WO2000063432A1 - Method for obtaining a plant with a lasting resistance to a pathogen - Google Patents
Method for obtaining a plant with a lasting resistance to a pathogenInfo
- Publication number
- WO2000063432A1 WO2000063432A1 PCT/NL2000/000241 NL0000241W WO0063432A1 WO 2000063432 A1 WO2000063432 A1 WO 2000063432A1 NL 0000241 W NL0000241 W NL 0000241W WO 0063432 A1 WO0063432 A1 WO 0063432A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- lettuce
- plant
- resistance genes
- dna
- resistance
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 39
- 230000002045 lasting effect Effects 0.000 title claims abstract description 18
- 244000052769 pathogen Species 0.000 title claims abstract description 17
- 230000001717 pathogenic effect Effects 0.000 title claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 149
- 241000196324 Embryophyta Species 0.000 claims abstract description 97
- 240000008415 Lactuca sativa Species 0.000 claims abstract description 89
- 241000233685 Bremia lactucae Species 0.000 claims abstract description 38
- 239000003550 marker Substances 0.000 claims description 43
- 235000010702 Insulata Nutrition 0.000 claims description 28
- 244000165077 Insulata Species 0.000 claims description 28
- 235000003228 Lactuca sativa Nutrition 0.000 claims description 28
- 125000005647 linker group Chemical group 0.000 claims description 24
- 240000005993 Lactuca saligna Species 0.000 claims description 18
- 241001293495 Lactuca virosa Species 0.000 claims description 12
- 235000015802 Lactuca sativa var crispa Nutrition 0.000 claims description 9
- 240000006137 Lactuca serriola Species 0.000 claims description 9
- 240000004201 Lactuca sativa var. crispa Species 0.000 claims description 6
- 241000017163 Acephala Species 0.000 claims description 3
- 235000000318 Bindesalat Nutrition 0.000 claims description 3
- 241001249699 Capitata Species 0.000 claims description 3
- 241001245662 Eragrostis rigidior Species 0.000 claims description 3
- 241001381639 Lactuca sativa var. longifolia Species 0.000 claims description 3
- 244000046738 asparagus lettuce Species 0.000 claims description 3
- 235000006705 asparagus lettuce Nutrition 0.000 claims description 3
- 240000004360 Abelmoschus ficulneus Species 0.000 claims description 2
- 241001293493 Lactuca altaica Species 0.000 claims description 2
- 241001293483 Lactuca tenerrima Species 0.000 claims description 2
- 241001293486 Lactuca viminea Species 0.000 claims description 2
- 244000153390 Lactuca indica Species 0.000 claims 1
- 241000375392 Tana Species 0.000 claims 1
- 241000208822 Lactuca Species 0.000 description 23
- 238000004458 analytical method Methods 0.000 description 13
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- 230000008261 resistance mechanism Effects 0.000 description 2
- 230000028070 sporulation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233684 Bremia Species 0.000 description 1
- 240000004160 Capsicum annuum Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
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- 241001461023 Oidium lycopersici Species 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Definitions
- the present invention relates to a method for obtaining a plant with a lasting resistance to a pathogen.
- the invention also relates to a plant in which two or more resistance genes to the pathogen are present, in addition to seeds and progeny of this plant, and progeny thereof .
- the invention relates particularly to a method for obtaining a cultivated lettuce plant (L. sativa) with a lasting resistance to Bremia lactucae.
- the invention also relates to DNA-markers which are specifically linked to a resistance gene to Bremia lactucae.
- the invention further relates to a cultivated lettuce plant (L. sativa) in which two or more Dm-resistance genes are present, and to seeds and progeny of this plant, and progeny thereof.
- the disease which is caused by the fungus Bremia lactucae Regel is known as downy mildew. Downy mildew occurs worldwide and represents a great problem for both the yield and quality of cultivated lettuce.
- the fungus can infect the lettuce plant at any stage of growth, after which the first symptoms of downy mildew consist of the appearance of chlorotic yellow spots on the leaf surface. Within 24 to 48 hours a white fluffy fungus growth then becomes visible on the lower leaf surface as an indication of spore formation. During the infection the lesions become increasingly larger and more chlorotic until the leaves become completely brown.
- Bremia lactucae is one of the so-called Oo ycetes, a class of relatively primitive fungi. Other known fungi of this group are for instance Phvtium and Phvtophtora .
- the fungus B. lactucae contains different physiological species ("physios") and is host-specific. Bremia lactucae is known as a very variable pathogen. New physios occur relatively frequently through mutation of the avirulence genes during the spore formation preceding the propagation of B. lactucae. Within the Lactucae genus, to which the cultivated lettuce (Lactuca sativa) belongs, there are different species which are resistant to Bremia lactucae Regel.
- Dm Downy mildew
- the resistance mechanism is known as a gene-for-gene working principle based on the specific interaction between products of the Dm-resistance gene and the pathogen- specific avirulence gene, which results in resistance of the lettuce plant (Michelmore et al., Plant Pathology 33, 301-315, 1984). This resistance mechanism has also been demonstrated for diverse other resistance genes in different other plant species (Michelmore et al., Genome Research, 8, 1113-1130, 1998).
- a large number of Dm-resistance genes have already been identified which can bring about resistance to specific physios of Bremia lactucae Regel.
- Dm-resistance genes often occur clustered in groups on the same chromosome.
- Four such linking groups on different chromosomes in the genome of lettuce have been demonstrated which contain different Dm-resistance genes (Farrara et al., Plant Pathology 36, 499-514, 1987).
