- the DNA sequence of DNA is digested with an appropriate restriction enzyme and the like, cloned into a plasmid such as pBluescript SK (-) (manufactured by Stratagene), and analyzed by a commonly used nucleotide sequence analysis method, for example, dideoxy by Sanger et al. Method [Proceding of the National Academy of Sciences (Proc. Natl. Acad. Sci., USA), 74, 5463 (1977)]
- the base sequence of the DNA can be determined by analysis using ALF DNA Sequencer (Pharmacia) or the like.
- RDO can be prepared by a conventional method or by using a DNA synthesizer.
- Methods for introducing RDO into host cells and selecting cells in which the gene for the targeted enzyme, ⁇ 1,6-fucose modifying enzyme, has been mutated include molecular 'cloning second edition, current' protocols 'in'. Examples include a method for directly detecting a mutation of a gene on a chromosome described in Molecular 'Biology and the like.
- the cells of the present invention can be prepared by using the transposon system described in Nature Genet., 25, 35, (2000), etc., for the activity of ⁇ 1,6-fucose modifying enzyme, or for the production of antibody molecules or on the cell membrane. By selecting a mutant using the sugar chain structure of the glycoprotein as an index, the cell of the present invention can be produced.
- the transposon system is a system in which a foreign gene is randomly introduced into a chromosome to induce mutation.
- a foreign gene contained in a transposon is used as a vector to induce mutation, and this gene is At the same time, a transposase expression vector for randomly introducing chromosomes into the chromosome is introduced into cells.
- transposase Any transposase can be used as long as it is suitable for the sequence of the transposon to be used.
- any gene can be used as long as it can induce mutation in the DNA of a cell.
- any of yeast, animal cells, insect cells, plant cells, and the like can be used as long as they have the gene of the targeted ⁇ 1,6_fucose modifying enzyme.
- Specific examples include the host cells described in 3. below.
- the method for introducing a recombinant vector suitable for various host cells described in 3. below can be used.
- a method for selecting a mutant using the activity of ⁇ 1,6-fucose modifying enzyme as an index for example, a method for changing the activity of al, 6 -fucose modifying enzyme described in (a) of (1) above is used. "A method for selecting a transformant" is used as an index.
- the cells of the present invention target (3 ⁇ 41,6-fucose-modifying enzyme-encoding gene, cell engineering, 12, 239, (1993), bio-Z technology (BI0 / TECHN0L0GY), 17, 1097, ( 1999), Hum. Mol. Genet., 5, 1083, (1995), Cell Engineering, 13 ⁇ 4 255, (1994), Proceedings 'Ob the National' Academia. Natl. Acad. Sci. USA, 96, 1886 (1999) and the like, using the antisense method or the ribozyme method, for example, can be prepared as follows.
- the nucleotide sequence of the prepared cDNA or genomic DNA is determined.
- an antisense gene or ribozyme construct of an appropriate length including a translated region, untranslated region or intron portion of the target gene is designed.
- a recombinant vector is prepared by inserting the prepared DNA fragment or full length downstream of the promoter of an appropriate expression vector.
- a transformant is obtained by introducing the recombinant vector into a host cell suitable for the expression vector.
- the cells of the present invention can be obtained by selecting a transformant using the activity of the protein encoded by the target gene as an indicator.
- the cells of the present invention can also be obtained by selecting a transformant using the sugar chain structure of the glycoprotein on the cell membrane or the sugar chain structure of the produced antibody molecule as an index.
- any cell having a target gene such as yeast, animal cells, insect cells, and plant cells
- yeast, animal cells, insect cells, and plant cells can be used.
- specific examples include the host cells described in 3. below.
- an expression vector it can replicate autonomously in the above host cells or can be integrated into the chromosome, and transcribe the designed antisense gene or ribozyme. Those containing a promoter at the position where they can be used are used.
- Specific examples include the expression vectors described in 3. below.
- the method for introducing a gene into various host cells As a method for introducing a gene into various host cells, the method for introducing a recombinant vector suitable for various host cells described in the following section 3 can be used.
- Methods for obtaining cDNA and genomic DNA of the target gene include, for example, “method for preparing cDNA” and “method for preparing genomic DNA” described in (a) of (1) above. Is raised.
- Examples of a method for selecting a transformant using the activity of the protein encoded by the target gene as an index include the “method for selecting a transformant” described in (a) of (1) above.
- Methods for selecting cells based on changes in traits resulting from reduced activity of the protein encoded by the target gene include, for example, selecting transformants using the sugar chain structure of the produced antibody molecule as an index, and selecting a transformant.
- a method of selecting a transformant using the sugar chain structure of a glycoprotein on the cell surface as an index is exemplified. Examples of a method for selecting a transformant using the sugar chain structure of the produced antibody molecule as an index include the method described in 5. below.
- the cells of the present invention can also be obtained by directly introducing an antisense oligonucleotide or lipozyme designed based on the base sequence of the target gene without using an expression vector into a host cell.
- the antisense oligonucleotide or ribozyme can be prepared by a conventional method or by using a DNA synthesizer. Specifically, of the base sequences of the cDNA and genomic DNA of the target gene, a sequence corresponding to 5 to 150 consecutive bases, preferably 5 to 60 bases, and more preferably 10 to 40 bases. It can be prepared by synthesizing an oligonucleotide (antisense oligonucleotide) corresponding to a sequence complementary to the oligonucleotide or a ribozyme containing the sequence of the oligonucleotide based on the sequence information of the oligonucleotide. Oligonucleotides include oligo RNAs and derivatives of the oligonucleotides (hereinafter referred to as oligonucleotide derivatives).
- oligonucleotide derivative examples include an oligonucleotide derivative in which a phosphodiester bond in an oligonucleotide is converted to a phosphorothioate bond, and a phosphodiester bond in an oligonucleotide in which N 3′—P 5 ′ phosphoramidate is used.
- Oligonucleotide derivative converted to bond Oligonucleotide derivative in which report and phosphodiester bond in oligonucleotide are converted to peptide nucleic acid bond, and peracyl in oligonucleotide substituted with C-15 propynyl peracyl
- Oligonucleotide derivatives Oligonucleotide derivatives, oligonucleotide derivatives in which peracyl in oligonucleotides are substituted with C-15 thiazole peracyl, oligonucleotide derivatives in which cytosines in oligonucleotides are substituted with C-15 propynylcytosine, oligonucleotides
- Oligonucleotide derivative in which cytosine in the product is replaced with phenoxazine-modified cytosine
- the cells of the present invention can be prepared using the RNAi (RNA interference) method targeting the gene encoding the protein of the present invention, for example, as follows.
- the RNAi method is a method in which double-stranded RNA is introduced into cells, and mRNA homologous to the RA sequence present in the cells is degraded and broken to suppress gene expression.
- the base sequence of the prepared cDNA is determined.
- ⁇ which is a part of the translation region of the target gene, designs an RNAi gene construct of an appropriate length including a part of the untranslated region.
- a recombinant vector is prepared by inserting the prepared DNA fragment or its full length downstream of the promoter of an appropriate expression vector.
- a transformant is obtained by introducing the recombinant vector into a host cell suitable for the expression vector.
- the cells of the present invention can be obtained by selecting a transformant based on the activity of the protein encoded by the target gene or the sugar chain structure of the produced antibody molecule or the glycoprotein on the cell surface.
- any of yeast, animal cells, insect cells, plant cells, and the like that have the target gene can be used. Specific examples include the host cells described in 3. below.
- the expression vector those which can replicate autonomously in the above-mentioned host cells or can be integrated into the chromosome, and which contain a promoter at a position where the designed RNAi gene can be transcribed are used.
- Specific examples include an expression vector of a type in which transcription is performed by polymerase III or an expression vector described in 3. below.
- Examples of a method for selecting a transformant using the activity of the protein encoded by the target gene as an index or a method for selecting a transformant using the sugar chain structure of a glycoprotein on a cell membrane as an index include, for example, the method described in the above 1 ( The method described in (a) of 1) can be used.
- Examples of a method for selecting a transformant using the sugar chain structure of the produced antibody molecule as an index include the method described in 5. below.
- Examples of the method for preparing cDNA of the protein encoded by the target gene include the “method for preparing cDNA” described in (1) (a) above.
- the cells of the present invention can also be obtained by directly introducing the RNAi gene designed based on the base sequence of the target gene without using an expression vector into a host cell.
- the RNAi gene can be prepared by a conventional method or by using a DNA synthesizer.
- RNAi gene construct is described in [Nature, 391, 806, (1998); Proc. Natl. Acad. Sci. USA), 95, 15502, (1998); Nature, 395, 854, (1998); Proc. Natl. Acad. Sci. USA), 96, 5049, (1999); Cell, 95, 1017, (1998); Proceedings' of the National Academy of Sciences (Proc. Natl. Acad. Sci. USA), 96, 1451, (1999); The 'National' Academy 'of Science (Proc. Natl. Acad. Sci. USA), 95, 13959, (1998); Nature's Cell's Pyology (Nature Cell Biol.), 2, 70, (2000)] Etc. can be designed.
- the RA used in the RNAi method of the present invention includes an enzyme involved in sugar chain modification in which the 1-position of fucose is ⁇ -linked to the 6-position of N-acetyldarcosamine at the reducing terminal of N-dalicoside-linked complex type sugar chain.
- RNA corresponding to the DNA encoding the protein can be mentioned.
- New - grayed glycosidic bond in a complex type sugar chain enzyme proteins involved in the 1-position force S alpha binding to sugar chain modification of ⁇ - the 6-position of ⁇ cetyl Darco Sa Min fucose reducing end, shed 1, 6 - fucosyl transflector RNA corresponding to DNA that is erase is included.
- RNA used in the RNAi method of the present invention is a double-stranded RNA that is composed of RNA and its phase capture RNA and is capable of reducing the mRNA amount of al, 6-fucosyltransferase. Any length may be used as long as the length of the RNA is preferably 1 to 30, more preferably 5 to 29, further preferably 10 to 29, and most preferably 15 to 29. RNA.
- the cells of the present invention are prepared by targeting a 1,6-fucose modifying enzyme and using a technique of introducing a dominant negative body of the protein. Can be made.
- a specific example of a dominant negative body is an example of a protein involved in transport of the intracellular sugar nucleotide GDP-fucose to the Golgi apparatus.
- the intracellular sugar nucleotide transporter functions by forming a dimer on the membrane of the endoplasmic reticulum and the Golgi apparatus [Journal of Biological 'Chemistry (J. Biol. Chem.), 275, 17718, 2000].
- intracellular sugar nucleotide transporter mutants by forcibly expressing intracellular sugar nucleotide transporter mutants in cells, they form heterodimers with wild-type transporters, and the formed heterodimers exhibit inhibitory activity against wild-type homodimers. [Journal of Biological 'Chemistry (J. Biol. Chem.), 275, 17718, 2000]. Therefore, by producing a mutant of the intracellular sugar nucleotide transporter and introducing it into the cell, it is possible to function as a dominant negative form. The generation of such mutants is described in Molecular 'Cloning 2nd Edition, Current
- the cells of the present invention were prepared using the dominant-negative gene of the 1,6-fucose modifying enzyme prepared as described above, using molecular 'Clawing 2nd ed., Current'protocols' in 'Molekiura' ⁇ biology, According to the method for gene transfer described in Manipulating, Mouse, and Embryo, 2nd edition, for example, it can be prepared as follows.
- a DNA fragment having an appropriate length containing a portion encoding the protein is prepared.
- a recombinant vector is prepared.
- a transformant is obtained by introducing the recombinant vector into a host cell suitable for the expression vector.
- the host cells of the present invention can be prepared by selecting transformants based on the activity of the 1,6-fucose modifying enzyme or the sugar chain structure of the produced antibody molecule or the glycoprotein on the cell membrane.
- any of yeast, animal cells, insect cells, plant cells, and the like can be used as long as they have the gene of the target protein. Specific examples include the host cells described in 3. below.
- the expression vector those which can replicate autonomously in the above-mentioned host cells or can be integrated into the chromosome, and which contain a promoter at a position where DNA encoding the dominant negative body of interest can be transcribed are used. Specific examples include the expression vectors described in 3. below.
- the method for introducing a recombinant vector suitable for various host cells described in 3. below can be used.
- a method for selecting a mutant using the activity of the target protein as an index or a method for selecting a mutant using the sugar chain structure of a glycoprotein on a cell membrane as an index, for example, the method described in the above 1 (1) Method.
- a method for selecting a transformant using the sugar chain structure of the produced antibody molecule as an index include the method described in 4. below.
- the cells of the present invention can be prepared by introducing a mutation into the gene of the 1,6-fucose modifying enzyme and selecting a desired cell line in which the enzyme has been mutated.
- Examples of the enzyme in which the 1-position of fucose is ⁇ -linked to the 6-position of N-acetyldarcosamine at the reducing end of the N-daricoside-linked complex type sugar chain specifically, 1,6-fucosyltransferase, and -L-fucosidase and the like.
- mutants obtained by treating the parent cell line in the mutagenesis treatment or from naturally occurring mutants using the change in ⁇ 1,6-fucose modifying enzyme activity as an index
- a desired cell line from the mutant or spontaneously generated mutant obtained by treating the parent cell line by mutagenesis using the sugar chain structure of the produced antibody molecule as an index.
- a desired structure is obtained by using the sugar chain structure of a glycoprotein on the cell membrane of the cell as an index. Examples include a method for selecting a cell line.
- any treatment can be used as long as it induces a point mutation, a deletion or a frame shift mutation in the DNA of the parent cell line.
- Specific examples include treatment with ethyl ditrosperia, nitrosoguanidine, benzopyrene, and atharidin dye, and irradiation with radiation.
- various alkylating agents and carcinogens can also be used as mutagens. Examples of the method of causing a mutagen to act on cells include, for example, the third edition of tissue culture technology (Asakura Shoten), edited by The Japanese Society for Tissue Culture (1996), Nature Genet., 24, 314, (2000).
- Spontaneously occurring mutants include those spontaneously generated by continuing subculture under normal cell culture conditions without special mutagenesis treatment. .
- a method for selecting a desired cell line using the change in ⁇ 1,6-fucose modifying enzyme activity as an index a method for selecting a desired cell line using the sugar chain structure of a produced antibody molecule as an index, a sugar chain of a glycoprotein on a cell membrane
- a method for selecting a desired cell line using the structure as an index for example, the method described in (1) (1) (a) above, which is based on the change in ⁇ 1,6-fucose modifying enzyme activity described in (1) above, is used.
- Method for selecting transformants ”.
- the host cell of the present invention targets an ⁇ , 6-fucose modifying enzyme gene and uses antisense RNA / DNA technology [Bioscience and Industry, 50, 322 (1992), Chemistry,, 681 (1991), Biotechnology, 9, 358 (1992), Trends in Biotechnology, 10, 87 (1992), Trends in Biotechnology, 10, 152 (1992), Cell Engineering, l ⁇ , 1463 (1997)], triple helicopter It can be prepared by suppressing the transcription or translation of a target gene using the X-ray technology [Trends in Biotechnology, 10, 132 (1992)] and the like.
- the transgenic non-human animal or plant or the progeny thereof, in which the genomic gene is modified so that the activity of the enzyme involved in the modification of the sugar chain of the antibody molecule of the present invention is controlled, is an ⁇ 1,6-fucose modifying enzyme
- the gene can be produced from the embryonic stem cell, fertilized egg cell, or plant cell prepared by the method described in 1. using a known method.
- Examples of an enzyme in which the 1-position of fucose is ⁇ -linked to the 6-position of ⁇ ⁇ -acetyldarcosamine at the reducing end of ⁇ -glycoside-linked complex-type sugar chain include a 1,6-fucosyltransferase, a _L_fucosidase and the like.
- the target non-human animals for example, embryonic stem cells such as porcupines, sheep, goats, stags, pomas, mice, rats, chicks, monkeys, and egrets, are described in 1.
- embryonic stem cells of the present invention in which the activity of al, 6-fucose modifying enzyme is controlled can be produced.
- a gene encoding al, 6-fucose modifying enzyme on a chromosome can be obtained by a known homologous recombination technique [eg, Nature, 326, 6110, 295 (1987), Cell, 51, 3, 503 ( 1987) etc.], and a mutant clone inactivated or substituted with an arbitrary sequence.
- a mutant clone inactivated or substituted with an arbitrary sequence.
- embryonic stem cells for example, the mutant clone
- a chimeric individual consisting of a sex stem cell clone and normal cells by a technique such as injection chimera method or assembly chimera method of injecting animal fertilized eggs into blastocysts. Can be prepared.
- a transgenic non-human animal having reduced a1,6-fucose modifying enzyme activity in whole body cells By crossing the chimeric individual with a normal individual, a transgenic non-human animal having reduced a1,6-fucose modifying enzyme activity in whole body cells can be obtained.
- the target vector for homologous recombination of the target gene is prepared according to the method described in Gene Targeting, A Practical Approach, IRL Press at Oxford University Press (1993), Production of Mutant Mouse Using ES Itoda Tsukiha, etc. be able to.
- As the target vector any of a replacement type, an insertion type, and a gene trap type can be used.
- any method for introducing a target vector into embryonic stem cells any method can be used as long as it introduces DNA into animal cells.
