WO2001036444A1 - Plant developmental genes - Google Patents
Plant developmental genes Download PDFInfo
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- WO2001036444A1 WO2001036444A1 PCT/US2000/031325 US0031325W WO0136444A1 WO 2001036444 A1 WO2001036444 A1 WO 2001036444A1 US 0031325 W US0031325 W US 0031325W WO 0136444 A1 WO0136444 A1 WO 0136444A1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Definitions
- the polynucleotides of the invention encode plant transcription factors.
- the plant transcription factors are derived, e.g., from Arabidopsis thaliana and can belong, e.g., to one or more of the following transcription factor families: the AP2 (APETALA2) domain transcription factor family (Riechmann and Meyerowitz (1998) J. Biol. Chem. 379:633-646); the MYB transcription factor family (Martin and Paz-Ares (1997) Trends Genet. 13:67-73); the MADS domain transcription factor family (Riechmann and Meyerowitz (1997) J. Biol. Chem. 378:1079- 1101); the WRKY protein family (Ishiguro and Nakamura (1994) Mol. Gen. Genet.
- polynucleotides of the invention were ectopically expressed in overexpressor or knockout plants and changes in the structure and development characteristics of the plants were observed. Therefore, the polynucleotides and polypeptides can be employed to improve the structure and development characteristics of plants.
- washes can be performed at a lower temperature, e.g., 50° C.
- stringency is increased by raising the wash temperature and/or decreasing the concentration of SSC.
- polynucleotide sequences of the present invention can also be engineered in order to alter a coding sequence for a variety of reasons, including but not limited to, alterations which modify the sequence to facilitate cloning, processing and/or expression of the gene product.
- alterations are optionally introduced using techniques which are well known in the art, e.g., site-directed mutagenesis, to insert new restriction sites, to alter glycosylation patterns, to change codon preference, to introduce splice sites, etc.
- a fragment or domain derived from any of the polypeptides of the invention can be combined with domains derived from other transcription factors or synthetic domains to modify the biological activity of a transcription factor.
- Non-limiting examples of a modified amino acid include incorporation or other use of acetylated amino acids, glycosylated amino acids, sulfated amino acids, prenylated (e.g., farnesylated, geranylgeranylated) amino acids, PEG modified (e.g., "PEGylated”) amino acids, biotinylated amino acids, carboxylated amino acids, phosphorylated amino acids, etc.
- PEG modified e.g., "PEGylated”
- the invention provides a method for identifying compositions that modulate the activity or expression of a polynucleotide or polypeptide of the invention.
- a test compound whether a small or large molecule, is placed in contact with a cell, plant (or plant tissue or explant), or composition comprising the polynucleotide or polypeptide of interest and a resulting effect on the cell, plant, (or tissue or explant) or composition is evaluated by monitoring, either directly or indirectly, one or more of: expression level of the polynucleotide or polypeptide, activity (or modulation of the activity) of the polynucleotide or polypeptide.
- the polynucleotides may be used as probes, primers, sense and antisense agents, and the like, according to methods as noted supra.
- Another method for abolishing the expression of a gene is by insertion mutagenesis using the T-DNA of Agrobacterium tumefaciens. After generating the insertion mutants, the mutants can be screened to identify those containing the insertion in a transcription factor or transcription factor homologue gene. Plants containing a single transgene insertion event at the desired gene can be crossed to generate homozygous plants for the mutation (Koncz et al. (1992) Methods in Arabidopsis Research, World Scientific).
- the transcriptional machinery in a plant can be modified so as to increase transcription levels of a polynucleotide of the invention (See, e.g., PCT Publications WO 96/06166 and WO 98/53057 which describe the modification of the DNA binding specificity of zinc finger proteins by changing particular amino acids in the DNA binding motif).
- the control sequences can be detected by the query to ensure the general integrity of both the database and the query.
- the query can be performed using a web browser based interface.
- the database can be a centralized public database such as those noted herein, and the querying can be done from a remote terminal or computer across an internet or intranet.