- Newly identified Dm-genes can often be classified into one of the known resistance linking groups.
- Crossed-in Dm-resistance genes have been demonstrated in conventio- nal manner by means of an artificial Bremia lactucae disease test.
- a number of leaf punches - (diameter 18-20 mm) or seedlings of the lettuce plant are inoculated with different physios of B. lactucae. After 10 to 14 days the degree of development and sporulation on the punches/seedlings is then examined. On the basis hereof it is possible to judge whether a tested lettuce plant or improved line is resistant or susceptible to the tested B. lactucae physios.
- the general object of the present invention is therefore to provide a method for obtaining a plant with a lasting resistance to a pathogen.
- a particular objective of the present invention is to provide a method for obtaining a cultivated lettuce plant (L. sativa) with a lasting resistance to B. lactucae.
- the invention provides for this purpose a method for obtaining a plant with a lasting resistance to a pathogen, comprising of providing one or more specific DNA-markers linked to one or more resistance genes, determining the presence of one or more resistance genes in a plant using these DNA-markers, subsequently crossing a first plant comprising one or more resistance genes with a second plant comprising one or more resistance and selecting from the progeny a plant in which two or more resistance genes are present using the DNA-markers.
- the present invention particularly provides a method for obtaining a cultivated lettuce plant (L. sativa) with a lasting resistance to Bremia lactucae, comprising of providing one or more specific DNA-markers linked to one or more Dm-resistance genes, determining the presence of one or more Dm-resistance genes in a cultivated lettuce plant and/or wild lettuce plant using these DNA-markers, subsequently crossing a cultivated lettuce plant comprising at least one or more Dm-resistance genes with another cultivated lettuce plant or a wild lettuce plant comprising at least one or more Dm-resistance genes, and selecting from the progeny thereof a cultivated lettuce - plant with two or more Dm-resistance genes using the DNA- markers.
- plants particularly cultivated lettuce plants, can be obtained in simple manner which comprise two or more resistance genes, particularly two or more Dm-resistance genes, with a lasting resistance to a pathogen, particularly Bremia lactucae.
- the selection of plants in which two or more qualitative resistance genes are present can only be accomplished using molecular DNA-markers which can demonstrate the specific genes in the genome of the lettuce plant. With the conventional disease tests it is not possible to demonstrate the presence of two or more qualitative resistance genes in a cultivated lettuce plant.
- the method according to invention can also be used for quantitative resistance genes.
- the resistance genes are preferably qualitative resistance genes, and the resistance genes are preferably located in different linking groups.
- DNA-markers linked to the resistance genes Use can be made herefor of different DNA-markers such as for instance RAPD (random amplified polymorphic DNA) , AFLP (amplified fragment length polymorphism) , SCAR (sequence characterized amplified region) etc.
- RAPD random amplified polymorphic DNA
- AFLP amplified fragment length polymorphism
- SCAR sequence characterized amplified region
- DNA-markers have been found for four Dm-resistance genes, particularly qualitative broad-spectrum Dm-resistance genes from the Lactuca family. Using these DNA-markers it has been established that the four Dm-resistance genes are located in separate linking groups, whereby stacking of the Dm- resistance genes in cultivated lettuce (L. sativa) is possible.
- the position of the DNA-markers can be determined by generating a so-called genetic map or by studying the dependent or independent segregation of the different DNA-markers in relation to each other.
- RAPD-primers OPA-01 to OPAN-20, Operon Technologies, Alameda, USA; UBC 1 to 800, University of British Columbia, Vancouver, Canada
- RAPD analysis is a per se known technique (Williams et al., Nucleic Acids Research, 18, 6531-6535, 1990) based on the use of primers with a random sequence for the purpose of amplifying random segments of the genomic DNA.
- polymorphisms can then be demonstrated on an agarose gel and can be used as genetic markers .
- 1600 primers from Operon technologies, and the University of British Columbia, UBC 1 to 800 were used for the study.
- the DNA of the plants was mixed with the primers in a suitable amplification mixture and subsequently amplified.
- the amplification products were analysed on an agarose gel for the presence of suitable DNA-markers .
- the 'candidate' molecular DNA-markers found in the RAPD-analysis were tested on the individuals of the segregating population, whereafter it was possible to establish which of these DNA-markers were physically linked in suitable manner to the different studied qualitative Dm-resistance genes.
- DNA-marker A (primer OPAF06, 451 bp) ; DNA-marker B (primer OPAM10, 555 bp) ; DNA-marker Cl (primer OPW16, 750 bp) , DNA-marker C2 (primer OPL03, 276 bp) , DNA-marker C3 (primer OPAE19, 675 bp) and DNA-marker C4 (primer UBC711, 1083 bp) ; and DNA- marker Dl (primer OPW04, 520 bp) and DNA-marker D2 (primer OPW19, 963 bp) .
- the sequence of the markers A, B, C2 , C3 , C4 and D2 was then determined and are shown in figures 1-6.
- the DNA-markers found were subsequently used to select a cultivated lettuce plant with two or more Dm- resistance genes, after introgression of the resistance genes from wild lettuce species, such as for instance Lactuca virosa and L. serriola.
- the crossing into cultivated lettuce varieties of two or more resistance genes, particularly qualitative broad-spectrum Dm- resistance genes, from wild lettuce species, such as for instance L. virosa, has not been described previously.
- the wild lettuce plant used for the method according to invention can for instance be chosen from L. saligna, L. altaica, L. aculeata, L. homblei , L. indica, L. tenerrima, L. s ⁇ /uarrosa , L. viminea, L. augustana .