- electroporation method Japanese Unexamined Patent Application Publication No. 2-227075
- riboxoxy method procedures 'ob''the' national 'akademiobu' science (Proc. Natl. Acad. Sci. USA) , 84, 7413 (1987)]
- injection method manipulating 'mouse' Employ second edition
- the method using a particle gun (gene gun) Japanese Patent No.
- Methods for efficiently selecting homologous recombinants include, for example, positive selection and promoter described in Gene Targeting, A Practical Approach, IRL Press at Oxford University Press (1993), production of mutant mice using ES Itoda vesicle, etc. Methods such as selection, negative selection, and poly A selection can be used. Specifically, in the case of a target vector containing the hprt gene, after introduction into the embryonic stem cells lacking the hprt gene, the embryonic stem cells are cultured in a medium containing aminopterin, hypoxanthine and thymidine, and aminobuterin is obtained. By selecting resistant strains, positive selection for selecting homologous recombinants containing the hprt gene can be performed.
- a target vector containing the neomycin resistance gene embryo-derived stem cells into which the vector has been introduced are cultured in a G418-containing medium, and G418-resistant strains are selected to select homologous recombinants containing the neomycin resistance gene. Positive selection can be performed.
- a target vector containing the DT gene embryonic stem cells into which the vector has been introduced are cultured, and the growing strain is selected. (Recombinants randomly inserted into the chromosome other than homologous recombination have the DT gene in the chromosome.
- embryonic stem cells When embryonic stem cells are incorporated into a fertilized egg by using the chimeric assembly method, it is generally preferable to use a fertilized egg at a developmental stage before the 8-cell stage. When embryonic stem cells are incorporated into a fertilized egg using the injection chimera method, it is generally preferable to use a fertilized egg from the 8-cell stage to the stage of blastocyst development.
- LHRH fertilizing hormone-releasing hormone
- the method described in 1 above should be used for fertilized egg cells of the target non-human animal, for example, a porcupine, a sheep, a goat, a pig, a poma, a mouse, a rat, a chicken, a monkey, a rabbit, etc.
- the fertilized egg cell of the present invention in which the activity of the 1,6-fucose modifying enzyme has been reduced can be produced.
- the resulting fertilized egg cells are transplanted into the oviduct or uterus of a pseudopregnant female using the method of embryo transfer described in Manipulating 'Mouse' Embrio, 2nd edition, etc. to give birth to 1,6-fucose.
- Transgenic non-human animals with reduced activity of the modifying enzyme can also be produced.
- the target plant virulent or cells are subjected to the method described in 1 above to reduce the activity of the ⁇ 1,6-fucose modifying enzyme to the virulent plant comprising the plant cells of the present invention. Can be produced.
- the prepared callus is subjected to auxin and cytokinin according to a known method [tissue culture, 20 (1994); tissue culture, 21 (1995); Trends in Biotechnology, 15, 45 (1997)].
- auxin and cytokinin according to a known method [tissue culture, 20 (1994); tissue culture, 21 (1995); Trends in Biotechnology, 15, 45 (1997)].
- tissue culture, 20 (1994); tissue culture, 21 (1995); Trends in Biotechnology, 15, 45 (1997) By culturing the cells in a medium containing, the transgenic plants having reduced ⁇ 1,6-fucose modifying enzyme activity can be produced. 3. Production method of antibody composition
- Antibody compositions are described in Molecular 'Cloning 2nd Edition, Current' Protocols' in * Mole and DuPont-Iron, Antibodies, A Laboratory manual, Cold Spring Harbor Laboratory, 1988 (hereinafter abbreviated as Antibodies). , Monoclonal Antibodies: principles and practice, Third Edition, Acad. Press, 1996 (hereinafter abbreviated as monoclonal 'antibodies'), Antibody Engineering, A Practical Approacn, IRL Press at Oxford University Press, 1996 (hereafter T, Antibody) The method can be obtained by expressing in a host cell as described below, for example.
- a DNA fragment of an appropriate length including a portion encoding the protein is prepared.
- a recombinant vector is prepared by inserting the DNA fragment or full-length cDNA into the downstream of the promoter of an appropriate expression vector.
- a transformant producing the antibody composition By introducing the recombinant vector into a host cell suitable for the expression vector, a transformant producing the antibody composition can be obtained.
- Any host cell can be used as long as it can express the gene of interest, such as yeast, animal cells, insect cells, plant cells, and the like.
- Enzymes involved in the modification of N-glycoside-linked sugar chains that bind to the Fc region of an antibody molecule are introduced using genetic engineering techniques.
- Cells such as yeast, animal cells, insect cells, and plant cells are used as host cells. It can also be used.
- Examples of the host cells used in the method for producing the antibody composition include the cells of the present invention prepared in 1. above.
- An expression vector that is capable of autonomous replication in the above-described various host cells or that can be integrated into a chromosome and that contains a promoter at a position where the DNA encoding the antibody molecule of interest can be transcribed is used. .
- cDNA can be obtained from a human or non-human animal tissue or cell by a probe specific to the antibody molecule of interest. It can be prepared using primers.
- yeast When yeast is used as a host cell, examples of expression vectors include YEP13 (ATCC37115), YEp24 (ATCC37051), and YCp50 (ATCC37419).
- the promoter any promoter can be used as long as it can be expressed in yeast strains.
- Examples include the PHO5 promoter, PGK promoter, GAP promoter, ADH promoter, gal 1 promoter, gal 10 promoter, heat shock protein promoter, MFal promoter, CUP 1 promoter and the like.
- yeast belonging to the genera Saccharomyces, Schizosaccharomyces, Krybetella genus, Trichosporon, Schuniomyces, and the like. Can s power.
- any method can be used as long as it is a method for introducing DNA into yeast.
- an electroporation method [Methods. Enzymol., 194, 182 (1990)]
- the Spheroplast method [Proc. Natl. Acad. Sci. USA], 84, 1929 (1978)]
- acetic acid Lithium method [Journal of Bacteriology (J. Bacteriology), 153, 163 (1983)], Proceedings' Ob 'The National' Academy 'Ob' Science (Proc. Natl. Acad. Sci. USA), 75, 1929 (1978)].
- examples of expression vectors include pcDNAI, pcDM8 (commercially available from Funakoshi), pAGE107 [JP-A-3-22979; Cytotechnology, 3, 133, (1990). )], PAS3-3 [Japanese Unexamined Patent Publication No. 2-227075], PCDM8 [Nature, 329, 840, (1987)], pcDNAI / Amp (manufactured by Invitrogen), pREP4 (manufactured by Invitrogen), pAGE103 [journal Biochemistry, 101, 1307 (1987)], pAGE210 and the like. Any promoter can be used as long as it can be expressed in animal cells.
- CMV cytomegalovirus
- SV40 early promoter examples thereof include a cytomegalovirus (CMV) IE (immediate early) gene promoter, an SV40 early promoter, and a retro-inoles promoter.
- CMV cytomegalovirus
- Examples include the meta mouth thionine promoter, the heat shock promoter, and the SRa promoter.
- the enhancer of the IE gene of human CMV may be used together with the promoter.
- Examples of the host cells include Naraalwa cells, which are human cells, COS cells, which are monkey cells, CH0 cells, which are Chinese hamster cells, and HBT5637. 63-299), rat myeloma cells, mouse myeloma cells, Syrian hamsters, kidney-derived cells, embryonic stem cells, fertilized egg cells, and the like.
- any method for introducing DNA into animal cells can be used.
- electroporation [Cytotechnology, 3, 133 (1990)]
- Calcium phosphate method [Japanese Unexamined Patent Publication (Kokai) No. 2-227075]
- Ribobox method [Proceedings, Op. ]
- Injection method [Manipulating the Mouse Embryo A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press (1994) (hereinafter abbreviated as "Manipi Euling 'Mouse' Emprio 2nd Edition)"
- Particle gun (Genetic gun) a method of using a [Japanese Patent No. 26068 56, Japan Patent No.
- the recombinant virus can be obtained in the insect cell culture supernatant, and further, the recombinant virus can be transmitted to insect cells to express the protein.
- Examples of the gene transfer vector used in the method include pVL1392, pVL1393, pBlueBacIII (all manufactured by Invitorogen) and the like.
- Examples of the paculovirus include the ability to use Autographa californica nuclear polynedrosis virus, which is a virus that infects night-moth insects, such as Autographa californica nuclear polynedrosis virus.
- Autographa californica nuclear polynedrosis virus which is a virus that infects night-moth insects, such as Autographa californica nuclear polynedrosis virus.
- insect cells Sf9 and Sf21, which are ovarian cells of Spodopterafrugiperda [current protocoron's in molecular, bio-biotics, Baculovirus Expression Vectors, A Laboratory Manual, WH Freeman and Company, New York (1992)], Trichoplusia ni Ovarian cells such as High 5 (manufactured by Invitrogen) or the like can be used.
- examples of the expression vector include a Ti plasmid and a tobacco mosaic virus vector.
- Any promoter can be used as long as it can be expressed in plant cells, and examples thereof include the 35S promoter of cauliflower mosaic virus (CaMV) and the geneactin 1 promoter.
- CaMV cauliflower mosaic virus
- host cells include plant cells such as tobacco, potato, tomato, carrot, soybean, oilseed rape, alfa alfa, rice, wheat, and wheat.
- Any method for introducing a recombinant vector can be used as long as it is a method for introducing DNA into plant cells.
- a method using Agrobacterium Japanese Patent Laid-Open No. 59-140885, 60-70080, W094 / 00977
- an electroporation method Japanese Patent Application Laid-Open No. 60-251887
- a method using a particle gun (gene gun) Japanese Patent No. 2517813
- Japanese Patent No. 2517813 Japanese Patent No. 2517813
- a method for expressing an antibody gene in addition to direct expression, secretory production, expression of a fusion protein between the Fc region and another protein, and the like can be performed according to the method described in Molecular Cloning Second Edition. it can.
- an antibody molecule having a sugar or a sugar chain added by the introduced gene may be obtained. it can.
- the antibody composition can be produced by culturing the transformant obtained as described above in a medium, producing and accumulating antibody molecules in the culture, and collecting from the culture.
- the method for culturing the transformant in a medium can be performed according to a usual method used for culturing host cells.
- the medium for culturing the transformant obtained using yeast as a host is a medium containing a carbon source, a nitrogen source, inorganic salts, and the like, which can be used by the organism, and which can efficiently culture the transformant. If so, either a natural medium or a synthetic medium may be used.
- the carbon source may be any one that can be assimilated by the organism; glucose, fructose, sucrose, molasses containing these, carbohydrates such as starch or starch hydrolysate, organic acids such as acetic acid and propionic acid, Alcohols such as ethanol and propanol can be used.
- Nitrogen sources include ammonia, ammonium chloride, ammonium sulfate, ammonium acetate, ammonium phosphate and other ammonium salts of inorganic or organic acids, other nitrogen-containing compounds, and peptone, meat extract, yeast extract, corn steep liquor. , Casein hydrolyzate, soybean meal, soybean meal hydrolyzate, various fermentation cells and digests thereof can be used.
- potassium potassium phosphate potassium potassium phosphate, magnesium phosphate, magnesium sulfate, sodium chloride, ferrous sulfate, man sulfate, copper sulfate, calcium carbonate and the like can be used.
- the culture is usually performed under aerobic conditions such as shaking culture or deep aeration stirring culture.
- the cultivation temperature is preferably 15 to 40 ° C, and the cultivation time is usually 16 hours to 7 days.
- the pH is maintained at 3.0 to 9.0.
- the pH is adjusted using an inorganic or organic acid, an alkaline solution, urea, calcium carbonate, ammonia, or the like.
- an antibiotic such as ampicillin or tetracycline may be added to the medium during the culture.
- an inducer may be added to the medium, if necessary.
- an inducer may be added to the medium, if necessary.
- isopropyl-1] -D-thiogalatatopyranoside can be used to culture microorganisms transformed with a recombinant vector using the trp promoter.
- indoleacrylic acid or the like may be added to the medium.
- a commonly used RPMI1640 medium [The Journal of the American 'Medium Canole' Aso Shay Chaon ( The Journal of the American Medical Association), 199, 519 (1967)], Eagle's MEM medium [Science, 122, 501 (1952)], Dulbecco's modified MEM medium [Virology, 8, 396 ( 1959)], 199 medium [Proceeding of the Society for the Biological Medicine], 73, 1 (1950)], Whitten medium [Developmental engineering experiments Manyua How to make transgenic mice (Kodansha) Motoya Katsuki (ed., 1987)] or a culture medium obtained by adding fetal bovine serum to these culture media can be used.
- Culture is carried out usually p H6 ⁇ 8, 30 ⁇ 40 ° C, 5 % C0 2 present 1 to 7 days under conditions such as lower. Culture can be carried out for one day to several months using culture methods such as hued patch culture and holofiber culture.
- antibiotics such as kanamycin and penicillin may be added to the medium during the culture.
- TNM-FH medium Pulsen
- Sf-900II SFM medium Life Technologies
- ExCell400 ExCell405
- Grace's Insect Medium a medium for culturing a transformant obtained by using insect cells as host cells.
- the cultivation is usually performed under conditions of pH 6 to 7 and 25 to 30 ° C for 1 to 5 days.
- an antibiotic such as gentamicin may be added to the medium during the culture.
- a transformant obtained using a plant cell as a host cell can be cultured as a cell or after being differentiated into a plant cell or organ.
- a culture medium for culturing the transformant a commonly used Murashige and Starg (MS) medium, a white (White) medium, or a plant hormone such as auxin or cytokinin was added to these mediums.
- MS Murashige and Starg
- White white
- plant hormone such as auxin or cytokinin
- Cultivation is usually performed at pH 5 to 9 and 20 to 40 ° C for 3 to 60 days.
- antibiotics such as kanamycin and hygromycin may be added to the medium during the culture.
- a yeast, animal cell, or plant cell-derived transformant harboring a recombinant vector incorporating a DNA encoding an antibody molecule is cultured according to a conventional culture method to produce and accumulate an antibody composition. Then, the antibody composition can be produced by collecting the antibody composition from the culture.
- Methods for producing the antibody composition include a method of producing the antibody composition in a host cell, a method of secreting the antibody out of the host cell, and a method of producing the antibody composition on the host cell outer membrane.
- the method can be selected by changing the structure.
- the method of Paulson et al. the method of Paulson et al. [Journal of Biological 'Chemistry (J. Biol. Chem.), 264, 17619 ( Proc. Natl. Acad. Sci. USA, 86, 8227 (1989); Gene Development. Develop.), 4, 1288 (1990)]
- the methods described in JP-A-05-336963, JP-A-06-823021, and the like whereby the antibody composition is actively secreted out of host cells. Can be done.
- a DNA encoding an antibody molecule and a DNA encoding a signal peptide suitable for expression of the antibody molecule are introduced into an expression vector, and the expression vector is introduced into host cells.
- the target antibody molecule can be actively secreted out of the host cell.
- the production amount can be increased using a gene amplification system using a dihydrofolate reductase gene or the like.
- an individual animal transgenic non-human animal
- individual plant transgenic plant
- An antibody composition can also be produced using an individual.
- the transformant is an animal or plant individual
- the animal is bred or cultivated according to a conventional method to produce and accumulate the antibody composition, and the antibody composition is collected from the animal or plant individual.
- the antibody composition can be produced.
- Examples of a method for producing an antibody composition using an animal individual include, for example, a known method [American Journal of Clinical Nutrition], 63, 639S (1996); American ⁇ Transfer gene according to American Journal of Clinical Nutrition, 63, 627S (1996); Bio / Technology, 9, 830 (1991)]
- a method for producing a desired antibody composition in an animal created by the above method can be used.
- an antibody composition is produced and accumulated in the animal, and the antibody composition is extracted from the animal.
- an antibody composition can be produced.
- Examples of the place of accumulation in the animal include milk of the animal (JP-A-63-309192), eggs, and the like.
- the promoter used in this case Any one can be used as long as it can be expressed in animals.
- a mammary gland cell-specific promoter such as the casein promoter, the casein promoter, the j8 lactoglobulin promoter, the whey acid protein promoter, and the like are preferably used. Used.
- a transgenic plant into which DNA encoding an antibody molecule has been introduced can be prepared by a known method [tissue culture, 20 (1994); tissue culture, 21 (1995)]. ); Cultivated according to Trends in Biotechnology, 15, 45 (1997)] to produce and accumulate an antibody composition in the plant, and to produce the antibody composition from the plant.
- a method of producing an antibody composition by collecting the antibody can be mentioned.
- An antibody composition produced by a transformant into which a gene encoding an antibody molecule has been introduced is, for example, when the antibody composition is expressed in a lysed state in cells, after completion of the culture, the cells are collected by centrifugation. After suspension in an aqueous buffer, the cells are disrupted using an ultrasonic disrupter, French press, Mentongaulin homogenizer, Dynomill, etc. to obtain a cell-free extract.
- a normal enzyme isolation and purification method that is, a solvent extraction method, a salting out method using ammonium sulfate, a desalting method, a precipitation method using an organic solvent, Getylaminoethyl (DEAE)-Sepharose, DIAI0N HPA-75 (manufactured by Mitsubishi Chemical Corporation), etc.
- the cells When the antibody composition is expressed by forming an insoluble form in the cells, the cells are similarly collected, crushed, and centrifuged to collect the insoluble form of the antibody composition as a precipitate fraction.