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Macromonomer-Based Addition Polymer (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Steroid Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
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CA002391443A CA2391443A1 (en) | 1999-11-17 | 2000-11-14 | Plant developmental genes |
MXPA02004882A MXPA02004882A (en) | 1999-11-17 | 2000-11-14 | Plant developmental genes. |
EP00978661A EP1230256A4 (en) | 1999-11-17 | 2000-11-14 | Plant developmental genes |
BR0015634-5A BR0015634A (en) | 1999-11-17 | 2000-11-14 | Plant, polynucleotide, vector, cell, composition, polypeptide, methods for producing a plant and for identifying a factor that is modulated by, or interacts with, a polypeptide encoded by a polynucleotide, a molecule that modulates the activity or expression of a polynucleotide or polypeptide of interest and a sequence similar or homologous to one or more polynucleotides and an integrated system, computerized or legible in computer |
AU16101/01A AU1610101A (en) | 1999-11-17 | 2000-11-14 | Plant developmental genes |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
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US16622899P | 1999-11-17 | 1999-11-17 | |
US60/166,228 | 1999-11-17 | ||
US19789900P | 2000-04-17 | 2000-04-17 | |
US60/197,899 | 2000-04-17 | ||
US22743900P | 2000-08-22 | 2000-08-22 | |
US60/227,439 | 2000-08-22 |
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WO2001036444A1 true WO2001036444A1 (en) | 2001-05-25 |
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PCT/US2000/031344 WO2001036597A1 (en) | 1999-11-17 | 2000-11-14 | Plant biochemistry-related genes |
PCT/US2000/031325 WO2001036444A1 (en) | 1999-11-17 | 2000-11-14 | Plant developmental genes |
PCT/US2000/031458 WO2001036598A1 (en) | 1999-11-17 | 2000-11-14 | Environmental stress tolerance genes |
PCT/US2000/031418 WO2001035726A1 (en) | 1999-11-17 | 2000-11-14 | Pathogen tolerance genes |
PCT/US2000/031457 WO2001035727A1 (en) | 1999-11-17 | 2000-11-14 | Seed trait genes |
PCT/US2000/031414 WO2001035725A1 (en) | 1999-11-17 | 2000-11-14 | Yield-related genes |
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PCT/US2000/031458 WO2001036598A1 (en) | 1999-11-17 | 2000-11-14 | Environmental stress tolerance genes |
PCT/US2000/031418 WO2001035726A1 (en) | 1999-11-17 | 2000-11-14 | Pathogen tolerance genes |
PCT/US2000/031457 WO2001035727A1 (en) | 1999-11-17 | 2000-11-14 | Seed trait genes |
PCT/US2000/031414 WO2001035725A1 (en) | 1999-11-17 | 2000-11-14 | Yield-related genes |
Country Status (11)
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EP (7) | EP1230345B1 (en) |
AT (2) | ATE531804T1 (en) |
AU (6) | AU1919901A (en) |
BR (6) | BR0015634A (en) |
CA (6) | CA2391443A1 (en) |
DE (1) | DE60039184D1 (en) |
DK (1) | DK1230345T3 (en) |
ES (1) | ES2307542T3 (en) |
MX (6) | MXPA02004881A (en) |
PT (1) | PT1230345E (en) |
WO (6) | WO2001036597A1 (en) |
Cited By (50)
Publication number | Priority date | Publication date | Assignee | Title |
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DE10063986A1 (en) * | 2000-12-21 | 2002-06-27 | Max Planck Gesellschaft | Plants with improved resistance |
EP1381268A2 (en) * | 2001-03-30 | 2004-01-21 | Mendel Biotechnology, Inc. | Method for modifying plant biomass |
EP1447410A1 (en) * | 2003-02-06 | 2004-08-18 | Universiteit Leiden | Control of plant growth and developmental processes |
WO2005030966A3 (en) * | 2003-09-23 | 2005-09-01 | Neal I Gutterson | Regulation of plant biomass and stress tolerance |
WO2009084999A1 (en) | 2007-12-28 | 2009-07-09 | Swetree Technologies Ab | Woody plants having improved growth charateristics and method for making the same using transcription factors |
WO2010102220A1 (en) | 2009-03-05 | 2010-09-10 | Metabolix, Inc. | Propagation of transgenic plants |
WO2010147825A1 (en) | 2009-06-09 | 2010-12-23 | Pioneer Hi-Bred International, Inc. | Early endosperm promoter and methods of use |
US7888557B2 (en) | 2003-02-22 | 2011-02-15 | Monsanto Technology Llc | Transgenic plants |
WO2011036569A2 (en) | 2009-09-22 | 2011-03-31 | The Board Of Trustees Of The University Of Arkansas, N.A. | Rice cultivar cl 142-ar |
WO2011047191A1 (en) | 2009-10-15 | 2011-04-21 | The Board Of Trustees Of The University Of Arkansas, N.A. | Rice cultivar cl181-ar |
WO2011056544A1 (en) | 2009-10-26 | 2011-05-12 | Pioneer Hi-Bred International, Inc. | Somatic ovule specific promoter and methods of use |
US7964774B2 (en) | 2008-05-14 | 2011-06-21 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV384196 |
EP2397031A1 (en) * | 1999-11-10 | 2011-12-21 | The University Of Washington | Compositions and methods for modulation of plant cell division |
US8283519B2 (en) | 1998-09-22 | 2012-10-09 | Mendel Biotechnology, Inc. | Plant transcriptional regulators of abiotic stress |
WO2013096810A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11482 |
WO2013096818A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11268 |
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