- the wild lettuce plant is preferably L. virosa or L. serriola, more preferably L. virosa.
- the method according to the invention is preferably used to stack qualitative resistance genes, such as Dm- resistance genes, in cultivated lettuce (L. sativa) .
- This further includes for instance head lettuce varieties (L. sativa Lineaus capitata) , such as iceberg lettuce, batavia lettuce and butterhead lettuce, varieties of leaf lettuce for picking (L. sativa Lineaus acephala) , such as curly leaf lettuce and stem lettuce, cos lettuce (L. sativa Lineaus romana) , leaf lettuce for cutting (L. sativa Lineaus secalina) and asparagus lettuce (L. sativa Lineaus angustana) .
- the method according to the invention for obtaining a plant with a lasting resistance to a pathogen, as described for cultivated lettuce can be used in analogous manner for other cultivated crops or other plants, and other pathogens.
- obtaining a lasting resistance to determined nematodes such as Meloidoqyne iavanica, M. arenaria. and M. incognita, or to Oidium lycopersici in tomato
- the present invention further provides DNA-markers which are specifically linked to a Dm-resistance gene, and which comprise a DNA-fragment with a sequence which is at least 70%, preferably at least 80%, more preferably at least 90%, and most preferably at least 95% homologous to a sequence as shown in any of the figures 1-6.
- the invention further relates to a plant in which two or more resistance genes to a pathogen are present, generally, and particularly to a cultivated lettuce plant (L. sativa) in which two or more Dm-resistance genes are present, and to the seeds and progeny of the plant, particularly the cultivated lettuce plant, or the progeny thereof.
- a cultivated lettuce plant L. sativa
- Dm-resistance genes are present
- a lasting resistance is thus understood to mean in the present invention that there are present in a plant at least two or more resistance genes, for instance two or more broad-spectrum Dm-resistance genes, to a pathogen.
- the pathogen is for instance B. lactucae, but can also be any other organism capable of causing disease in plants, such as for instance fungi, viruses, 5 nematodes, bacteria, (parasitic) insects etc.
- a Dm-resistance gene is a qualitative, broad-spectrum Dm-resistance gene to the fungus Bremia lactucae.
- Figures 1-6 show respectively the sequence of the DNA-markers A, B, C2 , C3 , C4 , D2 ; and 5 Figures 7-14 show eight DNA-markers according to the invention in 24 tested F2-individuals.
- Marker A was identified with primer OPAF06 (451 bp) ;
- marker B was identified using primer OPAM10 (555 bp) , marker Cl using primer OPW16 (750 bp) , marker C2 using primer OPL03 (276 0 bp) , marker C3 using primer OPAE19 (675 bp) and marker C4 using primer UBC711 (1083 bp;
- DNA-marker Dl was identified with primer OPW04 (520 bp) , and marker D2 with primer 0PW19 (963 bp) .
- DNA-markers were checked on individuals of the segregating population, whereafter it was possible to determine which of the DNA-markers were best physically linked to the examined qualitative Dm-resistance gene with a broad- spectrum resistance to B. lactucae.
- markers A were identified with primer OPAF06 (451 bp) ; marker B was identified using primer OPAM10 (555 bp) , marker Cl using primer OPW16 (750 bp) , marker C2 using primer OPL03 (276 bp) , marker C3 using primer 0PAE19 (675 bp) and marker C4 using primer UBC711 (1083 bp) ; DNA-marker Dl was identified with primer OPW04 (520 bp) , and marker D2 with primer 0PW19 (963 bp) .
- I resistant* BC1 plant X L. sativa i embryo-rescue I resistant* BC2 plant X L. sativa (iceberg lettuce type) (fertile) I resistant* BC3 plant self-pollination
- the BC3Z population was then tested and marker A identified. Individual BC3Z plants were self-pollinated and from the BC3Z2 populations the individual BC3Z2 plants homozygous for gene A were selected. The selected plant was used for linking analysis of the diverse identified DNA-markers (Example 3) .
- the BC3Z population was tested and marker B identified. Individual BC3Z plants were self-pollinated and from the obtained BC3Z2 populations the individual BC3Z2 plants homozygous for gene B were selected and used for linking analysis of the diverse identified DNA- markers (Example 3) .
- L. sativa X L. virosa (butterhead lettuce type) CGN5148 (IVT1583)
- the BC3Z population was tested and markers Cl, C2 , C3 and C4 identified.
- the individual BC3Z plants were self-pollinated and from the BC3Z2 populations the individual BC3Z2 plants homozygous for gene C were selected and used for linking analysis of the diverse identified DNA-markers (Example 3) .
- the BC3Z population was tested and markers Dl and D2 identified.
- the individual BC3Z plants were self- pollinated and from the BC3Z2 populations the individual BC3Z2 plants homozygous for gene D were selected and used for linking analysis of the diverse identified DNA- markers (Example 3).
- Determining of the position of DNA-markers can be carried out by generating a genetic map of the 9 chromosomes of lettuce.
- crossings are made between lettuce plants which are highly polymorphic relative to each other from a genetic viewpoint. For this type of crossing with a high degree of polymorphism a distinction can be made between:
- An F2 or BC1 population is generated of both types of crossing.
- all plants can be individually analysed for the presence or absence of the polymorphic molecular DNA-markers.
- JoinMap Seam, Plant Journal 3, 739-744, 1993
- linking groups can be constructed which place the diverse tested DNA- markers linearly relative to each other, separated by specific recombination distances denoted in centiMorgans.