- the recovered insoluble form of the antibody composition is solubilized with a protein denaturant. After diluting or dialyzing the lysate to restore the antibody composition to a normal three-dimensional structure, a purified sample of the antibody composition can be obtained by the same isolation and purification method as described above.
- the antibody composition can be collected in the culture supernatant. That is, the culture is treated by a technique such as centrifugation as described above. In this manner, a soluble fraction is obtained, and a purified sample of the antibody composition can be obtained from the soluble fraction by using the same isolation and purification method as described above.
- Examples of the antibody composition thus obtained include an antibody, a fragment of the antibody, a fusion protein having an Fc region of the antibody, and the like.
- a humanized antibody expression vector is an expression vector for animal cells into which genes encoding the H chain and L chain C regions of the human antibody have been incorporated. And can be constructed by cloning the genes encoding CL and CL, respectively.
- the C region of a human antibody can be CH and CL of any human antibody.
- the C region of the IgGl subclass of the H chain of the human antibody hereinafter, referred to as hCyl
- human C region of ⁇ class of L chain of the antibody hereinafter referred to as hC K
- genes encoding CH and CL of the human antibody chromosomal DNA consisting of exons and introns can be used, and cDNA can also be used.
- Any expression vector for animal cells can be used as long as it can integrate and express the gene encoding the C region of the human antibody.
- PAGE107 [Cytotechnology, 3, 133 (1990)]
- pAGE103 [Journal of Pio. Biochemistry (j. Biochem.), 101, 1307 (1987)]
- PHSG274 [Gene, 27, 223 (1984)]
- pKCR Proceedings. Op. The 'National' Academy of Sciences (Proc. Natl. Acad. Sci. USA), 78, 1527 (1981)]
- pSGl ⁇ d2- 4 Site Technology (Cytotechnology), 4, 173 (1990)].
- promoters and enhancers used in animal cell expression vectors include the early promoter and enhancer of SV40 [Journal 'ob' Piochemistry (J. Biochem.), 101, 1307 (1987)], Moroni mouse leukemia. Virus LTR Radiochemical 'And Bio-Official Research' Research 'Communications (Biochem. Biophys. Res. Commun.), 149, 960 ( 1987)], immunoglobulin H chain promoter [cell 3 Le (Cell), 41, 479 ( 1985)] and Enhansa Cell (Cell), 33, 717 ( 1983)] and the like 0
- the humanized antibody expression vector must be of either the type in which the antibody H chain and the L chain are present on various vectors or the type in which the antibody is present on the same vector (hereinafter referred to as tandem type). Tandem type, because of the ease of construction of a humanized antibody expression vector, ease of introduction into animal cells, and balance of expression levels of antibody H and L chains in animal cells.
- the vector for expressing a humanized antibody is more preferable [Jan Immunol's "Immunologica Cannole” Methods (J. Immunol. Methods), 167, 271 (1994)].
- the constructed humanized antibody expression vector can be used for expression of a human-type chimeric antibody or a human-type CDR-transplanted antibody in animal cells.
- CDNAs encoding non-human animal antibodies for example, VH and VL of mouse antibodies, can be obtained as follows.
- MRNA is extracted from hybridoma cells producing the desired mouse antibody, and cDNA is synthesized.
- the synthesized cDNA is cloned into a vector such as phage or plasmid to prepare a cDNA library.
- each recombinant plasmid is isolated.
- the entire nucleotide sequence of VH and VL of the target mouse antibody on the recombinant phage or recombinant plasmid is determined, and the entire amino acid sequence of VH and VL is deduced from the nucleotide sequence.
- any animal can be used as long as it can produce hybridoma cells, such as mice, rats, hamsters, and egrets.
- Methods for preparing total RNA from hybridoma cells include guanidine thiocyanate-cesium trifluoroacetate method [Methods in Enzymol., 154, 3 (1987)], and total RNA from RNA.
- a method for preparing mRNA an oligo (dT) -immobilized cellulose column method [Molecular cloning: a lab laboratories, Molecular Cloning: A Laooratory Manual], Cold Spring Harbor Lab. Press New York, 1989] And the like.
- Hypri-Dorma Fine Examples of kits for preparing mRNA from cells include Fast Track mRNA Isolation Kit (Invitrogen), Quick Prep mRNA Purification Kit (Pharmacia) and the like.
- the cDNA library can be prepared by a conventional method [Molecular Cloning: a Laboratory Tree: A Laboratory Manual], Cold Spring Harbor Lab. Press New York, 1989 Current, protocol: ⁇ -norrez in molecular biology, Current Protocols in Molecular Biology, Supplement 34), or some commercial kits, such as Super Script TM Plasmid System for cDNA Synthesis and Plasmid Methods using Cloning (GIBCO BRL) or ZAP-cDNA Synthesis Kit (Stratagene) can be used.
- any vector can be used as a vector for incorporating a cDNA synthesized as a type II mRNA extracted from hybridoma cells, as long as the vector can incorporate the cDNA.
- ZAP Express [Strategies, 5, 58 (1992)]
- pBluescript II SK (+) [Nucleic Acids Research, 17, 9494 (1989)]
- ⁇ zap II (Stratagene)
- Lgtl0 Lgtl0
- ⁇ gtll [DNA Cloning: A Practical Approach, I, 49 (1985)]
- Lambda BlueMid (Clontech)
- ExCell pT7T3 18U (Pharmacia)
- pcD2 [Molecular 'and' Cellular 'biology (Mol. Cell. Biol.), 3, 280 (1983)]
- pUC18 [Gene, 33, 103 (1985)]
- Etc. are used.
- any Escherichia coli can be used as long as the cDNA library can be introduced, expressed and maintained.
- Methods for selecting cDNA clones encoding VH and VL of non-human animal antibodies from a cDNA library include colony hybridization or plaque hybridization using isotope or fluorescently labeled probes. The method can be selected according to the Yon method [Molecular cloning: a laboratory, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Lab. Press New York, 1989].
- PCR method Molecular cloning: a laboratory 1 ⁇ ⁇ manu / cloning (Molecular Cloning) : A Laboratory Manual), Cold Spring Harbor Lab. Press New York, 1989; VH and VL according to Current Protocols in Molecular Biology; Supplement 1-34]. Can also be prepared.
- the cDNA selected by the above method is cleaved with an appropriate restriction enzyme or the like, and then cloned into a plasmid such as pBluescript SK (-) (manufactured by Stratagene). Sanger) et al., And performed a reaction such as the dideoxy method [Proceedings of the National Academy of Sciences (Proc. Natl. Acad. Sci., USA), 74, 5463 (1977)].
- the base sequence of the cDNA can be determined by analysis using a sequence automatic analyzer, for example, ALF DNA Sequencer (Pharmacia).
- the entire amino acid sequence of VH and VL was deduced from the determined base sequence, and the entire amino acid sequence of VH and VL of a known antibody was determined. [Sequences or Proteins of Proteins] Immunological Interest), US Dept. Health and Human Services, 1991] to confirm that the cDNA obtained encodes the complete amino acid sequence of VH and VL of the antibody, including the secretory signal sequence. it can.
- the length of the secretory signal sequence and the N-terminal amino acid sequence can be estimated. You can know which subgroup you belong to.
- the amino acid sequences of the CDRs of VH and VL are also the amino acid sequences of VH and VL of known antibodies [Sequences of proteins, proteins, immunology, Interests, Sequences of Proteins of Immunological Interest, US Dept. Health and Human Services, 1991].
- CDNA encoding VH and VL of non-human animal antibodies is cloned upstream of the genes encoding CH and CL of the human antibodies in the human antibody expression vector described in (1) of Section 3 of this section.
- a human chimeric antibody expression vector can be constructed.
- the cDNA encoding the VH and VL of the antibody of a non-human animal can be obtained by combining the nucleotide sequence at the 3 'end of the VH and VL of the antibody of a non-human animal with the 5- and 5-terminals of CH and CL of the human antibody.
- each of the vectors is a vector for humanized antibody expression as described in (1) of this section 3
- a human chimeric antibody expression vector can be constructed by cloning upstream of the genes encoding CH and CL of the human antibody so that they are expressed in an appropriate form.
- CDNA encoding VH and VL of the human CDR-grafted antibody can be constructed as follows. First, the amino acid sequence of the VH and VL frameworks (hereinafter referred to as FR) of the human antibody to which the VH and VL CDRs of the antibody of the animal other than the target human are transplanted is selected. As the amino acid sequence of FRs of VH and VL of a human antibody, any amino acid sequence derived from a human antibody can be used.
- FR amino acid sequences of human antibody VH and VL registered in databases such as the Protein Data Bank, and common amino acid sequences of FR subgroups of human antibody VH and VL [Sequences''Proteins' of biological interests (Sequences of Proteins of Immunological Interest), US Dept. Health and Human Services, 1991], among which human CDR transplants with sufficient activity
- the amino acid sequences of the CDRs of the VH and VL of the antibody of the non-human animal of interest are transplanted into the amino acid sequences of the FRs of the VH and VL of the selected human antibody, and the VH and VL of the human-type CDR-grafted antibody are grafted.
- Design the amino acid sequence Frequency of codons found in the nucleotide sequence of the designed amino acid sequence in the antibody gene (Sequencys of Proteins of Immunological Interest), US Dept. Health and Human Services, 1991], and design a DNA sequence encoding the amino acid sequence of VH and VL of the human CDR-grafted antibody.
- the amplified product is cloned into a plasmid such as pBluescript SK (-) (Stratagene), the nucleotide sequence is determined by the method described in (2) of this section 3, and the desired human CDR-grafted antibody is obtained.
- a plasmid having a DNA sequence encoding the amino acid sequence of VH and VL is obtained.
- a human CDR-grafted antibody has the antigen-binding activity of the original non-human animal by simply grafting only the VH and VL CDRs of the target non-human animal antibody to the human antibody VH and VL FR. It is known to be lower than antibodies [Bio / Technology (BI0 / TECHN0L0GY), 9, 266 (1991)]. This is due to the fact that not only CDRs but also some amino acid residues of FRs directly or indirectly increase the antigen binding activity in the H chain and L chain V regions of antibodies of animals other than the original human. It is considered that these amino acid residues are changed to different amino acid residues of FR of VH and VL of human antibody upon CDR transplantation.
- human CDR-grafted antibodies have amino acid residues involved in direct binding to antigens and amino acid residues of CDRs in the amino acid sequence of FRs of VH and VL of human antibodies. Identify amino acid residues that interact with groups or maintain the antibody's tertiary structure, and indirectly participate in antigen binding, and identify those amino acid residues found in the original non-human animal antibody Modified to Radio / technologies where increased antigen binding activity is being performed
- a human CDR-grafted antibody In producing a human CDR-grafted antibody, it is of utmost importance how to efficiently identify the amino acid residues of FR involved in the binding activity between the antibody and the antigen.
- Methods for identifying FR amino acids involved in the binding activity between an antibody and an antigen include X-ray crystallography [Journal 'ob' Molecular 'Biology (J. Mol. Biol.), 112, 535 (1977)]
- a method of constructing and analyzing a three-dimensional structure of an antibody such as computer modeling [Protein Engineering, 7, 1501 (1994)] may be used. Information on the three-dimensional structure of these antibodies has provided much useful information for the production of human CDR-grafted antibodies.
- a method for producing a human CDR-grafted antibody applicable to any antibody has not yet been established. Therefore, at present, various trials and errors are necessary, such as preparing several variants of each antibody and examining the correlation with each antigen-binding activity.
- Amino acid residues in FRs of VH and VL of a human antibody can be modified by the PCR method described in 3 (5) of this section using synthetic DNA for modification.
- the nucleotide sequence of the amplified product after PCR is determined by the method described in (2) of this section 3 to confirm that the target modification has been performed.
- the humanized CDR transplant constructed in (5) and (6) of this section 3 was upstream of the genes encoding CH and CL of the human antibody in the humanized antibody expression vector described in (1) of this section 3.
- the cDNA encoding the VH and VL of the antibody can be cloned to construct a human CDR-grafted antibody expression vector.
- an appropriate restriction enzyme at the 5 'end of the synthetic DNA located at both ends By introducing the recognition sequence, the humanized antibody expression vector described in Section 3 (1) is cloned so that it can be expressed in an appropriate form upstream of the genes encoding CH and CL of the human antibody.
- a human CDR-grafted antibody expression vector can be constructed.
- a human chimeric antibody and a human CDR-grafted antibody (hereinafter, also referred to as a humanized antibody) ) Can be obtained stably.
- Examples of a method for introducing a humanized antibody expression vector into animal cells include an electroporation method [Japanese Patent Laid-Open No. 2-257891; Cytotechnology, 3, 133 (1990)] and the like.
- any animal cell that can produce a humanized antibody can be used.
- B HK cells and Namalva cells which are human myeloma cells, and the like, preferably, CH0 / DG44 cells, which are Chinese hamster ovary cells, rat myeloma YB2 / 0 cells, and the cells described in 1. can give.
- G418 sulfate (hereinafter, referred to as G418; SIGMA) according to the method disclosed in JP-A-2-257891. ) Can be selected depending on the animal cell culture medium containing the drug.
- RPMI1640 medium manufactured by Japan-made Kusurisha
- GIT medium EX- CELL 3 02 medium
- JRH Co., Ltd. IMDM medium
- GBCO BRL Hybridoma-SFM medium
- FBS fetal bovine serum
- the production amount and antigen-binding activity of the humanized antibody in the culture supernatant can be measured by an enzyme immunoassay (hereinafter referred to as ELISA method; Antibodies, 'Chapter 14, Monoclonal Antibodies).
- ELISA method enzyme immunoassay
- the transformant can increase the production of humanized antibody using a DHFR gene amplification system or the like according to the method disclosed in Japanese Patent Publication No. 2-257891.
- Humanized antibodies can be purified from the culture supernatant of the transformant using a protein A column [Antipodiz Chapter 8, Monoclonal 'Antibodies].
- other purification methods usually used for protein purification can be used.
- gel filtration, ion exchange mouth chromatography and ultrafiltration Purification can be performed by a combination of excess and the like.
- the molecular weight of the purified humanized antibody H chain, L chain or whole antibody molecule can be determined by polyacrylamide gel electrophoresis [hereinafter referred to as SDS-PAGE; Nature, 227, 680 (1970)] or Western blot. It can be measured by the lotting method [Antibodies: A Laboratory Manual, Chapter 12, Monoclonal Antibody].
- An expression vector for an Fc fusion protein is an expression vector for animal cells into which a gene encoding a protein to be fused with the Fc region of a human antibody is incorporated, and a protein to be fused to the expression vector for animal cells with the Fc region of a human antibody. It can be constructed by cloning the gene encoding
- the Fc region of a human antibody includes a region containing the CH2 and CH3 regions, as well as a region containing the hinge region and a part of CH1. Any compound may be used as long as at least one amino acid of CH2 or CH3 has been deleted, substituted, added or inserted, and has substantially the binding activity to the Fey receptor.
- chromosomal DNA consisting of exons and introns
- cDNA can also be used.
- PCR method Recura-I 'cloning 2nd edition; current' Protocols 'in' Molecular Biology, Supplement 1-34
- Any expression vector for animal cells can be used as long as it can integrate and express a gene encoding the C region of a human antibody.
- PAGE107 Cytotechnology, 3, 133 (1990)
- pAGE103 Journal of Biochem., J.
- promoters and enhancers used in animal cell expression vectors include the early promoter and enhancer of SV40 [Journal 'ob' Piochemistry (J. Biochem.), 101, 1307 (1987)], Moro-I mouse leukemia virus. LTR radiochemical 'and-by' "Research” Communications (Biochem. Biophys. Res. Commun.), 149, 960 (1987)], Immunoglobulin heavy chain promoter [Cell, 41, 479 (1985)] and Enhancer [Cell] (Cell), 33, 717 (1983)].
- DNA encoding a protein to be fused with the Fc region of a human antibody can be obtained as follows.
- MRNA is extracted from cells or tissues that express the protein to be fused with the desired Fc, and cDNA is synthesized.
- the synthesized cDNA is cloned into a vector such as a phage or plasmid to prepare a cDNA library.
- a recombinant phage or a recombinant plasmid having a cDNA encoding the target protein is isolated using the gene sequence portion of the target protein as a probe.
- the entire nucleotide sequence of the target protein on the recombinant phage or recombinant plasmid is determined, and the entire amino acid sequence is estimated from the nucleotide sequence.
- any animals such as mice, rats, hamsters, and egrets, can be used as long as cells and tissues can be removed therefrom.
- Methods for preparing total RNA from cells and tissues include the guanidine thiocyanate-cesium trifluoroacetate method [Methods in Enzymol., 154, 3 (1987)], and mRNA from total RNA.
- Examples of the method for preparing E. coli include the oligo (dT) immobilized lignocellulose column method (Molecular 'Cloning 2nd Edition) and the like.
- Examples of kits for preparing mRNA from cells or tissues include Fast Track mRNA Isolation Kit (Invitrogen), Quick Prep mRNA Purification Kit (Pharraacia) and the like.
- a conventional method (Molecular 'Clothing Second Edition; Current' Protocols', 'Molecular' Biology, Supplement 1-34), or a commercially available kit
- a method using a Super Script TM Plasmid System for cDNA Synthesis and Plasmid Cloning (GIBCO BRL) or a ZAP-cDNA Synthesis Kit (Stratagene) can be used.
- any vector can be used as a vector for incorporating a cDNA synthesized as a type II mRNA extracted from cells or tissues, as long as the vector can incorporate the cDNA.
- ZAP Express [Strategy (Strategies), 5, 58 (1992)], pBluescript II SK (+) [Nucleic Acids Research, 17, 9494 (1989)], ⁇ zapll (Stratagene), L gtlO L gtll [DNA Cloning: A Practical Approach], I, 49 (1985)], Lambda BlueMid (Clontech), L ExCell, pT7T3 18U (Pharmacia) And cD2 [Molecular 'and' Cellular 'biology (Mol. Cell. Biol.), 3, 280 (1983)], pUC18 [Gene, 33.103 (1985)] and the like.
- any Escherichia coli can be used as long as the cDNA library can be introduced, expressed and maintained.
- XLl-Blue MRF [Strategies, 5, 81 (1992)], C600 [Genetics, 39, 440 (1954)], Y1088, Y1090 [Science, 222, 778] (1983)], Marauder 522 [Journal of Molecular Biology (J. Mol. Biol.), 166, 1 (1983)], K802 [Journal of Molecular Biology (J. Mol. Biol.)] Biol.), 16, 118 (1966)] and JM105 [Gene, 38, 275 (1985)].
- Methods for selecting cDNA clones encoding the desired protein from the cDNA library include colony hybridization using an isotope or fluorescently labeled probe, or plaque 'hybridization' (Molecularization Cloning). 2 edition).
- primers can be prepared, and cDNA encoding the target protein can be prepared by PCR using a cDNA or a cDNA library synthesized from raRNA as a type II.
- a PCR method can be used as a method for fusing the target protein with the Fc region of a human antibody. For example, an arbitrary synthetic oligo DNA (primer) is set on the 5 ′ side and 3 ′ side of the gene sequence of the target protein, and a PCR method is performed to obtain a PCR product. Similarly, an arbitrary primer is set for the gene sequence of the Fc region of the human antibody to be fused, and a PCR product is obtained. At this time, primers are set so that the same restriction enzyme site or the same gene sequence exists on the 3 ′ side of the PCR product of the protein to be fused and the 5 ′ side of the PCR product of the Fc region.
- primers are set so that the same restriction enzyme site or the same gene sequence exists on the 3 ′ side of the PCR product of the protein to be fused and the 5 ′ side of the PCR product of the Fc region.
- the mutation is introduced by using a primer into which the mutation has been introduced.
- the resulting genes are further subjected to PCR using the two types of PCR fragments to ligate both genes.
- ligation can be performed by performing ligation after the same restriction enzyme treatment.
- the gene sequence ligated by the above method is cut with an appropriate restriction enzyme or the like, and then cloned into a plasmid such as pBluescript SK (-) (manufactured by Stratagene), and a commonly used nucleotide sequence analysis method, for example, Sanger The dideoxy method [Proceedings of Ob. 'The' National 'Academy' Ob '' Science (Proc. Natl. Acad. Sci. USA), 74, 5463 (1977)] or ABI PRISM 377 DNA Theta-Encer (PE
- the base sequence of the DNA can be determined by analysis using a base sequence analyzer such as Biosystems.
- a transformant capable of stably producing the Fc fusion protein can be obtained.
- Examples of a method for introducing the Fc fusion protein expression vector into animal cells include the electoporation method [Japanese Patent Laid-Open No. 2-257891; Cytotechnology, 3, 133 (1990)] and the like.
- any animal cell that can produce the Fc fusion protein can be used. Specifically, it is derived from mouse myeloma cells NS0 cells, SP2 / 0 cells, Chinese hamster ovary cells CHO / dhfr- cells, CH0 / DG44 cells, rat myeloma YB2 / 0 cells, IR983F cells, and Syrian hamster kidney.
- Examples include BHK cells, Namalva cells which are human myeloma cells, etc., preferably, CH0 / DG44 cells which are Chinese hamster monoovary cells, rat myeloma YB2 / 0 cells, and the method of the present invention according to the above item 1.
- the host cell to be used is exemplified.
- a transformant that stably produces the FcS insect mating protein is selected using an animal cell culture medium containing a drug such as G418 according to the method disclosed in Japanese Patent Application Laid-Open No. 2-257891. it can.
- culture media for animal cells RPMI1640 medium (Nissui Pharmaceutical), GIT medium (Nippon Pharmaceutical), EX-CELL302 medium (JRH), IMDM medium (GIBCO BRL), Hybridoma-SFM medium (Manufactured by GIBCO BRL) or a medium obtained by adding various additives such as fetal bovine serum to these media.
- the Fc fusion protein is produced and accumulated in the culture supernatant. Can be made.
- the production amount and antigen-binding activity of the Fc fusion protein in the culture supernatant can be measured by ELISA or the like.
- the transformed strain can increase the production amount of the Fc fusion protein using a dhfr gene amplification system or the like according to the method disclosed in JP-A-2-2577891.
- the Fc fusion protein can be purified from the culture supernatant of the transformant using a protein A column or a protein G column (Antibodies, Chapter 8, Monoclonal Antibody).
- a protein A column or a protein G column Antibodies, Chapter 8, Monoclonal Antibody
- other purification methods usually used for protein purification can be used.
- purification can be performed by a combination of gel filtration, ion exchange chromatography, ultrafiltration, and the like.
- the molecular weight of the whole purified Fc fusion protein molecule is measured by SDS-PAGE [Nature, 227, 680 (1970)], Western blotting (Antibodies, Chapter 12, Monoclonal 'Antibodies'), etc. be able to.
- the method for producing an antibody using an animal cell as a host has been described above. However, as described above, the antibody can be produced in an enzyme, an insect cell, a plant cell, an animal individual, or a plant individual.
- antibody-producing cells are prepared using the method described in 1 above, and then the cells are cultured.
- the antibody composition can be produced by purifying the antibody composition
- the binding activity to an antigen and the binding activity to an antigen-positive cultured cell line can be determined by ELISA and immunofluorescence [Cancer Immunology] Immunotherapy. (Cancer Immunol. Iramunother.), 36, 373
- Cytotoxic activity against an antigen-positive cultured cell line can be evaluated by measuring complement-dependent cytotoxicity (hereinafter, referred to as CDC activity), ADCC activity, etc. [Cancer's Immunology / Immunotherapy]
- the sugar chain structure of the antibody molecule expressed in various cells can be determined according to the analysis of the sugar chain structure of a normal glycoprotein.
- the sugar chains bound to IgG molecules are composed of neutral sugars such as galactose, mannose, and fucose, amino sugars such as N-acetyldarcosamine, and acidic sugars such as sialic acid.
- the analysis can be performed using techniques such as composition analysis and sugar chain structure analysis using a two-dimensional sugar chain mapping method.
- composition analysis of the sugar chain of an antibody molecule neutral sugar or amino sugar is released by acid hydrolysis of the sugar chain with trifluoroacetic acid or the like, and the composition ratio can be analyzed.
- BioLC sugar composition analyzer
- HPAEC-PAD high performance anion-exchange chromatography—pulsed amperometric detection
- the composition ratio can also be analyzed by a fluorescent labeling method using 2-aminopyridine. Specifically, a sample obtained by acid hydrolysis according to a known method [Agrucural and Biological Chemistry (Agruc. Biol. Chem.), 55 (1), 283-284 (1991)] is used. -Fluorescent labeling by aminopyridylation and HPLC analysis can calculate the composition ratio.
- Structural analysis of the sugar chain of the antibody molecule is performed by two-dimensional sugar chain mapping [Analytical Biochemistry., 171, 73 (1988), Biochemistry Experimental Method 23—Glycoprotein sugar chain research method (Academic Society) Publishing Center) Edited by Reiko Takahashi (1989)].
- the X-axis shows the retention time or elution position of the reversed-phase chromatography sugar chain
- the Y-axis shows the retention time of the sugar chain by normal-phase chromatography. This method estimates the sugar chain structure by plotting W or the elution position, and comparing them with the results of known sugar chains.
- the antibody is hydrazinolyzed to release a sugar chain from the antibody, and fluorescent labeling of the bran chain with 2-aminopyridine (hereinafter abbreviated as PA) [Journal Oppy Biochemistry (J. Biochem.), 95, 197 (1984)], and the sugar chains are separated from excess PA reagent by gel filtration, followed by reverse phase chromatography. Next, normal phase chromatography is performed on each peak of the separated sugar chain. Based on these results, the results were plotted on a two-dimensional glycan map and compared with the glycan standard (TaKaRa) and the literature [Analytical 'Biochemistry., 171, 73 (1988)]. The sugar chain structure can be estimated from the comparison of the spots.
- PA 2-aminopyridine
- mass spectrometry such as MALDI-T0F-MS of each sugar chain can be performed to confirm the structure estimated by the two-dimensional sugar chain mapping method.
- the antibody composition obtained according to the present invention has high ADCC activity.
- Antibodies having high ADCC activity are useful in the prevention and treatment of various diseases including cancer, inflammatory diseases, autoimmune diseases, immune diseases such as allergies, cardiovascular diseases, and viral or bacterial infections.
- Cancer cells ie, malignant tumors, have proliferating cancer cells.
- Ordinary anticancer drugs are characterized by inhibiting the growth of cancer cells.
- an antibody having high ADCC activity can treat cancer by damaging cancer cells by a cell-killing effect, and is therefore more effective as a therapeutic agent than ordinary anticancer drugs.
- the antitumor effect of antibody drugs alone is often insufficient at present, and combination therapy with chemotherapy is performed [Science, 280, 1197, 1998]. If a stronger antitumor effect of the antibody composition alone obtained by is obtained, the dependence on chemotherapy is reduced, which leads to a reduction in side effects.
- the in vivo reaction in those diseases is triggered by the release of mediator molecules by the immune cells, so the immune cells are eliminated using antibodies with high ADCC activity Thereby, the energy reaction can be suppressed.
- Cardiovascular diseases include arteriosclerosis. Atherosclerosis is currently treated with balloon catheters, but ADCC increases arterial cell proliferation in restenosis after treatment. Suppression with an active antibody can prevent and treat circulatory diseases.
- Antibodies that recognize tumor-associated antigens, recognize allergy or inflammation-related antigens, recognize circulatory disease-related antigens, recognize autoimmune disease-related antigens, or respond to viral or bacterial infections Specific examples of antibodies that recognize related antigens are described below.
- Antibodies that recognize tumor-associated antigens include anti-GD2 antibody (Anticancer Res., 13, 331-336, 1993), anti-GD3 antibody (Cancer Immunol. Imraunother., 36, 260-266, 1993), anti-GM2 antibody (Cancer Res., 54, 1511-1516, 1994), anti-HER2 antibody
- Anti-basic fibroblast growth factor antibody Anti-basic fibroblast growth factor antibody, anti-FGF8 receptor antibody (J. Biol. Chem., 265, 16455-16463, 1990) , Anti-basic fibroblast growth factor receptor antibody, anti-insulin-like growth factor antibody (J. Neurosci. Res., 40, 647-659, 1995), anti-insulin-like growth factor receptor antibody (J. Neurosci. Res. ., 40, 647-659, 1995), anti-PMSA antibody (J.
- Antibodies that recognize antigens associated with allergy or inflammation include anti-interleukin-6 antibody (Immunol. Rev., 127, 5-24, 1992) and anti-interleukin-6 receptor antibody (Molecular Immunol., 31, 371). -381, 1994), anti-interleukin 5 antibody (Immunol. Rev., 127, 5-24, 1992), anti-interleukin 5 receptor antibody, Interleukin-4 antibody (Cytokine, 3, 562-567, 1991), anti-interleukin-4 receptor antibody (J. Immunol.
- anti-tumor necrosis factor antibody Hybridoma, 13, 183-190, 1994
- anti-tumor necrosis factor receptor antibody Molecular Pharmacol., 58, 237-245, 2000
- anti-CCR4 antibody Nature, 400, 776-780
- anti-chemokine antibody J. Immunol. Meth., 174, 249-257, 1994
- anti-chemoforce receptor antibody J. Exp. Med., 186, 1373-1381, 1997)
- anti-IgE antibody Anti-CD23 antibody
- anti-CDlla antibody Immunology Today, 21 (8), 403-410 (2000)
- anti-CRTH2 antibody J Immunol., 162, 1278-1286 (1999)
- anti-CCR8 antibody W099 / 25734
- Anti-CCR3 antibody US6207155
- Antibodies that recognize antigens associated with cardiovascular diseases include anti-GpIIb / IIIa antibodies (J. Immunol., 152, 2968-2976, 1994) and anti-platelet-derived growth factor antibodies (Science, 253, 1129-1132, 1991). ), Anti-platelet-derived growth factor receptor antibody (J. Biol. Chem., 272, 17400-17404, 1997) or anti-coagulation factor antibody (Circulation, 101, 1158-1164,
- Antibodies that recognize antigens associated with autoimmune diseases include anti-self DNA antibodies
- Antibodies that recognize antigens associated with viral or bacterial infection include anti-gp120 antibody (Structure, 8, 385-395, 2000), anti-CD4 antibody (J. Rheumatology, 25, 2065-2076, 1998), anti-CCR4 Antibodies, anti-verotoxin antibodies (J. Clin.
- autoimmune diseases psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, systemic lupus erythematosus, multiple sclerosis, etc.
- Anti-CDlla antibody, anti-ICAM3 antibody, anti-CD80 antibody, anti-CD2 antibody, anti-CD3 antibody, anti-CD4 antibody, anti-integrin ⁇ 4] 37 antibody, anti-CD40L antibody, anti-IL-2 receptor antibody Immunology Today, 21 (8), 403-410
- the above antibodies were obtained from public institutions such as ATCC (The American Type Culture Collection), RIKEN Cell Development Bank, Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology, or Dainippon Pharmaceutical Co., Ltd., R & D SYSTEMS, PharMingen, It can be obtained from commercial reagent sales companies such as Cosmo Bio Inc. and Funakoshi Inc.
- the medicament containing the antibody composition obtained by the present invention can be administered alone as a therapeutic agent, it is usually mixed with one or more pharmacologically acceptable carriers, and It is desirable to provide it as a pharmaceutical preparation produced by any method well known in the pharmaceutical art. It is desirable to use the most effective route for treatment, including oral administration or parenteral administration such as buccal, respiratory, rectal, subcutaneous, intramuscular and intravenous administration. In the case of an antibody preparation, intravenous administration is preferred.
- Dosage forms include sprays, capsules, tablets, granules, syrups, emulsions, suppositories, injections, ointments, tapes and the like.
- Formulations suitable for oral administration include emulsions, syrups, capsules, tablets, powders, granules and the like.
- Liquid preparations such as emulsions and syrups include water, sugars such as sucrose, sorbitol, fructose, glycols such as polyethylene glycol and propylene glycol, oils such as sesame oil, olive oil, soybean oil, p-hydroxy It can be produced using preservatives such as benzoic acid esters and flavors such as strawberry flavor and peppermint as additives.
- Capsules, tablets, powders, granules, etc. are excipients such as lactose, pudose, sucrose, manitol, disintegrants such as starch and sodium alginate, lubricants such as magnesium stearate, talc, poly It can be produced using a binder such as BURU alcohol, hydroxypropyl cellulose or gelatin, a surfactant such as fatty acid ester, or a plasticizer such as glycerin as an additive.
- a binder such as BURU alcohol, hydroxypropyl cellulose or gelatin, a surfactant such as fatty acid ester, or a plasticizer such as glycerin as an additive.
- Formulations suitable for parenteral administration include injections, suppositories, sprays and the like.
- An injection is prepared using a carrier comprising a salt solution, a glucose solution, or a mixture of both.
- a powder injection can be prepared by freeze-drying the antibody composition according to a conventional method and adding sodium chloride thereto.
- Suppositories are prepared using carriers such as rosin, hydrogenated fats or carboxylic acids.
- Sprays are prepared using the antibody composition itself or a carrier that does not irritate the oral and respiratory tract mucosa of the recipient and disperses the antibody composition as fine particles to facilitate absorption.
- the carrier include lactose and glycerin.
- Formulations such as aerosols and dry powders are possible depending on the properties of the antibody composition and the carrier used. Also, in these parenteral preparations, the components exemplified as additives in the oral preparation can be added.
- the dose or frequency of administration varies depending on the desired therapeutic effect, administration method, treatment period, age, body weight, etc., but is usually 10 / ig / kg to 20 mg / kg per adult per day.
- Methods for examining the antitumor effect of the antibody composition on various tumor cells include CDC activity measurement and ADCC activity measurement in in vitro experiments, and in vivo experiments on mice and other experimental animals. Antitumor experiments using such tumor systems.
- FIG. 1 shows the ADCC activity of the anti-CCR4 chimeric antibody produced by rat myeloma YB2 / 0 cell KM2760 # 58-35-16 strain 1-15 strain against CCR4 / EL-4 cells. .
- the vertical axis indicates cytotoxic activity, and the horizontal axis indicates antibody concentration.
- the mouth indicates the activity of KM2760 # 58-35-16 produced anti-CCR4 chimeric antibody KM2760-1, and the open triangle indicates the activity of anti-CCR4 chimeric antibody 1-15 produced KM2760-2.