- the broad-spectrum Dm- resistance gene can, after testing with B. lactucae , be placed within one of the linking groups shown on a detailed genetic map of lettuce.
- a genetic lettuce map with 9 linking groups has been described by Michelmore
- Another method for determining the position of the DNA-markers as applied in the present invention linked to the resistance genes consists of studying the dependent or independent segregation of the different DNA-markers. Selected for this purpose from the four populations were individual plants which are homozygous for the specific broad spectrum Dm-resistance genes from respectively population A, B, C or D. Specific crossings were then made for the generation of a segregating F2 population in which all Dm-resistance genes and their corresponding DNA-markers were present.
- a plant homozygous for Dm-resistance gene A (as demonstrated with marker A) was crossed with a plant homozygous for Dm-resistance gene B (marker B) .
- the individual FI plant with both Dm-resistance gene A and B (after analysis with the DNA-markers A and B) , as well as the individual plants of the F2 population were subsequently self-pollinated.
- a plant homozygous for Dm-resistance gene C (as demonstrated with markers Cl, C2 , C3 or C4) was crossed with a plant homozygous for Dm-resistance gene D (markers Dl or D2) .
- the individual FI plant with both Dm- resistance gene C and D (after analysis with the DNA- markers Cl, C2 , C3 or C4 and Dl or D2) , as well as the individual plants of the F2 population were subsequently self-pollinated.
- a selection was made from the F3 populations of plants which were homozygous for Dm- resistance gene C and for Dm-resistance gene D, using the DNA-markers specific for the Dm-resistance genes C and D. Being able to select a plant with the qualitative Dm-resistance genes C and D each having a broad-spectrum Dm-resistance means that both resistance genes are localized in different linking groups.
- the selected plant homozygous for Dm-resistance genes A and B was then crossed with the selected plant homozygous for Dm-resistance genes C and D.
- the FI plants heterozygous for the Dm-resistance genes A, B, C and D were self-pollinated.
- the F2 population was tested in the B. lactucae disease test and analysed with the DNA-markers for the 4 broad-spectrum Dm-resistance genes.
- lactucae (fresh or frozen) which sporulates on leaf material, in a small measured quantity of water, mixing it and sieving this solution. The concentration of living spores was then determined by means of fluorescence microscopy and adjusted if necessary. The optimal spore concentration is 10,000- 50,000 virulent spores/ml water. The inoculum was applied to the punches or seedlings with a plant spray until the punches were slightly moist. The tray was then covered again with a glass plate and set aside at 12-16°C and 16 hours light and 8 hours of darkness. After 10 to 14 days it was possible to assess the punches for the degree of development and sporulation and it was possible to state whether a tested plant or lettuce number is resistant or susceptible to the tested B. lactucae physio.
- the DNA-marker analysis was performed as described in Example 1.
- Figures 7-14 show that the DNA-markers linked to the 4 broad-spectrum Dm-resistance genes segregate independently of each other and can thus be positioned in the four separate linking groups.
- Plants can hereby be selected which comprise at least 2 , preferably 3 , and most preferably 4 qualitative resistance genes (indicated with: * in table 1 below) , have a broad-spectrum Dm- resistance and are therefore valuable for processing to a commercial lettuce variety.
- Table 1 RAPD-markers originating from 4 different 5 linkage groups (chromosomes) .
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Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00923018A EP1179089B1 (en) | 1999-04-16 | 2000-04-13 | Method for obtaining a plant with a lasting resistance to a pathogen |
AU43204/00A AU4320400A (en) | 1999-04-16 | 2000-04-13 | Method for obtaining a plant with a lasting resistance to a pathogen |
DE60028760T DE60028760T2 (en) | 1999-04-16 | 2000-04-13 | PROCESS FOR OBTAINING A PLANT WITH CONTINUOUS RESISTANCE TO A PATHOGEN |
US09/959,037 US6903249B2 (en) | 1999-04-16 | 2000-04-13 | Lettuce plants having broad-spectrum Dm-resistance genes |