- FIG. 2 shows elution diagrams obtained by reverse-phase HPLC analysis of PA-glycans prepared from anti-CCR4 chimeric antibodies produced by mfFUT8-6 and pAGE249-introduced strains.
- FIG. 2A shows the elution diagram of the PA-glycan prepared from the antibody produced by the mfFUT8-6 strain
- FIG. 2B shows the elution diagram of the PA-glycan prepared from the antibody produced by the PAGE249-transformed strain.
- the vertical axis shows the relative fluorescence intensity
- the horizontal axis shows the elution time.
- FIG. 3 is a photograph showing the results of quantification of the amounts of FUT8 and actin transcripts in each host cell strain using the RT-PCR method.
- CDNA prepared from ⁇ 2760-1 producing strain ⁇ 2760 # 58-35-16, ⁇ 2760-2 producing strain 1-15, and parental rat myeloma ⁇ 2 / 0 cells was designated as ⁇ type and FU8 specific.
- the results obtained by performing a PCR reaction using a primer set (SEQ ID NOS: 13 and 14) or a] 3-actin-specific primer (SEQ ID NOs: 11 and 12) and subjecting the reaction solution to agarose gel electrophoresis are shown.
- FIG. 4 is a diagram showing the construction of plasmid ploxPPuro.
- FIG. 5 is a diagram showing the construction of a plasmid P K0FUT8gE2- 1.
- FIG. 6 is a diagram showing the construction of plasmid pK0FUT8gE2-2.
- FIG. 7 shows the construction of plasmid pscFUT8gE2-3.
- FIG. 8 is a diagram showing the construction of plasmid pK0FUT8gE2-3.
- FIG. 9 is a diagram showing the construction of plasmid pK0FUT8gE2-4.
- FIG. 10 is a diagram showing the construction of plasmid pK0FUT8gE2_5.
- FIG. 11 is a diagram showing the construction of plasmid pK0FUT8Puro.
- Fig. 12 shows the broken 1,0-fucosyltransferase gene CH0 cell line.
- 1 is a photograph showing the results of genomic Southern analysis of 1st. AFUT8 2-46-1 strain and 1st. AFUT8 2-46 strain.
- FIG. 13 shows the results of genomic Southern analysis of the ⁇ 1,6-fucosyltransferase (FUT8) gene disrupted CH0 cell line, lst. AFUT8 2-46 strain and 2-46-H10 strain.
- FIG. 14 is a photograph showing ADCC activity of the anti-CCR4 chimeric antibody purified from the FUT8 allele-disrupted strain.
- the vertical axis indicates cytotoxic activity, and the horizontal axis indicates antibody concentration.
- ⁇ and Awake indicate the activity of the purified antibody derived from the anti-CCR4 chimeric antibody-producing CH0 cell 5-03 strain and the purified antibody derived from the 1st. AFUT8 2-46-1 strain, respectively.
- FIG. 15 is a diagram showing the construction of a plasmid P K0FUT8Neo.
- FIG. 16 is a photograph showing the result of genomic southern analysis of a clone in which one copy of the FUT8 allele of CH0 / DG44 cells was disrupted.
- FIG. 17 is a photograph showing the result of genomic southern analysis of a clone in which both FUT8 alleles of CH0 / DG44 cells were disrupted.
- FIG. 18 is a photograph showing the results of genomic Southern analysis of a clone in which drug resistance genes have been removed from both FUT8 alleles of CH0 / DG44 cells.
- FIG. 19 is a diagram showing the construction of plasmid pBs-2B8L.
- FIG. 20 is a diagram showing the construction of plasmid pBs-2B8H and plasmid pBs_2B8Hm.
- FIG. 21 is a diagram showing the construction of plasmid pKANTEX2B8P.
- FIG. 22 is a view showing ADCC activity of an anti-CD20 chimeric antibody purified from a CH0 / DG44 cell-derived FUT8 gene double knockout strain on a human B lymphocyte culture cell line Raji.
- the vertical axis indicates cytotoxic activity, and the horizontal axis indicates antibody concentration.
- FIG. 23 is a diagram showing the construction of plasmid CHfFUT8-pCR2.1. BEST MODE FOR CARRYING OUT THE INVENTION
- Example 1 Preparation of cells stably producing anti-CCR4 chimeric antibody
- MTX (SIGMA), which is an agent, is suspended in Hybridoma-SFM-FBS (5) medium containing ⁇ to a concentration of 1-2 ⁇ 10 5 cells / ml, and placed on a 24 ⁇ : plate (Greiner). Each 1 ml was dispensed. After culturing at 37 ° C. for 1 to 2 weeks in a 5% CO 2 incubator, a transformant showing 50 nM MTX resistance was induced. The antigen-binding activity of the anti-CCR4 chimeric antibody in the culture supernatant of the wells in which the growth of the transformed strain was observed was measured by the ELISA method described in Example 2 (2).
- IMDM- dFBS (10) medium was changed to (dialyzed FBS IMDM medium containing 10%), were cultured for 1-2 weeks. Since a colony of a transformant showing HT-independent growth appeared, the culture supernatant was recovered from the wells in which growth was observed, and the expression level of the anti-CCR4 chimeric antibody in the supernatant was determined in Example 2 (2). It was measured by the ELISA method described.
- IMDM-dFBS 10 nM containing 50 nM MTX
- the cells were suspended in a medium at a concentration of 1-2 ⁇ 10 5 cells / ml, and 0.5 ml was dispensed into 24 ⁇ l plates (manufactured by Iwaki Glass). After culturing at 37 ° C. for 1 to 2 weeks in a 5% CO 2 incubator, a transformant showing 50 nM MTX resistance was induced.
- the MTX concentration was increased to 200 nM by the same method as described above, and finally, it was possible to grow on IMDM-dFBS (10) medium containing MTX at a concentration of 200 nM.
- a transformant highly producing an anti-CCR4 chimeric antibody was obtained.
- the resulting transformant was named 5-03 strain.
- Example 2 Comparison of antibody composition produced by antibody-producing cells with reduced expression of FUT8 gene and antibody composition produced by parental cells
- the ADCC activity was compared between an antibody composition produced by an antibody-producing cell in which the activity of the 1,6-fucose modifying enzyme was lower than that of the parent cell and an antibody composition produced by the parent cell.
- KM2760 # 58-35-16 having a low transcript amount of the FUT8 gene described in (1) of Example 1 is used.
- the antibody composition KM2760-1 purified from the culture supernatant of the strain was used.
- the antibody composition produced by the rat myeloma YB2 / 0 cell (ATCC CRL1662) as the parent cell line was prepared as follows.
- Hybridoma-SFM containing ⁇ at a concentration of 200 ⁇ was finally raised by the same method as above.
- -A transformant capable of growing in the FBS (5) medium and highly producing an anti-CCR4 chimeric antibody was obtained.
- the obtained transformant was designated as 1-15 strain.
- the 1-15 strains were not converted into single cells (cloning) by the limiting dilution method.
- the anti-CCR4 chimeric antibody produced by the obtained transformant strain 1-15 was purified as follows.
- Hybridoma-SFM manufactured by Invitrogen
- a transformant 1-15 expressing the anti-CCR4 chimeric antibody was cultured in 2 ⁇ 10 5 cells / cell.
- the mixture was suspended in a constant-temperature room at 37 ° C using a spinner bottle (manufactured by Iwaki Glass) and stirred and cultured. Culture supernatant collected after culturing for 8-10 days
- the anti-CCR4 chimeric antibody was purified using a Prosep_A (Millipore) column and gel filtration.
- the purified anti-CCR4 chimeric antibody was named KM2760-2.
- the antigen binding activity of the two types of antibody compositions obtained in Example (1) was measured by the following ELISA method.
- Compound 1 (SEQ ID NO: 6) was selected as a human CCR4 extracellular domain peptide to which the anti-CCR4 chimeric antibody can react.
- a conjugate with BSA Bovine Serum Albumin (manufactured by Nacalai Tesque, Inc.) was prepared by the following method and used as an antigen to be used for activity measurement by ELISA. That is, 100 mL of 25 mg / mL SMCC [4- (N-maleimide methyl) cyclohexane-1-carboxylic acid N-hydroxysuccinimide ester] was added to 900 mL of PBS solution containing 10 mg of BSA (manufactured by Sigma).
- the conjugate prepared as above was dispensed into a 96-well EIA plate (manufactured by Grainer Co., Ltd.) at 0.05 Aig / mL, 501 / ml, and left at 4 ° C for adsorption. I let it. After washing with PBS, 1% BSA-PBS was added at lOO / z L / well, and reacted at room temperature for 1 hour to block the remaining active groups. After washing each well with PBS containing 0.05% Tween20 (hereinafter referred to as TweeN-PBS), the culture supernatant of the transformed strain was added at 50 L / well, and reacted at room temperature for 1 hour.
- TweeN-PBS PBS containing 0.05% Tween20
- ADCC activity of the purified KM2760-1 and KM2760-2 was measured by the following method.
- the ADCC activity of the anti-CCR4 chimeric antibody on CCR4 / EL-4 cells was measured as follows.
- the target cell solution prepared in (1) above was dispensed into each well of a 96-well U-bottom plate (Falcon). Then, the human effector cell solution prepared in the above (2) was added in an amount of 100 iL (5 ⁇ 10 5 cells / well, the ratio of one human effecta cell to the target cell was 50: 1). Further, an anti-CCR4 chimeric antibody was added to each final concentration of 0.0001 to 10 ⁇ g / mL, and the mixture was reacted at 37 ° C. for 4 hours. After the reaction, the plate was centrifuged, and the amount of 51 Cr in the supernatant was measured with a counter.
- the amount of spontaneously dissociated 51 Cr was determined by performing the same operation as above using only the medium instead of the human effector cell solution and the antibody solution, and measuring the amount of 51 Cr in the supernatant. All solution The amount of released 51 Cr was determined by adding an lmol / L hydrochloric acid solution instead of the antibody solution and the human effector cell solution, performing the same operation as above, and measuring the amount of 51 Cr in the supernatant. ADCC activity (%) was determined by the following equation (I).
- ADCC activity (%) X 100 (I)
- the sugar chains of KM2760-1 and KM2760-2 were analyzed according to the following method.
- the sugar chain fraction was dried with a centrifugal concentrator to obtain a purified PA-linked sugar chain group.
- reverse-phase HPLC analysis of the purified PA-glycans was performed using a CLC-0DS column (manufactured by Shimadzu) (Fig. 2).
- Buffer B (%) 0 60 60 0 0
- the peaks (1) to (8) shown in FIG. 2 have the following structures, respectively.
- GlcNAc is N-acetyldarcosamine
- Gal is galactose
- Man is mannose
- Fuc is fucose
- PA is a pyridylamino group.
- the ratio of the sugar chains having no eel, 6 -fucose is the area occupied by the peaks (1) to (4) among (1) to (8), and al, 6 -fucose is bound.
- the ratio of the sugar chain group was calculated from the area occupied by the peaks (5) to (8) among (1) to ( 8 ).
- FIG. 3 shows the results of measuring the amount of the substance according to the quantification method described in Example 8 of W000 / 61739.
- the expression level of the FUT8 gene in the 1-15 strain was equivalent to that of the parental cell line, rat myeloma YB2 / 0 cells, but the expression level of the KM2760 # 58-35-16 strain was clearly lower than these strains.
- antibody-producing cells in which the activity of ct1,6-fucose modifying enzyme is lower than that of the parent cell line can produce an antibody composition having higher ADCC activity than the antibody composition produced by the parent cell line. It was confirmed that there was.
- Example 3 Preparation of CH0 cells in which the FUT8 gene has been disrupted and production of antibodies using the cells
- CH0 cells CH0 cells deleted from the genomic region containing etason 2 of the FUT8 gene were prepared, and the ADCC activity of antibodies produced by the cells was evaluated.
- Plasmid ploxPPuro was constructed by the following procedure (Fig. 4).
- the plasmid ploxPl.Owg described in JP-A-11-314512 was dissolved in 35 ⁇ l of NEBuffer 4 (manufactured by New England Biolabs), and 20 units of a restriction enzyme (manufactured by New England Biolabs) was added thereto. The digestion reaction was performed with C for 2 hours. After the digestion reaction,
- Plasmid pFUT8fgE2-2 which has a genomic region containing exon 2 of Chinese hamster FUT8 obtained in Reference Example (2)
- plasmid pK0FUT8gE2-l was constructed according to the following procedure (FIG. 5).
- the enzyme EcoRV (New England Biolabs) was added and digestion reaction was performed at 37 ° C for 2 hours. After the digestion reaction, the solution was subjected to 0.8% (w / v) agarose gel electrophoresis to purify a DNA fragment of about 1.5 Kb.
- a plasmid pK0FUT8gE2-2 was constructed according to the following procedure (FIG. 6).
- the DNA fragment was dissolved in 30 il of NEBuffer 1 (manufactured by New England Biolabs) containing lOO ⁇ ug / ml BSA (manufactured by New England Biolabs), and 20 units of The digestion reaction was performed at 37 ° C for 2 hours with the addition of restriction enzyme Kpnl (New England Biolabs). After the digestion reaction, the solution was subjected to 0.8% (w / v) agarose gel electrophoresis to purify a DNA fragment of about 1.5 Kb.
- the plasmid ploxPPuro 1. is dissolved in 30 ⁇ l of NEBuffer 4 (New England Biolabs), and 20 units of restriction enzyme 1 ⁇ 1 (New England Biolabs) is added. The digestion reaction is performed at 37 ° C for 2 hours. went. A DNA fragment was recovered from the reaction solution by the ethanol precipitation method, and then dissolved in 30 ⁇ l of NEBuffer 1 (New England Biolabs) containing lOO ⁇ g / ml BSA (New England Biolabs). Unit restriction enzyme (New England Biolabs) was added and digestion reaction was performed at 37 ° C for 2 hours. After the digestion reaction, the solution was subjected to 0.8% (w / v) agarose gel electrophoresis to purify a DNA fragment of about 3.5 Kb.
- Plasmid pscFUT8gE2-3 was constructed by the following procedure using plasmid P FUT8fgE2-4 having a genomic region containing exon 2 of Chinese hamster FUT8 obtained in Reference Example (2) (FIG. 7).
- Hpall-Hindlll fragment (about 3.5 Kb) derived from plasmid pFUT8fgE2-4 (about 3.5 Kb) obtained above, EcoRV-Hindlll fragment (about 2.8 Kb) derived from plasmid LITMUS39 (about 2.8 Kb) 1.0 ⁇ l, Ligation High (Toyobo 5.0 ⁇ l were mixed and reacted at 16 ° C. for 30 minutes to perform a binding reaction. The reaction solution was used to transform Escherichia coli DH5a strain, and plasmid DNA was isolated from the obtained ampicillin-resistant clones in accordance with a known method. This plasmid is hereinafter referred to as pscFUT8gE2-3.
- plasmid pBluescriptll KS (+) (Strategene) was dissolved in 3B ⁇ 1 of NEBuffer for EcoRI (New England Biolabs), and 20 units of restriction enzyme EcoRI (New England Biolabs) and The digestion reaction was performed for 2 hours at 37 ° C. by adding 20 units of restriction enzyme (1111 (manufactured by New England Biolabs)). The solution was subjected to 0.8% (w / v) agarose gel electrophoresis to purify a DNA fragment of about 3.0 Kb.
- Hindlll-EcoRI fragment (about 1.8 Kb) derived from plasmid pFUT8fgE2-4 (about 1.8 Kb) 4.0 ⁇ 1, Hindlll-EcoRI fragment (about 3.0 Kb) derived from plasmid pBluescriptll KS (+) 1.0 ⁇ 1, Ligation High (manufactured by Toyobo Co., Ltd.) 5.0 ⁇ 1 was mixed and reacted at 16 ° C for 30 minutes to perform a binding reaction. The reaction solution was used to transform Escherichia coli DH5a strain, and plasmid DNA was isolated from the obtained ampicillin-resistant clones in accordance with a known method. This plasmid following, P K0FUT8gE2 - referred to 3.
- the plasmid pscFUT8gE2-31.0 / ig was dissolved in NEBuffer for Sail (New England Biolabs) 351 containing lOO g / ml BSA (New England Biolabs) and the restriction enzyme il (New England Biolabs)
- the digestion reaction was performed at 37 ° C for 2 hours after adding 20 units.
- the DNA fragment was dissolved in NEBuffer 2 (New England Biolabs) 30/1, and 20 units of restriction enzyme Hindi II (New England Biolabs) was added.
- the digestion reaction was performed at 37 ° C for 2 hours.
- the solution was subjected to 0.8% (w / v) agarose gel electrophoresis to purify a DNA fragment of about 3.6 Kb.
- plasmid pK0FUT8gE2-3 was dissolved in 35 ⁇ l of NEBuffer for Sail (New England Biolabs) containing 100 ⁇ g / ml BSA (New England Biolabs), and the restriction enzyme Sail ( (New England Biolabs) 20 units were added and digestion reaction was performed at 37 ° C for 2 hours. After recovering a DNA fragment from the reaction solution by an ethanol precipitation method, the DNA fragment was dissolved in NEBuffer 2 (New England Biolabs) 35 1, and 20 units of restriction enzyme Hindlll (New England Biolabs) was added. The digestion reaction was performed at ° C for 2 hours.
- the Sail-HindiII fragment (approximately 3.1 Kb) derived from the plasmid pscFUT8gE2-3 (approximately 3.1 Kb) obtained above, the Sail-Hindlll fragment (approximately 4.8 Kb) derived from the plasmid pK0FUT8gE23 1.01, Ligation High (Toyobo 5.0 ⁇ l were mixed and reacted at 16 ° C. for 30 minutes to perform a binding reaction. Escherichia coli DH5 strain was transformed using the reaction solution, and plasmid DNA was isolated from the obtained ampicillin-resistant clones in accordance with a known method. This plasmid is hereinafter referred to as pK0FUT8gE2-4.
- plasmid PK0FUT8gE2- 2 and PK0FUT8gE2- 4 obtained in the item (3) and (6), to construct a plasmid pK0FUT8 g E2- 5 by the following procedure (Fig. 10).
- the plasmid pK0FUT8gE2-41.0 ⁇ g was dissolved in NEBu; fer4 (New England Biolabs) 30 ⁇ 1 and added with 20 units of restriction enzyme (New England Biolabs) for 2 hours at 25 ° C.
- a digestion reaction was performed. After recovering the DNA fragment from the reaction solution by using the ethanol precipitation method, the DNA fragment was dissolved in NEBuffer 2 (manufactured by New England Biolabs) 301, and 20 units of restriction enzyme BamHI (manufactured by New England Biolabs) was added thereto. The digestion reaction was performed at C for 2 hours. After the digestion reaction, the solution was subjected to 0.8% (w / v) agarose gel electrophoresis to purify a DNA fragment of about 5.2 Kb.
- Dissolve plasmid pKOSelectDT (Lexicon) 1.0 / ig in 50 ⁇ NE of NEBuffer 4 (New England Biolabs), add 16 units of restriction enzyme ⁇ ⁇ (New England Biolabs) and add at 37 ° C. The digestion reaction was performed for 2 hours. After the digestion reaction, the solution was subjected to 0.8% (w / v) agarose gel electrophoresis to purify a DNA fragment of about 1.2 Kb containing a diphtheria toxin expression kit.
- RsrII-RsrII fragment (about 1.2 Kb) derived from plasmid pKOSelectDT obtained above ⁇ . ⁇ 1, Rsrll-Rsrll fragment derived from plasmid pK0FUT8gE2-5 (about 10.4 Kb) 1.01, sterilized water 3 .0 ⁇ l and Ligation High (manufactured by Toyobo Co., Ltd.) 5.01 were mixed and allowed to react at 16 ° C. for 30 minutes to perform a binding reaction. Escherichia coli DH5 strain was transformed using the reaction solution, and plasmid DNA was isolated from the obtained ampicillin-resistant clones according to a known method. This plasmid hereinafter referred to as P K0FUT8Puro.
- the gene transfer of plasmid pK0FUT8Puro into strain 5-03 was carried out according to the electroporation method [Cytotechnology, 3, 133 (1990)] according to the following procedure. went. First, 150 ⁇ g of plasmid pK0FUT8Puro is dissolved in 1.8 ml of NEBuffer for Sail (New England Biolabs), and 600 units of restriction enzyme ⁇ JJ (New England Biolabs) are added for 5 hours at 37 ° C. It was linearized by performing a digestion reaction. The reaction solution was subjected to phenol / chloroform extraction treatment and ethanol precipitation, and the recovered linearized plasmid was used as an aqueous solution.
- 5 - 03 Strain K-PBS buffer (137mraol / l KC1, 2. 7mmol / l NaCl, 8. lmmol / 1 Na 2 HP0 have 1. 5mmol / l KH 2 P0 4 , 4. Ommol / 1 MgCl 2 ) to 8 ⁇ 10 7 cells / ml.
- Cell suspension 200 1 after the (1.
- the cells were suspended in IMDM medium (manufactured by Life Technologies) and seeded on 30 10-cm dishes for adherent cell culture (manufactured by Falcon). 5% C0 2, 37 After culturing for 24 hours under the conditions of ° C, the culture supernatant was removed and, 15 ⁇ g / mlPuromycin (SIGMA Co.) and 10% ⁇ shea dialyzed fetal serum (Life Technologie, Inc.) 10 ml of the added IMDM medium (manufactured by Life Technologies) was dispensed. The culture medium was exchanged every 3 to 4 days, and cultivation was performed for 10 days to obtain a Pyuguchi mycin-resistant strain.
- IMDM medium manufactured by Life Technologies
- the culture supernatant was removed from the 10 cm dish in which the puromycin-resistant strain appeared, and 7 ml of a phosphate buffer solution was injected, followed by transfer under a stereoscopic microscope.
- a pipette man GILS0N
- the colony was picked up and sucked, and collected into a round-bottom 96-well plate (Falcon).
- each clone was seeded on a flat-bottom 96-well plate for adherent cells (manufactured by Iwaki Glass Co., Ltd.), and 15 / ⁇ g / ml Puromycin (manufactured by SIGMA) and 10% fetal dialysis serum (Ufe It was cultured for 1 week using IMDM medium (manufactured by TECH) supplemented with IMDM medium (manufactured by Life Technologies). After culturing, each clone on the plate was trypsinized and mixed with twice the amount of a freezing medium (20% DMSO, 40% fetal serum, 40% IMDM).
- Replica plates were prepared by culturing for 1 week in IMDM medium (Life Technologies) supplemented with 15 / ig / ml Puromycin (SIGMA) and 10% fetal dialysis serum (Life Technologies). did.
- Diagnosis of homologous recombination by genomic PCR was performed on the 900 clones obtained in this section (2) according to the following procedure.
- genomic DNA of each clone was prepared from the replica plate prepared in this section (2) according to a known method [Analytical Biochemistry, 201, 331 (1992)]. This was dissolved in 30 ⁇ l of TE-RNase buffer (pH 8.0) (10 ml / l Tris-HC1, lramol / 1 EDTA, 200 ⁇ g / ml RNase A).
- TE-RNase buffer pH 8.0
- a primer shown in SEQ ID NO: 15
- a primer that binds to the 16 ⁇ sequence in the vector Shown below).
- the reaction solution 25 / zl each containing 10 1 genomic DNA dissolved solution prepared above [ExTaq buffer (manufactured by Takara Shuzo), 0.2 negation ol / l dNTPs, 0 .5 / iniol / l
- the above gene-specific primers (SEQ ID NO: 15 and SEQ ID NO: 16) were prepared and subjected to polymerase chain reaction (PCR).
- the PCR was performed under the conditions of heating at 94 ° C for 3 minutes, followed by 38 cycles in which a single reaction consisting of 94 ° C for 1 minute, 60 ° C for 1 minute, and 72 ° C for 2 minutes was performed. .
- reaction mixture was subjected to 0.8% (w / v) agarose gel electrophoresis, and a specific amplification of about 1.7 Kb including the border between the CH0 cell genomic region and the targeting vector homologous region was observed. Those were designated as positive clones. One positive clone was found by this method.
- This section (3) One clone positive in this section (3) was diagnosed by a genomic Southern blot for homologous recombination by the following procedure. Among the master plates stored by cryopreservation in the above (2), this section select 96 well plates containing positive clones found in (3), 5% C0 2 , 37 ° 10 min under the conditions of C It was left still. After standing, cells were seeded from a well corresponding to a positive clone into a flat bottom 24-well plate for adherent cells (Greiner).
- the genome is transferred to a nylon membrane according to a known method [Procedures 'Ob' 'The National' Academy 'Off,' Science (Proc. Natl. Acad. Sci. USA), 76, 3683 (1979)].
- the DNA was transcribed. After the completion of the transfer, a heat treatment was performed on the Ni-nickel film at 80 ° C for 2 hours.
- a probe used for Southern blot was prepared as follows. First, primers (SEQ ID NO: 9 and SEQ ID NO: 10) were designed to bind to the sequence of the FUT8 genomic region obtained in the reference example that exceeded the targeting vector homologous region. Next, using DNA polymerase ExTaq (Takara Shuzo), 20 ⁇ l of a reaction solution [ExTaq buffer (Takara Shuzo) containing plasmid P FUT8fgE2-2 4. Ong obtained in Reference Example (2), 0.
- the obtained probe DNA solution (5 ⁇ l) was radiolabeled using [ ⁇ - 32 P] dCTP 1.75 MBq and Megaprime DNA Labeling system, dCTP (Amersham Pharmacia Biotech).
- the hybridization was performed as follows. First, the above nylon membrane was sealed in a bottle, and hybridization solution [5 X SSPE, 50 X Denhaldt's solution, 0.5% (w / v) SDS, 100 II g / ml salmon sperm DNA] was added and prehybridization was performed at 65 ° C for 3 hours. Next, the 32 P-labeled probe DNA was heat-denatured and put into a bottle, and heated at 65 ° C. for 1 B.
- the nylon membrane was immersed in 50 ml of 2 ⁇ SSC-0.1% (w / v) SDS and heated at 65 ° C. for 15 minutes. After repeating the above washing operation twice, the membrane was immersed in 50 ml of 0.2 ⁇ SSC-0.1% (w / v) SDS, and heated at 65 ° C. for 15 minutes. After washing, the nickel film was exposed to X-ray film at ⁇ 80 ° C. and developed.
- the Cre recombinase expression vector pBS185 (manufactured by Life Technologies) was introduced into the 1st. AFUT82 2-46 strain prepared in Section 2 of this Example.
- Plasmid pBS185 was introduced into the 1st. AFUT82 2-46 strain by the following procedure according to the electroporation method [Cytotechnology, 3, 133 (1990)].
- 1st. AFUT8 the 2-46 strain K-PBS buffer [137 ⁇ ol / l KC1, 2. 7 thigh ol / l NaCl, 8. lmmol / 1 Na 2 HP0 4, 1. 5 Yuzuru ol / l KH 2 P0 4, was 4. 0 mmol / l suspended in MgCl 2] Nigoshi to 8 X 10 7 cells / ml. After the cell suspension 200 ⁇ ⁇ the (1.
- the culture supernatant was removed from a 10-cm dish, 7 ml of a phosphate buffer was injected, and then transferred under a stereoscopic microscope.
- colonies were picked up using a pipetman (manufactured by GILSON), sucked, and collected into a round-bottom 96-well plate (manufactured by Falcon).
- trypsinization each clone was seeded on a flat-bottomed 96-well plate for adherent cells (manufactured by Iwaki Glass), and IMDM medium (manufactured by Life Technologies) supplemented with 10% fetal dialysis serum (manufactured by Life Technologies) ) For 1 week.
- each clone on the plate was trypsinized, and mixed with twice the amount of a freezing medium (20% DMSO, 40% fetal serum, 40% IMDM). One half of this was seeded on a flat-bottom 96-well plate for adherent cells (Iwaki Glass Co., Ltd.) to prepare a replica plate, and the other half was frozen and stored as a master plate.
- a freezing medium (20% DMSO, 40% fetal serum, 40% IMDM).
- Diagnosis of drug-resistance gene elimination was performed by genomic Southern blot on any 6 of the positive clones found in this section (2) by the following procedure.
- Genomic DNA of each clone was prepared from the plate according to a known method [Nucleic Acids Research, 3, 2303 (1976)], and ⁇ -RNase buffer (pH 8.0) (I0 mmol / 1 Tris-HC1, lmmol / 1 EDTA, 200 ⁇ g / ml RNase A) dissolved in 150 ⁇ l.
- the sample is transferred to a nylon membrane according to a known method [Procedures 'Ob' 'The' National 'Academi 1' Ob Science (Proc. Natl. Acad. Sci. USA), 76, 3683 (1979)].
- Genomic DNA was transcribed. After the completion of the transfer, a heat treatment was performed on the nip film at 80 ° C for 2 hours.
- a probe used for the Southern plot was prepared as follows. First, primers (SEQ ID NO: 9 and SEQ ID NO: 10) were designed to bind to the sequence of the FUT8 genomic region obtained in the reference example that exceeded the targeting vector homologous region. Next, using a DNA polymerase ExTaq (Takara Shuzo), 20 ⁇ l of the reaction solution [ExTaq buffer (Takara Shuzo), containing the plasmid pFUT8fgE2-2 4. Ong obtained in Reference Example (2), 0.2 The original gene-specific primers (SEQ ID NO: 9 and SEQ ID NO: 10) were prepared and subjected to polymerase chain reaction (PCR).
- PCR polymerase chain reaction
- PCR was performed at 94 ° C for 1 minute, followed by 25 cycles of one cycle consisting of 94 ° C for 30 seconds, 55 ° C for 30 seconds, and 74 ° C for 1 minute. .
- the reaction solution was subjected to 1.75% (w / v) agarose gel electrophoresis to purify a probe DNA fragment of about 230 bp. 5 ⁇ l of the obtained probe DNA solution was radioactively labeled using [ ⁇ - 32 P] dCTP 1.75 MBq and Megaprime DNA Labeling system, dCTP (Amersham Pharmacia Biotech).
- the hybridization was performed as follows. First, the above nylon membrane was sealed in a bottle, and hybridization solution (5 X SSPE, 50 X Denhaldt's solution, 0.5% (w / v) SDS, 100 ⁇ g / ml salmon sperm) DNA) and add 3 ml at 65 ° C for 3 hours. A pre-hyperlysis was performed. Next, the probe DNA labeled with 32 P was heat-denatured and put into a bottle, followed by heating at 65 ° C. for a while.
- hybridization solution 5 X SSPE, 50 X Denhaldt's solution, 0.5% (w / v) SDS, 100 ⁇ g / ml salmon sperm) DNA
- the nylon membrane was immersed in 50 ml of 2 XSSC-0.1% (w / v) SDS and heated at 65 ° C for 15 minutes. After repeating the above washing operation twice, the membrane was immersed in 50 ml of 0.2 ⁇ SSC-0.1% (w / v) SDS, and heated at 65 ° C. for 15 minutes. After washing, the nickel film was exposed to X-ray film at ⁇ 80 ° C. and developed.
- Treatment with the restriction enzyme BamHI described above yields a DNA fragment of approximately 25.5 Kb from the wild-type FUT8 allele.
- a DNA fragment of about 20.0 Kb is generated from the allele that has undergone homologous recombination with the targeting vector by the same restriction enzyme treatment.
- the puromycin resistance gene about 1.5 Kb is removed from the homologous allele, a DNA fragment of about 18.5 Kb is generated by the same treatment.
- FIG. 12 shows the results of the genomic Southern of the above-mentioned 1st. AFUT8 2-46-1, 1st. AFUT82 2-46 and 5-03 strains. 1st.
- AFUT8 2-46-1 is the name of 2-46-1, and was filed on September 26, 2001 by the National Institute of Advanced Industrial Science and Technology (AIST), Patent Organism Depositary 1 (Higashi 1, Tsukuba, Ibaraki, Japan). Deposited as FERM BP-7755 at Chome No. 1 Central No. 6).
- the 1st. AFUT8 2-46-1 strain in which one copy of the FUT8 allele obtained in Section 3 of the present example was torn, was transformed with a 10% fetal dialysis serum (Life) at a density of 3 ⁇ 10 5 cells / ml.
- IMDM medium Le Technologies
- a total of 60 ml each was seeded on two T182 flasks for adhesion cell culture (Greiner). After culturing for 3 days, the supernatant was removed and replaced with a total of 60 ml of EXCELL301 medium (manufactured by JRH Biosciences).
- a 0.8 cm diameter column is filled with Prosep-A HighCapacity (manufactured by bioPROCESSING) to a thickness of 2 cm, 10 ml of 0.1 mol / 1 citrate buffer (pH 3.0) and 10 ml of lmol / 1 glycine / NaOH-0.15 mol
- the carrier was washed by washing with 10 ml of / l NaCl buffer (pH 8.6) sequentially.
- 100 ml of each of the above culture supernatants was passed through the column, and washed with 50 ml of 1 mol / 1 glycine / NaOH-0.15 mol / l NaCl buffer (pH 8.6).
- ADCC activity was measured using the CCR4-positive cell line CCR4 / EL-4 described in W001 / 6475.
- RPMI1640-FBS 10% fetal serum
- the pieces were suspended in RPMI1640- FBS (10) 500, by culturing 90 minutes by the addition of Na 2 51 Cr0 4 3. 7MBq 37 ° C, it was radiolabeled cells. After centrifugation at 1200 rpm for 5 minutes, the supernatant was removed, and the labeled cells were suspended in 5 ml of RPMI1640-FBS (IO). After repeating this washing operation three times, the cell suspension was allowed to stand on ice for 30 minutes to spontaneously dissociate radioactive substances. After the above washing operation was repeated twice, the target cells were suspended in RPMI1640-FBS (10) 5ral to prepare 2.0 ⁇ 10 5 cells / ml of the target cell suspension.
- RPMI1640-FBS 10 ml
- an effector cell suspension of 2.5 ⁇ 10 6 cells / ral was prepared using RPMI1640-FBS (IO).
- the target cell suspension was dispensed into each well of a 96-well U-shaped bottom plate (manufactured by Falcon) in an amount of 50 ⁇ l (1 ⁇ 10 4 cells / well). Subsequently, the effector-cell suspension was dispensed into each well at a ratio of 25 ⁇ l (2.5 ⁇ 10 5 cells / well) to give a ratio of effector-cell to target cell of 25: 1.