US11/146,392 US7501555B2 (en) | 1999-04-16 | 2005-06-06 | Method for obtaining a plant with a lasting resistance to a pathogen |
US12/362,556 US7790948B2 (en) | 1999-04-16 | 2009-01-30 | Method for obtaining a plant with a lasting resistance to a pathogen |
US12/853,881 US20100299777A1 (en) | 1999-04-16 | 2010-08-10 | Method for obtaining a plant with a lasting resistance to a pathogen |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NL1011819 | 1999-04-16 | ||
NL1011819A NL1011819C2 (en) | 1999-04-16 | 1999-04-16 | Method for obtaining a plant with a durable resistance to a pathogen. |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09959037 A-371-Of-International | 2000-04-13 | ||
US11/146,392 Division US7501555B2 (en) | 1999-04-16 | 2005-06-06 | Method for obtaining a plant with a lasting resistance to a pathogen |
Publications (1)
Publication Number | Publication Date |
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WO2000063432A1 true WO2000063432A1 (en) | 2000-10-26 |
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ID=19769030
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/NL2000/000241 WO2000063432A1 (en) | 1999-04-16 | 2000-04-13 | Method for obtaining a plant with a lasting resistance to a pathogen |
Country Status (8)
Country | Link |
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US (4) | US6903249B2 (en) |
EP (1) | EP1179089B1 (en) |
AT (1) | ATE330034T1 (en) |
AU (1) | AU4320400A (en) |
DE (1) | DE60028760T2 (en) |
ES (1) | ES2264932T3 (en) |
NL (1) | NL1011819C2 (en) |
WO (1) | WO2000063432A1 (en) |
Cited By (57)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2002050294A1 (en) * | 2000-12-19 | 2002-06-27 | Danmarks Jordbrugsforskning | A novel tissue specific plant promoter |
NL1019596C2 (en) * | 2001-12-18 | 2003-06-19 | Helmut Nellen | Cytoplasmic resistance to the pathogenic fungus Bremia lactucae in Lactuca species, especially lettuce |
WO2008021413A1 (en) * | 2006-08-15 | 2008-02-21 | Monsanto Technology Llc | Compositions and methods of plant breeding using high density marker information |
WO2012059497A1 (en) | 2010-11-02 | 2012-05-10 | Bayer Cropscience Ag | N-hetarylmethyl pyrazolylcarboxamides |
WO2012089757A1 (en) | 2010-12-29 | 2012-07-05 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
JP2012531920A (en) * | 2009-07-06 | 2012-12-13 | シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト | Pathogen resistant plant |
USRE44698E1 (en) * | 2004-06-16 | 2014-01-07 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Plants with reduced susceptibility to pathogenic oomycetes |
US8692074B2 (en) | 2011-07-28 | 2014-04-08 | Enza Zaden Beheer B.V. | Lettuce cultivar Denali |
US8692075B2 (en) | 2011-09-28 | 2014-04-08 | Enza Zaden Beheer B.V. | Lettuce cultivar Borromini |
US8722072B2 (en) | 2010-01-22 | 2014-05-13 | Bayer Intellectual Property Gmbh | Acaricidal and/or insecticidal active ingredient combinations |
US8766039B2 (en) | 2011-07-22 | 2014-07-01 | Enza Zaden Beheer B.V. | Lettuce cultivar Gaviota |
US8772580B2 (en) | 2012-04-17 | 2014-07-08 | Enza Zaden Beheer B.V. | Cuervo lettuce variety |
NL1040073C2 (en) * | 2013-02-28 | 2014-09-01 | Agrisemen B V | Lactuca sativa with bremia lactucae (downy mildew) resistance. |
US8822763B2 (en) | 2012-05-02 | 2014-09-02 | Enza Zaden Beheer B.V. | Pommegranate crunch lettuce variety |
US8847019B2 (en) | 2012-05-14 | 2014-09-30 | Enza Zaden Beheer B.V. | Capulin lettuce variety |
US8957284B2 (en) | 2012-05-23 | 2015-02-17 | Enza Zaden Beheer B.V. | Holon lettuce variety |
US8962928B2 (en) | 2012-08-16 | 2015-02-24 | Enza Zaden Beheer B.V. | Bandelier lettuce variety |
US8962927B2 (en) | 2012-06-15 | 2015-02-24 | Enza Zaden Beheer B.V. | Ansar lettuce variety |
US8987560B2 (en) | 2012-06-15 | 2015-03-24 | Enza Zaden Beheer B.V. | Poloma lettuce variety |
US8987559B2 (en) | 2012-06-11 | 2015-03-24 | Enza Zaden Beheer B.V. | Mirlo lettuce variety |
US8993848B2 (en) | 2012-06-18 | 2015-03-31 | Enza Zaden Beheer B.V. | Poneloya lettuce variety |
US8993849B2 (en) | 2012-07-31 | 2015-03-31 | Enza Zaden Beheer B.V. | Truchas lettuce variety |
US8993850B2 (en) | 2013-03-11 | 2015-03-31 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01L.1935’ |
US9000268B2 (en) | 2012-07-30 | 2015-04-07 | Enza Zaden Beheer B.V. | Tamarindo lettuce variety |
US9113609B2 (en) | 2013-03-11 | 2015-08-25 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01L.1937’ |
US9179638B2 (en) | 2013-03-12 | 2015-11-10 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01L30144’ |