- RPMI1640-FBS (10) the anti-CCR4 antibodies obtained in Section 4 of this Example The 0.
- liig / ml, 1 / g / ml, 10 g / ml dilution series was prepared by adding 50 ⁇ 1 each Ueru of the diluted solvent solution, final concentration 0.00 25 ⁇ / ⁇ 1, 0.025 ig / ml, 0.25 tg / ml and 2.5 / _tg / ml. After reacting under conditions of 5% CO 2 and 37 ° C. for 4 hours, the plate was centrifuged at 1200 rpm for 5 minutes.
- the value of the amount of spontaneously dissociated 51 Cr was determined by performing the above reaction in a system in which 150 ⁇ l of RPMI1640-FBS (10) was added instead of the effector cell suspension and the antibody solution. Further, by performing the above reaction in a system to which 100 ⁇ l of 1N hydrochloric acid and 50 ⁇ l of RPMI1640-FBS (10) were added instead of the effector cell suspension and antibody solution, the value of total dissociated 51 Cr was calculated. I asked. Using these values, the ADCC activity was determined by the formula (I) described in Example 2 (3).
- FIG. 13 shows ADCC activities of various anti-CCR4 antibodies.
- One copy of the FUT8 allele The antibody composition obtained from the 1st. AFUT8 2-46-1 strain that had been torn was significantly higher in ADCC than the antibody composition produced by the CH0 cell strain 5-03 before the gene disruption. Showed activity. No change was observed in the antigen binding activity of these antibody compositions. From the above results, it has been clarified that by disrupting the FUT8 allele in the host cell, the ADCC activity of the produced antibody composition can be increased.
- Example 4 Acquisition of highly drug-resistant strain from CH0 cells in which one copy of FUT8 gene has been disrupted
- the concentration of the drug for positive selection used to select the target vector-inserted strain in cells in which one of the genomic genes obtained by homologous recombination obtained by the homologous recombination method using the target vector was selected was increased about 10-fold. It is generally known that by culturing in a medium and obtaining a strain highly resistant to the drug, a strain in which both aryls are broken can be obtained (manipulating 'the' mouse 'embryo) .A'lab Laury-Ma-Yuanore, Gene Targeting, A Practical Approach, IRL Press at Oxford University Press (1993), Production of Mutant Mice Using ES Cells).
- a FUT8 2-46 1 ⁇ 10 8 cells were suspended in IMDM medium (Life Technologies) supplemented with 10% fetal dialysis serum (Life Technologies), and 10 cm dishes for adherent cell culture (Falcon After seeding on 20 sheets, the cells were cultured for 24 hours under the conditions of 5% CO 2 and 37 ° C. After culturing, the supernatant was removed, 150 ⁇ ⁇ / ⁇ 1 puromycin (SIGMA Co.) and 10% ⁇ shea dialyzed fetal serum (Life Technologie, Inc.) was added and the IMDM culture place a (Life Technologies Inc.) by 10ml Dispensed. The culture was performed for 12 days while repeating this medium exchange operation every 3 to 4 days.
- each clone was inoculated, 150 ⁇ ⁇ / ⁇ 1 Puromycin ( SIGMA Co.) and 10% ⁇ shea dialyzed fetal serum (Life Technologie (Life Technologies) supplemented with IMDM medium (Life Technologies) for 1 week. After the culture, each clone on the above plate was treated with trypsin, and mixed with twice the amount of the freezing medium [20% DMSO, 40% fetal serum, 40% IMDM].
- Replica plates consist of 15 ig / ml puromycin (Sigma) and 10% fetal dialysis serum.
- the cells were cultured for 1 week in IMDM medium (Ufe Technologies) supplemented with (Life Technologies). (2) Diagnosis of homologous recombination by genome Southern blot
- the hybridization was performed as follows. First, the above nylon membrane is sealed in a bottle with a bottle, and the hybridization solution [5 X SSPE, 50 X Denhaldt's Liquid, 0.5 ° /. (w / v) 15 ml of SDS, 100 ⁇ g / ml salmon sperm DNA] was added, and prehybridization was performed at 65 ° C. for 3 hours. Next, the 32 P-labeled probe DNA was heat-denatured and put into a bottle, and heated at 65 ° C. for one Bj &.
- the membrane was immersed in 50 ml of 2 ⁇ SSC—0.1 l ° / o (w / v) SDS and heated at 65 ° C. for 15 minutes. After repeating the above washing operation twice, the membrane was immersed in 50 ml of 0.2 X SSC-0. L ° / o (w / v) SDS, and heated at 65 ° C for 15 minutes. After washing, the nylon film was exposed to X-ray film at ⁇ 80 ° C. and developed.
- a CH0 / DG44 cell line was constructed in which the genomic region containing the translation initiation codons of both FUT8 alleles was deleted.
- plasmid pK0FUT8Puro 1 O wg is dissolved in 50 ⁇ l of NEBuf fer4 (New England Biolabs), digested with 37 units of restriction enzyme 1 ⁇ 2 £ 1 (New England Biolabs) and digested at 37 ° C for 2 hours. The reaction was performed.
- Ascl-Ascl fragment (about 1.6 Kb) derived from plasmid pKOSelectNeo (about 1.6 Kb) 1.0 ⁇ 1, Ascl-Ascl fragment (about 10.1 Kb) derived from plasmid pK0FUT8Puro 1.0 ⁇ 1, sterile water 3.0 1
- Ligation High (manufactured by Toyobo Co., Ltd.) 5.0 ⁇ 1 was mixed and reacted at 16 ° C. for 30 minutes to perform a binding reaction.
- E. coli DH5 a strain was transformed using the reaction solution, each plasmid DNA according to a known method from the obtained ampicillin-resistant clones were isolated. This plasmid is hereinafter referred to as pK0FUT8Neo.
- the Chinese hamster FUT8 genome constructed in section 1 of this Example was used for Chinese hamster ovary-derived CH0 / DG44 cells lacking the dihydrofolate reductase gene (dhfr) [Somatic Cell and Moleculer Genetics, 12, 555 (1986)].
- the region targeting vector pKOFUTSNeo was introduced.
- the reaction solution was subjected to phenol / chloroform extraction and ethanol precipitation, and the recovered linearized plasmid was used as a 1 ⁇ 8 / ⁇ 1 aqueous solution.
- the CH0 / DG44 cells K-PBS buffer [137mmol / l KC1, 2. 7mmol / l NaCl, 8. lmmol / 1 Na 2 HP04, 1. 5ramol / l KH 2 P0 4, 4. 0mmol / l MgCl 2 ] to 8 ⁇ 10 7 cells / ml. After the cell suspension 200 / xl of (1.
- IMDM medium manufactured by Invitrogen
- 600 ⁇ g / ml G418 manufactured by Nacalai Tesque
- 1-fold concentration of HT supplement manufactured by Invitroge
- 10% fetal calf serum manufactured by Invitrogen
- genomic DNA of each clone is prepared according to a known method [Analytical Biochemistry, 201, 331 (1992)], and use TE-RNase buffer (pH 8. 0) [I0mmol / 1 Tris-HC1, lramol / 1 EDTA, 200 ⁇ g / ml RNase A] Dissolved in 30 ⁇ .
- a primer (SEQ ID NO: 13 or SEQ ID NO: 15) that binds to the sequence beyond the targeting vector homologous region in the FUT8 genomic region obtained in the reference example and a primer (SEQ ID NO: 14 or SEQ ID NO: Using 16), the following polymerase chain reaction (PCR) was performed.
- reaction solution of 25 ⁇ 1 containing 10 11 each of the genomic DNA solution prepared above [DNA polymerase ExTaq (Takara Shuzo), ExTaq buffer (Takara Shuzo), 0.2 mmol / l dNTPs, 0.5 / x mol / l
- the above gene-specific primer forward primer is SEQ ID NO: 13 or SEQ ID NO: 15, lipper primer is SEQ ID NO: 14 or SEQ ID NO: 16
- PCR was performed under the conditions that a cycle consisting of a reaction consisting of 94 ° C for 1 minute, 60 ° C for 1 minute, and 72 ° C for 2 minutes was defined as one cycle.
- IMDM medium Invitrogen
- 600 ig / ml G418 Nacalai Tesque
- 1x HT supplement Invitrogen
- 10% fetal calf serum Invitrogen
- Genomic DNA of each clone was prepared from the plate according to a known method [Nucleic Acids Research, 3, 2303, (1976)], and TE-RNase buffer (pH 8.0) [10 mmol / l Tris-HC1, lramol / 1 EDTA ⁇ 200 ⁇ g / ml RNase A] 1501 was dissolved overnight.
- the genomic DNA is transferred to a nylon membrane according to a known method [Procedures of the 'National' Kir 'of the Science (Pro Natl. Acad. Sci. USA), 76, 3683, (1979). Transcribed. After the transfer was completed, a heat treatment was performed on the Ni-nickel film at 80 ° C for 2 hours.
- a probe used for Southern blot was prepared as follows. First, the following primers (SEQ ID NO: 9 and SEQ ID NO: 10) were used to bind to the part of the FUT8 genomic region obtained in Reference Example that exceeded the targeting vector homologous region. PCR was performed.
- the reaction solution [DNA polymerase ExTaq (Takara Shuzo), ExTaq buffer (Takara Shuzo), 0.2-arm ol / 20 ⁇ containing the plasmid P FUT8fgE2-4 Ong obtained in Reference Example (2) l dNTPs, 0.5 / mol / l Prepare the above gene-specific primers (SEQ ID NO: 9 and SEQ ID NO: 10)], heat at 94 ° C for 1 minute, and then heat at 94 ° C for 30 seconds, 55 ° C The PCR was performed under 25 cycles of 30 seconds at 74 ° C. for 1 minute. After PCR, the reaction solution was subjected to 1.75% (w / v) agarose gel electrophoresis to purify a probe DNA fragment of about 230 bp. For the obtained probe DNA solution 51,
- [A - 32 P] dCTP 1. 75MBq and Megaprime DNA Labeling system, and radiolabeled using dCTP (Amersham Pharmacia Biotech Inc.).
- the hybridization was performed as follows. First, the above nylon membrane was sealed in a bottle with a bottle, and the hybridization solution [5X SSPE, 50X Denhaldt's solution, 0.5 ° /. (w / v) 15 ml of SDS, 100 ⁇ g / ml salmon sperm DNA] was added, and prehybridization was performed at 65 ° C. for 3 hours. Next, the 32 P-labeled probe DNA was heat-denatured and put into a bottle, which was then heated at 65 ° C. for a while.
- the membrane was immersed in 50 ml of 2 ⁇ SSC—0.1% (w / v) SDS and heated at 65 ° C. for 15 minutes. After repeating the above washing operation twice, the membrane was immersed in 50 ml of 0.2 ⁇ SSC-0. 1% (w / v) SDS, and heated at 65 ° C. for 15 minutes. After washing, the nylon film was exposed to an X-ray film at -80 ° C and developed.
- Example 3 The Chinese nomster FUT8 genomic region targeting vector pK0FUT8Puro constructed in Example 3, Item 1 was introduced into the FUT8 minockout clone 50-10-104 obtained in Example 2, Item 2.
- the gene transfer of plasmid pK0FUT8Puro into 50-10-104 was carried out according to the electroporation method [Cytotechnology, 3, 133 (1990)] according to the following procedure.
- the plasmid pK0FUT8Puro U0 ⁇ g was dissolved in 2.4 ml of NEBuffer for Sal I (manufactured by New England Biolabs) containing 100 / zg / ml BSA (manufactured by New England Biolabs), and 800 units of the restriction enzyme Sail ( (New England Biolabs) and a digestion reaction at 37 ° C. for 5 hours for linearization.
- the reaction solution was subjected to a phenol / mouth opening form extraction treatment and ethanol precipitation, and the collected linearized plasmid was used as a 1 ⁇ g / 1 aqueous solution.
- 50 - 10 104 K-PBS buffer [137mraol / l KC1, 2. 7mraol / l NaCl, 8. lmraol / 1 Na 2 HP04, 1. 5mmol / l KH 2 P0 4, 4. 0mraol / l Suspended in MgClJ to 8 ⁇ 10 7 cells / ml. After the cell suspension 200 il of (1.
- Diagnosis of homologous recombination was performed on the drug-resistant clones obtained in this section (1) by genomic Southern plot according to the following procedure.
- the culture supernatant was removed from the 10 cm dish in which the puromycin-resistant clone appeared, and 7 ml of phosphate buffer was injected, and then transferred under a stereoscopic microscope. Next, use a pipette man (GILS0N) to remove the colonies and suck them into a round bottom 96-well plate.
- a pipette man GILS0N
- each clone on the plate was trypsinized and seeded on a flat-bottomed 24-well plate for adherent cells (Greiner).
- IMDM medium Invitrogen
- SIGMA lS ⁇ g / ml puromycin
- 1x HT supplement Invitrogen
- 10% fetal fetal serum Invitrogen
- genomic DNA of each clone was prepared according to a known method [Nucleic Acids Research, 3, 2303, (1976)], and TE-RNase buffer (pH 8.0) [10 mmol / l 1 Tris-HC1, lmmol / 1 EDTA, 200 ⁇ g / ml RNase A] was dissolved in 150 ⁇ l.
- genomic DNA is transferred to a nylon membrane according to a known method [Procedures' ob '' the 'National' Academy 'ob' Science (Pro Natl. Acad. Sci. USA), 76, 3683, (1979). did. After the transfer was completed, a heat treatment was performed on the Ni-nickel film at 80 ° C for 2 hours.
- a probe used for Southern blot was prepared as follows. First, the following PCR was performed using primers (SEQ ID NO: 11 and SEQ ID NO: 12) that bind to the sequence of the FUT8 genomic region beyond the targeting vector homologous region.
- a reaction solution of 20 ⁇ 1 containing the plasmid pFUT8fgE2-4 Ong obtained in Reference Example (2) [DNA polymerase ExTaq (Takara Shuzo), ExTaq buffer (Takara Shuzo), 0.2 bandol ol / l dNTPs, 0.5 ⁇ 1 / 1
- PCR was performed under the conditions of 25 cycles, in which a reaction consisting of 1 minute at 74 ° C. for 1 second was performed.
- the reaction solution was subjected to 1.75% (w / v) agarose gel electrophoresis to purify a probe DNA fragment of about 230 bp.
- 5 ⁇ l of the obtained probe DNA solution was radiolabeled using [Hi- 32 P] dCTP (1.75 MBq of Megaprime DNA Labeling system, dCTP manufactured by Amershara Pharmacia Biotech). The high predication is performed as follows.
- the above nylon membrane was sealed in a bottle with a bottle, and a hybridization solution [5 X SSPE, 50 X Denhaldt's solution, 0.5% (w / v) SDS, 100 tg / ml salmon sperm DNA] 15 ml , And prehybridization was performed at 65 ° C for 3 hours.
- the probe DNA labeled with 32 P was heat-denatured and put into a bottle, and heated at ⁇ 65 ° C.
- the membrane was immersed in 50 ml of 2 ⁇ SSC—0.1% (w / v) SDS and heated at 65 ° C. for 15 minutes. After the above washing operation was repeated twice, the membrane was immersed in 50 ml of 0.2 ⁇ SSC-0. L% (w / v) SDS, and heated at 65 ° C. for 15 minutes. After washing, apply nylon membrane to X-ray film -80. Exposure was carried out with C and developed.
- the cells suspension was 200 1 (1.
- Arbitrary clones were collected from the colonies obtained by introducing the gene into WK704 by the following procedure. First, the culture supernatant was removed from a 10 cm dish, 7 ml of phosphate buffer was injected, and then transferred under a stereoscopic microscope. Next, using a pipetteman (GILS0N), the colonies were picked up and sucked, and collected into a round-bottom 96-well plate (Falcon). After trypsinization, each clone was seeded on a flat-bottomed 96-well plate for adherent cells (manufactured by Iwaki Glass Co., Ltd.). The cells were cultured for one week using an IMDM medium (manufactured by Invitrogen) supplemented with.
- IMDM medium manufactured by Invitrogen
- each clone on the above plate was treated with trypsin, and mixed with twice the amount of the freezing medium [20% DMSO, 40% fetal serum, 40% IMDM]. Half of this was seeded on a flat-bottom 96-well plate for adherent cells (Iwaki Glass) to make a replica plate, and the other half was frozen and stored as a master plate.
- the replica plate was diluted with 600 / g / ml G418 (Nacalai Tester), 15 ⁇ g / ml puromycin (SIGMA), 10% fetal serum (Invitrogen) and 1x HT supplement ( The cells were cultured for 7 days using IMDM medium (Invitrogen) supplemented with Invitrogen. Positive clones in which the drug resistance genes on both alleles sandwiched between the ⁇ sequences have been removed by the expression of Cre recombinase die in the presence of G418 and puromycin. Positive clones were found by this negative selection method.
- a 96-well plate containing the above positive clones was selected and allowed to stand for 10 minutes at 5% CO 2 and 37 ° C. After standing, cells from the wells corresponding to the above clones were transferred to a flat-bottom 24-well plate for adherent cells (Greiner Seeds). After culturing for 1 week in IMDM medium (Invitrogen) supplemented with 10% fetal serum (Invitrogen) and 1-fold HT supplement (Invitrogen), a flat bottom 6-well plate for adherent cells ( Greiner).