US9198394B2 (en) | 2013-03-14 | 2015-12-01 | Enza Zaden Beheer B.V. | Lettuce variety ‘ arroyo’ |
US9206137B2 (en) | 2010-11-15 | 2015-12-08 | Bayer Intellectual Property Gmbh | N-Aryl pyrazole(thio)carboxamides |
US9265252B2 (en) | 2011-08-10 | 2016-02-23 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
US9277726B2 (en) | 2013-07-10 | 2016-03-08 | Enza Zaden Beheer B.V. | Pennylea lettuce variety |
US9313994B2 (en) | 2013-12-16 | 2016-04-19 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01K7719’ |
US9332725B2 (en) | 2014-04-14 | 2016-05-10 | Enza Zaden Beheer B.V. | Lettuce variety ‘red mist’ |
US9392765B2 (en) | 2014-06-25 | 2016-07-19 | Enza Zaden Beheer B.V. | Lettuce variety ‘Celinet’ |
US9572321B2 (en) | 2015-01-12 | 2017-02-21 | Enza Zaden Beheer B.V. | Lettuce varieties ‘Ezrilla’, ‘Analora’, and ‘Anandra’ |
US9635828B2 (en) | 2014-12-03 | 2017-05-02 | Enza Zaden Beheer B.V. | Lettuce variety ‘Newham’ |
US9743633B2 (en) | 2015-10-12 | 2017-08-29 | Enza Zaden Beheer B.V. | Lettuce varieties ‘Tuolomne’, ‘Rainier’ and ‘E01G70048’ |
US9961873B2 (en) | 2015-11-20 | 2018-05-08 | Enza Zaden Beheer B.V. | Lettuce varieties ‘mezquite’ and ‘clouny’ |
US10015948B2 (en) | 2016-03-09 | 2018-07-10 | Enza Zaden Beheer B.V. | Lettuce varieties ‘cristabel’, ‘crispinet’, and ‘fairly’ |
US10405510B2 (en) | 2017-04-19 | 2019-09-10 | Enza Zaden Beheer B.V. | Lettuce variety ‘Icemaker’ |
US10506773B2 (en) | 2017-02-10 | 2019-12-17 | Enza Zaden Beheer B.V. | Lettuce variety ‘weaverville’ |
US10517248B2 (en) | 2017-03-03 | 2019-12-31 | Enza Zaden Beheer B.V. | Lettuce variety ‘Somerset’ |
US10542698B2 (en) | 2017-02-27 | 2020-01-28 | Enza Zaden Beheer B.V. | Lettuce variety ‘Brentwood’ |
US10582681B2 (en) | 2016-03-02 | 2020-03-10 | Enza Zaden Beheer B.V. | Lettuce varieties ‘Bayfield’ and ‘Pueblo’ |
US10757880B2 (en) | 2017-07-31 | 2020-09-01 | Enza Zaden Beheer B.V. | Lettuce variety ‘Cavendish’ |
US10874071B2 (en) | 2017-12-21 | 2020-12-29 | Enza Zaden Beheer B.V. | Machine harvestable iceberg lettuce |
EP3808170A1 (en) | 2019-10-17 | 2021-04-21 | Bejo Zaden B.V. | Lactuca sativa resistance to bremia lactucae |
US11089751B2 (en) | 2017-05-18 | 2021-08-17 | Enza Zaden Beheer B.V. | Lettuce varieties ‘Ezthana’ and ‘Eztron’ |
US11102943B2 (en) | 2018-09-17 | 2021-08-31 | Enza Zaden Beheer B.V. | Lettuce variety ‘milagro’ |
US11337390B2 (en) | 2020-02-14 | 2022-05-24 | Enza Zaden Beheer B.V. | Lettuce variety ‘Roscoe’ |
US11350584B1 (en) | 2021-02-03 | 2022-06-07 | Enza Zaden Beheer B.V. | Lettuce variety ‘Airton’ |
US11490579B2 (en) | 2019-08-08 | 2022-11-08 | Enza Zaden Beheer B.V. | Lettuce variety ‘Casey’ |
US11559017B2 (en) | 2021-05-25 | 2023-01-24 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01L.30617’ |
WO2023051902A1 (en) | 2021-09-29 | 2023-04-06 | Bejo Zaden B.V. | Lettuce plants with bremia-resistance providing genomic fragments from lactuca serriola |
US11678622B2 (en) | 2020-05-08 | 2023-06-20 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01G11244’ |
US11778966B2 (en) | 2020-10-21 | 2023-10-10 | Enza Zaden Beheer B.V. | Lettuce variety ‘Marciano’ |
US11864514B2 (en) | 2020-12-16 | 2024-01-09 | Enza Zaden Beheer B.V. | Lettuce variety ‘Kailua’ |
US11944054B2 (en) | 2021-01-19 | 2024-04-02 | Enza Zaden Beheer B.V. | Lettuce variety ‘Newcastle’ |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7790962B2 (en) * | 2005-07-11 | 2010-09-07 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Downy mildew resistant lettuce |
WO2022008422A1 (en) * | 2020-07-06 | 2022-01-13 | Syngenta Crop Protection Ag | Bremia lactucae resistance sg01 |
-
1999
- 1999-04-16 NL NL1011819A patent/NL1011819C2/en not_active IP Right Cessation
-
2000
- 2000-04-13 ES ES00923018T patent/ES2264932T3/en not_active Expired - Lifetime
- 2000-04-13 DE DE60028760T patent/DE60028760T2/en not_active Expired - Lifetime
- 2000-04-13 AU AU43204/00A patent/AU4320400A/en not_active Abandoned
- 2000-04-13 AT AT00923018T patent/ATE330034T1/en not_active IP Right Cessation
- 2000-04-13 EP EP00923018A patent/EP1179089B1/en not_active Revoked
- 2000-04-13 US US09/959,037 patent/US6903249B2/en not_active Expired - Lifetime
- 2000-04-13 WO PCT/NL2000/000241 patent/WO2000063432A1/en active IP Right Grant
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2005
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2009
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-
2010
- 2010-08-10 US US12/853,881 patent/US20100299777A1/en not_active Abandoned
Non-Patent Citations (5)
Title |
---|
FARRARA B F ET AL: "IDENTIFICATION OF NEW SOURCES OF RESISTANCE TO DOWNY MILDEW IN LACTUCA SPP", HORTSCIENCE, vol. 22, no. 4, 1 August 1987 (1987-08-01), pages 647 - 649, XP000749877, ISSN: 0018-5345 * |
KESSELL R. ET AL.,: "Recessive resistance to plasmopara lactucae-radicis maps by bulked segregant analysis to a cluster of dominant disease resistance gens in lettuce", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 6, no. 6, - 1993, pages 722 - 728, XP002115628 * |
MICHELMORE R.W. ET AL.,: "Molecular markers and genome analysis in the manipulation of lettuce downy mildew", CURR. PLANT. SCI. BIOTECHNOL. AGRIC., vol. 14, - 1993, pages 517 - 523, XP002115627 * |
PARAN I ET AL: "DEVELOPMENT OF RELIABLE PCR-BASED MARKERS LINKED TO DOWNY MILDEW RESISTANCE GENES IN LETTUCE", THEORY OF APPLIED GENETICS, vol. 85, no. 8, 1993, pages 985 - 993, XP002913459 * |
PARAN I ET AL: "IDENTIFICATION OF RESTRICTION FRAGMENT LENGTH POLYMORPHISM AND RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS LINKED TO DOWNY MILDEW RESISTANCE GENES IN LETTUCE, USING NEAR-ISOGENIC LINES", GENOME, vol. 34, no. 6, 1991, pages 1021 - 1027, XP002913463, ISSN: 0831-2796 * |
Cited By (63)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2002050294A1 (en) * | 2000-12-19 | 2002-06-27 | Danmarks Jordbrugsforskning | A novel tissue specific plant promoter |
NL1019596C2 (en) * | 2001-12-18 | 2003-06-19 | Helmut Nellen | Cytoplasmic resistance to the pathogenic fungus Bremia lactucae in Lactuca species, especially lettuce |
USRE44698E1 (en) * | 2004-06-16 | 2014-01-07 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Plants with reduced susceptibility to pathogenic oomycetes |
WO2008021413A1 (en) * | 2006-08-15 | 2008-02-21 | Monsanto Technology Llc | Compositions and methods of plant breeding using high density marker information |
US10455783B2 (en) | 2006-08-15 | 2019-10-29 | Monsanto Technology Llc | Compositions and methods of plant breeding using high density marker information |
JP2012531920A (en) * | 2009-07-06 | 2012-12-13 | シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト | Pathogen resistant plant |
US8722072B2 (en) | 2010-01-22 | 2014-05-13 | Bayer Intellectual Property Gmbh | Acaricidal and/or insecticidal active ingredient combinations |
WO2012059497A1 (en) | 2010-11-02 | 2012-05-10 | Bayer Cropscience Ag | N-hetarylmethyl pyrazolylcarboxamides |
US9206137B2 (en) | 2010-11-15 | 2015-12-08 | Bayer Intellectual Property Gmbh | N-Aryl pyrazole(thio)carboxamides |
WO2012089757A1 (en) | 2010-12-29 | 2012-07-05 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
US8766039B2 (en) | 2011-07-22 | 2014-07-01 | Enza Zaden Beheer B.V. | Lettuce cultivar Gaviota |
US8692074B2 (en) | 2011-07-28 | 2014-04-08 | Enza Zaden Beheer B.V. | Lettuce cultivar Denali |
US9265252B2 (en) | 2011-08-10 | 2016-02-23 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
US8692075B2 (en) | 2011-09-28 | 2014-04-08 | Enza Zaden Beheer B.V. | Lettuce cultivar Borromini |
US8772580B2 (en) | 2012-04-17 | 2014-07-08 | Enza Zaden Beheer B.V. | Cuervo lettuce variety |
US8822763B2 (en) | 2012-05-02 | 2014-09-02 | Enza Zaden Beheer B.V. | Pommegranate crunch lettuce variety |
US8847019B2 (en) | 2012-05-14 | 2014-09-30 | Enza Zaden Beheer B.V. | Capulin lettuce variety |
US8957284B2 (en) | 2012-05-23 | 2015-02-17 | Enza Zaden Beheer B.V. | Holon lettuce variety |
US8987559B2 (en) | 2012-06-11 | 2015-03-24 | Enza Zaden Beheer B.V. | Mirlo lettuce variety |
US8962927B2 (en) | 2012-06-15 | 2015-02-24 | Enza Zaden Beheer B.V. | Ansar lettuce variety |
US8987560B2 (en) | 2012-06-15 | 2015-03-24 | Enza Zaden Beheer B.V. | Poloma lettuce variety |
US8993848B2 (en) | 2012-06-18 | 2015-03-31 | Enza Zaden Beheer B.V. | Poneloya lettuce variety |
US9000268B2 (en) | 2012-07-30 | 2015-04-07 | Enza Zaden Beheer B.V. | Tamarindo lettuce variety |
US8993849B2 (en) | 2012-07-31 | 2015-03-31 | Enza Zaden Beheer B.V. | Truchas lettuce variety |
US8962928B2 (en) | 2012-08-16 | 2015-02-24 | Enza Zaden Beheer B.V. | Bandelier lettuce variety |
WO2014131857A1 (en) * | 2013-02-28 | 2014-09-04 | Agrisemen Bv | Lactuca sativa with bremia lactucae (downy mildew) resistance |
NL1040073C2 (en) * | 2013-02-28 | 2014-09-01 | Agrisemen B V | Lactuca sativa with bremia lactucae (downy mildew) resistance. |