- Genomic DNA of each clone was prepared from the plate according to a known method [Nucleic Acids Research, 3, 2303, (1976)], and TE-RNase buffer (pH 8.0) [10 mmol / l Tris-HC1, lmmol / 1 EDTA, 200 ⁇ g / ml RNase A] was dissolved in 150 ⁇ l.
- the genomic DNA is transferred to a nylon membrane according to a known method [Procedin's' ob '' the 'Nashinanore' academy 'ob' Science (Proc. Natl. Acad. Sci. USA), 76, 3683, (1979). Was transcribed. After the transfer was completed, the thin film was heat-treated at 80 ° C for 2 hours.
- a probe used for the Southern plot was prepared as follows. First, the following PCR was performed using primers (SEQ ID NO: 11 and SEQ ID NO: 12) that bind to the sequence of the FUT8 genomic region beyond the targeting vector homologous region.
- the reaction solution was subjected to 1.75% (w / v) agarose gel electrophoresis to purify a probe DNA fragment of about 230 bp. 5 ⁇ l of the obtained probe DNA solution was radiolabeled using [ ⁇ - 32 P] dCTP 1.75 MBq and Megaprime DNA Labeling system, dCTP (manufactured by Amersnam Pharmacia Biotech).
- the hybridization was performed as follows. First, the above nylon membrane was sealed in a bottle, and a hybridization solution [5 X SSPE, 50 X Denhaldt, s solution, 0.5% (w / v) SDS, 100 ⁇ g / ml salmon sperm DNA] Add 15 ml and press at 65 ° C for 3 hours. Re-hyperidization was performed. Next, the probe DNA labeled with 32 P was heat-denatured and put into a bottle, and heated at ⁇ 65 ° C.
- the membrane was immersed in 50 ml of 2 ⁇ SSC—0.1% (W / V) SDS and heated at 65 ° C. for 15 minutes. After repeating the above washing operation twice, the membrane was immersed in 50 ml of 0.2 X SSC-0.1% (W / V) SDS and heated at 65 ° C for 15 minutes. After washing, the nylon film was exposed to an X-ray film at -80 ° C and developed.
- the Nhel treatment with the restriction enzyme described above generates a DNA fragment of approximately 8.0 Kb from the wild-type FUT8 allele.
- a DNA fragment of about 9.5 Kb was generated from the allele that had undergone homologous recombination with the targeting vector by the same restriction enzyme treatment.
- the neomycin resistance gene about 1.6 Kb
- the pure bitemycin resistance gene about 1.5 Kb
- the genomic DNA of the tested positive clone 4-5-C3 (4 (3) positive clone in Example 5) was homologous recombination region-specific with the drug-resistant gene removed.
- a cDNA encoding the amino acid sequence of the VL of the anti-CD20 mouse monoclonal antibody 2B8 described in W094 / 11026 was constructed by PCR as follows.
- the 5'-end and 3'-end of the nucleotide sequence of VL described in W094 / 11026, the binding nucleotide sequence of the primer for amplification during PCR reaction (restriction enzyme recognition sequence for cloning into a humanized antibody expression vector) ).
- six synthetic DNAs of SEQ ID NOs: 19, 20, 21, 22, 23, and 24 were prepared (consigned to GENSET) in the alternate order of the antisense strand.
- Each oligonucleotide was adjusted to a final concentration of 0.1 / M with 50 reaction mixtures [PCR Buffer # 1 with K0D DNA Polymerase (Toyobo), 0.2raM dNTPs, IraM Gnesium, 0.5 ⁇ M13 primer M4 (Takara Shuzo), 0.5 / M M13 primer RV (Takara Shuzo)] and DNA thermal cycler GeneAmp PGR System 9600
- a cDNA encoding the amino acid sequence of VH of the anti-CD20 mouse monoclonal antibody 2B8 described in W094 / 11026 was constructed by PCR using the following method.
- the 5'-end and 3'-end of the nucleotide sequence of VH described in TO94 / 11026, the binding base sequence of the amplification primer during the PCR reaction (the restriction enzyme recognition sequence for cloning into a human antibody expression vector is also included). Included). Divide the designed base sequence into a total of 6 base sequences of about 100 bases from the 5 'end side (adjacent base sequences should have an overlapping sequence of about 20 bases at their ends), Actually, six synthetic DNAs of SEQ ID NOS: 25, 26, 27, 28, 29, and 30 were prepared (consigned to GENSET) in the alternate order of the antisense strand.
- Escherichia coli DH5a strain (manufactured by Toyobo Co., Ltd.) was transformed using the recombinant plasmid DNA solution thus obtained.
- Each plasmid DNA was prepared from the clone of the transformant, and reacted using BigDye Terminator Cycle Sequencing Ready Reaction Kit v2.0 (manufactured by Applied Biosystems) according to the attached instructions, followed by base sequencing using the DNA sequencer ABI PRISM 377 of the company. The sequence was analyzed. Thus, the plasmid pBS-2B8H shown in FIG. 20 having the desired nucleotide sequence was obtained.
- reaction mixture containing lng of the above plasmid pBS-2B8H [LA PCR Buffer II (Takara Shuzo), 2.5 units of TaKaRa LA Taq, 0.4 mM dNTPs, 2.5 mM magnesium chloride, 50 nM T3 BcaBEST Sequencing primer (Takara Shuzo Co., Ltd.) and 50 nM of the above primer for mutation introduction (SEQ ID NO: 31, GENSET Co.) were prepared, and DNA thermal cycler was performed using GeneAmp PCR System 9600 (Perkin Elmer). A cycle of 30 seconds at 55 ° C, 2 minutes at 55 ° C, and 1 minute 30 seconds at 72 ° C was performed 25 times. After agarose gel electrophoresis of the reaction solution 30, the QIAquick Gel Extraction Kit
- the reaction mixture 30 was agarose gel electric After the electrophoresis, a PCR product of about 0.63 kb was recovered using a QIAquick Gel Extraction Kit (manufactured by QIAGEN) and used as a 30 ⁇ L aqueous solution. Subsequently, 0.45 kb of the PCR product obtained above and 0.63 kb of the PCR product were each divided into 0.5 and 47.5 ⁇ of the reaction solution [LA PCR Buffer II (Takara Shuzo), 0.4 mM dNTPs.
- An expression vector PKANTEX2B8P for a CD20 human chimeric antibody (hereinafter referred to as an anti-CD20 chimeric antibody) was constructed as follows.
- the plasmid pBS-2B8L obtained in this section (1) is reacted at 55 ° C for 1 hour with 10 units of restriction enzyme WI (manufactured by New England Biolabs), and then an additional 10 units of restriction enzyme ⁇ RI (Takara Shuzo) at 37 ° C for 1 hour.
- the reaction solution was fractionated by agarose gel electrophoresis, and a BsiWI-EcoRI fragment of about 0.41 kb was recovered.
- 2 ⁇ g of the humanized antibody expression vector pKANTEX93 was reacted for 1 hour at 55 ° C.
- the resulting plasmid pBS in the item (2) - After reacting for 1 hour at 37 ° C for using 2 mu ⁇ the 10 units of a restriction of 2B8Hm enzyme ⁇ I (Takara Shuzo), further 10 The reaction was carried out at 37 ° C. for 1 hour using enzyme l (Takara Shuzo). The reaction solution was fractionated by agarose gel electrophoresis, and an Apal-Notl fragment of about 0.45 kb was recovered.
- the Apal-Notl fragment derived from the plasmid pBS_2B8Hm and the Apal-Notl fragment derived from the plasmid PKANTEX2B8-L were obtained using Solution I of DNA Ligation Kit Ver. 2 (Takara Shuzo Co., Ltd.). It was linked according to the book. Escherichia coli DH5a strain (manufactured by Toyobo Co., Ltd.) was transformed using the recombinant plasmid DNA solution thus obtained, and each plasmid DNA was prepared from the clone of the transformed strain.
- Example 5 The anti-CD20 antibody expression vector pKANTEX2B8P obtained in the first section of this example was introduced into the double knockout clone WK704 of the FUT8 gene prepared in the third section. Gene transfer of plasmid PKANTEX2B8P into WK704 is performed by electroporation.
- the WK704-2B8P strain is the name of the WK704-2B8P strain, and was set up on March 20, 2003 at the National Institute of Advanced Industrial Science and Technology, Patent Organism Depositary (Central 6th, 1-1-1, Higashi, Tsukuba, Ibaraki, Japan). ) Has been deposited as FERM BP-8337.
- Example 5 The anti-ganglioside GD3 chimeric antibody expression vector plasmid pKANTEX641 was introduced into the FUT8 gene double knockout clone WK704 prepared in Section 3 to prepare a stable anti-GD3 chimeric antibody expression strain.
- PKANTEX641 was prepared using the anti-GD3 chimeric antibody expression vector plasmid pChi641LHGM4 described in TO00 / 61739 and the humanized antibody.
- Working vector PKANTEX93 [(...
- Example 5 The anti-CCR4 chimeric antibody expression vector pKANTEX2160 described in TO01 / 64754 was introduced into the FUT8 gene double knockout clone WK704 prepared in Section 3 to prepare a stable anti-CCR chimeric antibody expression strain.
- the plasmid pKANTEX2B8P was introduced into WK704 by the following procedure according to the electroporation method [Cytotechnology, 3, 133 (1990)]. First, dissolve 15 g of plasmid pKANTEX2160 in 100 ⁇ l of NEBuffer 4 (New England Biolabs), add 40 units of restriction enzyme Aatll (New England Biolabs), and perform digestion reaction at 37 ° C for 2 hours. It was linear.
- the reaction solution was subjected to phenol / chloroform extraction treatment and ethanol precipitation, and the recovered linearized plasmid was used as a lig / ⁇ ⁇ aqueous solution.
- replace WK704 with K-PBS buffer
- the anti-CD20 antibody-expressing strain WK704-2B8P obtained in Section 2 of this Example was transferred into IMDM medium (Invitrogen) supplemented with 10% fetal dialysis serum (Invitrogen) at 3 ⁇ 10 5 cells / ml. After suspension at a density of, a total of 300 ml was seeded on 10 T182 flasks (Greiner) for adherent cell culture. Similarly, the anti-GD3 antibody-expressing strain WK704-2871 obtained in the third section of the present example and the anti-CCR4 antibody expressing strain WK704-2760 obtained in the fourth section of the present example were seeded.
- a 0.8 cm diameter column is filled with 0.5 ml of Mab Select (Araersham Pharmacia Biotech) and purified water 3.0 ml and 0.2 mol / l boric acid 0.15 mol / l NaCl buffer
- ADCC activity was measured according to the following description.
- RPMI1640-FCS 10-10 Cultured human B lymphocyte cell line Raji cells (JCRB9012) cultured in medium (RPI1640 medium containing 10% FCS (GIBC0 BRL)) were centrifuged and suspended to obtain RPMI1640-FCS ( 5) After washing with a medium (RPI1640 medium containing 5% of FCS (manufactured by GIBCO BRL)), the cells were adjusted to 2 ⁇ 10 5 cells / mL with RPMI1640-FCS (5) medium to obtain a target cell solution.
- the plate was centrifuged, and the lactate dehydrogenase (LDH) activity in the supernatant was measured using a CytoTox96 NoN-Radioactive Cytotoxicity Assay (Promega).
- LDH lactate dehydrogenase
- the amount of spontaneously free LDH in target cells was determined by performing the same operation as above using only the medium instead of the effector cell solution and the antibody solution, and measuring the LDH activity of the supernatant.
- the absorbance data of the spontaneous release of the effector cells was obtained by performing the same operation as above using only the medium instead of the target cell solution and the antibody solution.
- the total amount of free LDH due to destruction of all target cells was determined by using a medium instead of the antibody solution and the effecta-one-cell solution, adding the 9% Triton X-100 solution 45 minutes before the end of the reaction, and performing the same procedure as above. It was determined by measuring the LDH activity of the supernatant. Using these values, ADCC activity was determined by the following equation (II).
- ADCC activity (%) X 100 (II)
- FIG. 22 shows the ADCC activity of each anti-CD20 antibody.
- the antibody obtained from the FUT8 gene double knockout clone WK704-2B8P showed higher ADCC activity than commercial Rituxan TM at any antibody concentration, and also had the highest cytotoxic activity value.
- Rituxan TM is an anti-CD20 chimeric antibody produced using CH0 cells in which the FUT8 gene has not been disrupted as host cells.
- the sugar chain analysis of the anti-CD20 antibody, anti-GD3 antibody and anti-CCR4 antibody produced by the FUT8 gene double knockout clone obtained in Example 6 was performed according to the method described in Example 2. (4). Monosaccharide composition analysis was performed on the sugar chain composition of these antibodies according to a known method [Journal of Liquid Chromatography, 6, 1577, (1983)]. As a result, no fucose-containing sugar chain structure was confirmed in any of the antibodies obtained from the FUT8 gene double knockout clone WK704.
- the reaction solution was subjected to 0.8% agarose gel electrophoresis to purify about 2 Kb of the specific amplified fragment. 4 ⁇ l of this DNA fragment was prepared using the T0P0 TA cloning Kit (Invitrogen).
- the plasmid PCR2.1 was introduced according to the instruction manual, and the reaction mixture was used to transform Escherichia coli DH5 strain. Plasmid DNA was isolated from each of the obtained kanamycin-resistant colonies from eight clones in which cDNA was integrated according to a known method.
- the nucleotide sequence of the cDNA inserted into each plasmid was determined using the DNA Sequencer 377 (Parkin Elmer) and the BigDye Terminator Cycle Sequencing FS Ready Reaction Kit (Parkin Elmer). According to the attached manual. By this method, it was confirmed that all the inserted cDNAs encode a sequence including the full length of 0RF of FUT8 in CH0 cells. Among these, a plasmid DNA was selected which did not contain any base reading errors due to PCR in the sequence.
- the present plasmid CHfFUT 8 - called pCR2 1 and..
- the determined nucleotide sequence of the CH0 cell FUT8 cDNA is shown in SEQ ID NO: 1.
- the amino acid sequence is shown in SEQ ID NO: 4.
- CH0 cell FUT80RF full-length cDNA fragment obtained in this section (1) was obtained according to a known genomic screening method described in, for example, Irizu, 'In' Morekiura Ibiology, A Laboratory Manual, 2nd Ed. (1989).
- genomic clone is digested with various restriction enzymes, and Southern hybridization is performed using an AfaI-Sau3AI fragment (about 280 bp) containing the initiation codon of CH0 cell FUT8 cDNA as a probe. Among them, a fragment (about 2.5 Kb) and a Sac-to-Sacl fragment (about 6.5 Kb) were selected and inserted into pBluescriptll KS (+) (Strategene).
- the nucleotide sequence of each genomic fragment obtained was determined using DNA Sequencer-377 (Parkin Elmer) and BigDye Terminator Cycle Sequencing FS Ready Reaction Kit (Parkin Elmer), and the method was in accordance with the attached manual. According to this method, the Xbal-Xbal fragment has a sequence of about 2.5 Kb of the upstream intron containing exon 2 of FUT8 in CH0 cells, and the Sacl-Sacl fragment has a sequence of about 6.5 Kb of the downstream intron containing exon 2 of FUT8 in CH0 cells. It was confirmed that each encoded.
- pFUT8f g E2- 4 plasmids containing plasmid P FUT8fgE2- 2, Sacl-Sacl fragment containing the Xbal-Xbal fragment.
- the determined nucleotide sequence (approximately 9.0 Kb) of the genomic region including exon 2 of FUT8 in CH0 cells is shown in SEQ ID NO: 3.
- the present invention has been designed to reduce or eliminate the activity of an enzyme involved in sugar chain modification in which the 1-position of fucose is ⁇ - linked to the 6-position of N-acetyldarcosamine at the reducing end of N-glycoside-linked complex type sugar chain.
- Cell whose genome is modified, method for producing antibody composition using the cell, transgenic non-human animal or plant produced using the cell, antibody composition produced by the method, and antibody A medicament containing the composition can be provided. Sequence listing free text
- SEQ ID NO: 30 Description of artificial sequence Synthetic DNA
- SEQ ID NO: 31 Description of artificial sequence: Synthetic DNA
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JP (1) | JPWO2003085107A1 (ja) |
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CN (3) | CN1930288B (ja) |
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BR (1) | BR0309145A (ja) |
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EP1498485A4 (en) | 2006-09-06 |
KR20050000380A (ko) | 2005-01-03 |
CN1930288A (zh) | 2007-03-14 |
BR0309145A (pt) | 2005-02-01 |
EP1498485A1 (en) | 2005-01-19 |
CA2481657A1 (en) | 2003-10-16 |
US20050216958A1 (en) | 2005-09-29 |
US20040110704A1 (en) | 2004-06-10 |
CN104530225A (zh) | 2015-04-22 |
PL373256A1 (en) | 2005-08-22 |
MXPA04009924A (es) | 2005-07-01 |
CN102911987B (zh) | 2015-09-30 |
EA200401325A1 (ru) | 2005-04-28 |
AU2003236022A1 (en) | 2003-10-20 |
HK1181815A1 (zh) | 2013-11-15 |
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CN1930288B (zh) | 2012-08-08 |
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