US10575479B2 (en) | 2013-02-28 | 2020-03-03 | Bejo Zaden Bv | Lactuca sativa with Bremia lactucae (Downy Mildew) resistance |
US8993850B2 (en) | 2013-03-11 | 2015-03-31 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01L.1935’ |
US9113609B2 (en) | 2013-03-11 | 2015-08-25 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01L.1937’ |
US9179638B2 (en) | 2013-03-12 | 2015-11-10 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01L30144’ |
US9198394B2 (en) | 2013-03-14 | 2015-12-01 | Enza Zaden Beheer B.V. | Lettuce variety ‘ arroyo’ |
US9277726B2 (en) | 2013-07-10 | 2016-03-08 | Enza Zaden Beheer B.V. | Pennylea lettuce variety |
US9313994B2 (en) | 2013-12-16 | 2016-04-19 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01K7719’ |
US9332725B2 (en) | 2014-04-14 | 2016-05-10 | Enza Zaden Beheer B.V. | Lettuce variety ‘red mist’ |
US9392765B2 (en) | 2014-06-25 | 2016-07-19 | Enza Zaden Beheer B.V. | Lettuce variety ‘Celinet’ |
US9635828B2 (en) | 2014-12-03 | 2017-05-02 | Enza Zaden Beheer B.V. | Lettuce variety ‘Newham’ |
US9572321B2 (en) | 2015-01-12 | 2017-02-21 | Enza Zaden Beheer B.V. | Lettuce varieties ‘Ezrilla’, ‘Analora’, and ‘Anandra’ |
US9743633B2 (en) | 2015-10-12 | 2017-08-29 | Enza Zaden Beheer B.V. | Lettuce varieties ‘Tuolomne’, ‘Rainier’ and ‘E01G70048’ |
US9961873B2 (en) | 2015-11-20 | 2018-05-08 | Enza Zaden Beheer B.V. | Lettuce varieties ‘mezquite’ and ‘clouny’ |
US10582681B2 (en) | 2016-03-02 | 2020-03-10 | Enza Zaden Beheer B.V. | Lettuce varieties ‘Bayfield’ and ‘Pueblo’ |
US10015948B2 (en) | 2016-03-09 | 2018-07-10 | Enza Zaden Beheer B.V. | Lettuce varieties ‘cristabel’, ‘crispinet’, and ‘fairly’ |
US10506773B2 (en) | 2017-02-10 | 2019-12-17 | Enza Zaden Beheer B.V. | Lettuce variety ‘weaverville’ |
US10542698B2 (en) | 2017-02-27 | 2020-01-28 | Enza Zaden Beheer B.V. | Lettuce variety ‘Brentwood’ |
US10517248B2 (en) | 2017-03-03 | 2019-12-31 | Enza Zaden Beheer B.V. | Lettuce variety ‘Somerset’ |
US10405510B2 (en) | 2017-04-19 | 2019-09-10 | Enza Zaden Beheer B.V. | Lettuce variety ‘Icemaker’ |
US11089751B2 (en) | 2017-05-18 | 2021-08-17 | Enza Zaden Beheer B.V. | Lettuce varieties ‘Ezthana’ and ‘Eztron’ |
US11490578B2 (en) | 2017-07-31 | 2022-11-08 | Enza Zaden Beheer B.V. | Lettuce variety ‘clearwater’ |
US10757880B2 (en) | 2017-07-31 | 2020-09-01 | Enza Zaden Beheer B.V. | Lettuce variety ‘Cavendish’ |
US11889805B2 (en) | 2017-07-31 | 2024-02-06 | Enza Zaden Beheer B.V. | Lettuce variety ‘Pensacola’ |
US10874071B2 (en) | 2017-12-21 | 2020-12-29 | Enza Zaden Beheer B.V. | Machine harvestable iceberg lettuce |
US11477955B2 (en) | 2017-12-21 | 2022-10-25 | Enza Zaden Beheer B.V. | Machine harvestable iceberg lettuce |
US11102943B2 (en) | 2018-09-17 | 2021-08-31 | Enza Zaden Beheer B.V. | Lettuce variety ‘milagro’ |
US11490579B2 (en) | 2019-08-08 | 2022-11-08 | Enza Zaden Beheer B.V. | Lettuce variety ‘Casey’ |
EP3808170A1 (en) | 2019-10-17 | 2021-04-21 | Bejo Zaden B.V. | Lactuca sativa resistance to bremia lactucae |
US11337390B2 (en) | 2020-02-14 | 2022-05-24 | Enza Zaden Beheer B.V. | Lettuce variety ‘Roscoe’ |
US11678622B2 (en) | 2020-05-08 | 2023-06-20 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01G11244’ |
US11778966B2 (en) | 2020-10-21 | 2023-10-10 | Enza Zaden Beheer B.V. | Lettuce variety ‘Marciano’ |
US11864514B2 (en) | 2020-12-16 | 2024-01-09 | Enza Zaden Beheer B.V. | Lettuce variety ‘Kailua’ |
US11944054B2 (en) | 2021-01-19 | 2024-04-02 | Enza Zaden Beheer B.V. | Lettuce variety ‘Newcastle’ |
US11350584B1 (en) | 2021-02-03 | 2022-06-07 | Enza Zaden Beheer B.V. | Lettuce variety ‘Airton’ |
US11559017B2 (en) | 2021-05-25 | 2023-01-24 | Enza Zaden Beheer B.V. | Lettuce variety ‘E01L.30617’ |
WO2023051902A1 (en) | 2021-09-29 | 2023-04-06 | Bejo Zaden B.V. | Lettuce plants with bremia-resistance providing genomic fragments from lactuca serriola |
Also Published As
Publication number | Publication date |
---|---|
EP1179089B1 (en) | 2006-06-14 |
EP1179089A1 (en) | 2002-02-13 |
US20060005272A1 (en) | 2006-01-05 |
DE60028760D1 (en) | 2006-07-27 |
US20040226060A1 (en) | 2004-11-11 |
US7790948B2 (en) | 2010-09-07 |
US20090271890A1 (en) | 2009-10-29 |
ES2264932T3 (en) | 2007-02-01 |
AU4320400A (en) | 2000-11-02 |
ATE330034T1 (en) | 2006-07-15 |
NL1011819C2 (en) | 2000-10-17 |
US7501555B2 (en) | 2009-03-10 |
US20100299777A1 (en) | 2010-11-25 |
US6903249B2 (en) | 2005-06-07 |
DE60028760T2 (en) | 2007-05-24 |
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